CN105900831B - A kind of selection of the polygonal Wild cabbage type normal rapeseed kind of main inflorescence - Google Patents
A kind of selection of the polygonal Wild cabbage type normal rapeseed kind of main inflorescence Download PDFInfo
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- CN105900831B CN105900831B CN201610319833.3A CN201610319833A CN105900831B CN 105900831 B CN105900831 B CN 105900831B CN 201610319833 A CN201610319833 A CN 201610319833A CN 105900831 B CN105900831 B CN 105900831B
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- main inflorescence
- cabbage type
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- type rape
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The invention discloses a kind of selection of the polygonal Wild cabbage type normal rapeseed kind of main inflorescence.Comprise the following steps：A) using the polygonal cabbage type rape C GMCC NO.12262 of main inflorescence as male parent, using cabbage type rape strain as female parent, hybridized, obtain hybrid generation F1；B) with the hybrid generation F1For nonrecurrent parent, using the cabbage type rape strain as recurrent parent, it is returned, obtains backcross progeny.C) using the backcross progeny as nonrecurrent parent, using the cabbage type rape strain as recurrent parent, backcross progeny is obtained；D) backcross progeny is selfed, that is, obtains the polygonal conventional cabbage type rape variety of main inflorescence.Using this method can seed selection have " main inflorescence is polygonal " character normal rapeseed kind, as it is suitable gently simplification, high-density planting rape variety.
Description
Technical field
The invention belongs to rapeseed breeding field, it is related to a kind of seed selection side of the polygonal Wild cabbage type normal rapeseed kind of main inflorescence
Method.
Background technology
China is Rape-seed production big country, but in recent years, rapeseed cultivation area and total output but continuously significantly glide, mechanization
Low degree is its one of the main reasons.And the light simplified cultivation of rape and mechanized harvest are realized comprehensively, not only will be in cultivation skill
Improved to some extent in art, it is often more important that to have been dashed forward in the seed selection of suitable light simplified cultivation and mechanized harvest rape variety
It is broken.
Adapt to characteristic that the rape variety that high-density planting is suitable light simplified cultivation and mechanized harvest should possess it
One.There is research to think, for direct-sowing rape with the increase of planting density, the proportion that main inflorescence grain yield accounts for single plant yield can be by
32% progressively increases to about 60%, during high-density planting, knot mil(unit of angular measure), main inflorescence two economical characters of effective silique number of rape
Contribution to yield is maximum, so, compared to the rape of conventional density plantation, high-density planting rape answers emphasis to strengthen to tying angle
The seed selection of the effective silique number of density, main inflorescence.But for a long time, the reason such as natural selection and evolution makes rape form main flower
Sequence knot mil(unit of angular measure) is usually no more than 1.5/cm, and main inflorescence knot angle is usually no more than the species feature of 120, therefore, by normal
It is difficult the polygonal rape variety of seed selection main inflorescence to advise breeding material and method.
The content of the invention
It is an object of the invention to provide a kind of selection of the polygonal cabbage type rape variety of main inflorescence.
Method provided by the invention, it is that the polygonal character transformation of cabbage type rape C GMCC NO.12262 main inflorescence is arrived it
In his cabbage type rape strain, the polygonal Wild cabbage type normal rapeseed kind of seed selection main inflorescence.
Cabbage type rape main inflorescence is polygonal in the present invention refers to main inflorescence silique number more than 150.
The above method comprises the following steps：
A) using the cabbage type rape C GMCC NO.12262 as male parent, using the cabbage type rape strain as female parent, enter
Row hybridization, obtains hybrid generation F1；
B) with the hybrid generation F1For nonrecurrent parent, using the cabbage type rape strain as recurrent parent, the is carried out
Once it is returned, obtains backcross progeny；
C) using the backcross progeny as nonrecurrent parent, using the cabbage type rape strain as recurrent parent, carry out continuous
Backcrossing, obtains the polygonal cabbage type rape variety of main inflorescence.
In the above method,
The continuous backcross number is 2 times；
The nonrecurrent parent that the first time backcrossing is selected is the hybrid generation F1The most list of middle main inflorescence bud
Strain；
The nonrecurrent parent that each continuous backcross is selected is the individual plant that main inflorescence bud is most in last time backcross progeny.
In the above method,
In step C, after last time is returned, also comprise the following steps：
The last time is returned in obtained backcross progeny, the most individual plant of main inflorescence bud is chosen and carries out for the first time
Selfing, obtains first time self progeny；
The most individual plant of main inflorescence bud is chosen from first time self progeny again and carries out second of selfing, is obtained second
Self progeny.
In the above method, after described second is selfed, also comprise the following steps：Choose main in second of self progeny
The polygonal Wild cabbage type normal rapeseed kind of the most individual plant of inflorescence silique number, as main inflorescence.
In above-mentioned, the most individual plants of main inflorescence number of flowers are meet that main inflorescence number of flowers is the cabbage type rape strain 2
Times or more than 2 times individual plants.
Main inflorescence silique number individual plant is up to meet main inflorescence silique number for the cabbage type rape strain 2 times or 2 times
Above individual plant.
In the above method, the cabbage type rape strain is Wild cabbage type normal rapeseed strain ZH16, is that (state examines in double No. 3 of Zhejiang
2001003) (seed selection of crop institute of Shanghai academy of agricultural sciences, state examine 2006006) oil hybridizes after and formed through systematic breeding oil with Shanghai oil 17.
The stable new lines 12R1402 of " main inflorescence is polygonal " character is selected, by it on April 8th, 2016, in being deposited in
State's Microbiological Culture Collection administration committee common micro-organisms center (abbreviation CGMCC, address：BeiChen West Road, Chaoyang District, BeiJing City 1
Number institute 3, Institute of Microorganism, Academia Sinica, postcode 100101), preserving number is CGMCC NO.12262, plant classification
It is named as cabbage type rape.
The experiment proves that can be by " main inflorescence is polygonal " character by hybridizing, being returned and the transformation of selfing technology is to general
In logical cabbage type rape strain, the excellent normal rapeseed kind with " main inflorescence is polygonal " character of seed selection.As suitable light letter
Change, high-density planting rape variety.
Brief description of the drawings
Fig. 1 is the close oily No. 1 seed selection flow chart of " main inflorescence is polygonal " cabbage type rape variety.
Fig. 2 is that cabbage type rape ZH16 contrasts with the close oily No. 1 different times main inflorescence of " main inflorescence is polygonal " Brassica napus；
A, elongation cabbage type rape (left, ZH16) and " main inflorescence is polygonal " rape (right, close oily No. 1) main inflorescence flower bud pair
Than；
B, full-bloom stage cabbage type rape (left, ZH16) and " main inflorescence is polygonal " rape (right, close oily No. 1) main inflorescence are spent pair
Than；；
C, maturity period cabbage type rape (left, ZH16) and " main inflorescence is polygonal " rape (right, close oily No. 1) main inflorescence angle pair
Than.
Fig. 3 is cabbage type rape (left, ZH16) and " main inflorescence is polygonal " rape (right, close oily No. 1) main inflorescence individual plant seed
Yield comparison.
Embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
Material used, reagent etc., unless otherwise specified, are commercially obtained in following embodiments.
Main inflorescence is polygonal in the present embodiment refers to main inflorescence silique number more than 150.
Embodiment 1, seed selection " main inflorescence is polygonal " Wild cabbage type normal rapeseed kind
First, the discovery of " main inflorescence is polygonal " napus lines and seed selection
2004, the extremely more variation individual plant in one plant of main inflorescence knot angle is found in breeding material, selfing for years and
M8003 line, the stable new lines 12R1402 of " main inflorescence is polygonal " character, the main flower of the strain are selected in variant offspring
300, the effective silique number highest knot angle of sequence, average 180 or so.Select the stable new lines of " main inflorescence is polygonal " character
12R1402, by it on April 8th, 2016, it is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center
(abbreviation CGMCC, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode
100101), preserving number is CGMCC NO.12262, and the plant Classification And Nomenclature is cabbage type rape.
2nd, the seed selection of " main inflorescence is polygonal " Wild cabbage type normal rapeseed kind
In March, 2005 carries out hybridization transformation according to Fig. 1：
1) hybridize
In March, 2005, using 2 plants of Wild cabbage type normal rapeseed strain ZH16, (ZH16 was double No. 3 as Zhejiang using 5 plants of 12R1402 as male parent
(state examine oil 2001003) and Shanghai oil 17 (seed selection of crop institute of Shanghai academy of agricultural sciences, state examine 2006006) oil hybridizes after through systematic breeding and
Into) to be maternal, hybridization, obtain 10 parts of filial generation F1；
2) the 1st backcrossing
In May, 2005, take the hybrid seed of harvest to Qinghai and add generation.In July, 2005 is from 10 parts of F1Middle selection main inflorescence
The most individual plant of bud (main inflorescence number of flowers is about 2 times of ZH16), is denoted as F1, totally 10 plants；With 10 plants of F1As male parent (non-wheel
Return parent), 10 plants of ZH16 are recurrent parent, backcrossing, obtain 10 parts of 1 generation colony BC of backcrossing1F1；
3) the 2nd backcrossing
In March, 2006 is returned 1 generation colony BC from 10 parts1F1Most individual plant (the main inflorescence number of flowers of middle selection main inflorescence bud
About the 2 of ZH16 times), it is denoted as BC1F1- ZH16, totally 10 plants；
With this 10 plants of BC1F1- ZH16 is used as male parent, and 10 plants of ZH16 are recurrent parent, backcrossing, obtains 10 parts of backcrossing 2 generation colonies
BC2F1；
4) the 3rd backcrossing
In July, 2006 is returned 2 generation colony BC from 10 parts2F1Most individual plant (the main inflorescence number of flowers of middle selection main inflorescence bud
About the 2 of ZH16 times), it is denoted as BC2F1- ZH16, totally 10 plants；
With this 10 plants of BC2F1- ZH16 is used as male parent, and 10 plants of ZH16 are recurrent parent, backcrossing, obtains 10 parts of backcrossing 3 generation colonies
BC3F1；
5) the 1st selfing
In March, 2007 is returned 3 generation colony BC from 10 parts3F1Most individual plant (the main inflorescence number of flowers of middle selection main inflorescence bud
About the 2 of ZH16 times), it is denoted as BC3F1- ZH16, totally 10 plants；
To 10 plants of individual plants of selection, bagging selfing, 10 plants of 1 generation colony BC of selfing are obtained3F2；
6) the 2nd selfing
Plant 10 parts of 1 generation colony BC of selfing in July, 20073F2, every part is planted 100 plants, totally 10 strains, from averagely main flower
The most individual plant (main inflorescence number of flowers is about 2 times of ZH16) of main inflorescence bud is selected in the most strain of sequence silique number, is denoted as
BC3F3, totally 10 plants；
10 plants of individual plant baggings of mark are selfed, obtain 10 parts of 2 generation individual plant BC of selfing3F3；
7) isolation breeding
Plant 10 parts of 2 generation colony BC of selfing in March, 20083F3, every part is planted 100 plants, totally 10 strains, and each strain is first
Isolate breeding with the nylon wire account of 80 mesh respectively before spending, the maturity period investigates the master of the selfing 2 generation colony of 10 set account isolation breedings
Inflorescence silique number, screen during the most strain (main inflorescence silique number is about 2 times of ZH16) of average main inflorescence silique number is used as and select
" main inflorescence is polygonal " Wild cabbage type normal rapeseed kind, name as close oily No. 1.
By common cabbage type rape (left, ZH16) and " main inflorescence is polygonal " Brassica napus (right, close oily No. 1) different times master
Inflorescence contrasts, as a result such as Fig. 2, it can be seen that main inflorescence is polygonal " the average individual plant elongation main inflorescence flower of rape (right, close oily No. 1)
Flower bud number, full-bloom stage are bloomed number and maturity period knot angle number (average 175), to be significantly more than common cabbage type rape (left, ZH16)
Average individual plant elongation main inflorescence number of flowers, full-bloom stage are bloomed number and maturity period knot angle number (average 65).
By common cabbage type rape (left, ZH16) and " main inflorescence is polygonal " rape (right, close oily No. 1) average individual plant main inflorescence
Grain yield contrasts, as a result such as Fig. 3, it can be seen that " main inflorescence is polygonal " rape (right, close oily No. 1) average individual plant main inflorescence seed
Grain yield reaches 27.5g, is common cabbage type rape (left, ZH16) averagely more than 3 times of individual plant main inflorescence grain yield 8.5g.
Claims (3)
1. a kind of selection of the polygonal Wild cabbage type normal rapeseed kind of main inflorescence, is by cabbage type rape C GMCC NO.12262
The polygonal character transformation of main inflorescence into other cabbage type rape strains, the polygonal Wild cabbage type normal rapeseed kind of seed selection main inflorescence；
The main inflorescence is polygonal to refer to main inflorescence silique number more than 150；
Methods described comprises the following steps：
A）Using the cabbage type rape C GMCC NO.12262 as male parent, using the cabbage type rape strain as female parent, carry out miscellaneous
Hand over, obtain hybrid generation F1；
B）With the hybrid generation F1For nonrecurrent parent, using the cabbage type rape strain as recurrent parent, return for the first time
Hand over, obtain backcross progeny；
C）Using the backcross progeny as nonrecurrent parent, using the cabbage type rape strain as recurrent parent, continuous backcross is carried out,
Obtain the polygonal cabbage type rape variety of main inflorescence；
In step C, after last time is returned, also comprise the following steps：
The last time is returned in obtained backcross progeny, choose the most individual plant of main inflorescence bud carry out for the first time from
Hand over, obtain first time self progeny；
The most individual plant of main inflorescence bud is chosen from first time self progeny again and carries out second of selfing, obtains second of selfing
Offspring；
After described second is selfed, also comprise the following steps：It is most to choose main inflorescence silique number in second of self progeny
Individual plant, the as polygonal Wild cabbage type normal rapeseed kind of main inflorescence.
2. according to the method for claim 1, it is characterised in that：
The continuous backcross number is 2 times；
The nonrecurrent parent that the first time backcrossing is selected is the hybrid generation F1The most individual plant of middle main inflorescence bud；
The nonrecurrent parent that each continuous backcross is selected is the individual plant that main inflorescence bud is most in last time backcross progeny.
3. according to the method for claim 1, it is characterised in that：
The most individual plants of main inflorescence number of flowers are meet that main inflorescence number of flowers is the cabbage type rape strain 2 times or 2 times
Above individual plant；
The most individual plants of main inflorescence silique number are meet that main inflorescence silique number is the cabbage type rape strain 2 times or 2 times
Above individual plant.
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| CN110100722B (en) * | 2019-06-05 | 2022-03-01 | 安徽农业大学 | Breeding method of purple-leaf white-flower cabbage type landscape rape conventional variety |
| CN110073971B (en) * | 2019-06-05 | 2022-06-07 | 安徽农业大学 | Breeding method of purple-leaf white-flower cabbage type rape cytoplasmic male sterility restoring line |
| CN110741929A (en) * | 2019-11-21 | 2020-02-04 | 贵州省油菜研究所(贵州省油菜工程技术研究中心国家油菜改良中心贵州分中心) | Main inflorescence multi-pod cabbage type rape and breeding method thereof |
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| CN1539264A (en) * | 2003-09-27 | 2004-10-27 | 河南省农业科学院棉花油料作物研究所 | Method for preparing species of interbreed high oil containing rape |
| CN105475118B (en) * | 2015-12-01 | 2018-06-26 | 贵州省油菜研究所 | The selection of recessive short bar rape and application |
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