CN108834879A - A kind of selection of homozygous double low recessive gms line rapes - Google Patents
A kind of selection of homozygous double low recessive gms line rapes Download PDFInfo
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- CN108834879A CN108834879A CN201810784751.5A CN201810784751A CN108834879A CN 108834879 A CN108834879 A CN 108834879A CN 201810784751 A CN201810784751 A CN 201810784751A CN 108834879 A CN108834879 A CN 108834879A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides a kind of selection of homozygous double low recessive gms line rapes, belong to rape breeding technique field, hybridized with cabbage type rape Huyouza 1 offspring sterile plant with Shanghai oil 15, obtains homozygous double low recessive gms line rapes then in turn through hybridization, selfing and multiple test cross.The double low recessive gms line rapes of the homozygosis that the present invention selects, reproduction technique is simple, and outcrossing is good, and yield is high.
Description
Technical field
The invention belongs to rape breeding technique fields, and in particular to a kind of breeding of homozygous double low recessive gms line rapes
Method.
Background technique
Rape is the main oil crops in China, and for cultivated area more than 1.2 hundred million mu, China is maximum oil in the world
Dish producing region, rape are only second to the fifth-largest crop of paddy and wheat, corn and soybean in China.Rape is the work that " land used is increased soil fertility " combines
Object, not only contribute to increase soil fertility, and after rape stubble plant rice than after arrish plant rice can increase production 15% or so.From
Starting in 2007, country puts forth effort to cultivate the Yangtze river basin " double low " rape Pear dominant producing zone, at the same time, as rape production mechanization is broadcast
The popularization of kind technology and the maturation of mechanized harvest technology, it is contemplated that new rape variety in the Yangtze river basin there is wide popularization to answer
Use prospect.It carries out studying matched high-yield culture technique and comprehensive prevention and control of plant diseases, pest control skill by the cross-bred rape kind to tissue culture
Art carries out the unified production of hybrid seeds, implements to be widely applied.But existing rape variety hybrid seed yield is low, is not able to satisfy a large amount of
The demand of production.
Summary of the invention
The object of the present invention is to provide a kind of selections of homozygous double low recessive gms line rapes, select a kind of numerous
Plantation technology is simple, and outcrossing is good, the double low recessive gms line rapes of the high homozygosis of yield.
The present invention provides the following technical solutions：
A kind of selection of homozygous double low recessive gms line rapes, includes the following steps：
S1：Hybridized with cabbage type rape Huyouza 1 offspring sterile plant with Shanghai oil 15, obtains F1 generation；
S2：The F1 obtained in step S1 is hybridized with Shanghai oil 15, obtains BC1F1 generation；
S3：There is the more strain of sterile plant in selection in the BC1F1 generation obtained in step s 2, and selects plant type preferable
Fertile plant selfing obtains BC1F2 generation；
S4：The more strain of sterile plant is isolated in BC1F2 generation, and selects plant type is similar, economical character is excellent can
Strain and the pairs of test cross of sterile plant are educated, BC1F3 generation is obtained；
S5：The combination for selecting sterile plant more in BC1F3 generation carries out continuous several generation pairs of test crosses, final to obtain agronomy
Shape neat and consistent, Fertility segregation ratio stabilize to 1:The double low recessive gms line rapes of 1 homozygosis.
Preferably, the mode of fertile plant selfing is bagging selfing in the S3.
Preferably, the combination for selecting sterile plant more in BC1F3 generation in the S5 carries out continuous six generations pairs of test cross
The beneficial effects of the invention are as follows：
(1) selection of homozygous double low recessive gms line rapes provided by the invention, it is maternal to be easy with male parent,
Breeding is easy to operate.
(2) the double low recessive gms line rapes of homozygosis that the present invention selects, sterile plant petal are smaller compared with fertile plant, flat
Exhibition, stamen are degenerated completely, and filigree is not extended or slightly extended, the shrivelled no pollen of anther atrophy, sterile degree 100%, stamen development
Normally, outcrossing is good.
(3) the double low recessive gms line rapes of homozygosis that the present invention selects, reproduction technique is simple, and yield is high.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes part of specification, with reality of the invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings：
Fig. 1 is the flow diagram of the selection provided in the embodiment of the present invention.
Specific embodiment
One, as shown in Figure 1, a kind of selection of homozygous double low recessive gms line rapes, includes the following steps：
S1：2000, is hybridized with cabbage type rape Huyouza 1 offspring sterile plant with Shanghai oil 15, obtain F1 generation；
S2：2001, the F1 obtained in step S1 is hybridized with Shanghai oil 15, obtains BC1F1 generation；
S3：2002, there is the more strain of sterile plant in the BC1F1 obtained in step s 2 selection in, and selects strain
The preferable fertile plant bagging selfing of type, obtains BC1F2 generation；
S4：2003, the more strain of sterile plant is isolated in BC1F2 generation, and select that plant type is similar, economical character is excellent
Good fertile plant and the pairs of test cross of sterile plant, obtains BC1F3 generation；
S5：2004 to 2008, the combination for selecting sterile plant more in BC1F3 generation carried out continuous six generations pairs of test cross,
BC1F4 generation, BC1F5 generation, BC1F6 generation, BC1F7 generation, BC1F8 generation, final 2009 acquisition BC1F9 generations, i.e. agronomy are obtained year by year
Character neat and consistent, Fertility segregation ratio stabilize to 1:The double low recessive gms line rapes of 1 homozygosis, are denoted as " 024AB ".
Two, the main performance index for the double low recessive gms line rapes of homozygosis that the present embodiment breeding obtains is as follows：
1) tree characteristics：Seedling stage growing way is steady, and seedling partly crawls, and dark green leaf color, wax powder is medium, flower leaf type, and long handle leaf is
To raw decomposite leaf, 1-2 is to incising, Capping piece oblong.Petal foresythia, imbricate, seed dark brown.The time of infertility 225 days
Left and right, it is suitable with control.Average 155 centimetres of plant height, primary effectively branch amount 7-8, upper raw branching pattern, the effective silique of single plant
Number 260 or so, every seed number per pod 19 or so.
2) FLORAL CHARACTERS OF BIG：Sterile plant petal is smaller compared with fertile plant, open and flat, and stamen is degenerated completely, and filigree does not extend or slightly has
Elongation, the shrivelled no pollen of anther atrophy, sterile degree 100%, stamen development is normal, and outcrossing is good.
3) seed properties：Circle, black, 3.7 grams of mass of 1000 kernel or so.
Three, the propagation steps for the low recessive gms line rapes of homozygosis pair that the present embodiment breeding obtains include：
(1) the cultivation phase is broadcast：Sowing time is advisable with late September, is transplanted within seedling age 30 days or so, live streaming is postponed 10 days or so.
(2) Transplanting Density：It is advisable with 8000-10000 plants or so per acre.
(3) impurity elimination roguing：The plant of the Cruciferae such as spontaneous, Wild Rape and vegetables in breeding field is removed before facing the florescence
Strain；Different kind/dominant strain, variant in breeding field etc. was removed before the florescence, and hybrid strains and growing way are poor, plant is lopsided, disease pest
The weak plant of harm.
(4) fertile plant is extracted：Since facing the florescence, pulls out by determining row Beecher bottom and spent as in maternal row with normal
The fertile plant of powder, until in maternal row without normal fertile strain.
(5) field management：Stringent to select isolated area, heavy base fertilizer application on fertilizing management, early spraying fertilizer, top dressing is unsuitable too late,
Increase boron fertilizer；Prevent eliminating aphis early, in order to avoid virus infection；Maternal row sterile plant seed is harvested, per mu yield is 45 kilograms, in the production of hybrid seeds
It utilizes.
Field investigation is carried out in reproductive process, just the double low recessive gms line rapes of the homozygosis of flower, 2138 plants of total strain number,
992 plants of middle sterile plant, sterile plant rate 46.4%.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, although referring to aforementioned reality
Applying example, invention is explained in detail, for those skilled in the art, still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features.It is all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (3)
1. a kind of selection of homozygous double low recessive gms line rapes, which is characterized in that include the following steps：
S1：Hybridized with cabbage type rape Huyouza 1 offspring sterile plant with Shanghai oil 15, obtains F1 generation；
S2：The F1 obtained in step S1 is hybridized with Shanghai oil 15, obtains BC1F1 generation；
S3：There is the more strain of sterile plant in selection in the BC1F1 generation obtained in step s 2, and selects plant type preferably fertile
Strain selfing, obtains BC1F2 generation；
S4：The more strain of sterile plant is isolated in BC1F2 generation, and selects the fertile plant that plant type is similar, economical character is excellent
With the pairs of test cross of sterile plant, BC1F3 generation is obtained；
S5：The combination for selecting sterile plant more in BC1F3 generation carries out continuous several generation pairs of test crosses, and final acquisition economical character is whole
Homogeneous causes, and Fertility segregation ratio stabilizes to 1:The double low recessive gms line rapes of 1 homozygosis.
2. a kind of selection of homozygous double low recessive gms line rapes according to claim 1, which is characterized in that institute
The mode of fertile plant selfing in S3 is stated as bagging selfing.
3. a kind of selection of homozygous double low recessive gms line rapes according to claim 1, which is characterized in that institute
It states the combination for selecting sterile plant more in BC1F3 generation in S5 and carries out continuous six generations pairs of test cross.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810784751.5A CN108834879A (en) | 2018-07-17 | 2018-07-17 | A kind of selection of homozygous double low recessive gms line rapes |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810784751.5A CN108834879A (en) | 2018-07-17 | 2018-07-17 | A kind of selection of homozygous double low recessive gms line rapes |
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| CN108834879A true CN108834879A (en) | 2018-11-20 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1188594A (en) * | 1997-12-23 | 1998-07-29 | 安徽省农业科学院作物研究所 | Cabbage type rape recessive nueleus sterility triseries breeding and technology for production of seeds |
| CN103120118A (en) * | 2013-01-22 | 2013-05-29 | 贵州省油菜研究所 | Method for breeding cabbage type rape recessive epistasis interaction genic male sterility two type line |
| CN105830914A (en) * | 2016-04-14 | 2016-08-10 | 贵州粱丰农业科技有限公司 | Breeding method of cabbage-type rape high oil-content recessive epistatic interaction nuclear sterile homozygous two-type line |
| CN105850715A (en) * | 2016-04-14 | 2016-08-17 | 贵州粱丰农业科技有限公司 | Breeding method for cabbage-type rape high-oil-content recessive-epistatic-interaction nucleic-male-sterility homonuclear temporary maintaining line |
-
2018
- 2018-07-17 CN CN201810784751.5A patent/CN108834879A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1188594A (en) * | 1997-12-23 | 1998-07-29 | 安徽省农业科学院作物研究所 | Cabbage type rape recessive nueleus sterility triseries breeding and technology for production of seeds |
| CN103120118A (en) * | 2013-01-22 | 2013-05-29 | 贵州省油菜研究所 | Method for breeding cabbage type rape recessive epistasis interaction genic male sterility two type line |
| CN105830914A (en) * | 2016-04-14 | 2016-08-10 | 贵州粱丰农业科技有限公司 | Breeding method of cabbage-type rape high oil-content recessive epistatic interaction nuclear sterile homozygous two-type line |
| CN105850715A (en) * | 2016-04-14 | 2016-08-17 | 贵州粱丰农业科技有限公司 | Breeding method for cabbage-type rape high-oil-content recessive-epistatic-interaction nucleic-male-sterility homonuclear temporary maintaining line |
Non-Patent Citations (3)
| Title |
|---|
| 孙超才等: ""甘蓝型双低隐性核不育杂交种沪油杂1号的选育"", 《中国油料作物学报》 * |
| 王伟荣: ""甘蓝型双低油菜新品种的选育及示范推广"", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 韩宏仕: ""我国甘蓝型油菜隐性上位互作核不育三系的研究与利用"", 《贵州农业科学》 * |
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Application publication date: 20181120 |