CN107372100A - A kind of method of Sorghum Hybrid breeding - Google Patents

A kind of method of Sorghum Hybrid breeding Download PDF

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Publication number
CN107372100A
CN107372100A CN201710760447.2A CN201710760447A CN107372100A CN 107372100 A CN107372100 A CN 107372100A CN 201710760447 A CN201710760447 A CN 201710760447A CN 107372100 A CN107372100 A CN 107372100A
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China
Prior art keywords
sorghum
parent
male
seed
female
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CN107372100B (en
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龙文靖
倪先林
刘天朋
赵甘霖
汪小楷
丁国祥
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RICE AND SORGHUM INSTITUTE SICHUAN ACADEMY OF AGRICULTURAL SCIENCES
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RICE AND SORGHUM INSTITUTE SICHUAN ACADEMY OF AGRICULTURAL SCIENCES
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a kind of method of Sorghum Hybrid breeding, comprise the following steps：October and mid-October sow sorghum material respectively, make sorghum booting florescence Daily minimum temperature respectively in 15 20 DEG C and 10 15 DEG C；It is female parent to select equal male sterility under sowing condition twice, the fertile material of female, and male fertile material is male parent under sowing condition twice；Advantageous combination is carried out to the female parent material and male parent material screened, first familiar generation seed is obtained, F1 seeds is sowed, remove F1 generation false hybrid, and bagging is selfed to obtain F2 for seed, and strain bagging selfing consistent with objective trait is selected from offspring, produces preferable self-mating system.The present invention can utilize restorer, maintainer, cenospecies, conventional kind male sterility and conventional kind, restorer, maintainer, cenospecies hybridization under the conditions of special climate, reduce artificial emasculation workload, and ensure hybrid seed quantity, it is expected to select the Parents In Sorghum material of high yield and high quality better resistance.

Description

A kind of method of Sorghum Hybrid breeding
Technical field
The present invention relates to genetic breeding field, and in particular to a kind of method of Sorghum Hybrid breeding.
Background technology
Sorghum [Sorghum bicolor (L.) Moench] is one of important cereal crop of China with drought-enduring, resistance to The multiple resistances such as flood, impoverishment tolerant, Salt And Alkali Tolerance, Plain, massif, salt-soda soil can plant, and have high biological yield and economy Yield.Sorghum is mainly to carry out heterosis utilization with " three systems " system, and the selection of Parents system is apolegamy superior hybrid crosses The key of combination.If parent owner is by crossbreeding seed selection at present, in its Breeding Process, artificial emasculation workload is big, behaviour Make cumbersome, efficiency is low, and hybrid seed amount is few.
The content of the invention
To solve the above problems, the invention provides a kind of method of Sorghum Hybrid breeding, can by restorer, maintainer, Conventional kind, cenospecies are converted into sterile and a large amount of restorers, maintainer or conventional sorghum material and hybridized, it is expected to it is excellent to select high yield The Parents In Sorghum material of matter better resistance.
To achieve the above object, the technical scheme taken of the present invention is：
A kind of method of Sorghum Hybrid breeding, comprises the following steps：
S1, October and mid-October sow sorghum material in Yuanjiang County of Yunnan respectively, are selected according to different materials breeding time The specific sowing time so that the sorghum florescence minimum temperature sowed twice is respectively in 15-20 DEG C and 10-15 DEG C；It will broadcast twice Male sterility, the fertile material of female be as maternal under the conditions of kind, will male fertile material is made under sowing condition twice For male parent；
S2, advantageous combination is carried out to the female parent material and male parent material screened
According to breeding objective, the Parent material with objective trait is selected, is sowed Second Year October in Yuanjiang County of Yunnan, And make it that the Parent florescence is consistent according to adjustment date of seeding Parent breeding time；Then bagging processing is carried out to female parent, in Post flowering Male parent material pollen is taken to be pollinated, so as to obtain first familiar generation seed；
S3, filial generation is cultivated and selected
The F1 generation seed of gained is sowed, removes F1 generation false hybrid, and bagging is selfed to obtain F2 for seed, from F2 for seed Offspring in the selfing of selection target character consistent strain bagging, produce preferable self-mating system.
The breeding method of the present invention can be using restorer, maintainer, cenospecies, conventional kind male under the conditions of special climate not Educate and hybridize with conventional kind, restorer, maintainer, cenospecies, reduce artificial emasculation workload, and ensure hybrid seed quantity.
Embodiment
In order that objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further Describe in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair It is bright.
The applicant has found that sorghum, which runs into relative low temperature in florescence, can then cause sorghum male in long-term sorghum is studied Infertility, and obvious genotypic difference between different self-mating systems be present.Can be in special ecological point by fertility using genotypic difference The higher self-mating system of inversion temperature does female parent, and the relatively low self-mating system of Fertility Transfer Temperature does male parent and carries out advantage hybridization, so as to Select and meet breeding objective self-mating system, specifically, comprising the following steps：
S1, existing sorghum material screened
October and mid-October sow sorghum material in Yuanjiang County of Yunnan respectively, are selected according to different materials breeding time specific Sowing time so that the sorghum florescence minimum temperature sowed twice is respectively in 15-20 DEG C and 10-15 DEG C；Bar will be sowed twice Equal male sterility under part, the fertile material of female as maternal, will twice under sowing condition male fertile material as father This；
S2, advantageous combination is carried out to the female parent material and male parent material screened
According to breeding objective, the Parent material with objective trait is selected, is sowed Second Year October in Yuanjiang County of Yunnan, And make it that the Parent florescence is consistent according to adjustment date of seeding Parent breeding time；Then bagging processing is carried out to female parent, in Post flowering Male parent material pollen is taken to be pollinated, so as to obtain first familiar generation seed；
S3, filial generation is cultivated and selected
The F1 generation seed of gained is sowed, removes F1 generation false hybrid, and bagging is selfed to obtain F2 for seed, from F2 for seed Offspring in the selfing of selection target character consistent strain bagging, produce preferable self-mating system.
Embodiment 1
S1, existing sorghum material screened
October and mid-October sow sorghum material in Yuanjiang County of Yunnan respectively, are selected according to different materials breeding time specific Sowing time, make sorghum florescence Daily minimum temperature respectively in 15-20 DEG C and 10-15 DEG C；Select male under sowing condition twice Property infertility material for female parent, normal material is male parent under sowing condition twice；
S2, advantageous combination is carried out to the female parent material and male parent material screened
According to breeding objective, male parent material 405B and the preferable female parent material of yield traits with certain short source are selected 45B, continue to sow in Yuanjiang County of Yunnan October in Second Year, and the Parent florescence one is caused according to adjustment date of seeding Parent breeding time Cause.Carry out bagging to 45B takes 405B material pollen to pollinate in Post flowering, so as to obtain first familiar generation seed.
S3, filial generation is cultivated and selected
F1 seeds are sowed, remove F1 generation false hybrid, and bagging is selfed to obtain F2 for seed, the constantly selection strain from offspring Blade profile is good, the higher strain bagging selfing of yield, finally gives preferable self-mating system 1509B.
Embodiment 2
S1, existing sorghum material screened
October and mid-October sow sorghum material in Yuanjiang County of Yunnan respectively, are selected according to different materials breeding time specific Sowing time, make sorghum florescence Daily minimum temperature respectively in 15-20 DEG C and 10-15 DEG C；Select male under sowing condition twice Property infertility material for female parent, normal material is male parent under sowing condition twice；
S2, advantageous combination is carried out to the female parent material and male parent material screened
According to breeding objective, male parent material 272R with certain short source and breeding time female parent material earlier are selected 1609A × 186R, sowed November then in Hainan Lingshui base, and Parent is caused according to adjustment date of seeding Parent breeding time Florescence is consistent.Carry out bagging to cenospecies 1609A × 186R takes 272R material pollen to pollinate in Post flowering, so as to obtain 09A × 186R × 272R triple hybrids；
S3, filial generation is cultivated and selected
To constantly selection strain bagging selfing consistent with objective trait in triple hybrid offspring, preferable selfing is finally given System.
Described above is only presently preferred embodiments of the present invention, it is noted that for those skilled in the art For, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications should also regard For protection scope of the present invention.

Claims (1)

  1. A kind of 1. method of Sorghum Hybrid breeding, it is characterised in that comprise the following steps：
    S1, October and mid-October sow sorghum material in Yuanjiang County of Yunnan respectively so that the sorghum florescence sowed twice is most Low temperature is respectively in 15-20 DEG C and 10-15 DEG C；Equal male sterility under sowing condition twice, the fertile material of female are incited somebody to action as female This, will twice under sowing condition male fertile material as male parent；
    S2, advantageous combination is carried out to the female parent material and male parent material screened
    According to breeding objective, the Parent material with objective trait is selected, is sowed Second Year October in Yuanjiang County of Yunnan, and root Make it that the Parent florescence is consistent according to adjustment date of seeding Parent breeding time；Then bagging processing is carried out to female parent, father is taken in Post flowering This material pollen is pollinated, so as to obtain first familiar generation seed；
    S3, filial generation is cultivated and selected
    The F1 generation seed of gained is sowed, removes F1 generation false hybrid, and bagging is selfed to obtain F2 for seed, from F2 for seed after The consistent strain bagging selfing of selection target character, produces preferable self-mating system in generation.
CN201710760447.2A 2017-08-23 2017-08-23 A kind of method of Sorghum Hybrid breeding Active CN107372100B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108739349A (en) * 2018-03-13 2018-11-06 四川省农业科学院水稻高粱研究所 A kind of selection of sorghum restorer line
CN112753571A (en) * 2021-03-15 2021-05-07 四川省农业科学院水稻高粱研究所 Breeding method of sorghum cytoplasmic male sterile line with high tannin content

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1276149A (en) * 1999-06-03 2000-12-13 湖南省农业科学院高粱研究开发中心 Two-line hybridization method for producing sorghum seeds
CN1283385A (en) * 2000-09-15 2001-02-14 朱永峰 Method fro selective cultivation, reproduction and seed preparation of hydridized sorghum with new combination
CN101288380A (en) * 2008-03-19 2008-10-22 广东省农科集团良种苗木中心 Good quality, high yield, disease-resistant and wide adaptation ultra-sweet corn breeding method
CN102835301A (en) * 2012-02-01 2012-12-26 吉林省农业科学院 Breeding, seed-producing and cultivating method for sorghum coeno-species Jiza 130
CN103843555A (en) * 2013-08-27 2014-06-11 肖卓丽 High-yield and purity-maintaining seed production technique of two-line hybrid rice

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1276149A (en) * 1999-06-03 2000-12-13 湖南省农业科学院高粱研究开发中心 Two-line hybridization method for producing sorghum seeds
CN1283385A (en) * 2000-09-15 2001-02-14 朱永峰 Method fro selective cultivation, reproduction and seed preparation of hydridized sorghum with new combination
CN101288380A (en) * 2008-03-19 2008-10-22 广东省农科集团良种苗木中心 Good quality, high yield, disease-resistant and wide adaptation ultra-sweet corn breeding method
CN102835301A (en) * 2012-02-01 2012-12-26 吉林省农业科学院 Breeding, seed-producing and cultivating method for sorghum coeno-species Jiza 130
CN103843555A (en) * 2013-08-27 2014-06-11 肖卓丽 High-yield and purity-maintaining seed production technique of two-line hybrid rice

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
何觉民 等: "《生态遗传雄性不育理论与两系杂交作物》", 31 May 1997, 湖南科学技术出版社 *
倪先林 等: "杂交糯高粱品种川糯粱1号高产制种技术", 《种子》 *
张天真: "《作物育种学总论》", 31 March 2014, 中国农业出版社 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108739349A (en) * 2018-03-13 2018-11-06 四川省农业科学院水稻高粱研究所 A kind of selection of sorghum restorer line
CN112753571A (en) * 2021-03-15 2021-05-07 四川省农业科学院水稻高粱研究所 Breeding method of sorghum cytoplasmic male sterile line with high tannin content

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