CN104335894B - The selection of a kind of resisting verticillium and fusarium wilt eggplant cytoplasm male sterility line - Google Patents
The selection of a kind of resisting verticillium and fusarium wilt eggplant cytoplasm male sterility line Download PDFInfo
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- CN104335894B CN104335894B CN201410638018.4A CN201410638018A CN104335894B CN 104335894 B CN104335894 B CN 104335894B CN 201410638018 A CN201410638018 A CN 201410638018A CN 104335894 B CN104335894 B CN 104335894B
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Abstract
The present invention relates to the selection of a kind of resisting verticillium and fusarium wilt eggplant cytoplasm male sterility line, belong to field of plant variety breeding technology.With African red eggplant <i>S</iGreatT.Gr eaT.GT<i>olanum</i><i>? aethiopicum</i>? group<i>? Aculeatum</i> is maternal with the male sterile plant of anti-blight in " seven leaf eggplants " Interspecific Hybrids, with wildness eggplant <i>S</iGreatT.Gr eaT.GT<i>.</iGre atT.GreaT.GT<i>? linnaeanum</i> is that male parent is hybridized with resisting verticillium individual plant in " Suzhou ox horn " Interspecific Hybrids, the male sterile plant of resisting verticillium and fusarium wilt is selected from filial generation, with " Suzhou ox horn " for male parent continuous backcross 5 generation, pass through fertility, resistance to verticillium wilt, the observation qualification of fusarium wilt disease resistance and Horticultural Characters, select male sterile line " SaetC1 ".Male sterile line " SaetC1 " belongs to non-pollen type male sterile line, sterile plant rate 100%; Precocity, flower joint position 10 ~ 11 of beginning is saved; Fruit clavate, vertical footpath 25-30cm, transverse diameter 5.0-6.0cm, the black purple of commodity pericarp; Resisting verticillium and fusarium wilt, can be used as the female parent of seed selection eggplant Hybrid.
Description
One, technical field
The present invention relates to the selection of a kind of resisting verticillium and fusarium wilt eggplant cytoplasm male sterility line, belong to field of plant variety breeding technology, be exclusively used in the seed selection of resisting verticillium and fusarium wilt eggplant cytoplasm male sterility line.
Two, background technology
Eggplant (Solanummelongena) is a kind of important vegetable crop of China.Eggplant verticillium wilt and fusarium wilt are the 2 kinds of important soil-borne diseases caused by verticillium dahliae and Fusarium oxysporum respectively.In recent years, the occurrence and harm of these 2 kinds of diseases is further serious, and becomes the important disease of eggplant facility cultivation, very urgent to the demand of resisting verticillium and fusarium wilt Eggplant Varieties in production.
Cytoplasmic male sterilty (cytoplasmicmalesterility, CMS) extensively exists in higher plant, in plant hybrid use of advantage, have important effect.In the Hybrid seed produces of self pollination crop, utilize male sterile line not only can save artificial emasculation cost, improve the purity of seed, and for preventing parent to run off, protecting the intellectual property has important effect.
Research shows, distant hybridization is the effective means of initiative male sterile line.In earlier stage, we are finding male sterile plant to cultivating in eggplant to import in the Interspecific Hybrids colony of African red eggplant anti-blight gene, backcross with resisting verticillium breeding material and excellent purple long eggplant inbred line and sterile strain further, to selecting resisting verticillium and fusarium wilt eggplant cytoplasm male sterility line, for New Eggplant Varieties seed selection lays the foundation.
Three, summary of the invention
Technical problem
The object of the invention is to overcome during current eggplant hybrid seed is produced needs artificial emasculation and existing kind to verticillium wilt and the weak deficiency of fusarium wilt disease resistance, provides a kind of selection of eggplant cytoplasm male sterility line.The male sterile line of seed selection does not produce pollen by this method, and sterile plant rate is high, and has the good characteristic of resisting verticillium and fusarium wilt, can be used as the female parent of seed selection eggplant Hybrid.
Technical scheme
The male-sterile line breeding method that the present invention provides is the selection of male sterile, resistance to verticillium wilt, the hybridization polymerization of fusarium wilt disease resistance and excellent Horticultural Characters, backcross improvement and offspring thereof.
A selection for resisting verticillium and fusarium wilt eggplant cytoplasm male sterility line, comprising:
Be female parent with the anti-blight male sterile plant in African red eggplant (SolanumaethiopicumgroupAculeatum) and " seven leaf eggplants " Interspecific Hybrids, be that male parent is hybridized with resisting verticillium individual plant in wildness eggplant (S.linnaeanum) and " Suzhou ox horn " Interspecific Hybrids, filial generation colony is carried out to the qualification of fertility, resistance to verticillium wilt and fusarium wilt disease resistance, screening obtains the male sterile plant of anti-blight and verticillium wilt two kinds of diseases.
To screen the anti-blight and the male sterile plant of verticillium wilt two kinds of diseases that obtain, with " Suzhou ox horn " for male parent backcrosses, each backcross generations colony is carried out to the qualification of fertility, resistance to verticillium wilt, fusarium wilt disease resistance and Horticultural Characters, through 5 generations, the screening that backcrosses obtains Horticultural Characters, resisting verticillium close with " Suzhou ox horn " and fusarium wilt eggplant cytoplasm male sterility line.
Progeny population carries out the qualification of fertility, resistance to verticillium wilt, fusarium wilt disease resistance and Horticultural Characters, and concrete authentication method is as follows:
Fertility identification: select Anther degeneration type or pollen sterility type plant;
Resistance to verticillium wilt is identified: adopt fill with root inoculation method in Seedling Inoculation concentration be 1 × 10
7individual spore/ml verticillium wilt pathogen, verticillium wilt bacterial strain is VW003, inoculates after 30 days, investigation eggplant seedling incidence, select disease level be 4 or 5 disease-resistant individual plant, severity Scaling standard is: 5 grades, asymptomatic; 4 grades, slightly wilt, 1/5 has the slight macula lutea of small size with lower blade; 3 grades, wilt serious, 1/5 ~ 1/2 blade has obvious macula lutea, and 1 true leaf comes off; 2 grades, symptom is obvious, wilts serious, 1/2 ~ 2/3 yellowing leaf, and 2 true leaves come off; 1 grade, symptom is obvious, wilting especially severe, more than 2/3 yellowing leaf, and 3 ~ 4 true leaves come off; 0 grade, except lobus cardiacus, 2/3 comes off with blade wilting, close to dead;
Fusarium wilt disease resistance is identified: adopt fill with root inoculation method in Seedling Inoculation concentration be 1 × 10
7individual spore/ml Pathogen of Fusarium Wilt, fusarium wilt bacterial strain is FW007, in inoculation after 30 days, investigation eggplant seedling incidence, the selection state of an illness is the disease-resistant individual plant of 1 ~ 2 grade, and severity Scaling standard is: 1 grade, asymptomatic; 2 grades, 1 ~ 2 cotyledon comes off; 3 grades, yellowing leaf; 4 grades, symptom is obvious, wilts serious; 5 grades, close to dead;
The qualification of Horticultural Characters: select and " Suzhou ox horn " plant that Horticultural Characters is close.Being accredited as of concrete Horticultural Characters selects flower purple, fruit clavate, commodity fruit pericarp purple, vertical footpath 25-30cm, the plant of transverse diameter 5.0-6.0cm.
Beneficial effect
Compared with the prior art, the present invention is by hybridizing, backcrossing and be polymerized male sterile, resistance to verticillium wilt, fusarium wilt disease resistance and excellent Horticultural Characters, and its beneficial effect is: sterile lineage non-pollen type male sterile line, sterile plant rate 100%; Male sterile line resisting verticillium and fusarium wilt; Male sterile line has excellent Horticultural Characters.
Four, accompanying drawing explanation
Fig. 1 African red eggplant plant
Fig. 2 S.linnaeanum plant
Fig. 3 " Suzhou ox horn " plant
Fig. 4 SaetC1 is more left with " Suzhou ox horn " flower pesticide form is SaetC1, and flower pesticide is flat, without pollen; The right side is " Suzhou ox horn ", and flower pesticide is full, has pollen
Fig. 5 SaetC1 plant
Five, embodiment
1, vegetable material and source
(African red eggplant × seven leaf eggplant) F
2colony: Interspecific Hybrids Fertility segregation colony.
African red eggplant S.aethiopicumgroupAculeatum (CentreforGeneticResource, theNetherlands externally provides) for being distributed in the eggplant sibling species in Africa, be the donor of cytoplasmic male sterile gene and anti-blight gene in the present invention; Floral white, fruit is oblate, tender fruit pericarp light green, and aging fruit fruit peel orange is red, average vertical footpath 4cm, transverse diameter 5cm.
" seven leaf eggplants " (China national Germplasm Resources of Vegetables storehouse in mid-term externally provides) is by Beijing landrace; Flower purple, fruit is oblate, commodity fruit pericarp purple, average vertical footpath 10cm, transverse diameter 15cm.
(wildness eggplant (S.linnaeanum) × Suzhou ox horn) BC
4(backcross 4 generation colony) colony: Interspecific Hybrids resistance to verticillium wilt segregation population.
Wildness eggplant S.linnaeanum (UnitedStatesDepartmentofAgriculture-AgriculturalResearch Service externally provides), for being distributed in the eggplant sibling species in Africa, is the donor of resisting verticillium gene in the present invention; Flower purple, fruit subcircular, tender fruit pericarp green band hickie, aging fruit pericarp is faint yellow, average vertical footpath 3cm, transverse diameter 3cm.
" Suzhou ox horn " (China national Germplasm Resources of Vegetables storehouse in mid-term externally provides) is Jiangsu Province's landrace; Flower purple, fruit clavate, commodity fruit pericarp purple, average vertical footpath 5cm, transverse diameter 30cm.
2, the hybridization of objective trait is polymerized and qualification
From (African red eggplant × seven leaf eggplant) F
2mass screening
without pollen and anti-blight individual plant, with (S.linnaeanum × Suzhou ox horn) BC
4in colony
resisting verticillium individual plant is male parenthybridize, to filial generation carry out evaluation and screening obtain resisting verticillium and fusarium wilt without the sterile strain of pollen types.
3, the seed selection of " SaetC1 "
To the above resisting verticillium that obtains and fusarium wilt without the sterile strain of pollen types, with " Suzhou ox horn " for male parent carries out backcrossing of continuous 4 generations.Each backcross generations colony is carried out to the qualification of fertility, resistance to verticillium wilt, fusarium wilt disease resistance and Horticultural Characters, concrete authentication method is as follows:
Fertility identification: carry out field observation to the flower structure of each backcross generations colony and flowering habit, selects Anther degeneration type or pollen sterility type plant.
Resistance to verticillium wilt is identified: with reference to Jing Li army etc., the Primary Study of eggplant resisting verticillium heredity, gardening journal, the method for 2000,27 (4): 293-294, adopt fill with root inoculation method in Seedling Inoculation concentration be 1 × 10
7individual spore/ml verticillium wilt pathogen, verticillium wilt bacterial strain is VW003 (Zhou Xiaohui etc. cause the Isolation and ldentification of eggplant yellow wilting pathogen, Jiangsu's agriculture science, 2012,40 (12): 127-129).Inoculate after 30 days, investigation eggplant seedling incidence, select disease level be 4 or 5 disease-resistant individual plant, severity Scaling standard is: 5 grades, asymptomatic; 4 grades, slightly wilt, 1/5 has the slight macula lutea of small size with lower blade; 3 grades, wilt serious, 1/5 ~ 1/2 blade has obvious macula lutea, and 1 true leaf comes off; 2 grades, symptom is obvious, wilts serious, 1/2 ~ 2/3 yellowing leaf, and 2 true leaves come off; 1 grade, symptom is obvious, wilting especially severe, more than 2/3 yellowing leaf, and 3 ~ 4 true leaves come off; 0 grade, except lobus cardiacus, 2/3 comes off with blade wilting, close to dead.
Fusarium wilt disease resistance is identified: with reference to Mutlu etc., DevelopmentofSRAP, SRAP-RGA, RAPDandSCARmarkerslinkedwithaFusariumwiltresistancegenei neggplant, TheorApplGenet, the method of 2008,117:1303-1312, adopt fill with root inoculation method in Seedling Inoculation concentration be 1 × 10
7individual spore/ml Pathogen of Fusarium Wilt, fusarium wilt bacterial strain is FW007 (Zhou Xiaohui etc., cause the Isolation and ldentification of eggplant yellow wilting pathogen, Jiangsu's agriculture science, 2012, 40 (12): 127-129) in inoculation after 30 days, investigation eggplant seedling incidence, with reference to Cappelli etc., SourceofresistanceamongSolanumspp.toanItalianisolateofFu sariumoxysporumf.sp.melongenae, EucarpiaMeetingongeneticsandbreedingofCapsicum & Eggplant.IX, 1995, the sick grade standard of 221 ~ 224, the selection state of an illness is the disease-resistant individual plant of 1 ~ 2 grade, severity Scaling standard is: 1 grade, asymptomatic, 2 grades, 1 ~ 2 cotyledon comes off, 3 grades, yellowing leaf, 4 grades, symptom is obvious, wilts serious, 5 grades, close to dead.
The qualification of Horticultural Characters: each backcross generations colony is selected and " Suzhou ox horn " strain that Horticultural Characters is close, flower purple, fruit clavate, commodity fruit pericarp purple, vertical footpath 25-30cm, transverse diameter 5.0-6.0cm.
Along with the increase of the algebraically that backcrosses, in colony, the ratio of sterile strain increases gradually, and main Horticultural Characters is close to " Suzhou ox horn ".Backcross after 5 generations, in colony, each individual plant all shows sterile, and Horticultural Characters is also close with " Suzhou ox horn ", fruit clavate, pericarp atropurpureus, uniform coloring, and glossiness is strong, and bearing fruit property is good.Thereby is achieved resisting verticillium and fusarium wilt eggplant kytoplasm fertility male sterile line, called after " SaetC1 ".
The feature of " SaetC1 ": non-pollen type male sterile line, sterile plant rate 100%; Begin flower joint position 10 ~ 11 joint; Fruit clavate, vertical footpath 25-30cm, transverse diameter 5.0-6.0cm, the black purple of commodity pericarp; Resisting verticillium and fusarium wilt.
Claims (3)
1. the selection of a resisting verticillium and fusarium wilt eggplant cytoplasm male sterility line, comprise: with the anti-blight male sterile plant in African red eggplant (SolanumaethiopicumgroupAculeatum) and " seven leaf eggplants " Interspecific Hybrids for maternal, be that male parent is hybridized with resisting verticillium individual plant in wildness eggplant (S.linnaeanum) and " Suzhou ox horn " Interspecific Hybrids, fertility is carried out to filial generation colony, the qualification of resistance to verticillium wilt and fusarium wilt disease resistance, screening obtains the male sterile plant of anti-blight and verticillium wilt two kinds of diseases, it is characterized in that, the anti-blight that screening is obtained and the male sterile plant of verticillium wilt two kinds of diseases, with " Suzhou ox horn " for male parent backcrosses, fertility is carried out to each backcross generations colony, resistance to verticillium wilt, the qualification of fusarium wilt disease resistance and Horticultural Characters, through 5 generations backcross screening obtain Horticultural Characters close with " Suzhou ox horn ", resisting verticillium and fusarium wilt eggplant cytoplasm male sterility line.
2. the selection of a kind of resisting verticillium according to claim 1 and fusarium wilt eggplant cytoplasm male sterility line, is characterized in that, the concrete grammar of progeny population Screening and Identification is as follows:
Fertility identification: select Anther degeneration type or pollen sterility type plant;
Resistance to verticillium wilt is identified: adopt fill with root inoculation method in Seedling Inoculation concentration be 1 × 10
7individual spore/ml verticillium wilt pathogen, verticillium wilt bacterial strain is VW003, inoculates after 30 days, investigation eggplant seedling incidence, select disease level be 4 or 5 disease-resistant individual plant, severity Scaling standard is: 5 grades, asymptomatic; 4 grades, slightly wilt, 1/5 has the slight macula lutea of small size with lower blade; 3 grades, wilt serious, 1/5 ~ 1/2 blade has obvious macula lutea, and 1 true leaf comes off; 2 grades, symptom is obvious, wilts serious, 1/2 ~ 2/3 yellowing leaf, and 2 true leaves come off; 1 grade, symptom is obvious, wilting especially severe, more than 2/3 yellowing leaf, and 3 ~ 4 true leaves come off; 0 grade, except lobus cardiacus, 2/3 comes off with blade wilting, close to dead;
Fusarium wilt disease resistance is identified: adopt fill with root inoculation method in Seedling Inoculation concentration be 1 × 10
7individual spore/ml Pathogen of Fusarium Wilt, fusarium wilt bacterial strain is FW007, in inoculation after 30 days, investigation eggplant seedling incidence, the selection state of an illness is the disease-resistant individual plant of 1 ~ 2 grade, and severity Scaling standard is: 1 grade, asymptomatic; 2 grades, 1 ~ 2 cotyledon comes off; 3 grades, yellowing leaf; 4 grades, symptom is obvious, wilts serious; 5 grades, close to dead;
The qualification of Horticultural Characters: select and " Suzhou ox horn " plant that Horticultural Characters is close.
3. the selection of a kind of resisting verticillium according to claim 1 and 2 and fusarium wilt eggplant cytoplasm male sterility line, is characterized in that, flower purple is selected in the qualification of Horticultural Characters, fruit clavate, commodity fruit pericarp purple, vertical footpath 25-30cm, the plant of transverse diameter 5.0-6.0cm.
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Non-Patent Citations (4)
| Title |
|---|
| 茄子（S.melongena L.）与其野生种远缘杂交研究进展;柳李旺等;《园艺学进展》;19981231(第2期);417-424 * |
| 茄子抗病育种研究进展;魏小伞等;《中国蔬菜》;20100531(第10期);1-8 * |
| 茄子近缘种引种及种间杂交研究;庄勇等;《上海农业学报》;20070930;第23卷(第3期);34-37 * |
| 非洲红茄与栽培茄种间杂种及其回交后代的鉴定;庄勇等;《江苏农业学报》;20110228;第27卷(第1期);137-140 * |
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