CN109452162A - A kind of quick breeding method of high cooperation rice CMS line - Google Patents

Abstract

The invention discloses a kind of quick breeding methods of high cooperation rice CMS line, this method is female parent with rice recessive Genetic Sterility material YNS, it is respectively that restorer hybridizes, is selfed with multiple conventional rices three, the sterile plant close with restorer character to isolate obtains stable and almost the same with restorer character sterile line for maternal continuation and restorer backcrossing, selfing；It based on this, is female parent using these sterile lines, the relatively stable holding with breeding is strain test cross, and according to the comprehensive agronomy character of test cross F1, the holding for filtering out high-combining ability is strain, the corresponding CMS line of further breeding.The method of the present invention can measure in advance and screen the coordinate force of CMS line, and the holding for rejecting coordinate force difference is strain, effectively the CMS line of breeding high-combining ability, reduce breeding cost and risk.

Description

A kind of quick breeding method of high cooperation rice CMS line

Technical field

The invention belongs to crop breeding fields, are related to a kind of quick breeding method of high cooperation rice CMS line.

Background technique

The research and application of series of three-series hybrid rice greatly advance the development of China's rice breeding cause, CMS line Breeding be the key that series of three-series hybrid rice application, high-combining ability sterile line can generate more cross combinations, Exploitative potential Greatly.The CMS line breeding period is long, and heavy workload, blindness is strong, and the sterile line finally cultivated may be poor due to coordinate force The disadvantages of cannot be promoted and applied in production；Therefore, how efficiently breeding CMS line is particularly important.

Recessive cytoblast sterile material discovery is more early, and Genetic Mechanisms are simple, but Breeding Application is less.The present invention passes through field nature Variation one plant of recessive cytoblast sterile material YNS of discovery, the stigma exposing ratio of sterile line is high, is selfed shaky and solid not by temperature Regulation, with multiple three be restorer and holding is that combo F1 generation is fertile, and F2 meets 3:1 with sterile single plant and separate for fertile Than illustrating that the infertility character is controlled by single recessive nuclear gene.Therefore, the characteristic of YNS can be utilized, it is good that quick breeding goes out coordinate force CMS line plant, workload and production cost can be greatly reduced, have certain economic significance and social effect.

Summary of the invention

The present invention provides a kind of quick breeding methods of high cooperation rice CMS line, to overcome traditional breeding height to match Close the problems such as CMS line purpose is not strong, blindness is big, time-consuming and laborious.

The present invention through the following technical solutions to achieve the above objectives:

A kind of quick breeding method of high cooperation rice CMS line, step include:

It is female parent with stealthy Genetic Sterility material YNS, selecting a variety of conventional rices three is restorer for male parent, is carried out respectively miscellaneous It hands over, filial generation selects pollen sterility and the single plant close with male parent economical character is female parent, be returned with male parent after being selfed, Backcross progeny is selfed again, selects pollen sterility and the single plant close with male parent economical character, after being repeatedly returned and be selfed, again Backcrossing, until building obtains and the consistent a variety of pollen sterility systems introgressive line of male parent economical character when BCmF1；

Pollen sterility system introgressive line is separated through selfing, obtains a variety of sterile single plants；

The coordinate force for keeping system to breeding rice varieties using above-mentioned a variety of sterile single plant measurements, eliminates the guarantor of coordinate force difference Holding is strain, and conventional method breeding is recycled to obtain sterile line.

Further, the conventional rice three be restorer kind include Jiangxi extensive 752, Zhenhui 084, bright extensive 86, another name for Sichuan Province is extensive 527, Cheng Hui 177, Luhui 615, Hua Hui 2, first extensive 207, Mianhui725, Yanhui 559, Restorer line Yihui1577, Shen Hui 254, Anhui are extensive 3404, Rong is extensive 906, interior extensive 182, Chang Hui 117, bright extensive 100, Chenghui 047, Lu are extensive 602,20, extensive No. 9 of connection etc..

Further, it is returned 5 times using the stealth Genetic Sterility material YNS, is i.e. when m=5, obtains stable pollen sterility It is introgressive line.

Further, the store method of pollen sterility system introgressive line are as follows: by BCnF1 generation selfing mixed pollination, receive The Seed storage in sterile plant is taken, wherein n > m.

Further, the combining ability test method of system is kept to breeding rice varieties, step includes: with a variety of sterile single plants It is that male parent carries out test cross respectively to keep system to breeding rice varieties, the F1 generation of acquisition measures coordinate force for female parent.

Further, the stealth Genetic Sterility material YNS is saved by rice stub.

The beneficial effects of the present invention are: for the present invention by utilizing recessive cytoblast sterile material YNS, it is extensive for constructing a set of three Again it is introgressive line, is that restorer introgressive line is selfed the sterile single plant separated using three, eliminates the holding system strain of a large amount of coordinate force differences System, and then high-combining ability CMS line is selected, purpose is strong, save the cost, and efficiency of selection is high, can be more acurrate, quick The CMS line of suitable production requirement is cultivated on ground, reduces experimentation cost, simplifies mode of operation, effectively facilitates production effect Rate.

Detailed description of the invention

Fig. 1 is the breeding technique route map of CMS line rice.

Specific embodiment

Embodiment 1

(1) test material

The present embodiment method therefor is known to those skilled in the art the conventional method of dawn unless otherwise instructed, used The materials such as reagent be commercially available products unless otherwise instructed.Used rice varieties are unless otherwise noted Conventional commercial kind.

(2) experimental method

As shown in Figure 1, present embodiments providing a kind of quick breeding method of Three-line rice sterile line, step includes water It is coordinate force screening and three systems of rice that the acquisition of rice sterile line introgressive line, stable rice, which keep the breeding of system, stable holding, The breeding of sterile line rice, the specific steps are as follows:

1) sterile line introgressive line BC5F1 is obtained

I) acquisition of YNS: the Hefei ,Anhui experimental plot where the rice of Academy of Agri-Science and Technology Anhui Province finds one plant of nothing within 1998 Pollen and the high recessive cytoblast sterile material of stigma exposing ratio, are named as YNS, are female parent with it, are restorer and guarantor with multiple three Holding is combo, and F1 generation is fertile, and F2 meets 3:1 segregation ratio with sterile single plant for fertile, and sterile plant pollen fertility is not by temperature tune Control continues abortion, illustrates that YNS infertility character is controlled by single recessive nuclear gene.The YNS is at present by Academy of Agri-Science and Technology Anhui Province water The preservation of rice research institute, using rice stub, summer is saved in Hefei, winter in Hainan or in the incubator.

Ii) the acquisition of sterile line introgressive line BC5F1: the YNS that positive season in 2000 is saved using rice stub is extensive 752 with Jiangxi respectively, Zhenhui 084, bright extensive 86, another name for Sichuan Province is extensive 527, Cheng Hui 177, Luhui 615, Hua Hui 2, first extensive 207, it is Mianhui725, Yanhui 559, preferably extensive 1577, Shen Hui 254, Anhui is extensive 3404, Rong is extensive 906, interior extensive 182, Chang Hui 117, bright extensive 100, Chenghui 047, Lu are extensive 602, connection extensive No. 9 It is restorer hybridization Deng 20 three, obtains fertile Offspring F1, filial generation F1 selects economical character close to recovery after being selfed The sterile plant F2 of system, with male parent be returned fertile offspring BC1F1, backcross progeny are repeatedly selfed again, are returned, until Winter Hainan in 2005 is obtained with the almost the same BC5F1 of paternal character for introgressive line, each introgressive line grain weight be 500 with On, it saves backup, pollen sterility system introgressive line is separated through selfing, obtain 20 kinds of sterile single plant BC5F2.

2) maintainer plant F6 is stablized in breeding

Spring in 2003 is female parent using association blueness morning B in Hainan Lingshui, and early Xian 14 is male parent, is hybridized, and harvests 38 Cenospecies；2003 Nian Zhengji plant 18 plants of F1 generation in Hefei, and heading stage reestablishes diplomatic relations with suitable perfume 1B, harvests 29 cenospecies； Winter in 2003 reestablishes diplomatic relations 18 plants of F1 generation in the plantation of Hainan Lingshui, mixed to receive portion's seed；2004 Nian Zhengji plant F2 group in Hefei 2000 plants, economical character screens fine individual plant 64；2006 Nian Zhengji of winter-in 2004, in 1 year 2 generation, is sieved by economical character Choosing and the pedigree method breeding of rice quality detection meet breeding objective and relatively stable F6 generation keeps being strain 25.

3) holding of screening high-combining ability is strain

I) winter in 2006 is in Hainan Lingshui plantation step 2) obtain 25 keep being strain and the building of 20 steps 1) Using recessive cytoblast sterile material YNS and three be restorer building BC5F1 for introgressive line；BC5F1 generation selfing is isolated Sterile single plant BC5F2 is female parent, stablizes with 25 keep being strain F6 test cross respectively, amounts to 20 introgressive line *, 25 holding systems Strain=500 combination, each combination harvest F1 generation cenospecies 20 or more；

Ii) 2007 Nian Zhengji plant test cross F1 in Hefei, and 2 rows of each combination, 9 plants of every row, observation economical character, interior are examined Kind, rice quality detection, finishing screen select comprehensive agronomy character and good grain quality, coordinate force and preferably keep being strain 1, be named as Emblem 12B.

4) breeding three-line sterile line strain

Winter in 2007, emblem 12B was recurrent parent in Hainan Lingshui using Xieqingzao A as donor parents, and hybridization is returned, often A generation plants sterile line in pairs and holding is male parent, until winter in 2011 obtains BCnF1 for stable sterile line, is named as emblem 12A, while obtaining stable holding is emblem 12B.

Winter in 2012 is right using emblem 12A and free restorer progress test cross more than 50 in Hainan Lingshui, and 2013 Nian Zhengji exist Hybrid vigour identification is planted and is carried out in Hefei, is matched combination and is shown that plant height, ripe phase are moderate, and fringe type is larger, setting percentage is high, resists Property the strong, features such as rice matter is more excellent；Wherein there are 9 combinations 5% or more compared with control excellent 838 amount of increase in production of II, is transplanting per acre Under the conditions of 20000 caves, panicle number per plant 8.4~12.2, every total grain panicle number 155.3~203.0, setting percentage 80.1%~ 86.9%, 28.0~32.2g of mass of 1000 kernel, 651.2~692.0kg/ mus of yield, than excellent 838 amplification of control II 5.5%~ 12.1%, meter Zhi Jun issue third edible rice grade or more up to portion.

2016 years Anhui Province is participated in using emblem 12A and excellent 165 (the emblem 12A/R165) in the matched cross combination emblem restorer R165 The test of Hui Dao association, average product are 672.4kg/ mus, and 838 volume increase more excellent than the check variety II portion 6.7%, meter Zhi Wei issues three Deng；The continuous examination of Hui Dao association, Anhui Province in 2017 is participated in, average product is 658.4kg/ mus, 838 volume increase more excellent than check variety II Issue third in the portion 5.3%, meter Zhi Wei.

The above are a kind of detailed embodiment and specific operating process of the present invention, are before being with technical solution of the present invention It puts and is implemented, but protection scope of the present invention is not limited to the above embodiments.

Claims (6)

1. a kind of quick breeding method of high cooperation rice CMS line, which is characterized in that step includes:

It is female parent with stealthy Genetic Sterility material YNS, selecting a variety of conventional rices three is restorer for male parent, hybridized respectively, Filial generation selects pollen sterility and the single plant close with male parent economical character is female parent, be returned with male parent, backcrossing after being selfed Offspring is selfed again, selects pollen sterility and the single plant close with male parent economical character returns again after being repeatedly returned and be selfed It hands over, until building obtains and the consistent a variety of pollen sterility systems introgressive line of male parent economical character when BCmF1；

Pollen sterility system introgressive line is separated through selfing, obtains a variety of sterile single plants；

The coordinate force for keeping system to breeding rice varieties using above-mentioned a variety of sterile single plant measurements, eliminates the holding system of coordinate force difference Strain recycles conventional method breeding to obtain sterile line.

2. a kind of quick breeding method of high cooperation rice CMS line according to claim 1, which is characterized in that institute It includes that Jiangxi extensive 752, Zhenhui 084, bright extensive 86, another name for Sichuan Province is extensive 527, Cheng Hui 177, Luhui 615, China that state conventional rice three, which be restorer kind, Extensive No. 2, first extensive 207, Mianhui725, Yanhui 559, Restorer line Yihui1577, Shen Hui 254, Anhui is extensive 3404, Rong is extensive 906, interior extensive 182, Chang Hui 117, bright extensive 100,20, extensive 602, extensive No. 9 of connection in Chenghui 047, Lu etc..

3. a kind of quick breeding method of high cooperation rice CMS line according to claim 1, which is characterized in that benefit It is returned 5 times with stealthy Genetic Sterility material YNS, is i.e. when m=5, obtains stable pollen sterility system introgressive line.

4. a kind of quick breeding method of high cooperation rice CMS line according to claim 1, which is characterized in that institute State the store method of pollen sterility system introgressive line are as follows: by BCnF1 generation selfing mixed pollination, the seed collected in sterile plant is protected It deposits, wherein n > m.

5. a kind of quick breeding method of high cooperation rice CMS line according to claim 1, which is characterized in that Breeding rice kind keeps the combining ability test method of system, and step includes: with a variety of sterile single plants for female parent, to breeding rice It is that male parent carries out test cross respectively that kind, which keeps system, and the F1 generation of acquisition measures coordinate force.

6. a kind of quick breeding method of high cooperation rice CMS line according to claim 1, which is characterized in that institute Stealthy Genetic Sterility material YNS is stated to save by rice stub.