CN104335894A - Selective breeding method of cytoplasmic male sterility line of eggplant resisting to greensickness and blight - Google Patents
Selective breeding method of cytoplasmic male sterility line of eggplant resisting to greensickness and blight Download PDFInfo
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- CN104335894A CN104335894A CN201410638018.4A CN201410638018A CN104335894A CN 104335894 A CN104335894 A CN 104335894A CN 201410638018 A CN201410638018 A CN 201410638018A CN 104335894 A CN104335894 A CN 104335894A
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Abstract
The invention relates to a selective breeding method of a cytoplasmic male sterility line of an eggplant resisting to greensickness and blight, and belongs to the technical field of plant breeding. The method is characterized in that blight-resisting male sterile plants of filial generations between African red eggplant (Solanum aethiopicum group Aculeatum) and 'Seven-leaf Eggplants' are used as female parents; a single greensickness-resisting plant of filial generations between wild eggplants (S. linnaeanum) and 'Suzhou Ox Horns' is used as a male parent; the female parents and the male parent are hybridized; the male sterile plants resisting to the greensickness and the blight are selected from the filial generations; five generations are continuously backcrossed by taking the 'Suzhou Ox Horns' as the male parents; the male sterility line 'SaetC1' is selectively bred by observing and identifying fertility, resistance to the greensickness, resistance to the blight, and horticultural characters; the male sterility line 'SaetC1' belongs to a no-pollen type sterile line and has the advantages of 100% sterile plant rate, early maturation, 10-11 early flowering nodes, bar-shaped fruits with 25-30cm longitudinal diameter and transverse diameter of 5.0-6.0cm of, black purple commodity pericarp, resistance to the greensickness and the blight, and use as the female parents of a selectively-bred eggplant hybrid variety.
Description
one, technical field
The present invention relates to the selection of a kind of resisting verticillium and fusarium wilt eggplant cytoplasm male sterility line, belong to field of plant variety breeding technology, be exclusively used in the seed selection of resisting verticillium and fusarium wilt eggplant cytoplasm male sterility line.
two, background technology
Eggplant (
solanum melongena) be a kind of important vegetable crop of China.Eggplant verticillium wilt and fusarium wilt are the 2 kinds of important soil-borne diseases caused by verticillium dahliae and Fusarium oxysporum respectively.In recent years, the occurrence and harm of these 2 kinds of diseases is further serious, and becomes the important disease of eggplant facility cultivation, very urgent to the demand of resisting verticillium and fusarium wilt Eggplant Varieties in production.
Cytoplasmic male sterilty (cytoplasmic male sterility, CMS) extensively exists in higher plant, in plant hybrid use of advantage, have important effect.In the Hybrid seed produces of self pollination crop, utilize male sterile line not only can save artificial emasculation cost, improve the purity of seed, and for preventing parent to run off, protecting the intellectual property has important effect.
Research shows, distant hybridization is the effective means of initiative male sterile line.In earlier stage, we are finding male sterile plant to cultivating in eggplant to import in the Interspecific Hybrids colony of African red eggplant anti-blight gene, backcross with resisting verticillium breeding material and excellent purple long eggplant inbred line and sterile strain further, to selecting resisting verticillium and fusarium wilt eggplant cytoplasm male sterility line, for New Eggplant Varieties seed selection lays the foundation.
three, summary of the invention
technical problem
The object of the invention is to overcome during current eggplant hybrid seed is produced needs artificial emasculation and existing kind to verticillium wilt and the weak deficiency of fusarium wilt disease resistance, provides a kind of selection of eggplant cytoplasm male sterility line.The male sterile line of seed selection does not produce pollen by this method, and sterile plant rate is high, and has the good characteristic of resisting verticillium and fusarium wilt, can be used as the female parent of seed selection eggplant Hybrid.
technical scheme
The male-sterile line breeding method that the present invention provides is the selection of male sterile, resistance to verticillium wilt, the hybridization polymerization of fusarium wilt disease resistance and excellent Horticultural Characters, backcross improvement and offspring thereof.
A selection for resisting verticillium and fusarium wilt eggplant cytoplasm male sterility line, comprising:
With African red eggplant (
solanum aethiopicumgroup
aculeatum)be maternal with the anti-blight male sterile plant in " seven leaf eggplants " Interspecific Hybrids, with wildness eggplant (
s. linnaeanum) be that male parent is hybridized with resisting verticillium individual plant in " Suzhou ox horn " Interspecific Hybrids, filial generation colony is carried out to the qualification of fertility, resistance to verticillium wilt and fusarium wilt disease resistance, screening obtains the male sterile plant of anti-blight and verticillium wilt two kinds of diseases.
To screen the anti-blight and the male sterile plant of verticillium wilt two kinds of diseases that obtain, with " Suzhou ox horn " for male parent backcrosses, each backcross generations colony is carried out to the qualification of fertility, resistance to verticillium wilt, fusarium wilt disease resistance and Horticultural Characters, through 5 generations, the screening that backcrosses obtains Horticultural Characters, resisting verticillium close with " Suzhou ox horn " and fusarium wilt eggplant cytoplasm male sterility line.
Progeny population carries out the qualification of fertility, resistance to verticillium wilt, fusarium wilt disease resistance and Horticultural Characters, and concrete authentication method is as follows:
Fertility identification: select Anther degeneration type or pollen sterility type plant;
Resistance to verticillium wilt is identified: adopt fill with root inoculation method in Seedling Inoculation concentration be 1 × 10
7individual spore/ml verticillium wilt pathogen, verticillium wilt bacterial strain is VW003, inoculates after 30 days, investigation eggplant seedling incidence, select disease level be 0 or 1 disease-resistant individual plant, severity Scaling standard is: 5 grades, asymptomatic; 4 grades, slightly wilt, 1/5 has the slight macula lutea of small size with lower blade; 3 grades, wilt serious, 1/5 ~ 1/2 blade has obvious macula lutea, and 1 true leaf comes off; 2 grades, symptom is obvious, wilts serious, 1/2 ~ 2/3 yellowing leaf, and 2 true leaves come off; 1 grade, symptom is obvious, wilting especially severe, more than 2/3 yellowing leaf, and 3 ~ 4 true leaves come off; 0 grade, except lobus cardiacus, 2/3 comes off with blade wilting, close to dead;
Fusarium wilt disease resistance is identified: adopt fill with root inoculation method in Seedling Inoculation concentration be 1 × 10
7individual spore/ml Pathogen of Fusarium Wilt, fusarium wilt bacterial strain is FW007, in inoculation after 30 days, investigation eggplant seedling incidence, the selection state of an illness is the disease-resistant individual plant of 1 ~ 2 grade, and severity Scaling standard is: 1 grade, asymptomatic; 2 grades, 1 ~ 2 cotyledon comes off; 3 grades, yellowing leaf; 4 grades, symptom is obvious, wilts serious; 5 grades, close to dead;
The qualification of Horticultural Characters: select and " Suzhou ox horn " plant that Horticultural Characters is close.Being accredited as of concrete Horticultural Characters selects flower purple, fruit clavate, commodity fruit pericarp purple, vertical footpath 25-30cm, the plant of transverse diameter 5.0-6.0cm.
beneficial effect
Compared with the prior art, the present invention is by hybridizing, backcrossing and be polymerized male sterile, resistance to verticillium wilt, fusarium wilt disease resistance and excellent Horticultural Characters, and its beneficial effect is: sterile lineage non-pollen type male sterile line, sterile plant rate 100%; Male sterile line resisting verticillium and fusarium wilt; Male sterile line has excellent Horticultural Characters.
four, accompanying drawing explanation
Fig. 1 African red eggplant plant
Fig. 2
s. linnaeanumplant
Fig. 3 " Suzhou ox horn " plant
Fig. 4 SaetC1 is more left with " Suzhou ox horn " flower pesticide form is SaetC1, and flower pesticide is flat, without pollen; The right side is " Suzhou ox horn ", and flower pesticide is full, has pollen
Fig. 5 SaetC1 plant
five, embodiment
1, vegetable material and source
(African red eggplant × seven leaf eggplant) F
2colony: Interspecific Hybrids Fertility segregation colony.
African red eggplant
s. aethiopicumgroup
aculeatum(Centre for Genetic Resource, theNetherlands externally provides), for being distributed in the eggplant sibling species in Africa, is the donor of cytoplasmic male sterile gene and anti-blight gene in the present invention; Floral white, fruit is oblate, tender fruit pericarp light green, and aging fruit fruit peel orange is red, average vertical footpath 4 cm, transverse diameter 5cm.
" seven leaf eggplants " (China national Germplasm Resources of Vegetables storehouse in mid-term externally provides) is by Beijing landrace; Flower purple, fruit is oblate, commodity fruit pericarp purple, average vertical footpath 10 cm, transverse diameter 15cm.
(wildness eggplant (
s. linnaeanum) × Suzhou ox horn) BC
4(backcross 4 generation colony) colony: Interspecific Hybrids resistance to verticillium wilt segregation population.
Wildness eggplant
s. linnaeanum(United States Department of Agriculture-Agricultural Research Service externally provides), for being distributed in the eggplant sibling species in Africa, is the donor of resisting verticillium gene in the present invention; Flower purple, fruit subcircular, tender fruit pericarp green band hickie, aging fruit pericarp is faint yellow, average vertical footpath 3 cm, transverse diameter 3cm.
" Suzhou ox horn " (China national Germplasm Resources of Vegetables storehouse in mid-term externally provides) is Jiangsu Province's landrace; Flower purple, fruit clavate, commodity fruit pericarp purple, average vertical footpath 5 cm, transverse diameter 30cm.
2, the hybridization of objective trait is polymerized and qualification
From (African red eggplant × seven leaf eggplant) F
2mass screening
without pollen and anti-blight individual plant, with (
s. linnaeanum× Suzhou ox horn) BC
4in colony
resisting verticillium individual plant is male parenthybridize, to filial generation carry out evaluation and screening obtain resisting verticillium and fusarium wilt without the sterile strain of pollen types.
3, the seed selection of " SaetC1 "
To the above resisting verticillium that obtains and fusarium wilt without the sterile strain of pollen types, with " Suzhou ox horn " for male parent carries out backcrossing of continuous 4 generations.Each backcross generations colony is carried out to the qualification of fertility, resistance to verticillium wilt, fusarium wilt disease resistance and Horticultural Characters, concrete authentication method is as follows:
Fertility identification: carry out field observation to the flower structure of each backcross generations colony and flowering habit, selects Anther degeneration type or pollen sterility type plant.
Resistance to verticillium wilt is identified: with reference to Jing Li army etc., the Primary Study of eggplant resisting verticillium heredity, gardening journal, 2000,27(4): the method for 293-294, adopt fill with root inoculation method in Seedling Inoculation concentration be 1 × 10
7individual spore/ml verticillium wilt pathogen, verticillium wilt bacterial strain is that VW003(is intelligent etc. for dawn in week, causes the Isolation and ldentification of eggplant yellow wilting pathogen, Jiangsu's agriculture science, 2012,40(12): 127-129).Inoculate after 30 days, investigation eggplant seedling incidence, select disease level be 0 or 1 disease-resistant individual plant, severity Scaling standard is: 5 grades, asymptomatic; 4 grades, slightly wilt, 1/5 has the slight macula lutea of small size with lower blade; 3 grades, wilt serious, 1/5 ~ 1/2 blade has obvious macula lutea, and 1 true leaf comes off; 2 grades, symptom is obvious, wilts serious, 1/2 ~ 2/3 yellowing leaf, and 2 true leaves come off; 1 grade, symptom is obvious, wilting especially severe, more than 2/3 yellowing leaf, and 3 ~ 4 true leaves come off; 0 grade, except lobus cardiacus, 2/3 comes off with blade wilting, close to dead.
Fusarium wilt disease resistance is identified: with reference to Mutlu etc., Development of SRAP, SRAP-RGA, RAPD and SCAR markers linked with a Fusarium wilt resistance gene in eggplant, Theor Appl Genet, the method of 2008,117:1303-1312, adopt fill with root inoculation method in Seedling Inoculation concentration be 1 × 10
7individual spore/ml Pathogen of Fusarium Wilt, fusarium wilt bacterial strain is that FW007(is intelligent etc. for dawn in week, cause the Isolation and ldentification of eggplant yellow wilting pathogen, Jiangsu's agriculture science, 2012,40(12): 127-129) after 30 days, investigate eggplant seedling incidence in inoculation, with reference to Cappelli etc., Source of resistance among Solanum spp. to an Italian isolate of
fusariumoxysporumf. sp. melongenae, Eucarpia Meeting on genetics and breeding of Capsicum & Eggplant. IX, 1995, the sick grade standard of 221 ~ 224, the selection state of an illness is the disease-resistant individual plant of 1 ~ 2 grade, and severity Scaling standard is: 1 grade, asymptomatic; 2 grades, 1 ~ 2 cotyledon comes off; 3 grades, yellowing leaf; 4 grades, symptom is obvious, wilts serious; 5 grades, close to dead.
The qualification of Horticultural Characters: each backcross generations colony is selected and " Suzhou ox horn " strain that Horticultural Characters is close, flower purple, fruit clavate, commodity fruit pericarp purple, vertical footpath 25-30cm, transverse diameter 5.0-6.0cm.
Along with the increase of the algebraically that backcrosses, in colony, the ratio of sterile strain increases gradually, and main Horticultural Characters is close to " Suzhou ox horn ".Backcross after 5 generations, in colony, each individual plant all shows sterile, and Horticultural Characters is also close with " Suzhou ox horn ", fruit clavate, pericarp atropurpureus, uniform coloring, and glossiness is strong, and bearing fruit property is good.Thereby is achieved resisting verticillium and fusarium wilt eggplant kytoplasm fertility male sterile line, called after " SaetC1 ".
The feature of " SaetC1 ": non-pollen type male sterile line, sterile plant rate 100%; Begin flower joint position 10 ~ 11 joint; Fruit clavate, vertical footpath 25-30cm, transverse diameter 5.0-6.0cm, the black purple of commodity pericarp; Resisting verticillium and fusarium wilt.
Claims (5)
1. a selection for resisting verticillium and fusarium wilt eggplant cytoplasm male sterility line, comprising: with African red eggplant (
solanum aethiopicumgroup
aculeatum) with " seven leaf eggplants " Interspecific Hybrids in anti-blight male sterile plant be female parent, with wildness eggplant (
s. linnaeanum) be that male parent is hybridized with resisting verticillium individual plant in " Suzhou ox horn " Interspecific Hybrids, hybridization is carried out to the qualification of fertility, resistance to verticillium wilt and fusarium wilt disease resistance for colony, screening obtains the male sterile plant of anti-blight and verticillium wilt two kinds of diseases.
2. the selection of a kind of resisting verticillium according to claim 1 and fusarium wilt eggplant cytoplasm male sterility line, it is characterized in that, claim 1 is screened to the male sterile plant of anti-blight and the verticillium wilt two kinds of diseases obtained, with " Suzhou ox horn " for male parent backcrosses, each backcross generations colony is carried out to the qualification of fertility, resistance to verticillium wilt, fusarium wilt disease resistance and Horticultural Characters, through 5 generations, the screening that backcrosses obtains Horticultural Characters, resisting verticillium close with " Suzhou ox horn " and fusarium wilt eggplant cytoplasm male sterility line.
3. the selection of a kind of resisting verticillium according to claim 1 and 2 and fusarium wilt eggplant cytoplasm male sterility line, is characterized in that, the concrete grammar of progeny population Screening and Identification is as follows:
Fertility identification: select Anther degeneration type or pollen sterility type plant;
Resistance to verticillium wilt is identified: adopt fill with root inoculation method in Seedling Inoculation concentration be 1 × 10
7individual spore/ml verticillium wilt pathogen, verticillium wilt bacterial strain is VW003, inoculates after 30 days, investigation eggplant seedling incidence, select disease level be 0 or 1 disease-resistant individual plant, severity Scaling standard is: 5 grades, asymptomatic; 4 grades, slightly wilt, 1/5 has the slight macula lutea of small size with lower blade; 3 grades, wilt serious, 1/5 ~ 1/2 blade has obvious macula lutea, and 1 true leaf comes off; 2 grades, symptom is obvious, wilts serious, 1/2 ~ 2/3 yellowing leaf, and 2 true leaves come off; 1 grade, symptom is obvious, wilting especially severe, more than 2/3 yellowing leaf, and 3 ~ 4 true leaves come off; 0 grade, except lobus cardiacus, 2/3 comes off with blade wilting, close to dead;
Fusarium wilt disease resistance is identified: adopt fill with root inoculation method in Seedling Inoculation concentration be 1 × 10
7individual spore/ml Pathogen of Fusarium Wilt, fusarium wilt bacterial strain is FW007, in inoculation after 30 days, investigation eggplant seedling incidence, the selection state of an illness is the disease-resistant individual plant of 1 ~ 2 grade, and severity Scaling standard is: 1 grade, asymptomatic; 2 grades, 1 ~ 2 cotyledon comes off; 3 grades, yellowing leaf; 4 grades, symptom is obvious, wilts serious; 5 grades, close to dead;
The qualification of Horticultural Characters: select and " Suzhou ox horn " plant that Horticultural Characters is close.
4. the selection of a kind of resisting verticillium according to claim 2 and fusarium wilt eggplant cytoplasm male sterility line, is characterized in that, flower purple is selected in the qualification of Horticultural Characters, fruit clavate, commodity fruit pericarp purple, vertical footpath 25-30cm, the plant of transverse diameter 5.0-6.0cm.
5. the selection of a kind of resisting verticillium according to claim 3 and fusarium wilt eggplant cytoplasm male sterility line, is characterized in that, flower purple is selected in the qualification of Horticultural Characters, fruit clavate, commodity fruit pericarp purple, vertical footpath 25-30cm, the plant of transverse diameter 5.0-6.0cm.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110338055A (en) * | 2019-05-17 | 2019-10-18 | 扬州大学 | A kind of eggplant anti-blight rapid identification method |
| CN112931191A (en) * | 2021-04-22 | 2021-06-11 | 重庆市农业科学院 | Breeding method of bacterial wilt-resistant male sterile eggplant rootstock |
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2014
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Non-Patent Citations (4)
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| 庄勇等: "茄子近缘种引种及种间杂交研究", 《上海农业学报》, vol. 23, no. 3, 30 September 2007 (2007-09-30), pages 34 - 37 * |
| 庄勇等: "非洲红茄与栽培茄种间杂种及其回交后代的鉴定", 《江苏农业学报》, vol. 27, no. 1, 28 February 2011 (2011-02-28), pages 137 - 140 * |
| 柳李旺等: "茄子（S.melongena L.）与其野生种远缘杂交研究进展", 《园艺学进展》, no. 2, 31 December 1998 (1998-12-31), pages 417 - 424 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110338055A (en) * | 2019-05-17 | 2019-10-18 | 扬州大学 | A kind of eggplant anti-blight rapid identification method |
| CN112931191A (en) * | 2021-04-22 | 2021-06-11 | 重庆市农业科学院 | Breeding method of bacterial wilt-resistant male sterile eggplant rootstock |
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| CN104335894B (en) | 2016-03-02 |
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