CN105284592B - A kind of method of Ogura cytoplasmic male sterility system seed selection - Google Patents
A kind of method of Ogura cytoplasmic male sterility system seed selection Download PDFInfo
- Publication number
- CN105284592B CN105284592B CN201510662941.6A CN201510662941A CN105284592B CN 105284592 B CN105284592 B CN 105284592B CN 201510662941 A CN201510662941 A CN 201510662941A CN 105284592 B CN105284592 B CN 105284592B
- Authority
- CN
- China
- Prior art keywords
- maintainer
- sterile
- plant
- rate
- transformation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The method that the present invention provides a kind of seed selection of Ogura cytoplasmic male sterility system, including hybridization produce F1；Hybridization produces BC1F1；Hybridization produces BC2F1；Hybridization produces BC3F1；Sterile line breeding completes totally five steps；Had the following advantages that with seed selection Ogura cytoplasmic male sterility system of the present invention：(1) this method is saved manually during transformation using entomophilas such as wall honeybees, expands transformation scale, can effectively exclude unstable maintenance maintainer.(2) present invention is carried out plus generation using the mixed mode handed over of maintainer, can Selfing avoidance decay, while using insect pollination, reducing manually.
Description
Technical field
Field, more particularly to a kind of side of Ogura cytoplasmic male sterility system seed selection are cultivated the present invention relates to Heterosis In Radish
Method.
Background technology
Radish (Raphanus sativus L.) also known as radish, reed Fu, are that the Constantly allogamous of Cruciferae Rhaphanus is planted
Thing.Radish is originating from China, and cultivation history is long, distributed more widely, and germ plasm resource is enriched, with significant hybrid vigour.Using miscellaneous
The advantage of kind can increase substantially yield and improving quality, and male sterility is the important channel using hybrid vigour, pure with seed
Degree is high, save the advantage of manpower.It is premise and the basis using hybrid vigour to cultivate new sterile line.In the practices of breeding, turn
Educate is the original sterile line defect of improvement, the important process of the excellent sterile line of acquired character.Traditionally the transformation of radish sterile line needs people
Work carries out substantial amounts of hybridization, backcrossing screening operation.Quickly, it is urgent need solution in radish crossbreeding work accurately to set up sterile line
Technical problem.
The male sterile genetic background of radish is complicated, and different germplasm have different male sterility genes, in transformation sterile line
During, the holding capacity that difference plants confrontation sterility is variant, can be divided into 4 types complete maintenance, easily holding one by one
Type, unstable maintenance and it is difficult maintenance.Therefore maintainer screening is the key that can transformation sterile line successful.Radish
As Constantly allogamous plant, with serious inbreeding depression phenomenon, maintainer as sterile line transformation recurrent parent, with
Easily there is serious degeneration in the increase of transformation generation, the maintainer of inbreeding of more generation, influences the stabilization of sterile line, and reduction sterile line coordinates
Power.
Since 1970s, radish male sterile transformation, largely carried out using work such as the, researchs of genetic mechanism,
The application of the especially modern biotechnology such as molecular marker assisted selection promotes the optimization of maintainer screening operation, but passes through people
Work, which carries out selfing, the traditional approach of hybridization, not to be improved, and the field work of sterile line transformation is no to be optimized, therefore changes
The mode of operation for entering the field transformation work of radish sterile line is very urgent.
The content of the invention
In view of this, consume a large amount of artificial during the purpose of the present invention works for current radish male sterile seed selection
There is provided a kind of saving of labor, efficient selection for weak point.
The present invention is solved the above problems by following technological means：
A kind of method of Ogura cytoplasmic male sterility system seed selection of the present invention, comprises the following steps：
Step 1：Hybridization produces F1；
Step 1.1：Maintainer is selected；
Self-mating system of the maintainer selection with excellent character, it is desirable to which kind property is pure, and uniformity is high, without obvious inbreeding depression, from
Hand over setting percentage high；The strain of maintainer kind meets germplasm feature, grows neat and consistent；
Step 1.2：Female parent selection；
The plant with sterile character of selective nursery growth selection well is used as female parent；
Step 1.3：Plant strain field planting；
5 plants of male parent, female parent 5 involves piece field planting in a criminal case, and with other minizones every 1.5 meters；
Step 1.4：Florescence is handled；
Planted strain before florescence with insect preventing net cover to be isolated, Post flowering investigates male parent uniformity again, pulls out and does not meet unanimously
The hybrid strain of property；Female parent investigation fertility, pulls out fertile plant, all kinds of strains are deflorated after investigation is completed, and then put bumblebee or wall honeybee enters
Row pollination；
Step 1.5：Sowing；
Maintainer individual plant sowing is preserved, F1Mixed receive of cenospecies preserves；
Step 2：Hybridization produces BC1F1；
Step 2.1：Sowing；
The seed preserved in maintainer optional step 1.5, the maternal F to be preserved in step 1.51Cenospecies；
Step 2.2：Plant strain field planting；
Maintainer purity is investigated, is met after the requirement of step 1.1,10 plants of strain of screening kind；Female parent selects the strain of well-grown kind
More than 50 plants；Maintainer and maternal contiguous plant, and with other minizones every 1.5 meters；
Step 2.3：Florescence is handled；
Plant strain before blooming with insect preventing net cover to be isolated, Post flowering investigates male parent uniformity again, pulls out and does not meet unanimously
The hybrid strain of property；Female parent investigation fertility, pulls out fertile plant and calculates F1Generation sterile rate X0, all kinds of strains are deflorated after investigation is completed, so
After put bumblebee or wall honeybee is pollinated, X0If 0, maintainer is is difficult maintenance, and this maintainer is eliminated；
Step 2.4：Estimate transformation from generation to generation；
According to X0Estimate radish sterile line transformation BCnF1Sterile rate, determine transformation algebraically and estimate BC1F1Sterile rate；
Step 2.5：Sowing；
Identical with step 1.5, maintainer individual plant sowing is preserved, BC1F1Mixed receive preserves；
Step 3：Hybridization produces BC2F1；
It is identical with step 2, according to the BC of factual survey1F1Sterile rate X1It is compared with the sterile rate of estimation, works as reality
When the sterile rate of investigation is substantially less than the sterile rate of estimation, maintainer is the suspicious type of unstable maintenance, and sterile rate is by environment etc.
Influence is larger, and this maintainer is eliminated；
Step 4：Hybridization produces BC3F1；
It is identical with step 3, eliminate the maintainer of the unstable suspicious type of maintenance；BCnF1Generation and the heredity of recurrent parent maintainer
Similar componentBC3F193.75% is reached with the hereditary similar component of samsara maintainer, then Preliminary Determination is matched somebody with somebody
With joint efforts and trial cenospecies；
Step 5：Sterile line breeding is completed；
BC3F1Backcross transformation process after instead of is identical with step 4, determines transformation process according to the sterile rate of sterile line, directly
To requirement and stably is reached, then carry out follow-up breeding of new variety work.
Further, the plant with sterile character of selective nursery growth selection well is used as female parent in the step 1.2
Described in it is maternal be commodity kind F1 or stable sterile line.
Further, the male parent in the step 1.3 is maintainer.
Further, according to X in the step 2.40Estimate radish sterile line transformation BCnF1Sterile rate formula be Xn=
1-(1-X0)n+1。
A kind of method of Ogura cytoplasmic male sterility system seed selection of the present invention has the advantages that：
The purpose of the present invention is that a large amount of artificial weak points are consumed in being worked for current radish male sterile seed selection,
A kind of saving of labor, efficient selection are provided.
Had the following advantages that with seed selection Ogura cytoplasmic male sterility system of the present invention：
(1) seed selection of Ogura cytoplasmic male sterility system is the pith of radish crossbreeding, but production practices show that radish is male
Property sterile gene it is complicated, the intricate operation of maintainer screening and sterile line transformation.According to conventional male sterile line transformation means,
Substantial amounts of artificial carry out transformation work is needed, and this method is saved manually during transformation using entomophilas such as wall honeybees, is expanded
Transformation scale, can effectively exclude unstable maintenance maintainer.
(2) according to conventional male sterile line transformation means, as the maintainer of samsara parent's wood with the increasing of self-generation
Plus the phenomenon of inbreeding depression is more serious, it is unfavorable for selecting excellent male sterile line.Therefore increase is needed during transformation
The selection work of recurrent parent, the present invention carries out adding generation using the mixed mode handed over of maintainer, can Selfing avoidance decay, together
Shi Liyong insect pollinations, are reduced artificial.
Embodiment
The content of the invention of the present invention is described further with reference to embodiment.
(1) hybridization produces F1
(1.1) maintainer is selected
From self-mating system A1, A2, A3, A4 as maintainer, it is white from local varieties Yangzhou circle that four self-mating systems distinguish seed selection
It is radish (A1, Nanjing ideal seedling Co., Ltd), inside and outside green turnip (A2, the prosperous agriculture vegetable seedling breeding center in Qing County), superfine red beautiful
Loguat leaf (A3, Jiangxi Province Fengcheng City Vegetable Research Institute), Nanjing are red (A4, Nanjing Wo Shuzhong industry Co., Ltd).Maintainer character
Excellent, kind property is pure, and uniformity is high, and without obvious inbreeding depression, self-fruitful rate is high.Plant strain and meet local varieties feature, grow whole
Homogeneous is caused.
(1.2) female parent selection
The precocious red small new cherry radish of female parent selection commodity kind, is produced by Hong Kong Gao Hua seeds Co., Ltd.
(1.3) strain field planting is planted
5 plants of male parent (maintainer), female parent 5 involves piece field planting in a criminal case, and with other minizones every 1.5 meters.
(1.4) florescence is handled
Planted strain before florescence with suitable size insect preventing net cover to be isolated, Post flowering investigates male parent uniformity again, and pulling out can
Doubt hybrid strain；Female parent investigation fertility, pulls out fertile plant, all kinds of strains are deflorated after investigation is completed, and are then put bumblebee or wall honeybee etc. and are entered
Row pollination.
(1.5) sowing
Maintainer individual plant sowing is preserved, F1Mixed receive of cenospecies preserves.
(2) hybridization produces BC1F1
(2.1) sow
The seed preserved in maintainer optional step (1.5), female parent is the middle F preserved of step (1.5)1Cenospecies.
(2.2) strain field planting is planted
Maintainer purity is investigated, after the requirement for meeting step (1.1), 10 plants of strain of screening kind.Female parent selects well-grown kind
More than 50 plants of strain.Maintainer and maternal contiguous plant, and with other minizones every more than 1.5 meters.
(2.3) florescence is handled
Plant strain before blooming with suitable size insect preventing net cover to be isolated, Post flowering investigates male parent uniformity again, and pulling out can
Doubt hybrid strain；Female parent investigation fertility, pulls out fertile plant and calculates F1Generation sterile rate X0All kinds of strains are deflorated after investigation is completed, then
Bumblebee or wall honeybee etc. is put to be pollinated.X0If 0, maintainer is is difficult maintenance, and this maintainer is eliminated.Four guarantors in this example
Hold the F for being1Generation sterile rate X0Statistics such as table 1, wherein A2 maintainers are eliminated to be difficult maintenance.
The F of table 11Generation sterile rate X0Statistics
| Sterile rate | A1 | A2 | A3 | A4 |
| X0 | 33% | 0 | 85% | 95% |
(2.4) the estimation transformation generation
According to X0Estimate radish sterile line transformation BCnF1Sterile rate Xn=1- (1-X0)n+1, determine transformation algebraically and estimate
BC1F1Sterile rate.According to X in this example0Sterile rate estimation situation is as shown in table 2, wherein the transformation system energy of A3 and A4 maintainers
It is enough that 100% infertility is reached in 3 to 4 generations.
The sterile rate estimation situation of table 2
| Sterile rate | A1 | A3 | A4 |
| X1(BC1F1) | 55% | 98% | 99% |
| X2(BC2F1) | 70% | 99% | 100% |
| X3(BC3F1) | 80% | 100% | 100% |
(2.5) sowing
Identical with step (1.5), maintainer individual plant sowing is preserved, BC1F1Mixed receive preserves.
(3) hybridization produces BC2F1
It is identical with step (2), it should be noted that according to the BC of factual survey1F1Sterile rate X1With the sterile rate of estimation
It is compared, wherein the BC of A1 maintainers transformation1F1Sterile rate X1About 20%, less than 55%, maintainer is unstable maintenance
Suspicious type, sterile rate is influenceed larger by environment etc., and this maintainer is eliminated.The BC of A3 and A4 maintainer transformations1F1Sterile rate X1With
Estimated value is approached, and continues follow-up work.
(4) hybridization produces BC3F1
It is identical with step (3), eliminate the maintainer of the unstable suspicious type of maintenance.BCnF1In generation, loses with recurrent parent maintainer
Pass similar componentBC3F193.75% is reached with the hereditary similar component of samsara maintainer, being capable of just pacing
Determine coordinate force and trial cenospecies.
(5) sterile line breeding is completed
BC3F1Backcross transformation process after instead of is identical with step (4), and transformation process is determined according to the sterile rate of sterile line,
The sterile rate of sterile line of incubation is stepped up, until reaching requirement and stably, then carries out follow-up breeding of new variety work.A3
With A4 maintainers transformation to BC5F1After instead of, sterile line performance is stable, and sterile rate reaches 100%, with maintainer phenotypic character one
Cause, be identified as sterile line BYXA3001 and BYXA4001.
Finally illustrate, the above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although with reference to compared with
The present invention is described in detail good embodiment, it will be understood by those within the art that, can be to skill of the invention
Art scheme is modified or equivalent substitution, and without departing from the objective and scope of technical solution of the present invention, it all should cover at this
Among the right of invention.
Claims (1)
1. a kind of method of Ogura cytoplasmic male sterility system seed selection, it is characterised in that：Comprise the following steps：Step 1：Hybridization is produced
F1；
Step 1.1：Maintainer is selected；
Self-mating system of the maintainer selection with excellent character, it is desirable to which kind property is pure, and uniformity is high, without obvious inbreeding depression, from knot
Real rate is high；The strain of maintainer kind meets germplasm feature, grows neat and consistent；
Step 1.2：Female parent selection；
The plant with sterile character of selective nursery growth selection well is used as female parent；
Step 1.3：Plant strain field planting；
5 plants of male parent, female parent 5 involves piece field planting in a criminal case, and with other minizones every 1.5 meters；
Step 1.4：Florescence is handled；
Planted strain before florescence with insect preventing net cover to be isolated, Post flowering investigates male parent uniformity again, pulls out and does not meet uniformity
Hybrid strain；Female parent investigation fertility, pulls out fertile plant, all kinds of strains are deflorated after investigation is completed, and then put bumblebee or wall honeybee is awarded
Powder；
Step 1.5：Sowing；
Maintainer individual plant sowing is preserved, F1Mixed receive of cenospecies preserves；
Step 2：Hybridization produces BC1F1；
Step 2.1：Sowing；
The seed preserved in maintainer optional step 1.5, the maternal F to be preserved in step 1.51Cenospecies；
Step 2.2：Plant strain field planting；
Maintainer purity is investigated, is met after the requirement of step 1.1,10 plants of strain of screening kind；Female parent selects 50 plants of well-grown kind strain
More than；Maintainer and maternal contiguous plant, and with other minizones every more than 1.5 meters；
Step 2.3：Florescence is handled；
Plant strain before blooming with insect preventing net cover to be isolated, Post flowering investigates male parent uniformity again, pulls out and does not meet uniformity
Hybrid strain；Female parent investigation fertility, pulls out fertile plant and calculates F1Generation sterile rate X0, all kinds of strains are deflorated after investigation is completed, Ran Houfang
Bumblebee or wall honeybee are pollinated；X0If 0, maintainer is is difficult maintenance, and this maintainer is eliminated；
Step 2.4：Estimate transformation from generation to generation；
According to X0Estimate radish sterile line transformation BCnF1Sterile rate, determine transformation algebraically and estimate BC1F1Sterile rate；
Step 2.5：Sowing；
Identical with step 1.5, maintainer individual plant sowing is preserved, BC1F1Mixed receive preserves；
Step 3：Hybridization produces BC2F1；
It is identical with step 2, according to the BC of factual survey1F1Sterile rate X1It is compared with the sterile rate of estimation, works as factual survey
Sterile rate when being substantially less than the sterile rate of estimation, maintainer is the suspicious type of unstable maintenance, and sterile rate is influenceed by environment etc.
Larger, this maintainer is eliminated；
Step 4:Hybridization produces BC3F1；
It is identical with step 3, eliminate the maintainer of the unstable suspicious type of maintenance；BCnF1In generation, is similar to recurrent parent maintainer heredity
CompositionBC3F1Reach 93.75% with the hereditary similar component of samsara maintainer, then Preliminary Determination coordinate force and
Trial cenospecies；
Step 5:Sterile line breeding is completed；
BC3F1Backcross transformation process after instead of is identical with step 4, and transformation process is determined according to the sterile rate of sterile line, until reaching
To requiring and stably, then carry out follow-up breeding of new variety work；
The plant with sterile character of selective nursery growth selection well is as maternal for business described in female parent in the step 1.2
Kind F1 or stable sterile line；
Male parent in the step 1.3 is maintainer；
According to X in the step 2.40Estimate radish sterile line transformation BCnF1Sterile rate formula be Xn=1- (1-X0)n+1。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510662941.6A CN105284592B (en) | 2015-10-14 | 2015-10-14 | A kind of method of Ogura cytoplasmic male sterility system seed selection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510662941.6A CN105284592B (en) | 2015-10-14 | 2015-10-14 | A kind of method of Ogura cytoplasmic male sterility system seed selection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105284592A CN105284592A (en) | 2016-02-03 |
| CN105284592B true CN105284592B (en) | 2017-11-07 |
Family
ID=55183113
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510662941.6A Active CN105284592B (en) | 2015-10-14 | 2015-10-14 | A kind of method of Ogura cytoplasmic male sterility system seed selection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105284592B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108887176B (en) * | 2018-09-20 | 2020-08-07 | 中国农业科学院蔬菜花卉研究所 | Radish cytoplasmic sterile line with high seed production yield and maintainer line breeding method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103651096A (en) * | 2013-11-15 | 2014-03-26 | 南京市蔬菜科学研究所 | Breeding method of radish with purple peel |
| CN104115754A (en) * | 2014-03-14 | 2014-10-29 | 山东省农作物种质资源中心 | Radish maintainer line with Ogura male-sterile cytoplasms and breeding method for radish maintainer line with Ogura male-sterile cytoplasms |
-
2015
- 2015-10-14 CN CN201510662941.6A patent/CN105284592B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103651096A (en) * | 2013-11-15 | 2014-03-26 | 南京市蔬菜科学研究所 | Breeding method of radish with purple peel |
| CN104115754A (en) * | 2014-03-14 | 2014-10-29 | 山东省农作物种质资源中心 | Radish maintainer line with Ogura male-sterile cytoplasms and breeding method for radish maintainer line with Ogura male-sterile cytoplasms |
Non-Patent Citations (1)
| Title |
|---|
| 萝卜雄性不育系117A 的选育;胡天华等;《中国蔬菜》;20081231(第10期);摘要及第1.1、2.1节、表1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105284592A (en) | 2016-02-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2016410429B2 (en) | Method for breeding brassica napus varieties and materials with double haploid induction line of rapeseed | |
| Bruckner et al. | Self-incompatibility in passion fruit (Passiflora edulis Sims) | |
| AU2016410430B2 (en) | Method for breeding cruciferous vegetable materials and varieties with double haploid induction line of rapeseed | |
| CN104025998B (en) | Utilize the method for hybridized induction monoploid selecting and breeding corn inbred line | |
| CN106035066A (en) | Method for breeding rape interspecific and distant hybridization material by rape double haploid inducing line | |
| CN106069718A (en) | The method of Brassica campestris L dihaploid induction system selection-breeding cabbage type rape cytoplasmic male sterile line | |
| CN108377899B (en) | Breeding method for auxiliary selection of corn hybridization induced haploid by yellow-green seedling marker | |
| CN105104168B (en) | The close hybrid rice breeding method of parent's duration from seeding to heading | |
| CN109349099A (en) | Brassica napus recessive gms line is close, distant hybridization selectively breeding hybrid rape selection | |
| Chen et al. | Production of allohexaploid Brassica hybrid between tuber mustard (Brassica juncea L. var. crassicaulis Chen &Yang) and Chinese kale (Brassica oleracea var. alboglabra Bailey) | |
| CN107347625B (en) | Strong application of the female self-mating system LC-03 in pumpkin breeding | |
| CN106035068B (en) | The method of rape dihaploid induction system selection and breeding mustard type rape kind and material | |
| CN104221849A (en) | Breeding method for controlling purity of brassica napus recessive epistatic genic male sterile hybrids | |
| CN105284592B (en) | A kind of method of Ogura cytoplasmic male sterility system seed selection | |
| Bhat et al. | Identification and overcoming barriers between Brassica rapa L. em. Metzg. and B. nigra (L.) Koch crosses for the resynthesis of B. juncea (L.) Czern. | |
| CN104115754A (en) | Radish maintainer line with Ogura male-sterile cytoplasms and breeding method for radish maintainer line with Ogura male-sterile cytoplasms | |
| Khan et al. | Use of intergeneric cross for production of doubled haploid wheat (Triticum aestivum L.) | |
| CN106234207B (en) | A kind of method of Chinese cabbage cytoplasmic male sterile line selection and breeding | |
| CN105638447B (en) | Improve the selection of Rapeseed GMS system's yield and seed production purity | |
| CN106069721A (en) | Brassica campestris L dihaploid induction system's selection-breeding turnip type rape kind and the method for material | |
| JP2958457B1 (en) | Production of hybrid seeds of the first generation and production, maintenance and propagation of seed parent lines | |
| CN107484655B (en) | A kind of selection of anti-clubroot preserved sichuan pickle cytoplasm male sterility hybrid | |
| CN104303992B (en) | A kind of breeding method of high mildew-resistance Fructus Momordicae charantiae | |
| Khan et al. | Sadaf-a potential donor for enhancing frequency of doubled haploids in wheat× maize crossing system. | |
| CN108990794A (en) | A kind of hybrid rice breeding method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |