CN102657077B - Long-grain hybrid japonica rice restorer selection method - Google Patents
Long-grain hybrid japonica rice restorer selection method Download PDFInfo
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- CN102657077B CN102657077B CN201210152908.5A CN201210152908A CN102657077B CN 102657077 B CN102657077 B CN 102657077B CN 201210152908 A CN201210152908 A CN 201210152908A CN 102657077 B CN102657077 B CN 102657077B
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- 240000008467 Oryza sativa Japonica Group Species 0.000 title claims abstract description 67
- 238000010187 selection method Methods 0.000 title abstract 3
- 235000013339 cereals Nutrition 0.000 claims abstract description 115
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 50
- 235000009566 rice Nutrition 0.000 claims abstract description 50
- 239000000463 material Substances 0.000 claims abstract description 39
- 241000196324 Embryophyta Species 0.000 claims abstract description 18
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 18
- 238000000926 separation method Methods 0.000 claims abstract description 4
- 240000007594 Oryza sativa Species 0.000 claims description 47
- 244000184734 Pyrus japonica Species 0.000 claims description 26
- 238000011084 recovery Methods 0.000 claims description 9
- 241000746966 Zizania Species 0.000 claims description 7
- 235000002636 Zizania aquatica Nutrition 0.000 claims description 7
- 238000009396 hybridization Methods 0.000 claims description 7
- 230000009286 beneficial effect Effects 0.000 claims description 5
- 230000035558 fertility Effects 0.000 claims description 4
- 206010000210 abortion Diseases 0.000 claims description 3
- 231100000176 abortion Toxicity 0.000 claims description 3
- 238000006116 polymerization reaction Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 241000209094 Oryza Species 0.000 abstract 3
- 230000009418 agronomic effect Effects 0.000 abstract 1
- 238000003306 harvesting Methods 0.000 abstract 1
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000005204 segregation Methods 0.000 description 2
- 240000002582 Oryza sativa Indica Group Species 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012214 genetic breeding Methods 0.000 description 1
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Abstract
The invention discloses a long-grain hybrid japonica rice restorer selection method comprising the following steps of: firstly, selecting long-grain rice resource materials as donor parents of grain-type trait genes; secondly, hybridizing the donor parents carrying the long-grain genes with the conventional round-grain japonica rice restorers; thirdly, initially selecting single plants with the rice grain length being more than 6.5mm to harvest seeds in separation of hybrid progenies; and fourthly, performing test-cross on the long-grain japonica rice materials obtained from the step 3 and the sterile lines of japonica rice to test the restoration of the long-grain japonica rice materials, and selecting strains with good restorability and excellent agronomic traits. The selection method is applicable to perform a grain type improvement on the conventional round-grain japonica rice restorer to create the long-grain hybrid japonica rice, can change the conventional round grains of 5.0mm to 5.5mm to the long grains more than or equal to 6.5mm, and remarkably improves the appearance and quality of the rice.
Description
Technical field
The present invention relates to Japonica Hybrid genetic breeding field, particularly, relate to long grain type Japonica Hybrid restorer selection.
Background technology
Asian Cultivated Rice has two subspecies of long-grained nonglutinous rice japonica rice, and Typical Japonica majority is short grain round shaped grain (rice grain length 5.0-5.5mm), has a small amount of long grain type japonica rice resource, but there is no recovery capability.Up to now, Japonica Hybrid Rice Breeding is produced its recovery gene source of Restoring Line of Japonica Rice of upper application in long-grained nonglutinous rice, be short grain round shaped grain (rice grain length 5.0-5.5mm) type, make current Japonica Hybrid not possess long grain appearance and the quality that welcome in market, affect to a certain extent the production development of Japonica Hybrid.Under this background, invent a kind of selection of growing a Restoring Line of Japonica Rice, can significantly improve outward appearance and the market quality of existing Japonica Hybrid restorer, the long grain of initiative type Japonica Hybrid.
Summary of the invention
The object of the present invention is to provide a kind of selection for Japonica Hybrid restorer of growing, it can improve outward appearance and the market quality of existing Restoring Line of Japonica Rice, the long grain of a seed selection type Japonica Hybrid kind, and the production of hybrid seeds of using it for Japonica Hybrid is produced.
To achieve these goals, the present invention adopts following technical scheme:
A kind of long grain Japonica Hybrid restorer selection, comprises the following steps:
(1) select long grain type paddy rice resource material, as the donor parents of grain type character gene;
(2) donor parents and the hybridization of traditional round shaped grain type Restoring Line of Japonica Rice of long grain type gene will be carried;
(3) separate in offspring individual plant results seed more than initial option rice grain length >=6.5mm in hybridization;
(4) with the long grain japonica rice material and the japonica rice sterile test cross that obtain in step (3), measure that it is restorative, select the strain that wherein recovery capability is good, economical character is good.
Further, the long grain type paddy rice resource material in described step (1) is japonica rice, long-grained nonglutinous rice, java rice or the wild rice material of long grain.
Further, the grain length >=6.5mm of the long grain type paddy rice resource material in described step (1).
Further, the traditional round shaped grain type Restoring Line of Japonica Rice in described step (2) is traditional round shaped grain type Restoring Line of Japonica Rice of rice grain length 5.0-5.5mm; Or there is part recovery capability, and on output, quality, resistance, economical character, there is advantage, traditional round shaped grain japonica rice material of rice grain length 5.0-5.5mm.
Further, in described step (3), the long grain japonica rice material obtaining through initial option can backcross 1-2 time with the donor parents of carrying long grain type gene again, separates in offspring at it, further selects individual plant results seed more than rice grain length >=6.5mm.
Further, in described step (3), the long grain japonica rice material obtaining through initial option can be reestablished diplomatic relations or reestablish diplomatic relations and backcross with the material with other excellent proterties again, make other beneficial genes and long kernel Characters polymerization, then separate offspring and further select individual plant more than rice grain length >=6.5mm to gather in the crops seed from it.
The material further, with other excellent proterties is japonica rice, long-grained nonglutinous rice, java rice or wild rice material.
Further, described separation offspring is F2-F5 generation.
Further, the japonica rice sterile in described step (4) is Yunnan I type, BT type, seal water type and/or wild abortion type.
Further, repeating step (4) is further verified its restorative and economical character, obtains the good long grain Restoring Line of Japonica Rice of economical character.
The present invention, owing to adopting technique scheme, has following beneficial effect:
The present invention is by selecting suitable breeding material as parent, adopt hybridize, reestablish diplomatic relations, backcross or long the Japonica Hybrid restorer of multiple breeding method seed selection such as its combination, then verifies exactly its recovery capability by test cross.Selection of the present invention is applicable to existing Restoring Line of Japonica Rice to carry out the improvement of type, the long grain of initiative type Japonica Hybrid, and the round shaped grain type that changes traditional 5.0-5.5mm is >=the long grain type of 6.5mm, significantly improves rice outward appearance and quality.。
Embodiment
A kind of long grain Japonica Hybrid restorer selection of the present invention, comprises the following steps:
(1) select long grain type paddy rice resource material, as the donor parents of grain type character gene;
(2) donor parents and the traditional round shaped grain Restoring Line of Japonica Rice that carry long grain type gene are hybridized, backcross, reestablished diplomatic relations, make long grain type gene, recover gene and the restructuring of other beneficial genes;
(3), at the separation Offspring F2-F5 of several Crossing systems, select individual plant results seed more than rice grain length >=6.5mm;
(4) with the long grain japonica rice material and the japonica rice sterile test cross that obtain in step (3), measure that it is restorative, select the strain that wherein recovery capability is good, economical character is good;
(5) repeating step (4) is verified its restorative and economical character, obtains the good long grain Restoring Line of Japonica Rice of economical character.
Long grain type paddy rice resource material in above-mentioned steps (1) is japonica rice, long-grained nonglutinous rice, java rice or the wild rice material of long grain;
Traditional round shaped grain Restoring Line of Japonica Rice in above-mentioned steps (2) is traditional round shaped grain type Restoring Line of Japonica Rice of rice grain length 5.0-5.5mm; Or there is part recovery capability, and on output, quality, resistance, economical character, there is traditional round shaped grain japonica rice material of advantage, rice grain length 5.0-5.5mm.
The restorer method that obtains seed grain length >=6.5mm in above-mentioned steps (4) has and has two kinds at least: one for selecting to have the individual plant of long kernel Characters in once hybridizing segregation population, require to select in individual plant more than rice grain length >=6.5mm, and then select plant type and the good individual plant of economical character, obtain the long grain Restoring Line of Japonica Rice of artificial modified; Two reestablish diplomatic relations and/or backcross with the material with other excellent proterties for the long grain japonica rice material that primary election is obtained, and make other beneficial genes and long kernel Characters polymerization, obtain the long grain Restoring Line of Japonica Rice of artificial modified.Wherein, the material that has other excellent proterties is japonica rice, long-grained nonglutinous rice, java rice or wild rice material.
Japonica rice sterile in above-mentioned steps (4), comprises Yunnan I type, BT type, prints water type and/or wild abortion type;
The long grain Restoring Line of Japonica Rice obtaining in above-mentioned steps (4), (5), rice grain length >=6.5mm, can be used for preparing hybrid japonica rice new varieties, and the production of hybrid seeds that also can be directly used in Japonica Hybrid combination is produced.
Below by being further elaborated for example:
Embodiment 1
The seed selection of long grain Restoring Line of Japonica Rice L81: select Restoring Line of Japonica Rice C418 and long grain japonica rice material Jiahe 212 to hybridize, select at the from generation to generation long kernel Characters individual plant of filial generation F2-F5, with BT type male sterile line Zhejiang round-grained rice 2A, Yunnan I type male sterile line Chunjiang 12A test cross, in test cross F1, choose that high, restorative good, the long grain type of Spikelet fertility (rice grain length 6.6mm), economical character are good, a long grain Restoring Line of Japonica Rice L81 for blast resisting.
Embodiment 2
The seed selection of long grain Restoring Line of Japonica Rice L42: select Restoring Line of Japonica Rice C418 and long grain japonica rice material Jiahe 212 to hybridize, hybridization F2 segregative generation select the good individual plant of the grain elongated economical character of type again with long grain type germ plasm resource CN109 hybridization, select at the from generation to generation long kernel Characters individual plant of the F2-F5 that reestablishes diplomatic relations, with BT type male sterile line Zhejiang round-grained rice 2A, Yunnan I type male sterile line Chunjiang 12A test cross, in test cross F1, choose that high, restorative good, the long grain type of Spikelet fertility (rice grain length 7.2mm), economical character are good, a long grain Restoring Line of Japonica Rice L42 for blast resisting.
Embodiment 3
The seed selection of long grain Restoring Line of Japonica Rice L917: select Restoring Line of Japonica Rice C418 and long grain japonica rice material Jiahe 212 to hybridize, select the good individual plant of the grain elongated economical character of type to hybridize with Indica Rice Restorer Lines R838 again at hybridization F2 segregative generation, reestablishing diplomatic relations, the further good individual plant of the grain elongated economical character of type of selecting in F2 segregation population backcrosses with parent Jiahe 212 again, select at the from generation to generation long kernel Characters individual plant of the F2-F5 that reestablishes diplomatic relations, with BT type male sterile line Zhejiang round-grained rice 2A, Yunnan I type male sterile line Chunjiang 12A test cross, in test cross F1, choose Spikelet fertility high, restorative good, long grain type (rice grain length 6.9mm), the long grain Restoring Line of Japonica Rice L917 that economical character is good.
The long Restoring Line of Japonica Rice of table 1 and traditional restorer grain type and yield traits comparison
Above-described embodiment 1-3 adopts long grain type japonica rice variety as donor parents, in like manner, can also select to have long grain type gene other are several: long-grained nonglutinous rice, java rice or wild rice material, as donor parents, also can produce identical effect, in this explanation no longer one by one.
In addition, all breeding materials that relate in the present invention all can be commercially available.
Finally it should be noted that: the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, its technical scheme that still can record previous embodiment is modified, or part technical characterictic is wherein equal to replacement.Within the spirit and principles in the present invention all, any amendment of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (9)
1. a long grain Japonica Hybrid restorer selection, is characterized in that, comprises the following steps:
(1) select to have long grain type paddy rice resource material fertility, grain length >=6.5mm, as the donor parents of grain type character gene;
(2) donor parents and the hybridization of traditional round shaped grain type Restoring Line of Japonica Rice of long grain type gene will be carried;
(3) separate in offspring the individual plant results seed of initial option rice grain length >=6.5mm in hybridization;
(4) with the long grain japonica rice material and the japonica rice sterile test cross that obtain in step (3), measure that it is restorative, select the strain that wherein recovery capability is good, economical character is good.
2. long grain Japonica Hybrid restorer selection according to claim 1, is characterized in that, the long grain type paddy rice resource material in described step (1) is japonica rice, long-grained nonglutinous rice, java rice or the wild rice material of long grain.
3. long grain Japonica Hybrid restorer selection according to claim 1, is characterized in that, the traditional round shaped grain type Restoring Line of Japonica Rice in described step (2) is traditional round shaped grain type Restoring Line of Japonica Rice of rice grain length 5.0-5.5mm; Or there is part recovery capability, and on output, quality, resistance, economical character, there is advantage, traditional round shaped grain japonica rice material of rice grain length 5.0-5.5mm.
4. long grain Japonica Hybrid restorer selection according to claim 1, it is characterized in that, in described step (3), the long grain japonica rice material obtaining through initial option backcrosses 1-2 time with the donor parents of carrying long grain type gene again, separate in offspring at it, further select the individual plant results seed of rice grain length >=6.5mm.
5. long grain Japonica Hybrid restorer selection according to claim 1, it is characterized in that, in described step (3), the long grain japonica rice material obtaining through initial option is reestablished diplomatic relations or reestablishes diplomatic relations and backcross with the material with other excellent proterties again, make other beneficial genes and long kernel Characters polymerization, then separate from it individual plant results seed of further selecting rice grain length >=6.5mm offspring.
6. a long grain Japonica Hybrid restorer selection according to claim 5, is characterized in that, described in there are other excellent proterties material be japonica rice, long-grained nonglutinous rice, java rice or wild rice material.
7. according to the long grain Japonica Hybrid restorer selection described in claim 1-6 any one, it is characterized in that, described separation offspring is F2-F5 generation.
8. long grain Japonica Hybrid restorer selection according to claim 1, is characterized in that, the japonica rice sterile in described step (4) is Yunnan I type, BT type, seal water type and/or wild abortion type.
9. long grain Japonica Hybrid restorer selection according to claim 1, is characterized in that, repeating step (4) is further verified its restorative and economical character, obtains the good long grain Restoring Line of Japonica Rice of economical character.
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Families Citing this family (9)
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| CN105993930A (en) * | 2016-05-12 | 2016-10-12 | 安徽理想种业有限公司 | Breeding method for long grain type high temperature resistance conventional japonica rice variety |
| CN106489721B (en) * | 2016-10-28 | 2018-09-28 | 福建金山都发展有限公司 | A kind of two-line sterile line selection of the high-quality short round shaped grain type of rice |
| CN107960319A (en) * | 2017-11-23 | 2018-04-27 | 安徽省绿福农业科技股份有限公司 | A kind of long grain round-grained rice rice variety selective and screening technique |
| CN108770678B (en) * | 2018-04-16 | 2021-07-16 | 陈日胜 | Cross breeding and breeding method of sea rice and japonica rice restorer |
| CN109349102A (en) * | 2018-12-11 | 2019-02-19 | 中国水稻研究所 | A kind of long grain japonica rice two-line sterile line selection |
| CN109287475A (en) * | 2018-12-11 | 2019-02-01 | 中国水稻研究所 | A kind of long grain Japonica Hybrid selection of two systems |
| CN109287478A (en) * | 2018-12-11 | 2019-02-01 | 中国水稻研究所 | A kind of long particle shape two-line sterile line of rice selection |
| CN112522279B (en) * | 2020-11-19 | 2021-12-14 | 厦门大学 | Coding sequence of rice grain type gene OsGL8 gene and application |
| CN113261500A (en) * | 2021-05-07 | 2021-08-17 | 湖南杂交水稻研究中心 | Breeding method of special ultrahigh-yield hybrid rice for brewing Luzhou-flavor liquor |
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| CN101019505A (en) * | 2007-03-22 | 2007-08-22 | 江苏丘陵地区镇江农业科学研究所 | Breeding process of rice blast-resisting high quality and high yield round-grained rice variety Zhendao-10 |
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| US6953881B2 (en) * | 2003-09-30 | 2005-10-11 | Ricetec, Ag | Rice hybrid RH103 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101019505A (en) * | 2007-03-22 | 2007-08-22 | 江苏丘陵地区镇江农业科学研究所 | Breeding process of rice blast-resisting high quality and high yield round-grained rice variety Zhendao-10 |
Non-Patent Citations (3)
| Title |
|---|
| 优质长粒粳稻恢复系C470及其改良利用;张晓东等;《中国农业科技导报》;20091231;第11卷(第S2期);第85-87页 * |
| 张晓东等.优质长粒粳稻恢复系C470及其改良利用.《中国农业科技导报》.2009,第11卷(第S2期),第85-87页. |
| 黄海祥，章善庆.长粒粳稻不育系嘉禾212A育成.《杂交水稻》.2005,第20卷(第1期),第19页. * |
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