CN103843555A - High-yield and purity-maintaining seed production technique of two-line hybrid rice - Google Patents

High-yield and purity-maintaining seed production technique of two-line hybrid rice Download PDF

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CN103843555A
CN103843555A CN201310375565.3A CN201310375565A CN103843555A CN 103843555 A CN103843555 A CN 103843555A CN 201310375565 A CN201310375565 A CN 201310375565A CN 103843555 A CN103843555 A CN 103843555A
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temperature
rice
greenhouse
sterile
seed production
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肖卓丽
肖小林
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Abstract

The invention belongs to the technical field of rice seed production, and particularly relates to a purity-maintaining and high-yield seed production method in the field of seed production of two-line hybrid rice. The method is technically characterized in that a greenhouse or arched greenhouse technique is adopted in the middle and later periods of seed production of two-line hybrid rice. The method specifically comprises the following step that when the adverse environment conditions, such as overlong or overshort lighting, overhigh or overlow air temperature and the like, are encountered or possibly encountered in one certain growth phase of the later period of the seed production of the two-line hybrid rice, the environment is controlled by the greenhouse or the arched greenhouse. Compared with the background art, the method successfully solves the problems of easy influence on fertility restoration due to the adverse air temperature factors during the light and temperature induced sterility transformation sensitive period for the seed production of the light and temperature sensitive genic male sterility rice, and the physiological disorder and physiological injury to male and female parents due to abnormal high temperature or low temperature weathers.

Description

A kind of high yield of double-linear hybrid rice, pure keeping seeding technique
Technical field
The invention belongs to paddy rice seeding technique field, is pure keeping, the high-yield seeds production technique of two-line hybrid rice.More definite, be the technology of " optical-and thermal-sensitive male nuclear sterile rice intermediary and later stages application greenhouse, booth or shed technology are carried out the production of hybrid seeds ".
Background technology
1. optical-and thermal-sensitive male nuclear sterile rice and greenhouse, booth and the brief introduction of shed application technology
1.1 optical-and thermal-sensitive male nuclear sterile rice brief introductions
Optical-and thermal-sensitive male nuclear sterile rice is a kind of " gene and environment are made male sterile type mutually " paddy rice, is called again " ecological genotype nuclear sterile rice ", is found first in 1972 by China scientist Shi Mingsong.Its sterile generation had both been subject to gene control, affected by illumination and temperature, therefore the title of " optical-and thermal-sensitive male nuclear sterile rice " is more definite to it.
Illumination and temperature affect period of optical-and thermal-sensitive male nuclear sterile rice fertility conversion can because of optical-and thermal-sensitive male nuclear sterile rice, to produce the difference of sterile morphosis genesis mechanism slightly variant, generally speaking, certain stage of the 5-20 before Rice Flowering days is the sensitive periods of induction optical-and thermal-sensitive male nuclear sterile rice fertility conversion.Paddy rice is sterile following morphosis genesis mechanism conventionally: stamen development is complete, flower pesticide shrinkage, microspore development are obstructed, flower pesticide does not ftracture, pollen grain is not sprouted etc.
Various optical-and thermal-sensitive male nuclear sterile rices, also have the illumination of its induction fertility conversion separately and the critical value of temperature.
People are the response characteristic to illumination and temperature according to the fertility of optical-and thermal-sensitive male nuclear sterile rice, and optical-and thermal-sensitive male nuclear sterile rice is divided into: 1 Photosensitive, 2 temp.-sensitive types, 3 interaction by light and temperature responsive type three types.Wherein, Photosensitive can be further divided into: within 1 long day sterile-short day, can educate type; Within 2 long days, can educate-short day sterile type.Temp.-sensitive type can be further divided into: 1 high temperature-induced sterility-low temperature can be educated type; The sterile type of can educate-low temperature of 2 high temperature; 3 higher than certain thermal extremes or sterile lower than certain extreme low temperature, between between two extreme temperatures, can educate type; Beyond 4 liang of extreme temperatures, can educate, two extreme between the sterile type of temperature.Interaction by light and temperature type is the main Types of optical-and thermal-sensitive male nuclear sterile rice, and the optical-and thermal-sensitive male nuclear sterile rice of finding at present belongs to this type of more, roughly can be further divided into the leading or leading illumination temperature of temperature of illumination and jointly promote type.Segment more complicatedly, as: (1) Nongken 58S, to belong to the long-day leading sterile, and high temperature promotes sterile; Short-day is leading can educate, and low temperature promotes to educate type.(2) to belong to high temperature leading sterile for W6154S, within long day, promotes sterile; Low temperature is leading can educate, and within short day, promotes to educate type.(3) to belong to short illumination leading sterile for D38S, and low temperature promotes sterile; Long illumination is leading can educate, and high temperature promotes to educate type.For most interaction by light and temperature type optical-and thermal-sensitive male sterility line, its fertility, for the relation between illumination and temperature, can be described [1] with a three-dimension curved surface.
A core value of optical-and thermal-sensitive male nuclear sterile rice can be carried out with it exactly " two is method " and be produced hybrid rice, alleged " two is method " " two is method " that all refer to if no special instructions to apply optical-and thermal-sensitive male nuclear sterile rice and carry out the production of hybrid seeds that (comprise " summary " and " claims ") below, described " male sterile line " refers to optical-and thermal-sensitive male sterility line if no special instructions." two is method " produces hybrid rice than " three line method " (utilization of matter-core interaction type male sterile line), has technically significant progressive, is mainly manifested in: 1) combo is more free.There is not specific extensive guarantor's relation to optical-and thermal-sensitive male nuclear sterile rice in most kinds, can recover its fertility.This has just increased the probability that selects desirable combination; 2) male sterile line can self propagated, without maintainer, makes breeding technique easier.Just because of this, the research of optical-and thermal-sensitive male nuclear sterile rice utilization is put into National 863 planning item.But; utilize the defect of optical-and thermal-sensitive male nuclear sterile rice production hybrid rice also very outstanding; wherein the most serious defect is: male sterile line is when for the production of hybrid seeds; in the sensitive periods of its fertility conversion; often can meet the too high or too low temperature that anomalous weather brings, cause its fertility repeatedly and part self-fertility.The hybrid seed that this class is mixed with selfed seed can bring the underproduction, on producing, can not use.Regrettably, up to now two be that seeding technique can't solve this technical barrier effectively, it is still so very long that this just makes " two is method " large area enter the distance of practical stage.
1.2 greenhouses, booth and the brief introduction of shed application technology
Greenhouse, booth or shed are that one can change plant growth environment, create optimal environmental condition for plant growth, can avoid, prevent and alleviate the horticultural facility of the environmental influence crop growths such as terrible weather factor.It is using daylighting cladding material as all or part of structural material, can be in the winter time or other be not suitable for the cultivated plant in season of open country plant growth.The key technology of greenhouse, booth or shed is the control of environment control, especially illumination and temperature.
The daylighting cladding material of greenhouse, booth is mainly glass, lucite and plastic foil, and the type of its structure and its function is varied.Some greenhouse and booth can also be assemblings, split or movable type.The method of greenhouse and booth controlled light and temperature mainly contains: 1) under low-temperature condition, require to heat up: first with the lighting material covering farm land of greenhouse or booth, form the environment of a relative closure, so just, can reduce circulation of air dissipated heat to atmosphere and reduce longwave optical radiation and be convenient to insulation, then, obtain sunlight by lighting material again and heat, and (or) by other Heating systems heat (as electricity heating or coal stove heating etc.).2) under the condition of high temperature, require cooling: first with greenhouse or booth, farmland is covered, cover again sunshade net (or Sunshade film),, then use cooling installation (as heat pump) and method (as taken off lid at dusk, take off film, opening the door, window, filling with daytime such as cold water, spray cold water etc.) to lower the temperature then.Involving great expense of greenhouse and booth, but they are better to the control effect of light, warm key element, especially intelligent greenhouse, booth, can also realize automation and the precision of environment control.
Shed is mainly made up of support and plastic foil.Because of most sheds cooling system of not heating, regulating the effect that often can only play insulation aspect temperature.In practice, bring into play it and " heat " or " cooling " effect, mainly the different moment lid by fine day, take off film and reach the effect that adds, lowers the temperature.To overcast and rainy for a long time, these do not add, the shed of cooling system does not have the effect that adds, lowers the temperature.Shed is with respect to greenhouse and booth, and its advantage is low cost, and demolition, installation are convenient, simply adds cooling system and method if can add some temporarily, also can reach certain and adds, cooling-down effect.
Since the nineties in last century, the technical development of greenhouse, booth and shed is rapid, and usable floor area also expands rapidly.Especially vegetables in improper season greenhouse, booth are in full flourish.This makes greenhouse, booth and shed technology ripe and perfect day by day, and is close to the feature that becomes modern agriculture with high efficiency.
2. the background technology of " two be method " production of hybrid seeds corresponding with the present invention
Up to now, it is the production of hybrid seeds that application optical-and thermal-sensitive male nuclear sterile rice implements two, except seeding stage can use film, shed, booth or greenhouse, any one growing stage is after this all to carry out under natural environmental condition in the open, without any insulation, the facility of temperature control.
This outdoor seeding technique can not effectively be controlled the environmental condition of seed farm especially temperature condition, and it is special, two is that the father of the production of hybrid seeds, maternal some growing stage of its middle and later periods are particularly harsh to the requirement of temperature, if temperature fails to meet the demands by generation serious consequence.Because existing seeding technique can not effectively manipulate the environmental condition such as temperature, illumination of seed farm, make two to be some problems that must solve and fail to obtain effectively and thoroughly to solve in the production of hybrid seeds.Attempting several routine this respect problems below understands for everybody:
1, existing two is that seeding technique is failed thoroughly to solve in the production of hybrid seeds of optical-and thermal-sensitive male nuclear sterile rice it always and within sensitive periods of light temperature induction fertility conversion, is subject to unfavorable Temperature Factor impact and the problem that causes fertility restorer.
Owing to can not effectively controlling temperature and the illumination of seed farm, existing two be seeding technique overriding concern be a bit (to be generally about 5-20 days before heading the sensitive periods of induction optical-and thermal-sensitive male nuclear sterile rice fertility conversion, slightly variant between different sterile type male sterile lines) be arranged in suitable season or the date section of suitable region, make illumination and the temperature of this season or date section can not exceed this male sterile line from the sterile fertility conversion critical value that can educate that is converted to, to reaching the object that makes this male sterile line continue to keep highly sterile state.For example: when the sterile lines seed making that can educate at sterile-low temperature of application high temperature leading (or promotion) leading (or promotion), bring the risk in hybrid seed production of fertility restorer to male sterile line for avoiding the appearance of abnormal low temperature weather, often the sensitive periods of induction male sterile line fertility conversion, is chosen in to the season (or period) that in 1 year, temperature is the highest, in annual 7 months, the last ten-days period and early August; The selection of region is that latitude and height above sea level all can not be too high, and general latitude is no more than north latitude 40 and spends, and height above sea level is no more than 1800 meters; To induce the temperature threshold value of its fertility conversion low as far as possible to the selection of male sterile line, should not exceed 27 degrees Celsius, because most rice workspace difficulties look for one can definitely ensure that 10 days left and right temperature is stable and exceed the date section of 27 degrees Celsius, its fraction is many below 80%.In fact;, high temperature leading sterile long day of application promotes leadingly can educate for sterile-short day, low temperature promotes to educate type; or application high temperature within leading sterile, long day, promote sterile-low temperature leadingly can educate, short day while promoting to educate type sterile lines seed making; even these class male sterile lines induce the temperature threshold value of its fertility conversion close to the physiology lower limit (being generally 21 DEG C) of this male sterile line; in the sensitive periods of its fertility conversion, also often can meet lower than or close to the temperature of 21 DEG C.Again for example, can educate at sterile-high temperature of application of cold temperature leading (or promotion) leading (or promotion) sterile lines seed making time, bring the risk in hybrid seed production of fertility restorer to male sterile line for avoiding the appearance of abnormal high temperature weather, first must consider the fertility of male sterile line to be arranged in early summer or season in late fall to the sensitive periods of light temperature reaction, the temperature threshold value of its fertility conversion of the induction of male sterile line is higher as far as possible.But, even the type male sterile line induces the temperature of its fertility conversion to reach 35 DEG C, also can cause to paddy rice the temperature of heat evil, and in early summer and late fall, it is also common that temperature exceedes 35 DEG C, will cause male sterile line fertility repeatedly like that.Therefore use the risk of this class sterile lines seed making also very high.And if will the sowing season of this type of male sterile line in advance or postpone, the front or rear phase of its breeding time, will meet with again the harm of low temperature.
2, existing two is that seeding technique fails to solve physiological disorder that abnormal high temperature or microthermal climate bring to father, female parent and the problem of physiology injury of meeting.
In the time that people utilize the sterile-low temperature of high temperature leading (or promotion) leading (or promotion) can educate the sterile lines seed making of type, bring the risk in hybrid seed production of fertility restorer to male sterile line for avoiding the appearance of microthermal climate, often the sensitive periods of induction male sterile line fertility conversion, is arranged in to the season that in 1 year, temperature is the highest (or date section), but the high temperature of this date section equally also may have influence on the fertility of paternal pollen.And then sensitive periods one crosses is flowering stage, and the florescence also just especially easily meets high temperature so.Florescence high temperature can allow male parent pollen sterility, lose germinating capacity, also allow maternal column cap vigor decline, thus impact pollination [2].This is also that the male sterile line that utilizes sterile-low temperature of high temperature leading (or promotion) leading (or promotion) can educate type carries out a lower major reason of two hybrid seed yields while being the production of hybrid seeds.
In the time that the sterile-high temperature of people's application of cold temperature leading (or promotion) leading (or promotion) can be educated type sterile lines seed making, often male sterile line fertility is arranged in to early summer or season in late fall to the sensitive periods of light temperature reaction, low temperature before early summer occurs more in advance more frequently with what sow, serious low temperature removes paddy rice at seeding stage, equally also the tillering stage to paddy rice, fringe idiophase are until the adverse effect of having grown of the sensitive periods of fertility to thermotonus causes even maldevelopment and other physiology to damage to plants caused by sudden drop in temperature; Same, the low temperature after late fall also will occur more frequently with the pusher in season, low temperature also can bring adverse effect to paddy rice in the pollination at florescence, the substantial and maturation of the seed of pustulation period.
Generally speaking, while adopting existing two to be seeding technique, often (even high frequency time) can meet the problem of the adverse effect that too high or too low temperature brings, the adverse effect no matter these problems cause is to be greatly little, because existing two being that outdoor seeding technique fails the factor such as illumination and temperature effectively to manipulate, these problems that meet are always not enough to effective solution.
Summary of the invention
3. summary of the invention
The invention belongs to paddy rice seeding technique field, definite says, is pure keeping, the high-yield seeds production technique of two-line hybrid rice.During for the background technology production of hybrid seeds, effectively control and regulate the environmental factors such as the temperature of certain (several) individual important period production of hybrid seeds work to be brought to the problem of above-mentioned serious adverse effect because failing, the present invention proposes: " in the intermediary and later stages of male sterile line; application greenhouse, booth or shed technology are carried out the production of hybrid seeds ", this (content in these quotation marks) is exactly the technical characterictic that the present invention is different from prior art.Its concrete grammar and step are: " be in seed farm paddy rice two, certain of later stage or certain several growing stage meet maybe may meet too high or too low temperature and unfavorable illumination time, for avoiding, prevent and slow down unfavorable temperature in this period, the environmental factors such as illumination are to the father in the production of hybrid seeds, female parent brings adverse effect, or in order to give father, female parent is created more adapt circumstance condition, now, first use greenhouse, booth or shed cover (also can in early days rice seedling be planted in greenhouse or booth) to seed farm, repave with other relevant temperature adjustments, the auxiliary equipment of light modulation or method, make greenhouse, temperature in booth or shed, optical condition can meet production of hybrid seeds operation father, maternal requirement.”
First with regard to the unsolved problem of background technology, illustrate corresponding solution of the present invention below:
Example 1 anomalous weather causes fertility restorer way to solve the problem.
In the time of the production of hybrid seeds of application optical-and thermal-sensitive male nuclear sterile rice, if within the sensitive periods of induction optical-and thermal-sensitive male nuclear sterile rice fertility conversion (certain stage of 5-20 days before Rice Flowering) meet and may cause it to transfer the unfavorable illumination that can educate to, when temperature condition, first use greenhouse, booth or shed cover (also can in early days rice seedling be planted in greenhouse or booth) to seed farm, auxiliary equipment or the method with other the relevant temperature adjustment light modulations that repave, make greenhouse, temperature in booth or shed, optical condition all the time in can induce optical-and thermal-sensitive male nuclear sterile rice keep sterile and be suitable for the scope of growing of paddy rice within.Just it be can effectively solve thus in the production of hybrid seeds of optical-and thermal-sensitive male nuclear sterile rice and unfavorable temperature, the impact of illumination factor within sensitive periods of light temperature induction fertility conversion, are subject to and the problem that causes fertility restorer.
For clearer explanation the present invention one or two is technical characterictic in production of hybrid seeds key issue solving this, will give an example more below respectively and can educate sterile-high temperature of type leading with low temperature (or promotion) leading (or promotion) with regard to sterile-low temperature of high temperature leading (or promotion) leading (or promotion) and can educate type male sterile line and how to apply the elaboration that the present invention's work is more detailed.
1) long day leading sterile, high temperature promotes leadingly can educate for sterile-short day, low temperature promotes to educate type male sterile line and high temperature promotes that sterile-low temperature is leading for leading sterile, long day and can educate, within short day, promote to educate type male sterile line.
Such male sterile line is within the sensitive periods (being generally the 5-15 days before heading) of its fertility conversion of induction, if suddenly meet the low temperature that typhoon or other meteorologic factors are brought, if to be converted to the critical temperature value that can educate also low from sterile than its fertility of the induction of this male sterile line for this temperature value, so, we can in advance or find immediately greenhouse or the booth of planting male sterile line to be closed to window after microthermal climate, close the door, allow greenhouse or booth cover tight to seed farm, re-use temperature raising device or method heats, the critical temperature value that temperature in indoor or canopy is changed higher than male sterile line fertility, generally indoor (or in canopy) temperature requires more than 27 DEG C, but should not exceed 35 DEG C.If male sterile line is not planted in greenhouse or booth in advance, and in the time that the sensitive periods of fertility conversion meets abnormal low temperature, we can cover seed farm with dismountable (or portable) greenhouse (or booth), simple plastic greenhouse or shed, and then heat, add lustre to heating add lustre to equipment or method.For long day leading sterile, high temperature promote leadingly can to educate for sterile-short day, low temperature promotes to educate the dusk of type male sterile line within fertility conversion sensitive periods or while meeting rare total solar eclipse morning, need to add lustre to the equipment that adds lustre in greenhouse or booth during total solar eclipse.Treat bad weather or sensitive periods one mistake, we again removable chamber, scaffold dismantling or take off the ways such as film and carry out outdoor Routine Management.
2) sterile-high temperature of low temperature leading (or promotion) leading (or promotion) can be educated type male sterile line.
This class male sterile line in the sensitive periods (being generally the 5-20 days before heading) of its fertility of induction conversion if in suddenly meet the temperature higher than the critical temperature value of this male sterile line, so, we can in advance or find immediately greenhouse or the booth of planting this male sterile line to be closed window, closed the door after hot weather, allow greenhouse or booth cover tight to seed farm, re-use cooling system or method cooling, the critical temperature value that temperature in indoor or canopy is changed lower than male sterile line fertility, but should not be lower than 21 DEG C.If male sterile line is not planted in greenhouse or booth in advance, and in the time that the sensitive periods of fertility conversion meets abnormal high temperature, we can cover seed farm with dismountable (or portable) greenhouse (or booth), simple plastic greenhouse or shed, and then lower the temperature and reduce illumination by cooling system, shading device or method.Treat bad weather or sensitive periods one mistake, we again removable chamber, scaffold dismantling or take off the ways such as film and carry out outdoor Routine Management.
Example 2 anomalous weathers cause Rice Physiological injury way to solve the problem.
Background technology one joint is mentioned herein, and paddy rice two is the production of hybrid seeds, is especially easily subject to the harm of anomalous weather.For this class problem, we can be by temperature, illumination and the humidity that should be used for seed farm of greenhouse, booth or shed technology, finally make the environmental condition of seed farm can meet production of hybrid seeds requirement, avoid, prevent or slow down adverse weather factor to be the adverse effect of the production of hybrid seeds to two thereby reach.For example: 1), the sterile-low temperature of high temperature leading (or promotion) leading (or promotion) can be educated type sterile lines seed making.Such sterile lines seed making often easily meets more than 35 DEG C high temperature flowering stage, and more than 35 DEG C high temperature of florescence easily causes maternal column cap vigor to decline, and the fertility of paternal pollen reduces, the lost of life.If now meet high temperature, according to technical characterictic of the present invention, be that we can first cover seed farm at the florescence, then, use again cooling system as air-conditioning temperature-reducing, also can reduce indoor (or in film) temperature by rear shading before midday, spraying cold water, the method such as film, draining of taking off before the dusk, reach with this that to avoid, prevent, alleviate florescence hot weather be the negative effect that the production of hybrid seeds brings to two, make the factor such as illumination, temperature of indoor (or in canopy) be suitable for rice growth and other physiological requirements.2) sterile-high temperature of low temperature leading (or promotion) leading (or promotion) can be educated type sterile lines seed making.If such male sterile line fertility-sensitive period is arranged in early summer, before sensitive periods, each growing stage is easy to meet low temperature, and now low temperature easily causes rice growth obstacle and physiology damages to plants caused by sudden drop in temperature, and can solve problems just utilize greenhouse, booth or the insulation of shed technology to heat.And if fertility-sensitive period is arranged in season in late fall, now, we also can apply greenhouse, booth or shed technology and heat until the maturing stage is also easy to suffer chilling injury the florescence after sensitive periods, Grain Filling phase, reached and prevented the object that damages to plants caused by sudden drop in temperature.
Generally speaking, we not only can be in the fertility of paddy rice early stage (seeding stage) application greenhouse, booth or shed technology prevent the harm of low temperature to rice seedling, be the paddy rice production of hybrid seeds for two, we can also be paddy rice father, maternal to temperature, the growth key elements such as light have other periods of special requirement, application greenhouse, booth or shed and corresponding establishment thereof are incubated, heat, cooling, add lustre to, shading, moisturizing, damping, rainproof, windproof, anti-non-paternal pollen, insect protected and diseases prevention etc., avoid thereby reach, prevent or alleviate the object of the adverse effect of adverse environmental factors to Parent.
Be production of hybrid seeds paddy rice middle and later periods application greenhouse, booth or shed technology two, above-mentionedly can avoid, prevent or slow down male sterile line and above-mentionedly can avoid, prevent or slow down the effect that abnormal weather factor causes father, maternal physiology injury etc. because meeting abnormal weather factor and cause effect repeatedly of fertility and having except having, also there is following beneficial effect:
1, the restriction of the light temperature critical value of the type to male sterile line and fertility conversion is more not harsh.This makes the higher or on the low side male sterile line that can not be used for the production of hybrid seeds of original many temperature threshold value because of the conversion of induction fertility and the two poles of the earth male sterile line can be used for the production of hybrid seeds, thereby in breeding, has increased seed selection to the probability of hybrid combination with Super-high-yielding or other merits.
2, can be more flexible to the male sterile line induction fertility conversion season of sensitive periods or the arrangement of date section.We can also take into account or lay particular emphasis on and avoid or alleviate heat evil or the extreme low temperature of thermal extremes to paddy rice arranging maternal fertility to change the period of sensitive periods to the angle damaging to plants caused by sudden drop in temperature of paddy rice, just can reduce thus the underproduction and increase hybrid seed yield.The flexible arrangement in production of hybrid seeds season also helps the front succession crop reasonable arrangement in sowing time.
3, more wide in range to the selection of production of hybrid seeds region.
4, utilize idle, half idle vegetables in improper season greenhouse (or booth) or other classes are idle, to carry out two be the production of hybrid seeds in half idle greenhouse (or booth), not only can save greenhouse, booth cost, improve greenhouse, booth service efficiency, and through rice field-upland field rotation, can also improve soil property, alleviate the soil salinization, reduce the disease pest and weed of Dry crop and paddy rice.
Finally, we wait in expectation people can from transformation " gene " start with, select desirable male sterile line; Can start with from transformation " environment " again, application greenhouse, booth or the production of hybrid seeds of shed technology.The two perfect adaptation, is bound to allow this " gene " and " environment " do mutually male nuclear sterile type paddy rice and bears rich fruit in vast, richly endowed, the spectacular large ground of motherland.
Bibliography:
[1] the capable Shen Yuezheng of Xue Guang " the light temperature binding mode of the photosensitive sterile rice fertility conversion of examination view " " Acta Agronomica Sinica " the 21st volume Chinese Academy of Agricultural Sciences's crop breeding in the 2nd March nineteen ninety-five phase cultivation research institute.
[2] Zhang Bin 1rui Wenyi 1zheng Jianchu 2deng " rice anthesis powder vigor and the ripening rate response characteristic to high temperature " " Acta Agronomica Sinica " 2007,33 (7) 1177-1181 12 Jiangsu Academy of Agricultural Sciences of Agricultural University Of Nanjing.

Claims (1)

1. the pure keeping of two-line hybrid rice, a seed making method, its technical characterictic is: be the seed farm paddy rice intermediary and later stages two, used greenhouse, booth or shed technology to carry out the production of hybrid seeds.
CN201310375565.3A 2013-08-27 2013-08-27 High-yield and purity-maintaining seed production technique of two-line hybrid rice Pending CN103843555A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105638347A (en) * 2016-01-20 2016-06-08 安徽理想种业有限公司 Rice two-line sterility line propagation method
CN107372100A (en) * 2017-08-23 2017-11-24 四川省农业科学院水稻高粱研究所 A kind of method of Sorghum Hybrid breeding
CN109258170A (en) * 2018-09-20 2019-01-25 湖北省农业科学院粮食作物研究所 A kind of new method of breeding rice low-temperature-sensitive two-line sterile line

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Publication number Priority date Publication date Assignee Title
CN1055098A (en) * 1990-03-29 1991-10-09 福建农学院 The improved route of two-line hybrid rice
CN1561692A (en) * 2004-04-20 2005-01-12 河北省农垦科学研究所 Method for preparing two-line hybrid rice seeds by utilizing ecological male sterility
CN101622958A (en) * 2008-07-10 2010-01-13 吴和生 Method for breeding fragrant glutinous rice paddy seed
CN102144533A (en) * 2010-12-27 2011-08-10 湖北省农业科学院粮食作物研究所 Method for cultivating two-line sterile line rice with long fertility conversion transition period

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1055098A (en) * 1990-03-29 1991-10-09 福建农学院 The improved route of two-line hybrid rice
CN1561692A (en) * 2004-04-20 2005-01-12 河北省农垦科学研究所 Method for preparing two-line hybrid rice seeds by utilizing ecological male sterility
CN101622958A (en) * 2008-07-10 2010-01-13 吴和生 Method for breeding fragrant glutinous rice paddy seed
CN102144533A (en) * 2010-12-27 2011-08-10 湖北省农业科学院粮食作物研究所 Method for cultivating two-line sterile line rice with long fertility conversion transition period

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105638347A (en) * 2016-01-20 2016-06-08 安徽理想种业有限公司 Rice two-line sterility line propagation method
CN107372100A (en) * 2017-08-23 2017-11-24 四川省农业科学院水稻高粱研究所 A kind of method of Sorghum Hybrid breeding
CN109258170A (en) * 2018-09-20 2019-01-25 湖北省农业科学院粮食作物研究所 A kind of new method of breeding rice low-temperature-sensitive two-line sterile line

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Application publication date: 20140611