CN105794451B - Promote the method for the thorough abortion of temperature sensitive Genetic Sterility wheat BNS sterile line pollen using SNP - Google Patents
Promote the method for the thorough abortion of temperature sensitive Genetic Sterility wheat BNS sterile line pollen using SNP Download PDFInfo
- Publication number
- CN105794451B CN105794451B CN201610145550.1A CN201610145550A CN105794451B CN 105794451 B CN105794451 B CN 105794451B CN 201610145550 A CN201610145550 A CN 201610145550A CN 105794451 B CN105794451 B CN 105794451B
- Authority
- CN
- China
- Prior art keywords
- bns
- wheat
- sterile line
- temperature sensitive
- genetic sterility
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/24—Cyanogen or compounds thereof, e.g. hydrogen cyanide, cyanic acid, cyanamide, thiocyanic acid
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Forests & Forestry (AREA)
- Ecology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Zoology (AREA)
- Pest Control & Pesticides (AREA)
- Inorganic Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of methods for promoting the thorough abortion of temperature sensitive Genetic Sterility wheat BNS sterile line pollen using SNP, temperature sensitive Genetic Sterility wheat BNS is sowed under sterile phase sowing condition and forms sterile line, since stamen development to Pistil And Stamen former base idiophase, until the wheat head is just extracted out completely from boot leaf leaf sheath, from first day every 4 days it is primary, molar concentration is used to carry out complete stool sprays processing to BNS plant for the sodium nitroprusside solution of 200 μm of ol/L, the amount of spraying is 50-80L/ mus, other general field production management modes of reference.The present invention passes through field production, use molar concentration for the processing of the SNP solution of 200 μm of ol/L, to make BNS sterile line pollen abortion rate reach complete sterility level, self-fruitful rate is 0%, reach requirement to sterile line in hybrid wheat production, operate relatively easy, throws that cost is relatively low, it is time saving and energy saving, it has broad application prospects.
Description
Technical field
The invention belongs to temperature sensitive Genetic Sterility wheat BNS pollen fertility chemical regulation technique fields, and in particular to a kind of utilization
The method that SNP promotes the thorough abortion of temperature sensitive Genetic Sterility wheat BNS sterile line pollen.
Background technique
The utilization of wheat photoperiod-temperature sensitive male sterility (PTSMS) is a major progress of hybrid wheat research.China's discovery
Wheat photo-thermo-sensitive genetic male sterile line, such as ES, C49S, ZP, BS, YS and BNS type, it has also become the important examination of hybrid wheat research
Material.
Temperature sensitive Genetic Sterility wheat BNS is cultivated successfully for steel 2002 by eating vibration, and BNS has infertility thoroughly and Fertility al-teration is thorough
The characteristic at bottom, Sterility variation trend are as follows: stamen abortion-semisterility-is completely fertile.Low temperature is the main of influence BNS Fertility al-teration
Reason, performance low temperature infertility, high temperature are fertile.The sensitive part that BNS experiences low temperature is the young fringe developed.BNS Fertility al-teration is sensitive
Phase 5-18d before heading, i.e. Pistil And Stamen idiophase to tetrahedron are at the phase, within temperature sensitive period, when minimum temperature is lower than 8 DEG C
Performance is thoroughly sterile, forms sterile line；8-12 DEG C is Fertility Transfer Temperature；Performance is completely fertile when higher than 12 DEG C, is formed fertile
System is to keep system.In north Henan, was sowed to October 17 within September 23rd, show thoroughly infertility, can be used for producing cenospecies；
It is completely fertile that performance is sowed after November 18, it can be with self propagated male-sterile seed.The Yellow River and Huai He River of BNS sterile line in China is small
Wheat main product regional representation sterility is good, conversion is easy, has specific restorative good characteristic, is a highly beneficial value
It can be used for the sterile line material of hybrid wheat.In BNS Fertility al-teration sensitive periods, often there is lowest temperature cyclic fluctuation,
There is the case where being higher than 8 DEG C in certain section of time lowest temperature, and fertility stability is also interfered and fluctuates, by thoroughly infertility
There is part fertility restorer, part fertile flower powder can be detected, self-fruitful rate is also gone up, and it is miscellaneous that this can reduce seed farm
The purity for handing over seed will be that one in large area production application of two-line-method hybrid wheat potentially seriously threatens.
Overcome lowest temperature cyclic fluctuation to the basic countermeasure that BNS fertility stability interferes theoretically exist it is a variety of can
Energy.One kind may be cooperation wheat to photoperiodic sensitivity, be bred as the Dual Sensitive core sensitive to photoperiod and low temperature not
It educates and is, but this needs very long scientific research process just and has the possibility of realization, be only limitted to theory analysis to this at present, in practice still
It may without realizing.Alternatively possible is to be increased using agricultural modernization facility control Short-range Temperature, but this is higher to facility requirements,
It is difficult to meet the needs of large area hybrid seeding.It has been bullied thermoperiodicity influence of fluctuations in Fertility al-teration sensitive periods, or prediction meeting
In the case of temperature cyclic fluctuation occur and influencing, the same period auxiliary suitable chemical regulation measure gametocide can also be used as a kind of benefit
Measure is rescued to improve seed farm double-line hybrid seed purity.Forefathers' research points out that the existing male sterilant such as ethephon (CEPHA),2-(chloroethyl) phosphonic acid can reach
To the effect of effective gametocide, but there are wheat flower development processes to slow down after for ethephon (CEPHA),2-(chloroethyl) phosphonic acid, and heading is difficult, be easy it is remaining green when it is due to become yellow and ripe late-maturing,
It is easy the infection problems such as pest and disease damage, other existing male sterilants exist big to wheat plant toxicity more, reduce male and educate
While property, also there is strong depression effect to Pistil Fertility, setting percentage is low after artificial supplementary pollination and hollow kernels, drug
Costco Wholesale is higher, thus limits application of the existing male sterilant in production, and is used for using existing chemical hybridizing agent
The cyclic fluctuation of Fertility al-teration sensitive periods temperature is overcome to grind temperature sensitive Genetic Sterility wheat BNS sterile line fertility stability interference
Study carefully, report at present less, sprays processing using sodium nitroprussiate (SNP) and promote the research mesh of the thorough abortion of wheat BNS sterile line pollen
It is preceding that there is not been reported.For temperature sensitive Genetic Sterility wheat BNS sterile line, and female fertility larger to male fertile reduction degree is screened
Reduction amplitude is little, and cheap new chemical hybridization agent, for promoting BNS sterile line thoroughly sterile, for it miscellaneous
The effective use in the wheat production of hybrid seeds is handed over, breeding cost is reduced and has great importance.
Summary of the invention
Promote temperature sensitive Genetic Sterility wheat BNS sterile line flower using SNP the technical problem to be solved by the present invention is to provide a kind of
The method of the thorough abortion of powder so that BNS sterile line infertility is thoroughly, and then ensures the purity and seed production efficiency of hybrid seed, reduces
The breeding cost of hybrid wheat.
The present invention adopts the following technical scheme that solve above-mentioned technical problem promotes temperature sensitive Genetic Sterility wheat BNS using SNP
The method of the thorough abortion of sterile line pollen, it is characterised in that: sow temperature sensitive Genetic Sterility wheat BNS under sterile phase sowing condition
Sterile line is formed, since stamen development to Pistil And Stamen former base idiophase, until the wheat head is just extracted out completely from boot leaf leaf sheath,
Once using molar concentration is that the sodium nitroprusside solution of 200 μm of ol/L carries out complete stool sprays to BNS plant within every 4 days from first day
Processing, the amount of spraying are 50-80L/ mus, and other general field production management modes of reference, wherein the Pistil And Stamen former base idiophase is specific
For the first internode elongation of plant to 0.9-1.1cm, the circle in the middle part of microscopically observation to fringe in the middle part of first little Hua of small ear is female
Three circular protrusions shapes of stamen former base and periphery and the stamen retrogressive without miniature longitudinal furrow, the spraying time of sodium nitroprusside solution are the same day
18:00-19:00.
Further preferably, the sterile phase sowing condition specifically: in the Yellow River and Huai He River wheat main product area by temperature sensitive Genetic Sterility
Wheat BNS was sowed in current year September 23rd to October 17.
It further limits, the sterile phase sowing condition specifically: in the Yellow River and Huai He River wheat main product area by temperature sensitive Genetic Sterility
Wheat BNS is sowed October 13 current year.
The present invention carries out spraying treatment to BNS plant using chemicals, can make up for it lacking for temperature control technology in this way
It falls into, more easily regulates and controls BNS fertility, make its thorough abortion of sterile line pollen, self-fruitful rate zero reaches producing hybrid wheat seed
Requirement to sterile line is not very stringent to facility requirements.Using method of the invention can exempt control temperature to facility and
Artificial requirement, less input.
It is that the SNP solution of 200 μm of ol/L carries out BNS plant to spray processing that the present invention, which selects molar concentration, only referring to one
As field production management mode.By field production, use molar concentration for the processing of the SNP solution of 200 μm of ol/L, thus
BNS sterile line pollen abortion rate is set to reach complete sterility level, self-fruitful rate 0% reaches in hybrid wheat production to sterile line
Requirement, currently, passed through test obtain success.Operation of the present invention is relatively easy, throws that cost is relatively low, time saving and energy saving,
It has broad application prospects.
Specific embodiment
Above content of the invention is described in further details by the following examples, but this should not be interpreted as to this
The range for inventing above-mentioned theme is only limitted to embodiment below, and all technologies realized based on above content of the present invention belong to this hair
Bright range.
Embodiment 1
Using temperature sensitive Genetic Sterility wheat BNS as test material, on October 13rd, 2013, sowing, that is, formed BNS sterile line.Test
Place is He'nan Normal University's experimental field of wheat, and soil is sandy loam, the soil 10.7g/kg containing organic matter of 0-30cm soil layer, entirely
Nitrogen 0.46g/kg, alkali-hydrolyzable nitrogen 60.1mg/kg, rapid available phosphorus 31.1mg/kg, available potassium 92.1mg/kg.Plot area is 1.5 m
× 3m(4.5m2), line-spacing 24cm, spacing in the rows 10cm(are first broadcast using lower than 10cm spacing in the rows pendulum, fixed by 10cm spacing in the rows again after emerging
Seedling).Random district's groups arrangement, is repeated 3 times.Each cell applies 1.7t/ mus of dried poultrymanure before arable land, Stanley (three peaces) complex fertilizer
50kg/ mus, N-P2O5-K2O=18:18:18, total nutrient >=54 %, N-P2O5-K2The mu dosage of O is 9kg, in jointing stage and booting
Phase is respectively cooperating with watering 25kg/ mus each, the N-P that imposes Stanley (three peace) complex fertilizer2O5-K2The mu dosage of O is respectively 4.5kg,
The production of Stanley chemical fertilizer (Plain) Co., Ltd.In kernel grouting initial stage, that is, Post flowering 15d, supplementing foliar fertilizer is that 12.5g/L(is net
1.238 × 10g/L of content) potassium dihydrogen phosphate, potassium dihydrogen phosphate is by the production of Shandong Ke Saijinong Pty Ltd, KH2PO4≥
99 %, K2O >=34 %, P2O5≥52 %.Divide from temperature sensitive Genetic Sterility wheat BNS early sowing sterile line stamen development to Pistil And Stamen former base
The change phase, (first little Hua of 3-4 small ear in the middle part of fringe can be observed under microscope for the first internode elongation of plant to 0.9-1.1cm
The round gynoecium former base at middle part and peripheral three circular protrusions shapes and the stamen retrogressive without miniature longitudinal furrow) start, until the wheat head is just complete
Extract out and stop from boot leaf leaf sheath entirely, from first day every 4 days it is primary, use molar concentration for the SNP solution pair of 200 μm of ol/L
BNS sterile line plant carries out complete stool sprays processing.To be grown to control 1 naturally, to spray clear water for control 2.It is all to spray measure
Including compare 2(clear water) and molar concentration be 200 μm of ol/L SNP solution spray processing with 5.0mL/L Tween-80 for help it is viscous
Agent, so as to its adherency on blade, the amount of spraying is 50-80L/ mus, is carried out in dusk 18:00-19:00.
It being observed and recorded according to stamen development process, BNS sterile line enters the Pistil And Stamen idiophase on March 16th, 2014, until
There is tetrad on March 30th, 2014, is its Fertility al-teration sensitive periods during this, and the lowest temperature is shown as 6 DEG C (3 by day record
Months 16 days), 8 DEG C (March 17), 7 DEG C (March 18), 5 DEG C (March 19), 4 DEG C (March 20), 5 DEG C (March 21), 9 DEG C
(March 22), 10 DEG C (March 23), 9 DEG C (March 24), 12 DEG C (March 25), 12 DEG C of (March 26), 15 DEG C of (March 27
Day), 13 DEG C (March 28), 13 DEG C (March 29), 12 DEG C (March 30), i.e., the Fertility al-teration for continuing 15 days in BNS sterile line
In sensitive periods, although mean minimum temperature be 7.9 DEG C, be BNS fertility performance thoroughly infertility temperature, wherein have 7 days it is minimum
Temperature is 8-12 DEG C, is Fertility Transfer Temperature, and the lowest temperature is higher than 12 DEG C for three days on end from after March 27, is that fertility performance is complete
Fertile temperature, this lowest temperature fluctuation disturb the stability of BNS infertility line fertility, and self-fruitful rate also has to a certain degree
Go up, during carrying out hybrid wheat breeding using BNS, is unfavorable for ensureing the purity of hybrid wheat seed.
Every furrow intermediate region (furrow both ends and both sides are not drawn materials), every processing took 10 same day that will bloom in the early flowering season
Tassel, to peel off middle part small ear the 1st manually, see anther after 2 little Hua as yellow and filigree is extended to will stretch out but not yet stretch
Out subject to glume.Every fringe is a sample, takes middle part small ear respectively from bottom to top, every 1, take totally 3 pieces of 1 piece of anther in 2 little Hua,
It squeezes out all pollen in anther as far as possible on glass slide, after removing anther wall fragment, is contaminated with the I-KI that mass concentration is 1%
Color, microscopically observation fertility.Microscopy counts normal fertile pollen rate and abortive pollen grain, with 5 visuals field be averaged for
One measured value simultaneously seeks pollen abortion rate.It is final that 10 measured values obtained by 10 fringes and gained pollen abortion rate are averaged again
Measured value.
Abortion rate (%)=abortive pollen grain number/(normal pollen grain number+abortive pollen grain number) × 100%.
Every furrow intermediate region (furrow both ends and both sides are not drawn materials), 10 plants of sets are taken at random in each area before blooming after heading
Bag, every plant of set stem fringe, when harvest, measure its setting percentage and average；10 plants are taken at random in each area simultaneously, every plant of set stem
Fringe is carried out manually being saturated pollination with 18 pollen of common wheat week wheat, measurement saturation pollination setting percentage；Each area's (furrow two when mature
End and both sides are not drawn materials) 10 plants of non-bagging plant stem fringes are received at random, measure outcrossing seed-setting rate.Internal law and the world are used simultaneously
Legally constituted authority meter setting percentage.Setting percentage calculation formula is respectively as follows:
Setting percentage (%)=(solid number/spikelet number × 2 base portion little Hua) × 100%(internal law)
Setting percentage (%)=(grain number per spike/spikelet number × 2) × 100%(international law)
BNS infertility line fertility measurement result is shown in Table 1.
Fertility of the temperature sensitive Genetic Sterility wheat BNS sterile line of table 1 under different disposal method compares
Note: the upper and lower case letter after data respectively indicates the significance of difference of the same column of figure in 0.01 and 0.05 level.
Experiments have shown that: referring to general field production management mode, the SNP solution that molar concentration is 200 μm of ol/L handles energy
Enough increase temperature sensitive Genetic Sterility wheat BNS sterile line pollen abortion rate reach 100% complete sterility level, self-fruitful rate 0%,
And with control 2(clear water) compared with, decline to a certain degree though saturation pollination setting percentage and outcrossing seed-setting rate decline have, but still can tie up
Certain level is held, to prove that this new technology for promoting the thorough abortion of temperature sensitive Genetic Sterility wheat BNS sterile line pollen really can
Enough overcome temperature to fluctuate the interference to its fertility, keeps its thoroughly infertility, ensure the purity and seed production efficiency of hybrid seed, favorably
In the breeding cost and risk that reduce hybrid wheat.
Embodiment above describes basic principles and main features of the invention and advantages.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe originals of the invention
Reason, under the range for not departing from the principle of the invention, various changes and improvements may be made to the invention, these changes and improvements are each fallen within
In the scope of protection of the invention.
Claims (3)
1. promoting the method for the thorough abortion of temperature sensitive Genetic Sterility wheat BNS sterile line pollen using SNP, it is characterised in that: will be temperature sensitive
Genetic Sterility wheat BNS is sowed under sterile phase sowing condition and is formed sterile line, is opened from stamen development to the Pistil And Stamen former base idiophase
Begin, until the wheat head is just extracted out completely from boot leaf leaf sheath, from first day every 4 days it is primary, use molar concentration for 200 μ
The sodium nitroprusside solution of mol/L carries out complete stool sprays processing to BNS plant, and the amount of spraying is 50-80L/ mus, wherein Pistil And Stamen former base point
The change phase is specially the first internode elongation of plant to 0.9-1.1cm, in the middle part of microscopically observation to fringe in the middle part of first little Hua of small ear
Round gynoecium former base and periphery three circular protrusions shapes and the stamen retrogressive without miniature longitudinal furrow, when spraying of sodium nitroprusside solution
Between be the same day 18:00-19:00.
2. the side according to claim 1 for promoting the thorough abortion of temperature sensitive Genetic Sterility wheat BNS sterile line pollen using SNP
Method, it is characterised in that the sterile phase sowing condition specifically: in the Yellow River and Huai He River wheat main product area by temperature sensitive Genetic Sterility wheat BNS
It was sowed in current year September 23rd to October 17.
3. the side according to claim 1 for promoting the thorough abortion of temperature sensitive Genetic Sterility wheat BNS sterile line pollen using SNP
Method, it is characterised in that the sterile phase sowing condition specifically: in the Yellow River and Huai He River wheat main product area by temperature sensitive Genetic Sterility wheat BNS
It is sowed October 13 current year.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610145550.1A CN105794451B (en) | 2016-03-15 | 2016-03-15 | Promote the method for the thorough abortion of temperature sensitive Genetic Sterility wheat BNS sterile line pollen using SNP |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610145550.1A CN105794451B (en) | 2016-03-15 | 2016-03-15 | Promote the method for the thorough abortion of temperature sensitive Genetic Sterility wheat BNS sterile line pollen using SNP |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105794451A CN105794451A (en) | 2016-07-27 |
| CN105794451B true CN105794451B (en) | 2019-02-22 |
Family
ID=56468473
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610145550.1A Active CN105794451B (en) | 2016-03-15 | 2016-03-15 | Promote the method for the thorough abortion of temperature sensitive Genetic Sterility wheat BNS sterile line pollen using SNP |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105794451B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114830936B (en) * | 2022-04-24 | 2023-07-21 | 河南师范大学 | Method for thoroughly sterility of pollen of different fertility populations of BNS series thermosensitive genic male sterile wheat |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104641968A (en) * | 2015-02-02 | 2015-05-27 | 河南科技学院 | Chemical regulating method for keeping sterile line of thermo-sensitive genic male sterile wheat BNS thoroughly aborted |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5614647B2 (en) * | 2009-01-08 | 2014-10-29 | 国立大学法人東北大学 | Method for recovering male sterility of gramineous plants and male sterility recovery agent |
-
2016
- 2016-03-15 CN CN201610145550.1A patent/CN105794451B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104641968A (en) * | 2015-02-02 | 2015-05-27 | 河南科技学院 | Chemical regulating method for keeping sterile line of thermo-sensitive genic male sterile wheat BNS thoroughly aborted |
Non-Patent Citations (3)
| Title |
|---|
| 一氧化氮参与胞外ATP抑制泡桐花粉萌发和花粉管伸长;张媛华 等;《陕西师范大学学报》;20110930;第39卷(第5期);第62-66页 |
| 低温对茶树花粉管抑制作用与NO关系的研究;王伟东 等;《园艺学报》;20131231;第40卷(第8期);第1535-1540页 |
| 植物中的NO及其对花发育的调节;周坤 等;《遗传》;20140731;第36卷(第7期);第661-668页 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105794451A (en) | 2016-07-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Dai et al. | Intensive cotton farming technologies in China: Achievements, challenges and countermeasures | |
| Zhang et al. | An improved water-use efficiency for winter wheat grown under reduced irrigation | |
| CN103598051B (en) | Compartment furrow fixed three-surround three-dimensional intensive cultivation method for rice | |
| CN103988773B (en) | Indica Hybrid Rice combines 6 liang of excellent producing method for seed of No. 2 | |
| CN104137724B (en) | Wild cabbage 1 year two Ji Sishou cultivation method are overlapped between set course glutinous sweet corn, summer Autumn Chinese cabbage between garlic | |
| Villa et al. | Hybrid rice performance in environments of increasing drought severity | |
| CN102090316A (en) | Method for producing rice hybrid Guangliangyou 206 | |
| CN101919351A (en) | Production method of hybrid rice | |
| CN106576727A (en) | Pollution-free sunflower culture method | |
| CN105165340A (en) | Greenhouse strawberry planting method | |
| CN107581009A (en) | A kind of corn soybean Intercropping Cultivation method | |
| CN103416206A (en) | Improved variety breeding technology for industrial cannabis sativa L. | |
| CN106717826A (en) | The implantation methods of corn with high yield | |
| CN108633669B (en) | Method for cultivating medium-ripened trailing short cowpea variety in south China in open field in summer | |
| CN104641968B (en) | Chemical regulating method for keeping sterile line of thermo-sensitive genic male sterile wheat BNS thoroughly aborted | |
| CN104620880B (en) | Promote that temperature sensitive Genetic Sterility Semen Tritici aestivi BNS keeps the chemical regulation method that line fertility recovers | |
| CN105230305A (en) | Cultivation method with high photosynthetic efficiency for crops having concave and convex canopies | |
| CN102090263A (en) | Method for producing Guangliangyou No.1 hybrid rice seeds | |
| CN102090315A (en) | Method for producing hybrid rice seeds Chuanxiangzhan | |
| CN107041224B (en) | Composite planting method for planting water spinach and towel gourd in rice field | |
| CN105794509A (en) | SNP chemical regulation method for increasing thermo-sensitive genic male sterile wheat BNS maturing rate | |
| CN109328717A (en) | A method of promote B. campestris L.ssp. Chinensis to bloom ahead of time | |
| CN105794451B (en) | Promote the method for the thorough abortion of temperature sensitive Genetic Sterility wheat BNS sterile line pollen using SNP | |
| CN107155447A (en) | A kind of method of shallot fast breeding | |
| CN105028173B (en) | A kind of cross breeding method of phosphorus efficiency corn |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |