CN103026964B

CN103026964B - Method for breeding hybrid rice new material through hybridizing two-line sterile line and three-line maintainer line

Info

Publication number: CN103026964B
Application number: CN201210590479.XA
Authority: CN
Inventors: 陈永军; 胡运高; 杨国涛; 谢崇华
Original assignee: Southwest University of Science and Technology
Current assignee: Southwest Jiaotong University; Southwest University of Science and Technology
Priority date: 2012-12-14
Filing date: 2012-12-14
Publication date: 2014-06-11
Anticipated expiration: 2032-12-14
Also published as: CN103026964A

Abstract

The invention discloses a method for breeding a hybrid rice new material through hybridizing a two-line sterile line and a three-line maintainer line. The method comprises the steps of selecting two-line sterile line Y58S female parent to sexually hybridize with a disease-resistant maintainer line Guangkang 13B, planting F1, planting a large group F2, selecting a single plant in accordance with breeding objectives, using a new mass source sterile line JW803A as a test-crossing female parent to conduct test cross with the excellent single plant, selecting thorough fertility aborted plant line for back cross, allowing the corresponding maintainer line to conduct self-cross, and breeding a new sterile line and a corresponding maintainer line of the new sterile line, which are excellent in quality, disease-resistant, lodging-resistant, stable in fertility, and thoroughly aborted and has the advantages of a two line and a three line, wherein the two-line sterile line Y58S female parent has favorable plant and leaf shapes such as narrow leaf blades, uprightness and smaller leaf angles that facilitate absorption, utilization and the like of light energy; and the breeding objectives are the narrow leaf blades, uprightness, smaller leaf angles and plant characters similar to those of the female parent Y58S.

Description

Utilizing two-line sterile line and three is the method for maintainer selection cross hybrid rice new material

Technical field

What the present invention relates to is that a kind of two-line sterile line and three that utilizes is the method for maintainer selection cross hybrid rice new material.

Background technology

In recent years, particularly the growth momentum of In Middle And Lower Reaches of Changjiang River is powerful in south China rice district for double-linear hybrid rice, its most kinds are mainly manifested in quality better, lodging resistance is strong, the aspects such as high light efficiency, particularly its blade is narrow, uprightly, less absorption and the utilization that waits the leaf state of strain to be more conducive to luminous energy in leaf angle, but the larger two-line sterile line of application area is as Peiai 64S, it is poor that the ubiquities such as Y58S disease resistance, be subject to the impact of soft light reaction characteristic larger, cause it in breeding and production of hybrid seeds process, easily to cause the appearance of the underproduction and high hybrid strain, bring or many losses thereby give to produce in different year, and most three be male sterile line (corresponding is its maintainer) although to show its fertility on the one hand more stable to soft light reaction, breeding, the more high advantage of hybrid seed yield, but its blade is grown up, middle angle is large, lodging resistance is generally poor, thereby affect development and the application of series of three-series hybrid rice kind in the middle and lower reach of Yangtze River.

When seed selection hybrid rice new material (male sterile line, restorer etc.), the majority of prior art is three to be that maintainer and three is that maintainer hybridization, three is that maintainer and other japonica rice or wild rice etc. are hybridized.

Summary of the invention

It is the hybridization of maintainer that the present invention adopts two-line sterile line and three, by pedigree selection, test cross, the mode that backcrosses, what seed selection was new has three of the leaf state of japonica rice strain is male sterile line, thereby for assembly is best in quality, disease resistance is strong, the high-yield hybrid new rice variety of high photoeffect.

Technical scheme of the present invention is as follows:

Select that blade is narrow, upright, leaf angle is less etc. and be conducive to the two-line sterile line Y58S of the favourable leaf state of strain of absorption and the utilization etc. of luminous energy maternal with the wide anti-13B sexual hybridization of disease-resistant maintainer; Plantation F1; Plantation large group F2, selection meets the individual plant of breeding objective, and breeding objective is: blade is narrow, upright, leaf angle is less, and tree characteristics is tended to maternal Y58S; With new germplasm source male sterile line JW803A as test cross maternal with fine individual plant test cross; Selection fertility abortion thoroughly strain backcrosses, corresponding maintainer selfing; Be bred as best in qualityly, disease-resistant, anti-fall, abortion is thorough, and it is to be new sterile line and the corresponding maintainer thereof of advantage with three that stable fertility has two simultaneously.

The present invention is directed to two is to be merits and demerits separately with three, utilization of the present invention is maintainer hybridization to soft light reaction sensitivity and the good two-line sterile line and indica type disease-resistant three of the leaf state of strain, select by pedigree, selfing, the method that backcrosses, successfully select the leaf state of strain tendency two and be but the three hybrid rice new sterile lines that are are all inclined in sterile gene performance, soft light reaction characteristic and disease resistance performance, for the selection of sterile line of hybridized rice provides a kind of new approaches and methods.

The present invention has following characteristics:

1. it is in maintainer and male sterile line that the favourable leaf state of strain such as absorption and utilization that narrow, upright two blades that are, leaf angle is less etc. is conducive to luminous energy is successfully transferred to three, for the hybrid rice variety of cultivating high light efficiency provides material resources.

2. by two high-qualitys that are and three be disease-resistant the two successfully combine, cultivating three of disease-resistant, high-quality is maintainer and corresponding male sterile line thereof, for assembly high-quality, disease-resistant new hybrid rice varieties provide parent resource.

3. utilize three to be the stability of abortion characteristic to soft light reaction, overcome in two line fertilities, exist to soft light reaction tender subject, the new material of the incubation tendency three abortion characteristics that are, for it has created the larger space that utilizes.

4. liang be the combination success that is with three, provide a kind of new method and new way of seed selection hybrid rice new material, for the diversification of Parents In Hybrid Rice material provides new material.

5. two lodging resistances that are being fused to three is in maintainer, successfully cultivate lodging resistance stronger three be male sterile line, provide material source for cultivating the anti-fall new hybrid rice varieties of high yield.

Two-line sterile line (adopt in this patent belong to japonica rice) and three is the hybridization of maintainer (adopt in this patent belong to long-grained nonglutinous rice), actual is interspecific cross between Xian, round-grained rice, the successful combination of the two, for indica-japonica heterosis utilization provides possibility, the new hybrid rice varieties of also more giving prominence to for cultivation yield heterosis on the other hand provides may on the one hand.

Embodiment

Below in conjunction with specific embodiment, the present invention is described in detail.

Spring in 2008, in the blue or green adopted scientific base of Mianyang, Sichuan Xinan Science and Technology Univ. rice research institute with selecting blade narrow, uprightly, blast resisting power that the two-line sterile line Y58S (buying in Sichuan Xike Seeds Co., Ltd.) of the favourable leaf state of strain of absorption and utilization etc. that the less grade in leaf angle is conducive to luminous energy is bred as for maternal and Sanming City Institute of agricultural sciences strong three to be the wide anti-13B of maintainer (buying in Sichuan Xike Seeds Co., Ltd.) carry out sexual hybridization (single cross) for male parent, winter in 2008, at English state, Lingshui, Hainan plantation hybridization F1, spring in 2009, at 400 individual plants of Mianyang, Sichuan blue or green justice plantation F2 colony, (blade is narrow to select wherein to meet breeding objective, uprightly, leaf angle is less, tree characteristics is tended to maternal Y58S) 35 of individual plants, winter in 2009, in English state, Lingshui, Hainan 35 strains of plantation (F3), select self-fertile new germplasm source male sterile line JW803A (buying in Sichuan Xike Seeds Co., Ltd.) as test cross female parent, in these 35 strains, select 30 fine individual plants that wherein meet in 15 elite plant strains of breeding objective to carry out fertility test cross as male parent, collect separately the selfed seed of 30 fine individual plants that test cross uses for plantation next year, spring in 2010, in Mianyang, Sichuan 30 test cross F1 of blue or green justice plantation and corresponding test cross male parent (F4) thereof, test cross male parent is carried out blast resistance identification at Sichuan Pujiang simultaneously, in test cross field, first detect by an unaided eye, stamen performance pollen white in its test cross of discovery F1, slight of stature waits in the obvious elite plant strain of sterile gene, choose its pollen and carry out I-KCI dyeing microscopic examination, pass through microscopy, after choosing dyeing, pollen grain is irregular, slight of stature, pollen abortion thoroughly 10 of tester strains is maternal, its corresponding test cross male parent strain is backcrossing for male parent of resisting through Pujiang blast resistance identification, winter in 2010, in English state, Lingshui, Hainan 10 B1F1 strains of plantation and corresponding maintainer (F5) thereof, adopt above same mode, backcross, blast resistance identification, be bred as to 2012 that to have two be that the leaf state of strain and three is two stable excellent sterile strains and the corresponding maintainer thereof of abortion characteristic simultaneously, one of them is named as 1341A, B, simultaneously through carrying out heterosis, hybrid vigor evaluation from different restorers, first-filial generation kind performance yield heterosis is strong, disease resistance is good, and (2012 with 1341A and mid-early maturity restorer Fuhui 838 and continuous extensive 4948 test crosses of self-fertile restorer, its generation kind output is respectively 552.8 kgs/acre, 568.6 public/mu, more precocious contrast spoke excellent 838 (516.3 kgs/acre) volume increase 6.86% respectively, 6.86%, and contrast precocious 5 days breeding time respectively, 7 days (contrast spoke excellent 838 time of infertility be 139 days), , Mianhui725 extensive 527 with 1341A and slow ripe restorer another name for Sichuan Province, the test crosses such as continuous extensive 4251,623.5 kgs/acre, 637.4 kgs/acre, 645.2 kgs/acre respectively of its generation kind output, contrast respectively (598.7 kgs/acre of Gangyou725s, the slow ripe group of check variety of Sichuan paddy rice) volume increase 4.14%, 6.46%, 7.77%, contrast respectively Gangyou725 (the 148 days time of infertility) breeding time early 2 days, early 1 day, early 3 days.The rice blast resistance of maintainer 1341B is through Pujiang Disease garden identification, 0 grade of leaf pest, 3 grades of neck pest, to test cross generation kind because condition restriction is not made blast resistance identification.

Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.

Claims

One kind to utilize two-line sterile line and three be the method for maintainer selection cross hybrid rice new material, it is characterized in that, select the two-line sterile line Y58S of the leaf state of strain of blade is narrow, upright, leaf angle is less, be conducive to luminous energy absorption and utilization maternal with the wide anti-13B sexual hybridization of disease-resistant maintainer; Plantation F1; Plantation large group F2, selection meets the individual plant of breeding objective, and breeding objective is: blade is narrow, upright, leaf angle is less, and tree characteristics is tended to maternal Y58S; With new germplasm source male sterile line JW803A as test cross maternal with fine individual plant test cross; Selection fertility abortion thoroughly strain backcrosses, corresponding maintainer selfing; Be bred as best in qualityly, disease-resistant, anti-fall, abortion is thorough, and it is to be new sterile line and the corresponding maintainer thereof of advantage with three that stable fertility has two simultaneously.