CN102630556A - Breeding method of multi-resistance glutinous rice restoring line - Google Patents
Breeding method of multi-resistance glutinous rice restoring line Download PDFInfo
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- CN102630556A CN102630556A CN201210145808XA CN201210145808A CN102630556A CN 102630556 A CN102630556 A CN 102630556A CN 201210145808X A CN201210145808X A CN 201210145808XA CN 201210145808 A CN201210145808 A CN 201210145808A CN 102630556 A CN102630556 A CN 102630556A
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Abstract
The invention discloses a breeding method of a multi-resistance glutinous rice restoring line, belonging to the technical field of rice disease-resistance breeding, which comprises the following steps that: a three-line glutinous restoring line is taken as a recurrent parent, and is respectively hybridized with a rice blast resource-resisting parent-A, a rice bacterial leaf blight resource-resisting parent-B, a bacterial leaf steak resource-resisting parent-C and a banded sclerotial blight-D, by a synchronous back-cross aggregation method, the resource-resisting genes of the leading four rice diseases at present can be aggregated to a good three-line glutinous hybridized restoring line of the same genotypic milieu, and the bred multi-resistance glutinous rice restoring line can simultaneously and highly resist the four diseases, and has other agricultural and yield traits which are consistent with those of the original parent, so that the disease resistance of the three-line glutinous hybridized rice can be greatly improved. When the method is used for preparing the glutinous hybridized rice, the disease resistance of the glutinous hybridized rice can be greatly improved.
Description
Technical field
The present invention is specifically related to a kind of wide anti-glutinous rice and recovers the selection of system, belongs to the paddy disease-resistant breeding technical field.
Background technology
The rice in China area under cultivation accounts for 30% of cereal crops, and gross yield accounts for more than 40% of cereal crops gross yield.Paddy rice makes a great contribution with raising people ' s health level to solving Chinese grain security as the first cereal crops of China.In recent years, China's rice disease is broken out frequently, and harm is serious; Anti-governor pressure is big, and Deposits in Eastern Coastal China, riverine and southern rice district rice disease take place heavier, and resistance development is very fast; The difficulty of chemical control strengthens, and the phenomenon that drops in production over a large area evil due to illness appears and in the part plot, and agricultural technology spread at different levels department has constantly put into effect some prophylactico-therapeutic measuress; Release a series of new drugs or new prescription etc. and carry out integrated control, obtained certain effect.Yet, in order to prevent and treat disease, constantly spray insecticide and not only cause serious grain contamination and environmental pollution, also strengthened kind grain input, drawn high provision price.Prevent and treat rice disease the most effectively, the way of economy and environmental protection is the disease-resistant rice varieties of seed selection, the breeding man of China also recognizes the importance of breeding for disease resistance in recent years, and selects a collection of disease resisting rice new varieties.But mostly domestic disease-resistant variety is the monoclonal antibody kind at present; Promptly only a certain disease there is resistance; Because it is big to be aggregated in the kind difficulty to the hybridization of several kinds of disease-resistant genes; Use traditional breeding method and import resistant gene one by one and take time and effort, weak effect is so the kind that possesses several kinds of disease resistances simultaneously seldom.The glutinous hybrid rice of applying on particularly producing at present, though output is fine, its disease resistance is poor especially, the due to illness harmful production loss that causes can reach 8-20%.It is in the glutinous hybrid rice restoring line with the 4 kinds of anti-source of present topmost rice disease gene pyramidings to good three of same genetic background that this method adopts the polymerization of backcrossing synchronously; Thereby improving three greatly is the disease resistance of glutinous hybrid rice; Also can reduce pollution when increasing output, significant to guaranteeing Rice Production and grain-production safety.
Summary of the invention
The object of the present invention is to provide a kind of wide anti-glutinous rice to recover the selection of system, is the disease resistance of glutinous hybrid rice thereby improve three greatly.
For reaching above purpose, the present invention adopts following technical scheme:
A kind of wide anti-glutinous rice of the present invention recovers the selection of system, and said selection may further comprise the steps:
1) be that glutinous recovery is to be recurrent parent with three, with rice blast resistant parent-A, bacterial leaf-blight resistant parent-B, the sick resistant parent-C of slice, banded sclerotial blight resistant parent-D hybridization, totally four combinations must be hybridized F respectively
1
2) plantation hybridization F
1Generation, F
2Generation, F
2Its disease resistance is identified that the disease-resistant kind of its corresponding antibodies parent is only identified in each combination for each combination, filtered out high anti-and agronomy individual plant and the recurrent parent close with the corresponding antibodies parent with yield traits backcrossed; Plant the BC that backcrosses
1F
1Generation, BC
1F
2Generation, BC
1F
2Make up each strain system for each its disease resistance is identified, the high anti-strain of screening system from each combination, and the high anti-individual plant of screening is backcrossed with recurrent parent from each high anti-strain system, gets BC
2F
1In generation, repeat above step to the BC that backcrosses
5F
1Generation, plantation BC
5F
1Generation, BC
5F
2In generation, screening at last obtains agronomy glutinous recovery similar with the corresponding antibodies parent with yield traits, that height resists corresponding disease and is, respectively with F
A, F
B, F
C, F
DExpression;
3) make hybrid combination F
A/ F
B, F
C/ F
D, plantation hybridization F
1Generation, F
2Generation, F
2For each hybrid combination its disease resistance is identified; Identify that its corresponding hybrid antibody parent resists 2 kinds of sick kinds, the high anti-and agronomy individual plant biparental cross close with recurrent parent of each individual plant that hybrid combination choosing height is anti-and agronomy is close with recurrent parent with yield traits and another hybrid combination with yield traits; Plantation biparental cross F
1Generation, F
2Generation, F
2For each biparental cross combination its disease resistance is identified, identified 4 kinds of sick kinds that all parents resist, the high anti-and agronomy individual plant close with recurrent parent of each biparental cross combined sorting with yield traits, obtaining wide anti-glutinous rice recovery is strain;
4) wide anti-glutinous rice being recovered is that the strain kind becomes plant, filters out the close and strain that yield traits is good of economical character and recurrent parent and breeds wide anti-glutinous recovery and be.
Three to be good glutinous extensive No. 2 of glutinous recovery system, to be recurrent parent good glutinous extensive No. 3, good glutinous extensive No. 4 or good glutinous extensive No. 5.
With the Chinese parasol tree farming is the rice blast resistant parent No. 1, is the bacterial leaf-blight resistant parent with CBB23, is the sick resistant parent of slice with Acc8558, is the banded sclerotial blight resistant parent with Jasmine85.
Beneficial effect of the present invention:
Effect of the present invention is that importing same glutinous rice recovery to the anti-source gene of control rice blast, bacterial leaf-blight, slice disease and banded sclerotial blight through the back cross breeding method respectively is in the kind; Breed the NIL that contains identical genetic background and different disease-resistant genes; Pass through the polymerization of hybridization in twos of NIL again; Being aggregated in same genetic background glutinous rice recovery to 4 kinds of disease-resistant genes is in the kind; The wide anti-glutinous rice recovery of breeding is not only can highly simultaneously resist 4 kinds of diseases, and other agronomy is that the parent is highly consistent with yield traits and the recovery of former glutinous rice, still possesses high yield, high-quality characteristic.Because of disease-resistant, the waxy hybrid rice that wide anti-glutinous rice recovers system's preparation can increase output 10-20% for every mu, reduces spraying pesticide cost 50-100 unit.This breeding method than tradition one by one introductory technique can save the 2-4 breeding time.
Embodiment
Embodiment 1:
(1) be that glutinous recovery system is recurrent parent (with the R representative) good glutinous extensive No. 2 with three; With No. 1, rice blast resistant parent Chinese parasol tree farming (with the A representative), bacterial leaf-blight resistant parent CBB23 (with the B representative), the sick resistant parent Acc8558 of slice (with the C representative), banded sclerotial blight resistant parent Jasmine85 (with the D representative) hybridization, must hybridize F respectively
1
(2) plantation hybridization F
1Generation, F
2Generation, F
2For each combined grow 2000 strain; And its disease resistance identified that (each combination only identify the disease-resistant kind of its corresponding antibodies parent), each combination select high anti-individual plant 10 strains (screening agronomy is with yield traits and praise glutinous extensive No. 2 close individual plants) and recurrent parent to backcross; Plant the BC that backcrosses
1F
1Generation, BC
1F
2Generation, BC
1F
2Making up each strain for each is plantation 1000 strains, and its disease resistance is identified it is 2 that high anti-strain is selected in each combination, and each strain system selects high anti-individual plant 5 strains to backcross with good glutinous extensive No. 2, gets BC
2F
1Generation; Repeat above step to the 5 generation (BC that backcross
5F
1), plantation BC
5F
1, BC
5F
2, screening and obtaining agronomy is (respectively with F with yield traits with good glutinous extensive No. 2 glutinous recoveries similar, high anti-corresponding disease
A, F
B, F
C, F
DExpression);
(3) make hybrid combination 1 (F
A/ F
B), the combination 2 (F
C/ F
D), plantation hybridization F
1Generation, F
2Generation, F
2For each combined grow 2000 strain; And its disease resistance identified (each combination identifies that its corresponding antibodies parent resist 2 kinds of disease kinds); High anti-individual plant 5 strains (screening agronomy and yield traits and good glutinous extensive No. 2 close individual plants) and another combination biparental cross (combination 1/ combination 2), plantation hybridization F are selected in each combination
1Generation, F
2Generation, F
2Each plants 2000 strains generation 5 combinations; And its disease resistance identified (identifying that all parents resist 4 kinds of sick kinds); High anti-individual plant 5 strains (screening agronomy and yield traits and good glutinous extensive No. 2 close individual plants) are selected in each combination, and obtaining the extensively anti-glutinous recovery of 25 strains is strain;
(4) be that the strain kind becomes plant with the extensively anti-glutinous recovery of 25 strains, with good glutinous extensive No. 2 be contrast, to agronomy and yield traits article ratio, it is good to filter out economical character, the high strain of output is promptly bred wide anti-glutinous recovery system and " is extensively resisted and praise glutinous extensive No. 2 "." wide anti-good glutinous extensive No. 2 " not only can high simultaneously anti-4 kinds of diseases, other agronomy and yield traits with praise glutinous extensive No. 2 highly consistent, still possess high yield, high-quality characteristic.Wide anti-good glutinous extensive No. 2 waxy hybrid rices with good agricultural wxA1 preparation make up all has resistance to rice blast, bacterial leaf-blight, slice disease and 4 kinds of diseases of banded sclerotial blight; (good agricultural wxA1/good glutinous extensive No. 2) compares with former combination; Every mu can increase output 12%, reduces by 60 yuan of spraying pesticide costs.
Embodiment 2:
(1) be that glutinous recovery system is recurrent parent (with the R representative) good glutinous extensive No. 3 with three; With No. 1, rice blast resistant parent Chinese parasol tree farming (with the A representative), bacterial leaf-blight resistant parent CBB23 (with the B representative), the sick resistant parent Acc8558 of slice (with the C representative), banded sclerotial blight resistant parent Jasmine85 (with the D representative) hybridization, must hybridize F respectively
1
(2) plantation hybridization F
1Generation, F
2Generation, F
2For each combined grow 2000 strain; And its disease resistance identified that (each combination only identify the disease-resistant kind of its corresponding antibodies parent), each combination select high anti-individual plant 10 strains (screening agronomy is with yield traits and praise glutinous extensive No. 3 close individual plants) and recurrent parent to backcross; Plant the BC that backcrosses
1F
1Generation, BC
1F
2Generation, BC
1F
2Making up each strain for each is plantation 1000 strains, and its disease resistance is identified it is 2 that high anti-strain is selected in each combination, and each strain system selects high anti-individual plant 5 strains to backcross with good glutinous extensive No. 3, gets BC
2F
1Generation; Repeat above step to the 5 generation (BC that backcross
5F
1), plantation BC
5F
1, BC
5F
2, screening and obtaining agronomy is (respectively with F with yield traits with good glutinous extensive No. 3 glutinous recoveries similar, high anti-corresponding disease
A, F
B, F
C, F
DExpression);
(3) make hybrid combination 1 (F
A/ F
B), the combination 2 (F
C/ F
D), plantation hybridization F
1Generation, F
2Generation, F
2For each combined grow 2000 strain; And its disease resistance identified (each combination identifies that its corresponding antibodies parent resist 2 kinds of disease kinds); High anti-individual plant 5 strains (screening agronomy and yield traits and good glutinous extensive No. 3 close individual plants) and another combination biparental cross (combination 1/ combination 2), plantation hybridization F are selected in each combination
1Generation, F
2Generation, F
2Each plants 2000 strains generation 5 combinations; And its disease resistance identified (identifying that all parents resist 4 kinds of sick kinds); High anti-individual plant 5 strains (screening agronomy and yield traits and good glutinous extensive No. 3 close individual plants) are selected in each combination, and obtaining the extensively anti-glutinous recovery of 25 strains is strain;
(4) be that the strain kind becomes plant with the extensively anti-glutinous recovery of 25 strains, with good glutinous extensive No. 3 be contrast, to agronomy and yield traits article ratio, it is good to filter out economical character, the high strain of output is promptly bred wide anti-glutinous recovery system and " is extensively resisted and praise glutinous extensive No. 3 "." wide anti-good glutinous extensive No. 3 " not only can high simultaneously anti-4 kinds of diseases, other agronomy and yield traits with praise glutinous extensive No. 3 highly consistent, still possess high yield, high-quality characteristic.Wide anti-good glutinous extensive No. 3 waxy hybrid rices with good agricultural wxA1 preparation make up all has resistance to rice blast, bacterial leaf-blight, slice disease and 4 kinds of diseases of banded sclerotial blight; (good agricultural wxA1/good glutinous extensive No. 3) compares with former combination; Every mu can increase output 10%, reduces by 60 yuan of spraying pesticide costs.
Embodiment 3:
(1) be that glutinous recovery system is recurrent parent (with the R representative) good glutinous extensive No. 5 with three; With No. 1, rice blast resistant parent Chinese parasol tree farming (with the A representative), bacterial leaf-blight resistant parent CBB23 (with the B representative), the sick resistant parent Acc8558 of slice (with the C representative), banded sclerotial blight resistant parent Jasmine85 (with the D representative) hybridization, must hybridize F respectively
1
(2) plantation hybridization F
1Generation, F
2Generation, F
2For each combined grow 2000 strain; And its disease resistance identified that (each combination only identify the disease-resistant kind of its corresponding antibodies parent), each combination select high anti-individual plant 10 strains (screening agronomy is with yield traits and praise glutinous extensive No. 5 close individual plants) and recurrent parent to backcross; Plant the BC that backcrosses
1F
1Generation, BC
1F
2Generation, BC
1F
2Making up each strain for each is plantation 1000 strains, and its disease resistance is identified it is 2 that high anti-strain is selected in each combination, and each strain system selects high anti-individual plant 5 strains to backcross with good glutinous extensive No. 5, gets BC
2F
1Generation; Repeat above step to the 5 generation (BC that backcross
5F
1), plantation BC
5F
1, BC
5F
2, screening and obtaining agronomy is (respectively with F with yield traits with good glutinous extensive No. 5 glutinous recoveries similar, high anti-corresponding disease
A, F
B, F
C, F
DExpression);
(3) make hybrid combination 1 (F
A/ F
B), the combination 2 (F
C/ F
D), plantation hybridization F
1Generation, F
2Generation, F
2For each combined grow 2000 strain; And its disease resistance identified (each combination identifies that its corresponding antibodies parent resist 2 kinds of disease kinds); High anti-individual plant 5 strains (screening agronomy and yield traits and good glutinous extensive No. 5 close individual plants) and another combination biparental cross (combination 1/ combination 2), plantation hybridization F are selected in each combination
1Generation, F
2Generation, F
2Each plants 2000 strains generation 5 combinations; And its disease resistance identified (identifying that all parents resist 4 kinds of sick kinds); High anti-individual plant 5 strains (screening agronomy and yield traits and good glutinous extensive No. 5 close individual plants) are selected in each combination, and obtaining the extensively anti-glutinous recovery of 25 strains is strain;
(4) be that the strain kind becomes plant with the extensively anti-glutinous recovery of 25 strains, with good glutinous extensive No. 5 be contrast, to agronomy and yield traits article ratio, it is good to filter out economical character, the high strain of output is promptly bred wide anti-glutinous recovery system and " is extensively resisted and praise glutinous extensive No. 5 "." wide anti-good glutinous extensive No. 5 " not only can high simultaneously anti-4 kinds of diseases, other agronomy and yield traits with praise glutinous extensive No. 5 highly consistent, still possess high yield, high-quality characteristic.Wide anti-good glutinous extensive No. 5 waxy hybrid rices with good agricultural wxA1 preparation make up all has resistance to rice blast, bacterial leaf-blight, slice disease and 4 kinds of diseases of banded sclerotial blight; (good agricultural wxA1/good glutinous extensive No. 5) compares with former combination; Every mu can increase output 14%, reduces by 70 yuan of spraying pesticide costs.
The above is merely preferred embodiment of the present invention, and all equalizations of doing according to claim of the present invention change and modify, and all should belong to covering scope of the present invention.
Claims (3)
1. one kind wide anti-glutinous rice recovers the selection of system, and it is characterized in that: said selection may further comprise the steps:
(1) be that glutinous recovery is to be recurrent parent with three, with rice blast resistant parent-A, bacterial leaf-blight resistant parent-B, the sick resistant parent-C of slice, banded sclerotial blight resistant parent-D hybridization, totally four combinations must be hybridized F respectively
1
(2) plantation hybridization F
1Generation, F
2Generation, F
2Its disease resistance is identified that the disease-resistant kind of its corresponding antibodies parent is only identified in each combination for each combination, filtered out high anti-and agronomy individual plant and the recurrent parent close with the corresponding antibodies parent with yield traits backcrossed; Plant the BC that backcrosses
1F
1Generation, BC
1F
2Generation, BC
1F
2Make up each strain system for each its disease resistance is identified, the high anti-strain of screening system from each combination, and the high anti-individual plant of screening is backcrossed with recurrent parent from each high anti-strain system, gets BC
2F
1In generation, repeat above step to the BC that backcrosses
5F
1Generation, plantation BC
5F
1Generation, BC
5F
2In generation, screening at last obtains agronomy glutinous recovery similar with the corresponding antibodies parent with yield traits, that height resists corresponding disease and is, respectively with F
A, F
B, F
C, F
DExpression;
(3) make hybrid combination F
A/ F
B, F
C/ F
D, plantation hybridization F
1Generation, F
2Generation, F
2For each hybrid combination its disease resistance is identified; Identify that its corresponding hybrid antibody parent resists 2 kinds of sick kinds, the high anti-and agronomy individual plant biparental cross close with recurrent parent of each individual plant that hybrid combination choosing height is anti-and agronomy is close with recurrent parent with yield traits and another hybrid combination with yield traits; Plantation biparental cross F
1Generation, F
2Generation, F
2For each biparental cross combination its disease resistance is identified, identified 4 kinds of sick kinds that all parents resist, the high anti-and agronomy individual plant close with recurrent parent of each biparental cross combined sorting with yield traits, obtaining wide anti-glutinous rice recovery is strain;
(4) wide anti-glutinous rice being recovered is that the strain kind becomes plant, filters out the close and strain that yield traits is good of economical character and recurrent parent and breeds wide anti-glutinous recovery and be.
2. wide anti-glutinous rice according to claim 1 recovers the selection of system, it is characterized in that: with three is that glutinous recovery system is a recurrent parent good glutinous extensive No. 2, good glutinous extensive No. 3, good glutinous extensive No. 4 or good glutinous extensive No. 5.
3. wide anti-glutinous rice according to claim 1 recovers the selection of system; It is characterized in that: with the Chinese parasol tree farming is the rice blast resistant parent No. 1; With CBB23 is the bacterial leaf-blight resistant parent, is the sick resistant parent of slice with Acc8558, is the banded sclerotial blight resistant parent with Jasmine85.
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Cited By (8)
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| CN103329796A (en) * | 2013-07-11 | 2013-10-02 | 江西省农业科学院水稻研究所 | General method for rapidly and directionally cultivating novel rice cytoplasm male sterile restoration source |
| CN103931490A (en) * | 2014-04-04 | 2014-07-23 | 福建农林大学 | Breeding method of resistance-variable rice variety |
| CN103931484A (en) * | 2014-04-04 | 2014-07-23 | 福建农林大学 | Breeding method of widely-resistive wheat variety |
| CN104082124A (en) * | 2014-06-30 | 2014-10-08 | 章清杞 | Selective breeding method of high-resistance tobacco variety |
| CN104137766A (en) * | 2014-07-14 | 2014-11-12 | 福建农林大学 | Breeding method of special type giant embryo rice restorer |
| CN105724242A (en) * | 2016-03-09 | 2016-07-06 | 福建农林大学 | Breeding method of upland rice variety for feed |
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| CN105746336A (en) * | 2016-03-09 | 2016-07-13 | 福建农林大学 | Method for breeding three-line sterile line of silage upland rice |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103329796B (en) * | 2013-07-11 | 2016-03-02 | 江西省农业科学院水稻研究所 | The universal method of paddy rice cytoplasmic male sterilty restorer is cultivated in a kind of slewing |
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| CN103931490A (en) * | 2014-04-04 | 2014-07-23 | 福建农林大学 | Breeding method of resistance-variable rice variety |
| CN103931484A (en) * | 2014-04-04 | 2014-07-23 | 福建农林大学 | Breeding method of widely-resistive wheat variety |
| CN103931484B (en) * | 2014-04-04 | 2016-05-11 | 福建农林大学 | A kind of selection of wide anti-wheat breed |
| CN104082124A (en) * | 2014-06-30 | 2014-10-08 | 章清杞 | Selective breeding method of high-resistance tobacco variety |
| CN104082124B (en) * | 2014-06-30 | 2016-08-24 | 福建农林大学 | A kind of selection of resistance tobacco bred |
| CN104137766A (en) * | 2014-07-14 | 2014-11-12 | 福建农林大学 | Breeding method of special type giant embryo rice restorer |
| CN105724242A (en) * | 2016-03-09 | 2016-07-06 | 福建农林大学 | Breeding method of upland rice variety for feed |
| CN105746337A (en) * | 2016-03-09 | 2016-07-13 | 福建农林大学 | Method for breeding silage upland rice variety |
| CN105746336A (en) * | 2016-03-09 | 2016-07-13 | 福建农林大学 | Method for breeding three-line sterile line of silage upland rice |
| CN105746336B (en) * | 2016-03-09 | 2017-09-15 | 福建农林大学 | A kind of selection of ensilage upland rice CMS line |
| CN105746337B (en) * | 2016-03-09 | 2017-09-15 | 福建农林大学 | A kind of selection of ensilage upland rice variety |
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