CN105494372A - Growth regulating agent capable of improving hybrid rice seed production and sterile line seed reproduction yield and application - Google Patents
Growth regulating agent capable of improving hybrid rice seed production and sterile line seed reproduction yield and application Download PDFInfo
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- CN105494372A CN105494372A CN201510917621.0A CN201510917621A CN105494372A CN 105494372 A CN105494372 A CN 105494372A CN 201510917621 A CN201510917621 A CN 201510917621A CN 105494372 A CN105494372 A CN 105494372A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
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- Genetics & Genomics (AREA)
- Developmental Biology & Embryology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
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Abstract
The invention provides a growth regulating agent capable of improving hybrid rice seed production and sterile line seed reproduction yield. The growth regulating agent is prepared from the following components: 2,4-epibrassinolide, indole-3-acetic acid, zeatin and water, and the pH value is 5.8 to 6.5. By using the growth regulating agent provided by the invention, in hybrid rice seed production and sterile line seed reproduction, the productive tiller percentage of a sterile line can be improved, so as to increase the number of productive ear, floret degeneration can be reduced, so as to increase kernels per spike, grouting is promoted, so as to improve ripening rate and/or increase thousand seed weight, and finally the hybrid rice seed production yield and the sterile line seed reproduction yield are improved. The growth regulating agent is simple and easy in preparation method, is good in product stability, strong in efficiency, safe and reliable, free of side effect and is suitable for popularization and application in hybrid rice seed production.
Description
Technical field
The present invention relates to plant growth regulator technical field, specifically, relate to a kind of growth regulator and the application thereof that improve breeding of hybrid rice and male sterile line breeding output.
Background technology
China is eating rice production state maximum in the world and country of consumption, and the population of more than 60% take rice as staple food.Heterotic utilization significantly improves the per unit area yield of the production of China paddy rice and total product, for ensureing that China's grain security makes a great contribution.
The important channel of rice heterosis utilization is hybrid seeding, but under normal circumstances, the actual production of hybrid seeding and male sterile line breeding is on the low side, greatly governs the development of hybrid rice.
In addition, paddy rice fertilizer requirement in planting process is large, but the traditional fertilising of China often availability is low, and soil to be damaged.Plant growth regulator is used for foliage-spray, and consumption is few, instant effect, and low to the harm of environment, is more suitable for applying widely.
Summary of the invention
The object of this invention is to provide a kind of growth regulator and the application thereof that improve the paddy rice cross breeding production of hybrid seeds and male sterile line breeding output.
In order to realize the object of the invention, a kind of growth regulator improving breeding of hybrid rice and male sterile line breeding output of the present invention, described growth regulator comprises following component: 2,4-epi-brassinolide, 3-indolyl acetic acid, zeatin and water, pH value 5.8 ~ 6.5.
Preferably, consisting of in 1L water of described growth regulator contains 2,4-epi-brassinolide 0.05-0.1mg, 3-indolyl acetic acid 30mg and zeatin 0.5-1.0mg.
More preferably, consisting of in 1L water of described growth regulator (formula I) contains 2,4-epi-brassinolide 0.1mg, 3-indolyl acetic acid 30mg and zeatin 0.5mg.Spray in male sterile line blade face at male sterile line second time branch stalk and clever flower primordium idiophase (the ear differentiation third phase).
More preferably, consisting of in 1L water of described growth regulator (formula I I) contains 2,4-epi-brassinolide 0.05mg, 3-indolyl acetic acid 30mg and zeatin 1.0mg.Spray in male sterile line blade face at male sterile line pollen mother cell formation stage (the ear differentiation fifth phase).
The present invention also provides the preparation method of described growth regulator, take 2 in proportion, 4-epi-brassinolide, 3-indolyl acetic acid and zeatin, first dissolve above-mentioned each component (alcohol contributes to the dissolubility increasing each raw material) with a small amount of 95% alcohol, then soluble in water, adjust pH 5.8 ~ 6.5, is then settled to 1L.
The present invention further provides described growth regulator and improving the application in the paddy rice cross breeding production of hybrid seeds and male sterile line breeding, concrete mode is as follows:
The field planting method of breeding of hybrid rice and male sterile line breeding is: the plantation specification of male sterile line is 16.5cm × 20cm, individual plant transplanting, and parental is 2:10, and removing operation aisle, plants male parent 1500-2000 cave for every mu, plant maternal 13000-15000 cave.Can adjust as required during practical application.
In the former base of male sterile line Secondary branch and clever flower primordium idiophase, i.e. the ear differentiation third phase, spray the growth regulator of formula I, every mu sprays 40-60L, rains, then fill spray once if sprayed in latter two hours.
Adopt the method for foliage-spray, at male sterile line pollen mother cell formation stage, i.e. the ear differentiation fifth phase, spray the growth regulator of formula I I, every mu sprays 40-60L, rains, then fill spray once if sprayed in latter two hours.
Wherein, the rice material that breeding of hybrid rice uses includes but not limited to male sterile line Toyota 1A, restorer Toyota 1B, maintainer RC6.
Growth regulator provided by the invention, in breeding of hybrid rice and male sterile line breeding, the percentage of earbearing tiller of male sterile line can be improved and then increase number of productive ear, reduce floret degeneration and then increase grain number per spike, promote grouting and then improve ripening rate and/or increase thousand kernel weight, finally improving hybrid seed production yields and male sterile line breeding output.Its compound method is simple, and product stability is good, by force both effectiveness, safe and reliable, has no side effect, and is suitable for applying in hybrid rice seeds is produced.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.If do not specialize, the conventional means that technological means used in embodiment is well known to those skilled in the art, is raw materials usedly commercial goods.
Embodiment 1 can improve growth regulator and the application thereof of breeding of hybrid rice and male sterile line breeding output
The formula (content of every 1L) of the present embodiment growth regulator: 2,4-epi-brassinolide 0.1mg, 3-indolyl acetic acid 30mg, zeatin 0.5mg, pH value 5.8 ~ 6.5.
The preparation method of growth regulator: take 2,4-epi-brassinolide, 3-indolyl acetic acid and zeatin in proportion, first dissolve above-mentioned each component with a small amount of 95% alcohol, then soluble in water, adjust pH 5.8 ~ 6.5, is then settled to 1L.
The field planting method (can adjust as required during practical application) of breeding of hybrid rice and breeding: the plantation specification of male sterile line is 16.5cm × 20cm, individual plant transplanting, parental is 2:10, removing operation aisle, plant male parent 1500-2000 cave for every mu, plant maternal 13000-15000 cave.
Growth regulator application process: when the main fringe of the male sterile line plant about 80% reaches second time branch stalk and clever flower primordium idiophase breeding time, every mu of growth regulator spraying the above-mentioned formula of 40-60L is in male sterile line blade face.Rain if spray in latter two hours, then fill spray once.Female parent ear 30% time, the male sterile line of each community sprays Activol (gibberellin) by the normal production of hybrid seeds and breeding requirement.
Testing sterile line material used is Toyota 1A, at male sterile line Toyota 1A Post flowering, hybridizes respectively by restorer Toyota 1B and maintainer RC6 combo.Contrast is not except spraying this growth regulator, and all the other experimental conditions are identical.Test adopts randomised block design, and twice duplicate block group, the results are shown in Table 1 and table 2.
Result shows: number of productive ear aspect, and in hybrid combination Toyota 1A × Toyota 1B, the number of productive ear of process exists significant difference with contrasting, and the number of productive ear wherein processed improves 12.6% than contrast.In hybrid combination Toyota 1A × RC6, there is not significant difference with contrasting in number of productive ear.Every total grain panicle number aspect, process and there is significant difference with contrasting, wherein, every total grain panicle number of process improves 11.4% than contrast.
In thousand kernel weight, in hybrid combination Toyota 1A × Toyota 1B, process with the thousand kernel weight contrasted without marked difference.In hybrid combination Toyota 1A × RC6, thousand kernel weight compares according to improve 7.3%, and difference reaches remarkable.
In theoretical yield, the hybrid seed yield of hybrid combination Toyota 1A × Toyota 1B and combination Toyota 1A × RC6 is all high than contrast, and reaches significant difference.Wherein, the hybrid seed yield of hybrid combination Toyota 1A × Toyota 1B is higher by 22.7% than contrast, and the hybrid seed yield of combination Toyota 1A × RC6 is higher by 34.1% than contrast.
Table 1 processes the impact on combination Toyota 1A × Toyota 1B breeding yield component
Note: in same row, different lowercase alphabet differential is different reaches 5% significance level, and same letter represents that difference is not remarkable.
Table 2 processes the impact on combination Toyota 1A × RC6 hybrid seed yield constituent element
Note: in same row, different lowercase alphabet differential is different reaches 5% significance level, and same letter represents that difference is not remarkable.
Benefit estimation:
Cost estimation: according to Alibaba's net price, 90% purity 2, the 4-epi-brassinolide 500g price 340 yuan that Shanghai Vegcides Bio-farm (Nanchang) Co., Ltd. produces, is equivalent to every g unit price 0.68 yuan; Sky, Suzhou can trade Co., Ltd produce 100g fill (98% purity) 3-indolyl acetic acid price 380 yuan, be equivalent to every g unit price 3.8 yuan; 100mg dress group training reagent zeatin (purity 99%) price 185 yuan that Nanjing Olympic Duo Fu Buddhist nun bio tech ltd produces, is equivalent to every mg unit price 1.85 yuan.Count according to average mu consumption 50L, every mu of use reagent cost is: 2,4-epi-brassinolide 0.1mg × 50/1000 × 0.68+3-heteroauxin 30mg × 50/1000 × 3.8+ zeatin 0.5mg × 50 × 1.85=0.003+5.7+46.25=51.753 unit.
Income estimation: male sterile line breeding, every mu of breeding volume increase 23.8kg, 40 yuan/kg purchases from peasant household in company, increases income 952 yuan, and removing reagent cost about 52 yuan, the 2-3 bucket of the about conventional nebulizers of 50L, labour cost 20 yuan, peasant household has a net increase of receipts 880 yuan.The hybrid rice production of hybrid seeds, every mu of volume increase 34.2kg, 30 yuan/kg purchases from peasant household in company, increases income 1026 yuan, and removing reagent cost 52 months and labour cost 20 yuan, peasant household has a net increase of receipts 954 yuan.
Embodiment 2 can improve growth regulator and the application thereof of breeding of hybrid rice and male sterile line breeding output
The formula (content of every 1L) of the present embodiment growth regulator: containing 2,4-epi-brassinolide 0.05mg, 3-indolyl acetic acid 30mg, zeatin 1.0mg in 1L water, pH value 5.8 ~ 6.5.
The preparation method of growth regulator: take 2,4-epi-brassinolide, 3-indolyl acetic acid and zeatin in proportion, first dissolve above-mentioned each component with a small amount of 95% alcohol, then soluble in water, adjust pH 5.8 ~ 6.5, is then settled to 1L.
The field planting method (can adjust as required during practical application) of breeding of hybrid rice and breeding: the plantation specification of male sterile line is 16.5cm × 20cm, individual plant transplanting, parental is 2:10, removing operation aisle, plant male parent 1500-2000 cave for every mu, plant maternal 13000-15000 cave.
Growth regulator application process: when the main fringe of the male sterile line plant about 80% reaches pollen mother cell formation stage breeding time, every mu of growth regulator spraying the above-mentioned formula of 40-60L is in male sterile line blade face.Rain if spray in latter two hours, then fill spray once.Female parent ear 30% time, the male sterile line of each community sprays Activol by the normal production of hybrid seeds and breeding requirement.
Testing sterile line material used is Toyota 1A, at male sterile line Toyota 1A Post flowering, hybridizes respectively by restorer Toyota 1B and maintainer RC6 combo.Contrast is not except spraying this growth regulator, and all the other experimental conditions are identical.Test adopts randomised block design, and twice duplicate block group, the results are shown in Table 3 and table 4.
Result shows: number of productive ear aspect, and in two production of hybrid seeds combinations, the number of productive ear of process all exists significant difference with contrasting: wherein in hybrid combination Toyota 1A × Toyota 1B, the number of productive ear of process improves 13.5% than contrast; In hybrid combination Toyota 1A × RC6, number of productive ear compares according to improve 23.3%.
In theoretical yield, be all significantly higher than contrast with the theoretical yield of the hybrid seeding of Toyota 1B and RC6 combo: with the output increased of the hybrid seeding of maintainer Toyota 1B combo 18.3%; With the output increased of the hybrid seeding of RC6 combo 24.5%.
Table 3 processes the impact on combination Toyota 1A × Toyota 1B hybrid seed yield constituent element
Note: in same row, different lowercase alphabet differential is different reaches 5% significance level, and same letter represents that difference is not remarkable.
Table 4 processes the impact on combination Toyota 1A × RC6 hybrid seed yield constituent element
Note: in same row, different lowercase alphabet differential is different reaches 5% significance level, and same letter represents that difference is not remarkable.
Benefit estimation:
Cost estimation: according to Alibaba's net price, 90% purity 2, the 4-epi-brassinolide 500g price 340 yuan that Shanghai Vegcides Bio-farm (Nanchang) Co., Ltd. produces, is equivalent to every g unit price 0.68 yuan; Sky, Suzhou can trade Co., Ltd produce 100g fill (98% purity) 3-indolyl acetic acid price 380 yuan, be equivalent to every g unit price 3.8 yuan; 100mg dress group training reagent zeatin (purity 99%) price 185 yuan that Nanjing Olympic Duo Fu Buddhist nun bio tech ltd produces, is equivalent to every mg unit price 1.85 yuan.Count according to average mu consumption 50L, every mu of use reagent cost is: 2,4-epi-brassinolide 0.05mg × 50/1000 × 0.68+3-heteroauxin 30mg × 50/1000 × 3.8+ zeatin 1.0mg × 50 × 1.85=0.002+5.7+92.5=98.202 unit.
Income estimation: male sterile line breeding, every mu of breeding volume increase 19.8kg, 40 yuan/kg purchases from peasant household in company, increases income 792 yuan, and removing reagent cost about 98 yuan, the 2-3 bucket of the about conventional nebulizers of 50L, labour cost 20 yuan, peasant household has a net increase of receipts 774 yuan.The hybrid rice production of hybrid seeds, every mu of volume increase 24.6kg, 30 yuan/kg purchases from peasant household in company, increases income 738 yuan, and removing reagent cost 98 yuan and labour cost 20 yuan, peasant household has a net increase of receipts 620 yuan.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
1. can improve a growth regulator for breeding of hybrid rice and male sterile line breeding output, described growth regulator comprises following component: 2,4-epi-brassinolide, 3-indolyl acetic acid, zeatin and water, pH value 5.8 ~ 6.5.
2. growth regulator according to claim 1, is characterized in that, consisting of in 1L water containing 2,4-epi-brassinolide 0.05-0.1mg, 3-indolyl acetic acid 30mg and zeatin 0.5-1.0mg of described growth regulator.
3. growth regulator according to claim 2, is characterized in that, consisting of in 1L water containing 2,4-epi-brassinolide 0.1mg, 3-indolyl acetic acid 30mg and zeatin 0.5mg of described growth regulator.
4. growth regulator according to claim 2, is characterized in that, consisting of in 1L water containing 2,4-epi-brassinolide 0.05mg, 3-indolyl acetic acid 30mg and zeatin 1.0mg of described growth regulator.
5. the preparation method of growth regulator described in any one of claim 1-4, is characterized in that, take 2 in proportion, 4-epi-brassinolide, 3-indolyl acetic acid and zeatin, first dissolve above-mentioned each component with a small amount of 95% alcohol, then soluble in water, adjust pH 5.8 ~ 6.5, is then settled to 1L.
6. growth regulating described in any one of claim 1-4 is multiple is improving the application in breeding of hybrid rice and male sterile line breeding output.
7. application according to claim 6, it is characterized in that, adopt the method for foliage-spray, in the former base of male sterile line Secondary branch and clever flower primordium idiophase, the i.e. ear differentiation third phase, spray growth regulator according to claim 3, every mu sprays 40-60L, rain if sprayed in latter two hours, then fill spray once.
8. application according to claim 6, is characterized in that, adopts the method for foliage-spray, at male sterile line pollen mother cell formation stage, i.e. the ear differentiation fifth phase, spray growth regulator according to claim 4, every mu sprays 40-60L, rains, then fill spray once if sprayed in latter two hours.
9. the application according to any one of claim 6-8, it is characterized in that, the field planting method of breeding of hybrid rice and male sterile line breeding is: the plantation specification of male sterile line is 16.5cm × 20cm, individual plant transplanting, parental is 2:10, removing operation aisle, plants male parent 1500-2000 cave for every mu, plants maternal 13000-15000 cave.
10. the application according to any one of claim 6-8, is characterized in that, the rice material that breeding of hybrid rice uses comprises male sterile line Toyota 1A, restorer Toyota 1B, maintainer RC6.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108633897A (en) * | 2018-06-12 | 2018-10-12 | 湖南农业大学 | A kind of growth regulator and its method of administration of the root growth of promotion ratooning rice |
| CN108739158A (en) * | 2018-04-26 | 2018-11-06 | 中国水稻研究所 | Application and application process of the brassinosteroid in alleviating rice florescence High Temperature Stress |
| CN113080197A (en) * | 2021-03-26 | 2021-07-09 | 浙江大学 | Dehydrating agent for protecting quality of hybrid rice seed production seeds and preparation method and application thereof |
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2015
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| CN101941872B (en) * | 2010-08-19 | 2013-01-09 | 北京农学院 | Pesticidal foliage fertilizer mixed with plant growth factors and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108739158A (en) * | 2018-04-26 | 2018-11-06 | 中国水稻研究所 | Application and application process of the brassinosteroid in alleviating rice florescence High Temperature Stress |
| CN108633897A (en) * | 2018-06-12 | 2018-10-12 | 湖南农业大学 | A kind of growth regulator and its method of administration of the root growth of promotion ratooning rice |
| CN108633897B (en) * | 2018-06-12 | 2020-07-14 | 湖南农业大学 | Growth regulator for promoting root growth of ratoon rice and application method thereof |
| CN113080197A (en) * | 2021-03-26 | 2021-07-09 | 浙江大学 | Dehydrating agent for protecting quality of hybrid rice seed production seeds and preparation method and application thereof |
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| CN105494372B (en) | 2017-09-15 |
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