CN103733978A - Method for breeding male sterile line by use of sterile cytoplasm of hibiscus cannabinus KN250 - Google Patents
Method for breeding male sterile line by use of sterile cytoplasm of hibiscus cannabinus KN250 Download PDFInfo
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- CN103733978A CN103733978A CN201310714202.8A CN201310714202A CN103733978A CN 103733978 A CN103733978 A CN 103733978A CN 201310714202 A CN201310714202 A CN 201310714202A CN 103733978 A CN103733978 A CN 103733978A
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Abstract
The invention discloses a method for breeding a male sterile line by use of the sterile cytoplasm of hibiscus cannabinus KN250, which specifically comprises the following steps: performing saturation backcross by taking a breeding material containing the sterile cytoplasm of hibiscus cannabinus KN250 as a female parent and the cultivated variety of hibiscus cannabinus as a male parent, and selecting a uniform wild abortive type cytoplasmic male sterile line similar to the male parent in character and showing male sterility, wherein the male parent for backcross is a corresponding male sterility maintainer line. The method disclosed by the invention has the beneficial effects that the hybrid matched with the KN250 cytoplasmic male sterile line and the hybrid matched with the UG93 or H040 cytoplasmic male sterile line are popularized and utilized at the same time, and the occurrence risk of certain disease can be greatly reduced.
Description
Technical field
The present invention relates to crop breeding method, specifically utilize the method for the sterile cytoplasm seed selection male sterile line of bluish dogbane KN250.
Background technology
Bluish dogbane claims again " mestha ", is the annual phloem fiber crop of Malvaceae Hibiscus.Because bluish dogbane is to gather in the crops phloem fiber or stem stalk as cultivation object, heterosis rate is up to 35%～40%, and bluish dogbane heterosis utilization is the main goal of attack of domestic and international bluish dogbane breeding for a long time.Hemp Inst., China Academy of Agricultural Sciences, through research for many years, has invented bluish dogbane chemical emasculation cross breeding method, and has applied in bluish dogbane breeding.But because bluish dogbane is indefinite inflorescence, need repeatedly dispenser, and after dispenser, affected insect pollination, need artificial pollination.For cost-saving, have to promote aborning F2 generation (second filial generation).But F2 is irregular for plant, heterosis rate obviously reduces, therefore the Kenaf Hybrid H area of promoting in bluish dogbane produces is so far very limited.Therefore, seed selection bluish dogbane cytoplasmic male sterile line is to utilize heterotic key problem in technology on bluish dogbane produces.
In September, 2008, " selection of bluish dogbane Yebai cytoplasmic male sterile line " of the Zhou Ruiyang of Guangxi University invention obtains national inventing patent (ZL200510019454.4, publication number CN100415083C).It is characterized in that there is the cytoplasmic filial generation of bluish dogbane wild species UG93 as maternal take bluish dogbane wild species UG93 or heredity, take bluish dogbane cultivar as male parent, carry out saturated backcrossing, therefrom select proterties similar to male parent, neat and consistent, and show male sterile Yebai cytoplasmic male sterile line, its male parent used that backcrosses is male sterility maintainer line.The male sterile line of seed selection and crossbreed thereof are UG93 cytoplasm thus.
On December 5th, 2012, " selection of bluish dogbane H040 cytoplasmic male sterile line " of the Zhou Ruiyang of Guangxi University invention obtains national inventing patent (ZL200810073873.X).It is characterized in that there is the cytoplasmic filial generation of bluish dogbane wild species H040 as maternal take bluish dogbane wild species H040 or heredity, take bluish dogbane cultivar as male parent, carry out saturated backcrossing, therefrom select proterties similar to male parent, neat and consistent, and show male sterile Yebai cytoplasmic male sterile line, its male parent used that backcrosses is male sterility maintainer line.The male sterile line of seed selection and crossbreed thereof are H040 cytoplasm thus.
This area similarly invention also had: Yuan Longping etc.s, take the natural abortion mutant found in wild rice as female parent, by the method for continuous backcross, selected the cytoplasmic male sterile lines such as precious Xian 97A in 1973.1980, hybrid rice transferred the U.S. as first agrotechnique patent of China.But as far back as nineteen sixty-eight, limit is crossed with No. 5 hybridization of Burma Reed rice (Lead rice) and rattan slope by Japanese agriculture technical research institute, pass through continuous backcross, be bred as and there are No. 5 male sterile lines of the cytoplasmic rattan slope of Reed and (seen: the chief editor of the Chinese Academy of Agricultural Sciences, < < China rice makes to learn the 331st page～334 page the 2nd row of > >, agricultural publishing house, 1986), early than Mr. Yuan Longping.
But in agricultural production, there is the pandemic risk of a certain disease in spread and long-term utilization by the crossbreed of single cell matter male sterile line assembly, because the crossbreed of this cytoplasmic male sterile line assembly may become the nurture kind of a certain pathogenic microorganism biological strain.The Basic Ways addressing this problem is the male sterile line that selects various kinds of cell matter source, and promotes aborning the three line hybrid seed in different cytoplasm source.
Summary of the invention
The object of the invention is to solve the problem in bluish dogbane crossbreed single cell matter source, a kind of selection of novel bluish dogbane cytoplasmic male sterile line is provided, utilize the method for the sterile cytoplasm seed selection male sterile line of bluish dogbane KN250.
KN250 was the bluish dogbane wild species of Kenya, by Hemp Inst., China Academy of Agricultural Sciences, was provided.Inventor finds that by Molecular Detection the cytoplasm of the bluish dogbane wild species KN250 that can educate is sterile cytoplasm, then plant the seed of the spontaneous pollination of gathering on this product plant so that hybridization transformation, the individual plant performance male sterile that after plantation wherein there is discovery, this seed selection that is male sterile line provides great convenience.
The present invention is with 1) bluish dogbane wild species KN250,2) the sterile strain that KN250 seminal propagation offspring obtains, or 3) to have the cytoplasmic hybridization of bluish dogbane wild species KN250 or backcross progeny be cytoplasm donor in heredity, take common bluish dogbane cultivar (being) as cell nucleus donor, consequent nucleo-cytoplasmic interaction causes male sterile again.
The method that the present invention is to provide the sterile cytoplasm seed selection male sterile line that utilizes bluish dogbane KN250, concrete steps are as follows:
Take the breeding material that contains bluish dogbane KN250 sterile cytoplasm as maternal, take bluish dogbane cultivar as male parent, carry out saturated backcrossing, therefrom select proterties similar to male parent, neat and consistent, and show male sterile Yebai cytoplasmic male sterile line; Its male parent used that backcrosses is corresponding male sterility maintainer line.
Wherein, the breeding material that contains bluish dogbane KN250 sterile cytoplasm described in is one of following 3 kinds of breeding materials:
1) bluish dogbane wild species KN250;
2) the sterile strain of KN250 seminal propagation offspring gained;
3) heredity has hybridization or the backcross progeny of KN250 sterile cytoplasm.
Wherein, when backcrossing described in carrying out, measure coordinate force with multiple cultivars, select that coordinate force is high, proterties is similar to male parent each generation, and the indehiscent male sterile plant of flower pesticide continues to backcross, while male parent selfing.
Wherein, the algebraically backcrossing described in is more than 5 generations (containing).
Wherein, described coordinate force is high refers to that coordinate force is more than mean value, measures the concrete grammar of coordinate force with reference to < < plant breeding > >.
The application of the method that the present invention also provides the sterile cytoplasm seed selection male sterile line that utilizes bluish dogbane KN250 in bluish dogbane breeding.
The application of the male sterile line that method is educated that the present invention further provides the sterile cytoplasm seed selection male sterile line that utilizes bluish dogbane KN250 in bluish dogbane breeding.
Method of the present invention, has the following advantages:
Bluish dogbane wild species KN250 cytoplasmic male sterile line and other bluish dogbane cultivar natural crossing combining go out cross combination which has strong superior, and breeding cost is low.By the crossbreed of the type cytoplasmic male sterile line assembly and the crossbreed of UG93 or the assembly of H040 cytoplasmic male sterile line while utilization and extention aborning, can greatly reduce the risk of the large generation of certain disease.
Embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
Embodiment 1 is with bluish dogbane wild species KN250 transformation male sterile line
Take bluish dogbane wild species KN250 as maternal, take Guangdong, rich No. 1 B(is shown in: national inventing patent ZL200510019454.4 embodiment 3) be male parent, adopt the saturated method backcrossing, selected male sterile line K5P3A and the maintainer K5P3B thereof with KN250 male sterile cytoplasm.
Specific as follows:
2008-2010, take KN250 as maternal, take Guangdong, rich No. 1 B is paternal hybrid, take Guangdong, rich No. 1 B backcrosses as male parent afterwards, when backcrossing, and take good fortune red 992 and R1 as paternal hybrid, measure coordinate force, select each that coordinate force is high, proterties is similar to male parent from generation to generation, and the indehiscent male sterile plant of flower pesticide continues to backcross, while male parent selfing.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, obtain K5P3A, the Characters is similar to rich No. 1 B in Guangdong, and fertility shows as male sterile.Its maintainer is K5P3B.
Embodiment 2 is with the sterile strain transformation of KN250 seminal propagation offspring gained male sterile line
The seed of results bluish dogbane wild species KN250 spontaneous pollination, plantation obtains seminal propagation offspring, and therefrom screening obtains sterile strain.Take the sterile strain of gained as maternal, take Guangdong, rich No. 1 B(is shown in: national inventing patent ZL200510019454.4 embodiment 3) be male parent, adopt the saturated method backcrossing, selected male sterile line K5TP3A and the maintainer K5TP3B thereof with KN250 male sterile cytoplasm.
Specific as follows:
2010-2012, take the male sterile plant found in KN250 seminal propagation offspring as maternal, take Guangdong, rich No. 1 B is paternal hybrid, take Guangdong, rich No. 1 B backcrosses as male parent afterwards, when backcrossing, and take good fortune red 992 and R1 as paternal hybrid, measure coordinate force, select each that coordinate force is high, proterties is similar to male parent from generation to generation, and the indehiscent male sterile plant of flower pesticide continues to backcross, while male parent bagging.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, obtain K5TP3A, the Characters is similar to rich No. 1 B in Guangdong, and fertility shows as male sterile.Its maintainer is K5TP3B.
Embodiment 3 is with bluish dogbane wild species KN250 transformation male sterile line
Take bluish dogbane wild species KN250 as maternal, take bluish dogbane conventional cultivation kind golden light stingless (University Of Agriculture and Forestry In Fujian's introduction) as male parent, adopt the saturated method backcrossing, select and there is the cytoplasmic male sterile line K5K3A of KN250 male sterile line and maintainer K5K3B thereof.
Specific as follows:
2010-2012, take KN250 as maternal, stingless as paternal hybrid take golden light, so that golden light is stingless, backcross as male parent afterwards, when backcrossing, take good fortune red 992 and R1 as paternal hybrid, measure coordinate force, each generation selection coordinate force is high, proterties is similar to male parent, and the indehiscent male sterile plant of flower pesticide continues to backcross, male parent selfing simultaneously, in Nanning and Lingshui, Hainan, backcross every year, in 6 generations that backcrossed altogether for 3 years, obtain K5K3A, the Characters is stingless similar to golden light, and fertility shows as male sterile.Its maintainer is K5K3B.
Embodiment 4 is with the sterile strain transformation of KN250 seminal propagation offspring gained male sterile line
The seed of results bluish dogbane wild species KN250 spontaneous pollination, plantation obtains seminal propagation offspring, and therefrom screening obtains sterile strain.Take the sterile strain of gained as maternal, take bluish dogbane conventional cultivation kind golden light stingless (University Of Agriculture and Forestry In Fujian's introduction) as male parent, adopt the saturated method backcrossing, select and there is the cytoplasmic male sterile line K5TK3A of KN250 male sterile line and maintainer K5TK3B thereof.
Specific as follows:
2010-2012, take the male sterile plant found in KN250 seminal propagation offspring as maternal, stingless as paternal hybrid take golden light, so that golden light is stingless, backcross as male parent afterwards, when backcrossing, take good fortune red 992 and R1 as paternal hybrid, measure coordinate force, each generation selects coordinate force high, proterties is similar to male parent, and the indehiscent male sterile plant of flower pesticide continues to backcross, male parent selfing simultaneously, in Nanning and Lingshui, Hainan, backcross every year, in 6 generations, backcrossed for 3 years altogether, obtain K5TK3A, the Characters is stingless similar to golden light, fertility shows as male sterile.Its maintainer is K5TK3B.
Embodiment 5 has the filial generation transformation male sterile line of KN250 sterile cytoplasm with heredity
Take the first-filial generation of rich No. 1 B in bluish dogbane wild species KN250 × Guangdong as maternal, with bluish dogbane cultivated species good fortune, (have another name called F3A red No. 3, see patent of invention ZL200510019454.4, publication number CN100415083C, embodiment 4) be male parent, adopt the saturated method backcrossing, select male sterile line K5F3A and the maintainer K5F3B thereof with KN250 sterile cytoplasm.
Specific as follows:
2011-2013, take the first-filial generation of rich No. 1 B in bluish dogbane wild species KN250 × Guangdong as maternal, take good fortune red No. 3 as paternal hybrid, take good fortune, backcross as male parent for red No. 3 afterwards, when backcrossing, take good fortune red 992 and R1 as paternal hybrid, measure coordinate force, select each that coordinate force is high, proterties is similar to male parent from generation to generation, and the indehiscent male sterile plant of flower pesticide continues to backcross, while male parent selfing.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, obtain K5F3A, the Characters and good fortune red No. 3 similar, fertility shows as male sterile.Its maintainer is K5F3B.
Embodiment 6 has the filial generation transformation male sterile line of KN250 sterile cytoplasm with heredity
Take the first-filial generation of bluish dogbane wild species KN250 seminal propagation offspring's gained rich No. 1 B in sterile strain × Guangdong as maternal, with bluish dogbane cultivated species good fortune, (have another name called F3A red No. 3, see patent of invention ZL200510019454.4, publication number CN100415083C, embodiment 4) be male parent, adopt the saturated method backcrossing, select male sterile line K5TF3A and the maintainer K5TF3B thereof with KN250 sterile cytoplasm.
Specific as follows:
2011-2013, take the first-filial generation of bluish dogbane wild species KN250 seminal propagation offspring's gained rich No. 1 B in sterile strain × Guangdong as maternal, take good fortune red No. 3 as paternal hybrid, take good fortune, backcross as male parent for red No. 3 afterwards, when backcrossing, take good fortune red 992 and R1 as paternal hybrid, measure coordinate force, select each that coordinate force is high, proterties is similar to male parent from generation to generation, and the indehiscent male sterile plant of flower pesticide continues to backcross, while male parent selfing.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, obtain K5TF3A, the Characters and good fortune red No. 3 similar, fertility shows as male sterile.Its maintainer is K5TF3B.
Embodiment 7 is with the sterile cytoplasm transformation male sterile line of the saturated gained K5P3A that backcrosses of embodiment 1
Take the saturated gained K5P3A that backcrosses of embodiment 1 as maternal, take T17(Hemp Inst., China Academy of Agricultural Sciences, introduce) as male parent, adopt the saturated method backcrossing, selected male sterile line K5T17A and the maintainer K5T17B thereof with KN250 male sterile cytoplasm.
Specific as follows:
2011-2013, take the saturated gained K5P3A that backcrosses of embodiment 1 as maternal, take T17(Hemp Inst., China Academy of Agricultural Sciences, introduce) as male parent, take T17 as male parent, backcross afterwards, when backcrossing, take good fortune red 992 and R1 as paternal hybrid, measure coordinate force, select each that coordinate force is high, proterties is similar to male parent from generation to generation, and the indehiscent male sterile plant of flower pesticide continues to backcross, while male parent selfing.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, obtain K5T17A, the Characters is similar to T17, and fertility shows as male sterile.Its maintainer is K5T17B.
Embodiment 8 has the filial generation transformation male sterile line of KN250 sterile cytoplasm with heredity
Take the stingless first-filial generation of bluish dogbane wild species KN250 × golden light as maternal, with bluish dogbane cultivated species, 917(has another name called 917B, the Kenaf Cultivars of Hemp Inst., China Academy of Agricultural Sciences's seed selection) be male parent, adopt the saturated method backcrossing, selected male sterile line K5917A and the maintainer K5917B thereof with KN250 male sterile cytoplasm.
2011-2013, take the stingless first-filial generation of bluish dogbane wild species KN250 × golden light as maternal, take 917 as paternal hybrid, take 917 as male parent, backcross afterwards, when backcrossing, take good fortune red 992 and R1 as paternal hybrid, measure coordinate force, select each that coordinate force is high, proterties is similar to male parent from generation to generation, and the indehiscent male sterile plant of flower pesticide continues to backcross, while male parent selfing.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, must obtain K5917A, the Characters is similar to 917, and fertility shows as male sterile.Its maintainer is K5917B.
Embodiment 9 has the filial generation transformation male sterile line of KN250 sterile cytoplasm with heredity
Take the stingless first-filial generation of the sterile strain × golden light of bluish dogbane wild species KN250 seminal propagation offspring's gained as maternal, with bluish dogbane cultivated species, 917(has another name called 917B, the Kenaf Cultivars of Hemp Inst., China Academy of Agricultural Sciences's seed selection) be male parent, adopt the saturated method backcrossing, selected male sterile line K5T917A and the maintainer K5T917B thereof with KN250 male sterile cytoplasm.
Specific as follows:
2011-2013, take the stingless first-filial generation of the sterile strain × golden light of bluish dogbane wild species KN250 seminal propagation offspring's gained as maternal, take 917 as paternal hybrid, take 917 as male parent, backcross afterwards, when backcrossing, take good fortune red 992 and R1 as paternal hybrid, measure coordinate force, select each that coordinate force is high, proterties is similar to male parent from generation to generation, and the indehiscent male sterile plant of flower pesticide continues to backcross, while male parent selfing.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, obtain K5T917A, the Characters is similar to 917, and fertility shows as male sterile.Its maintainer is K5T917B.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (6)
1. the method for utilizing the sterile cytoplasm seed selection male sterile line of bluish dogbane KN250, concrete steps are as follows:
Take the breeding material that contains bluish dogbane KN250 sterile cytoplasm as maternal, take bluish dogbane cultivar as male parent, carry out saturated backcrossing, therefrom select proterties similar to male parent, neat and consistent, and show male sterile Yebai cytoplasmic male sterile line; Its male parent used that backcrosses is corresponding male sterility maintainer line.
2. method according to claim 1, is characterized in that, described in contain bluish dogbane KN250 sterile cytoplasm breeding material be one of following 3 kinds of breeding materials:
1) bluish dogbane wild species KN250;
2) the sterile strain of KN250 seminal propagation offspring gained;
3) heredity has hybridization or the backcross progeny of KN250 sterile cytoplasm.
3. according to the method described in claim 1-2 any one, it is characterized in that, when backcrossing described in carrying out, measure coordinate force with multiple cultivars, select each that coordinate force is high, proterties is similar to male parent from generation to generation, and the indehiscent male sterile plant of flower pesticide continues to backcross, while male parent selfing.
4. according to the method described in claim 1-2 any one, it is characterized in that, described in the algebraically that backcrosses be more than 5 generations (containing).
5. the application of the method for sterile cytoplasm seed selection male sterile line of utilizing bluish dogbane KN250 described in claim 1-4 any one in bluish dogbane breeding.
6. the application in bluish dogbane breeding according to the male sterile line that utilizes the method seed selection of sterile cytoplasm seed selection male sterile line of bluish dogbane KN250 to obtain described in claim 1-4 any one.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104620978A (en) * | 2015-02-10 | 2015-05-20 | 福建农林大学 | Breeding method of cytoplasmic male sterile line of quality disease-resistant photo periodical insensitive hibiscus cannabinus |
| CN104798679A (en) * | 2015-05-12 | 2015-07-29 | 中国农业科学院麻类研究所 | Breeding method of light insensitive kenaf male sterility line 261N5-18A |
| CN106755081A (en) * | 2017-01-25 | 2017-05-31 | 广西大学 | A kind of method that cotton transgenic formulates cytoplasmic male sterile line |
| CN107996388A (en) * | 2017-12-04 | 2018-05-08 | 广西大学 | A kind of method using freestone kapok initiative cotton cells matter male sterile line |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1748469A (en) * | 2005-09-17 | 2006-03-22 | 广西大学 | Breeding method for bluish dogbane Yebai cytoplasmic male sterile line |
| CN101401540A (en) * | 2008-11-03 | 2009-04-08 | 广西大学 | Hibiscus cannabinus l. H040 cytoplasmic male sterility phyletic breeding method |
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2013
- 2013-12-19 CN CN201310714202.8A patent/CN103733978B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1748469A (en) * | 2005-09-17 | 2006-03-22 | 广西大学 | Breeding method for bluish dogbane Yebai cytoplasmic male sterile line |
| CN101401540A (en) * | 2008-11-03 | 2009-04-08 | 广西大学 | Hibiscus cannabinus l. H040 cytoplasmic male sterility phyletic breeding method |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104620978A (en) * | 2015-02-10 | 2015-05-20 | 福建农林大学 | Breeding method of cytoplasmic male sterile line of quality disease-resistant photo periodical insensitive hibiscus cannabinus |
| CN104798679A (en) * | 2015-05-12 | 2015-07-29 | 中国农业科学院麻类研究所 | Breeding method of light insensitive kenaf male sterility line 261N5-18A |
| CN104798679B (en) * | 2015-05-12 | 2017-04-12 | 中国农业科学院麻类研究所 | Breeding method of light insensitive kenaf male sterility line 261N5-18A |
| CN106755081A (en) * | 2017-01-25 | 2017-05-31 | 广西大学 | A kind of method that cotton transgenic formulates cytoplasmic male sterile line |
| CN106755081B (en) * | 2017-01-25 | 2020-06-16 | 广西大学 | Method for creating cytoplasmic male sterile line by cotton transgenosis |
| CN107996388A (en) * | 2017-12-04 | 2018-05-08 | 广西大学 | A kind of method using freestone kapok initiative cotton cells matter male sterile line |
| CN107996388B (en) * | 2017-12-04 | 2021-03-30 | 广西大学 | Method for creating cotton cytoplasmic male sterile line by using karyorrhoea batal |
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| CN103733978B (en) | 2015-09-16 |
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