CN105494372B - Growth regulator and the application of breeding of hybrid rice and sterile line breeding yield can be improved - Google Patents
Growth regulator and the application of breeding of hybrid rice and sterile line breeding yield can be improved Download PDFInfo
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- CN105494372B CN105494372B CN201510917621.0A CN201510917621A CN105494372B CN 105494372 B CN105494372 B CN 105494372B CN 201510917621 A CN201510917621 A CN 201510917621A CN 105494372 B CN105494372 B CN 105494372B
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- breeding
- growth regulator
- sterile line
- hybrid rice
- zeatin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Botany (AREA)
- Genetics & Genomics (AREA)
- Developmental Biology & Embryology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides a kind of growth regulator for improving breeding of hybrid rice and sterile line breeding yield, and the growth regulator includes following component：2,4 epi-brassinolides, 3 heteroauxins, zeatin and water, pH value 5.8~6.5.The growth regulator that the present invention is provided, in breeding of hybrid rice and sterile line breeding, the percentage of earbearing tiller of sterile line can be improved and then increase number of productive ear, floret degeneration is reduced and then increase grain number per spike, promote to be in the milk and then setting percentage and/or increase mass of 1000 kernel is improved, hybrid seed production yields and sterile line breeding yield is finally improved.Its compound method is simple and easy to apply, and product stability is good, both effectiveness strong, safe and reliable, has no side effect, suitable for being produced in hybrid rice seeds in popularization and application.
Description
Technical field
The present invention relates to plant growth regulator technical field, specifically, breeding of hybrid rice can be improved by being related to one kind
Growth regulator and its application with sterile line breeding yield.
Background technology
China is eating rice production state and country of consumption maximum in the world, and more than 60% population is using rice as staple food.Hybrid
The per unit area yield and total yield of the production of China paddy rice is greatly improved in the utilization of advantage, to ensure that it is huge that China's grain security has been made
Big contribution.
The important channel of rice heterosis utilization is hybrid seeding, but under normal circumstances, hybrid seeding and sterile line
The actual production of breeding is relatively low, greatly governs the development of hybrid rice.
In addition, paddy rice fertilizer requirement in planting process is big, but often utilization rate is low for the traditional fertilising of China, and soil is made
Into injury.Plant growth regulator is used for foliage-spray, and consumption is few, instant effect, and low to the harm of environment, is more suitable for extensive
Popularization and application.
The content of the invention
It is an object of the invention to provide a kind of growth regulator for improving the paddy rice cross breeding production of hybrid seeds and sterile line breeding yield
And its application.
In order to realize the object of the invention, a kind of life for improving breeding of hybrid rice and sterile line breeding yield of the invention
Long conditioning agent, the growth regulator includes following component：2,4- epi-brassinolides, 3-indolyl acetic acid, zeatin and water, pH
Value 5.8~6.5.
Preferably, the composition of the growth regulator be 1L water in contain 2,4- epi-brassinolides 0.05-0.1mg, 3-
Heteroauxin 30mg and zeatin 0.5-1.0mg.
It is highly preferred that the composition of the growth regulator (formula I) is to contain 2,4- epi-brassinolides in 1L water
0.1mg, 3-indolyl acetic acid 30mg and zeatin 0.5mg.In second stalk of sterile line and clever flower primordium idiophase (ear differentiation
The third phase) spray in sterile line blade face.
It is highly preferred that the composition of the growth regulator (formula II) is to contain 2,4- epi-brassinolides in 1L water
0.05mg, 3-indolyl acetic acid 30mg and zeatin 1.0mg.In sterile line pollen mother cell formation stage (the ear differentiation fifth phase) spray
Impose on sterile line blade face.
The present invention also provides the preparation method of the growth regulator, and 2,4- epi-brassinolides, 3- Yin are weighed in proportion
Indolylbutyric acid and zeatin, first dissolve above-mentioned each component (alcohol helps to increase the dissolubility of each raw material) with a small amount of 95% alcohol,
It is then dissolved in water, adjusts pH value 5.8~6.5, be then settled to 1L.
The present invention further provides application of the growth regulator in the paddy rice cross breeding production of hybrid seeds and sterile line breeding is improved,
Concrete mode is as follows：
The field planting method of breeding of hybrid rice and sterile line breeding is：The plantation specification of sterile line be 16.5cm ×
20cm, individual plant transplanting, parental is 2:10, operation aisle is removed, every mu is planted male parent 1500-2000 caves, plants female parent
13000-15000 caves.It can be adjusted as needed during practical application.
In sterile line Secondary branch former base and clever flower primordium idiophase, i.e. the ear differentiation third phase, formula I growth is sprayed
Conditioning agent, every mu sprays 40-60L, if rained after spraying in two hours, fills spray once.
Using the method for foliage-spray, in sterile line pollen mother cell formation stage, i.e. the ear differentiation fifth phase, formula is sprayed
II growth regulator, every mu sprays 40-60L, if rained after spraying in two hours, fills spray once.
Wherein, rice material used in breeding of hybrid rice includes but is not limited to sterile line Toyota 1A, restorer Toyota
1B, maintainer RC6.
The growth regulator that the present invention is provided, in breeding of hybrid rice and sterile line breeding, can improve sterile line into
Fringe rate and then increase number of productive ear, reduction floret degeneration and then increase grain number per spike, promotion grouting and then raising setting percentage and/or increasing
Plus mass of 1000 kernel, finally improve hybrid seed production yields and sterile line breeding yield.Its compound method is simple and easy to apply, product stability
It is good, it is both effectiveness strong, it is safe and reliable, have no side effect, suitable for being produced in hybrid rice seeds in popularization and application.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.Unless otherwise specified, embodiment
In the conventional meanses that are well known to those skilled in the art of used technological means, raw materials used is commercial goods.
Embodiment 1 can improve growth regulator and its application of breeding of hybrid rice and sterile line breeding yield
The formula (content per 1L) of the present embodiment growth regulator：2,4- epi-brassinolide 0.1mg, 3-indolyl acetic acid
30mg, zeatin 0.5mg, pH value 5.8~6.5.
The preparation method of growth regulator：2,4- epi-brassinolides, 3-indolyl acetic acid and zeatin are weighed in proportion, first
Above-mentioned each component is dissolved with a small amount of 95% alcohol, is then dissolved in water, pH value 5.8~6.5 is adjusted, is then settled to 1L.
The field planting method (can be adjusted as needed during practical application) of breeding of hybrid rice and breeding：Infertility
The plantation specification of system is 16.5cm × 20cm, and individual plant transplanting, parental is 2:10, operation aisle is removed, every mu is planted father
This 1500-2000 caves, plant maternal 13000-15000 caves.
Growth regulator application process：Second stalk and grain husk are reached in the main fringe breeding time of about 80% sterile line plant
During the flower primordium idiophase, every mu sprays the growth regulator of the above-mentioned formulas of 40-60L in sterile line blade face.If two hours after spraying
It is interior to rain, then fill spray once.In female parent heading 30%, the sterile line of each cell sprays nine by the normal production of hybrid seeds and breeding requirement
20 (gibberellin).
Experiment sterile line material used is Toyota 1A, in sterile line Toyota 1A Post flowerings, respectively with restorer Toyota 1B and
Maintainer RC6 combos are hybridized.Control is not in addition to spraying the growth regulator, and remaining experimental condition is identical.Experiment is used
RANDOMIZED BLOCK DESIGN, repeatedly district's groups, the results are shown in Table 1 and table 2 twice.
As a result show：In terms of number of productive ear, in cross combination Toyota 1A × Toyota 1B, the number of productive ear of processing is deposited with compareing
In significant difference, wherein the number of productive ear handled improves 12.6% than control.In cross combination Toyota 1A × RC6, effectively
Spike number is with compareing and in the absence of significant difference.In terms of total grain panicle number, there is significant difference in processing with compareing, wherein, processing
Per total grain panicle number 11.4% is improved than control.
In terms of mass of 1000 kernel, in cross combination Toyota 1A × Toyota 1B, handle with the mass of 1000 kernel compareed without marked difference.
In cross combination Toyota 1A × RC6, mass of 1000 kernel improves 7.3% compared to control, and difference reaches significantly.
In terms of theoretical yield, cross combination Toyota 1A × Toyota 1B and combination Toyota 1A × RC6 hybrid seed yield compare
Control is high, and reaches significant difference.Wherein, cross combination Toyota 1A × Toyota 1B hybrid seed yield is than compareing high by 22.7%, group
The hybrid seed yield for closing Toyota 1A × RC6 is higher by 34.1% than compareing.
Table 1 handles the influence to combining Toyota 1A × Toyota 1B breeding yield components
Note：Different lowercase letter indication differences are up to 5% level of signifiance in same row, and same letter represents that difference is not notable.
Table 2 handles the influence to combining Toyota 1A × RC6 hybrid seed yield constituent elements
Note：Different lowercase letter indication differences are up to 5% level of signifiance in same row, and same letter represents that difference is not notable.
Benefit estimation：
Cost estimation：According to Alibaba's net price, 90% purity of Shanghai Vegcides Bio-farm's production
2,4- 340 yuan of epi-brassinolide 500g prices, it is monovalent 0.68 yuan equivalent to every g；Suzhou day can trade Co., Ltd production
100g fills 380 yuan of (98% purity) 3-indolyl acetic acid price, monovalent 3.8 yuan equivalent to every g；The many good fortune Buddhist nun biotechnologies of Nanjing Olympic have
100mg 185 yuan of tissue culture reagent zeatin (purity 99%) prices of dress of limit company production, it is monovalent 1.85 yuan equivalent to every mg.Press
According to average mu consumption 50L meters, every mu is using reagent cost：2,4- epi-brassinolides 0.1mg × 50/1000 × 0.68+3-
Heteroauxin 30mg × 50/1000 × 3.8+ zeatin 0.5mg × 50 × 1.85=0.003+5.7+46.25=51.753 members.
Income estimation：Sterile line breeding, every mu of breeding increases production 23.8kg, and company purchases 40 yuan/kg from peasant household, increases income 952
Member, removes about 52 yuan of reagent cost, and the 2-3 buckets of 50L about conventional nebulizers, 20 yuan of labour cost, peasant household has a net increase of 880 yuan of receipts.It is miscellaneous
The rice production of hybrid seeds is handed over, every mu of volume increase 34.2kg, company increases income 1026 yuan, remove reagent cost 52 months and people from peasant household 30 yuan/kg of purchase
20 yuan of expenses of labour, peasant household has a net increase of 954 yuan of receipts.
Embodiment 2 can improve growth regulator and its application of breeding of hybrid rice and sterile line breeding yield
The formula (content per 1L) of the present embodiment growth regulator：Contain 2,4- epi-brassinolides in 1L water
0.05mg, 3-indolyl acetic acid 30mg, zeatin 1.0mg, pH value 5.8~6.5.
The preparation method of growth regulator：2,4- epi-brassinolides, 3-indolyl acetic acid and zeatin are weighed in proportion, first
Above-mentioned each component is dissolved with a small amount of 95% alcohol, is then dissolved in water, pH value 5.8~6.5 is adjusted, is then settled to 1L.
The field planting method (can be adjusted as needed during practical application) of breeding of hybrid rice and breeding：Infertility
The plantation specification of system is 16.5cm × 20cm, and individual plant transplanting, parental is 2:10, operation aisle is removed, every mu is planted father
This 1500-2000 caves, plant maternal 13000-15000 caves.
Growth regulator application process：Reach that pollen mother cell is formed in the main fringe breeding time of about 80% sterile line plant
During the phase, every mu sprays the growth regulator of the above-mentioned formulas of 40-60L in sterile line blade face.If being rained after spraying in two hours, mend
Spray is once.In female parent heading 30%, the sterile line of each cell sprays Activol by the normal production of hybrid seeds and breeding requirement.
Experiment sterile line material used is Toyota 1A, in sterile line Toyota 1A Post flowerings, respectively with restorer Toyota 1B and
Maintainer RC6 combos are hybridized.Control is not in addition to spraying the growth regulator, and remaining experimental condition is identical.Experiment is used
RANDOMIZED BLOCK DESIGN, repeatedly district's groups, the results are shown in Table 3 and table 4 twice.
As a result show：In terms of number of productive ear, in two production of hybrid seeds combination, there is significance difference in the number of productive ear of processing with compareing
It is different：In wherein cross combination Toyota 1A × Toyota 1B, the number of productive ear of processing improves 13.5% than control；It is rich in cross combination
In field 1A × RC6, number of productive ear improves 23.3% compared to control.
In terms of theoretical yield, control is all remarkably higher than with the theoretical yield of the hybrid seeding of Toyota's 1B and RC6 combo：With
The output increased of the hybrid seeding of maintainer Toyota 1B combos 18.3%；With the output increased of the hybrid seeding of RC6 combos
24.5%.
Table 3 handles the influence to combining Toyota 1A × Toyota 1B hybrid seed yield constituent elements
Note：Different lowercase letter indication differences are up to 5% level of signifiance in same row, and same letter represents that difference is not notable.
Table 4 handles the influence to combining Toyota 1A × RC6 hybrid seed yield constituent elements
Note：Different lowercase letter indication differences are up to 5% level of signifiance in same row, and same letter represents that difference is not notable.
Benefit estimation：
Cost estimation：According to Alibaba's net price, 90% purity of Shanghai Vegcides Bio-farm's production
2,4- 340 yuan of epi-brassinolide 500g prices, it is monovalent 0.68 yuan equivalent to every g；Suzhou day can trade Co., Ltd production
100g fills 380 yuan of (98% purity) 3-indolyl acetic acid price, monovalent 3.8 yuan equivalent to every g；The many good fortune Buddhist nun biotechnologies of Nanjing Olympic have
100mg 185 yuan of tissue culture reagent zeatin (purity 99%) prices of dress of limit company production, it is monovalent 1.85 yuan equivalent to every mg.Press
According to average mu consumption 50L meters, every mu is using reagent cost：2,4- epi-brassinolides 0.05mg × 50/1000 × 0.68+3-
Heteroauxin 30mg × 50/1000 × 3.8+ zeatin 1.0mg × 50 × 1.85=0.002+5.7+92.5=98.202 members.
Income estimation：Sterile line breeding, every mu of breeding increases production 19.8kg, and company purchases 40 yuan/kg from peasant household, increases income 792
Member, removes about 98 yuan of reagent cost, and the 2-3 buckets of 50L about conventional nebulizers, 20 yuan of labour cost, peasant household has a net increase of 774 yuan of receipts.It is miscellaneous
The rice production of hybrid seeds is handed over, every mu of volume increase 24.6kg, company increases income 738 yuan, remove 98 yuan of reagent cost and people from peasant household 30 yuan/kg of purchase
20 yuan of expenses of labour, peasant household has a net increase of 620 yuan of receipts.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (9)
1. a kind of growth regulator for improving breeding of hybrid rice and sterile line breeding yield, the growth regulator is included such as
Lower component：2,4- epi-brassinolides, 3-indolyl acetic acid, zeatin and water, pH value 5.8~6.5；
The composition of the growth regulator be 1L water in contain 2,4- epi-brassinolides 0.05-0.1mg, 3-indolyl acetic acid 30mg
With zeatin 0.5-1.0mg.
2. growth regulator according to claim 1, it is characterised in that the composition of the growth regulator is to contain in 1L water
There are 2,4- epi-brassinolides 0.1mg, 3-indolyl acetic acid 30mg and zeatin 0.5mg.
3. growth regulator according to claim 1, it is characterised in that the composition of the growth regulator is to contain in 1L water
There are 2,4- epi-brassinolides 0.05mg, 3-indolyl acetic acid 30mg and zeatin 1.0mg.
4. the preparation method of any one of the claim 1-3 growth regulators, it is characterised in that weigh 2,4- tables oil in proportion
Rape lactone, 3-indolyl acetic acid and zeatin, first dissolve above-mentioned each component with a small amount of 95% alcohol, are then dissolved in water, adjust pH
Value 5.8~6.5, is then settled to 1L.
5. any one of the claim 1-3 growth regulators are in breeding of hybrid rice and sterile line breeding yield is improved
Using.
6. application according to claim 5, it is characterised in that using the method for foliage-spray, in sterile line Secondary branch
Former base and clever flower primordium idiophase, i.e. the ear differentiation third phase, the growth regulator described in claim 3 is sprayed, every mu sprays
40-60L, if rained after spraying in two hours, fills spray once.
7. application according to claim 5, it is characterised in that female thin in sterile line pollen using the method for foliage-spray
Born of the same parents form phase, i.e. the ear differentiation fifth phase, spray the growth regulator described in claim 4, every mu sprays 40-60L, if spray
Rain, then fill spray once in two hours after applying.
8. the application according to claim any one of 5-7, it is characterised in that the field of breeding of hybrid rice and sterile line breeding
Between implantation methods be：The plantation specification of sterile line is 16.5cm × 20cm, and individual plant transplanting, parental is 2:10, remove behaviour
Make aisle, every mu is planted male parent 1500-2000 caves, plants maternal 13000-15000 caves.
9. the application according to claim any one of 5-7, it is characterised in that rice material used in breeding of hybrid rice
Including sterile line Toyota 1A, restorer Toyota 1B, maintainer RC6.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510917621.0A CN105494372B (en) | 2015-12-10 | 2015-12-10 | Growth regulator and the application of breeding of hybrid rice and sterile line breeding yield can be improved |
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| CN201510917621.0A CN105494372B (en) | 2015-12-10 | 2015-12-10 | Growth regulator and the application of breeding of hybrid rice and sterile line breeding yield can be improved |
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| CN105494372B true CN105494372B (en) | 2017-09-15 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108739158A (en) * | 2018-04-26 | 2018-11-06 | 中国水稻研究所 | Application and application process of the brassinosteroid in alleviating rice florescence High Temperature Stress |
| CN108633897B (en) * | 2018-06-12 | 2020-07-14 | 湖南农业大学 | Growth regulator for promoting root growth of ratoon rice and application method thereof |
| CN113080197A (en) * | 2021-03-26 | 2021-07-09 | 浙江大学 | Dehydrating agent for protecting quality of hybrid rice seed production seeds and preparation method and application thereof |
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| GB2481952A (en) * | 2009-04-07 | 2012-01-11 | Taminco Nv | Plant growth regulator additive |
| CN101941872B (en) * | 2010-08-19 | 2013-01-09 | 北京农学院 | Pesticidal foliage fertilizer mixed with plant growth factors and preparation method thereof |
| CN104871955B (en) * | 2015-04-16 | 2017-05-03 | 浙江农林大学 | Method for spraying field exogenous hormone for improving vigor of hybrid rice seeds |
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