CN102487811B - Breeding method and application of middle indica-tendency type rice semi-dwarf photosensitive sterile line - Google Patents
Breeding method and application of middle indica-tendency type rice semi-dwarf photosensitive sterile line Download PDFInfo
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- CN102487811B CN102487811B CN201110403905.XA CN201110403905A CN102487811B CN 102487811 B CN102487811 B CN 102487811B CN 201110403905 A CN201110403905 A CN 201110403905A CN 102487811 B CN102487811 B CN 102487811B
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Abstract
The invention discloses breeding method and application of a middle indica-tendency type rice semi-dwarf photosensitive sterile line Y98149IS. The breeding method comprises the following steps of: artificially hybridizing the japonica type rice semi-dwarf photosensitive sterile line Y98149S carrying a rice dominant semi-dwarf mutant gene and a photosensitive nuclear male sterile gene as a female parent with an indica type rice advanced line 01E1-188 carrying a rice dominant semi-dwarf mutant gene, strictly choosing male sterility and indica and japonica parting characters of plant individuals in segregation populations in genetic segregation generations to successively breed the middle indica-tendency type rice semi-dwarf photosensitive sterile line Y98149IS. The invention also discloses an application of preparing a hybrid rice combination to rice production by using the bred Y98149IS as a female parent and the rice male sterile restoring line as a male parent. The bred Y98149IS can be applied to the breeding of a hybrid rice variety, the plant height of the bred hybrid rice variety can be effectively reduced, the plant morphology is improved, and the lodging resistance, the yield stability and the like of the bred hybrid rice combination are further increased.
Description
Technical field
The invention belongs to rice heterosis utilization technical field, relate to the selection of rice strain, is more particularly selection and the application of a kind of middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line Y98149IS.
Background technology
Paddy rice is many countries, the especially important cereal crops of the state such as China, India, Japan in the world.China is the first populous nation in the world, and be also one of main in the world eating rice production state, Rice Production occupies very important status in the grain-production of China.The rice breeding of China, via the improved variety of short stem of the sixties in 20th century and the hybridization improved variety of the seventies, successfully make use of the recessive Semi dwarfism gene coming from China's rice variety low pin crow point
sd-1and rice heterosis, improve the yield level of China and world paddy rice significantly.But along with deepening continuously of kind improved variety process, the genetic diversity of improving the breed presents downward trend.
It is reported, the genetic distance in the genetic distance in Asian Cultivated Rice kind and subspecies between type is about the same, and genetic distance between China's paddy rice improved variety at present and parent thereof is not as good as the half of subspecies genetic distance; The improved variety of south China long-grained nonglutinous rice 75% has
sd-1short source blood lineage.Hybrids of Indica and Japonica significantly can widen the hereditary basis of hybrid, improves F
1the biological yield in generation, but indica and japonica subspecies hybrid F
1for the super close bottleneck constituting again inter-subspecific heterosis and utilize of plant height.Because of by Genetic Diversity of Germplasm, since eighties of last century eighties, never there is large breakthrough in the yield level of China and even world's rice breeding.
Resource of short stem is the basis that the excellent plant type of rice varieties is achieved.The qualitative character heredity that the heredity of paddy rice nanism has key-gene to control and quantitative inheritance two kinds of modes of controlled by multiple genes.The dwarf gene in paddy rice mainly Recessive genes of application in production.That has reported has
sd-1,
sd-gwith
sd-s (t)deng, wherein
sd-1be called as green revolution gene.About the report of dominant dwarf rice gene is few.The heavy hero of China fir, rock Tian Shenfu obtain the short raw mutant of dominant many tillers by No. 8, gamma-rays process agricultural.Yellow Cathay, Zhou Kaida report that the short strain DI in Yunnan half is dominant dwarf, but its dominance action is indefinite.Zhu Lihong etc. show the research that the short source KL908 of Japan carries out, and its nanism controls by a pair weak part dominant gene, and gene expression affects larger by genetic background.
The unification that dwarf gene utilizes and recessivation, result in paddy rice Cultivars genetic background convergent, become the key factor hindering China's Yield of Rice Varieties potentiality to improve further.Discovering and using of new dwarf gene, especially indication law, solves the recessivation of dwarf gene utilization and the problem of unification by contributing to, significant to the hereditary basis of widening paddy rice Cultivars.
Because dominant dwarf resource directly can also be used in breeding of hybridized rice research, in being utilized with indica-japonica heterosis if apply, the super close problem of plant height of indica and japonica subspecies hybrid generation also effectively can be solved.Thus it has and is more importantly worth in rice high yield Breeding For Super High-yielding.
Summary of the invention
The object of the invention is to the round-grained rice type photosensitive sterile line Y98149S carrying paddy rice dominant semi Dwarf variants gene and photosensitive Male sterile gene of institute's seed selection, artificial hybridization is carried out with the Indica Rice advanced generation line 01E1-188 carrying paddy rice dominant semi Dwarf variants gene, Genetic Recombination, and then the middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line that seed selection is new, thering is provided the use of hybrid rice especially Intersubspecific Hybrid Rice breed breeding, is the resources approach that the super close problem exploration of plant height solved in rice high yield and SOYBEAN IN HIGH-YIELD BREEDING process is new.
The present invention is achieved by the following technical solutions.
The selection of a kind of middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line Y98149IS, it is characterized in that utilizing the round-grained rice type photosensitive sterile line Y98149S carrying paddy rice dominant semi Dwarf variants gene and photosensitive Male sterile gene to be female parent, artificial hybridization is carried out with the Indica Rice advanced generation line 01E1-188 carrying paddy rice dominant semi Dwarf variants gene, at hereditary segregative generation, the male sterility of individual plants in segregation population and Xian round-grained rice somatotype proterties are strictly selected, selected by educate successful middle indica-tendency type semi-dwarf mutant photosensitive sterile line Y98149IS.
Paddy rice semi-dwarf mutant round-grained rice type of the present invention photosensitive genic male sterile line Y98149S refers to the photosensitive genic male sterile line of semi-dwarf mutant round-grained rice type (selection cross method refers to " Agriculture of Anhui science " 2005,33 (10): 1789-1790) of paddy rice Semi-dwarf mutant Y98149 and round-grained rice type photosensitive genic male sterile line 4008S selection cross.
Paddy rice semi-dwarf mutant Indica Rice of the present invention pure lines material 01E1-188 refers to that paddy rice Semi-dwarf mutant Y98149 examines rice No. 200002 with the magnificent round-grained rice Xian 74(Guangdong of indica rice variety, Jiangxi examines rice 2002013) the Indica Rice semi-dwarf mutant strain of selection cross.
The present inventor in 1997 at middle round-grained rice hybrid combination (M9056/R8018 choosing) F
6in strain, find a Semi-dwarf mutant (referring to " rice in China science " 2001,15 (4): 314-316).This Semi-dwarf mutant plant height 73cm, be only 74.1% of wild type plant height, the result of study of having carried out shows, the nanism of this Semi-dwarf mutant controls by a pair dominant nuclear gene, the non-equipotential of Semi dwarfism gene heredity with reporting, belongs to new Semi dwarfism gene resource (referring to " Acta Agronomica Sinica " 2003. (3): 519-524).
The present invention further discloses the application of middle indica-tendency type semi-dwarf mutant photosensitive sterile line Y98149IS in breeding rice high yield super high yield crops.
The application method of middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line Y98149IS of the present invention, for female parent with the semi-dwarf mutant middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line Y98149IS of institute's seed selection, recover to be male parent with male sterility of rice, preparing hybrid Rice Combination and be applied to Rice Production, the paddy rice produced effectively reduces the plant height of hybrid rice, therefore, the lodging resistance of hybrid rice can effectively be improve.
The good effect that the selection of middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line Y98149IS disclosed by the invention is compared with prior art had is:
(1) the present invention utilizes the round-grained rice type photosensitive sterile line Y98149S carrying paddy rice dominant semi Dwarf variants gene and Photosensitive genie male sterile gene to be female parent, artificial hybridization is carried out with the Indica Rice advanced generation line 01E1-188 carrying paddy rice dominant semi Dwarf variants gene, carry out systematic breeding, and the middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line that seed selection is new, to provide the use of hybrid rice especially Intersubspecific Hybrid Rice breed breeding, it is the resources approach that the super close problem exploration of plant height solved in rice varieties and subspecies high yield and Breeding For Super High-yielding process is new.
(2) the lodging sex chromosome mosaicism of rice varieties is own through becoming the important limiting factor of Rice Production and rice variety selective.Lodging not only causes Yield of Rice Varieties to decline, but also causes the change of rice quality bad.Affecting among the relevant factors of the anti-fall property of rice varieties, plant height is one of most important factor.The middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line Y98149IS of seed selection of the present invention effectively can reduce the plant height of the hybrid rice of institute's seed selection, improves the lodging resistance etc. of the hybrid rice of institute's seed selection.
Accompanying drawing explanation
Fig. 1 is Y98149IS selection flow chart of the present invention;
Fig. 2 is Y98149IS preparing hybrid Rice Combination flow chart of the present invention;
Fig. 3 is Y98149IS preparing hybrid paddy rice practical application flow chart of the present invention.
Embodiment
Below in conjunction with embodiment, the present invention is described, the scheme of embodiment described here, do not limit the present invention, one of skill in the art can make improvements and change according to spirit of the present invention, these described improvement and change all should be considered as within the scope of the invention, and scope of the present invention and essence are limited by claim.Its Chinese round-grained rice Xian 74 is authorization recommended variety (Guangdong examine rice No. 200002, Jiangxi examine rice 2002013).
Embodiment 1
See Fig. 1, the selection of middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line Y98149IS, mainly through building with under type:
In Hefei paddy rice normal growth season, paddy rice dominant semi selected by utilization photosensitive sterile line Y98149S of short stem is female parent, to carry the Indica Rice advanced generation line 01E1-188 of paddy rice dominant semi Dwarf variants gene for male parent, carry out artificial hybridization, obtain artificial hybridization F
1seed.
In numerous season in Hainan winter, plantation hybrid F
1plant, rejects false hybrid, breeding F
2seed.
In the paddy rice normal growth season in Hefei, plantation F
2plant.The male-sterile character of plant and Xian round-grained rice somatotype proterties are strictly selected simultaneously.Select plant to downgrade male completely sterile indica plant and cut stubble, breeding F
3seed.
Divide individual plant results F
3seed, takes and becomes F toward Hainan kind
3plant, selects the Xian round-grained rice somatotype proterties of plant and plant height, breeding F
4seed.
In the paddy rice normal growth season in Hefei, plantation F
4plant, strictly selects the male-sterile character of plant and Xian round-grained rice somatotype proterties simultaneously simultaneously.Selection plant downgrades and male completely sterile inclined Xian plant cuts stubble, breeding F
5seed.
Thereafter the F in numerous season in Hainan winter
5, F
7, F
9, F
11the artificial selection scheme of plant generation and hybrid F
1plant is from generation to generation identical, and the F in Hefei normal growth season
4, F
6, F
8, F
10the artificial selection scheme of plant generation and hybrid F
2plant is identical from generation to generation, till the Xian round-grained rice somatotype proterties and kernel male sterile proterties complete stability of institute's seed selection male sterile line.
Embodiment 2
The Breeding Application of the middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line Y98149IS of seed selection of the present invention
Be with the middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line Y98149IS of institute's seed selection for female parent see Fig. 2, recover to be male parent with male sterility of rice, preparing hybrid Rice Combination and be applied to Rice Production.The plant height of the hybrid rice prepared effectively reduces, and lodging resistance improves.
Embodiment 3
Middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line Y98149IS, in hybrid rice combo process, reduce join actual utility in cross hybrid F1 plant height.
For maternal (plant height 66.2cm) with the middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line Y98149IS of institute's seed selection see Fig. 3, with Japonica rice male sterile restoring line C418(plant height 112.6cm) for male parent, the plant height only 91.4cm of institute's preparing hybrid Rice Combination [(Y98149IS × C418)/F1)], plant height reduces amplitude and reaches 18.8%.
Claims (5)
1. the selection of a middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line Y98149IS, it is characterized in that utilizing the round-grained rice type photosensitive sterile line Y98149S carrying paddy rice dominant semi Dwarf variants gene and photosensitive Male sterile gene to be female parent, artificial hybridization is carried out with the Indica Rice advanced generation line 01E1-188 carrying paddy rice dominant semi Dwarf variants gene, at hereditary segregative generation, strictly select the male sterility of individual plants in segregation population and Xian round-grained rice somatotype proterties, seed selection obtains middle indica-tendency type semi-dwarf mutant photosensitive sterile line Y98149IS.
2. the selection of middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line Y98149IS described in claim 1, wherein maternal Y98149S refers to the photosensitive genic male sterile line of semi-dwarf mutant round-grained rice type of paddy rice Dominant semi-dwarf mutant Y98149 and round-grained rice type photosensitive genic male sterile line 4008S selection cross; Paddy rice semi-dwarf mutant Indica Rice pure lines material 01E1-188 refers to the Indica Rice semi-dwarf mutant strain of paddy rice Dominant semi-dwarf mutant Y98149 and indica rice variety China round-grained rice Xian 74 selection cross.
3. the application method of middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line Y98149IS described in claim 1, it is characterized in that: with the semi-dwarf mutant middle indica-tendency type rice semi-dwarf mutant photosensitive sterile line Y98149IS of institute's seed selection for female parent, recover to be male parent with male sterility of rice, preparing hybrid Rice Combination and be applied to Rice Production.
4. adopt the application of middle indica-tendency type semi-dwarf mutant photosensitive sterile line Y98149IS in breeding rice high yield, super high yield crops that claim 1 selection obtains.
5. the middle indica-tendency type semi-dwarf mutant photosensitive sterile line Y98149IS adopting claim 1 selection to obtain reduces the plant height of hybrid rice in preparation, improve the application of hybrid rice lodging resistance kind aspect.
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| CN201110403905.XA CN102487811B (en) | 2011-12-08 | 2011-12-08 | Breeding method and application of middle indica-tendency type rice semi-dwarf photosensitive sterile line |
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| CN102487811B true CN102487811B (en) | 2014-12-31 |
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| CN103598085B (en) * | 2013-10-28 | 2015-09-23 | 江苏沿海地区农业科学研究所 | A kind of selection of photo-thermo sensitive male nuclear sterile rice line |
| CN112438197B (en) * | 2019-08-27 | 2022-03-11 | 湖南杂交水稻研究中心 | Breeding method of three-way cross hybrid of rice |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1868261A (en) * | 2006-06-10 | 2006-11-29 | 安徽省农业科学院水稻研究所 | Method for breeding male sterible series of rice, and its application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1868261A (en) * | 2006-06-10 | 2006-11-29 | 安徽省农业科学院水稻研究所 | Method for breeding male sterible series of rice, and its application |
Non-Patent Citations (3)
| Title |
|---|
| "水稻显性半矮杆基因对株高表达的影响及其对GA3的敏感性";程灿等;《中国水稻科学》;20061231;第20卷(第1期);第25-30页 * |
| "水稻显性半矮秆若干遗传特性的研究";程灿;《中国优秀硕士学位论文全文数据库 农业科技辑》;20050915(第5期);第D047-19页 * |
| 程灿等."携带显性半矮秆基因粳型光(温)敏核不育系的配合力分析".《安徽农业科学》.2005,第33卷(第10期),第1789-1790页. * |
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