CN102657077A - Long-grain hybrid japonica rice restorer selection method - Google Patents
Long-grain hybrid japonica rice restorer selection method Download PDFInfo
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Abstract
The invention discloses a long-grain hybrid japonica rice restorer selection method comprising the following steps of: firstly, selecting long-grain rice resource materials as donor parents of grain-type trait genes; secondly, hybridizing the donor parents carrying the long-grain genes with the conventional round-grain japonica rice restorers; thirdly, initially selecting single plants with the rice grain length being more than 6.5mm to harvest seeds in separation of hybrid progenies; and fourthly, performing test-cross on the long-grain japonica rice materials obtained from the step 3 and the sterile lines of japonica rice to test the restoration of the long-grain japonica rice materials, and selecting strains with good restorability and excellent agronomic traits. The selection method is applicable to perform a grain type improvement on the conventional round-grain japonica rice restorer to create the long-grain hybrid japonica rice, can change the conventional round grains of 5.0mm to 5.5mm to the long grains more than or equal to 6.5mm, and remarkably improves the appearance and quality of the rice.
Description
Technical field
The present invention relates to Japonica Hybrid genetic breeding field, particularly, relate to long grain type Japonica Hybrid and recover system selective breeding method.
Background technology
The Asia cultivated rice has two subspecies of long-grained nonglutinous rice japonica rice, and Typical Japonica is most to be short grain round shaped grain (rice grain length 5.0-5.5mm), a small amount of long grain type japonica rice resource is arranged, but do not have recovery capability.Up to now; The Japonica Hybrid breeding is produced and gone up the japonica rice recovery of using is that it recovers gene source in long-grained nonglutinous rice; Be short grain round shaped grain (rice grain length 5.0-5.5mm) type; Make present Japonica Hybrid not possess long grain appearance and the quality that welcome in market, affect the production development of Japonica Hybrid to a certain extent.Under this background, invent a kind of selection that a japonica rice recovers system of growing, can significantly improve existing Japonica Hybrid and recover the outward appearance and the market quality of system, the long grain of initiative type Japonica Hybrid.
Summary of the invention
The object of the present invention is to provide a kind of selection that Japonica Hybrid is recovered system of growing, it can be improved existing japonica rice and recover outward appearance and the market quality of system, long type Japonica Hybrid kind of seed selection, and use it for the production of hybrid seeds production of Japonica Hybrid.
To achieve these goals, the present invention adopts following technical scheme:
A kind of long grain Japonica Hybrid is recovered system selective breeding method, may further comprise the steps:
(1) selects long grain type paddy rice resource material, as the donor parents of grain type character gene;
(2) donor parents and the traditional round shaped grain type japonica rice that will carry long grain type gene recover system's hybridization;
(3) separate among the offspring individual plant results seed that initial option rice grain length >=6.5mm is above in hybridization;
(4) with the long grain japonica rice material and japonica rice sterile test cross that obtain in the step (3), measure that it is restorative, select the strain that wherein recovery capability is good, economical character is good to be.
Further, the long grain type paddy rice resource material in the said step (1) is japonica rice, long-grained nonglutinous rice, java rice or the wild rice material of long grain.
Further, the grain length >=6.5mm of the long grain type paddy rice resource material in the said step (1).
Further, the traditional round shaped grain type japonica rice in the said step (2) recovers system and recovers system for traditional round shaped grain type japonica rice of rice grain length 5.0-5.5mm; Or have the part recovery capability, and on output, quality, resistance, economical character, has advantage, traditional round shaped grain japonica rice material of rice grain length 5.0-5.5mm.
Further, in the said step (3), can backcross 1-2 time with the donor parents of carrying long grain type gene again, separate among the offspring, further select the above individual plant results seed of rice grain length >=6.5mm at it through the long grain japonica rice material that initial option obtains.
Further; In the said step (3); Long grain japonica rice material through initial option obtains can be reestablished diplomatic relations or reestablish diplomatic relations and backcross with the material with other excellent proterties again; Make other beneficial genes and the polymerization of long graininess shape, separate the individual plant results seed of further selecting the offspring more than rice grain length >=6.5mm from it again.
Further, said material with other excellent proterties is japonica rice, long-grained nonglutinous rice, java rice or wild rice material.
Further, said separation offspring is F2-F5 generation.
Further, the japonica rice sterile in the said step (4) is Yunnan I type, BT type, seal water type and/or wild abortion type.
Further, repeating step (4) is further verified its restorative and economical character, obtains the good long grain japonica rice of economical character and recovers system.
The present invention has following beneficial effect owing to adopt technique scheme:
The present invention through select suitable breeding material as the parent, adopt hybridize, reestablish diplomatic relations, backcross or the long grain of multiple breeding method seed selection Japonica Hybrid such as its combination to recover be to verify its recovery capability exactly through test cross again.Selection of the present invention is applicable to that existing japonica rice is recovered system carries out a type improvement, the long grain of initiative type Japonica Hybrid, and the round shaped grain type that changes traditional 5.0-5.5mm significantly improves rice outward appearance and quality for the long grain type of >=6.5mm.。
Embodiment
A kind of long grain Japonica Hybrid of the present invention is recovered system selective breeding method, may further comprise the steps:
(1) selects long grain type paddy rice resource material, as the donor parents of grain type character gene;
(2) donor parents that will carry long grain type gene is recovered system with traditional round shaped grain japonica rice and hybridize, backcrosses, reestablishes diplomatic relations, and long grain type gene, recovery gene and other beneficial genes are recombinated;
(3), select the above individual plant results seed of rice grain length >=6.5mm at the separation Offspring F2-F5 of several kinds of hybridization modes;
(4) with the long grain japonica rice material and japonica rice sterile test cross that obtain in the step (3), measure that it is restorative, select the strain that wherein recovery capability is good, economical character is good to be;
(5) repeating step (4) is verified its restorative and economical character, obtains the good long grain japonica rice of economical character and recovers system.
Long grain type paddy rice resource material in the above-mentioned steps (1) is japonica rice, long-grained nonglutinous rice, java rice or the wild rice material of long grain;
Traditional round shaped grain japonica rice in the above-mentioned steps (2) recovers system and recovers system for traditional round shaped grain type japonica rice of rice grain length 5.0-5.5mm; Or have the part recovery capability, and on output, quality, resistance, economical character, has traditional round shaped grain japonica rice material of advantage, rice grain length 5.0-5.5mm.
The recovery system, method that obtains seed grain length >=6.5mm in the above-mentioned steps (4) has and has two kinds at least: one for once hybridizing the individual plant of selecting to have long graininess shape in the segregation population; Require to select in the above individual plant of rice grain length >=6.5mm; And then select plant type and the good individual plant of economical character, the long grain japonica rice that obtains artificial modified recovers system; Two is that long the japonica rice material that primary election obtains reestablished diplomatic relations and/or backcrossed with the material with other excellent proterties, makes other beneficial genes and the polymerization of long graininess shape, obtains the length grain japonica rice recovery system of artificial modified.Wherein, the material that has other excellent proterties is japonica rice, long-grained nonglutinous rice, java rice or wild rice material.
Japonica rice sterile in the above-mentioned steps (4) comprises Yunnan I type, BT type, seal water type and/or wild abortion type;
The long grain japonica rice that obtains in above-mentioned steps (4), (5) recovers system, and rice grain length >=6.5mm can be used for preparing hybrid japonica rice new varieties, also can directly be used for the production of hybrid seeds production of Japonica Hybrid combination.
Be further elaborated by way of example below:
Embodiment 1
It is the seed selection of L81 that long grain japonica rice recovers: selecting japonica rice to recover is C418 and long grain japonica rice material Jiahe 212 hybridization; Growing graininess shape individual plant from generation to generation at filial generation F2-F5 selects; With BT type male sterile line Zhejiang round-grained rice 2A, Yunnan I type male sterile line spring river 12A test cross, choosing in test cross F1 that high, restorative good, the long grain type (rice grain length 6.6mm) of hybrid ripening rate, economical character long grain japonica rice good, blast resisting recover is L81.
Embodiment 2
It is the seed selection of L42 that long grain japonica rice recovers: selecting japonica rice to recover is C418 and long grain japonica rice material Jiahe 212 hybridization; Hybridization F2 segregative generation select the elongated economical character of grain type preferably individual plant again with long grain type germ plasm resource CN109 hybridization; Growing graininess shape individual plant from generation to generation at the F2-F5 that reestablishes diplomatic relations selects; With BT type male sterile line Zhejiang round-grained rice 2A, Yunnan I type male sterile line spring river 12A test cross, choosing in test cross F1 that high, restorative good, the long grain type (rice grain length 7.2mm) of hybrid ripening rate, economical character long grain japonica rice good, blast resisting recover is L42.
Embodiment 3
It is the seed selection of L917 that long grain japonica rice recovers: selecting japonica rice to recover is C418 and long grain japonica rice material Jiahe 212 hybridization; Hybridization F2 segregative generation select the elongated economical character of grain type preferably individual plant to recover with long-grained nonglutinous rice be R838 hybridization again; In the F2 segregation population of reestablishing diplomatic relations, further select the elongated economical character of grain type preferably individual plant backcross with parent Jiahe 212 again; Growing graininess shape individual plant from generation to generation at the F2-F5 that reestablishes diplomatic relations selects; With BT type male sterile line Zhejiang round-grained rice 2A, Yunnan I type male sterile line spring river 12A test cross, choosing in test cross F1 that high, restorative good, the long grain type (rice grain length 6.9mm) of hybrid ripening rate, the good long grain japonica rice of economical character recover is L917.
The long japonica rice of table 1 recovers system and tradition is recovered system's grain type and yield traits compares
The foregoing description 1-3 adopts long grain type japonica rice variety as donor parents; In like manner, can also select to have other several kinds that grow grain type gene: long-grained nonglutinous rice, java rice or wild rice material, as donor parents; Also can produce identical effect, in this explanation no longer one by one.
In addition, all breeding materials that relate among the present invention all can be commercially available.
What should explain at last is: the above is merely the preferred embodiments of the present invention; Be not limited to the present invention; Although the present invention has been carried out detailed explanation with reference to previous embodiment; For a person skilled in the art, it still can be made amendment to the technical scheme that previous embodiment is put down in writing, and perhaps part technical characterictic wherein is equal to replacement.All within spirit of the present invention and principle, any modification of being done, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. one kind long grain Japonica Hybrid is recovered system selective breeding method, it is characterized in that, may further comprise the steps:
(1) selects long grain type paddy rice resource material, as the donor parents of grain type character gene;
(2) donor parents and the traditional round shaped grain type japonica rice that will carry long grain type gene recover system's hybridization;
(3) separate among the offspring individual plant results seed that initial option rice grain length >=6.5mm is above in hybridization;
(4) with the long grain japonica rice material and japonica rice sterile test cross that obtain in the step (3), measure that it is restorative, select the strain that wherein recovery capability is good, economical character is good to be.
2. long grain Japonica Hybrid according to claim 1 is recovered system selective breeding method, it is characterized in that, the long grain type paddy rice resource material in the said step (1) is japonica rice, long-grained nonglutinous rice, java rice or the wild rice material of long grain.
3. long grain Japonica Hybrid according to claim 1 is recovered system selective breeding method, it is characterized in that the grain length >=6.5mm of the long grain type paddy rice resource material in the said step (1).
4. long grain Japonica Hybrid according to claim 1 is recovered system selective breeding method, it is characterized in that, the traditional round shaped grain type japonica rice in the said step (2) recovers system and recovers system for traditional round shaped grain type japonica rice of rice grain length 5.0-5.5mm; Or have the part recovery capability, and on output, quality, resistance, economical character, has advantage, traditional round shaped grain japonica rice material of rice grain length 5.0-5.5mm.
5. long grain Japonica Hybrid according to claim 1 is recovered system selective breeding method; It is characterized in that; In the said step (3); Long grain japonica rice material through initial option obtains can be backcrossed 1-2 time with the donor parents of carrying long grain type gene again, separates among the offspring at it, further selects the above individual plant results seed of rice grain length >=6.5mm.
6. long grain Japonica Hybrid according to claim 1 is recovered system selective breeding method; It is characterized in that; In the said step (3); Long grain japonica rice material through initial option obtains can be reestablished diplomatic relations or reestablish diplomatic relations and backcross with the material with other excellent proterties again, makes other beneficial genes and the polymerization of long graininess shape, separates the individual plant results seed of further selecting the offspring more than rice grain length >=6.5mm from it again.
7. long grain Japonica Hybrid according to claim 6 is recovered system selective breeding method, it is characterized in that said material with other excellent proterties is japonica rice, long-grained nonglutinous rice, java rice or wild rice material.
8. recover system selective breeding method according to each described long grain Japonica Hybrid of claim 1-7, it is characterized in that said separation offspring is F2-F5 generation.
9. long grain Japonica Hybrid according to claim 1 is recovered system selective breeding method, it is characterized in that, the japonica rice sterile in the said step (4) is Yunnan I type, BT type, seal water type and/or wild abortion type.
10. long grain Japonica Hybrid according to claim 1 is recovered system selective breeding method, it is characterized in that repeating step (4) is further verified its restorative and economical character, obtains the good long grain japonica rice of economical character and recovers system.
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Cited By (9)
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| CN105993930A (en) * | 2016-05-12 | 2016-10-12 | 安徽理想种业有限公司 | Breeding method for long grain type high temperature resistance conventional japonica rice variety |
| CN106489721A (en) * | 2016-10-28 | 2017-03-15 | 福建金山都发展有限公司 | A kind of two-line sterile line selection of the short round shaped grain type of Oryza sativa L. high-quality |
| CN107960319A (en) * | 2017-11-23 | 2018-04-27 | 安徽省绿福农业科技股份有限公司 | A kind of long grain round-grained rice rice variety selective and screening technique |
| CN108770678A (en) * | 2018-04-16 | 2018-11-09 | 陈日胜 | A kind of crossbreeding selection of sea rice and Restoring Line of Japonica Rice |
| CN109287475A (en) * | 2018-12-11 | 2019-02-01 | 中国水稻研究所 | A kind of long grain Japonica Hybrid selection of two systems |
| CN109287478A (en) * | 2018-12-11 | 2019-02-01 | 中国水稻研究所 | A kind of long particle shape two-line sterile line of rice selection |
| CN109349102A (en) * | 2018-12-11 | 2019-02-19 | 中国水稻研究所 | A kind of long grain japonica rice two-line sterile line selection |
| CN112522279A (en) * | 2020-11-19 | 2021-03-19 | 厦门大学 | Coding sequence of rice grain type gene OsGL8 gene and application |
| CN113261500A (en) * | 2021-05-07 | 2021-08-17 | 湖南杂交水稻研究中心 | Breeding method of special ultrahigh-yield hybrid rice for brewing Luzhou-flavor liquor |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105993930A (en) * | 2016-05-12 | 2016-10-12 | 安徽理想种业有限公司 | Breeding method for long grain type high temperature resistance conventional japonica rice variety |
| CN106489721A (en) * | 2016-10-28 | 2017-03-15 | 福建金山都发展有限公司 | A kind of two-line sterile line selection of the short round shaped grain type of Oryza sativa L. high-quality |
| CN107960319A (en) * | 2017-11-23 | 2018-04-27 | 安徽省绿福农业科技股份有限公司 | A kind of long grain round-grained rice rice variety selective and screening technique |
| CN108770678A (en) * | 2018-04-16 | 2018-11-09 | 陈日胜 | A kind of crossbreeding selection of sea rice and Restoring Line of Japonica Rice |
| CN108770678B (en) * | 2018-04-16 | 2021-07-16 | 陈日胜 | Cross breeding and breeding method of sea rice and japonica rice restorer |
| CN109287475A (en) * | 2018-12-11 | 2019-02-01 | 中国水稻研究所 | A kind of long grain Japonica Hybrid selection of two systems |
| CN109287478A (en) * | 2018-12-11 | 2019-02-01 | 中国水稻研究所 | A kind of long particle shape two-line sterile line of rice selection |
| CN109349102A (en) * | 2018-12-11 | 2019-02-19 | 中国水稻研究所 | A kind of long grain japonica rice two-line sterile line selection |
| CN112522279A (en) * | 2020-11-19 | 2021-03-19 | 厦门大学 | Coding sequence of rice grain type gene OsGL8 gene and application |
| CN113261500A (en) * | 2021-05-07 | 2021-08-17 | 湖南杂交水稻研究中心 | Breeding method of special ultrahigh-yield hybrid rice for brewing Luzhou-flavor liquor |
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