CN1961646A

CN1961646A - Novel method for constructing rice sporinite-gametophyte dual sterile line

Info

Publication number: CN1961646A
Application number: CNA2006101250442A
Authority: CN
Inventors: 陈建国; 周勇; 居超明; 徐国成
Original assignee: Hubei University
Current assignee: Hubei University
Priority date: 2006-11-16
Filing date: 2006-11-16
Publication date: 2007-05-16
Anticipated expiration: 2026-11-16
Also published as: CN100459849C

Abstract

The invention relates to a method for constructing rice sporinite-gametocyte sterile system, wherein it uses the light-temperature sensitive sterile system of sporinite and the cytoplasm sterile system of gametocyte, to obtain the sterile system with sporinite sterile genes and gametocyte sterile gene. The invention can improve the safety of cross rice seed preparation.

Description

A kind of new method that makes up rice sporinite-gametophyte dual sterile line

Technical field

The present invention relates to the method for the cultivation of hybrid rice, particularly a kind of new method that makes up rice sporinite-gametophyte dual sterile line.

Background technology

Plants male sterility is a plant species can not produce normal flower pesticide, pollen or andro gamete in sexual reproduction process a genetic phenomenon, and it extensively is present in the flowering plant.Paddy rice is a self pollination crop, and its breeding is by the pollen of self oogamete to be pollinated to finish.But in the production of hybrid rice, must go up just might the gain the upper hand crossbreed of strong (output height) of the bigger parent's mating (promptly can not be self-pollination) of diversity ratio with heredity.Therefore must when the production of hybrid seeds, take effective measures again and prevent self-pollination.The discovery of male sterile material and be utilized as rice heterosis utilization the otherwise effective technique assurance is provided.Because it can prevent to carry out self-pollination as maternal rice varieties effectively, guarantee that itself and male parent hybridize.This is the key factor that hybrid rice is achieved success.

Can be divided into sterile two types of the sterile and gametophyte of sporophyte to male sterile by the alternation of generations.The sterile sterility of sporophyte is decided by the genotype of plant, as long as the genotype of plant is sterile, all pollen that it produced all are sterile.The sterile sterility of gametophyte is decided by the genotype of andro gamete (pollen), has only the andro gamete (pollen) that itself has sterile gene to be only sterile.The two looks like a pair of contradiction, both can't be unified in the same individuality.Therefore never the someone attempted cultivating and not only has the sporophyte sterile gene, but also has the dual sterile line of gametophyte sterile gene.Also nobody studies it on method.

Summary of the invention

The present invention proposes a kind of belonging to the sterile optical-and thermal-sensitive type caryon sterile line of sporophyte and belonging to two kinds of male sterile materials of the sterile cytoplasmic male sterile line of gametophyte in the paddy rice of utilizing, and makes up not only to have the sporophyte sterile gene, but also have the breeding method of hybrid rice of the dual sterile line of gametophyte sterile gene.

The present invention is achieved by the following technical solutions:

A. earlier maintenance line is become to have the two-line sterile line N (rfrf ss) of gametophyte sterile gene by the continuous backcross transformation.Use up, temperature-sensitive sterile line is done the maternal maintenance line hybridization sterile with gametophyte, the F1 selfing obtains F2, in F2, select temperature sensitive sterile individual plant of light and maintenance line to backcross and obtain BC1, the BC1 selfing obtains BC1F2, in BC1F2, select temperature sensitive sterile individual plant of light and maintenance line to backcross and obtain BC2, the BC2 selfing obtains BC2F2, in BC2F2, select temperature sensitive sterile individual plant of light and maintenance line to backcross and obtain BC3, ..., repeat the process of above-mentioned " selfing-backcross-selfing-backcross ", backcross through the 4-8 wheel, obtain BC4-8, the BC4-8 selfing obtains BC4-8F2, the temperature sensitive sterile individual plant of the light of selecting in BC4-8F2 is the two-line sterile line that has the gametophyte sterile gene, this two-line sterile line was on long, show as male sterile under the hot conditions, can be used as male sterile line and use, on short, show as male-fertile under the cryogenic conditions again, gamete build cytoplasmic male sterile line had the maintenance effect, here be called " new maintenance line " for the time being, it is as broad as long substantially with former maintenance line except having the temperature sensitive sterile gene of light.

B. use gamete build cytoplasmic male sterile line S (rfrf SS) to do maternal and " new maintenance line " hybridization, F1 backcrosses with " new maintenance line " again, female parent and the paired test cross of photosensitive and temperature-sensitive sterile line that has the gametophyte sterile restoring gene are made in the menu strain in first backcross generation colony, under long day condition, test cross offspring's fertility is investigated, the individual plant that its test cross offspring did not have fertility to separate on long under the condition is exactly not only to have had the sporophyte sterile gene, but also have the dual sterile line of gametophyte sterile gene.

Technology path:

1, maintenance line is become two-line sterile line by the continuous backcross transformation:

Photo-thermo-sensitive genic male sterile line * maintainer → F1 → (selfing) F2 → the select temperature sensitive sterile individual plant * maintainer of light → BC1 → (selfing) BC1F2 → the select temperature sensitive sterile individual plant * maintainer of light → BC2 → (selfing) BC2F2 → the select temperature sensitive sterile individual plant * maintainer of light → BC3 → (selfing) → BC3F2 → the select temperature sensitive sterile individual plant * maintainer of light → BC4 → (selfing) BC4F2 → the select temperature sensitive sterile individual plant * maintainer of light → BC5 → (selfing) BC5F2 → the select temperature sensitive sterile individual plant * maintainer of light → BC6 → (selfing) BC6F2 → under long day condition BC6F2 colony is carried out Fertility identification, the individual plant with the temperature sensitive sterile gene of light is new maintainer.

2, the seed selection of dual sterile line:

Gametophyte male sterile line * new maintenance line → F1 → (backcrossing) BC1 → menu strain and the paired test cross of the photosensitive and temperature-sensitive sterile line → test cross offspring who has the gametophyte sterile restoring gene with new maintenance line → under long day condition, test cross offspring's fertility is investigated, the individual plant that its test cross offspring did not have fertility to separate on long under the condition is exactly not only to have had the sporophyte sterile gene, but also have the dual sterile line of gametophyte sterile gene.

Advantage of the present invention

Dual sterile line of the present invention, sterile and the gametophyte sterility of its sporophyte can be replenished mutually under long day, hot conditions, make the fertility abortion more thorough, reduce greatly, improve the safety of breeding of hybrid rice owing to the not thorough production of hybrid seeds risk that causes of fertility abortion.

Dual sterile line of the present invention, have sporophyte sterile gene and gametophyte sterile gene simultaneously, the both can express under long day, hot conditions, and under short day, cryogenic conditions, have only the gametophyte sterile gene that sterility is worked, thereby be the interactional ideal material of research sporophyte sterile gene and gametophyte sterile gene.

This method has broken through theoretically can not be with the sterile and sterile difficult problem that is integrated in one of gametophyte of sporophyte, on using, two kinds of sterility can be complementary, efficiently solves responsive to temperature type caryon sterile line temperature influence and occur fertility fluctuation (being commonly called as " malaria ") and the halfway problem of gametophyte male sterile line fertility abortion easily.Therefore breeding of this material all is significant for the practical application of in same genetic background two kinds of dissimilar male sterilities being carried out research of fertility mechanism and hybrid rice.

Embodiment:

The invention will be further described with embodiment below:

Embodiment 1: with light, the fragrant 125S of temperature-sensitive sterile line (sporophyte is sterile) does maternal, safe B makes male parent with the maintenance line Guangdong of the safe A in gamete build cytoplasmic male sterile line Guangdong, hybridization obtains F1, the F1 selfing obtains F2, in F2, select the safe B in temperature sensitive sterile individual plant of light and Guangdong to backcross and obtain BC1, the BC1 selfing obtains BC1F2, in BC1F2, select the safe B in temperature sensitive sterile individual plant of light and Guangdong to backcross and obtain BC2, the BC2 selfing obtains BC2F2, in BC2F2, select the safe B in temperature sensitive sterile individual plant of light and Guangdong to backcross and obtain BC3, ..., repeat the process of above-mentioned " selfing-backcross-selfing-backcross ", take turns through 4 and backcross, obtain BC4, the BC4 selfing obtains BC4F2, and the temperature sensitive sterile individual plant of the light of selecting in BC4F2 is the two-line sterile line that has the gametophyte sterile gene, is designated as the safe Bs in Guangdong.Do maternal and the safe Bs hybridization in Guangdong with the safe A in Guangdong, F1 backcrosses with the safe Bs in Guangdong again, the menu strain is done maternal and the paired test cross of photosensitive and temperature-sensitive sterile line 9802s (the recovery gene that has the safe A in Guangdong) in first backcross generation colony, under long day, hot conditions, test cross offspring's fertility is investigated, the individual plant that its test cross offspring does not have fertility to separate under long day, hot conditions, be exactly not only to have had the sporophyte sterile gene, but also have the dual sterile line of gametophyte sterile gene, be designated as the safe As in Guangdong.

Embodiment 2: with light, temperature-sensitive sterile line strain 1S (sporophyte is sterile) does maternal, maintenance line Cong Guang 41B with gamete build cytoplasmic male sterile line Cong Guang 41A makes male parent, hybridization obtains F1, the F1 selfing obtains F2, in F2, select temperature sensitive sterile individual plant of light and Cong Guang 41B to backcross and obtain BC1, the BC1 selfing obtains BC1F2, in BC1F2, select temperature sensitive sterile individual plant of light and Cong Guang 41B to backcross and obtain BC2, the BC2 selfing obtains BC2F2, in BC2F2, select temperature sensitive sterile individual plant of light and Cong Guang 41B to backcross and obtain BC3, ..., repeat the process of above-mentioned " selfing-backcross-selfing-backcross ", take turns through 8 and backcross, obtain BC8, the BC8 selfing obtains BC8F2, and the temperature sensitive sterile individual plant of the light of selecting in BC8F2 is the two-line sterile line that has the gametophyte sterile gene, is designated as Cong Guang 41Bs.Do maternal and Cong Guang 41bs hybridization with Cong Guang 41A, F1 backcrosses with Cong Guang 41Bs again, the menu strain is done maternal and the paired test cross of indica type photosensitive-sensitive sterile line 1103s (the recovery gene that has Cong Guang 41A) in first backcross generation colony, under long day, hot conditions, test cross offspring's fertility is investigated, the individual plant that its test cross offspring does not have fertility to separate under long day, hot conditions, be exactly not only to have had the sporophyte sterile gene, but also have the dual sterile line of gametophyte sterile gene, be designated as Cong Guang 41As.

Claims

1, a kind of new method of structure rice sporinite-gametophyte dual sterile line is characterized in that:

A, maintenance line maternal with the photosensitive and temperature-sensitive sterile line work and that gametophyte is sterile are hybridized, process by 4-8 wheel " selfing-backcross-selfing-backcross ", earlier maintenance line is become to have the two-line sterile line of gametophyte sterile gene by the continuous backcross transformation, this two-line sterile line shows as male sterile under long day, hot conditions, can be used as male sterile line uses, under short day, cryogenic conditions, show as male-fertile again, gamete build cytoplasmic male sterile line is had the maintenance effect, be called " new maintenance line ";

B, gamete build cytoplasmic male sterile line are done maternal and " new maintenance line " hybridization, F1 backcrosses with " new maintenance line " again, female parent and the paired test cross of photosensitive and temperature-sensitive sterile line that has the gametophyte sterile restoring gene are made in the menu strain in first backcross generation colony, under long day condition, test cross offspring's fertility is investigated, the individual plant that its test cross offspring did not have fertility to separate on long under the condition is exactly not only to have had the sporophyte sterile gene, but also have the dual sterile line of gametophyte sterile gene.