CN107372100B - A kind of method of Sorghum Hybrid breeding - Google Patents

A kind of method of Sorghum Hybrid breeding Download PDF

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Publication number
CN107372100B
CN107372100B CN201710760447.2A CN201710760447A CN107372100B CN 107372100 B CN107372100 B CN 107372100B CN 201710760447 A CN201710760447 A CN 201710760447A CN 107372100 B CN107372100 B CN 107372100B
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sorghum
parent
seed
male
female
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CN107372100A (en
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龙文靖
倪先林
刘天朋
赵甘霖
汪小楷
丁国祥
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RICE AND SORGHUM INSTITUTE SICHUAN ACADEMY OF AGRICULTURAL SCIENCES
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RICE AND SORGHUM INSTITUTE SICHUAN ACADEMY OF AGRICULTURAL SCIENCES
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a kind of method of Sorghum Hybrid breeding, include the following steps: that October and mid-October sow sorghum material respectively, makes sorghum booting florescence Daily minimum temperature respectively in 15-20 DEG C and 10-15 DEG C;Selecting equal male sterility under sowing condition twice, the fertile material of female is female parent, and male fertile material is male parent under sowing condition twice;Advantageous combination is carried out to the female parent material and male parent material screened, first familiar generation seed is obtained, F1 seed is sowed, remove F1 generation false hybrid, and bagging is selfed to obtain F2 for seed, selects strain bagging selfing consistent with objective trait to get ideal self-mating system from offspring.The present invention can be hybridized using restorer, holding system, cenospecies, conventional kind of male sterility under the conditions of special climate with conventional kind, restorer, holding system, cenospecies, reduce artificial emasculation workload, and guarantee hybrid seed quantity, it is expected to select the Parents In Sorghum material of high yield and high quality better resistance.

Description

A kind of method of Sorghum Hybrid breeding
Technical field
The present invention relates to genetic breeding fields, and in particular to a kind of method of Sorghum Hybrid breeding.
Background technique
Sorghum [Sorghum bicolor (L.) Moench] is one of important cereal crop of China with drought-enduring, resistance to The multiple resistances such as flood, impoverishment tolerant, saline-alkali tolerant, Plain, massif, salt-soda soil can plant, and have high biological yield and economy Yield.Sorghum is mainly to carry out heterosis utilization with " three systems " system, and the selection of Parents system is apolegamy superior hybrid crosses Combined key.Parental department is mainly to pass through crossbreeding breeding at present, in Breeding Process, artificial emasculation heavy workload, and behaviour Make cumbersome, low efficiency, and hybrid seed amount is few.
Summary of the invention
To solve the above problems, can be by restorer, holding the present invention provides a kind of method of Sorghum Hybrid breeding, Conventional kind, cenospecies be converted into infertility with a large amount of restorers, keep system or routine sorghum material hybridize, it is expected to it is excellent to select high yield The Parents In Sorghum material of matter better resistance.
To achieve the above object, the technical scheme adopted by the invention is as follows:
A kind of method of Sorghum Hybrid breeding, includes the following steps:
S1, October and mid-October sow sorghum material in Yuanjiang County of Yunnan respectively, are selected according to different materials breeding time The specific sowing time, so that the sorghum florescence minimum temperature sowed twice is respectively in 15-20 DEG C and 10-15 DEG C;It will broadcast twice Male sterility, the fertile material of female be as maternal under the conditions of kind, will male fertile material is made under sowing condition twice For male parent;
S2, advantageous combination is carried out to the female parent material and male parent material screened
According to breeding objective, the Parent material with objective trait is selected, is sowed second year October in Yuanjiang County of Yunnan, And make the Parent florescence consistent according to adjustment date of seeding Parent breeding time;Then bagging processing is carried out to female parent, in Post flowering Male parent material pollen is taken to pollinate, to obtain first familiar generation seed;
S3, filial generation is cultivated and is selected
Resulting F1 generation seed is sowed, removes F1 generation false hybrid, and bagging is selfed to obtain F2 for seed, from F2 for seed Offspring in the consistent strain bagging of selection target character be selfed to get ideal self-mating system.
Breeding method of the invention can be using restorer, holding system, cenospecies, conventional kind of male under the conditions of special climate not It educates and hybridizes with conventional kind, restorer, holding system, cenospecies, reduce artificial emasculation workload, and guarantee hybrid seed quantity.
Specific embodiment
In order to which objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further It is described in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair It is bright.
The applicant has found that sorghum, which encounters relative low temperature then in florescence, can cause sorghum male in long-term sorghum is studied Infertility, and there are apparent genotypic differences between different self-mating systems.It can be in special ecological point by fertility using genotypic difference The higher self-mating system of inversion temperature does female parent, and the lower self-mating system of Fertility Transfer Temperature does male parent and carries out advantage hybridization, thus It selects and meets breeding objective self-mating system, specifically, including the following steps:
S1, existing sorghum material is screened
October and mid-October sow sorghum material in Yuanjiang County of Yunnan respectively, are selected according to different materials breeding time specific Sowing time, so that the sorghum florescence minimum temperature sowed twice is respectively in 15-20 DEG C and 10-15 DEG C;Item will be sowed twice Equal male sterility under part, the fertile material of female as maternal, will twice under sowing condition male fertile material as father This;
S2, advantageous combination is carried out to the female parent material and male parent material screened
According to breeding objective, the Parent material with objective trait is selected, is sowed second year October in Yuanjiang County of Yunnan, And make the Parent florescence consistent according to adjustment date of seeding Parent breeding time;Then bagging processing is carried out to female parent, in Post flowering Male parent material pollen is taken to pollinate, to obtain first familiar generation seed;
S3, filial generation is cultivated and is selected
Resulting F1 generation seed is sowed, removes F1 generation false hybrid, and bagging is selfed to obtain F2 for seed, from F2 for seed Offspring in the consistent strain bagging of selection target character be selfed to get ideal self-mating system.
Embodiment 1
S1, existing sorghum material is screened
October and mid-October sow sorghum material in Yuanjiang County of Yunnan respectively, are selected according to different materials breeding time specific Sowing time makes sorghum florescence Daily minimum temperature respectively in 15-20 DEG C and 10-15 DEG C;It selects male under sowing condition twice Property infertility material be female parent, twice under sowing condition normal material be male parent;
S2, advantageous combination is carried out to the female parent material and male parent material screened
According to breeding objective, male parent material 405B and the preferable female parent material of yield traits with certain short source are selected 45B continues to sow in Yuanjiang County of Yunnan, and makes the Parent florescence one according to adjustment date of seeding Parent breeding time second year October It causes.Carry out bagging to 45B takes 405B material pollen to pollinate in Post flowering, to obtain first familiar generation seed.
S3, filial generation is cultivated and is selected
F1 seed is sowed, removes F1 generation false hybrid, and bagging is selfed to obtain F2 for seed, and strain is constantly selected from offspring Blade profile is good, and the higher strain bagging selfing of yield finally obtains ideal self-mating system 1509B.
Embodiment 2
S1, existing sorghum material is screened
October and mid-October sow sorghum material in Yuanjiang County of Yunnan respectively, are selected according to different materials breeding time specific Sowing time makes sorghum florescence Daily minimum temperature respectively in 15-20 DEG C and 10-15 DEG C;It selects male under sowing condition twice Property infertility material be female parent, twice under sowing condition normal material be male parent;
S2, advantageous combination is carried out to the female parent material and male parent material screened
According to breeding objective, male parent material 272R with certain short source and breeding time female parent material earlier are selected 1609A × 186R, Yu Dangnian November are sowed in Hainan Lingshui base, and make Parent according to adjustment date of seeding Parent breeding time Florescence is consistent.Carry out bagging to cenospecies 1609A × 186R takes 272R material pollen to pollinate in Post flowering, to obtain 09A × 186R × 272R triple hybrid;
S3, filial generation is cultivated and is selected
To strain bagging selfing consistent with objective trait is constantly selected in triple hybrid offspring, ideal selfing is finally obtained System.
The above is only presently preferred embodiments of the present invention, it is noted that for those skilled in the art For, without departing from the principle of the present invention, it can also make several improvements and retouch, these improvements and modifications should also regard For protection scope of the present invention.

Claims (1)

1. a kind of method of Sorghum Hybrid breeding, which comprises the steps of:
S1, October and mid-October sow sorghum material in Yuanjiang County of Yunnan respectively, so that the sorghum florescence sowed twice is most Low temperature is respectively in 15-20 DEG C and 10-15 DEG C;Equal male sterility under sowing condition twice, the fertile material of female are incited somebody to action as female This, will twice under sowing condition male fertile material as male parent;
S2, advantageous combination is carried out to the female parent material and male parent material screened
According to breeding objective, the Parent material with objective trait is selected, is sowed second year October in Yuanjiang County of Yunnan, and root Make the Parent florescence consistent according to adjustment date of seeding Parent breeding time;Then bagging processing is carried out to female parent, takes father in Post flowering This material pollen is pollinated, to obtain first familiar generation seed;
S3, filial generation is cultivated and is selected
Resulting F1 generation seed is sowed, removes F1 generation false hybrid, and bagging is selfed to obtain F2 for seed, from F2 for seed after The consistent strain bagging selfing of selection target character is in generation to get ideal self-mating system.
CN201710760447.2A 2017-08-23 2017-08-23 A kind of method of Sorghum Hybrid breeding Active CN107372100B (en)

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108739349A (en) * 2018-03-13 2018-11-06 四川省农业科学院水稻高粱研究所 A kind of selection of sorghum restorer line
CN112753571A (en) * 2021-03-15 2021-05-07 四川省农业科学院水稻高粱研究所 Breeding method of sorghum cytoplasmic male sterile line with high tannin content

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1283385A (en) * 2000-09-15 2001-02-14 朱永峰 Method fro selective cultivation, reproduction and seed preparation of hydridized sorghum with new combination
CN102835301A (en) * 2012-02-01 2012-12-26 吉林省农业科学院 Breeding, seed-producing and cultivating method for sorghum coeno-species Jiza 130

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1102335C (en) * 1999-06-03 2003-03-05 湖南省农业科学院高粱研究开发中心 Two-line hybridization method for producing sorghum seeds
CN101288380A (en) * 2008-03-19 2008-10-22 广东省农科集团良种苗木中心 Good quality, high yield, disease-resistant and wide adaptation ultra-sweet corn breeding method
CN103843555A (en) * 2013-08-27 2014-06-11 肖卓丽 High-yield and purity-maintaining seed production technique of two-line hybrid rice

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1283385A (en) * 2000-09-15 2001-02-14 朱永峰 Method fro selective cultivation, reproduction and seed preparation of hydridized sorghum with new combination
CN102835301A (en) * 2012-02-01 2012-12-26 吉林省农业科学院 Breeding, seed-producing and cultivating method for sorghum coeno-species Jiza 130

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