CN108377907A - A method of initiative sorghum male sterile line - Google Patents
A method of initiative sorghum male sterile line Download PDFInfo
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- CN108377907A CN108377907A CN201810338472.6A CN201810338472A CN108377907A CN 108377907 A CN108377907 A CN 108377907A CN 201810338472 A CN201810338472 A CN 201810338472A CN 108377907 A CN108377907 A CN 108377907A
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- generations
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The present invention provides a kind of method of initiative sorghum male sterile line：It is female parent with 314B, is male parent with 871300, by mostly generation selects lucky 2055B for many years；It is again female parent with lucky 2055B, obtains F1 generation by paternal hybrid of lucky 502B, F1 generation is selfed to obtain F2 generations, and F2 obtains F3 generations for individual plant selfing；In F3 generations, hybridize to obtain F1 generation with Ji Jinchang morning B as female parent, and F1 generation is selfed to obtain F2 generations, and F2 is continuously selfed for single plant, and screening obtains leaf of short stem narrow F4 generations；In F4 generations, continue roguing and are selfed to obtain F5 generations, while F4 generations obtain F1 generation with sterile line Ji 2055A test crosses；F5 is returned to obtain BC1 generations for roguing and F1 generation sterile plant, while F5 generation selfings obtain F6 generations；It by repeatedly continuous selfing and backcrossing, obtains backcrossing BC4 generations and is selfed F9 generations, male parent female parent is almost the same, carries out being measured cenospecies with restorer, therefrom selects plant type is good, special of short stem, coordinate force is high to be named as lucky 2065A, Ji 2065B.Spy sterile line Ji 2065A of short stem provided by the invention, the cenospecies plant that group is allotted is short, and regularity is high, is particularly suitable for mechanization production.
Description
Technical field
The invention belongs to crop breeding technical fields, and in particular to a method of initiative sorghum male sterile line.
Background technology
What is applied in China's sorghum production is cenospecies.What is applied in being produced before last century the eighties is high stalk hybridization
Sorghum is cooked braiding frame material etc. while producing grain using stalk；With the development and social progress of chemical industry, stalk
Purposes it is seldom, so production switchs to using suitable for the middle stalk the hybrid strain with high yield that manually harvests；With the rise of artificial price,
Production gradually switchs to mechanization, and there is an urgent need for cultivate the cenospecies of short stem of suitable for mechanization.And the height of cenospecies depends on parent's
Highly, to cultivate cenospecies of short stem just must first cultivate parental department of short stem.And be difficult by existing selection will be complicated
Quantitative character polymerize, and is not easy to select good plant type, wide adaptability, special parental department of short stem.
Invention content
It is difficult to select good plant type, the above problem of wide adaptability, special parental department of short stem for existing selection,
The present invention provides a kind of method of initiative sorghum male sterile line Ji 2065A, is repeatedly passed using multiple excellent materials of short stem
Grade hybridization, makes beneficial gene gradually polymerize, and the derived Dwarf Plants selfing of the good wide adaptability of plant type is selected from offspring, then transformation is not at
It educates and is.
The present invention is achieved by the following technical solutions：
A method of initiative sorghum male sterile line includes the following steps：
(1) it is female parent with 314B, is male parent with 871300, by mostly generation selects lucky 2055B for many years；
(2) it is female parent with lucky 2055B, obtains F1 generation by paternal hybrid of lucky 502B, F1 generation is selfed to obtain F2 generations, separation choosing
It selects excellent F2 of short stem and obtains F3 generations for individual plant selfing；
(3) in F3 generations, hybridize to obtain F1 generation with Ji Jinchang morning B as female parent, and F1 generation is selfed to obtain F2 generations, selects leaf of short stem narrow
F2 be continuously selfed for single plant, screening obtain leaf of short stem narrow F4 generations；
(4) individual plant selfing that F4 generations continue to select leaf of short stem narrow obtains F5 generations, while F4 generations and sterile line Ji 2055A test crosses
Obtain F1 generation；
(5) in F5 generations, continue the single plant for selecting leaf of short stem narrow and F1 generation sterile plant is returned to obtain BC1 generations, while F5 generations are certainly
Friendship obtains F6 generations；
(6) by repeatedly continuous selfing and backcrossing, backcrossing BC4 generations being obtained and are selfed F9 generations, male parent female parent is almost the same, with
Restorer carries out being measured cenospecies, therefrom selects plant type is good, special of short stem, coordinate force is high to be named as lucky 2065A, Ji 2065B.
Advantageous effect：
(1) present invention mainly applies graded selection cross maintainer, so that beneficial gene is gradually polymerize, is selected from offspring
The derived Dwarf Plants of the good wide adaptability of plant type are selfed, then transformation at sterile line, i.e. parent 1 hybridizes with parent 2, offspring's roguing again with
Parent 3 hybridizes, and roguing hybridizes with parent 4 again, by this good strain of short stem of graded selection cross plant type again transformation at new infertility
System.
(2) formulated out that plant type is good, special sorghum male sterile line Ji 2065A of short stem using this method, the sterile line plant height
70-80 centimetres, blade is narrow, and plant type is good, 116 days or so breeding time；Seedling green, 25 centimetres or so of spike length, the red pale red grain of shell are matched
Resultant force is high.
(3) the spy sterile line Ji 2065A of short stem of application initiative, group are allotted suitable for mechanization elite hybrid of short stem, are prepared
Cenospecies height at 120-140 centimetres, it is of short stem, regularity is high, resistance to dense planting, suitable for mechanization production, welcome by peasant.
Description of the drawings
Fig. 1 is the initiative procedure chart of sorghum male sterile line Ji 2065A.
Specific implementation mode
The present invention is described in further details with reference to specific embodiment and attached drawing.
Embodiment 1 formulates sorghum male sterile line Ji 2065A
As shown in Figure 1, including the following steps：
(1) it is female parent with 314B, is male parent with 871300, by mostly generation selects lucky 2055B for many years；
(2) it is female parent with lucky 2055B, obtains F1 generation by paternal hybrid of lucky 502B, F1 generation is selfed to obtain F2 generations, separation choosing
It selects excellent F2 of short stem and obtains F3 generations for individual plant selfing；
(3) in F3 generations, hybridize to obtain F1 generation as maternal with Shanxi length morning B, and F1 generation is selfed to obtain F2 generations, selects leaf of short stem narrow
F2 is continuously selfed for single plant, and screening obtains leaf of short stem narrow F4 generations；
(4) individual plant selfing that F4 generations continue to select leaf of short stem narrow obtains F5 generations, while F4 generations and sterile line Ji 2055A test crosses
Obtain F1 generation；
(5) F5 is returned to obtain BC1 generations for roguing and F1 generation sterile plant, while F5 generation selfings obtain F6 generations；F6 generation after
Continuous roguing is returned to obtain BC2 generations with BC1, while selfing obtains F7 generations；In F7 generations, continue to be returned to obtain BC3 generations with BC2,
F7 generation selfings simultaneously obtain F8 generations；In F8 generations, continue roguing and are returned to obtain BC4 generations with BC3, while F8 generations are selfed to obtain
F9 generations.
In this way by multiple continuous selfing and multiple backcrossing, male parent female parent is almost the same, is measured with restorer
Cenospecies therefrom selects plant type is good, special of short stem, coordinate force is high to be named as lucky 2065A, Ji 2065B.
Parental source explanation：Lucky 2055B is from 314B and 871300B filial generations；Lucky 502B is black 30B and iron 239B
Filial generation.Ji Jinchang morning B are the maintainers of sorghum hybrid Ji miscellaneous 123, are introduced from sorghum research institute of academy of agricultural sciences of Shanxi Province
Progeny material, in Jilin locality, plantation breeding time is long, not good utilisation, precocious offspring is therefrom selected later, through more for many years
The selection and breeding in generation form, and since tassel is long, Ji Jinchang morning B are named as so rising.
The main feature and characteristic of sorghum male sterile line Ji 2065A
One, botanical characteristics：
70-80 centimetres of the sterile line plant height, blade is narrow, and plant type is good, 116 days or so breeding time；Seedling green, 25 lis of spike length
Rice or so, the red pale red grain of shell.
Two, biological characteristics：
(1) male sterility is stablized.
(2) florescence is long, column cap vitality is strong.
(3) coordinate force is high.
(4) lodging resistance is strong.
(5) numerous hybrid seed yield is high
Three, yield shows：
General clear kind of hectare yield is 3500-4500 kilograms.
Embodiment described above is only that the preferred embodiment of the present invention is described, not to the scope of the present invention
It is defined, under the premise of not departing from design spirit of the present invention, those of ordinary skill in the art are to technical scheme of the present invention
The various modifications made and improvement should all be fallen into the protection domain of claims of the present invention determination.
Claims (1)
1. a kind of method of initiative sorghum male sterile line, which is characterized in that include the following steps：
(1) it is female parent with 314B, is male parent with 871300, by mostly generation selects lucky 2055B for many years；
(2) it is female parent with lucky 2055B, obtains F1 generation by paternal hybrid of lucky 502B, F1 generation is selfed to obtain F2 generations, and separation selection is short
The excellent F2 of stalk obtains F3 generations for individual plant selfing；
(3) in F3 generations, hybridize to obtain F1 generation with Ji Jinchang morning B as female parent, and F1 generation is selfed to obtain F2 generations, the F2 for selecting leaf of short stem narrow
It is continuously selfed for single plant, screening obtains leaf of short stem narrow F4 generations；
(4) individual plant selfing that F4 generations continue to select leaf of short stem narrow obtains F5 generations, while F4 generations obtain with sterile line Ji 2055A test crosses
F1 generation；
(5) in F5 generations, continue the single plant for selecting leaf of short stem narrow and F1 generation sterile plant is returned to obtain BC1 generations, while F5 generations are selfed
To F6 generations；
(6) by repeatedly continuous selfing and backcrossing, backcrossing BC4 generations is obtained and are selfed F9 generations, male parent female parent is almost the same, with recovery
System carries out being measured cenospecies, therefrom selects plant type is good, special of short stem, coordinate force is high to be named as lucky 2065A, Ji 2065B.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810338472.6A CN108377907A (en) | 2018-04-16 | 2018-04-16 | A method of initiative sorghum male sterile line |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810338472.6A CN108377907A (en) | 2018-04-16 | 2018-04-16 | A method of initiative sorghum male sterile line |
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| Publication Number | Publication Date |
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| CN108377907A true CN108377907A (en) | 2018-08-10 |
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| CN201810338472.6A Pending CN108377907A (en) | 2018-04-16 | 2018-04-16 | A method of initiative sorghum male sterile line |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111512960A (en) * | 2020-05-25 | 2020-08-11 | 吉林省农业科学院 | Method for creating sorghum male sterile line Ji 701A by utilizing hierarchical hybrid polymerization early-maturing dwarf gene |
| CN112753571A (en) * | 2021-03-15 | 2021-05-07 | 四川省农业科学院水稻高粱研究所 | Breeding method of sorghum cytoplasmic male sterile line with high tannin content |
| CN114303935A (en) * | 2021-12-28 | 2022-04-12 | 吉林省农业科学院 | Method for creating glutinous sorghum sterile line Ji5535A with high combining ability by hybridizing backbone japonica sorghum parent lines |
| CN115380816A (en) * | 2022-08-25 | 2022-11-25 | 宜宾市农业科学院 | Method for creating sorghum maintainer line |
-
2018
- 2018-04-16 CN CN201810338472.6A patent/CN108377907A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111512960A (en) * | 2020-05-25 | 2020-08-11 | 吉林省农业科学院 | Method for creating sorghum male sterile line Ji 701A by utilizing hierarchical hybrid polymerization early-maturing dwarf gene |
| CN112753571A (en) * | 2021-03-15 | 2021-05-07 | 四川省农业科学院水稻高粱研究所 | Breeding method of sorghum cytoplasmic male sterile line with high tannin content |
| CN114303935A (en) * | 2021-12-28 | 2022-04-12 | 吉林省农业科学院 | Method for creating glutinous sorghum sterile line Ji5535A with high combining ability by hybridizing backbone japonica sorghum parent lines |
| CN115380816A (en) * | 2022-08-25 | 2022-11-25 | 宜宾市农业科学院 | Method for creating sorghum maintainer line |
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Application publication date: 20180810 |