CN109287475A - A kind of long grain Japonica Hybrid selection of two systems - Google Patents
A kind of long grain Japonica Hybrid selection of two systems Download PDFInfo
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- CN109287475A CN109287475A CN201811510300.9A CN201811510300A CN109287475A CN 109287475 A CN109287475 A CN 109287475A CN 201811510300 A CN201811510300 A CN 201811510300A CN 109287475 A CN109287475 A CN 109287475A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
A kind of long grain Japonica Hybrid selection of two systems, belongs to hybrid rice Biotechnology in Genetic Breeding field.It is the following steps are included: the long grain japonica rice two-line sterile line of breeding；Long grain Restoring Line of Japonica Rice is selected to hybridize for parent with domestic backbone long-grained nonglutinous rice or Restoring Line of Japonica Rice and be returned using round-grained rice parent as female parent, separating the two of the long grain shape of progeny selection at it is Restoring Line of Japonica Rice or Xian round-grained rice osculant restorer；It is female parent with long grain japonica rice two-line sterile line, long grain shape two is that the long grain restorer of Restoring Line of Japonica Rice or long grain Restoring Line of Japonica Rice or introduction is that male parent is measured, and breeding obtains the long grain Japonica Hybrid new varieties of two line method.The present invention is suitable for carrying out grain shape improvement to existing two line japonica hybrid rice, the round shaped grain type two line japonica hybrid rice for changing a length of 5.0-5.5mm of traditional rice grain is two system head's grain shape Japonica Hybrid of rice grain length >=6.5mm, rice appearance, quality and the hybrid seed yield etc. of two line japonica hybrid rice are significantly improved, Developing Japonica Hybrid Rice is promoted.
Description
Technical field
The invention belongs to hybrid rice Biotechnology in Genetic Breeding fields, and in particular to a kind of long grain Japonica Hybrid breeding side of two systems
Method.
Background technique
China's hybrid rice technology and application are advanced in the world, and it is more than 200,000,000 mu that year, which plants hybrid rice, predominantly hybridization Xian
Rice, including three line method and two line method.1981, China was put forward for the first time the production of hybrid seeds under long day high temperature, numerous under short day low temperature in the world
Kind, one is the imagination (Shi Mingsong, 1981) of dual-purpose breeding hybrid paddy rice, the pioneering double-hybrid rice strains technical research in China and benefit
With in acquisition national science and technology progress special award in 2013, achievement was also mainly reflected on two line method indica Hybrid Rice.It is miscellaneous two
In terms of handing over japonica rice, for decades, rice in China breeder has cultivated two line japonica hybrid rice sterile line 39, two line japonica hybrid rice
40 (national rice data center, http://www.ricedata.cn/), also develop for the developing of China's two line japonica hybrid rice
Certain basis is established.
Compared to indica Hybrid Rice, the development of Japonica Hybrid is very slow, and Japonica Hybrid year plantation in the past 10 years is only at 3,000,000 mu
Left and right is hovered, especially only 700,000 mu or so of two line japonica hybrid rice cultivated area.It is similar with traditional japonica rice (also to be limited by traditional understanding
System, it is believed that the long short grain of grain japonica rice of long-grained nonglutinous rice；Cheng Kansheng, 1984), the japonica rice two-line sterile line that China is bred as so far, such as
7001S, N5088S etc. are short grain granule (rice grain length 5.0-5.5mm, length-width ratio is 2.0 or so), correspondingly, in production
The two line japonica hybrid rice of application is also short grain granule (rice grain length 5.0-5.5mm), and sterile line flowering habit is poor, the production of hybrid seeds
Relative difficulty, the long grain appearance welcome with market does not match that, limits the development of Japonica Hybrid to a certain extent.
To innovate japonica rice grain shape, promote Developing Japonica Hybrid Rice, before our long grain Japonica Hybrid (ZL of invention three line method
201210152095.X), the long grain shape Japonica Hybrid of the multiple three line methods of Zhejiang Province's breeding in recent years (or inclined round-grained rice), as Jiahe is excellent
001, Feng You 2 is praised, excellent No. 2 long etc., the good-looking mouthfeel of the long grain of appearance is excellent, is received by the market.
In this context, we continue to invent a kind of long grain Japonica Hybrid selection of two systems, and initiative develops long grain shape two
It is Japonica Hybrid, significantly improves the appearance and market quality of existing Japonica Hybrid, adapt to market and citizen requirement, promotes hybridization round-grained rice
Rice development.
Summary of the invention
In view of the problems of the existing technology, present invention design provides a kind of skill of the long grain Japonica Hybrid selection of two systems
Art scheme.What the present invention obtained promotes two system head's grain shape Japonica Hybrid kinds for cultivating, and can improve existing double-line hybrid round-grained rice
The appearance and market quality of rice adapt to domestic and international market demand and urban and rural residents' eating requirements, it can also be used to it is long to develop two line method
Grain shape Japonica Hybrid seed industry.
The long grain Japonica Hybrid selection of two system of one kind, it is characterised in that the following steps are included:
1) the long grain japonica rice two-line sterile line of breeding；
2) the long grain Restoring Line of Japonica Rice for selecting patent No. ZL201210152095.X to be related to is parent and domestic backbone long-grained nonglutinous rice or round-grained rice
Rice restorer hybridize and be returned using round-grained rice parent as female parent, and separating the two of the long grain shape of progeny selection at it is that japonica rice is extensive
Multiple system or Xian round-grained rice osculant restorer；
3) with the long grain japonica rice two-line sterile line of step 1) be female parent, the long grain shape two of step 2 be Restoring Line of Japonica Rice or
The long grain restorer of long grain Restoring Line of Japonica Rice or introduction that ZL201210152095.X is related to is that male parent is measured, and breeding obtains
The long grain Japonica Hybrid new varieties of two line method.
The long grain Japonica Hybrid selection of two system of one kind, it is characterised in that long grain japonica rice two in the Dun step 1)
It is sterile line breeding step are as follows:
A) the long grain japonica rice that selection patent No. ZL 201210152095.X and patent No. ZL 201210152908.5 is related to restores
System and holding based material, as receptor or donor parents；
B) select the indica type two-line sterile line with excellent economical character as donor or receptor parent, the indica type two systems infertility
The sterile gene of system comes from Nongken 58S and An Nong S；
C) step a) is hybridized with the rice material of step b), is then returned using step 1) as male parent or female parent
It hands over, reestablish diplomatic relations 1-2 times, making long grain japonica rice gene and two is that sterile gene or other beneficial genes recombinate；
D) offspring is separated in several backcrossings that step c) obtains, and selects two system's infertility single plant of rice grain length >=6.5mm or more
Harvest seed；
E) the long grain japonica rice infertility single plant that plantation step d) is obtained, it is continuous to be selfed or be returned 1-3 times, obtain stable long grain japonica rice
Two-line sterile line material；
F) light temperature culture identification, Xian round-grained rice assessment of indices, round-grained rice are carried out to the long grain japonica rice two-line sterile line material that step e) obtains
Index > 0.75, economical character selection, to obtain long grain japonica rice two-line sterile line.
The long grain Japonica Hybrid selection of two system of one kind, it is characterised in that long grain japonica rice is extensive in the step 2)
Multiple system includes L917, L42 or their breeding Derivative line and intermediate materials；Domestic backbone long-grained nonglutinous rice or Restoring Line of Japonica Rice include the country
It is bred as the restorer of application；Two be rice grain length >=6.5mm of Restoring Line of Japonica Rice or Xian round-grained rice osculant restorer.
The long grain Japonica Hybrid selection of two system of one kind, it is characterised in that maternal and male parent in the step 3)
It is all long grain, rice grain length >=6.5mm.
The long grain Japonica Hybrid selection of two system of one kind, it is characterised in that the long grain of two line method in the step 3)
Rice grain length >=6.5mm of Japonica Hybrid new varieties.
It is of the invention to be advantageous in that: to be suitable for carrying out grain shape improvement to existing two line japonica hybrid rice, change traditional rice grain
The round shaped grain type two line japonica hybrid rice of a length of 5.0-5.5mm is two system head's grain shape Japonica Hybrid of rice grain length >=6.5mm.With long grain
Type japonica rice sterile (rice grain length >=6.5mm) and long grain shape Restoring Line of Japonica Rice or the long grain restorer (rice grain of Xian round-grained rice osculant
Length >=6.5mm) combo, the long grain Japonica Hybrid Combination nova (kind) of two systems of rice grain length >=6.5mm is cultivated, two systems are significantly improved
Rice appearance, quality and hybrid seed yield of Japonica Hybrid etc. promote Developing Japonica Hybrid Rice.
Specific embodiment
Further illustrate the present invention with reference to embodiments.
A kind of embodiment 1: long grain japonica rice two-line sterile line selection
1) the long grain japonica rice that selection patent No. ZL 201210152095.X and patent No. ZL 201210152908.5 is related to restores
System and holding based material, preferably long grain Restoring Line of Japonica Rice are L917, L42 or their breeding Derivative line and intermediate materials, are protected
Holding based material is long round-grained rice 1B, long round-grained rice 2B or their breeding Derivative line and intermediate materials, as receptor or donor parents；
2) select the indica type two-line sterile line with excellent economical character as donor or receptor parent, the indica type two systems infertility
The sterile gene of system comes from Nongken 58S and An Nong S, preferably Peiai 64S and Y58S；
3) step 1) is hybridized with the rice material of step 2, is then returned using step 1) as male parent or female parent
It hands over, reestablish diplomatic relations 1-2 times, making long grain japonica rice gene and two is that sterile gene or other beneficial genes recombinate；
4) offspring is separated in several backcrossings that step 3) obtains, and selects two system's infertility single plant of rice grain length >=6.5mm or more
Harvest seed；
5) plantation step 4) the long grain japonica rice infertility single plant that obtains, continuous selfing or backcrossing 1-3 times obtain stable long grain japonica rice
Two-line sterile line material；
Step 4) and 5) middle acquisition rice grain length >=6.5mm selection are two kinds: one in once hybridization segregating population
Middle selection has the rice single plant of long kernel Characters, it is desirable that selects in rice grain length >=6.5mm or more single plant, then reselection agronomy
The excellent single plant of character, obtaining long grain japonica rice two is sterile material；Two be that the long grain japonica rice material for obtaining primary election further returns
It hands over, polymerize other beneficial genes with long kernel Characters, obtaining long grain japonica rice two is sterile material；
6) light temperature culture identification (manually climate box identification length is carried out to the long grain japonica rice two-line sterile line material that step 5) obtains
Grain japonica rice two is the Fertility Transfer Temperature of sterile material), Xian round-grained rice assessment of indices (is measured with the molecular labeling of difference Xian round-grained rice difference
Be bred as the Indica or japonica of material, the selection of economical character meets Production of Large Fields requirement), round-grained rice sex index > 0.75, economical character choosing
It selects, to obtain long grain japonica rice two-line sterile line.
Application examples 1:
The cultivation of the long long round-grained rice 1S of grain Japonica Hybrid:
(1) it selects indica type two-line sterile line Peiai 64S to keep being that long round-grained rice 1B hybridizes with long grain japonica rice, is returned in the long round-grained rice 1B of its F1 generation
It hands over, the isolated F2-F4 of plantation hybridization from generation to generation, selects long two system's infertility single plant of kernel Characters, continuous to plant the stable length of 2-3 generation acquisition
Grain japonica rice two is sterile material.The identification of light temperature, the identification of Xian round-grained rice index, economical character selection are carried out by growth cabinet to it,
Cultivate the long round-grained rice 1S of long grain japonica rice two-line sterile line.
(2) long round-grained rice 1S stable fertility shows high temperature-induced sterility, and low temperature is fertile, and Fertility Transfer Temperature is 23.5 DEG C；Round-grained rice 1S is opened to open
Flower habit is good, stigma exposing ratio is high, outcrossing seed-setting rate is high；Through Xian round-grained rice assessment of indices, long round-grained rice 1S round-grained rice sex index 0.91, for typical round-grained rice
Rice；Long round-grained rice 1S long grain shape (rice grain length 7.2mm, length-width ratio 3.2), appearance and rice matter index are excellent, and breeding time is suitable, economical character
Excellent, field planting shows blast resisting.
(3) long round-grained rice 1S and self-fertile restorer combo, long grain Japonica Hybrid Combination nova (kind) cell of two systems of preparation are utilized
Experimental cultivation shows stable high yield, and rice grain length 7.1mm, blast resisting, economical character is excellent, prepares application and participates in provincial region
Test.
Application examples 2:
The cultivation of the long long round-grained rice 3S of grain Japonica Hybrid
(1) indica type two-line sterile line Y58S is selected to hybridize with the Derivative line D650 of long grain Restoring Line of Japonica Rice material L917, in its F1
Alternative D650 backcrossing, plantation hybridize isolated F2-F4 from generation to generation, select long two system's infertility single plant of kernel Characters, continuously plant 2-3 generation and obtain
Obtaining stable long grain japonica rice two is sterile material.The identification of light temperature, the identification of Xian round-grained rice index, agronomy are carried out by growth cabinet to it
Character determination cultivates the long round-grained rice 3S of long grain japonica rice two-line sterile line.
(2) long round-grained rice 3S stable fertility shows high temperature-induced sterility, and low temperature is fertile, and Fertility Transfer Temperature is 23.2 DEG C；Round-grained rice 1S is opened to open
Flower habit is good, stigma exposing ratio is high, outcrossing seed-setting rate is high；Through Xian round-grained rice assessment of indices, long round-grained rice 1S round-grained rice sex index 0.87, for typical round-grained rice
Rice；Long round-grained rice 3S long grain shape (rice grain length 7.5mm, length-width ratio 3.3), appearance and rice matter index are excellent, and breeding time is suitable, economical character
It is excellent, there is fragrance.
(3) long round-grained rice 3S and self-fertile restorer combo, long grain Japonica Hybrid Combination nova (kind) plot experiment of two systems of preparation
Planted stable high yield, economical character is excellent, and setting percentage is high, and exterior quality is all good with mouthfeel, shows preferable application prospect.
The long round-grained rice 1S grain shape of the long japonica rice two-line sterile line of table 1 and Main Agronomic characteristic
The long round-grained rice 3S grain shape of the long japonica rice two-line sterile line of table 2 and Main Agronomic characteristic
。
A kind of embodiment 2: the long grain Japonica Hybrid selection of two systems
1) the long grain japonica rice two-line sterile line of method breeding recorded by embodiment 1；
2) the long grain Restoring Line of Japonica Rice for selecting patent No. ZL201210152095.X to be related to is parent and domestic backbone long-grained nonglutinous rice or round-grained rice
Rice restorer hybridize and be returned using round-grained rice parent as female parent, and separating the two of the long grain shape of progeny selection at it is that japonica rice is extensive
Multiple system or Xian round-grained rice osculant restorer, as preferred long grain Restoring Line of Japonica Rice include L917, L42 or their breeding Derivative line and
Intermediate materials；Domestic backbone long-grained nonglutinous rice or Restoring Line of Japonica Rice include the domestic restorer for being bred as application；Two be Restoring Line of Japonica Rice or Xian
Rice grain length >=6.5mm of round-grained rice osculant restorer；
3) with the long grain japonica rice two-line sterile line of step 1) be female parent, the long grain shape two of step 2 be Restoring Line of Japonica Rice or
The long grain restorer of long grain Restoring Line of Japonica Rice or introduction that ZL201210152095.X is related to is that male parent is measured, maternal and father
This is all long grain, and rice grain length >=6.5mm, breeding obtains the long grain Japonica Hybrid new varieties of two line method；
4) the long grain Japonica Hybrid new varieties of two systems formed to step 3) carry out economical character measurement, yield, quality, resistance, system
The identifications such as kind yield participate in area's examination or association's test, and then pass through authorization and promoted.
Application examples 3
The cultivation of the long round-grained rice of the long grain Japonica Hybrid of two systems two excellent 1:
(1) the long round-grained rice 1S of long grain japonica rice two-line sterile line for taking application examples 1 to obtain；
(2) the long grain Restoring Line of Japonica Rice L917 for selecting ZL201210152095.X to be related to is parent and domestic backbone Indica Rice Restorer Lines
China, which accounts for, to be hybridized, and carries out backcrossing 2 times by female parent of round-grained rice parent L917, in its separation long grain shape of progeny selection (rice grain length
>=6.5mm) two be Restoring Line of Japonica Rice L113；
(3) it using the restorer L113 combo of long grain japonica rice two-line sterile line long round-grained rice 1S and breeding, prepares the long grain of two systems and hybridizes round-grained rice
The long round-grained rice two excellent 1 (long round-grained rice 1S/L113) of rice Combination nova, the two excellent No. 1 plot experiment Planted stable high yield of long round-grained rice, rice grain length
7.1mm, blast resisting, economical character is excellent, prepares application and participates in provincial region test.
Application examples 4
The cultivation of the long round-grained rice of the long grain Japonica Hybrid of two systems two excellent 3
(1) the long round-grained rice 3S of long grain japonica rice two-line sterile line for taking application examples 2 to obtain.
(2) the long grain Restoring Line of Japonica Rice L917 for selecting ZL201210152095.X to be related to is extensive for parent and domestic backbone long-grained nonglutinous rice
Again it is that China accounts for and hybridized, is that female parent carries out backcrossing 2 times using round-grained rice parent L917, in its separation long grain shape (rice of progeny selection
Grain length >=6.5mm) two be Restoring Line of Japonica Rice L113.
(3) using the restorer L113 combo of long grain japonica rice two-line sterile line long round-grained rice 3S and breeding, it is miscellaneous to prepare the long grain of two systems
Hand over the long round-grained rice two excellent 3 (long round-grained rice 3S/L113) of japonica rice Combination nova, the two excellent No. 3 plot experiment Planted stable high yield of long round-grained rice, rice
Grain length 7.3mm, blast resisting, economical character is excellent, and rice has fragrance, prepares application and participates in provincial region test.
3 liang of table are long grain Japonica Hybrid grain shape and yield traits
。
Claims (5)
1. a kind of long grain Japonica Hybrid selection of two systems, it is characterised in that the following steps are included:
1) the long grain japonica rice two-line sterile line of breeding；
2) the long grain Restoring Line of Japonica Rice for selecting patent No. ZL201210152095.X to be related to is parent and domestic backbone long-grained nonglutinous rice or round-grained rice
Rice restorer hybridize and be returned using round-grained rice parent as female parent, and separating the two of the long grain shape of progeny selection at it is that japonica rice is extensive
Multiple system or Xian round-grained rice osculant restorer；
3) with the long grain japonica rice two-line sterile line of step 1) be female parent, the long grain shape two of step 2 be Restoring Line of Japonica Rice or
The long grain restorer of long grain Restoring Line of Japonica Rice or introduction that ZL201210152095.X is related to is that male parent is measured, and breeding obtains
The long grain Japonica Hybrid new varieties of two line method.
2. the long grain Japonica Hybrid selection of two systems of one kind as described in claim 1, it is characterised in that in the Dun step 1)
Long grain japonica rice two-line sterile line breeding step are as follows:
A) the long grain japonica rice that selection patent No. ZL 201210152095.X and patent No. ZL 201210152908.5 is related to restores
System and holding based material, as receptor or donor parents；
B) select the indica type two-line sterile line with excellent economical character as donor or receptor parent, the indica type two systems infertility
The sterile gene of system comes from Nongken 58S and An Nong S；
C) step a) is hybridized with the rice material of step b), is then returned using step 1) as male parent or female parent
It hands over, reestablish diplomatic relations 1-2 times, making long grain japonica rice gene and two is that sterile gene or other beneficial genes recombinate；
D) offspring is separated in several backcrossings that step c) obtains, and selects two system's infertility single plant of rice grain length >=6.5mm or more
Harvest seed；
E) the long grain japonica rice infertility single plant that plantation step d) is obtained, it is continuous to be selfed or be returned 1-3 times, obtain stable long grain japonica rice
Two-line sterile line material；
F) light temperature culture identification, Xian round-grained rice assessment of indices, round-grained rice are carried out to the long grain japonica rice two-line sterile line material that step e) obtains
Index > 0.75, economical character selection, to obtain long grain japonica rice two-line sterile line.
3. the long grain Japonica Hybrid selection of two systems of one kind as described in claim 1, it is characterised in that in the step 2)
Long grain Restoring Line of Japonica Rice includes L917, L42 or their breeding Derivative line and intermediate materials；Domestic backbone long-grained nonglutinous rice or japonica rice restore
System includes the domestic restorer for being bred as application；Two be rice grain length >=6.5mm of Restoring Line of Japonica Rice or Xian round-grained rice osculant restorer.
4. the long grain Japonica Hybrid selection of two systems of one kind as described in claim 1, it is characterised in that in the step 3)
Maternal and male parent is all long grain, rice grain length >=6.5mm.
5. the long grain Japonica Hybrid selection of two systems of one kind as described in claim 1, it is characterised in that in the step 3)
Rice grain length >=6.5mm of the long grain Japonica Hybrid new varieties of two line method.
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| CN113243293A (en) * | 2021-06-23 | 2021-08-13 | 武汉亘谷源生态农业科技有限公司 | Breeding method of super hybrid rice between subspecies of indica rice and japonica rice |
| CN113661916A (en) * | 2021-06-29 | 2021-11-19 | 高邮周邶墩农业发展有限公司 | Breeding method of long-grain indica-japonica intermediate wide-affinity restorer line |
| CN113661916B (en) * | 2021-06-29 | 2022-05-06 | 高邮周邶墩农业发展有限公司 | Breeding method of long-grain indica-japonica intermediate wide-affinity restorer line |
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