CN101218892A - Hybridization rice parent strain trilinear pure-preservation and reproducing method - Google Patents
Hybridization rice parent strain trilinear pure-preservation and reproducing method Download PDFInfo
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- CN101218892A CN101218892A CNA2008100452802A CN200810045280A CN101218892A CN 101218892 A CN101218892 A CN 101218892A CN A2008100452802 A CNA2008100452802 A CN A2008100452802A CN 200810045280 A CN200810045280 A CN 200810045280A CN 101218892 A CN101218892 A CN 101218892A
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- 238000000034 method Methods 0.000 title claims abstract description 31
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 28
- 235000009566 rice Nutrition 0.000 title claims abstract description 28
- 238000009396 hybridization Methods 0.000 title claims description 10
- 238000004321 preservation Methods 0.000 title claims description 10
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000196324 Embryophyta Species 0.000 claims abstract description 90
- 238000012423 maintenance Methods 0.000 claims abstract description 64
- 241000209094 Oryza Species 0.000 claims abstract description 27
- 238000009395 breeding Methods 0.000 claims description 51
- 230000001488 breeding effect Effects 0.000 claims description 50
- 239000000203 mixture Substances 0.000 claims description 12
- 238000011084 recovery Methods 0.000 claims description 12
- 235000013339 cereals Nutrition 0.000 claims description 8
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 4
- 240000005373 Panax quinquefolius Species 0.000 claims description 4
- 230000035558 fertility Effects 0.000 claims description 4
- 230000010152 pollination Effects 0.000 claims description 4
- 230000005070 ripening Effects 0.000 claims description 4
- 230000035800 maturation Effects 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 2
- 230000001902 propagating effect Effects 0.000 abstract 6
- 238000000746 purification Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000001953 sensory effect Effects 0.000 description 3
- 230000000007 visual effect Effects 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000009400 out breeding Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 230000005226 mechanical processes and functions Effects 0.000 description 1
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Abstract
The invention discloses a three-line purifying propagating method for a hybrid rice parent. The purifying propagating method of the invention includes three steps of single plant selection, strain purifying and stock seed propagating. The invention persists in giving priority to purifying and uses one part of the seeds obtained after single plant selection and strain purifying on the strain purifying of a maintenance line of the next season and the other part on the purifying propagating on the sterile line, and taking the maintenance line as the core, maintains the stability of the sterile line through continuous backcross. The purifying propagating of the recover line only needs a single plant section and a strain purifying. The invention has the advantages of simple, convenient and easy operation as well as fast propagating and can propagate and produce the three-line seeds for the commercial hybrid rice parent according with the national standard only by two growing seasons, thus not only ensuring the stability of colony, avoiding the losing of beneficial genes, but also meeting the three-line hybrid-resistant purifying requirements for the hybrid rice parent.
Description
Technical field
The present invention relates to the rice paddy seed production technical field, relate in particular to a kind of hybridization rice parent strain trilinear pure-preservation and reproducing method.
Background technology
China has formed large-scale production at present and breeding hybrid rice parent three is, comprise male sterile line, maintenance line and recovery system, because in the breeding and production of hybrid seeds process that hybrid rice parent three is, infiltrate maintenance line in the male sterile line easily, recover system and other conventional varieties or hybrid rice, cause mechanical admixture and biology hybrid, so the maintenance of its seed purity is very difficult.Existing hybrid rice parent three protects pure method to pay attention to male sterile line, based on purification, mainly containing supporting method of purification and three is seven garden methods, supporting method of purification is with paired test cross, the offspring is accredited as core, comprise the roguing pairing backcrossing, test cross, choosing is tied to form backcrossing, test cross, F1 identifies, selects three series mating surely according to the F1 qualification result, and forming three is breeder's stock, program and the operating procedure with a series of complexity such as breeding of planting produced and produced to original seed, method of operating complexity not only, technical difficulty is big, and length consuming time, backcrosses from roguing, test cross begins, use parent's three line seed to obtaining to produce, will plant 5 at least more than the season of growth; Three is that seven garden methods are identified with strain system and typicalness is chosen as the basis, than supporting method of purification slightly more simply, but still will to set up 7 of plant garden, strain nurseries etc. be that can to finish three be the production of breeder's stock in the garden, and its cycle is longer, need could obtain production parent's three line seed more than 6 seasons of growth.In a word, existing hybrid rice parent three is that the pure method complicated operation of guarantor, technical difficulty are big, and the cycle is long, cost is high, does not meet the requirement of efficient low-consume modern agriculture, and be difficult to be undertaken by rules in actual mechanical process, usually causing parent three is that purity can't guarantee.
Summary of the invention
The objective of the invention is to overcome above shortcomings in the prior art, provide a kind of method of operating parent three simple, with short production cycle, that can breed and produce the commercialization hybrid rice parent three line seed that meets country's kind substandard to protect the pure breeding culturing method.
To achieve these goals, the invention provides following technical scheme:
A kind of hybridization rice parent strain trilinear pure-preservation and reproducing method is characterized in that this method may further comprise the steps:
(1) individual plant is selected: maintenance line, the individual plant that recovers to be are selected independently to carry out respectively:
1. be that individual plant is planted respectively in fertility is even, the field face is smooth, field piece is easily arranged in filling with maintainer line of hybrid rice, recovery;
2. primary election: selecting to have former kind typicalness maintenance line individual plant shape, neat and consistent during heading respectively, recovering is each mark of listing respectively more than 1000 strains of individual plant, be preferably the heading Sheng phase period of primary election, the rice strain heading of promptly full field reaches for 50～80% period; Selected individual plant is preferably the individual plant of main fringe heading on the same day;
3. final election: when ripe with the maintenance line individual plant that 2. primary election obtained in the step, to recover be that individual plant (individual plant of the mark of listing when earing) carries out final election respectively, eliminate in maturing stage (as proterties such as seed color, seed shape, plant heights) and the inconsistent individual plant of former kind typicalness shape in appearance;
4. indoor selection: with the maintenance line individual plant that 3. final election obtained in the step, to recover be to carry out indoor selection respectively after the individual plant results, acquisition meets the individual plant of former kind feature, proterties such as indoor selection is mainly wide with spike length, ripening rate, thousand kernel weight, grain length, grain, seed aspect ratio are accepted or rejected, all quantitative characters are all based on mean value, and its standard should be controlled in positive and negative 5%;
5. the seed with individual plant plant that indoor selection obtains keeps, and quantum count that each individual plant is reserved seed for planting is generally 50～100, and the seed that recovers system is used for to be single-strain planting, promptly to be used for strain system and to protect the plantation of pure garden by strain; A part of seed of maintenance line is used for strain system and protects the plantation of pure garden, is used for the breeding of male sterile line after all the other seeds mix, and promptly is used for the plantation of breeding stock garden;
(2) strain system protects pure:
1. the maintenance line that the indoor selection of individual plant is obtained, recovery are the part seed of plant, are single-strain planting by strain;
2. ear to the maturing stage, selecting to have former kind typicalness maintenance line strain shape, neat and consistent system respectively, recover with visual sensory is that strain is the mark of listing, the quantity that strain system is selected is decided according to producing with planting, at least keep more than 200 strain systems, in middle roguing is, each strain system selects a small amount of individual plant to protect pure garden as the strain system in next season, general 5～10 individual plants of each strain mooring, with roguing in the maintenance line is after the seed of all the other individual plants mixes, the maintenance line of breeding the garden as next step so circulates with planting;
(3) breeding stock:
The maintenance line seed mixture that the indoor selection of individual plant is obtained, or strain system is protected in the pure garden, the seed mixture of roguing system in the maintenance line, be used for the breeding of male sterile line, this is the breeding stock garden, male sterile line and maintenance line compare single-strain planting by the row of 8: 2 or 10: 2 in the original seed propagation nursery, other field management is undertaken by the measure of general male sterile line, used maintenance line only is used for providing pollen to male sterile line in the breeding stock garden, in after its pollination end 7 days, the maintenance line flush with ground is sliced off, and used maintenance line will be protected pure garden from second step strain system again in following season; The used male sterile line in breeding stock garden should be removed assorted strain in the field growing stage as far as possible.
Described strain system protects and purely can carry out simultaneously with breeding stock, and the guarantor's pure breeding that recovers system is grown and only carried out that individual plant is selected and the pure step of the guarantor of strain system.
Each season of growth is selected first step individual plant to be placed in the second strain system that goes on foot in the strain system pure garden of guarantor and carries out simultaneously from now on, has only strain system pure garden of guarantor and breeding stock garden, protects the pure breeding culturing method so the present invention is called two garden systems.
Compared with prior art, the invention has the beneficial effects as follows: the present invention changes the past based on purification, heavy male sterile line and the viewpoint of light maintenance line, adhere to protect pure, with maintenance line through individual plant select, that the guarantor of strain system that a seed part that pure back obtains is used for next maintenance line in season protects in strain system is pure, a part is used for guarantor's pure breeding of male sterile line and grows, to keep is core, keeping the stability of male sterile line, the guarantor's pure breeding that recovers system is grown and only need be carried out that individual plant is selected and strain is that the guarantor is pure by continuous backcross (constantly repeating for the 3rd step).The present invention is simple and easy to do, reproduction speed is fast, only need two seasons of growth can breed and produce and meet the commercialization hybrid rice parent three line seed that substandard is planted by country, both guaranteed the stability of colony, avoided losing of beneficial gene, what meet hybrid rice parent three again and be anti-assortedly protects pure requirement.
Description of drawings
Fig. 1 is a three series mating method of purification operating process schematic diagram.
Fig. 2 is three to be seven garden method operating process schematic diagrames.
Fig. 3 is an operating process schematic diagram of the present invention.
Fig. 4 is a field planting schematic diagram of the present invention.
Identify among the figure: 1-maintenance line/recovery is single-strain planting district (protecting pure garden), 2-strain nursery, 3-breeding stock garden (sterile line propagation district), 4-male sterile line, 5-maintenance line.
Embodiment
The present invention is described in further detail below in conjunction with specific embodiment.
A kind of hybridization rice parent strain trilinear pure-preservation and reproducing method may further comprise the steps:
(1) individual plant is selected:
1. maintainer line of hybrid rice C125B, recovery being tied to form extensive 983 individual plants plants respectively in fertility is even, the field face is smooth, proving ground is easily arranged in filling;
2. primary election: heading the Sheng phase select respectively to have the heading on the same day of former kind typicalness main fringe shape, neat and consistent 1000 strains of maintenance line C125B individual plant, recover to be tied to form the extensive 983 individual plants 1000 strains mark of listing respectively;
3. final election: when ripe with maintenance line C125B individual plant that primary election obtained, recover to be tied to form extensive 983 individual plants and carry out final election respectively, eliminate in the maturing stage and (comprise proterties such as seed color, seed shape, plant height) in appearance and the inconsistent individual plant of former kind typicalness shape;
4. indoor selection: carry out indoor selection respectively with final election gained maintenance line C125B individual plant, after recovering to be tied to form extensive 983 individual plants results, acquisition meets the individual plant of former kind feature, proterties such as indoor selection is mainly wide with spike length, ripening rate, thousand kernel weight, grain length, grain, seed aspect ratio are accepted or rejected, all quantitative characters are all based on mean value, and its standard is controlled in positive and negative 5%;
5. the seed of individual plant plant that indoor selection obtains is kept, quantum count that each individual plant is reserved seed for planting is 50～100, and recovering to be tied to form extensive 983 seed, to be used for by strain be single-strain planting, promptly is used for strain system and protects pure garden and plant; Maintenance line? number a part of seed be used for strain system and protect the plantation of pure garden, all the other seeds mix, and are used for the breeding of male sterile line C125A, this is the breeding stock garden;
(2) strain system protects pure:
1. the maintenance line C125B plant that the indoor selection of individual plant is obtained, recovery are tied to form the part seed of extensive 983 plant, are single-strain planting by strain respectively;
2. ear to the maturing stage, to select to have former kind typicalness maintenance line C125B shape, neat and consistent strain be 200, to recover to be tied to form extensive 983 strains be 200 marks of listing with visual sensory, in middle roguing is, select 5～10 individual plants in each strain system, protect pure garden with planting as the strain system in next season, with roguing in the maintenance line is after the seed of all the other individual plants mixes, as next step breeding garden with kind;
(3) breeding stock
The seed mixture that the indoor selection of maintenance line C125B individual plant is obtained, or strain system is protected in the pure garden, the seed mixture that middle roguing is, be used for the breeding of male sterile line C125A, this is the breeding stock garden, male sterile line C125A and maintenance line C125B compare single-strain planting by 8: 2 row in the propagation nursery, other field management is undertaken by the measure of general male sterile line, used maintenance line only is used for providing pollen to male sterile line in the breeding stock garden, in after its pollination end 7 days, the maintenance line flush with ground is sliced off, and used maintenance line will be protected pure garden from second step strain system again in following season; The used male sterile line in breeding stock garden is removed assorted strain in the field growing stage as far as possible;
The described strain guarantor of system is pure to carry out simultaneously with breeding stock.
The male sterile line C125A seed that the recovery that pure garden results are protected by strain system is tied to form grey 983 seeds, breeding stock garden results has carried out breeding stock in the proving ground, the seed of breeding is checked through the Sichuan seed quality monitoring testing station, the result is, male sterile line C125A identifies several 1200 strains of strain, find 1 assorted strain, the varietal purity of male sterile line C125A is 99.9%, reaches national original seed standard (this standard is 〉=99.9%); Recovery is tied to form extensive 983 and identifies several 1100 strains of strain, finds 1 assorted strain, and recovering to be tied to form extensive 983 varietal purities is 99.9%, reaches national original seed standard (this standard is 〉=99.9%).
A kind of hybridization rice parent strain trilinear pure-preservation and reproducing method may further comprise the steps:
(1) individual plant is selected:
1. with maintainer line of hybrid rice C150B single-strain planting in fertility evenly, the field face is smooth, irritate and arrange proving ground easily;
2. primary election: maintenance line C150B individual plant 1200 strains of selecting to have the heading on the same day of former kind typicalness main fringe shape, neat and consistent in the heading Sheng phase mark of listing;
3. final election: when ripe the maintenance line C150B individual plant that primary election obtained is carried out final election, eliminate in the maturing stage and (comprise proterties such as seed color, seed shape, plant height) in appearance and the inconsistent individual plant of former kind typicalness shape;
4. indoor selection: will carry out indoor selection after the final election gained maintenance line C150B individual plant results, acquisition meets the individual plant of former kind feature, proterties such as indoor selection is mainly wide with spike length, ripening rate, thousand kernel weight, grain length, grain, seed aspect ratio are accepted or rejected, all quantitative characters are all based on mean value, and its standard is controlled in positive and negative 5%;
5. the seed with individual plant plant that indoor selection obtains keeps, and quantum count that each individual plant is reserved seed for planting is 50～100, and a part of seed is used for strain system and protects the plantation of pure garden, and all the other seeds mix, and are used for the breeding of male sterile line C150A, and this is the breeding stock garden;
(2) strain system protects pure:
1. the part seed of the maintenance line C150B plant that the indoor selection of individual plant is obtained is a single-strain planting by strain;
2. ear to the maturing stage, selecting to have strain shape, neat and consistent of former kind typicalness with visual sensory is 250 marks of listing, in middle roguing is, select 5～10 individual plants in each strain system, strain nursery as next season is protected pure garden, with middle roguing is after the seed of all the other individual plants mixes, as next step breeding garden with kind;
(3) breeding stock
The seed mixture that the indoor selection of maintenance line C150B individual plant is obtained, or strain system is protected in the pure garden, the seed mixture that middle roguing is, be used for the breeding of male sterile line C150A, this is the breeding stock garden, male sterile line C150A and maintenance line C150B compare single-strain planting by 10: 2 row in the propagation nursery, other field management is undertaken by the measure of general male sterile line, used maintenance line only is used for providing pollen to male sterile line in the breeding stock garden, in after its pollination end 7 days, the maintenance line flush with ground is sliced off, and used maintenance line will be protected pure garden from second step strain system again in following season; The used male sterile line in breeding stock garden is removed assorted strain in the field growing stage as far as possible;
The described strain guarantor of system is pure to carry out simultaneously with breeding stock.
The male sterile line C150A seed that said method is obtained has carried out breeding stock in the proving ground, the seed of breeding is through the check of Sichuan seed quality monitoring testing station, and the result is that male sterile line C150A identifies several 1600 strains of strain, finds 1 assorted strain; The varietal purity of male sterile line C150A is 99.94%, reaches national original seed standard.
Claims (4)
1. hybridization rice parent strain trilinear pure-preservation and reproducing method is characterized in that this method may further comprise the steps:
(1) individual plant is selected: maintenance line, the individual plant that recovers to be are selected independently to carry out respectively:
1. be that individual plant is planted respectively in fertility is even, the field face is smooth, field piece is easily arranged in filling with maintainer line of hybrid rice, recovery;
2. primary election: selecting during heading to have former kind typicalness maintenance line individual plant shape, neat and consistent, recover is each mark of listing respectively more than 1000 strains of individual plant;
3. final election: with maintenance line individual plant that primary election obtained, to recover be that individual plant carries out final election respectively when maturation, eliminate the maturing stage in appearance with the inconsistent individual plant of former kind typicalness shape;
4. indoor selection: with maintenance line individual plant, recovery that final election obtained is to carry out indoor selection respectively after individual plant is gathered in the crops, acquisition meets the individual plant of former kind feature, proterties such as indoor selection is mainly wide with spike length, ripening rate, thousand kernel weight, grain length, grain, seed aspect ratio are accepted or rejected, all quantitative characters are all based on mean value, and its standard should be controlled in positive and negative 5%;
5. the seed with individual plant plant that indoor selection obtains keeps, and quantum count that each individual plant is reserved seed for planting is 50～100, and the seed that recovers system is used for to be single-strain planting, promptly to be used for strain system and to protect the plantation of pure garden by strain; A part of seed of maintenance line is used for strain system and protects the plantation of pure garden, is used for the breeding of male sterile line after all the other seeds mix, and promptly is used for the plantation of breeding stock garden;
(2) strain system protects pure:
1. the maintenance line that the indoor selection of individual plant is obtained, recovery are the part seed of plant, are single-strain planting by strain;
2. ear to the maturing stage, selecting to have former kind typicalness maintenance line strain shape, neat and consistent system respectively, recovering is that strain is the mark of listing, the quantity that selected maintenance line strain is, recovery is strain system is respectively more than 200, in middle roguing is, each strain system selects 5～10 individual plants to protect pure garden as the strain system in next season, with roguing in the maintenance line is after the seed of all the other individual plants mixes, as the maintenance line in next step breeding garden with kind, so circulation;
(3) breeding stock:
The maintenance line seed mixture that the indoor selection of individual plant is obtained, or strain system is protected in the pure garden, the seed mixture of roguing system in the maintenance line, be used for the breeding of male sterile line, this is the breeding stock garden, male sterile line and maintenance line compare single-strain planting by the row of 8: 2 or 10: 2 in the original seed propagation nursery, other field management is undertaken by the measure of general male sterile line, used maintenance line only is used for providing pollen to male sterile line in the breeding stock garden, in after its pollination end 7 days, the maintenance line flush with ground is sliced off, and used maintenance line will be protected pure garden from second step strain system again in following season; The used male sterile line in breeding stock garden should be removed assorted strain in the field growing stage as far as possible.
2. hybridization rice parent strain trilinear pure-preservation and reproducing method according to claim 1 is characterized in that it is the heading Sheng phase that described individual plant is selected the period of primary election in the step, and the rice strain heading of promptly full field reaches for 50～80% period.
3. hybridization rice parent strain trilinear pure-preservation and reproducing method according to claim 1 is characterized in that it is the individual plant of main fringe heading on the same day that described individual plant is selected the individual plant of primary election in the step.
4. garden of realizing hybridization rice parent strain trilinear pure-preservation and reproducing is characterized in that pure garden is protected by strain system in this garden and the breeding stock garden is formed, and described strain system protects that individual plant that pure garden is used for maintenance line or recovers system is selected and the guarantor of strain system is pure, and plant is a single-strain planting by strain in the garden; Described breeding stock garden is used for guarantor's pure breeding of male sterile line grows, and male sterile line and maintenance line compare single-strain planting by the row of 8: 2 or 10: 2 in the garden.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640701A (en) * | 2012-05-16 | 2012-08-22 | 中国水稻研究所 | Selecting and breeding method for long-grained hybrid japonica rice |
| CN103380727A (en) * | 2013-07-25 | 2013-11-06 | 巢湖春友种业科技有限公司 | Hybrid rice seed production three-line support method |
| CN107396827A (en) * | 2016-05-19 | 2017-11-28 | 上海天谷生物科技股份有限公司 | A kind of saving water, resisting drought rice CMS line purifies propagation method |
| CN107810858A (en) * | 2017-11-20 | 2018-03-20 | 江苏沿海地区农业科学研究所 | One kind views and admires sunflower stock breeding method |
| CN109496726A (en) * | 2018-10-17 | 2019-03-22 | 安徽新世纪农业有限公司 | A kind of three garden field formula wheat breed preferred method |
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2008
- 2008-01-29 CN CN2008100452802A patent/CN101218892B/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640701A (en) * | 2012-05-16 | 2012-08-22 | 中国水稻研究所 | Selecting and breeding method for long-grained hybrid japonica rice |
| CN103380727A (en) * | 2013-07-25 | 2013-11-06 | 巢湖春友种业科技有限公司 | Hybrid rice seed production three-line support method |
| CN107396827A (en) * | 2016-05-19 | 2017-11-28 | 上海天谷生物科技股份有限公司 | A kind of saving water, resisting drought rice CMS line purifies propagation method |
| CN107810858A (en) * | 2017-11-20 | 2018-03-20 | 江苏沿海地区农业科学研究所 | One kind views and admires sunflower stock breeding method |
| CN107810858B (en) * | 2017-11-20 | 2021-04-02 | 江苏沿海地区农业科学研究所 | Ornamental sunflower fine variety breeding method |
| CN109496726A (en) * | 2018-10-17 | 2019-03-22 | 安徽新世纪农业有限公司 | A kind of three garden field formula wheat breed preferred method |
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| CN101218892B (en) | 2010-07-14 |
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Address after: 611500 Renshou village, Yicheng Town, Chengdu, Sichuan, Qionglai Patentee after: Chengdu Jinzhuo Agricultural Co., Ltd. Address before: 611500 Renshou village, Yicheng Town, Chengdu, Sichuan, Qionglai Patentee before: Chengdu Jinzhuo Agricultural Co., Ltd. |
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Denomination of invention: Hybridization rice parent strain trilinear pure-preservation and reproducing method Effective date of registration: 20141022 Granted publication date: 20100714 Pledgee: Chengdu Productivity Promotion Center Pledgor: Chengdu Jinzhuo Agricultural Co., Ltd. Registration number: 2014990000880 |
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Effective date of registration: 20201019 Granted publication date: 20100714 |