CN1561692A - Method for preparing two-line hybrid rice seeds by utilizing ecological male sterility - Google Patents
Method for preparing two-line hybrid rice seeds by utilizing ecological male sterility Download PDFInfo
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- CN1561692A CN1561692A CN 200410012243 CN200410012243A CN1561692A CN 1561692 A CN1561692 A CN 1561692A CN 200410012243 CN200410012243 CN 200410012243 CN 200410012243 A CN200410012243 A CN 200410012243A CN 1561692 A CN1561692 A CN 1561692A
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 55
- 235000009566 rice Nutrition 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 13
- 206010021929 Infertility male Diseases 0.000 title abstract 3
- 208000007466 Male Infertility Diseases 0.000 title abstract 3
- 240000007594 Oryza sativa Species 0.000 title description 3
- 241000209094 Oryza Species 0.000 claims abstract description 52
- 238000004519 manufacturing process Methods 0.000 claims abstract description 16
- 230000035558 fertility Effects 0.000 claims abstract description 9
- 238000009331 sowing Methods 0.000 claims description 8
- 244000037666 field crops Species 0.000 claims description 6
- 230000008774 maternal effect Effects 0.000 claims description 6
- 230000001488 breeding effect Effects 0.000 claims description 5
- 238000009395 breeding Methods 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 claims description 3
- 230000012010 growth Effects 0.000 claims description 3
- 238000009396 hybridization Methods 0.000 abstract description 8
- 230000007547 defect Effects 0.000 abstract 2
- 241000196324 Embryophyta Species 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000009401 outcrossing Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention relates to a seed production method of hybrid rice, in particular to a method for preparing two-line hybrid rice seeds by utilizing ecological male sterility. The method comprises the process steps of primary selection of hybrid parents, selection of two-line hybrid rice combination, production of two-line hybrid rice seeds and the like, and solves the defects of less parent resources, less combination, low probability of selecting excellent combination and the like in the prior art. The conventional variety is utilized to cultivate the two-line hybrid rice, the male sterility of the female parent can be conveniently realized through technical measures such as light-temperature regulation and control, the fertility of the male parent is normal, pollen is provided for the female parent, the japonica-japonica hybridization can be realized, the japonica-indica hybridization and the indica-indica hybridization can be realized, and the rice is hybridized with the dry rice, so that the defects of few three-line or two-line hybridization combinations and weak heterosis in the north are overcome, the seed selection period is greatly shortened, the conventional variety is utilized with more resources, and the problems of strong drought resistance, high yield, poor rice quality, high yield of the three-line hybrid rice and poor rice quality of the dry rice can be solved.
Description
Technical field
The present invention relates to the producing method for seed of hybrid rice, be meant a kind of method of utilizing ecological male sterile to prepare the double-line hybrid rice especially.
Background technology
(Shi Mingsong, late round-grained rice is dual-purpose naturally to be seed selection and application preliminary study, hubei agricultural science, 1981 (7): 1-3) reported that the dual-purpose system of the parent of transformation and discovery emerges in an endless stream since the dual-purpose naturally system of late round-grained rice from the bright pine of stone in 1981.At present dual-purpose is photosensitive and temperature-sensitive sterile line, the prime strain of these materials is from land for growing field crops selfing mutant, other are many, and (Zhou Guang is in harmony, the light temperature of temperature sensitive nuclear sterile rice ecological from hybridization, publishing house of Hunan Normal University, 1996,12:44-57), China northeast Liaoning (Li Jikai etc., Rice Population breeding theory and practice, cultivation and rice do, 2004 (1): 3-6) report, dual-purpose system is introduced by the other places before this, and is back with conventional incross seed selection, the male sterile phenomenon that other people find is a kind of phenomenon, is that the minority kind has.Existing double-linear hybrid rice is under general condition cultivated, shortcoming such as above prior art exists that parent's resource is few, the probability of combined few, selection fine combination is low.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing ecological male sterile to prepare the double-line hybrid rice to overcome the deficiency that prior art exists.
Overall technology design of the present invention is:
Utilize ecological male sterile to prepare the method for double-line hybrid rice, it comprises following processing step:
(1) primary election of hybrid strain
Select different conventional varieties for use, transplant by sowing the same period same period respectively, interval sowing is transplanted by stages, the same period, sowing was transplanted by stages, the mode of the interval sowing transplanting same period, in conjunction with light modulation, the temperature adjustment measure, make it in different ecotope growths, determine same kind sterile under different ecological condition and can educate the period, the kind of selecting flower synchronization in a certain amount of time is as the parent, wherein male sterile as maternal, fertility is normally as male parent, thereby select experiment with hybrid rice production of hybrid seeds parent, and determine that same kind is respectively as the ecotope of female parent or male parent;
(2) selection of two-line hybrid rice combination
Normal according to maternal male sterile, male parent fertility, the condition of the two flower synchronization, the parent cultivates under identical or different ecological condition with the hybrid rice production of hybrid seeds, and preparation two-line hybrid rice F1 seed is used to carry out name by government behind strain comparison, the regional experiment; Select the good hybrid combination of proterties and be used for the hybrid rice seed that the production land for growing field crops is used as the parent;
(3) production of double-line hybrid rice
With the good hybrid combination that obtains in (2) step as the parent, utilize determine in (1) step with male sterile as female parent, fertility normally as male parent, and the ecotope of flower synchronization, the parent is cultivated, obtain the double-line hybrid rice and be used for land for growing field crops production.
Specific structural features of the present invention also has:
The parental seed that is used for the hybrid rice production of hybrid seeds in step (1), (2), (3) is under normal production conditions, produces by breeding breeding program to obtain.
Light modulation, temperature adjustment measure are color, the adjustment overlay film time of shed, greenhouse, overlay film, adjusting film.
The obtained technological progress of the present invention is:
Adopt method of the present invention to utilize conventional variety to cultivate two-line hybrid rice, can make maternal male sterile by technical measures such as light temperature regulation and control easily, the male parent fertility is normal, for female parent provides pollen, not only can realize the hybridization of round-grained rice round-grained rice, all right round-grained rice Xian, Xian Xian hybridization, paddy rice and upland rice hybridization, thereby overcome the north three be or the double-line hybrid combined few, the shortcoming that hybrid vigour is not strong, shortened simultaneously the cycle of seed selection greatly, utilized the conventional variety resource many, it is strong and output is not high to be used to solve the upland rice drought resistance, bad and the ternary hybrid rice output height of rice matter, the bad problem of rice matter.
Embodiment
Below in conjunction with accompanying drawing embodiments of the invention are described further:
Present embodiment is selected Ji round-grained rice 14 for use, the Ji glutinous No. 1 respectively as female parent and male parent, the two was all sowed July 1, Ji round-grained rice 14 is reserved delegation every 6 row, place male parent when being used to ear, Ji round-grained rice 14 is cultivated under field conditions (factors) and is male sterile, glutinous No. 1 growth under field conditions (factors) in Ji, and it is normal to adopt the mode of overlay film insulation to make it fertility at night behind the jointing, potted plant mode is adopted glutinous No. 1 in the Ji, during heading it is moved into maternal district, for female parent provides pollen, outcrossing, produce the double-line hybrid seed, after the flowering, to father and mother originally all adopt platform night overlay film mode be incubated, make the hybrid seed normal mature.When next year producing in the land for growing field crops and check variety carry out strain relatively, with outstanding as produce with kind, what proterties was abominable eliminates.
Claims (3)
1, utilize ecological male sterile to prepare the method for double-line hybrid rice, it is characterized in that it comprises following processing step:
(1) primary election of hybrid strain
Select different conventional varieties for use, transplant by sowing the same period same period respectively, interval sowing is transplanted by stages, the same period, sowing was transplanted by stages, the mode of the interval sowing transplanting same period, in conjunction with light modulation, the temperature adjustment measure, make it in different ecotope growths, determine same kind sterile under different ecological condition and can educate the period, the kind of selecting flower synchronization in a certain amount of time is as the parent, wherein male sterile as maternal, fertility is normally as male parent, thereby select experiment with hybrid rice production of hybrid seeds parent, and determine that same kind is respectively as the ecotope of female parent or male parent;
(2) selection of two-line hybrid rice combination
Normal according to maternal male sterile, male parent fertility, the condition of the two flower synchronization, the parent cultivates under identical or different ecological condition with the hybrid rice production of hybrid seeds, and preparation two-line hybrid rice F1 seed is used to carry out name by government after strain comparison, the regional test; Select the good hybrid combination of proterties and be used for the hybrid rice seed that the production land for growing field crops is used as the parent;
(3) production of double-line hybrid rice
With the good hybrid combination that obtains in (2) step as the parent, utilize determine in (1) step with male sterile as female parent, fertility normally as male parent, and the ecotope of flower synchronization, the parent is cultivated, obtain the double-line hybrid rice and be used for land for growing field crops production.
2, the ecological male sterile of utilization according to claim 1 prepares the method for double-line hybrid rice, the parental seed that it is characterized in that being used in step (1), (2), (3) the hybrid rice production of hybrid seeds is under normal production conditions, produces by breeding breeding program to obtain.
3, the ecological male sterile of utilization according to claim 1 prepares the method for double-line hybrid rice, it is characterized in that described light modulation, temperature adjustment measure are color, the adjustment overlay film time of shed, greenhouse, overlay film, adjusting film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410012243 CN1561692A (en) | 2004-04-20 | 2004-04-20 | Method for preparing two-line hybrid rice seeds by utilizing ecological male sterility |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410012243 CN1561692A (en) | 2004-04-20 | 2004-04-20 | Method for preparing two-line hybrid rice seeds by utilizing ecological male sterility |
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| CN 200410012243 Pending CN1561692A (en) | 2004-04-20 | 2004-04-20 | Method for preparing two-line hybrid rice seeds by utilizing ecological male sterility |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103843555A (en) * | 2013-08-27 | 2014-06-11 | 肖卓丽 | High-yield and purity-maintaining seed production technique of two-line hybrid rice |
| CN106069453A (en) * | 2016-07-19 | 2016-11-09 | 莆田秀屿区群韬农业技术开发有限公司 | The breeding method of a kind of drought resisting rice and implantation methods |
| CN109089873A (en) * | 2018-08-03 | 2018-12-28 | 武汉大学 | Obtain the method that can be used for the Parents In Hybrid Rice combination of the same period live streaming production of hybrid seeds |
-
2004
- 2004-04-20 CN CN 200410012243 patent/CN1561692A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103843555A (en) * | 2013-08-27 | 2014-06-11 | 肖卓丽 | High-yield and purity-maintaining seed production technique of two-line hybrid rice |
| CN106069453A (en) * | 2016-07-19 | 2016-11-09 | 莆田秀屿区群韬农业技术开发有限公司 | The breeding method of a kind of drought resisting rice and implantation methods |
| CN106069453B (en) * | 2016-07-19 | 2019-06-04 | 新昌县大船畈生物科技有限公司 | A kind of breeding method and implantation methods of drought resisting rice |
| CN109089873A (en) * | 2018-08-03 | 2018-12-28 | 武汉大学 | Obtain the method that can be used for the Parents In Hybrid Rice combination of the same period live streaming production of hybrid seeds |
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