CN110313397B - Water-saving high-quality winter wheat selection breeding method - Google Patents
Water-saving high-quality winter wheat selection breeding method Download PDFInfo
- Publication number
- CN110313397B CN110313397B CN201910737183.8A CN201910737183A CN110313397B CN 110313397 B CN110313397 B CN 110313397B CN 201910737183 A CN201910737183 A CN 201910737183A CN 110313397 B CN110313397 B CN 110313397B
- Authority
- CN
- China
- Prior art keywords
- wheat
- quality
- generation
- selecting
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to the technical field of wheat breeding methods, and discloses a water-saving high-quality winter wheat selection breeding method. The main technical characteristics are as follows: selecting high-quality wheat and water-saving wheat parents with glutenin 5+10 subunits and the rye-base deletion molecular marker; planting the selected parents in an area suitable for planting winter wheat, and then hybridizing the two parents to obtain an F0 generation; backcrossing the F0 generation with high-quality wheat again to obtain a backcross F0 generation, and carrying out pregermination on the backcross F0 generation; the backcrossed F0 generation wheat after pregermination is planted in an area which is suitable for spring wheat planting and has the north latitude of 40.5-42 ℃; selecting high-quality marked wheat varieties in F1, F2 and F3 generations, then identifying the water-saving performance under different moisture conditions in F4-7 generations, selecting the varieties with good water-saving performance, and finally obtaining the water-saving high-quality wheat varieties. The three generations of breeding are completed within two years, the breeding time is shortened, and the selected variety has good water-saving performance, high quality and good stability of wheat variety.
Description
Technical Field
The invention belongs to the technical field of wheat progeny selection breeding methods, and particularly relates to a winter wheat progeny selection breeding method.
Background
In the breeding process of winter wheat, a variety with required performance is obtained through parent hybridization. Because winter wheat needs vernalization and tillering characteristics, the following defects exist in the current breeding: firstly, one generation of wheat is generally adopted during breeding, the breeding period is long, and the popularization of new wheat varieties is not facilitated; secondly, after the parents adopt high-quality wheat varieties and water-saving wheat to be hybridized to obtain F0 generations when selecting the water-saving high-quality winter wheat varieties, breeding from the F1 generation to the high generation is directly carried out, and the high-quality stability of the wheat is poor; thirdly, the high quality and the water saving performance are compared in each generation of breeding, so that the test steps are complicated and the test workload is large.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a water-saving high-quality winter wheat selection breeding method which has the advantages of short breeding time, low seed selection cost, less occupied land, high efficiency, pure variety and high seed selection accuracy.
In order to solve the problems, the method for selecting, pulling and breeding the water-saving high-quality winter wheat adopts the technical scheme that:
the method comprises the following steps:
first, selecting parents
Selecting a water-saving wheat material and a high-quality wheat material with a glutelin 5+10 subunit and a rye alkali deletion molecular marker as parents;
second, planting and crossing to obtain F0 generation
Planting the selected parents in an area suitable for planting winter wheat in a normal winter, then hybridizing the two parents, and taking the harvested wheat as F0 generation;
thirdly, backcrossing the F0 generation with high-quality wheat again to obtain a backcross F0 generation
Planting F0 generation and high-quality wheat parent in a field in a normal winter wheat planting season, transferring wheat seedlings of the two types of wheat to a greenhouse at the bottom of 11 months, hybridizing the F0 generation with the high-quality wheat, detecting glutelin 5+10 subunit and rye alkali deletion molecular markers by the F0 generation wheat, backcrossing the wheat containing the related markers as female parent with the high-quality wheat parent, and harvesting the mature wheat in 3 months to obtain backcrossed F0 generation seeds;
fourthly, backcross F0 generation pregermination
Carrying out germination accelerating on the backcross F0 generation;
fifthly, planting again to obtain F1 generation
Carrying out natural vernalization in summer by utilizing cold and cool climate and then carrying out selfing propagation on the backcross F0 generation wheat after germination acceleration in an area where the northern latitude is 40.5-42 ℃ and the spring wheat is suitable for planting;
sixthly, harvesting to obtain F1 generation
Harvesting wheat after planting backcross F0 generations in about 8 months, detecting glutelin 5+10 subunits and the rye-alkali deletion molecular markers before harvesting, and taking the wheat containing the glutelin 5+10 subunits and the rye-alkali deletion molecular markers as F1 generations;
seventhly, selecting high-quality marked wheat varieties
Observing the heterosis of the combination and detecting the molecular markers of the deletion of 5+10 subunits of the glutelin and the melanopsin in the F2 generation;
(II) selecting individual plants from the F3 generation, detecting glutelin 5+10 subunit and rye alkali missing molecular markers of the selected individual plants, selecting 4-5 seeds to detect IG content, and pre-evaluating the quality of the offspring gluten;
(III) selecting single plants and selecting excellent plant lines at the same time in the F4 generation, mainly selecting single plants which are strong in tillering force, more than or equal to 40 grains per spike, more than or equal to 38g in thousand kernel weight, full in grains, good in cold resistance and good in plant type by taking balance 4399 and teacher Luan 02-1 as reference, continuously detecting glutenin 5+10 subunit and rye alkali missing molecular markers, selecting 4-5 seeds to detect IG content, pre-evaluating the quality of the gluten of offspring and detecting the gluten index of the F4 generation plant line;
(IV) selecting a single plant from F5-F7 generations, selecting excellent plant lines, selecting a single plant with strong tillering force, more than or equal to 40 grains per spike, more than or equal to 38g thousand grain weight, full grains, good cold resistance and good plant type by taking balance 4399 and teacher Luan 02-1 as reference, continuously detecting molecular markers of glutenin 5+10 subunits and rye alkali loss at the plant line level, selecting 4-5 seeds to detect IG content, pre-evaluating the quality of the gluten of the offspring and detecting the gluten index of the key plant line;
eighthly, identifying the water-saving performance under different water content conditions, and selecting the strain with good water-saving performance
(I) F5-7 generation, selecting single plant or strain, high yield and quality identification
Identification conditions: performing two treatments of spring 1 water and spring 2 water without repetition, and performing water-saving high-yield quality stress resistance identification by taking balance 4399 and teacher Luan 02-1 as a contrast; selecting drought resistance, water conservation, disease resistance, solidity, grain weight and stable yield of the material by an important point, and detecting the quality and quality stability of the material and the molecular markers of deletion of glutelin 5+10 subunit and rye-base;
and (4) high yield identification: using scale 4399 as a reference, selecting a single plant or a strain line which has strong tillering capacity, grain number per ear of more than or equal to 40 grains, thousand kernel weight of more than or equal to 38g, full seeds, good cold resistance and good plant type;
and (3) quality identification: using teacher Luan 02-1 as a reference, detecting the content of insoluble gluten IG and gluten index, selecting index IG >3mg/100mg flour and gluten index >80%, and identifying and tracking deletion molecular markers of gluten 5+10 subunit and rye alkali;
(II) yield comparison test of the new strain:
repeating for 3 times, treating with spring 0 water, spring 1 water and spring 2 water, and performing water-saving high-yield quality stress resistance identification with balance 4399 and teacher Luan 02-1 as reference; the method has the advantages that the drought resistance, water conservation, disease resistance, fructification, grain weight and stable yield of strain materials harvested in the F4-F7 generation are selected, the quality and quality stability of the materials are detected, and the glutelin 5+10 subunit and the rye alkali deletion molecular markers are detected.
The additional technical characteristics are as follows:
in the step of planting again in the fifth step to obtain F1 generation, planting the backcrossed F0 generation wheat after germination in an area which is suitable for spring wheat planting and has a north latitude of 41.7-41.9 ℃;
in the fourth step of pregermination of backcross F0 generation, the pregermination time is 24 hours.
Compared with the prior art, the wheat progeny selection breeding method provided by the invention has the following advantages: because the method comprises the following steps:
first, selecting parents
Selecting a water-saving wheat material and a high-quality wheat material with a glutelin 5+10 subunit and a rye alkali deletion molecular marker as parents;
second, planting and crossing to obtain F0 generation
Planting the selected parents in an area suitable for planting winter wheat in a normal winter, then hybridizing the two parents, and taking the harvested wheat as F0 generation;
thirdly, backcrossing the F0 generation with high-quality wheat again to obtain a backcross F0 generation
Planting F0 generation and high-quality wheat parent in a field in a normal winter wheat planting season, transferring wheat seedlings of the two types of wheat to a greenhouse at the bottom of 11 months, hybridizing the F0 generation with the high-quality wheat, detecting glutelin 5+10 subunit and rye alkali deletion molecular markers by the F0 generation wheat, backcrossing the wheat containing the related markers as female parent with the high-quality wheat parent, and harvesting the mature wheat in 3 months to obtain backcrossed F0 generation seeds;
fourthly, backcross F0 generation pregermination
Carrying out germination accelerating on the backcross F0 generation;
fifthly, planting again to obtain F1 generation
Carrying out natural vernalization in summer by utilizing cold and cool climate and then carrying out selfing propagation on the backcross F0 generation wheat after germination acceleration in an area where the northern latitude is 40.5-42 ℃ and the spring wheat is suitable for planting;
sixthly, harvesting to obtain F1 generation
Harvesting wheat after planting backcross F0 generations in about 8 months, detecting glutelin 5+10 subunits and the rye-alkali deletion molecular markers before harvesting, and taking the wheat containing the glutelin 5+10 subunits and the rye-alkali deletion molecular markers as F1 generations;
seventhly, selecting high-quality marked wheat varieties
Observing the heterosis of the combination and detecting the molecular markers of the deletion of 5+10 subunits of the glutelin and the melanopsin in the F2 generation;
(II) selecting individual plants from the F3 generation, detecting glutelin 5+10 subunit and rye alkali missing molecular markers of the selected individual plants, selecting 4-5 seeds to detect IG content, and pre-evaluating the quality of the offspring gluten;
(III) selecting single plants and selecting excellent plant lines at the same time in the F4 generation, mainly selecting single plants which are strong in tillering force, more than or equal to 40 grains per spike, more than or equal to 38g in thousand kernel weight, full in grains, good in cold resistance and good in plant type by taking balance 4399 and teacher Luan 02-1 as reference, continuously detecting glutenin 5+10 subunit and rye alkali missing molecular markers, selecting 4-5 seeds to detect IG content, pre-evaluating the quality of the gluten of offspring and detecting the gluten index of the F4 generation plant line;
(IV) selecting a single plant from F5-F7 generations, selecting excellent plant lines, selecting a single plant with strong tillering force, more than or equal to 40 grains per spike, more than or equal to 38g thousand grain weight, full grains, good cold resistance and good plant type by taking balance 4399 and teacher Luan 02-1 as reference, continuously detecting molecular markers of glutenin 5+10 subunits and rye alkali loss at the plant line level, selecting 4-5 seeds to detect IG content, pre-evaluating the quality of the gluten of the offspring and detecting the gluten index of the key plant line;
eighthly, identifying the water-saving performance under different water content conditions, and selecting the strain with good water-saving performance
(I) F5-7 generation, selecting single plant or strain, high yield and quality identification
Identification conditions: performing two treatments of spring 1 water and spring 2 water without repetition, and performing water-saving high-yield quality stress resistance identification by taking balance 4399 and teacher Luan 02-1 as a contrast; selecting drought resistance, water conservation, disease resistance, solidity, grain weight and stable yield of the material by an important point, and detecting the quality and quality stability of the material and the molecular markers of deletion of glutelin 5+10 subunit and rye-base;
and (4) high yield identification: using scale 4399 as a reference, selecting a single plant or a strain line which has strong tillering capacity, grain number per ear of more than or equal to 40 grains, thousand kernel weight of more than or equal to 38g, full seeds, good cold resistance and good plant type;
and (3) quality identification: using teacher Luan 02-1 as a reference, detecting the content of insoluble gluten IG and gluten index, selecting index IG >3mg/100mg flour and gluten index >80%, and identifying and tracking deletion molecular markers of gluten 5+10 subunit and rye alkali;
(II) yield comparison test of the new strain:
repeating for 3 times, treating with spring 0 water, spring 1 water and spring 2 water, and performing water-saving high-yield quality stress resistance identification with balance 4399 and teacher Luan 02-1 as reference; the method has the advantages that the drought resistance, water conservation, disease resistance, fructification, grain weight and stable yield of strain materials harvested in the F4-F7 generation are selected, the quality and quality stability of the materials are detected, and the glutelin 5+10 subunit and the rye alkali deletion molecular markers are detected.
The method comprises the steps of firstly hybridizing a winter wheat planting region at normal temperature to obtain F0 generations, then planting the winter wheat planting region at normal temperature, then transferring wheat seedlings into a greenhouse at the bottom of 11 months, promoting the production of the wheat seedlings in the greenhouse, hybridizing an F0 generation with high-quality wheat during the growth process to obtain a backcross F0 generation, maturing the backcross F0 generation in 3 months, accelerating germination of the harvested backcross F0 generation, planting the wheat seedlings in a region suitable for spring wheat planting between 40.5 and 42 ℃ in northern latitude, harvesting the wheat seedlings in about 8 months, normally sowing the harvested wheat seedlings in the winter wheat region to perform a breeding test, completing three-generation breeding within two years, shortening the breeding time, and hybridizing the F0 generation with the high-quality wheat again to enable the high-quality performance to be more stable; firstly, observing the advantages of the combined hybrid in the F2 generation, and detecting the molecular markers of the deletion of the glutelin 5+10 subunit and the rye-triticale; selecting individual plants in the F3 generation, detecting glutelin 5+10 subunit and rye alkali missing molecular markers of the selected individual plants, selecting 4-5 seeds to detect IG content, and pre-evaluating the quality of the offspring gluten; selecting single plants in the F4 generation, selecting excellent plant lines, selecting single plants with strong tillering force, more than or equal to 40 grains per spike, more than or equal to 38g thousand-grain weight, full grains, good cold resistance and good plant types by taking balance 4399 and master Luan 02-1 as reference, continuously detecting molecular markers of glutenin 5+10 subunits and rye-alkali deficiency, selecting 4-5 seeds to detect IG content, pre-evaluating the quality of the gluten of offspring and detecting the gluten index of the F4 generation plant line; selecting single plants in the F5-7 generation, selecting excellent plant lines, selecting single plants with strong tillering force, more than or equal to 40 grains per spike, more than or equal to 38g thousand-grain weight, full grains, good cold resistance and good plant types by taking balance 4399 and master Luan 02-1 as reference, continuously detecting glutenin 5+10 subunit and rye alkali deletion molecular markers, selecting 4-5 seeds to detect IG content, pre-evaluating the quality of the offspring gluten, and detecting the gluten index of the key plant lines; then selecting single plants or strains for high yield and quality identification in the F5-7 generation, wherein the identification conditions are as follows: performing two treatments of spring 1 water and spring 2 water without repetition, and performing water-saving high-yield quality stress resistance identification by taking balance 4399 and teacher Luan 02-1 as a contrast; selecting drought resistance, water conservation, disease resistance, solidity, grain weight and stable yield of the material by an important point, and detecting the quality and quality stability of the material and the molecular markers of deletion of glutelin 5+10 subunit and rye-base; and (4) high yield identification: using scale 4399 as a reference, selecting a single plant or a strain line which has strong tillering capacity, grain number per ear of more than or equal to 40 grains, thousand kernel weight of more than or equal to 38g, full seeds, good cold resistance and good plant type; and (3) quality identification: using teacher Luan 02-1 as a reference, detecting the content of insoluble gluten IG and gluten index, selecting index IG >3mg/100mg flour and gluten index >80%, and identifying and tracking deletion molecular markers of gluten 5+10 subunit and rye alkali; finally, carrying out a new strain yield comparison test: repeating for 3 times, treating with spring 0 water, spring 1 water and spring 2 water, and performing water-saving high-yield quality stress resistance identification with balance 4399 and teacher Luan 02-1 as reference; the method has the advantages that drought resistance, water conservation, disease resistance, fructification, grain weight and stable yield of strain line materials harvested in F4-F7 generations are selected, the quality and quality stability of the materials are detected, and glutelin 5+10 subunit and rye alkali lack molecular markers are detected, so that the selected varieties are good in water conservation performance and quality, the seed selection time is shortened, the wheat varieties are good in stability, the method realizes effective combination of conventional seed selection and molecular marker auxiliary selection, and effective innovation is realized on water conservation and high quality seed selection work.
And in the step of planting again in the fifth step to obtain the F1 generation, the backcrossed F0 generation wheat after germination is planted in an area which is suitable for spring wheat planting between 41.7 and 41.9 degrees of north latitude, and is more suitable for vernalization and tillering growth of winter wheat varieties.
In the fourth step of accelerating germination of backcross F0 generations, the accelerating germination time is 24 hours, so that the vernalization effect of the wheat seeds after accelerating germination is better.
Detailed Description
The method for water-saving high-quality winter wheat selection breeding according to the present invention will be described in further detail with reference to the following embodiments.
The method for selecting and breeding wheat progeny comprises the following steps: the method comprises the following steps:
the method comprises the following steps:
first, selecting parents
Selecting a water-saving wheat material and a high-quality wheat material with a glutelin 5+10 subunit and a rye alkali deletion molecular marker as parents;
second, planting and crossing to obtain F0 generation
Planting the selected parents in an area suitable for planting winter wheat in a normal winter, then hybridizing the two parents, and taking the harvested wheat as F0 generation;
thirdly, backcrossing the F0 generation with high-quality wheat again to obtain a backcross F0 generation
Planting F0 generation and high-quality wheat parent in a field in a normal winter wheat planting season, transferring wheat seedlings of the two types of wheat to a greenhouse at the bottom of 11 months, hybridizing the F0 generation with the high-quality wheat, detecting glutelin 5+10 subunit and rye alkali deletion molecular markers by the F0 generation wheat, backcrossing the wheat containing the related markers as female parent with the high-quality wheat parent, and harvesting the mature wheat in 3 months to obtain backcrossed F0 generation seeds;
fourthly, backcross F0 generation pregermination
Carrying out germination accelerating on the backcross F0 generation;
fifthly, planting again to obtain F1 generation
Carrying out natural vernalization in summer by utilizing cold and cool climate and then carrying out selfing propagation on the backcross F0 generation wheat after germination acceleration in an area where the northern latitude is 40.5-42 ℃ and the spring wheat is suitable for planting;
sixthly, harvesting to obtain F1 generation
Harvesting wheat after planting backcross F0 generations in about 8 months, detecting glutelin 5+10 subunits and the rye-alkali deletion molecular markers before harvesting, and taking the wheat containing the glutelin 5+10 subunits and the rye-alkali deletion molecular markers as F1 generations;
seventhly, selecting high-quality marked wheat varieties
Observing the heterosis of the combination and detecting the molecular markers of the deletion of 5+10 subunits of the glutelin and the melanopsin in the F2 generation;
(II) selecting individual plants from the F3 generation, detecting glutelin 5+10 subunit and rye alkali missing molecular markers of the selected individual plants, selecting 4-5 seeds to detect IG content, and pre-evaluating the quality of the offspring gluten;
(III) selecting single plants and selecting excellent plant lines at the same time in the F4 generation, mainly selecting single plants which are strong in tillering force, more than or equal to 40 grains per spike, more than or equal to 38g in thousand kernel weight, full in grains, good in cold resistance and good in plant type by taking balance 4399 and teacher Luan 02-1 as reference, continuously detecting glutenin 5+10 subunit and rye alkali missing molecular markers, selecting 4-5 seeds to detect IG content, pre-evaluating the quality of the gluten of offspring and detecting the gluten index of the F4 generation plant line;
(IV) selecting a single plant from F5-F7 generations, selecting excellent plant lines, selecting a single plant with strong tillering force, more than or equal to 40 grains per spike, more than or equal to 38g thousand grain weight, full grains, good cold resistance and good plant type by taking balance 4399 and teacher Luan 02-1 as reference, continuously detecting molecular markers of glutenin 5+10 subunits and rye alkali loss at the plant line level, selecting 4-5 seeds to detect IG content, pre-evaluating the quality of the gluten of the offspring and detecting the gluten index of the key plant line;
eighthly, identifying the water-saving performance under different water content conditions, and selecting the strain with good water-saving performance
(I) F5-7 generation, selecting single plant or strain, high yield and quality identification
Identification conditions: performing two treatments of spring 1 water and spring 2 water without repetition, and performing water-saving high-yield quality stress resistance identification by taking balance 4399 and teacher Luan 02-1 as a contrast; selecting drought resistance, water conservation, disease resistance, solidity, grain weight and stable yield of the material by an important point, and detecting the quality and quality stability of the material and the molecular markers of deletion of glutelin 5+10 subunit and rye-base;
and (4) high yield identification: using scale 4399 as a reference, selecting a single plant or a strain line which has strong tillering capacity, grain number per ear of more than or equal to 40 grains, thousand kernel weight of more than or equal to 38g, full seeds, good cold resistance and good plant type;
and (3) quality identification: using teacher Luan 02-1 as a reference, detecting the content of insoluble gluten IG and gluten index, selecting index IG >3mg/100mg flour and gluten index >80%, and identifying and tracking deletion molecular markers of gluten 5+10 subunit and rye alkali;
(II) yield comparison test of the new strain:
repeating for 3 times, treating with spring 0 water, spring 1 water and spring 2 water, and performing water-saving high-yield quality stress resistance identification with balance 4399 and teacher Luan 02-1 as reference; the method has the advantages that the drought resistance, water conservation, disease resistance, fructification, grain weight and stable yield of strain materials harvested in the F4-F7 generation are selected, the quality and quality stability of the materials are detected, and the glutelin 5+10 subunit and the rye alkali deletion molecular markers are detected.
The method comprises the steps of firstly hybridizing a winter wheat planting region at normal temperature to obtain F0 generations, then planting the winter wheat planting region at normal temperature, then transferring wheat seedlings into a greenhouse at the bottom of 11 months, promoting the production of the wheat seedlings in the greenhouse, hybridizing an F0 generation with high-quality wheat during the growth process to obtain a backcross F0 generation, maturing the backcross F0 generation in 3 months, accelerating germination of the harvested backcross F0 generation, planting the wheat seedlings in a region suitable for spring wheat planting between 40.5 and 42 ℃ in northern latitude, harvesting the wheat seedlings in about 8 months, normally sowing the harvested wheat seedlings in the winter wheat region to perform a breeding test, completing three-generation breeding within two years, shortening the breeding time, and hybridizing the F0 generation with the high-quality wheat again to enable the high-quality performance to be more stable; firstly, observing the advantages of the combined hybrid in the F2 generation, and detecting the molecular markers of the deletion of the glutelin 5+10 subunit and the rye-triticale; selecting individual plants in the F3 generation, detecting glutelin 5+10 subunit and rye alkali missing molecular markers of the selected individual plants, selecting 4-5 seeds to detect IG content, and pre-evaluating the quality of the offspring gluten; selecting single plants in the F4 generation, selecting excellent plant lines, selecting single plants with strong tillering force, more than or equal to 40 grains per spike, more than or equal to 38g thousand-grain weight, full grains, good cold resistance and good plant types by taking balance 4399 and master Luan 02-1 as reference, continuously detecting molecular markers of glutenin 5+10 subunits and rye-alkali deficiency, selecting 4-5 seeds to detect IG content, pre-evaluating the quality of the gluten of offspring and detecting the gluten index of the F4 generation plant line; selecting single plants in the F5-7 generation, selecting excellent plant lines, selecting single plants with strong tillering force, more than or equal to 40 grains per spike, more than or equal to 38g thousand-grain weight, full grains, good cold resistance and good plant types by taking balance 4399 and master Luan 02-1 as reference, continuously detecting glutenin 5+10 subunit and rye alkali deletion molecular markers, selecting 4-5 seeds to detect IG content, pre-evaluating the quality of the offspring gluten, and detecting the gluten index of the key plant lines; then selecting single plants or strains for high yield and quality identification in the F5-7 generation, wherein the identification conditions are as follows: performing two treatments of spring 1 water and spring 2 water without repetition, and performing water-saving high-yield quality stress resistance identification by taking balance 4399 and teacher Luan 02-1 as a contrast; selecting drought resistance, water conservation, disease resistance, solidity, grain weight and stable yield of the material by an important point, and detecting the quality and quality stability of the material and the molecular markers of deletion of glutelin 5+10 subunit and rye-base; and (4) high yield identification: using scale 4399 as a reference, selecting a single plant or a strain line which has strong tillering capacity, grain number per ear of more than or equal to 40 grains, thousand kernel weight of more than or equal to 38g, full seeds, good cold resistance and good plant type; and (3) quality identification: using teacher Luan 02-1 as a reference, detecting the content of insoluble gluten IG and gluten index, selecting index IG >3mg/100mg flour and gluten index >80%, and identifying and tracking deletion molecular markers of gluten 5+10 subunit and rye alkali; finally, carrying out a new strain yield comparison test: repeating for 3 times, treating with spring 0 water, spring 1 water and spring 2 water, and performing water-saving high-yield quality stress resistance identification with balance 4399 and teacher Luan 02-1 as reference; the method has the advantages that drought resistance, water conservation, disease resistance, fructification, grain weight and stable yield of strain line materials harvested in F4-F7 generations are selected, the quality and quality stability of the materials are detected, and glutelin 5+10 subunit and rye alkali lack molecular markers are detected, so that the selected varieties are good in water conservation performance and quality, the seed selection time is shortened, the wheat varieties are good in stability, the method realizes effective combination of conventional seed selection and molecular marker auxiliary selection, and effective innovation is realized on water conservation and high quality seed selection work.
And in the step of planting again in the fifth step to obtain F1 generations, planting the backcrossed F0 generation wheat after germination acceleration in an area which is suitable for spring wheat planting between 41.7 and 41.9 degrees north latitude, and is more suitable for vernalization and tillering growth of winter wheat varieties.
In the fourth step of accelerating germination of backcross F0 generations, the accelerating germination time is 24 hours, so that the vernalization effect of the wheat seeds after accelerating germination is better.
The scope of the present invention is not limited to the above-mentioned examples, and is within the scope of the present invention as long as the structure of the method for selecting and breeding wheat progeny is the same as or similar to that of the method for selecting and breeding wheat progeny of the present invention.
Claims (3)
1. The method for water-saving high-quality winter wheat selection breeding is characterized by comprising the following steps:
the method comprises the following steps:
first, selecting parents
Selecting a water-saving wheat material and a high-quality wheat material with a glutelin 5+10 subunit and a rye alkali deletion molecular marker as parents;
second, planting and crossing to obtain F0 generation
Planting the selected parents in an area suitable for planting winter wheat in a normal winter, then hybridizing the two parents, and taking the harvested wheat as F0 generation;
thirdly, backcrossing the F0 generation with high-quality wheat again to obtain a backcross F0 generation
Planting F0 generation and high-quality wheat parent in a field in a normal winter wheat planting season, transferring wheat seedlings of the two types of wheat to a greenhouse at the bottom of 11 months, hybridizing the F0 generation with the high-quality wheat, detecting glutelin 5+10 subunit and rye alkali deletion molecular markers by the F0 generation wheat, backcrossing the wheat containing the related markers as female parent with the high-quality wheat parent, and harvesting the mature wheat in 3 months to obtain backcrossed F0 generation seeds;
fourthly, backcross F0 generation pregermination
Carrying out germination accelerating on the backcross F0 generation;
fifthly, planting again to obtain F1 generation
Carrying out natural vernalization in summer by utilizing cold and cool climate and then carrying out selfing propagation on the backcross F0 generation wheat after germination acceleration in an area where the northern latitude is 40.5-42 ℃ and the spring wheat is suitable for planting;
sixthly, harvesting to obtain F1 generation
Harvesting wheat after planting backcross F0 generations in about 8 months, detecting glutelin 5+10 subunits and the rye-alkali deletion molecular markers before harvesting, and taking the wheat containing the glutelin 5+10 subunits and the rye-alkali deletion molecular markers as F1 generations;
seventhly, selecting high-quality marked wheat varieties
Observing the heterosis of the combination and detecting the molecular markers of the deletion of 5+10 subunits of the glutelin and the melanopsin in the F2 generation;
(II) selecting individual plants from the F3 generation, detecting glutelin 5+10 subunit and rye alkali missing molecular markers of the selected individual plants, selecting 4-5 seeds to detect IG content, and pre-evaluating the quality of the offspring gluten;
(III) selecting single plants and selecting excellent plant lines at the same time in the F4 generation, mainly selecting single plants which are strong in tillering force, more than or equal to 40 grains per spike, more than or equal to 38g in thousand kernel weight, full in grains, good in cold resistance and good in plant type by taking balance 4399 and teacher Luan 02-1 as reference, continuously detecting glutenin 5+10 subunit and rye alkali missing molecular markers, selecting 4-5 seeds to detect IG content, pre-evaluating the quality of the gluten of offspring and detecting the gluten index of the F4 generation plant line;
(IV) selecting a single plant from F5-F7 generations, selecting excellent plant lines, selecting a single plant with strong tillering force, more than or equal to 40 grains per spike, more than or equal to 38g thousand grain weight, full grains, good cold resistance and good plant type by taking balance 4399 and teacher Luan 02-1 as reference, continuously detecting molecular markers of glutenin 5+10 subunits and rye alkali loss at the plant line level, selecting 4-5 seeds to detect IG content, pre-evaluating the quality of the gluten of the offspring and detecting the gluten index of the key plant line;
eighthly, identifying the water-saving performance under different water content conditions, and selecting the strain with good water-saving performance
(I) F5-F7 generations, selecting single plant or strain, and making high yield and quality identification
Identification conditions: performing two treatments of spring 1 water and spring 2 water without repetition, and performing water-saving high-yield quality stress resistance identification by taking balance 4399 and teacher Luan 02-1 as a contrast; selecting drought resistance, water conservation, disease resistance, solidity, grain weight and stable yield of the material by an important point, and detecting the quality and quality stability of the material and the molecular markers of deletion of glutelin 5+10 subunit and rye-base;
and (4) high yield identification: using scale 4399 as a reference, selecting a single plant or a strain line which has strong tillering capacity, grain number per ear of more than or equal to 40 grains, thousand kernel weight of more than or equal to 38g, full seeds, good cold resistance and good plant type;
and (3) quality identification: using teacher Luan 02-1 as a reference, detecting the content of insoluble gluten IG and gluten index, selecting index IG >3mg/100mg flour and gluten index >80%, and identifying and tracking deletion molecular markers of gluten 5+10 subunit and rye alkali;
(II) yield comparison test of the new strain:
repeating for 3 times, treating with spring 0 water, spring 1 water and spring 2 water, and performing water-saving high-yield quality stress resistance identification with balance 4399 and teacher Luan 02-1 as reference; the method has the advantages that the drought resistance, water conservation, disease resistance, fructification, grain weight and stable yield of strain materials harvested in the F4-F7 generation are selected, the quality and quality stability of the materials are detected, and the glutelin 5+10 subunit and the rye alkali deletion molecular markers are detected.
2. The method for water-saving high-quality winter wheat selection breeding according to claim 1, characterized in that: and in the step of planting again in the fifth step to obtain F1 generations, planting the backcrossed F0 generation wheat after germination in an area which is suitable for spring wheat planting and has a north latitude of 41.7-41.9 degrees.
3. The method for water-saving high-quality winter wheat selection breeding according to claim 1, characterized in that: in the fourth step of pregermination of backcross F0 generation, the pregermination time is 24 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910737183.8A CN110313397B (en) | 2019-08-11 | 2019-08-11 | Water-saving high-quality winter wheat selection breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910737183.8A CN110313397B (en) | 2019-08-11 | 2019-08-11 | Water-saving high-quality winter wheat selection breeding method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110313397A CN110313397A (en) | 2019-10-11 |
| CN110313397B true CN110313397B (en) | 2022-02-18 |
Family
ID=68125904
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910737183.8A Active CN110313397B (en) | 2019-08-11 | 2019-08-11 | Water-saving high-quality winter wheat selection breeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110313397B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111165341B (en) * | 2020-01-16 | 2022-03-18 | 河北省农林科学院旱作农业研究所 | Water-saving index breeding method of water-saving wheat |
| CN114680039A (en) * | 2022-04-18 | 2022-07-01 | 山东省农业科学院作物研究所 | Breeding method of winter wheat molecular marker assisted selection generation-adding technology |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102197784A (en) * | 2011-02-11 | 2011-09-28 | 河北省农林科学院旱作农业研究所 | Directed cultivation method for drought-resisting water-saving variety of winter wheat |
| CN106993527A (en) * | 2017-03-29 | 2017-08-01 | 河北省农林科学院旱作农业研究所 | Good quality and high output Winter Wheat Variety Breeding method |
| CN107333643A (en) * | 2017-07-21 | 2017-11-10 | 河北省农林科学院旱作农业研究所 | A kind of resisting drought saving water strong gluten wheat directed cultivation method |
| CN110089421A (en) * | 2019-05-29 | 2019-08-06 | 河北省农林科学院旱作农业研究所 | The method of wheat hybridizing offspring choice breeding |
-
2019
- 2019-08-11 CN CN201910737183.8A patent/CN110313397B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102197784A (en) * | 2011-02-11 | 2011-09-28 | 河北省农林科学院旱作农业研究所 | Directed cultivation method for drought-resisting water-saving variety of winter wheat |
| CN106993527A (en) * | 2017-03-29 | 2017-08-01 | 河北省农林科学院旱作农业研究所 | Good quality and high output Winter Wheat Variety Breeding method |
| CN107333643A (en) * | 2017-07-21 | 2017-11-10 | 河北省农林科学院旱作农业研究所 | A kind of resisting drought saving water strong gluten wheat directed cultivation method |
| CN110089421A (en) * | 2019-05-29 | 2019-08-06 | 河北省农林科学院旱作农业研究所 | The method of wheat hybridizing offspring choice breeding |
Non-Patent Citations (1)
| Title |
|---|
| 抗旱丰产型冬小麦新品种选育方法的探讨;栗雨勤等;《中国农学通报》;19971230(第06期);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110313397A (en) | 2019-10-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103262788A (en) | Rapid breeding method for salt-tolerance rice variety | |
| JP2018533961A (en) | Polyploid 2 hybrid rice and its breeding method | |
| CN111011131B (en) | Breeding method combining rapid development technology, molecular marker technology and conventional breeding of wheat | |
| CN108377899B (en) | Breeding method for auxiliary selection of corn hybridization induced haploid by yellow-green seedling marker | |
| CN110313397B (en) | Water-saving high-quality winter wheat selection breeding method | |
| CN103609428A (en) | Method for cultivating corn allopolyploid by using unreduced gamete characteristic of tripsacum dactyloides | |
| CN101904297A (en) | Rice osms4 mutant-based seed production, seed reproduction and two-line hybrid breeding methods | |
| CN113796306B (en) | Breeding method for cultivating Huang-Huai-japonica rice variety by utilizing cold region rice germplasm resources | |
| CN110972931A (en) | Breeding method of disease-resistant bolting-resistant spring Chinese cabbage | |
| CN101743902A (en) | Construction of Brassica napus recessive nuclear sterile near-isogenic line and single cross hybrid production technology | |
| CN1264400C (en) | Method for breeding super hybridized rice | |
| CN110679478A (en) | Breeding method of forage grass type BMR sorghum sterile line | |
| US20200283785A1 (en) | Method for improving rice yield by jointly knocking out aba receptor pyl family genes and use thereof | |
| CN114009334A (en) | Breeding method of starter propagation wheat | |
| CN101176425B (en) | Method for selecting and cultivating hybridized rice infertility series resisting ear germination using molecule marker | |
| CN111280052A (en) | Breeding method of corn hybrid | |
| CN102100175A (en) | Breeding and hybrid seed-producing method of onion male sterile line | |
| CN101218892A (en) | Hybridization rice parent strain trilinear pure-preservation and reproducing method | |
| CN107251833B (en) | A kind of breeding method improveing cotton prematurity | |
| CN113079984B (en) | Production method of rice two-line photo-thermo-sensitive male nuclear sterile line stock | |
| CN115152625A (en) | Breeding method of early-maturing summer maize inbred line | |
| CN111837940B (en) | Method for producing seeds of corn plant holomorphic single cross hybrid | |
| CN114680039A (en) | Breeding method of winter wheat molecular marker assisted selection generation-adding technology | |
| CN113273460A (en) | Lodging-resistant conventional japonica rice breeding method | |
| CN107251834A (en) | A kind of selection of precocious intermediate keng rice kind |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |