CN1843087A - High stigma exposure rate japonica rice sterile line selection and breeding technology - Google Patents

Abstract

The invention relates to a method for breeding a sterile round-shaped rice with exposed high stigma, comprising the following steps: (1) crossing round-shaped rice with Shan rice and getting F1; (2) backcrossing the F1 with round-shaped rice to get BC1F1; (3) getting BC1F2 through BC1F1 with high exposed stigma rate auto-crossing and getting BC2F1 through backcrossing with round-shaped rice; (4) planting the BC1F1 in large group for two generations, auto-crossing and collecting together, getting BC3F1; (6) planting BC2F2 in large group, autocrossing and collecting together, getting BC2F3; (7) BC3F1 autocrossing, collecting together and getting BC3F2; (8) planting BC1F4, BC2F3 and BC3F2 in large group respectively, chossing strains and breeding sterile strain; (9) getting stable sterile strain through successive backcross with sterile strain for 4-5 generations. The invention can effectively increase the exposed stigma rate of round-shaped rice from 10-20% to above 60%, and thus increase the fruit-producing rate of the hybrid and increase the productivity.

Description

The breeding technique of high stigma exposure rate japonica rice sterile line

(1) technical field

The breeding technique that the present invention relates to the Japonica Hybrid male sterile line is the breeding technique that improves the high different friendship characteristic of japonica rice sterile line.

(2) background technology

Paddy rice is an important crops, is most people's staple food in the world.The raising of rice yield has great importance for guarantor's group food safety.Hybrid rice generally has the volume increase advantage of 15-20%, plays an important role in increasing production of rice, compares with the hybridization long-grained nonglutinous rice, and Japonica Hybrid is because different friendship difference and hybrid seed yield is low, and its development slowly relatively always.The height of the stigma exposing ratio of male sterile line is one of main direct factor that influences its production of hybrid seeds outcrossing.High stigma exposure rate is the inherent characteristic of long-grained nonglutinous rice, and the stigma exposing ratio of indica cms line is usually up to more than 80%, and hybrid seed yield is generally at 4.5t/ha; And japonica rice; Column cap is little and exposing ratio is low, the stigma exposing ratio of general japonica rice sterile line is below 20%, hybrid seed yield only is 1.5t/ha: therefore cannot improve the stigma exposing ratio of japonica rice sterile line, will improve the different friendship of japonica rice sterile line effectively, improve hybrid seeding output: promote the development of Japonica Hybrid.

Show from the interrelated data retrieval that comprises Chinese patent, have not yet to see the breeding technique of being high stigma exposure rate japonica rice sterile line.

(3) summary of the invention

In order to solve the low problem of japonica rice sterile line stigma exposing ratio, the invention provides the method that a kind of high stigma exposure rate gene with long-grained nonglutinous rice imports japonica rice, the seed selection high stigma exposure rate japonica rice sterile line.

The technical scheme that adopts:

The breeding technique of high stigma exposure rate japonica rice sterile line, its feature comprises following process:

1) japonica rice and indica hybrid get F1;

2) F1 generation and japonica rice backcross BC1F1;

3) select the high further selfing of BC1F1 of stigma exposing ratio and backcross with japonica rice and to obtain BC1F2 and BC2F1 respectively;

4) BC1F2 carries out the large group plantation of two generations, and selfing and group mix and receive, and finally obtain BC1F4;

5) select the high plant of stigma appearing to continue selfing and backcross to obtain BC2F2 and BC3F1 respectively from BC2F1 with japonica rice;

6) BC2F2 colony plantation, selfing and group mix receive BC2F3;

7) BC3F1 selfing group mix receive BC3F2;

8) BC1F4, BC2F3 and BC3F2 carry out colony's plantation respectively, are choosing and transformation male sterile line;

9) process 4-5 is for becoming stable sterile line with the continuous backcross transformation of male sterile line.

Major advantage of the present invention:

The present invention adopts the method that the high stigma exposure rate gene of long-grained nonglutinous rice is imported japonica rice, seed selection high stigma exposure rate japonica rice type rice sterile line.This invention can improve the stigma exposing ratio of Japonica rice male sterile line effectively, is brought up to more than 60% by original 10-20%, closely improves the outcrossing seed-setting rate of hybrid seeding, improves hybrid seed yield.

(4) description of drawings

Fig. 1 is an operational flowchart of the present invention.

(5) embodiment

Embodiment one

The seed selection of the 30A of Japonica rice male sterile line the Liao Dynasty: be the advanced generation cross of reestablishing diplomatic relations that utilizes high stigma exposure rate material B 9 and japonica rice maintenance line material LS2/ pearl round-grained rice // the Liao Dynasty 294, the continuous selfing of plant of selection high stigma exposure rate is selected and is backcrossed with the Liao Dynasty 30 from filial generation, will the high stigma exposure rate gene import through 6 generations and to breed the 30B of round-grained rice type maintenance line the Liao Dynasty in the Liao Dynasty 30, the 30B of the Liao Dynasty is near and transformation becomes the 30A of BT type cytoplasmic male sterile line the Liao Dynasty, opens clever stigma exposing ratio up to 80%.

Embodiment two

The seed selection of the 105A of japonica rice sterile line the Liao Dynasty: progeny material rotational crossing and the continuous selfing of adopting high stigma exposure rate material B 9 and LS2/ pearl round-grained rice // 8958 to reestablish diplomatic relations are selected, breed the 105B of high stigma exposure rate maintenance line the Liao Dynasty through 7 generations, the 105B of the Liao Dynasty is near and transformation becomes the 105A of male sterile line the Liao Dynasty, opens clever stigma exposing ratio and reaches 75%.

Embodiment three

The 02A of japonica rice sterile line the Liao Dynasty also adopts this method seed selection to form, be with the high stigma exposure rate material agricultural precious B in south and No. 2 continuous backcrosses of pearl round-grained rice and selfing selection, breed the 02B of high stigma exposure rate maintenance line the Liao Dynasty through 6 generations, the 1992-1995 transformation becomes the stable 02A of male sterile line the Liao Dynasty, opens clever stigma exposing ratio and reaches 60%.

Claims (1)

1, the breeding technique of high stigma exposure rate japonica rice sterile line, its feature comprises following process:

(1) japonica rice and indica hybrid get F1;

(2) F1 generation and japonica rice backcross BC1F1;

(3) select the high further selfing of BC1F1 of stigma exposing ratio and backcross with japonica rice and to obtain BC1F2 and BC2F1 respectively;

(4) BC1F2 carries out the large group plantation of two generations 1000 strains, and selfing and group mix and receive, and finally obtain BC1F4;

(5) select the high plant of stigma appearing to continue selfing and backcross to obtain BC2F2 and BC3F respectively from BC2FI with japonica rice;

(6) the BC2F2 kind is planted 500 strains, selfing and group mix receive BC2F3;

(7) BC3F1 selfing group mix receive BC3F2;

(8) BC1F4, BC2F3 and BC3F2 plant the colony of 1000 strains, 500 strains and 300 strains respectively and select also transformation male sterile line;

(9) process 4-5 is for becoming stable sterile line with the continuous backcross transformation of male sterile line.

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