CN114532216B - Breeding method of multi-marker dual-purpose hybrid rice sterile line - Google Patents
Breeding method of multi-marker dual-purpose hybrid rice sterile line Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/022—Genic fertility modification, e.g. apomixis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/021—Methods of breeding using interspecific crosses, i.e. interspecies crosses
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Abstract
Hair brushThe breeding process of hybrid rice sterile line includes the following steps: zhejiang Huang Caihe as female parent and Zhejiang pink rice as male parent, hybridized F 1 Crossing with Zheda 01S as female parent, backcrossing with Zheda 01S, selfing, and harvesting BC n F 2 Seed generation; direct broadcast BC n F 2 Selecting a male sterile plant with pollen abortion, column head and colored perillama tip when blooming; BC n F 3～5 Selecting the seeds of the sterile line plants, and reserving the sterile line with good flowering habit; testing and matching with a restorer line, and selecting and reserving a sterile line with strong hybrid vigor; performing a seed production test, and selecting and reserving a sterile line which is easy to produce seeds; selecting and remaining sterile line with good ornamental value, finally breeding multi-mark dual-purpose hybrid rice sterile line. The dual-purpose sterile line obtained by the breeding method not only can prepare high-quality high-yield disease-resistant hybrid rice, but also can economically, efficiently and conveniently remove impurities in seed production.
Description
Technical Field
The invention relates to the technical field of rice breeding, in particular to a breeding method of a multi-marker dual-purpose hybrid rice sterile line.
Background
The hybrid rice in China provides important guarantee for national grain safety. According to popularization and statistics of main crops in China, the total planting area of the hybrid rice in 2019 is about 1.62 hundred million mu. The seed production cost of the hybrid rice is high, the procedure is complicated, the bottleneck of the deep development of the hybrid rice is formed, and particularly under the background that the labor force in the rural area is greatly transferred and the traditional seed production is more labor-consuming, the breeding of the hybrid rice sterile line which accords with the simplified cultivation or artificial intelligence and the new combination thereof is one of the future development directions of the hybrid rice seed production technology.
The combination of the leaf color mutation and the golden glume with the sterile line is used as a marker character, so that the simple impurity removal is convenient in the hybrid rice seed production. Therefore, marked sterile lines such as whole dragon A, bai Feng A, rabbit S, NHR S, marked 1S and the like, namely greening turning albino, yellow leaves, zebra leaves, purple leaves and the like are selected and bred in China successively.
In addition, due to the expansion of agricultural functions, sightseeing agriculture and ecological agriculture are developed successively, and special new varieties of colored rice, such as Huang Caihe, pink rice and Huang Caihe, are bred.
Disclosure of Invention
The invention provides a breeding method of a multi-marker dual-purpose hybrid rice sterile line, the dual-purpose sterile line obtained by the breeding method not only can breed high-quality high-yield disease-resistant hybrid rice, but also can economically, efficiently and conveniently remove impurities in the seed production, and particularly meets the light and simplified production requirements of labor saving and cost saving at present.
The specific technical scheme is as follows:
a breeding method of a multi-marker dual-purpose hybrid rice sterile line comprises the following steps:
(1) Crossing with Zhejiang Huang Caihe as female parent and Zhejiang pink rice as male parent to obtain F 1 Then with F 1 The generation is used as male parent, zheda 01S is used as female parent for hybridization, and the obtained filial generation is backcrossed with the female parent Zheda 01S for n times to obtain BC n F 1 Generation, BC n F 1 After generation selfing, harvesting BC n F 2 Seed generation;
(2) Planting BC n F 2 Directly seeding and raising rice seedlings, selecting yellow plants when seedlings have 4-5 leaves, transplanting the yellow plants to a field, selecting and reserving pollen abortive and male sterile plants with colored stigmas and palea tips during flowering, cutting and regenerating the stems, selecting and obtaining a sterile line from which fertility is converted into fertility, and selecting and reserving a sterile line S with purple stigmas and red palea tips 1 ；
(3) Planting BC n F 3～5 Identifying stigma exsertion rate and flowering habit by using purple stigma as a mark, identifying fertility of sterile line by using Zhejiang 01S as a contrast, and selecting sterile line S with good flowering habit 2 ；
(4) Planting BC n Performing indoor and outdoor sterility identification for F6 generation or more, and selecting sterile line S with sterility meeting standard requirement based on NY/T215-2006 issued by Ministry 3 Testing and matching with restorer to obtain hybrid F 1 ；
(5) For the obtained sterile line S 3 Identification of F 1 Selecting and reserving sterile line S with strong hybrid vigor 4 ；
(6) For the obtained sterile line S 4 Performing seed production test, selecting and reserving sterile line S easy for seed production 5 ；
(7) For the obtained sterile line S 5 Planting the variety in combination with the promoted variety, and selecting and reserving sterile line S with good ornamental value 6 ；
(8) Construction of multiple trait marker sterile line S 6 The restoring line and the hybrid rice system prepared by the restoring line are finally bred into the multi-mark dual-purpose hybrid rice sterile line S with yellow leaves, purple stigmas and red lemma tips 7 。
In the step (1), the Zhejiang Huang Caihe is a special color rice variety suitable for creative agricultural development and bred in Zhejiang university cooperation, and new plant variety right protection is applied to the agricultural rural department (application number: 20191005262).
The Zhejiang pink rice is a special variety of colored rice suitable for creative agricultural development and bred cooperatively by Zhejiang university. The variety has been approved by the crop variety approval committee in Zhejiang province in 2019 (Zhejiang examined rice 20190009), and the Ministry of agricultural village is applied for the right protection of new plant varieties (application No.: 20191002149).
Zhejiang 01S is a indica rice temperature-sensitive sterile line bred at Zhejiang university. The variety has been approved by agricultural and article variety approval committee in Zhejiang province in 2020 (Zhejiang examined rice [ sterile line ] 2020008), and application for new plant variety protection is applied to the agricultural rural department (application number: 20191000666).
Further, in the step (1), n is more than or equal to 3.
Further, in the step (3), the judgment criteria of good flowering habit are that the stigma exposure rate is more than or equal to 50%, and the flowering time in sunny days is concentrated at 10:30 to 11.
Further, in step (5), the heterosis evaluation index includes: the yield, the rice quality and the disease resistance, the specific indexes require that the yield is more than or equal to 600 kg/mu, the rice quality reaches the ministry-issued three-level and above standards, and the rice blast resistance is achieved.
Further, in the step (6), the easy propagation seed production has the characteristics that the stigma exposure rate is more than or equal to 50%, the outcrossing seed setting rate is more than or equal to 30%, and the seed production yield is more than or equal to 180kg.
Further, in the step (7), the popularization variety is Xiushui 134.
Further, in the step (7), the ornamental value is similar to or longer than that of the parent, the plant height is similar to or higher than that of the parent, and the lodging resistance is stronger.
Compared with the prior art, the invention has the following beneficial effects:
(1) The multi-marker dual-purpose hybrid rice sterile line bred by the invention can be used for selecting and breeding new hybrid rice varieties with high yield, high quality and disease resistance, and can also be used for creative agriculture combining agriculture and tourism.
(2) Compared with the conventional sterile line marked by single leaf color such as white to green, purple, yellow leaf and the like, the sterile line of the multi-marker dual-purpose hybrid rice obtained by the invention provides technical support for multi-character fidelity and counterfeit removal in different stages by using multiple marking characters such as yellow leaf, purple stigma, red mango with short top and the like, is beneficial to purification and rejuvenation of the sterile line and ensures production of high-quality and high-quality hybrid rice seeds.
(3) The breeding method of the multi-marker dual-purpose hybrid rice sterile line combines a plurality of visual identifiable characteristics, has unique breeding design, not only has higher and more accurate breeding efficiency, but also has economic and efficient leaves: when seedlings are raised, directly seeding yellow leaves to raise seedlings BC n F 2 When seedlings are transplanted to the field, yellow leaf marked plants are selected, so that the population meeting the breeding target is simply, conveniently and efficiently reduced; when in flowering, the plants aborted pollen are combined with the color of the stigmas and the palea tips, and are accurately selected to a target sterile line, and in addition, the clear and visible purple stigmas are used as marks to observe whether the flowering habit is good or not, so that the easy seed production of the sterile line is realized, and the foundation is laid for the economic and efficient production of hybrid rice seeds.
(4) The invention adopts the strategy of requiring backcross for 3 times or more, thus the good comprehensive character of the sterile line Zheda 01S can be kept as far as possible, and the invention lays the foundation for preparing series of multi-marker dual-purpose sterile lines.
Detailed Description
The present invention will be further described with reference to the following specific examples, which are only illustrative of the present invention, but the scope of the present invention is not limited thereto.
The breeding method provided in the following examples is a breeding method of a multi-marker dual-purpose hybrid rice sterile line based on a high-quality eurytopic hybrid rice sterile line Zheda 01S.
Wherein Zhejiang 01S is a indica rice temperature-sensitive sterile line bred at Zhejiang university. The variety is approved by the crop variety approval committee of Zhejiang province in 2020 (approval certificate: zhejiang examined rice [ sterile line ] 2020008), and applies for the protection of new plant variety right to agricultural rural departments (application number: 20191000666), and can be purchased from research institute of atomic nucleus agriculture science of Zhejiang university.
The Pingcheng rice is a special variety of colored rice suitable for creative agricultural development in Zhejiang university cooperative breeding. The variety is approved by the crop variety approval committee in Zhejiang province in 2019 (approval certificate: zhejiang approved rice 20190009), and applies for plant new variety right protection to agricultural rural departments (application number: 20191002149), and can be purchased from the institute of atomic agriculture science of Zhejiang university.
Zhejiang Huang Caihe is a special variety of colored rice suitable for creative agricultural development bred by Zhejiang university, has applied for plant new variety right protection (application number: 20191005262) to the department of agricultural village, and can be purchased from institute of nuclear agriculture department of Zhejiang university.
Huazhan is a restorer line (variety weight number CNA 20080059.0) which is prepared by taking SC02-S6 from Malaysia as a basic material and performing repeated test cross and systematic breeding for Chinese rice research. More than 100 new hybrid rice varieties with high yield, high quality and lodging resistance, such as Tianyou Huazhan, are bred by taking the hybrid rice as a restorer line, and can be purchased in the Chinese rice institute or the seed market.
Xiushui 134 is a single-season conventional late japonica rice bred by agriculture science research institute in Jiaxing city and other units, issued by Ministry of rice quality and second class, approved by Zhejiang province in 2010 (approval certificate number is Zhejiang examined rice 2010003), is a main cultivar currently produced and popularized in Zhejiang province and a control cultivar for regional tests, and can be purchased in agriculture science research institute in Jiaxing city or seed market.
Example 1
A breeding method of a multi-marker dual-purpose hybrid rice sterile line comprises the following specific steps:
(1) Crossing with Zhejiang Huang Caihe as female parent and Zhejiang pink rice as male parent to obtain F 1 Then with F 1 The generation is male parent and Zheda 01S is female parentThe obtained filial generation is backcrossed with mother plant Zheda 01S for 4 times to obtain BC 4 F 1 Generation of 15 seeds, BC 4 F 1 After generation selfing, BC is harvested n F 2 2600 seeds are used;
(2) Planting BC 4 F 2 Directly sowing and raising seedling, when 4-5 leaves of seedling are selected 152 yellow plants, transplanting them into field, when the seedling is bloomed, selecting 39 male sterile plants whose pollen is abortive and whose stigma and husk tip are coloured, cutting pile and regenerating, selecting sterile line whose fertility is transferred into fertility from them, selecting sterile line whose stigma is purple and husk tip is red, selecting sterile line S 1 26, the number of the channels is 26;
(3) Planting BC 4 F 3～5 Identifying stigma exsertion rate and flowering habit by using purple stigma as a mark, identifying fertility of sterile line by using Zhejiang 01S as a contrast, and selecting sterile line S with good flowering habit 2 12, the number of the channels is 12;
(4) Planting BC 4 F 6 And (4) identifying the sterility indoors and outdoors by taking the Ministry issued NY/T215-2006 as a standard, and selecting and reserving the sterile line S with the sterility meeting the standard requirement 3 9, and mixing with restorer line Huazhan to obtain hybrid F 1 ；
(5) For the obtained sterile line S 3 Identification of F 1 The heterosis of the rice is determined by selecting and reserving the sterile line S with strong hybrid vigor by taking the yield of more than or equal to 600 kg/mu, the rice quality reaching the national third level and above and the rice blast resistance as the judgment standard of the heterosis 4 6, the number of the cells is 6;
(6) For the obtained sterile line S 4 Performing seed production test, selecting and reserving sterile line S easy for seed production by taking stigma exsertion rate of more than or equal to 50%, outcrossing seed setting rate of more than or equal to 30% and seed reproduction and production yield of more than or equal to 180kg as selection standards 5 5, the number of the cells is 5;
(7) For the obtained sterile line S 5 Matching with Xishui 134 to make rice field painting for creative agriculture, and selecting sterile line S with good appreciation property 6 3, the number of the cells is 3;
(8) Construction of multiple trait marker sterile line S 6 The restoring line and the hybrid rice system prepared by the restoring line are used for finally breeding the multi-mark dual-purpose hybrid rice with yellow leaves, purple stigma and red palea tipsIs S 7 1, temporarily named "yellow 1S".
The comparison of the leaf color, the color of the lemma tip, the column head color, the column head exposure rate, the seed production yield and the ornamental performance of each variety is shown in the table 1.
TABLE 1 comparison of index traits of Zheda 01S target improved multi-marker dual-purpose hybrid rice sterile line' yellow marker 1S
The measurement method of each index in table 1 is as follows: leaf color, stigma color, lemma tip color and other agronomic characteristics and yield: national rice variety test observation records items, methods and standards (trial); the exposure rate of the column head: DB 22-T2389-2018; the ornamental performance is as follows: the judgment of the excellence and the inferiority is that the ornamental time (the growth period length) of the colorful leaves is taken as a main standard on the premise that the non-lodging performance is suitable for ornamental.
Through variety specificity, consistency and stability identification, the bred multi-label dual-purpose hybrid rice sterile line 'yellow mark 1S', bright yellow leaf color, purple column head, red husk point, 54.2% column head exposure rate, more than or equal to 35.8% outcrossing maturing rate and 210kg seed production yield, the prepared hybrid combination has high quality, high yield and disease resistance, the Chinese accounts for more than or equal to 620 kg/mu of the prepared hybrid combination yield, the rice quality reaches the national second grade, and the rice blast disease resistance is resisted.
Compared with the parent Zheda pink rice and Zheda Huang Caihe colored rice, the marked characters such as purple stigma, red husk tip and the like are not existed in the parent, so that the marked characters can be used as the marked characters at different growth stages for fidelity and removing false, high-quality hybrid rice seeds are produced, the safe driving and the protection navigation are produced for the safe seeds, the ornamental characters can be used, and the excellent multi-angle ornamental function is realized.
Claims (7)
1. A breeding method of a multi-marker dual-purpose hybrid rice sterile line is characterized by comprising the following steps:
(1) Crossing with Zhejiang Huang Caihe as female parent and Zhejiang pink rice as male parent to obtain F 1 Then with F 1 The generation is used as male parent, zheda 01S is used as female parent for hybridization, and the obtained filial generation is backcrossed with the female parent Zheda 01S for n times to obtain BC n F 1 Generation, BC n F 1 After generation selfing, harvesting BC n F 2 Seed generation;
(2) Planting BC n F 2 Directly seeding and raising seedling, selecting yellow plant when seedling has 4-5 leaves, transplanting to field, selecting pollen abortive, male sterile plant with colored stigma and husk tip, cutting and regenerating, selecting sterile line with fertility converted into fertility, selecting sterile line with purple stigma and red husk tip 1 ；
(3) Planting BC n F 3~5 Identifying stigma exsertion rate and flowering habit by using purple stigma as a mark, identifying fertility of sterile line by using Zhejiang 01S as a contrast, and selecting sterile line S with good flowering habit 2 ；
(4) Planting BC n Performing indoor and outdoor sterility identification for F6 generation or more, and selecting sterile line S with sterility meeting standard requirement based on NY/T215-2006 issued by Ministry 3 Testing and matching with restorer to obtain hybrid F 1 ；
(5) For the obtained sterile line S 3 Identification of F 1 Selecting and reserving sterile line S with strong hybrid vigor 4 ；
(6) For the obtained sterile line S 4 Performing seed production test, selecting and reserving sterile line S easy for seed production 5 ；
(7) For the obtained sterile line S 5 Planting the variety in combination with the promoted variety, and selecting and reserving sterile line S with good ornamental value 6 ；
(8) Construction of multiple trait marker sterile line S 6 The restoring line and the hybrid rice system prepared by the restoring line are used for finally breeding the multi-mark dual-purpose hybrid rice sterile line S with yellow leaves, purple stigma and red lemma tips 7 。
2. The method for breeding the sterile line of multi-marker dual-purpose hybrid rice as claimed in claim 1, wherein in step (1), n is not less than 3.
3. The breeding method of the sterile line of the multi-marker dual-purpose hybrid rice as claimed in claim 1, wherein in the step (3), the criterion of good flowering habit is that the stigma exsertion rate is more than or equal to 50% and the flowering time in sunny days is concentrated in 10:30 to 11.
4. The method for breeding the sterile line of the multi-marker dual-purpose hybrid rice as claimed in claim 1, wherein the index for judging the heterosis in the step (5) comprises: the specific indexes of the rice yield, the rice quality and the disease resistance require that the yield is more than or equal to 600 kg/mu, the rice quality reaches more than three-level standards issued by the ministry and the rice blast resistance is achieved.
5. The method for breeding the sterile line of the multi-marker dual-purpose hybrid rice as claimed in claim 1, wherein in step (6), the characteristics of easy seed production are that the stigma exsertion rate is more than or equal to 50%, the outcrossing maturing rate is more than or equal to 30%, and the seed reproduction and production yield is more than or equal to 180kg.
6. The method for breeding the sterile line of multi-marker dual-purpose hybrid rice as claimed in claim 1, wherein in the step (7), the promoted variety is Xiushui 134.
7. The method for breeding the sterile line of multi-marker dual-purpose hybrid rice as claimed in claim 1, wherein in step (7), the ornamental value is longer growth period, higher plant height and stronger lodging resistance compared with the parent.
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| CN102668974A (en) * | 2012-06-11 | 2012-09-19 | 广西壮族自治区农业科学院水稻研究所 | Selective breeding method of hybrid rice three-line sterile line with purple leaf marker |
| CN104054570A (en) * | 2014-06-26 | 2014-09-24 | 浙江大学 | Breeding method of hybrid rice two-line sterile line with leaves carrying white midribs |
| WO2018059121A1 (en) * | 2016-09-29 | 2018-04-05 | 武汉多倍体生物科技有限公司 | Polyploid two-line hybrid rice plant and selective breeding method therefor |
| CN111264380A (en) * | 2020-03-23 | 2020-06-12 | 浙江大学 | Breeding method of colored rice two-line hybrid rice |
| CN111264382A (en) * | 2020-03-23 | 2020-06-12 | 浙江大学 | Breeding method of two-line hybrid rice of colored rice |
| CN113940272A (en) * | 2021-11-19 | 2022-01-18 | 浙江大学 | Breeding method of hybrid rice with nutrition and ornamental functions |
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- 2022-01-19 CN CN202210058148.5A patent/CN114532216B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668974A (en) * | 2012-06-11 | 2012-09-19 | 广西壮族自治区农业科学院水稻研究所 | Selective breeding method of hybrid rice three-line sterile line with purple leaf marker |
| CN104054570A (en) * | 2014-06-26 | 2014-09-24 | 浙江大学 | Breeding method of hybrid rice two-line sterile line with leaves carrying white midribs |
| WO2018059121A1 (en) * | 2016-09-29 | 2018-04-05 | 武汉多倍体生物科技有限公司 | Polyploid two-line hybrid rice plant and selective breeding method therefor |
| CN111264380A (en) * | 2020-03-23 | 2020-06-12 | 浙江大学 | Breeding method of colored rice two-line hybrid rice |
| CN111264382A (en) * | 2020-03-23 | 2020-06-12 | 浙江大学 | Breeding method of two-line hybrid rice of colored rice |
| CN113940272A (en) * | 2021-11-19 | 2022-01-18 | 浙江大学 | Breeding method of hybrid rice with nutrition and ornamental functions |
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