CN103380727A - Hybrid rice seed production three-line support method - Google Patents
Hybrid rice seed production three-line support method Download PDFInfo
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- CN103380727A CN103380727A CN2013103171657A CN201310317165A CN103380727A CN 103380727 A CN103380727 A CN 103380727A CN 2013103171657 A CN2013103171657 A CN 2013103171657A CN 201310317165 A CN201310317165 A CN 201310317165A CN 103380727 A CN103380727 A CN 103380727A
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Abstract
The invention discloses a hybrid rice seed production three-line support method, and relates to the technical field of agriculture. The whole process includes the steps: certified seed introduction; test; demonstration; parent purchase; three-line support purification; certified seed production; processing; storage; quality inspection; selling. The hybrid rice seed production three-line support method is characterized in that the specific three-line support purification method includes the steps: 1) selecting individual plants for paired backcross; 2) authenticating fertility and performing plant-to-row test cross; 3) breeding strains and authenticating superiority; 4) finally selecting stock seeds. The hybrid rice seed production three-line support method is simple, easy and high in breeding speed, stability of colonies is ensured, loss of favorable genes is avoided, and the requirement of hybrid rice parent three-line for miscellaneous prevention and pure state preservation is met.
Description
Technical field
The present invention relates to agricultural technology field, be specifically related to a kind of breeding of hybrid rice three series mating method.
Background technology
It is that hybrid rice is produced the next item up significant problem that hybrid rice degenerates, for the good strains of seeds that keeps making up, a large amount of research work have been done to anti-assorted pure keeping method in various places, " one assorted four gardens ", " supporting purification " and every anti-assorted purification measures such as " three are to purify in seven gardens " have been proposed, should how prevent mixing actually purification we domestic several main method have been carried out conscientiously studying and inquiring into.Through practice in a few years, we have proposed the anti-assorted method of purification of " dividing is test cross, supporting purification " on the basis of learning Hunan, river experience.This method is to be undertaken by the Direct Identification to the offspring, that is to say that the purification that hybrid rice three is is to identify by pairing backcrossing, test cross and advantage, directly on the basis of offspring's fertility and restoring force evaluation, to coordinate the main criterion of masterpiece screening.Its main program is: (1) selects the individual plant pairing backcrossing; (2) Fertility identification, the plant test cross; (3) strain breeding, advantage is identified; (4) final election original seed.Father's Academician YUAN Long-ping of hybrid rice has given higher evaluation to said method, and selected technology also meets national current relevant criterion and regulation.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method simple, the breeding of hybrid rice three series mating method that is easy to put into practice.
Technical problem to be solved by this invention realizes by the following technical solutions:
A kind of breeding of hybrid rice three series mating method, the concrete grammar step is: breeding introduce-test-demonstrate-is purchased parent processed (original seed)-three series mating purification-breeding production-processing-storage-quality testing-sale;
The concrete grammar step that described three series mating is purified is as follows, and (1) selects the individual plant pairing backcrossing; (2) Fertility identification, the plant test cross; (3) strain breeding, advantage is identified; (4) final election original seed
1) select the individual plant pairing backcrossing: the individual plant of maintainer, restorer, male sterile line is selected independently to carry out respectively:
1. maintainer line of hybrid rice, restorer individual plant are planted respectively in fertility is even, the field face is smooth, field is easily arranged in filling, be formed into the excellent garden of selecting;
2. primary election: select during heading to have that former kind typicalness is shape, maintainer individual plant neat and consistent, 20 strains of restorer individual plant carry out respectively listing mark, form plant and identify the garden;
3. final election: the maintainer individual plant that primary election is obtained, restorer individual plant carry out respectively 5 pairs of final elections when maturation, enter strain breeding garden, eliminate the maturing stage in appearance with the inconsistent individual plant of former kind typicalness shape;
4. preferred: the preferred seed of strain being bred the garden keeps, and the seed of restorer is used for by the strain single-strain planting, namely is used for the garden plantation of strain pure keeping; A part of seed of maintainer is used for the garden plantation of strain pure keeping, is used for the breeding of male sterile line after all the other seeds mix, and namely is used for breeding stock garden plantation;
2) Fertility identification, the plant test cross
1. hank excellent garden and the plant selected identified that the individual plant evil processed in garden enters the Fertility identification garden, then carry out the plant test cross;
2. preferred 50 strain male sterile line individual plants, enter plant and identify, then carries out the plant breeding;
3) strain breeding, advantage are identified, identify according to advantage, select major clique;
4) final election original seed carries out breeding stock.
The invention has the beneficial effects as follows: the present invention is simple and easy to do, and reproduction speed is fast, has both guaranteed the stability of colony, has avoided the loss of beneficial gene, the anti-assorted pure keeping requirement that meets again Parents In Hybrid Rice three and be.
Description of drawings
Fig. 1 is production routine schematic diagram of the present invention.
Embodiment
For technological means, creation characteristic that the present invention is realized, reach purpose and effect is easy to understand, below in conjunction with concrete diagram, further set forth the present invention.
Embodiment 1
As shown in Figure 1, a kind of breeding of hybrid rice three series mating method, the concrete grammar step is: breeding introduce-test-demonstrate-is purchased parent processed (original seed)-three series mating purification-breeding production-processing-storage-quality testing-sale;
The concrete grammar step that described three series mating is purified is as follows, and (1) selects the individual plant pairing backcrossing; (2) Fertility identification, the plant test cross; (3) strain breeding, advantage is identified; (4) final election original seed
1) select the individual plant pairing backcrossing: the individual plant of maintainer, restorer, male sterile line is selected independently to carry out respectively:
1. maintainer line of hybrid rice, restorer individual plant are planted respectively in fertility is even, the field face is smooth, field is easily arranged in filling, be formed into the excellent garden of selecting;
2. primary election: select during heading to have that former kind typicalness is shape, maintainer individual plant neat and consistent, 20 strains of restorer individual plant carry out respectively listing mark, form plant and identify the garden;
3. final election: the maintainer individual plant that primary election is obtained, restorer individual plant carry out respectively 5 pairs of final elections when maturation, enter strain breeding garden, eliminate the maturing stage in appearance with the inconsistent individual plant of former kind typicalness shape;
4. preferred: the preferred seed of strain being bred the garden keeps, and the seed of restorer is used for by the strain single-strain planting, namely is used for the garden plantation of strain pure keeping; A part of seed of maintainer is used for the garden plantation of strain pure keeping, is used for the breeding of male sterile line after all the other seeds mix, and namely is used for breeding stock garden plantation;
2) Fertility identification, the plant test cross
1. hank excellent garden and the plant selected identified that the individual plant evil processed in garden enters the Fertility identification garden, then carry out the plant test cross;
2. preferred 50 strain male sterile line individual plants, enter plant and identify, then carries out the plant breeding;
3) strain breeding, advantage are identified, identify according to advantage, select major clique;
4) final election original seed carries out breeding stock.
Above demonstration and described basic principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that describes in above-described embodiment and specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (1)
1. breeding of hybrid rice three series mating method, the overall flow step is: breeding introduce-test-demonstrate-is purchased parent processed-three series mating purification-breeding production-processing-storage-quality testing-sale; It is characterized in that: the concrete grammar step that described three series mating is purified is as follows, and 1) selection individual plant pairing backcrossing; 2) Fertility identification, the plant test cross; 3) strain breeding, advantage is identified; 4) final election original seed;
1) select the individual plant pairing backcrossing: the individual plant of maintainer, restorer, male sterile line is selected independently to carry out respectively:
1. maintainer line of hybrid rice, restorer individual plant are planted respectively in fertility is even, the field face is smooth, field is easily arranged in filling, be formed into the excellent garden of selecting;
2. primary election: select during heading to have that former kind typicalness is shape, maintainer individual plant neat and consistent, 20 strains of restorer individual plant carry out respectively listing mark, form plant and identify the garden;
3. final election: the maintainer individual plant that primary election is obtained, restorer individual plant carry out respectively 5 pairs of final elections when maturation, enter strain breeding garden, eliminate the maturing stage in appearance with the inconsistent individual plant of former kind typicalness shape;
4. preferred: the preferred seed of strain being bred the garden keeps, and the seed of restorer is used for by the strain single-strain planting, namely is used for the garden plantation of strain pure keeping; A part of seed of maintainer is used for the garden plantation of strain pure keeping, is used for the breeding of male sterile line after all the other seeds mix, and namely is used for breeding stock garden plantation;
2) Fertility identification, the plant test cross
1. hank excellent garden and the plant selected identified that the individual plant evil processed in garden enters the Fertility identification garden, then carry out the plant test cross;
2. preferred 50 strain male sterile line individual plants, enter plant and identify, then carries out the plant breeding;
3) strain breeding, advantage are identified, identify according to advantage, select major clique;
4) final election original seed carries out breeding stock.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107396827A (en) * | 2016-05-19 | 2017-11-28 | 上海天谷生物科技股份有限公司 | A kind of saving water, resisting drought rice CMS line purifies propagation method |
Citations (4)
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WO2001006845A1 (en) * | 1999-07-25 | 2001-02-01 | State Of Israel/Ministry Of Agriculture | A method for obtaining 100 % male sterile plants to serve as the female parent in hybrid seeds production |
CN101120652A (en) * | 2007-08-14 | 2008-02-13 | 韶关市农业科学研究所 | General purpose purifying method |
CN101218892A (en) * | 2008-01-29 | 2008-07-16 | 四川省农业科学院作物研究所 | Hybridization rice parent strain trilinear pure-preservation and reproducing method |
CN101461325A (en) * | 2009-01-23 | 2009-06-24 | 天津市农作物研究所 | Method for producing original seed for repeat reproduction of japonica rice three-line sterile line strain |
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2013
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001006845A1 (en) * | 1999-07-25 | 2001-02-01 | State Of Israel/Ministry Of Agriculture | A method for obtaining 100 % male sterile plants to serve as the female parent in hybrid seeds production |
CN101120652A (en) * | 2007-08-14 | 2008-02-13 | 韶关市农业科学研究所 | General purpose purifying method |
CN101218892A (en) * | 2008-01-29 | 2008-07-16 | 四川省农业科学院作物研究所 | Hybridization rice parent strain trilinear pure-preservation and reproducing method |
CN101461325A (en) * | 2009-01-23 | 2009-06-24 | 天津市农作物研究所 | Method for producing original seed for repeat reproduction of japonica rice three-line sterile line strain |
Non-Patent Citations (4)
Title |
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应元通: "谈杂交水稻草三系配套提纯复壮标准问题", 《种子》 * |
李成荃主编: "《安徽稻作学》", 31 March 2008, 中国农业出版社 * |
袁隆平等主编: "《杂交水稻育种栽培学》", 30 April 1988, 湖南科学技术出版社 * |
赵华绥: "杂交水稻"三系"提纯复壮技术的商榷", 《种子》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107396827A (en) * | 2016-05-19 | 2017-11-28 | 上海天谷生物科技股份有限公司 | A kind of saving water, resisting drought rice CMS line purifies propagation method |
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