CN114009333B

CN114009333B - Method for improving seed production yield by hybridizing maize DH sister lines

Info

Publication number: CN114009333B
Application number: CN202111431981.1A
Authority: CN
Inventors: 番兴明; 蒋辅燕; 尹兴福; 姚文华; 陈洪梅
Original assignee: Food Crops Research Institute yunnan Academy Of Agricultural Sciences
Current assignee: Food Crops Research Institute yunnan Academy Of Agricultural Sciences
Priority date: 2021-11-29
Filing date: 2021-11-29
Publication date: 2023-04-11
Anticipated expiration: 2041-11-29
Also published as: CN114009333A

Abstract

The method for improving the seed production yield by using the corn DH sister line hybridization comprises the following steps: a female parent of the corn hybrid is induced to double by using haploid technology to obtain a plurality of female parent DH sister lines with similar genetic backgrounds and one hundred percent of homozygous genotypes. Because the genetic background of each DH sister line is similar, the hybrid seeds generated by the hybridization with the original corn hybrid male parent have better consistency on the post-representative type, and the original variety attribute is not changed. Meanwhile, the DH sister lines have certain heterosis due to the difference and interaction of individual loci, and the yield of the F1 generation of the hybridization of the two DH sister lines is greatly improved compared with the yield of the original female parent selfing line. The invention utilizes the hybrid F1 generation seeds of the DH sister line to replace the original commercial maize hybrid female parent seeds for producing and producing the seeds, improves the seed production yield by 40 percent at most on the premise of not changing the variety attribute, greatly reduces the seed production cost and increases the market competitiveness of the variety.

Description

Method for improving seed production yield by using corn DH sister line hybridization

Technical Field

The invention relates to the technical field of maize DH breeding and maize seed production, in particular to a method for improving seed production yield by utilizing maize DH sister line hybridization.

Background

Corn is the grain crop with the largest planting area and yield in China, and plays a decisive role in national grain and livestock production safety. Corn is one of the crops which utilize heterosis at the earliest and is also the grain crop which utilizes heterosis most successfully and is widely applied. More than 90% of maize varieties popularized and planted in the current market are hybrid varieties produced by hybridizing 2 genotype homozygous inbred lines. The traditional maize inbred line breeding generally needs continuous inbred for more than 7-8 generations to form a line with relatively homozygous genotype. As for tropical and subtropical maize germplasm, due to abundant and diverse genetic variation, a selfing line with very homozygous genotype is difficult to obtain by the traditional selfing breeding method. Meanwhile, the germplasm of the tropical and subtropical maize contains abundant genetic variation and excellent characteristics: such as developed root system, long green-keeping period, strong stress resistance, strong disease and pest resistance, high yield, excellent grain quality and the like, and is a core germplasm resource for cultivating high-yield and high-resistance corn breakthrough new varieties.

The corn haploid doubling technology is an important tool for breeding a corn inbred line, induced materials are used as female parents, a corn haploid induction line is used as a male parent for hybridization, haploid grains are selected from progeny for planting and doubling, and the corn DH line with homozygous genotype can be produced through inbreeding. The utilization of the technology can greatly shorten the breeding period of the corn and improve the breeding efficiency. However, because of genotype homozygosis, no heterosis exists, the maize inbred line has short ears, low seed production yield and high seed production cost. Therefore, the technical problem to be solved by the technical personnel in the field is to provide a method for improving seed production yield by utilizing the corn DH sister line hybridization.

Disclosure of Invention

The commercial maize hybrid parent inbred lines popularized and applied in large area in production all have certain heterozygosity, especially the genetic backgrounds of tropical and subtropical maize germplasm are complex and diverse, the parent inbred lines bred by using the traditional breeding method are difficult to realize one hundred percent of homozygous genotype, and the yield is relatively low. In view of the above, the invention provides a method for improving seed production yield by hybridizing maize DH sister lines.

In order to achieve the purpose, the invention adopts the following technical scheme:

the method for improving seed production yield by utilizing corn DH sister line hybridization comprises the following steps:

(1) Taking a female parent of a corn hybrid as an induced material and a corn haploid induction line as a male parent to perform hybridization to obtain haploid grains;

(2) The obtained haploid grains are subjected to seedling raising, transplanting and planting in the field for natural doubling to obtain successfully doubled corn plants;

(3) Bagging the plants successfully doubled for selfing and fructification to respectively obtain a plurality of corn DH sister lines from the female parent;

(4) Hybridizing the obtained DH sister lines to obtain F1 generation seeds with greatly improved yield;

(5) F1 generation seeds obtained by respective DH sister line hybridization are respectively used for replacing female parents to carry out seed production, and the seed production yield is improved.

Furthermore, the female parent of the corn hybrid is the female parent of a commercial hybrid with certain heterozygosity.

Further, the female parent materials in the step (1) are a female parent self-bred line (DHR 146) of the corn hybrid Yunrei 408 and a female parent self-bred line (CML 171-1-1-2-1-1) of the corn hybrid Yun Rui.

The invention has the beneficial effects that: because the genetic background of each DH sister line is similar, the hybrid seeds generated by hybridization with the original commercial corn hybrid male parent have better consistency on the later representative type, and the original variety attribute is not changed. Meanwhile, because of the difference and interaction of individual sites between the DH sister lines, a certain heterosis is achieved, and the yield of the hybrid F1 generation of the two DH sister lines is greatly improved compared with that of the original female parent inbred line. The method utilizes the hybrid F1 generation seeds of the DH sister line to replace the original commercial corn hybrid female parent seeds for seed production, improves the seed production yield by 40 percent to the maximum on the premise of not changing the variety attribute, greatly reduces the seed production cost and increases the market competitiveness of the variety.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Examples

The method for improving the seed production yield by using the corn DH sister line hybridization comprises the following steps:

(1) Respectively taking a female parent inbred line (DHR 146) of the corn hybrid Yunrui 408 and a female parent inbred line (CML 171-1-1-2-1-1) of the corn hybrid Yun Rui No. 538 as induced materials, and taking the corn induction line as a male parent to perform hybridization to respectively obtain haploid grains;

(2) Respectively carrying out seedling raising, transplanting and planting on the obtained haploid grains in a field for natural doubling to obtain successfully doubled corn plants;

(3) Bagging the plants successfully doubled, selfing and maturing to respectively obtain a plurality of corn DH sister lines from DHR146 and CML171-1-1-2-1-1;

(4) Hybridizing the obtained DH sibling lines with the same sources respectively to obtain F1 generation seeds with greatly improved yield;

(5) F1 generation seeds obtained by respective DH sister line hybridization are used for replacing DHR146 and CML171-1-1-2-1-1 to carry out seed production, and the seed production yield of Yun Rui 408 and Yun Rui is greatly improved.

The test was carried out in the city of Jinghong city, yunnan province, in 2020 winter according to the above examples, and the test results are shown in Table 1:

TABLE 1

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As can be seen from the table 1, the method of the invention effectively improves the seed production yield by using the corn DH sister line hybridization, and the maximum improvement amount can reach more than 40 percent.

Although embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are exemplary and not to be construed as limiting the present invention, and that changes, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

Claims

1. The method for improving the seed production yield by using the corn DH sister line hybridization is characterized by comprising the following steps:

(1) Hybridizing a female parent of a corn hybrid seed serving as an induced material and a corn haploid induction line serving as a male parent to obtain haploid grains; the female parent is a female parent inbred line CML171-1-1-2-1-1 of a corn hybrid No. Yun Rui;