CN103329796B

CN103329796B - The universal method of paddy rice cytoplasmic male sterilty restorer is cultivated in a kind of slewing

Info

Publication number: CN103329796B
Application number: CN201310289838.2A
Authority: CN
Inventors: 沈显华; 黄仁良; 严松; 沈林军; 熊宏亮; 朱珊
Original assignee: RICE RESEARCH INSTITUTE OF JIANGXI ACADEMY OF AGRICULTURAL SCIENCES
Current assignee: RICE RESEARCH INSTITUTE OF JIANGXI ACADEMY OF AGRICULTURAL SCIENCES
Priority date: 2013-07-11
Filing date: 2013-07-11
Publication date: 2016-03-02
Anticipated expiration: 2033-07-11
Also published as: CN103329796A

Abstract

The invention discloses the universal method that paddy rice cytoplasmic male sterilty restorer is cultivated in a kind of slewing, with paddy rice cytoplasm male sterility line for female parent, with the primary fine kytoplasm source donor material of this cytoplasm male sterility line for male parent, carry out hybridization and obtain F ₁; With existing rice varieties for the male parent that backcrosses, with above-mentioned F ₁reestablish diplomatic relations; Afterwards, continuous backcross 2 ~ 3 times; When backcrossing, the individual plant all selecting pollen normally can educate from backcross population, for maternal, take backcross parent as male parent, backcrosses; At BC _3-4f ₁in colony, select the individual plant bagging selfing that pollen normally can be educated, obtain BC _{3 ~ 4}f ₂; At all BC _{3 ~ 4}f ₂in strain, screen the strain that pollen normally can be educated, Comprehensive Traits is similar to backcross parent.By technical method provided by the present invention, the supporting application of novel cytoplasmic male sterilty paddy rice " three are " can be accelerated, reproducible, reliable results.

Description

The universal method of paddy rice cytoplasmic male sterilty restorer is cultivated in a kind of slewing

Technical field

The invention belongs to technique for breeding of hybridized rice field, cultivate the universal method of paddy rice cytoplasmic male sterilty restorer in particular to a kind of slewing.

Background technology

Since 20 century 70s, by paddy rice interspecific hybridization and the seed selection of nucleus substitution method rice cytosolic male (CMS) male sterile line of multiple separate sources.At present, the separate sources rice cytosolic male sterile line of application in production, the male sterile cytoplasm type that, guarantor relation extensive according to it totally can be divided into wild abortion type (WA-CMS), bag bench-type (BT-CMS) and red lotus type (HL-CMS) etc. 3 kinds main.

Continuous cultivation has the cytoplasm male sterility line in Cytoplasmic male sterility source and the restorer in Restore gene source thereof, being the effective technology means avoiding hybrid rice to produce potential risk owing to promoting single cell matter source for a long time, is also overcome in current rice cytosolic male sterile line, Breeding for restoration lines process because the existing Rice Germplasm Resources brought by extensive, the restriction of guarantor's relation special validity utilizes the effective way mutually limiting this drawback.

After the cytoplasm male sterility line cultivating novel fine kytoplasm source, find its corresponding cytoplasmic male sterilty restorer, realizing " three are " (male sterile line, maintainer and restorer) supporting, is the key issue that breeder must solve.The most frequently used solution be utilize existing all kinds of restorer kind (restorer of WA-CMS, HL-CMS, BT-CMS) and maintainer and other rice varieties carry out extensive testcross and screening restorer or can be extensive kind matter, this method is commonly called as " extensively survey extensive method ".But, in the process that new germplasm source cytoplasm male sterility line is cultivated, usually run into by extensively surveying extensive method and restorer or Restorer varieties can not be screened.As, perennial wild rice that International Rice utilizes (female parent) and cultivated rice (hybridizing and the male parent that backcrosses) carry out hybridizing, backcrossing, cultivate the novel cytoplasm male sterility line IR66707A from perennial wild rice cytoplasmic male sterilty matter source, Jiaxing City Academy of Agricultural Sciences of China is sterile with this is maternal, cultivate good early 935A, the male sterile line in this novel cytoplasmic male sterilty matter source, do not find the Restorer varieties that restoring degree is good yet till now, do not cultivate strong advantage restorer yet.

Summary of the invention

The object of this invention is to provide the universal method that paddy rice cytoplasmic male sterilty restorer is cultivated in a kind of slewing.In order to realize object of the present invention, intend adopting following technical scheme:

One aspect of the present invention relates to the universal method of cultivating rice cytosolic male sterile restoring line, and it comprises the steps: to take cytoplasm male sterility line as female parent, with the primary fine kytoplasm source donor material of this cytoplasm male sterility line for male parent, carries out hybridization and obtains F ₁; With the rice varieties that existing flowering habit is good, pollen amount is large, Comprehensive Traits is excellent, coordinate force is good (such as: the restorer of existing hybrid rice) for the male parent that backcrosses, with above-mentioned F ₁reestablish diplomatic relations; At the F that reestablishes diplomatic relations ₁in, the individual plant normally can educated with pollen is female parent, then backcrosses with the above-mentioned male parent that backcrosses, and obtains BC ₁f ₁; Afterwards, continuous backcross 2 ~ 3 times; When backcrossing, from backcross population, all select that pollen normally can be educated at every turn, phenotype for maternal like the individual plant of backcross parent, take backcross parent as male parent, backcross; At BC _3-4f ₁in colony, select that pollen normally can be educated, phenotype like the individual plant bagging selfing of backcross parent, obtain BC _{3 ~ 4}f ₂; At all BC _{3 ~ 4}f ₂in strain, screening pollen normally can educate (namely pollen fertility is not separated), the Comprehensive Traits strain similar to backcross parent; Middle roguing system is the restorer of this cytoplasmic male sterilty

In a preferred embodiment of the present invention, described cytoplasm male sterility line still screens paddy rice cytoplasm male sterility line less than the good Restorer varieties of restoring degree by " extensively survey extensive method ".

In another preferred embodiment of the present invention, the cytoplasm male sterility line that described cytoplasm male sterility line is Dongxiang Wild Rice is kytoplasm, middle morning 35 is cell nucleus background, primary fine kytoplasm source donor material is Dongxiang Wild Rice.

By technical method provided by the present invention, the supporting application of novel cytoplasmic male sterilty paddy rice " three are " can be accelerated, reproducible, reliable results.

Accompanying drawing illustrates:

Fig. 1: the novel cytoplasm male sterility line in Dongxiang Wild Rice kytoplasm source---middle 35A seed selection morning flow chart.

Fig. 2: the novel cytoplasm male sterility line in Dongxiang Wild Rice kytoplasm source---middle morning 35A restorer quick breeding flow chart.

Embodiment

By Dongxiang Wild Rice (male normally can educate) for maternal, Early-season indica rice kind " middle morning 35 " is the male parent that backcrosses, backcross through 6 generations, to obtain with Dongxiang Wild Rice be kytoplasm, new germplasm source cytoplasm male sterility line (see Fig. 1) that middle morning 35 is cell nucleus background.

In order to determine that this new germplasm source is guaranteed the quality the Rescued virus of male sterile line, we utilize existing " wild abortion type ", the maintainer of " red lotus type " hybrid rice and restorer and conventional rice kind, respectively with early 35 test crosses in this new germplasm source cytoplasm male sterility line.The all test cross F of result ₁be all male sterile, and sterile degree very thoroughly (table 1).Illustrate: the first, the maintainer of existing " wild abortion type ", " red lotus type " hybrid rice and restorer, have retentivity thoroughly to the male sterility of this new germplasm source cytoplasm male sterility line; Second, the Rescued virus of this new germplasm source cytoplasm male sterility line is different from " wild abortion type " and " red lotus type " completely, belong to novel cytoplasm male sterility line, because no matter be " wild abortion type " or " red lotus type ", its restorer and maintainer all novelly sterilely can not have recovery capability to this; 3rd, conventional variety (in table 1 last 7) can not have recovery capability to this novel male sterile line, on the contrary, has hold facility thoroughly.

Early 35A and Tester test cross F in the male sterile line in table 1 Dongxiang Wild Rice kytoplasm source ₁can dyeing flower powder rate

¹: in bracket, WAR and WAB represents restorer, the maintainer of wild abortion type respectively, HLR and HLB represents restorer, the maintainer of red lotus type respectively.

Early 35A and Dongxiang Wild Rice test cross F in the male sterile line in table 2 Dongxiang Wild Rice kytoplasm source ₁can dyeing flower powder rate and ripening rate

With this novel cytoplasm male sterility line for female parent, with the primary fine kytoplasm source donor material of this novel cytoplasm male sterility line---" Dongxiang Wild Rice ", for male parent, is carried out hybridization and is obtained F ₁.Then, with the rice varieties that existing flowering habit is good, pollen amount is large, Comprehensive Traits is excellent, coordinate force is good---" R463 (existing wild abortion type hybrid early restorer) " (backcross parent), for male parent, carries out hybridization acquisition and to reestablish diplomatic relations F ₁.At this F that reestablishes diplomatic relations ₁in colony, during heading, select that fringe neck degree of stretching out is good, the normal individual plant of pollen fertility 10, backcross with " R463 ", obtain BC ₁f ₁strain 10.At BC ₁f ₁in strain, select that fringe neck degree of stretching out is good, pollen fertility normal altogether, phenotype like the individual plant 50 of " R463 ", backcross with " R463 ", obtain BC ₂f ₁strain 50.At BC ₂f ₁in strain, select that fringe neck degree of stretching out is good, pollen fertility normal altogether, phenotype like the individual plant 50 of " R463 ", backcross with " R463 ", obtain BC ₃f ₁strain 50.At BC ₃f ₁in strain, select that fringe neck degree of stretching out is good, pollen fertility normal altogether, phenotype like the individual plant 592 of " R463 ", bagging selfing, obtains BC ₃f ₂strain 592.At BC ₃f ₂in strain, selection fringe neck degree of stretching out is good, pollen fertility normal (in strain, all individual plant pollen fertilities are all normal), phenotype carry out selfing like the strain 8 of " R463 ", obtains BC ₃f ₃strain.Concrete Breeding Process is shown in Fig. 2.Meanwhile, we are with 8 of middle choosing BC ₃f ₂strain, with early 35A test cross combo in this novel cytoplasm male sterility line.8 F ₁all show as normal pollen development and ripening rate.Show, middle roguing system is exactly the restorer of this novel cytoplasm male sterility line.

The above is the preferred embodiments of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from principle of the present invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.

Claims

1. the breeding method of the novel cytoplasmic male sterilty restorer in a Dongxiang Wild Rice kytoplasm source, it comprises the steps: with rice cytosolic male sterile is maternal, with the primary fine kytoplasm source donor material of this cytoplasm male sterility line for male parent, carry out hybridization and obtain F ₁; With the rice varieties that existing flowering habit is good, pollen amount is large, Comprehensive Traits is excellent, coordinate force is good for the male parent that backcrosses, with above-mentioned F ₁reestablish diplomatic relations; At the F that reestablishes diplomatic relations ₁in, the individual plant normally can educated with pollen is female parent, then backcrosses with the above-mentioned male parent that backcrosses, and obtains BC ₁f ₁; Afterwards, continuous backcross 2 ~ 3 times; When backcrossing, from backcross population, all select that pollen normally can be educated at every turn, phenotype for maternal like the individual plant of backcross parent, take backcross parent as male parent, backcross; At BC _{3 ~ 4}f ₁in colony, select that pollen normally can be educated, phenotype like the individual plant bagging selfing of backcross parent, obtain BC _{3 ~ 4}f ₂; At all BC _{3 ~ 4}f ₂in strain, screen the strain that pollen normally can be educated, Comprehensive Traits is similar to backcross parent; Middle roguing system is the restorer of this cytoplasmic male sterilty; The cytoplasm male sterility line that described cytoplasm male sterility line is Dongxiang Wild Rice is kytoplasm, middle morning 35 is cell nucleus background, primary fine kytoplasm source donor material is Dongxiang Wild Rice.

2. the breeding method of the novel cytoplasmic male sterilty restorer in Dongxiang Wild Rice kytoplasm source according to claim 1, described rice cytosolic male sterile line still screens paddy rice cytoplasm male sterility line less than the good Restorer varieties of restoring degree by " extensively surveying extensive method ".