CN103733976A - Method for breeding male sterile line by use of sterile cytoplasm of hibiscus cannabinus KN142 - Google Patents
Method for breeding male sterile line by use of sterile cytoplasm of hibiscus cannabinus KN142 Download PDFInfo
- Publication number
- CN103733976A CN103733976A CN201310711047.4A CN201310711047A CN103733976A CN 103733976 A CN103733976 A CN 103733976A CN 201310711047 A CN201310711047 A CN 201310711047A CN 103733976 A CN103733976 A CN 103733976A
- Authority
- CN
- China
- Prior art keywords
- male
- sterile
- bluish dogbane
- male sterile
- cytoplasm
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for breeding a male sterile line by use of the sterile cytoplasm of hibiscus cannabinus KN142, which specifically comprises the following steps: performing saturation backcross by taking a breeding material containing the sterile cytoplasm of hibiscus cannabinus KN142 as a female parent and the cultivated variety of hibiscus cannabinus as a male parent, and selecting a uniform wild abortive type cytoplasmic male sterile line similar to the male parent in character and showing male sterility, wherein the male parent for backcross is a corresponding male sterility maintainer line. The method disclosed by the invention has the beneficial effects that the hybrid matched with the KN142 cytoplasmic male sterile line and the hybrid matched with the UG93 or H040 cytoplasmic male sterile line are popularized and utilized at the same time, and the occurrence risk of certain disease can be greatly reduced.
Description
Technical field
The present invention relates to crop breeding method, specifically utilize the method for the sterile cytoplasm seed selection male sterile line of bluish dogbane KN142.
Background technology
Bluish dogbane claims again " mestha ", is the annual phloem fiber crop of Malvaceae Hibiscus.Because of bluish dogbane, take results phloem fiber or stem stalk is cultivation object, and heterosis rate is up to 35%～40%, and bluish dogbane heterosis utilization is the main goal of attack of domestic and international bluish dogbane breeding for a long time.Hemp Inst., China Academy of Agricultural Sciences, through research for many years, has invented bluish dogbane chemical emasculation cross breeding method, and has applied in bluish dogbane breeding.But because bluish dogbane is indefinite inflorescence, need repeatedly dispenser, and after dispenser, affected insect pollination, need artificial pollination.For cost-saving, have to promote aborning F
2generation (second filial generation).But F
2irregular for plant, heterosis rate obviously reduces, therefore the Kenaf Hybrid H area of promoting in bluish dogbane produces is so far very limited.Therefore, seed selection bluish dogbane cytoplasmic male sterile line is to utilize heterotic key problem in technology on bluish dogbane produces.
In September, 2008, " selection of bluish dogbane Yebai cytoplasmic male sterile line " of the Zhou Ruiyang of Guangxi University invention obtains national inventing patent (ZL200510019454.4, publication number CN100415083C).It is characterized in that take that bluish dogbane wild species UG93 or heredity have the cytoplasmic filial generation of bluish dogbane wild species UG93 as maternal, take bluish dogbane cultivar as male parent, carry out saturated backcrossing, therefrom select proterties similar to male parent, neat and consistent, and show male sterile Yebai cytoplasmic male sterile line, its male parent used that backcrosses is male sterility maintainer line.The male sterile line of seed selection and crossbreed thereof are UG93 cytoplasm thus.
On December 5th, 2012, " selection of bluish dogbane H040 cytoplasmic male sterile line " of the Zhou Ruiyang of Guangxi University invention obtains national inventing patent (ZL200810073873.X).It is characterized in that take that bluish dogbane wild species H040 or heredity have the cytoplasmic filial generation of bluish dogbane wild species H040 as maternal, take bluish dogbane cultivar as male parent, carry out saturated backcrossing, therefrom select proterties similar to male parent, neat and consistent, and show male sterile Yebai cytoplasmic male sterile line, its male parent used that backcrosses is male sterility maintainer line.The male sterile line of seed selection and crossbreed thereof are H040 cytoplasm thus.
This area similarly invention also has: Yuan Longping etc.s be take the natural abortion mutant found in wild rice as female parent, by the method for continuous backcross, select the cytoplasmic male sterile lines such as precious Xian 97A in 1973.1980, hybrid rice transferred the U.S. as first agrotechnique patent of China.But as far back as nineteen sixty-eight, No. 5 hybridization of Japanese agriculture technical research institute Du Bianyi Burma Reed rice (Lead rice) and rattan slope, pass through continuous backcross, be bred as and there are No. 5 male sterile lines of the cytoplasmic rattan slope of Reed and (seen: the chief editor of the Chinese Academy of Agricultural Sciences, < < China rice makes to learn the 331st page～334 page the 2nd row of > >, agricultural publishing house, 1986), early than Mr. Yuan Longping.
But in agricultural production, there is the pandemic risk of a certain disease in spread and long-term utilization by the crossbreed of single cell matter male sterile line assembly, because the crossbreed of this cytoplasmic male sterile line assembly may become the nurture kind of a certain pathogenic microorganism biological strain.The Basic Ways addressing this problem is the male sterile line that selects various kinds of cell matter source, and promotes aborning the three line hybrid seed in different cytoplasm source.
Summary of the invention
The object of the invention is to solve the problem in bluish dogbane crossbreed single cell matter source, a kind of selection of novel bluish dogbane cytoplasmic male sterile line is provided, utilize the method for the sterile cytoplasm seed selection male sterile line of bluish dogbane KN142.
KN142 was the bluish dogbane wild species of Kenya, by Hemp Inst., China Academy of Agricultural Sciences, was provided.Inventor finds that by Molecular Detection the cytoplasm of the bluish dogbane wild species KN142 that can educate is sterile cytoplasm, then plant the seed of the spontaneous pollination of gathering on this product plant so that hybridization transformation, the individual plant performance male sterile that after plantation wherein there is discovery, this seed selection that is male sterile line provides great convenience.
The present invention is with 1) bluish dogbane wild species KN142,2) the sterile strain that KN142 seminal propagation offspring obtains, or 3) to have the cytoplasmic hybridization of bluish dogbane wild species KN142 or backcross progeny be cytoplasm donor in heredity, take common bluish dogbane cultivar (being) as cell nucleus donor again, and consequent nucleo-cytoplasmic interaction causes male sterile.
The method that the present invention is to provide the sterile cytoplasm seed selection male sterile line that utilizes bluish dogbane KN142, concrete steps are as follows:
The breeding material that contains bluish dogbane KN142 sterile cytoplasm of take is female parent, and the bluish dogbane cultivar of take carries out saturated backcrossing as male parent, therefrom selects proterties similar to male parent, neat and consistent, and show male sterile Yebai cytoplasmic male sterile line; Its male parent used that backcrosses is corresponding male sterility maintainer line.
Wherein, the breeding material that contains bluish dogbane KN142 sterile cytoplasm described in is one of following 3 kinds of breeding materials:
1) bluish dogbane wild species KN142;
2) the sterile strain of KN142 seminal propagation offspring gained;
3) heredity has hybridization or the backcross progeny of KN142 sterile cytoplasm.
Wherein, when backcrossing described in carrying out, measure coordinate force with a plurality of cultivars, each is selected from generation to generation, and coordinate force is high, proterties is similar to male parent, and the indehiscent male sterile plant of flower pesticide continues to backcross, while male parent selfing.
Wherein, the algebraically backcrossing described in is more than 5 generations (containing).
Wherein, described coordinate force is high refers to that coordinate force is more than mean value, measures the concrete grammar of coordinate force with reference to < < plant breeding > >.
The application of the method that the present invention also provides the sterile cytoplasm seed selection male sterile line that utilizes bluish dogbane KN142 in bluish dogbane breeding.
The application of the male sterile line that method is educated that the present invention further provides the sterile cytoplasm seed selection male sterile line that utilizes bluish dogbane KN142 in bluish dogbane breeding.
Method of the present invention, has the following advantages:
Bluish dogbane wild species KN142 cytoplasmic male sterile line and other bluish dogbane cultivar natural crossing combining go out cross combination which has strong superior, and breeding cost is low.By the crossbreed of the type cytoplasmic male sterile line assembly and the crossbreed of UG93 or the assembly of H040 cytoplasmic male sterile line utilization and extention simultaneously aborning, can greatly reduce the risk of the large generation of certain disease.
Embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
Embodiment 1 is with bluish dogbane wild species KN142 transformation male sterile line
Take bluish dogbane wild species KN142 as maternal, rich No. 1 B(in Guangdong of take is shown in: be national inventing patent ZL200510019454.4 embodiment 3) male parent, adopt the saturated method backcrossing, selected male sterile line K4P3A and the maintainer K4P3B thereof with KN142 male sterile cytoplasm.
Specific as follows:
2008-2010, take KN142 as maternal, rich No. 1 B in Guangdong of take is paternal hybrid, rich No. 1 B in Guangdong of take afterwards backcrosses as male parent, and when backcrossing, the good fortune of take red 992 and R1 are paternal hybrid, measure coordinate force, each is selected from generation to generation, and coordinate force is high, proterties is similar to male parent, and the indehiscent male sterile plant of flower pesticide continues to backcross, simultaneously male parent selfing.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, obtain K4P3A, the Characters is similar to rich No. 1 B in Guangdong, and fertility shows as male sterile.Its maintainer is K4P3B.
Embodiment 2 is with the sterile strain transformation of KN142 seminal propagation offspring gained male sterile line
The seed of results bluish dogbane wild species KN142 spontaneous pollination, plantation obtains seminal propagation offspring, and therefrom screening obtains sterile strain.The sterile strain of the gained of take is as maternal, rich No. 1 B(in Guangdong of take is shown in: be national inventing patent ZL200510019454.4 embodiment 3) male parent, adopt the saturated method backcrossing, selected male sterile line K4TP3A and the maintainer K4TP3B thereof with KN142 male sterile cytoplasm.
Specific as follows:
2010-2012, the male sterile plant of finding in KN142 seminal propagation offspring of take is maternal, rich No. 1 B in Guangdong of take is paternal hybrid, rich No. 1 B in Guangdong of take afterwards backcrosses as male parent, and when backcrossing, the good fortune of take red 992 and R1 are paternal hybrid, measure coordinate force, each is selected from generation to generation, and coordinate force is high, proterties is similar to male parent, and the indehiscent male sterile plant of flower pesticide continues to backcross, simultaneously male parent selfing.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, obtain K4TP3A, the Characters is similar to rich No. 1 B in Guangdong, and fertility shows as male sterile.Its maintainer is K4TP3B.
Embodiment 3 is with bluish dogbane wild species KN142 transformation male sterile line
Take bluish dogbane wild species KN142 as maternal, and the bluish dogbane conventional cultivation kind golden light of take is stingless, and (University Of Agriculture and Forestry In Fujian's introduction) is male parent, adopts the saturated method backcrossing, and selects and has the cytoplasmic male sterile line K4K3A of KN142 male sterile line and maintainer K4K3B thereof.
Specific as follows:
2010-2012, take KN142 as maternal, the golden light of take is stingless is paternal hybrid, the golden light of take is afterwards stingless to be backcrossed as male parent, and when backcrossing, the good fortune of take red 992 and R1 are paternal hybrid, measure coordinate force, each is selected from generation to generation, and coordinate force is high, proterties is similar to male parent, and the indehiscent male sterile plant of flower pesticide continues to backcross, simultaneously male parent selfing.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, obtain K4K3A, the Characters is stingless similar to golden light, and fertility shows as male sterile.Its maintainer is K4K3B.
Embodiment 4 is with the sterile strain transformation of KN142 seminal propagation offspring gained male sterile line
The seed of results bluish dogbane wild species KN142 spontaneous pollination, plantation obtains seminal propagation offspring, and therefrom screening obtains sterile strain.The sterile strain of the gained of take is as maternal, and the bluish dogbane conventional cultivation kind golden light of take is stingless, and (University Of Agriculture and Forestry In Fujian's introduction) is male parent, adopts the saturated method backcrossing, and selects and has the cytoplasmic male sterile line K4TK3A of KN142 male sterile line and maintainer K4TK3B thereof.
Specific as follows:
2010-2012, the male sterile plant of finding in KN142 seminal propagation offspring of take is maternal, the golden light of take is stingless is paternal hybrid, the golden light of take is afterwards stingless to be backcrossed as male parent, and when backcrossing, the good fortune of take red 992 and R1 are paternal hybrid, measure coordinate force, each is selected from generation to generation, and coordinate force is high, proterties is similar to male parent, and the indehiscent male sterile plant of flower pesticide continues to backcross, simultaneously male parent selfing.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, obtain K4TK3A, the Characters is stingless similar to golden light, and fertility shows as male sterile.Its maintainer is K4TK3B.
Embodiment 5 has the filial generation transformation male sterile line of KN142 sterile cytoplasm with heredity
The first-filial generation of bluish dogbane wild species bluish dogbane wild species rich No. 1 B in KN142 * Guangdong of take is maternal, with bluish dogbane cultivated species good fortune, (have another name called F3A red No. 3, see patent of invention ZL200510019454.4, publication number CN100415083C, embodiment 4) be male parent, adopt the saturated method backcrossing, select male sterile line K4F3A and the maintainer K4F3B thereof with KN142 sterile cytoplasm.
Specific as follows:
2011-2013, the first-filial generation of rich No. 1 B in bluish dogbane wild species KN142 * Guangdong of take is maternal, the good fortune of take is red is for No. 3 paternal hybrid, the good fortune of take afterwards backcrosses as male parent for red No. 3, and when backcrossing, the good fortune of take red 992 and R1 are paternal hybrid, measure coordinate force, each is selected from generation to generation, and coordinate force is high, proterties is similar to male parent, and the indehiscent male sterile plant of flower pesticide continues to backcross, simultaneously male parent selfing.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, obtain K4F3A, the Characters and good fortune red No. 3 similar, fertility shows as male sterile.Its maintainer is K4F3B.
Embodiment 6 has the filial generation transformation male sterile line of KN142 sterile cytoplasm with heredity
The first-filial generation of bluish dogbane wild species KN142 seminal propagation offspring's gained rich No. 1 B in sterile strain * Guangdong of take is maternal, with bluish dogbane cultivated species good fortune, (have another name called F3A red No. 3, see patent of invention ZL200510019454.4, publication number CN100415083C, embodiment 4) be male parent, adopt the saturated method backcrossing, select male sterile line K4TF3A and the maintainer K4TF3B thereof with KN142 sterile cytoplasm.
Specific as follows:
2011-2013, the first-filial generation of bluish dogbane wild species KN142 seminal propagation offspring's gained rich No. 1 B in sterile strain * Guangdong of take is maternal, the good fortune of take is red is for No. 3 paternal hybrid, the good fortune of take afterwards backcrosses as male parent for red No. 3, and when backcrossing, the good fortune of take red 992 and R1 are paternal hybrid, measure coordinate force, each is selected from generation to generation, and coordinate force is high, proterties is similar to male parent, and the indehiscent male sterile plant of flower pesticide continues to backcross, simultaneously male parent selfing.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, obtain K4TF3A, the Characters and good fortune red No. 3 similar, fertility shows as male sterile.Its maintainer is K4TF3B.
Embodiment 7 is with the sterile cytoplasm transformation male sterile line of the saturated gained K4P3A that backcrosses of embodiment 1
The embodiment 1 of take is saturated backcrosses gained K4P3A as maternal, take T17(Hemp Inst., China Academy of Agricultural Sciences to introduce) be male parent, adopt the saturated method backcrossing, selected male sterile line K4T17A and the maintainer K4T17B thereof with KN142 male sterile cytoplasm.
Specific as follows:
2011-2013, the saturated gained K4P3A that backcrosses of the embodiment 1 of take is as maternal, take T17(Hemp Inst., China Academy of Agricultural Sciences introduces) be male parent, the T17 of take afterwards backcrosses as male parent, and when backcrossing, the good fortune of take red 992 and R1 are paternal hybrid, measure coordinate force, each is selected from generation to generation, and coordinate force is high, proterties is similar to male parent, and the indehiscent male sterile plant of flower pesticide continues to backcross, simultaneously male parent selfing.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, obtain K4T17A, the Characters is similar to T17, and fertility shows as male sterile.Its maintainer is K4T17B.
Embodiment 8 has the filial generation transformation male sterile line of KN142 sterile cytoplasm with heredity
The first-filial generation that the bluish dogbane wild species KN142 * golden light of take is stingless is as maternal, with bluish dogbane cultivated species, 917(has another name called 917B, the Kenaf Cultivars of Hemp Inst., China Academy of Agricultural Sciences's seed selection) be male parent, adopt the saturated method backcrossing, selected male sterile line K4917A and the maintainer K4917B thereof with KN142 male sterile cytoplasm.
Specific as follows:
2011-2013, the first-filial generation that the bluish dogbane wild species KN142 * golden light of take is stingless is as maternal, take 917 as paternal hybrid, take afterwards and 917 backcross as male parent, when backcrossing, the good fortune of take red 992 and R1 are paternal hybrid, measure coordinate force, each is selected from generation to generation, and coordinate force is high, proterties is similar to male parent, and the indehiscent male sterile plant of flower pesticide continues to backcross, simultaneously male parent selfing.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, obtain K4917A, the Characters is similar to 917, and fertility shows as male sterile.Its maintainer is K4917B.
Embodiment 9 has the filial generation transformation male sterile line of KN142 sterile cytoplasm with heredity
The first-filial generation that the sterile strain * golden light of bluish dogbane wild species KN142 seminal propagation offspring's gained of take is stingless is as maternal, with bluish dogbane cultivated species, 917(has another name called 917B, the Kenaf Cultivars of Hemp Inst., China Academy of Agricultural Sciences's seed selection) be male parent, adopt the saturated method backcrossing, selected the male sterile line K4T917A with KN142 male sterile cytoplasm, and maintainer K4T917B.
Specific as follows:
2011-2013, the first-filial generation that the sterile strain * golden light of bluish dogbane wild species KN142 seminal propagation offspring's gained of take is stingless is as maternal, take 917 as paternal hybrid, take afterwards and 917 backcross as male parent, when backcrossing, the good fortune of take red 992 and R1 are paternal hybrid, measure coordinate force, each is selected from generation to generation, and coordinate force is high, proterties is similar to male parent, and the indehiscent male sterile plant of flower pesticide continues to backcross, simultaneously male parent selfing.In Nanning and Lingshui, Hainan, backcross every year, 6 generations that backcrossed altogether for 3 years, obtain K4T917A, the Characters is similar to 917, and fertility shows as male sterile.Its maintainer is K4T917B.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (6)
1. the method for utilizing the sterile cytoplasm seed selection male sterile line of bluish dogbane KN142, concrete steps are as follows:
The breeding material that contains bluish dogbane KN142 sterile cytoplasm of take is female parent, and the bluish dogbane cultivar of take carries out saturated backcrossing as male parent, therefrom selects proterties similar to male parent, neat and consistent, and show male sterile Yebai cytoplasmic male sterile line; Its male parent used that backcrosses is corresponding male sterility maintainer line.
2. method according to claim 1, is characterized in that, described in contain bluish dogbane KN142 sterile cytoplasm breeding material be one of following 3 kinds of breeding materials:
1) bluish dogbane wild species KN142;
2) the sterile strain of KN142 seminal propagation offspring gained;
3) heredity has hybridization or the backcross progeny of KN142 sterile cytoplasm.
3. according to the method described in claim 1-2 any one, it is characterized in that, when backcrossing described in carrying out, measure coordinate force with a plurality of cultivars, each is selected from generation to generation, and coordinate force is high, proterties is similar to male parent, and the indehiscent male sterile plant of flower pesticide continues to backcross, simultaneously male parent selfing.
4. according to the method described in claim 1-2 any one, it is characterized in that, described in the algebraically that backcrosses be more than 5 generations (containing).
5. the application of the method for sterile cytoplasm seed selection male sterile line of utilizing bluish dogbane KN142 described in claim 1-4 any one in bluish dogbane breeding.
6. the application of the male sterile line obtaining according to the method seed selection that utilizes the sterile cytoplasm seed selection male sterile line of bluish dogbane KN142 described in claim 1-4 any one in bluish dogbane breeding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310711047.4A CN103733976B (en) | 2013-12-19 | 2013-12-19 | Utilize the method for the sterile cytoplasm seed selection male sterile line of bluish dogbane KN142 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310711047.4A CN103733976B (en) | 2013-12-19 | 2013-12-19 | Utilize the method for the sterile cytoplasm seed selection male sterile line of bluish dogbane KN142 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103733976A true CN103733976A (en) | 2014-04-23 |
| CN103733976B CN103733976B (en) | 2015-09-16 |
Family
ID=50491116
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310711047.4A Active CN103733976B (en) | 2013-12-19 | 2013-12-19 | Utilize the method for the sterile cytoplasm seed selection male sterile line of bluish dogbane KN142 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103733976B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104620978A (en) * | 2015-02-10 | 2015-05-20 | 福建农林大学 | Breeding method of cytoplasmic male sterile line of quality disease-resistant photo periodical insensitive hibiscus cannabinus |
| CN104798679A (en) * | 2015-05-12 | 2015-07-29 | 中国农业科学院麻类研究所 | Breeding method of light insensitive kenaf male sterility line 261N5-18A |
| CN106636194A (en) * | 2017-01-25 | 2017-05-10 | 广西大学 | Method for creating cytoplasmic male sterile line through transgenosis |
| CN107996389A (en) * | 2017-12-04 | 2018-05-08 | 广西大学 | A kind of selection of bluish dogbane cultigen cytoplasmic male sterile line |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1748469A (en) * | 2005-09-17 | 2006-03-22 | 广西大学 | Breeding method for bluish dogbane Yebai cytoplasmic male sterile line |
| CN101401540A (en) * | 2008-11-03 | 2009-04-08 | 广西大学 | Hibiscus cannabinus l. H040 cytoplasmic male sterility phyletic breeding method |
-
2013
- 2013-12-19 CN CN201310711047.4A patent/CN103733976B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1748469A (en) * | 2005-09-17 | 2006-03-22 | 广西大学 | Breeding method for bluish dogbane Yebai cytoplasmic male sterile line |
| CN101401540A (en) * | 2008-11-03 | 2009-04-08 | 广西大学 | Hibiscus cannabinus l. H040 cytoplasmic male sterility phyletic breeding method |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104620978A (en) * | 2015-02-10 | 2015-05-20 | 福建农林大学 | Breeding method of cytoplasmic male sterile line of quality disease-resistant photo periodical insensitive hibiscus cannabinus |
| CN104798679A (en) * | 2015-05-12 | 2015-07-29 | 中国农业科学院麻类研究所 | Breeding method of light insensitive kenaf male sterility line 261N5-18A |
| CN104798679B (en) * | 2015-05-12 | 2017-04-12 | 中国农业科学院麻类研究所 | Breeding method of light insensitive kenaf male sterility line 261N5-18A |
| CN106636194A (en) * | 2017-01-25 | 2017-05-10 | 广西大学 | Method for creating cytoplasmic male sterile line through transgenosis |
| CN106636194B (en) * | 2017-01-25 | 2019-10-29 | 广西大学 | The method of Transgenic Rice initiative cytoplasmic male sterile line |
| CN107996389A (en) * | 2017-12-04 | 2018-05-08 | 广西大学 | A kind of selection of bluish dogbane cultigen cytoplasmic male sterile line |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103733976B (en) | 2015-09-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103125368B (en) | Breeding method of insect-resistant big-stigma three-line sterile line | |
| CN104137764A (en) | Breeding method of three-line genic male sterile line of high-resistance glabrous yellow-leaf giant embryo rice | |
| CN103733976B (en) | Utilize the method for the sterile cytoplasm seed selection male sterile line of bluish dogbane KN142 | |
| CN103733978B (en) | Utilize the method for the sterile cytoplasm seed selection male sterile line of bluish dogbane KN250 | |
| CN104719129A (en) | Breeding method of novel wheat variety | |
| CN103814814A (en) | Method for selective breeding of rice thermo-sensitive permanent genic male sterile line by adopting bridge parents | |
| CN106665332A (en) | Method for carrying out recurrent selection breeding on water-saving and drought-resisting rice by utilizing dominant nuclear male sterile material | |
| CN101401540B (en) | Hibiscus cannabinus l. H040 cytoplasmic male sterility phyletic breeding method | |
| CN100415083C (en) | Breeding method for bluish dogbane Yebai cytoplasmic male sterile line | |
| CN104604736A (en) | Sesame photoperiod sensitive genic male sterile line and hybrid seed production method thereof | |
| Saito et al. | A novel source of cytoplasmic male sterility and a fertility restoration gene in eggplant (Solanum melongena L.) lines | |
| CN103749280B (en) | Utilize the method for the sterile cytoplasm seed selection male sterile line of bluish dogbane ZB90 | |
| CN103444515A (en) | Transfer method for male sterile line of purple wuta-tsai | |
| CN104642092A (en) | Selection method for onion male sterility maintainer line | |
| Reddy et al. | Cytoplasmic genetic male sterility in chilli (Capsicum annuum L.) | |
| CN102550394A (en) | Cold-region japonica hybrid rice restorer and variety breeding method | |
| CN106035069A (en) | Breeding method for hybrid rice two-line sterility line with albino ear lemmas | |
| CN1486596A (en) | Cross breeding process of color trilinear cotton hybrid | |
| CN104542258A (en) | Method for constructing polyploidy rice PMeSnear-isogenic line by using generation-skipping backcross | |
| CN105613257A (en) | Recurrent selection method for cotton sterile line | |
| CN104813927A (en) | Selective breeding method of anti-wilt eggplant cytoplasmic male sterility restoring line | |
| CN107372096A (en) | A kind of method of quick breeding nucleo-cytoplasmic interreaction sterile line | |
| Pitchaimuthu et al. | Studies on inheritance of Geneic Male Sterility (GMS) and hybrid seed production in okra [Abelmoschus esculentus (L.) Moench.] | |
| Rubis | Developing new characteristics during 50 years of safflower breeding. | |
| CN105961187A (en) | Breeding method of main inflorescence polyhedron brassica napus cytoplasm male sterility restorer line |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |