CN114431246A - Composite reagent for improving stigma exposure rate of japonica rice three-line sterile line and application thereof - Google Patents
Composite reagent for improving stigma exposure rate of japonica rice three-line sterile line and application thereof Download PDFInfo
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Abstract
The invention discloses a compound medicament for improving stigma exposure rate of a japonica rice three-line sterile line and application thereof, wherein the compound medicament comprises the following components: 1.5-2.5 mg/L6-benzylaminopurine, 1.5-2.5mg/L kinetin, 4-6g/L methyl jasmonate, 250-350mg/L gibberellin 3, 25-35mg/L potassium dihydrogen phosphate, 1.0-1.4g/L borax and 2.8-3.2g/L diethylenetriamine. The compound medicament is prepared by combining 6-benzylaminopurine, kinetin, methyl jasmonate and gibberellin 3, is safe and pollution-free, can improve the stigma exposure rate of the japonica rice sterile line by more than 13 percent, and has obvious effects of improving the seed setting rate and the yield of hybrid seed production; compared with the clear water control, the stigma exsertion is improved by nearly 10 percent, and the hybridization maturing rate is improved by nearly 20 percent.
Description
Technical Field
The invention relates to the technical field of rice breeding, in particular to a compound medicament for improving stigma exposure rate of a japonica rice three-line sterile line and application thereof.
Background
Rice (Oryza sativa L.) is one of the three most important food crops in China and even all over the world, accounts for 25% of the total food production in the world, and rice is taken as staple food by people above 1/2 in the world. Therefore, the rice yield is related to food safety in China and even in the world, and the improvement of the yield per unit becomes one of the important targets of rice breeding scientists.
The rice field-sterile line is discovered by Yuanlongping academician in 1964, the rice three-line matching is successfully realized under the common efforts of national breeding technologists, and a series of new hybrid rice varieties are cultivated and planted in a large area. The application of the major scientific and technological achievement brings a leap to the rice production in China, so that the yield per unit of rice is improved by about 20 percent on the basis of short-stalk improved varieties, and the method makes a great contribution to the development of grain production. Then, Chinese scientists successively breed two lines of hybrid rice and hybrid rice between subspecies of indica rice and japonica rice, particularly the latter is known as 5 th generation (5G) rice, is suitable for planting in ecological regions of indica rice and japonica rice, and breaks the boundary of planting regions of indica rice and japonica rice. Moreover, the yield of indica-japonica hybrid rice is particularly obvious, and the scientific community generally proves that the yield of the rice is 15-30% higher than that of the existing three-line and two-line hybrid rice, and the rice has the characteristics of lodging resistance, cold resistance and the like.
At present, China has cultivated indica-japonica hybrid rice series of Yongyou, Zhejiang-you and Chun-you. However, the sterile line of japonica rice has low stigma exposure rate and has a poor flowering time with the indica restorer line, which makes seed production difficult. Research shows that the sterile line stigma exposure rate is high, more pollen can be accepted, and the outcrossing maturing rate and the stigma exposure rate are in obvious positive correlation (such as plum, sea and the like, breeding and utilization of the Liaoning 143A hybrid rice sterile line with early flowering, high exposure rate and larger stigma japonica rice, agricultural science and technology communication 2020, 2: 234-. Jiangxilu et al (Jiangxilu et al, influence of three nutrient hormone compound agents on exposure rate of stigmas of hybrid rice, plant physiological communication, 2008, 44 (4): 630-. While Lukai et al (Lukai et al, influence of fertilization and hormone on stigma exsertion rate and maturing rate of rice sterile line, Anhui agricultural science, 2003.31 (4): 641 and 642) successfully improve stigma exsertion rate of Xieqingzao A and Zhenshan 97A of indica rice sterile line and increase seed production yield by using Jiu Diozi, potassium dihydrogen phosphate and Huatiaoling and other medicaments.
However, so far, no related technical report for improving stigma exposure rate of a sterile line of japonica rice so as to improve seed production yield of indica-japonica hybrid rice exists. Therefore, the technical method for improving the stigma exposure rate of the japonica rice sterile line has great application value in the seed production application of indica-japonica hybrid rice.
Disclosure of Invention
The invention provides a compound medicament for improving stigma exposure rate of a three-line sterile line of japonica rice and application thereof.
The specific technical scheme is as follows:
a compound medicament for improving stigma exposure rate of a japonica rice three-line sterile line comprises: 1.5-2.5 mg/L6-benzylaminopurine, 1.5-2.5mg/L kinetin, 4-6g/L methyl jasmonate, 250-350mg/L gibberellin 3, 25-35mg/L potassium dihydrogen phosphate, 1.0-1.4g/L borax and 2.8-3.2g/L diethylenetriamine.
The plant growth regulators such as 6-benzylaminopurine, kinetin, gibberellin and the like in the invention can not be used alone or in combination to increase stigma exsertion and fructification rate; methyl jasmonate alone or in combination with gibberellin or in combination with 6-benzylaminopurine and kinetin can not improve stigma exsertion rate, but can improve hybrid seed setting rate, which indicates that methyl jasmonate has the effect of improving seed setting rate of hybrid seed production; when 4 hormones (6-benzylaminopurine, kinetin, methyl jasmonate and gibberellin) are used in combination, the stigma exposure rate of the sterile line can be improved, and the hybrid seed production rate can be further improved.
The compound medicament also comprises an organic solvent; the organic solvent is Tween20 with a volume fraction of 0.02%.
Further, the compound medicament consists of the following components: 2 mg/L6-benzylaminopurine, 2mg/L kinetin, 5g/L methyl jasmonate, 300mg/L gibberellin 3, 30mg/L monopotassium phosphate, 1.2g/L borax, 3g/L diethylenetriamine and Tween20 with the volume fraction of 0.02%.
The invention provides application of the compound medicament for improving the stigma exposure rate of the three-line sterile line of japonica rice in indica-japonica hybrid seed production.
The invention also provides a method for improving stigma exposure rate of the japonica rice three-line sterile line, which comprises the following steps: when the ear-picking rate of the three-line sterile line of the japonica rice reaches 5 percent, the compound medicament is sprayed on the ear of the sterile line.
Further, the spraying frequency of the compound medicament is one time or two times; the two times are separated by one day.
Compared with the prior art, the invention has the following beneficial effects:
(1) the compound medicament is prepared by combining 6-benzylaminopurine, kinetin, methyl jasmonate and gibberellin 3, is safe and pollution-free, can improve the stigma exposure rate of the japonica rice sterile line by more than 13 percent, and has obvious effects of improving the seed setting rate and the yield of hybrid seed production; compared with the clear water control, the stigma exsertion is improved by nearly 10 percent, and the hybridization maturing rate is improved by nearly 20 percent.
(2) The compound medicament has the technical advantages of simple preparation, convenient use, simple and convenient operation in spraying, remarkable effect and the like.
Drawings
FIG. 1 is the effect of the compound chemical agent in example 1 on the maturing rate of the sterile line of japonica rice.
Detailed Description
The present invention will be further described with reference to the following specific examples, which are only illustrative of the present invention, but the scope of the present invention is not limited thereto.
Example 1
1. Preparation of pharmaceutical agents
(1) Compound medicament: 2mg/L of 6-benzylaminopurine (6-BA) dissolved by 1ml of 1N NaOH; 2mg/L Kinetin (KT) is dissolved by 1ml of 1N NaOH; dissolving methyl jasmonate (JA-Me) at a ratio of 5g/L in ethanol and Tween 20; dissolving gibberellin 3(GA3)300mg/L in anhydrous alcohol; potassium dihydrogen phosphate (KH)2PO4)30mg/L, dissolving with water; dissolving 1.2g/L of borax and 3g/L of diethylenetriamine in boiling water; these dissolved reagents were mixed and 0.02% (v/v) Tween20 was added.
(2) Control agent 1: dissolving methyl jasmonate (JA-Me) at a ratio of 5g/L in ethanol and Tween 20; potassium dihydrogen phosphate (KH)2PO4)30mg/L, dissolving with water; dissolving 1.2g/L of borax and 3g/L of diethylenetriamine in boiling water; 0.02% (v/v) Tween20 was added to the solubilized reagent.
(3) Control agent 2: 2mg/L of 6-benzylaminopurine (6-BA) dissolved by 1ml of 1N NaOH; 2mg/L Kinetin (KT) is dissolved by 1ml of 1N NaOH; potassium dihydrogen phosphate (KH)2PO4)30mg/L, dissolving with water; dissolving 1.2g/L of borax and 3g/L of diethylenetriamine in boiling water; these dissolved reagents were mixed and 0.02% (v/v) Tween20 was added.
(4) Control agent 3: dissolving gibberellin 3(GA3)300mg/L in anhydrous alcohol; potassium dihydrogen phosphate (KH)2PO4)30mg/L, dissolving with water; dissolving 1.2g/L of borax and 3g/L of diethylenetriamine in boiling water; these dissolved reagents were mixed and 0.02% (v/v) Tween20 was added.
(5) Control agent 4: 2mg/L of 6-benzylaminopurine (6-BA) dissolved by 1ml of 1N NaOH; 2mg/L Kinetin (KT) is dissolved by 1ml of 1N NaOH; dissolving gibberellin 3(GA3)300mg/L in anhydrous alcohol; potassium dihydrogen phosphate (KH)2PO4)30mg/L, dissolving with water; dissolving 1.2g/L of borax and 3g/L of diethylenetriamine in boiling water; these dissolved reagents were mixed and 0.02% (v/v) Tween20 was added.
(6) Control agent 5: 2mg/L of 6-benzylaminopurine (6-BA) in a volume of 1ml of 1NDissolving NaOH; 2mg/L Kinetin (KT) is dissolved by 1ml of 1N NaOH; dissolving methyl jasmonate (JA-Me) at a ratio of 5g/L in ethanol and Tween 20; potassium dihydrogen phosphate (KH)2PO4)30mg/L, dissolving with water; dissolving 1.2g/L of borax and 3g/L of diethylenetriamine in boiling water; these dissolved reagents were mixed and 0.02% (v/v) Tween20 was added.
(7) Control agent 6: dissolving methyl jasmonate (JA-Me) at a ratio of 5g/L in ethanol and Tween 20; dissolving gibberellin 3(GA3)300mg/L in anhydrous alcohol; potassium dihydrogen phosphate (KH)2PO4)30mg/L, dissolving with water; dissolving 1.2g/L of borax and 3g/L of diethylenetriamine in boiling water; these dissolved reagents were mixed and 0.02% (v/v) Tween20 was added.
(8) Blank control: 0.02% (v/v) Tween20 in water.
2. Determination of stigma Exposure Rate
The materials used in the experiment are japonica rice three-line sterile line Zhe Xin A and Huazhong 1A.
Planting under field conditions, managing by conventional fertilizer and water, respectively spraying the medicament prepared in the step 1 for 1 time when 5% of rice materials are subjected to ear sprouting, and then spraying the medicament for 1 time every 1 day. And counting the number of glume flowers with exposed stigmas on the 2 nd day of rice flowering. The stigma exposure rate is the number of stigma-exposed glumes/the number of all glumes in a single inflorescence x 100%. Each experiment was repeated 5 times. Significant difference analysis was performed using t-test.
The results show that the compound medicament can obviously improve the stigma exposure rate by nearly 10 percent compared with a blank control (Table 1).
TABLE 1 Effect of different chemical Agents on Rice glume stigma exsertion
3. Determination of seed set percentage
The materials used in the experiment are japonica rice three-line sterile line Zhejiang New A and Huazhong 1A, and indica rice recovery line Zhejiang Hui 9326.
Planting under field conditions, and according to a recovery system: the row ratio of the sterile line is 1: 6 planting rice material. And (3) respectively spraying the medicament prepared in the step (1) for 1 time when 5% of rice materials are in ear sprouting, spraying the medicament for 1 time every other 1 day, and simultaneously, matching with manual normal pollen dispelling. And counting the seed setting rate of the sterile line at 40 days after heading and flowering, and repeating each experiment for 5 times. Significant difference analysis was performed using t-test.
The results show that the compound medicament can significantly improve the setting rate by about 20 percent compared with the blank control (figure 1).
Claims (6)
1. A compound medicament for improving stigma exposure rate of a three-line sterile line of japonica rice is characterized by comprising: 1.5-2.5 mg/L6-benzylaminopurine, 1.5-2.5mg/L kinetin, 4-6g/L methyl jasmonate, 250-350mg/L gibberellin 3, 25-35mg/L potassium dihydrogen phosphate, 1.0-1.4g/L borax and 2.8-3.2g/L diethylenetriamine.
2. The compound agent for increasing stigma exposure rate of japonica rice three-line sterile line according to claim 1, further comprising an organic solvent; the organic solvent is Tween20 with a volume fraction of 0.02%.
3. The compound medicament for improving stigma exposure rate of japonica rice three-line sterile lines as claimed in claim 1, which is characterized by comprising the following components: 2 mg/L6-benzylaminopurine, 2mg/L kinetin, 5g/L methyl jasmonate, 300mg/L gibberellin 3, 30mg/L monopotassium phosphate, 1.2g/L borax, 3g/L diethylenetriamine and Tween20 with the volume fraction of 0.02%.
4. The use of the compound agent for improving stigma exposure rate of japonica rice three-line sterile line as claimed in any one of claims 1 to 3 in indica-japonica hybrid seed production.
5. A method for improving stigma exposure rate of a japonica rice three-line sterile line is characterized by comprising the following steps: spraying the compound medicament of any one of claims 1 to 3 to the ear of the sterile line when the ear-sprouting rate of the three-line sterile line of japonica rice reaches 5%.
6. The method for improving stigma exsertion rate of japonica rice three-line sterile line as claimed in claim 5, wherein the spraying frequency of the compound agent is one or two; the two times are separated by one day.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0233040A2 (en) * | 1986-02-04 | 1987-08-19 | Ajinomoto Co., Inc. | Stigma of crocus sativus L. and method for the production thereof |
| US5217897A (en) * | 1986-09-20 | 1993-06-08 | Ohta's Isan Co., Ltd. | Process for culturing saffron stigma tissues |
| CN1843087A (en) * | 2005-12-27 | 2006-10-11 | 辽宁省稻作研究所 | High stigma exposure rate japonica rice sterile line selection and breeding technology |
| CN101223886A (en) * | 2008-02-01 | 2008-07-23 | 四川省农业科学院生物技术核技术研究所 | Drug of increasing stigma exposing ratio of sterile line rice and preparing method thereof |
| CN102630559A (en) * | 2012-05-12 | 2012-08-15 | 福建农林大学 | Breeding method of large rice stigma three-line sterile line |
| CN113105285A (en) * | 2021-03-26 | 2021-07-13 | 浙江大学 | Female parent flowering time regulator for japonica indica rice hybrid seed production and method for regulating flowering time |
-
2022
- 2022-01-17 CN CN202210049921.1A patent/CN114431246B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0233040A2 (en) * | 1986-02-04 | 1987-08-19 | Ajinomoto Co., Inc. | Stigma of crocus sativus L. and method for the production thereof |
| US5217897A (en) * | 1986-09-20 | 1993-06-08 | Ohta's Isan Co., Ltd. | Process for culturing saffron stigma tissues |
| CN1843087A (en) * | 2005-12-27 | 2006-10-11 | 辽宁省稻作研究所 | High stigma exposure rate japonica rice sterile line selection and breeding technology |
| CN101223886A (en) * | 2008-02-01 | 2008-07-23 | 四川省农业科学院生物技术核技术研究所 | Drug of increasing stigma exposing ratio of sterile line rice and preparing method thereof |
| CN102630559A (en) * | 2012-05-12 | 2012-08-15 | 福建农林大学 | Breeding method of large rice stigma three-line sterile line |
| CN113105285A (en) * | 2021-03-26 | 2021-07-13 | 浙江大学 | Female parent flowering time regulator for japonica indica rice hybrid seed production and method for regulating flowering time |
Non-Patent Citations (2)
| Title |
|---|
| SUSILAWATI P N, SURAHMAN M, PURWOKO B S, ET AL.: "Effect of GA3 concentration on hybrid rice seed production in Indonesia", INTERNATIONAL JOURNAL OF APPLIED SCIENCE AND TECHNOLOGY, vol. 4, no. 2, pages 143 - 148 * |
| 吕凯等: "施肥和激素对水稻不育系柱头外露率和结实率的影响", 安徽农业科学, vol. 31, no. 4, pages 641 - 642 * |
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