CN103120118A - Method for breeding cabbage type rape recessive epistasis interaction genic male sterility two type line - Google Patents
Method for breeding cabbage type rape recessive epistasis interaction genic male sterility two type line Download PDFInfo
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Abstract
The invention discloses a method for a cabbage type rape recessive epistasis interaction genic male sterility two type line. The method selects a good recessive genic male sterility line B plant to be bagged and selfed so as to obtain a selfed sterile separated rape plant; the obtained selfed sterile separated rape plant is used as a recurrent parent to be back-crossed with the recessive epistasis interaction genic male sterility two type line; backcross descendant groups are selfed, and test cross and breeding are carried out to obtain a new recessive epistasis interaction genic male sterility homozygous two type line; and the recessive epistasis interaction genic male sterility homozygous two type line is subjected to test cross of a temporary maintainer line to obtain a full sterile line with 100% sterile plants. The method disclosed by the invention is simple in breeding procedure, high in speed and strong in operability; and the obtained homozygous two type line has genetic stability and high combining ability and can be conveniently applied to the breeding of new species of recessive epistasis interaction genic male sterility hybridized rape.
Description
Technical field
The invention belongs to the rapeseed breeding technical field, be specifically related to a kind of method of new recessive epistatic interaction in brassica napus genic male sterile two-type line seed selection.
Background technology
Rape has stronger hybrid vigour, and good hybrid combination generally can increase production 20%～30%.Since the Hybrided Rape Qingyou 2 of first authorization in 1985 and extensively plantation was bred, cross-bred rape production was very rapid in China's development.It is mainly rapeseed male sterility that rape hybridization at present utilizes approach, the key of rapeseed male sterility utilization is to obtain 100% sterile population when crossbreed produces, recessive karyon sterile two ties up to can only obtain 50% sterile population when crossbreed produces, crossbreed is taken a lot of work during manufacturing cost, and the purity of seed is difficult to guarantee, hybrid seed yield is also very low.Sterile being expected of nucleo-cytoplasmic interaction obtains 100% sterile population, but fertility instability easily is subject to the impact of environmental factor, has larger risk in hybrid seed production.The discovery of recessive cytoblast sterile epistatic gene not only makes the cultivation of 100% sterile population become possibility, simultaneously stable fertility, be not subjected to the impact of environmental factor.Yet although this sterile system has obvious advantage, the difficulty of seed selection is very large, has limited its extensive use in production practices.
Recessive cytoblast sterile two ties up to numerous rapeseed breeding units all long research history, has accumulated large quantities of floorboard with high oil content, high protein, basic material that the comprehensive agronomy proterties is good.Vast rapeseed breeding scientific worker is material based on this, the recessive nueleus sterility triseries material is carried out genetic improvement, but the cycle of each rapeseed breeding unit's seed selection is long, rare large, since Chen Fengxiang researcher found genic male sterile material 90l2AB in 1991 and proposes three interaction of genes patterns of this male sterile line fertility control, go through the breeding practice of 30 years, the core plasmic sterile three lines kind that whole nation authorization is promoted has just tens altogether.Therefore, further investigate the genetic development of this sterile system, improve technological means, seed selection cabbage type rape recessive cytoblast sterile material is that we face and not only have a problem in science of theory value but also tool application prospect.
Summary of the invention
The object of the invention is to provide the method for a kind of recessive epistatic interaction in brassica napus genic male sterile two-type line seed selection, reduce the difficulty of recessive epistatic interaction in brassica napus genic male sterile two-type line seed selection, accelerate the process of recessive epistatic interaction in brassica napus genic male sterile two-type line seed selection, this good pollination control system is applied to produce as early as possible easily, simultaneously, the homozygous two-type line coordinate force that obtains is strong, inheritance stability, and the program of seed selection is simple, workable, to overcome the deficiencies in the prior art.
For realizing purpose of the present invention, the present invention has adopted such technical scheme:
The method of recessive epistatic interaction in brassica napus genic male sterile two-type line of the present invention seed selection be take without Fertility segregation gms two-line system material as recurrent parent, at the new recessive epistatic interaction genic male sterile two-type line of backcross progeny seed selection, mainly comprise the following steps:
(1) select the selfing of good recessive cytoblast sterile two-line sterile line B strain bagging, obtain selfing without the Fertility segregation rapeseed plants; Good recessive cytoblast sterile two-line sterile line B strain refers to the two low and good recessive cytoblast sterile two-line sterile line B strains of comprehensive agronomy proterties of floorboard with high oil content, high protein, quality, its genotype be (
BnMs 1 ms 1 ms 2 ms 2 Or
Bnms 1 ms 1 Ms 2 ms 2 ).
(2) selfing that obtains in (1) without the Fertility segregation rapeseed plants as recurrent parent, backcross with the recessive epistatic interaction genic male sterile two-type line, hybridize in backcross progeny colony, selfing, test cross seed selection obtain the new sterile homozygous two-type line of recessive epistatic interaction core, its genotype be (
BnRfRfms 3 ms 3 ms 4 ms 4 *
BnRfRf Ms 3 ms 3 ms 4 ms 4 ).
(3) with the sterile homozygous two-type line of recessive epistatic interaction core and the temporary maintainer line test cross that obtain in (2), obtain the complete sterile line of sterile strain 100%, thereby be used for the seed selection of recessive nueleus sterility triseries new rape variety.
Further way is, described selfing is planted into the residential quarter without the Fertility segregation rapeseed plants, therefrom the menu strain is as female parent, and the sterile homozygous two of recessive epistatic interaction core to be improved is that male parent is hybridized, and filial generation and described backcrossing without Fertility segregation gms two-line system rape obtain BC
1F
1, BC
1F
1Selfing obtains BC
1F
2If self progeny's Fertility segregation ratio is 3:1 carries out paired test cross in this residential quarter, if Fertility segregation is than for 13:3,15:1 or 61:3 to the selfing of fertile plant bagging, compare for carrying out paired test cross in the residential quarter of 3:1 at self progeny's Fertility segregation, in the above in two kinds of situations test cross offspring Fertility segregation than for carrying out brother and sister's friendship in the residential quarter of 1:1, brother and sister hand over another branch of sterile individual plant and temporary maintainer line test cross, if brother and sister hand over Fertility segregation than being 1:1, with temporary maintainer line test cross complete sterility, be the sterile homozygous two-type line of recessive epistatic interaction core of having improved.
Compared with the prior art, the present invention has the following advantages:
(1) traditional breeding method is parent sire of hybrid pigs with conventional variety or product, the male sterile line later stage that seed selection obtains need to be made up in a large number and is measured, the workload that obtains cross combination which has strong superior is very large, and the present invention selects recessive cytoblast sterile two-line sterile line B strain offspring to be recurrent parent, to utilize male sterile line improvement male sterile line, be conducive to the division of male sterile line and restorer population, thereby easily be measured cross combination which has strong superior.
(2) traditional breeding method is directly take recessive cytoblast sterile two-line sterile line B strain as parent sire of hybrid pigs, filial generation is subject to gms two-line system and the two cover infertility-related gene regulation and control of core plasmic sterile three lines simultaneously, fertility is easily separated, be difficult to select the sterile homozygous two-type line of recessive epistatic interaction core of inheritance stability, and the present invention is to the selfing of recessive cytoblast sterile two-line sterile line B strain bagging, choosing is hybrid strain without the offspring of Fertility segregation, at the low sterile homozygous two-type line of core that can obtain inheritance stability from generation to generation.
(3) the present invention in hybridization for the first time namely take recessive cytoblast sterile two-line sterile line B strain offspring as maternal, thereby be conducive to be subjected to the merit of plasmon regulation and control to be delivered to filial generation.
(4) the method is workable, program seed selection is simple, and any scientific research breeding unit all can adopt, and has improved the Breeding Efficiency of Recessive epistatic GMS homozygous two-type line.
Embodiment
Below in conjunction with example, the present invention is described in further detail.
Embodiment 1:
(1) floorboard with high oil content, high protein, the two good recessive cytoblast sterile two-line sterile line B strain 09105B(of low comprehensive agronomy proterties of quality
BnMs 1 ms 1 ms 2 ms 2 Or
Bnms 1 ms 1 Ms 2 ms 2 ) select 3 strain bagging selfings, plant into 3 residential quarters, about each residential quarter plantation 200 strain, first residential quarter fertile plant wherein: sterile strain=152:60, second residential quarter fertile plant: sterile strain=167:65, the 3rd residential quarter fertile plant: sterile strain=158:44, wherein second residential quarter performance is relatively good, therefrom selects 10 strain fertile plant bagging selfings.
(2) 10 single-strain seeds of step (1) gained are planted into 10 different residential quarters, about each residential quarter plantation 100 strain, wherein there are 4 residential quarters there is no Fertility segregation, 6 residential quarter Fertility segregation ratios are near 3:1, from without 1 good residential quarter of choosing performance 4 residential quarters of Fertility segregation, gather in the crops 5 selfing single-strain seed 09105B(from this residential quarter
BnMs 1 Ms 1 ms 2 ms 2 Or
Bnms 1 ms 1 Ms 2 Ms 2 ).
(3) select the sterile homozygous two-type line 08121AB(of recessive epistatic interaction core to be improved
BnRfRfms 3 ms 3 ms 4 ms 4 *
BnRfRf Ms 3 ms 3 ms 4 ms 4 ) 3 brother and sister hand over the offspring to plant into 3 residential quarters, each residential quarter plantation 100 strains left and right are selected 1 to show residential quarter preferably from 3 residential quarters, and are selected 5 individual plants as male parent from this residential quarter, and step (2) is selected 5 fine individual plant 09105B(without the Fertility segregation residential quarter
BnMs 1 Ms 1 ms 2 ms 2 Or
Bnms 1 ms 1 Ms 2 Ms 2 ) hybridize for female parent and obtain 5 F1 for the hybridization individual plant.
(4) 5 F1 obtaining of step (3) hybridization become 5 residential quarters for the hybridization single-strain planting, and 5 individual plants that performance is good are selected from 1 residential quarter wherein in each residential quarter plantation 200 strains left and right, with step (2) without Fertility segregation gms two-line system B strain 09105B(
BnMs 1 Ms 1 ms 2 ms 2 Or
Bnms 1 ms 1 Ms 2 Ms 2 ) 5 individual plants of self progeny are that recurrent parent backcrosses with above-mentioned 5 selected individual plants respectively and obtains 5 BC
1F
1
(5) the backcross progeny BC of step (4)
1F
1Seed becomes 5 residential quarters, and each residential quarter plantation 200 strains left and right select 50 good individual plant bagging selfings of performance to obtain BC from 1 residential quarter wherein
1F
2
(6) BC of step (5)
1F
2Plant into 50 different residential quarters, about each residential quarter plantation 200 strain, found that 4 cell separation go out sterile strain, wherein first cell separation goes out 10 sterile strains, second cell separation goes out 7 sterile strains, the 3rd cell separation goes out 8 sterile strains, and the 4th cell separation goes out 7 sterile strains, from 50 individual plants of a residential quarter bagging selfing of Fertility segregation are arranged.
(7) step (6) self progeny plants into 50 different residential quarters, and each residential quarter plantation 200 strains left and right found that 4 residential quarter Fertility segregations than being 3:1, carries out therein 10 pairs of fertile plant and the paired test crosses of sterile strain in residential quarter.
(8) step (7) test cross offspring plants into 10 residential quarters, about each residential quarter plantation 200 strain, found that 3 residential quarters are without Fertility segregation, 7 residential quarter fertile plants and sterile strain Fertility segregation are than near 1:1, select one of them that Fertility segregation is arranged and show good residential quarter, carry out brother and sister in the residential quarter and hand over 5 pairs, another branch of sterile strain and the temporary maintainer line test cross of brother and sister's friendship simultaneously.
(9) step (8) offspring all plants into different districts, and each residential quarter plantation 200 strains left and right found that 5 couples of brother and sister hand over the whole Fertility segregations of offspring than near 1:1, with the test cross offspring sterile plant rate of temporary maintainer line be 100%.
Embodiment 2
(1) ~ (6) basic step is identical with embodiment 1, but material is 08112B, is the two low recessive cytoblast sterile two-line sterile line B strains of yellow seed.
(7) step (6) self progeny plants into 50 different residential quarters, about each residential quarter plantation 200 strain, found that 7 residential quarter Fertility segregations than near 13:3, select 1 Quality Residential District, from wherein selecting 50 strain fertile plant bagging selfings.
(8) step (7) self progeny plants into 50 different residential quarters, found that 8 residential quarter Fertility segregations than near 3:1, select wherein 1 Quality Residential District, and test cross is 10 pairs in pairs.
(9) the test cross offspring of step (8) plants into 10 residential quarters, about each residential quarter plantation 200 strain, found that 7 residential quarter test cross offspring Fertility segregations are than near 1:1,3 residential quarters are arranged without Fertility segregation, hand over 5 pairs at Fertility segregation than for carrying out brother and sister near the residential quarter of 1:1, brother and sister hand over another branch of sterile strain and temporary maintainer line test cross simultaneously.
(10) step (9) offspring all plants into different districts, and each residential quarter plantation 200 strains left and right found that 5 couples of brother and sister hand over the whole Fertility segregations of offspring than near 1:1, with the test cross offspring sterile plant rate of temporary maintainer line be 100%.
Certainly, the concrete exemplary applications of more than just inventing, the present invention also has other embodiment, and all employings are equal to the technical scheme of replacement or equivalent transformation formation, within all dropping on protection domain of the presently claimed invention.
Claims (2)
1. the method for recessive epistatic interaction in brassica napus genic male sterile two-type line seed selection, it is characterized in that take without Fertility segregation gms two-line system material as recurrent parent, at the new recessive epistatic interaction genic male sterile two-type line of backcross progeny seed selection, the method comprises the following steps:
(1) select the selfing of good recessive cytoblast sterile two-line sterile line B strain bagging, obtain selfing without the Fertility segregation rapeseed plants;
(2) selfing that obtains in (1) without the Fertility segregation rapeseed plants as recurrent parent, backcross with the recessive epistatic interaction genic male sterile two-type line, hybridize in backcross progeny colony, selfing, test cross seed selection obtain the new sterile homozygous two-type line of recessive epistatic interaction core;
(3) with the sterile homozygous two-type line of recessive epistatic interaction core and the temporary maintainer line test cross that obtain in (2), obtain the complete sterile line of sterile strain 100%.
2. the method for recessive epistatic interaction in brassica napus genic male sterile two-type line according to claim 1 seed selection, it is characterized in that: described selfing is planted into the residential quarter without the Fertility segregation rapeseed plants, therefrom the menu strain is as female parent, the sterile homozygous two of recessive epistatic interaction core to be improved is that male parent is hybridized, and filial generation and described backcrossing without Fertility segregation gms two-line system rape obtain BC
1F
1, BC
1F
1Selfing obtains BC
1F
2If self progeny's Fertility segregation ratio is 3:1 carries out paired test cross in this residential quarter, if Fertility segregation is than for 13:3,15:1 or 61:3 to the selfing of fertile plant bagging, compare for carrying out paired test cross in the residential quarter of 3:1 at self progeny's Fertility segregation, in the above in two kinds of situations test cross offspring Fertility segregation than for carrying out brother and sister's friendship in the residential quarter of 1:1, brother and sister hand over another branch of sterile individual plant and temporary maintainer line test cross, if brother and sister hand over Fertility segregation than being 1:1, with temporary maintainer line test cross complete sterility, be the sterile homozygous two-type line of recessive epistatic interaction core of having improved.
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| CN105830914A (en) * | 2016-04-14 | 2016-08-10 | 贵州粱丰农业科技有限公司 | Breeding method of cabbage-type rape high oil-content recessive epistatic interaction nuclear sterile homozygous two-type line |
| CN105850715A (en) * | 2016-04-14 | 2016-08-17 | 贵州粱丰农业科技有限公司 | Breeding method for cabbage-type rape high-oil-content recessive-epistatic-interaction nucleic-male-sterility homonuclear temporary maintaining line |
| CN108834879A (en) * | 2018-07-17 | 2018-11-20 | 安徽新安种业有限公司 | A kind of selection of homozygous double low recessive gms line rapes |
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| CN105850715A (en) * | 2016-04-14 | 2016-08-17 | 贵州粱丰农业科技有限公司 | Breeding method for cabbage-type rape high-oil-content recessive-epistatic-interaction nucleic-male-sterility homonuclear temporary maintaining line |
| CN108834879A (en) * | 2018-07-17 | 2018-11-20 | 安徽新安种业有限公司 | A kind of selection of homozygous double low recessive gms line rapes |
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