CN105794509A - SNP chemical regulation method for increasing thermo-sensitive genic male sterile wheat BNS maturing rate - Google Patents
SNP chemical regulation method for increasing thermo-sensitive genic male sterile wheat BNS maturing rate Download PDFInfo
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- CN105794509A CN105794509A CN201610145552.0A CN201610145552A CN105794509A CN 105794509 A CN105794509 A CN 105794509A CN 201610145552 A CN201610145552 A CN 201610145552A CN 105794509 A CN105794509 A CN 105794509A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/24—Cyanogen or compounds thereof, e.g. hydrogen cyanide, cyanic acid, cyanamide, thiocyanic acid
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Abstract
The invention discloses an SNP chemical regulation method for increasing the thermo-sensitive genic male sterile wheat BNS maturing rate. The method comprises the steps that thermo-sensitive genic male sterile wheat BNS is sown on the fertile period sowing condition to form a fertile line, beginning from stamen development to the stamen and pistil primordium differentiation period till ears of wheat are just completely extracted from flag leaf sheaths, a sodium nitroprusside solution with the molar concentration being 1000 micromol/L is adopted for performing complete-plant spraying treatment on BNS plants every four days from the first day, the spraying amount of 50-80 L/mu, and other steps are executed referring to a common field production management mode. According to field production, after the sodium nitroprusside solution with the molar concentration being 1000 micromol/L is adopted for treatment, compared with natural growth control and clean water spraying control, the maturing rates obtained through statistics of the domestic law are improved by 26.61% and 29.58% respectively, and the maturing rates obtained through statistics of the international law are improved by 45.85% and 40.66% respectively. The method is relatively easy to implement, low in input cost and capable of saving time and labor and has the wide application prospect.
Description
Technical field
The invention belongs to temperature sensitive Genetic Sterility Semen Tritici aestivi BNS setting percentage chemical regulation technique field, be specifically related to a kind of raising temperature
The SNP chemical regulation method of quick Genetic Sterility Semen Tritici aestivi BNS setting percentage.
Background technology
The utilization of Semen Tritici aestivi photoperiod-temperature sensitive male sterility (PTSMS), is a major progress of hybrid wheat research.China finds
Semen Tritici aestivi photo-thermo-sensitive genetic male sterile line, such as types such as ES, C49S, ZP, BS, YS and BNS, it has also become hybrid wheat research important examination
Material.
Temperature sensitive Genetic Sterility Semen Tritici aestivi BNS, is cultivated successfully by eating the steel that shakes for 2002.BNS has sterile thorough and Fertility al-teration is thorough
The characteristic at the end, Sterility variation trend is: stamen abortion-half is sterile-can educate completely.Low temperature is affect BNS Fertility al-teration main
Reason, performance low temperature is sterile, and high temperature can be educated.It is the young fringe grown that BNS experiences the sensitive part of low temperature.BNS Fertility al-teration is sensitive
Phase is 5-18d before heading, i.e. the Pistil And Stamen idiophase forms the phase to tetrad.Within temperature sensitive period, when minimum temperature is less than 8 DEG C
Show the most sterile, form sterile line；8-12 DEG C is Fertility Transfer Temperature；During higher than 12 DEG C, performance can be educated completely, and formation can be educated
System is holding system.At north Henan, JIUYUE was sowed to October 17, is showed the most sterile on the 23rd, can be used for producing cenospecies；
After November 18, sowing all shows and can educate completely, can be with self propagated male-sterile seed.BNS sterile line is little at the Yellow River and Huai He River of China
Wheat main product regional representation sterility is good, conversion is easy, have specific restorative good characteristic, is a highly beneficial value
Can be used for the sterile line material of hybrid wheat.At north Henan, the direct planting type of open-land field is used to breed BNS seed money
Source, BNS is due to evening date of seeding under the conditions of can educating date of seeding, and compared with normal autumn sowing Common Wheat Varieties, general emergence rate is low, children's fringe
Differentiation and Floret development time are the shortest, and pollen fertility is on the low side, and setting percentage and yield are relatively low, it means that big of hybrid wheat
For breeding the relatively costly of BNS sterile line during the long-pending production of hybrid seeds.Therefore, improve BNS setting percentage under the conditions of can educating date of seeding,
Have great importance for reducing the breeding cost of hybrid wheat.
Producing and in scientific research at present, frequently with the method increasing planting percent, improving BNS under the conditions of can educating date of seeding
Yield.A kind of method is that first seed soaking promotes BNS Seed sprouting, and the smooth post-trenching of ploughing soil is poured water, and spreads after oozing under moisture
Broadcast, loosen the soil uniform fold about 3cm with jewelry, expensive clothing and other valuables the most again, its emergence rate, situation that planting percent is low are improved, but needs to plant
Son amount is big, and emergence rate, planting percent are the most relatively low.At present, BNS is many at temperature indoor cultivation under the conditions of can educating date of seeding, or in greenhouse
It is transplanted to open-land field cultivation after cultivation, or removing screen for hothouse changes near open big when temperature indoor cultivation is to certain state
Field is cultivated, and these 3 kinds of cultural methods are the highest to the requirement of facility, man power and material, relatively costly, it is difficult to meet the large area production of hybrid seeds
Requirement.There is also the interior possibility using covering with ground sheeting to improve emergence rate of after planting certain period of time, but this mode is due to right
The rising effect of ground temperature, can cause the quickening of BNS developmental process, is unfavorable for the performance of its normal fertility, at present on producing
Or in research work, there is not yet the application of this mode.Using above-mentioned cultural method, BNS all exists under the conditions of can educating date of seeding
The problem that setting percentage is relatively low.Normal autumn sowing can also be taked in theory to cultivate, but break up at the former base of its stamen development to Pistil And Stamen
After phase, start to proceed to greenhouse production, use plastic canopy insulation, till forming ripe three core pollen grains, especially exist
The Pistil And Stamen former base idiophase forms the phase to tetrad, it is ensured that when the lowest temperature is higher than 12 DEG C, and this becomes in the large area production of hybrid seeds produces
This is higher, it is difficult to popularization and application.Under the conditions of north Henan late sowing, allogenic material is used to improve BNS under the conditions of can educating date of seeding
The research of setting percentage, report is less at present, and utilizing sodium nitroprusside (SNP) to spray the process temperature sensitive Genetic Sterility Semen Tritici aestivi BNS of raising can educate
Under the conditions of date of seeding, the research of setting percentage has not yet to see report.
Summary of the invention
Present invention solves the technical problem that and there is provided a kind of SNP chemistry improving temperature sensitive Genetic Sterility Semen Tritici aestivi BNS setting percentage
Regulation and control method, so that BNS output increased under the conditions of can educating date of seeding, improves BNS seed production efficiency under the conditions of can educating date of seeding, protects
Hindering the wilderness demand to sterile line (normal autumn sowing) seed in the hybrid wheat large area production of hybrid seeds, the production of hybrid seeds reducing hybrid wheat becomes
This.
The present invention solves that above-mentioned technical problem adopts the following technical scheme that a kind of temperature sensitive Genetic Sterility Semen Tritici aestivi BNS of raising ties
The SNP chemical regulation method of real rate, it is characterised in that: temperature sensitive Genetic Sterility Semen Tritici aestivi BNS is sowed shape under can educating phase sowing condition
Become fertile line, from stamen development to the Pistil And Stamen former base idiophase, just extracting out from boot leaf sheath completely to the wheat head, from
Within first day, rise every 4 days once, using molar concentration is that the sodium nitroprusside solution of 1000 μm ol/L carries out Herb to BNS plant and sprays place
Reason, the amount of spraying is 50-80L/ mu, and other is with reference to general field production management mode, and wherein the Pistil And Stamen former base idiophase is specially
Plant the first internode elongation to 0.9-1.1cm, circular gynoecium in the middle part of first little Hua of small ear in the middle part of basis of microscopic observation to fringe
Former base and three peripheral circular protrusions shapes and the stamen retrogressive without miniature longitudinal furrow, the time that sprays of sodium nitroprusside solution is the same day
18:00-19:00。
Further preferably, described educated phase sowing condition particularly as follows: the Yellow River and Huai He River Semen Tritici aestivi main product area by temperature sensitive Genetic Sterility
Semen Tritici aestivi BNS is sowing after November 18 then.
Further preferably, described educated phase sowing condition particularly as follows: the Yellow River and Huai He River Semen Tritici aestivi main product area by temperature sensitive Genetic Sterility
Semen Tritici aestivi BNS is in December sowing on the 2nd then.
The present invention uses chemicals that BNS plant under the conditions of can educating date of seeding is carried out spraying treatment, so can make up
The defect of temperature control technology, improves BNS setting percentage more easily, is not the strictest to facility requirements.Use the side of the present invention
Method can exempt nurse young plants in hothouses, transplant, the tedious work such as flip greenhouse, human input is less.
BNS plant under the conditions of can educating date of seeding is entered by the sodium nitroprusside solution that the present invention selects molar concentration to be 1000 μm ol/L
Row sprays process, only referring to general field production management mode.The sodium nitroprusside using molar concentration to be 1000 μm ol/L is molten
After liquid processes, compared with the comparison naturally grown and spraying the comparison of clear water；The setting percentage of domestic law statistics is respectively increased
26.61% and 29.58%, the setting percentage of international law statistics has been respectively increased 45.95% and 40.66%.At present, obtained by test
Obtained successfully.The present invention operates relatively easy, and thrown cost is relatively low, time saving and energy saving, the side of regulation and control BNS fertility restorer more originally
Method is easy, has broad application prospects.
Detailed description of the invention
By the following examples the foregoing of the present invention is described in further details, but this should be interpreted as this
The scope inventing above-mentioned theme is only limitted to below example, and all technology realized based on foregoing of the present invention belong to this
Bright scope.
Embodiment 1
With temperature sensitive Genetic Sterility Semen Tritici aestivi BNS as test material, December in 2013 sowing on the 2nd, i.e. form BNS late sowing and can educate holding system
(keeping system to represent with BNS).Test site is He'nan Normal University's experimental field of wheat, and soil is sandy loam, 0-30cm soil layer
Soil is containing organic 10.7g/kg, full nitrogen 0.46g/kg, alkali-hydrolyzable nitrogen 60.1mg/kg, rapid available phosphorus 31.1mg/kg, available potassium
92.1mg/kg.Plot area is 1.5m × 3m(4.5m2), line-spacing 24cm, spacing in the rows 10cm(uses intensive broadcasting sowing, after emerging again
The method of final singling determines spacing in the rows).The group arrangement of random district, is repeated 3 times.Dried poultrymanure 1.7t/ mu, Stanley are executed before ploughing in each community
(three peaces) complex fertilizer 50kg/ mu, N-P2O5-K2O=18:18:18, total nutrient >=54%, N-P2O5-K2The mu consumption of O is 9kg,
It is respectively cooperating with watering in jointing stage and boot stage and imposes Stanley (three peaces) complex fertilizer each 25kg/ mu, N-P2O5-K2The mu of O is used
Amount is respectively 4.5kg, and Stanley chemical fertilizer (Plain) company limited produces.In kernel grouting initial stage i.e. Post flowering 15d, supplement blade face
Fertilizer is 12.5g/L(net content 1.238 × 10g/L) potassium dihydrogen phosphate, potassium dihydrogen phosphate is controlled interest limited public affairs by Shandong Ke Saijinong
Department produces, KH2PO4>=99%, K2O >=34%, P2O5≥52%.From temperature sensitive Genetic Sterility Semen Tritici aestivi BNS under the conditions of can educating date of seeding, it is male
Stamen is developed to the Pistil And Stamen former base idiophase and (fringe in the middle part of 3-4 be can be observed under plant the first internode elongation to about 1cm, microscope
The former base of circular gynoecium in the middle part of first little Hua of individual small ear is former with peripheral three circular protrusions shapes and the stamen without miniature longitudinal furrow
Base) start, just extracting out from boot leaf sheath completely to the wheat head, from first day every 4 days once, use 1000 μm ol/L
Sodium nitroprusside solution carries out Herb to BNS plant under the conditions of can educating date of seeding and sprays process.To be naturally grown to compare 1, clear with spray
Water is comparison 2.All spray measure include compare 2(clear water) and 1000 μm ol/L sodium nitroprusside solution all with 5.0mL/L tween-
80 is adhesion promoter, in order to its adhesion on blade；The amount of spraying is 50-80L/ mu, carries out in dusk 18:00-19:00.
Every furrow zone line (not drawing materials in furrow two ends and both sides), often process bagging 10 strain before blooming after heading, every strain
Set stem fringe, measures its setting percentage and averages during results.Use domestic law and international law statistics setting percentage simultaneously.Setting percentage meter
Calculation formula is respectively as follows:
Setting percentage (%)=(base portion little bow structure real number/spikelet number × 2) × 100%(domestic law)
Setting percentage (%)=(grain number per spike/spikelet number × 2) × 100%(international law)
BNS late sowing can be educated holding line fertility measurement result and is shown in Table 1.
Table 1 temperature sensitive Genetic Sterility Semen Tritici aestivi BNS fertility under different disposal method under the conditions of can educating date of seeding compares
Note: the upper and lower case letter after data represents the same column of figure significance of difference in 0.01 and 0.05 level respectively.
Experiment proves: with reference to general field production management mode, molar concentration is at the sodium nitroprusside solution of 1000 μm ol/L
Reason can improve temperature sensitive Genetic Sterility Semen Tritici aestivi BNS setting percentage under the conditions of can educating date of seeding, thus proves this regulation and control Semen Tritici aestivi fertility
New technique can actually improve its strain breeding and seed breeding efficiency, advantageously reduce the breeding cost of hybrid wheat, compensate for temperature adjust
Control the shortcoming of its fertility poor effect.
It should be noted last that, above example is only in order to illustrate, and unrestricted technical scheme, although ginseng
According to above-described embodiment, the present invention is described in detail, it will be understood by those within the art that: still can be to this
Invention is modified or equivalent, and any modification or partial replacement without departing from the spirit and scope of the present invention, it is equal
Should contain in the middle of scope of the presently claimed invention.
Claims (3)
1. the SNP chemical regulation method improving temperature sensitive Genetic Sterility Semen Tritici aestivi BNS setting percentage, it is characterised in that: by temperature sensitive core not
Educate Semen Tritici aestivi BNS and under can educating phase sowing condition, sow formation fertile line, from stamen development to the Pistil And Stamen former base idiophase, extremely
Till the wheat head is just extracted out completely from boot leaf sheath, within every 4 days from first day, once using molar concentration is 1000 μm ol/L
Sodium nitroprusside solution carries out Herb to BNS plant and sprays process, and the amount of spraying is 50-80L/ mu, and other is with reference to general field production pipe
Reason pattern, wherein the Pistil And Stamen former base idiophase is specially plant the first internode elongation to 0.9-1.1cm, and basis of microscopic observation is to fringe
The former base of circular gynoecium in the middle part of first little Hua of small ear of middle part and three peripheral circular protrusions shapes and the stamen without miniature longitudinal furrow
Former base, the 18:00-19:00 that the time that sprays is the same day of sodium nitroprusside solution.
The SNP chemical regulation method of raising the most according to claim 1 temperature sensitive Genetic Sterility Semen Tritici aestivi BNS setting percentage, its feature
Be described educated phase sowing condition particularly as follows: the Yellow River and Huai He River Semen Tritici aestivi main product area by temperature sensitive Genetic Sterility Semen Tritici aestivi BNS in then 11
Sowing after the moon 18.
The SNP chemical regulation method of raising the most according to claim 1 temperature sensitive Genetic Sterility Semen Tritici aestivi BNS setting percentage, its feature
Be described educated phase sowing condition particularly as follows: the Yellow River and Huai He River Semen Tritici aestivi main product area by temperature sensitive Genetic Sterility Semen Tritici aestivi BNS in then 12
The moon 2 sowing.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108998556A (en) * | 2018-08-29 | 2018-12-14 | 北京市农林科学院 | A kind of SNP marker and its application identified or assist identification Chinese cabbage crossing setting percentage |
| CN112703981A (en) * | 2021-01-06 | 2021-04-27 | 安徽农业大学 | Method for relieving wheat photosynthetic performance loss under high-temperature stress |
| CN114830936A (en) * | 2022-04-24 | 2022-08-02 | 河南师范大学 | Method for thoroughly sterilizing pollen of different fertility groups of BNS series temperature-sensitive genic sterile wheat |
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| CN103609351A (en) * | 2013-12-10 | 2014-03-05 | 河南师范大学 | Method for regulating chrysanthemum flowering phase |
| CN103650890A (en) * | 2013-12-24 | 2014-03-26 | 河南师范大学 | Method for improving yield of peanuts |
| CN104620880A (en) * | 2015-02-02 | 2015-05-20 | 河南师范大学 | Chemical control method for facilitating BNS maintenance line fertility restoration |
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| CN103609351A (en) * | 2013-12-10 | 2014-03-05 | 河南师范大学 | Method for regulating chrysanthemum flowering phase |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108998556A (en) * | 2018-08-29 | 2018-12-14 | 北京市农林科学院 | A kind of SNP marker and its application identified or assist identification Chinese cabbage crossing setting percentage |
| CN112703981A (en) * | 2021-01-06 | 2021-04-27 | 安徽农业大学 | Method for relieving wheat photosynthetic performance loss under high-temperature stress |
| CN114830936A (en) * | 2022-04-24 | 2022-08-02 | 河南师范大学 | Method for thoroughly sterilizing pollen of different fertility groups of BNS series temperature-sensitive genic sterile wheat |
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