CN107410015A - A kind of selection of leafy mustard cytoplasmic male sterile line - Google Patents
A kind of selection of leafy mustard cytoplasmic male sterile line Download PDFInfo
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- CN107410015A CN107410015A CN201710842019.4A CN201710842019A CN107410015A CN 107410015 A CN107410015 A CN 107410015A CN 201710842019 A CN201710842019 A CN 201710842019A CN 107410015 A CN107410015 A CN 107410015A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention provides a kind of selection of leafy mustard cytoplasmic male sterile line, including：Using the natural 102A of mustard type rape cytoplasmic male sterility 6 as female parent, maintainer leafy mustard conventional variety is that male parent is hybridized, and obtains the leafy mustard cytoplasmic male sterile line.The leafy mustard cytoplasmic male sterile line obtained using the selection of the present invention has the characteristics of sterility is stable, thorough and not affected by environment, overcomes the defects of existing brassicaceous vegetable cytoplasmic male sterile line sterility is unstable.
Description
Technical field
The present invention relates to brassicaceous vegetable breeding technical field, more particularly, to a kind of leafy mustard cytoplasm male
Property sterile line breeding method.
Background technology
Brassicaceous vegetable hybrid vigour is obvious, the main path of heterosis utilization for self-incompatibility and male not
Fertility, wherein cytoplasmic male sterility are preferable pollination control systems.By using the male sterility factor of separate sources, choosing
Individual cabbage heart cytoplasm male sterility line stable to environment and ontogeny in growth course is educated, and is bred and utilized,
Make the excellent first generation of hybrid of Comprehensive Traits.The brassicaceous vegetable first generation of hybrid is widely carried out using work, particularly
In Asian countries, such as China, Japan and South Korea, hybrid is carried out using the method for self incompatible line or self compatible line at present
Generation production is in the majority, but this method there is incompatibility be difficult to keep or be selfed more generations easily occur the decline of parents' viability with
Easily there is the problems such as pseudostationary.Using the cytoplasmic male sterile line production of hybrid seeds, the filial generation of high quality can be obtained.It is raw at present
The brassicaceous vegetable cytoplasmic male sterility type utilized in production is mainly rape pol cytoplasmic male sterility (pol cms) (pol
) and Ogura cytoplasmic male sterility (ogu CMS), CMS but there is sterility in rape pol cytoplasmic male sterility (pol cms) (pol CMS)
It is unstable, influenceed the shortcomings that pollen amount is few, seed purity is low easily occur by bad temperature；Low temperature yellow and honey be present in ogu CMS
The shortcomings of gland is degenerated, has been improved at present.Therefore new cytoplasmic male sterility type is excavated and formulated, solves cytoplasm
Single-issue, existing germ plasm resource is widened, is had great importance.
Pol CMS are that Hua Zhong Agriculture University Fu Ting finds for 1972, sharp extensively in rape and brassicaceous vegetable
With Peng Qian etc. is maternal for sterile source using Brassica napus male sterile line Hunan oil A male sterile plant, with 4 9-No. 19 dishes
The heart is male parent, has been bred as cabbage heart Alloplasmic male sterile system 002-8-20A and its maintainer.Ogu is found from Ogura1968
Since CMS, domestic and foreign scholars have carried out substantial amounts of backcross transformation to the sterile material, transformation to rape at present, wild cabbage, white
In the brassicaceous vegetables such as dish, broccoli.After Fang Zhiyuan introduces the new improvement of 6 parts of CMS625, CMS629 etc. in 1998 from the U.S.
Ogu CMS materials, and carry out transformation with more than 30 part wild cabbage self-mating systems and more than 20 part broccoli self-mating systems, after CMS625, CMS629
The advantages that now not yellow of low temperature lower blade, sterile flower open is represented, there is preferable application prospect.
Leaf mustard is originating from China, and because leaf mustard floral organ is small, therefore CMS is the main path of leaf mustard heterosis utilization.
The leaf mustard cytoplasmic male sterility type reported both at home and abroad at present mainly has leaf mustard hau CMS (ten thousand positive outstanding persons etc.), Moricandia
Arvevsis CMS (Prakash et al), leaf mustard oxa CMS (history China clear etc.) and hot pickled mustard tube CMS (Chen Zhujun etc.).Leaf mustard hau
CMS is a kind of new cytoplasmic male sterility type that Hua Zhong Agriculture University 1999 finds, by leaf mustard hau CMS not
Fertility evaluation, the Molecular Identification of mitochondria sterile gene, sterile gene clone, transgenosis functional verification and mitochondrial genomes are surveyed
Sequence, specify that leaf mustard hau CMS is a kind of crop in cruciferae cytoplasmic sterility new type.Chen Zhujun etc. (1993) with nineteen eighty-three from
(leaf mustard × Chinese cabbage) interspecific hybrid sterile source that the Chinese Academy of Agricultural Sciences vegetables are introduced, it is male that backcross transformation obtains preserved sichuan pickle cytoplasm
Property sterile line.On this basis, the sterile gene orf220 of the sterile line is obtained using homologous sequence method.
The content of the invention
In order to overcome, the existing leafy mustard cytoplasmic male sterile line procedure of breeding is complicated, breeding efficiency is not high and sterile
The unstable deficiency of property, the invention provides the selection of leafy mustard cytoplasmic male sterile line, this method includes：
It is by female parent, maintainer leafy mustard conventional variety of natural mustard type rape cytoplasmic male sterility 6-102A
Male parent is hybridized, and obtains above-mentioned leafy mustard cytoplasmic male sterile line.
The present invention is female parent from natural mustard type rape cytoplasmic male sterile line 6-102A, with maintainer leafy mustard
Conventional variety hybridizes, and obtains the leafy mustard cytoplasmic male sterile line and maintainer of inheritance stability, it is thin to breed the leafy mustard
Cytoplasm male sterility line and combo obtain being available for the cenospecies of Commercial cultivation.
Wherein, using above-mentioned selection, the procedure of breeding is simplified, specify that breeding objective, improve breeding efficiency, and
The sterility of obtained leafy mustard cytoplasmic male sterile line is stable and thorough, not affected by environment, in Gansu, China Lanzhou
5 year 10 generation continuous with Hubei Wuhan, which plant and carry out Fertility identification, to be shown, its fertility shows 0 grade, sterile plant rate and sterile degree
It is 100%, and phenomena such as no any dead flower bud and seedling stage yellow.
Wherein, the conventional variety that maintainer leafy mustard conventional variety can be selected in this area, preferably leafy mustard
Conventional variety orchid W102.
Wherein, in a preferred embodiment, preferably it is from natural juncea cytoplasmic male sterile line 6-102A
Female parent, hybridize with maintainer leafy mustard conventional variety, by continuous backcross, so as to obtain more stable leafy mustard cytoplasm
Male sterile line and maintainer.Wherein, continuous backcross at least 4 generations, further preferably at least 8 generations.Recurrent parent is preferably to keep
It is leafy mustard conventional variety, such as leafy mustard conventional variety orchid W102.
In a preferred embodiment of the invention, in order to obtain more stable leafy mustard cytoplasmic male sterile line,
It is preferred that using using natural juncea cytoplasmic male sterility 6-102A as female parent, leafy mustard conventional variety orchid W102 is samsara
Parent, by continuous backcross seed selection, to obtain leafy mustard cytoplasmic male sterile line.
Wherein, preferred steps include：
(1) described natural juncea male sterile plant 6-102A is used to make female parent, with maintainer leafy mustard conventional variety
Make male parent, hybridization obtains Hybrids F1；
(2) Fertility identification is carried out to Hybrids F1, the F1 plant obtained with step (1) are female parent, with above-mentioned maintainer great Ye
Leaf mustard conventional variety makees male parent, and hybridization obtains BC1F1 CMS.
(3) Fertility identification is carried out to BC1F1, the BC1F1 CMS obtained with step (2) make female parent, big with above-mentioned maintainer
Leaf mustard conventional variety makees male parent, obtains BC2F1 cytoplasmic male sterile lines；
(4) Fertility identification, the BC2F1 cytoplasmatic males obtained with step (3) are carried out to BC2F1 cytoplasmic male sterile lines
Sterile line is female parent, makees male parent with above-mentioned maintainer leafy mustard conventional variety, hybridization obtains BC3F1 cytoplasmic male sterilities
System；
(5) Fertility identification is carried out to BC3F1 cytoplasmic male sterile lines；The BC3F1 cytoplasmatic males obtained with step (4)
Sterile line makees female parent, makees the generation of recurrent parent continuous backcross 5 with above-mentioned maintainer leafy mustard conventional variety, it is thin to obtain leafy mustard
Cytoplasm male sterility line orchid W101.
In the above-mentioned methods, male parent and maternal row ratio both preferably 2:4.
Wherein, selection of the invention is preferably carried out under the conditions of autumn sowing or summer sowing isolation.
After obtaining the leafy mustard cytoplasmic male sterile line of above-mentioned inheritance stability, the leafy mustard cytoplasmatic male is bred
Sterile line and combo obtain being available for the cenospecies of Commercial cultivation.
Wherein, leafy mustard cytoplasmic male sterile line propagation steps are：
In seed autumn sowing (such as annual September) or the summer sowing of Gansu, China Lanzhou (such as annual 5 of Hubei China Wuhan
Month) isolation under the conditions of, by male parent：Maternal row ratio is 2：4 planting proportion breeding leafy mustard cytoplasmic male sterile line, other
Breeding of the cultivation condition with common sterile line.
The selection of the leafy mustard cytoplasmic male sterile line of stable fertility proposed by the present invention, it is leafy mustard vegetable
Dish heterosis utilization provides new germ plasm resource.Utilize the infertility of the leafy mustard cytoplasmic male sterile line of seed selection of the present invention
The advantages that property is stable, thorough and not affected by environment, cultivates new leafy mustard cytoplasmic male sterility material and vegetables is miscellaneous
Hand over kind.
Brief description of the drawings
Fig. 1 is according to Technology Roadmap in the embodiment of the present invention 1；
Fig. 2 is to be schemed according to the floral organ of new mustard type rape cytoplasmic male sterile line 6-102A in the embodiment of the present invention 1
With the floral organ and individual plant figure of leafy mustard cytoplasmic male sterile line (blue W101) and maintainer (blue W102)；
In figure：A is new mustard type rape cytoplasmic male sterile line 6-102A floral organ figure；B, C is that leafy mustard is thin
The floral organ of cytoplasm male sterility line and maintainer is schemed；D, E is the individual plant of leafy mustard cytoplasmic male sterile line and maintainer
Figure.
Embodiment
With reference to embodiment, the embodiment of the present invention is described in further detail.Following examples are used for
Illustrate the present invention, but be not limited to the scope of the present invention.
Unless otherwise specified, the routine techniques hand that technological means used in embodiment is well known to those skilled in the art
Section.Unless otherwise specified, reagent used in embodiment is commercially available.
The seed selection of the leafy mustard cytoplasmic male sterile line of embodiment 1
The Technology Roadmap of the present embodiment selection is as shown in Figure 1.
(1) described natural mustard type rape male sterile line 6-102A is used to make female parent, it is blue with great Ye spring vegetables conventional variety
W102 (maintainer, commercially available) makees male parent, and hybridization obtains Hybrids F1；
(2) Fertility identification is carried out to Hybrids F1, the F1 plant obtained with step (1) are female parent, with great Ye spring vegetable routine product
The blue W102 (maintainer) of kind makees male parent, and hybridization obtains BC1F1CMS；
(3) Fertility identification is carried out to BC1F1, the BC1F1 CMS obtained with step (2) make it is maternal, it is and normal with great Ye spring vegetables
Rule kind orchid W102 (maintainer) makees male parent, obtains BC2F1 cytoplasmic male sterile lines；
(4) Fertility identification is carried out to BC2F1 cytoplasmic male sterile lines, the BC2F1 CMS obtained with step (3) make female
This, makees male parent, hybridization obtains BC3F1 cytoplasmic male sterile lines with great Ye spring vegetables conventional variety (maintainer)；
(5) Fertility identification is carried out to BC3F1 cytoplasmic male sterile lines；The BC3F1 CMS obtained with step (4) make female
This, makees the generation of recurrent parent continuous backcross 5 with maintainer, obtains BC8 great Ye spring vegetable cytoplasmic male sterile line orchids W101.
The breeding of the great Ye spring vegetable cytoplasmic male sterile lines of embodiment 2
In seed autumn sowing (such as annual September) or the summer sowing of Gansu, China Lanzhou (such as annual 5 of Hubei China Wuhan
Month) isolation under the conditions of, by male parent：Maternal row ratio is 2：4 planting proportion breeding great Ye spring vegetable cytoplasmic male sterile lines, other
Breeding of the cultivation condition with common sterile line.
Wherein, as shown in Figure 2：A is that mustard type rape cytoplasmic male sterile line 6-102A with leafy mustard is same
The different mutation of leaf mustard kind, represent the female parent material that the selection of leafy mustard cytoplasmic male sterile line uses.B, C is big
The floral organ of leaf mustard cytoplasmic male sterile line and maintainer scheme, using mustard type rape cytoplasmic male sterile line 6-102A as
Female parent, with blue W102 (maintainer) for male parent, by hybridization and continuous backcross technology, obtain leafy mustard cytoplasmic male sterility
System, in Wuhan Post flowering in March, observes its sterility, the leafy mustard sterile line orchid W101 infertility plans bottom of acquisition, Anther Abortion.
There is no pollen, maintainer orchid W102 (maintainer) floral organ is normal, there is fertile pollen.D, E be leafy mustard cytoplasmatic male not
It is individual plant figure with maintainer to educate, and shows, by hybridization technique and the generation of continuous backcross 5, to obtain leafy mustard cytoplasmic male sterility
System and corresponding maintainer economical character are basically identical, meet the genetic characteristicses of backcross progeny.
Finally, method of the invention is only preferable embodiment, is not intended to limit the scope of the present invention.It is all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvements made etc., the protection of the present invention should be included in
Within the scope of.
Claims (8)
- A kind of 1. selection of leafy mustard cytoplasmic male sterile line, it is characterised in that including：Using natural mustard type rape cytoplasmic male sterility 6-102A as female parent, maintainer leafy mustard conventional variety is male parent Hybridized, obtain the leafy mustard cytoplasmic male sterile line.
- 2. selection according to claim 1, it is characterised in that the maintainer leafy mustard conventional variety is great Ye Leaf mustard conventional variety orchid W102.
- 3. selection according to claim 1 or 2, it is characterised in that the hybridization is continuous backcross at least 4 generations, excellent Select at least 8 generations.
- 4. selection according to claim 3, it is characterised in that the recurrent parent is that maintainer leafy mustard is conventional Kind.
- 5. selection according to claim 1, it is characterised in that with natural mustard type rape cytoplasmic male sterility 6-102A is female parent, and leafy mustard conventional variety orchid W102 is recurrent parent, by continuous backcross seed selection, obtains the cruciate flower Section's vegetable plant cells matter male sterile line.
- 6. selection according to claim 1 or 2, it is characterised in that including：(1) make female parent with the natural juncea male sterile plant 6-102A, male parent made with maintainer leafy mustard conventional variety, Hybridization obtains Hybrids F1；(2) Fertility identification is carried out to Hybrids F1, the F1 plant obtained with step (1) are female parent, with the maintainer leafy mustard Conventional variety makees male parent, and hybridization obtains BC1F1CMS；(3) Fertility identification is carried out to BC1F1, the BC1F1CMS obtained with step (2) makees female parent, with the maintainer leafy mustard Conventional variety makees male parent, obtains BC2F1 cytoplasmic male sterile lines；(4) Fertility identification, the BC2F1 cytoplasmic male sterilities obtained with step (3) are carried out to BC2F1 cytoplasmic male sterile lines It is for female parent, male parent is made with the maintainer leafy mustard conventional variety, hybridization obtains BC3F1 cytoplasmic male sterile lines；(5) Fertility identification is carried out to BC3F1 cytoplasmic male sterile lines；The BC3F1 cytoplasmic male sterilities obtained with step (4) System makees female parent, makees the generation of recurrent parent continuous backcross 5 with the maintainer leafy mustard conventional variety, it is thin to obtain the leafy mustard Cytoplasm male sterility line orchid W101.
- 7. according to the selection any one of claim 1-6, it is characterised in that the male parent and maternal row ratio are 2:4。
- 8. according to the selection any one of claim 1-7, it is characterised in that methods described autumn sowing or summer sowing every Carried out under the conditions of.
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| CN112725497A (en) * | 2021-01-12 | 2021-04-30 | 华中农业大学 | Molecular marker for identifying stem mustard oxa cytoplasmic male sterility restoring gene and application thereof |
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