CN102630559A - Breeding method of large rice stigma three-line sterile line - Google Patents
Breeding method of large rice stigma three-line sterile line Download PDFInfo
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- CN102630559A CN102630559A CN2012101457960A CN201210145796A CN102630559A CN 102630559 A CN102630559 A CN 102630559A CN 2012101457960 A CN2012101457960 A CN 2012101457960A CN 201210145796 A CN201210145796 A CN 201210145796A CN 102630559 A CN102630559 A CN 102630559A
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Abstract
The invention discloses a breeding method of a large rice stigma three-line sterile line, belonging to the technical field of rice heterosis utilization. The method comprises the following steps: promoting germination of rice maintainer line till white buds are visible; processing with a chemical mutagen; obtaining a rice mutant with a mutational large stigma character; and breeding into large rice stigma three-line sterile line through hybridization and back-cross methods, wherein the bred large rice stigma three-line sterile line is 2-3 times of ordinary sterile line glumous flower stigma, the chemical mutagen is mixed solution of manganese sulfate and diethyl sulfate, and the mass concentrations of manganese sulfate and diethyl sulfate in the mixed solution are 0.1%. According to the method of the invention, the mutation frequency of the large stigma character can reach 0.02%-0.03%; the exposure rate of the stigma reaches over 85%; combination of the chemical mutagenesis and hybridization and backcross methods for breeding the large rice stigma three-line sterile line has the characteristics of short time and high breeding efficiency, the whole breeding process only needs five-six planting periods and two-three years, and the outcrossing rate and the planting yield of the sterile line can be remarkably improved.
Description
Technical field
The selection that it is male sterile line that the present invention is specifically related to the big column cap three of a kind of paddy rice belongs to the rice heterosis utilization technical field.
Background technology
Paddy rice is the topmost cereal crops of China, and rice yield directly has influence on grain security and social stability.The research of hybrid rice success has improved the output of China paddy rice greatly, yet three is, the different friendship poor performance of two-line sterile line causes the hybrid rice hybrid seed yield low, and breeding cost is high.Breeding of hybrid rice be utilize the pollen of male parent to be scattering into to make it to inseminate on the column cap (gynoecium) of male sterile line grain husk flower solid; The column cap of male sterile line then helps the male parent pollination like the outside (stigma appearing) that is exposed at the grain husk flower; The stigma exposing ratio of male sterile line is the key factor that influences its different friendship performance, and the stigma exposing ratio that improves male sterile line can improve hybrid rice outcrossing seed-setting rate and hybrid seed yield.The high stigma exposure proterties was an important index when therefore, seed selection excellent sterile was.Its column cap of male sterile line with big column cap is prone to expose, and promptly stigma exposing ratio is high, and crossbreeding output is also high than common pillar head male sterile line.Yet, up to now people to the research of the big column cap male sterile line of paddy rice seldom, particularly combining the hybridization big column cap three of means breeding rice of backcrossing through mutagenesis is male sterile line, does not appear in the newspapers so far.If with the conventional hybridization method of backcrossing big column cap proterties is imported male sterile line, the reorganization of Yin Jiyin causes the workload of seed selection big with separating, breeding year limit for length, breed on producing can with big column cap male sterile line generally need the 5-7 year, take time and effort, benefit is low.
Summary of the invention
To the problems referred to above, the selection that to the invention provides the big column cap three of a kind of paddy rice be male sterile line.
For realizing above-mentioned purpose, the present invention adopts following technical scheme:
With paddy rice three be the maintenance line presprouting of seeds to showing money or valuables one carries unintentionally, handle with chemical mutagen then, obtain the rice mutant of big column cap proterties sudden change, breeding the big column cap three of paddy rice through hybridization, the method for backcrossing again is male sterile line; The big column cap three of the paddy rice of seed selection is that male sterile line grain husk chapiter is 2-3 a times big of common male sterile line grain husk chapiter; Said chemical mutagen is the mixed liquor of manganese sulphate and dithyl sulfate, and the mass concentration of manganese sulphate and dithyl sulfate is 0.1% in the mixed liquor.
The concrete steps of said selection are following:
1) water intaking rice three is the seed of maintenance line-B system, and seed soaking, vernalization are after show money or valuables one carries unintentionally, with chemical mutagen immersion treatment 5 hours; Handle back seed clear water rinsing;
2) sowing mutagenesis seed M in the present age
0, with M
0The plant M that plantation grows
1On seed M
2Mix and receive;
3) plantation M
2In generation, is at M
2The column cap that full heading time is taken grain husk is observed screening, and screening obtains huge pillar head process variant and with its mark, the maturing stage individual plant is received and planted;
4) huge pillar head process variant M
3For strain system plantation, at full heading time column cap proterties, the economical character of all mutant and the loose powder proterties of blooming are investigated, select that column cap is big, economical character is close with B and the good mutant of the loose powder proterties of blooming is promptly bred big column cap B and is; Make male parent with big column cap B system, with B be the hybridization of corresponding male sterile line A system, hybridization F
1With big column cap B system backcross BC
1F
1, plantation BC
1F
1, it can isolate big column cap and 2 kinds of sterile strains of common column cap at full heading time, and selecting sterile strain of big column cap and big column cap B system to continue to backcross and promptly breeding the big column cap three of paddy rice is male sterile line.
The present invention is that the maintenance line germination seed obtains huge pillar head process variant through utilizing the mutagenesis method to handle paddy rice three; Obtain single recessive huge pillar tau gene mutant; Select through agronomy and yield traits, the proterties of blooming; Backcross with corresponding male sterile line hybridization, it is close with former male sterile line to breed other proterties again, and having three of big column cap proterties is male sterile line.The method that the big column cap three of breeding rice of the present invention is a male sterile line has required time short (2-3 year), breeding efficiency height, the big column cap male sterile line outcrossing rate height of breeding, the advantage that hybrid seed yield is high.The big column cap three of paddy rice is that male sterile line grain husk chapiter is 2-3 a times big of common male sterile line grain husk chapiter, and stigma exposing ratio reaches more than 85%, can significantly improve the outcrossing rate and the hybrid seed yield (hybrid seed yield can improve 5-10%) of male sterile line.The breeding method that the big column cap three of mutagenesis breeding rice is a male sterile line is simple, efficient, the breeding time limit is short, breeding efficiency is high, profitable.
Advantage of the present invention and effect:
1) to combine the hybridization big column cap three of method breeding rice of backcrossing be that the male sterile line required time is short, breeding efficiency is high in mutagenesis, and whole breeding process only needs 5-6 plantation generation, 2-3 year.
2) the big column cap three of the paddy rice of breeding is that male sterile line is compared with former pillar head male sterile line; Economical character, fertility proterties are similar with coordinate force; Ability keeps characteristics such as high yield, high-combining ability, the fertility of former male sterile line is good, has the advantage of stigma exposing ratio height (more than 85%), outcrossing rate and hybrid seed yield high (hybrid seed yield can improve 5-10%) again.
Embodiment
Embodiment 1
Water intaking rice three is maintenance line II-32B seed 600 grams; Soaked seed 24 hours; 30 degree vernalization 18-24 hour to seed buds showed money or valuables one carries unintentionally, with chemical mutagen manganese sulphate and dithyl sulfate mixed liquor (mass concentration of manganese sulphate and dithyl sulfate is 0.1%) immersion treatment 5 hours; Handle back seed clear water rinsing 2 times, sowing; M
1Seed (M on the plant
2) mix to receive, drying; Plantation M
2In generation, is at M
2The column cap that take grain husk flowering stage is observed screening, and screening obtains huge pillar head process variant and with its mark of listing, the maturing stage individual plant is received and planted; Big column cap II-32B mutant M
3For the plantation of strain system; Compare with II-32B; At full heading time column cap proterties, the economical character of all mutant and the loose powder proterties of blooming are investigated; Select the big column cap II-32B mutant that column cap is big, The Characters is close with II-32B and the loose powder proterties of blooming is good to make male parent, with II-32A hybridization, hybridization F
1With big column cap II-32B backcross BC
1F
1, plantation BC
1F
1, it can isolate big column cap and 2 kinds of sterile strains of common pillar head at full heading time, and selecting sterile strain of big column cap and big column cap II-32B to continue to backcross and promptly breeding the big column cap three of paddy rice is male sterile line--big column cap II-32A.The stigma exposing ratio of big column cap II-32A reaches 89%, exceeds 41 percentage points than 48% of II-32A.With big column cap II-32A and bright extensive 86 preparing hybrid rice, its hybrid seed yield is higher by 7.8% than II-32A and bright extensive 86 preparing hybrid rice.
Embodiment 2
Water intaking rice three is maintenance line Zhenshan 97B seed 550 grams; Soaked seed 24 hours; 30 degree vernalization 18-24 hour to seed buds showed money or valuables one carries unintentionally, with chemical mutagen manganese sulphate and dithyl sulfate mixed liquor (mass concentration of manganese sulphate and dithyl sulfate is 0.1%) immersion treatment 5 hours; Handle back seed clear water rinsing 2 times, sowing; M
1Seed (M on the plant
2) mix and receive drying; Plantation M
2In generation, is at M
2The column cap that take grain husk flowering stage is observed screening, and screening obtains huge pillar head process variant and with its mark of listing, the maturing stage individual plant is received and planted; Big column cap Zhenshan 97B mutant M
3For the plantation of strain system; Compare with Zhenshan 97B; At full heading time column cap proterties, the economical character of all mutant and the loose powder proterties of blooming are investigated; Select the big column cap Zhenshan 97B mutant that column cap is big, The Characters is close with Zhenshan 97B and the loose powder proterties of blooming is good to make male parent, with Zhenshan 97a hybridization, hybridization F
1With big column cap Zhenshan 97B backcross BC
1F
1, plantation BC
1F
1, it can isolate big column cap and 2 kinds of sterile strains of common pillar head at full heading time, and selecting sterile strain of big column cap and big column cap Zhenshan 97B to continue to backcross and promptly breeding the big column cap three of paddy rice is male sterile line--big column cap Zhenshan 97a.The stigma exposing ratio of big column cap Zhenshan 97a reaches 91%, exceeds 39 percentage points than 52% of Zhenshan 97a.With big column cap Zhenshan 97a and bright extensive 63 preparing hybrid rice, its hybrid seed yield is higher by 8.4% than Zhenshan 97a and bright extensive 63 preparing hybrid rice.
Embodiment 3
Water intaking rice three is maintenance line D297B seed 580 grams; Soaked seed 24 hours; 30 degree vernalization 18-24 hour to seed buds showed money or valuables one carries unintentionally, with chemical mutagen manganese sulphate and dithyl sulfate mixed liquor (mass concentration of manganese sulphate and dithyl sulfate is 0.1%) immersion treatment 5 hours; Handle back seed clear water rinsing 2 times, sowing; M
1Seed (M on the plant
2) mix and receive drying; Plantation M
2In generation, is at M
2The column cap that take grain husk flowering stage is observed screening, and screening obtains huge pillar head process variant and with its mark of listing, the maturing stage individual plant is received and planted; Big column cap D297B mutant M
3For the plantation of strain system; Compare with D297B; At full heading time column cap proterties, the economical character of all mutant and the loose powder proterties of blooming are investigated; Select the big column cap D297B mutant that column cap is big, The Characters is close with D297B and the loose powder proterties of blooming is good to make male parent, with D297A hybridization, hybridization F
1With big column cap D297B backcross BC
1F
1, plantation BC
1F
1, it can isolate big column cap and 2 kinds of sterile strains of common pillar head at full heading time, and selecting sterile strain of big column cap and big column cap D297B to continue to backcross and promptly breeding the big column cap three of paddy rice is male sterile line--big column cap D297A.The stigma exposing ratio of big column cap D297A reaches 93%, exceeds 38 percentage points than 55% of D297A.With big column cap D297A and FR315 preparing hybrid rice, its hybrid seed yield is higher by 6.7% than D297A and FR315 preparing hybrid rice.
The above is merely preferred embodiment of the present invention, and all equalizations of doing according to claim of the present invention change and modify, and all should belong to covering scope of the present invention.
Claims (2)
1. selection that the big column cap three of paddy rice is a male sterile line; It is characterized in that: with paddy rice three is that the maintenance line presprouting of seeds is to showing money or valuables one carries unintentionally; Handle with chemical mutagen then, obtain the rice mutant of big column cap proterties sudden change, breeding the big column cap three of paddy rice through hybridization, the method for backcrossing again is male sterile line; The big column cap three of the paddy rice of seed selection is that male sterile line grain husk chapiter is 2-3 a times big of common male sterile line grain husk chapiter; Said chemical mutagen is the mixed liquor of manganese sulphate and dithyl sulfate, and the mass concentration of manganese sulphate and dithyl sulfate is 0.1% in the mixed liquor.
2. the selection that the big column cap three of paddy rice according to claim 1 is a male sterile line, it is characterized in that: the concrete steps of said selection are following:
1) water intaking rice three is the seed of maintenance line-B system, and seed soaking, vernalization are after show money or valuables one carries unintentionally, with chemical mutagen immersion treatment 5 hours; Handle back seed clear water rinsing;
2) sowing mutagenesis seed M in the present age
0, with M
0The plant M that plantation grows
1On seed M
2Mix and receive;
3) plantation M
2In generation, is at M
2The column cap that full heading time is taken grain husk is observed screening, and screening obtains huge pillar head process variant and with its mark, the maturing stage individual plant is received and planted;
4) huge pillar head process variant M
3For strain system plantation, at full heading time column cap proterties, the economical character of all mutant and the loose powder proterties of blooming are investigated, select that column cap is big, economical character is close with B and the good mutant of the loose powder proterties of blooming is promptly bred big column cap B and is; Make male parent with big column cap B system, with B be the hybridization of corresponding male sterile line A system, hybridization F
1With big column cap B system backcross BC
1F
1, plantation BC
1F
1, it can isolate big column cap and 2 kinds of sterile strains of common column cap at full heading time, and selecting sterile strain of big column cap and big column cap B system to continue to backcross and promptly breeding the big column cap three of paddy rice is male sterile line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101457960A CN102630559A (en) | 2012-05-12 | 2012-05-12 | Breeding method of large rice stigma three-line sterile line |
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|---|---|---|---|
| CN2012101457960A CN102630559A (en) | 2012-05-12 | 2012-05-12 | Breeding method of large rice stigma three-line sterile line |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103125368A (en) * | 2013-03-13 | 2013-06-05 | 福建农林大学 | Breeding method of insect-resistant big-stigma three-line sterile line |
| CN114431246A (en) * | 2022-01-17 | 2022-05-06 | 浙江省农业科学院 | Composite reagent for improving stigma exposure rate of japonica rice three-line sterile line and application thereof |
-
2012
- 2012-05-12 CN CN2012101457960A patent/CN102630559A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103125368A (en) * | 2013-03-13 | 2013-06-05 | 福建农林大学 | Breeding method of insect-resistant big-stigma three-line sterile line |
| CN103125368B (en) * | 2013-03-13 | 2014-06-25 | 福建农林大学 | Breeding method of insect-resistant big-stigma three-line sterile line |
| CN114431246A (en) * | 2022-01-17 | 2022-05-06 | 浙江省农业科学院 | Composite reagent for improving stigma exposure rate of japonica rice three-line sterile line and application thereof |
| CN114431246B (en) * | 2022-01-17 | 2023-09-01 | 浙江省农业科学院 | Composite medicament for improving stigma exposure rate of japonica rice three-line sterile line and application thereof |
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Application publication date: 20120815 |