CN109349102A - A kind of long grain japonica rice two-line sterile line selection - Google Patents
A kind of long grain japonica rice two-line sterile line selection Download PDFInfo
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- CN109349102A CN109349102A CN201811511411.1A CN201811511411A CN109349102A CN 109349102 A CN109349102 A CN 109349102A CN 201811511411 A CN201811511411 A CN 201811511411A CN 109349102 A CN109349102 A CN 109349102A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
A kind of long grain japonica rice two-line sterile line selection, belongs to hybrid rice Biotechnology in Genetic Breeding field.It is specifically included: being selected long grain Restoring Line of Japonica Rice and is kept based material；Select the indica type two-line sterile line with excellent economical character；Above-mentioned rice material is hybridized, be returned, is reestablished diplomatic relations 1-2 times；Offspring is separated in several backcrossings, two system's infertility single plant of rice grain length >=6.5mm or more is selected to harvest seed；Long grain japonica rice infertility single plant is planted, is continuously selfed or continues backcrossing 1-3 times, obtain stable long grain japonica rice two-line sterile line material；Light temperature culture identification, Xian round-grained rice assessment of indices, economical character selection are carried out to long grain japonica rice two-line sterile line material, to obtain long grain japonica rice two-line sterile line.Long grain japonica rice two-line sterile line of the invention, plant type is ideal, and best in quality, Other Main Agronomic Characters are preferable, and flowering habit is good, and stigma exposing ratio and outcrossing seed-setting rate are high, blast resisting or other pest and disease damages.
Description
Technical field
The invention belongs to hybrid rice Biotechnology in Genetic Breeding fields, and in particular to a kind of long grain japonica rice two-line sterile line breeding
Method.
Background technique
China found fertility in the big Tanaka of Hubei overflowing sun (existing Xiantao City) Shahu Lake seed farm evening japonica rice land reclamation and cultivation 58 in 1973
A kind of special rice pest insects Nongken 58S of the environmental influences such as light, temperature, is put forward for the first time long day high temperature for 1981 in the world
Breeding under the lower production of hybrid seeds, short day low temperature, one is the imagination (Shi Mingsong, 1981) of dual-purpose breeding hybrid paddy rice, is attracted extensive attention.1988
Year Hunan agricultural school An Jiang finds and is bred as the long-grained nonglutinous rice two-line sterile line of separate sources to pacify agriculture S-1(Deng Hua phoenix etc. again, and 1999).
It additionally independently has found a plant 1S, 8608S, HD9802S, congratulate (Si Huamin etc., 2012) such as S, weighing apparatus agriculture S, land 18S.For decades,
Using Nongken 58S and An Nong S-1 etc. as main parent, rice in China breeder has cultivated 471 two-line sterile lines and has passed through
Technical appraisement above the provincial level, intermediate keng rice two-line sterile line 39 (national rice data center, http: //
Www.ricedata.cn/), parent basis has been established for the developing development of China's double-linear hybrid rice.
It is similar with traditional japonica rice (also to be limited by traditional understanding, it is believed that the long short grain of grain japonica rice of long-grained nonglutinous rice；Cheng Kansheng, 1984), I
The japonica rice two-line sterile line that state is bred as so far, such as 7001S, N5088S are short grain granule (rice grain length 5.0-
5.5mm, length-width ratio is 2.0 or so), correspondingly, the two line japonica hybrid rice applied in production is also short grain granule (rice grain length
5.0-5.5mm), sterile line flowering habit is poor, and the production of hybrid seeds is difficult, limits the development of Japonica Hybrid to a certain extent.
The applicant has invented a kind of long grain Japonica Hybrid selection (ZL 201210152095.X), Yi Zhongchang before
Grain hybrid japonica rice restorer selection method (ZL201210152908.5), has invented long grain Japonica Hybrid.And in breeding in 2012
At first, China without awn length grain japonica rice sterile, start to be applied to production (Shen Xihong etc., 2014).On this basis, it sends out
A kind of new type material is cultivated in a kind of bright long grain japonica rice two-line sterile line breeding method, proposition --- long two system of grain japonica rice infertility
System, significantly changes grain shape, flowering habit, stigma exposing ratio, to significantly improve outcrossing seed-setting rate, improve hybrid seed yield, rice product
Matter etc., and then long grain shape two line japonica hybrid rice is greatly developed, the appearance and market quality of existing two line japonica hybrid rice are significantly improved,
Adapt to the japonica rice market demand being growing at present.
Summary of the invention
In view of the problems of the existing technology, it is an object of the invention to design to provide a kind of long grain japonica rice two-line sterile line
The technical solution of selection.The long grain japonica rice two-line sterile line that breeding of the present invention obtains can be used in cultivating and promoting long grain shape
Two line japonica hybrid rice can improve the appearance and market quality of existing two line japonica hybrid rice, adapt to the country being growing at present
The outer market demand and urban and rural residents' eating requirements, it can also be used to develop long grain shape two line japonica hybrid rice seed industry.
A kind of long grain japonica rice two-line sterile line selection, it is characterised in that the following steps are included:
1) the long grain japonica rice that selection patent No. ZL 201210152095.X and patent No. ZL 201210152908.5 is related to restores
System and holding based material, as receptor or donor parents；
2) select the indica type two-line sterile line with excellent economical character as donor or receptor parent, the indica type two systems infertility
The sterile gene of system comes from Nongken 58S and An Nong S；
3) step 1) is hybridized with the rice material of step 2, is then returned using step 1) as male parent or female parent
It hands over, reestablish diplomatic relations 1-2 times, making long grain japonica rice gene and two is that sterile gene or other beneficial genes recombinate；
4) offspring is separated in several backcrossings that step 3) obtains, and selects two system's infertility single plant of rice grain length >=6.5mm or more
Harvest seed；
5) plantation step 4) the long grain japonica rice infertility single plant that obtains, continuous selfing or backcrossing 1-3 times obtain stable long grain japonica rice
Two-line sterile line material；
6) light temperature culture identification, Xian round-grained rice assessment of indices, round-grained rice are carried out to the long grain japonica rice two-line sterile line material that step 5) obtains
Index > 0.75, economical character selection, to obtain long grain japonica rice two-line sterile line.
A kind of long grain japonica rice two-line sterile line selection, it is characterised in that long grain japonica rice in the step 1)
Restorer be L917, L42 or their breeding Derivative line and intermediate materials, holdings based material for long round-grained rice 1B, long round-grained rice 2B or they
Breeding Derivative line and intermediate materials.
A kind of long grain japonica rice two-line sterile line selection, it is characterised in that indica type two system in the step 2)
Sterile line is Peiai 64S and Y58S.
A kind of long grain japonica rice two-line sterile line selection, it is characterised in that the step 3) and 4) middle separation
Offspring includes the F2-F5 segregating generation once hybridized, or the F2-F5 separation generation reestablished diplomatic relations and be returned again after primary hybridization
Generation.
A kind of long grain japonica rice two-line sterile line selection, it is characterised in that the step 4) and 5) middle acquisition
It is that selection has long kernel Characters in once hybridization segregating population that rice grain length >=6.5mm selection, which is two kinds: one,
Rice single plant, it is desirable that select, then the excellent single plant of reselection economical character, obtain in rice grain length >=6.5mm or more single plant
Long grain japonica rice two is sterile material；Two be that the long grain japonica rice material for obtaining primary election is further returned, make other beneficial genes with
Long kernel Characters polymerization, obtaining long grain japonica rice two is sterile material.
A kind of long grain japonica rice two-line sterile line selection, it is characterised in that light temperature culture in the step 6)
Identification is that manually climate box identifies that long grain japonica rice two is the Fertility Transfer Temperature of sterile material, and Xian round-grained rice index or round-grained rice sex index are surveyed
It surely is that the Indica or japonica for being bred as material is measured with the molecular labeling of difference Xian round-grained rice difference, it is raw that the selection of economical character meets crop field
It produces and requires.
A kind of long grain japonica rice two-line sterile line selection, it is characterised in that the length completed in the step 6)
It is sterile material that grain japonica rice two-line sterile line, which is the novel long grain japonica rice two of one kind, and Fertility al-teration is stablized, round-grained rice sex index >
0.75, it can apply for provincial technical appraisement, prepare the long grain Japonica Hybrid of two systems, be developed for Hybrid breeding in commercial system.
A kind of long grain japonica rice two-line sterile line selection, it is characterised in that the length completed in the step 6)
Rice grain length >=6.5mm of grain japonica rice two-line sterile line.
It is of the invention to be advantageous in that: to be suitable for carrying out grain shape improvement to existing japonica rice two-line sterile line, change traditional rice
The round shaped grain type japonica rice two-line sterile line that grain length is 5.0-5.5mm is the long grain japonica rice two-line sterile line of rice grain length >=6.5mm, into
And combo cultivates the long grain Japonica Hybrid Combination nova (kind) of two systems of rice grain length >=6.5mm, significantly improves two line japonica hybrid rice
Rice appearance and quality.
Long grain japonica rice two-line sterile line of the invention, plant type is ideal, and best in quality, Other Main Agronomic Characters are preferable, habit of blooming
Property it is good, stigma exposing ratio and outcrossing seed-setting rate are high, blast resisting or other pest and disease damages.Breeding time is suitable, and can prepare is suitable for not
With the long grain Japonica Hybrid Combination nova of two systems of area plantation.
Specific embodiment
Further illustrate the present invention with reference to embodiments.
A kind of embodiment: long grain japonica rice two-line sterile line selection
1) the long grain japonica rice that selection patent No. ZL 201210152095.X and patent No. ZL 201210152908.5 is related to restores
System and holding based material, preferably long grain Restoring Line of Japonica Rice are L917, L42 or their breeding Derivative line and intermediate materials, are protected
Holding based material is long round-grained rice 1B, long round-grained rice 2B or their breeding Derivative line and intermediate materials, as receptor or donor parents；
2) select the indica type two-line sterile line with excellent economical character as donor or receptor parent, the indica type two systems infertility
The sterile gene of system comes from Nongken 58S and An Nong S, preferably Peiai 64S and Y58S；
3) step 1) is hybridized with the rice material of step 2, is then returned using step 1) as male parent or female parent
It hands over, reestablish diplomatic relations 1-2 times, making long grain japonica rice gene and two is that sterile gene or other beneficial genes recombinate；
4) offspring is separated in several backcrossings that step 3) obtains, and selects two system's infertility single plant of rice grain length >=6.5mm or more
Harvest seed；
5) plantation step 4) the long grain japonica rice infertility single plant that obtains, continuous selfing or backcrossing 1-3 times obtain stable long grain japonica rice
Two-line sterile line material；
Step 4) and 5) middle acquisition rice grain length >=6.5mm selection are two kinds: one in once hybridization segregating population
Middle selection has the rice single plant of long kernel Characters, it is desirable that selects in rice grain length >=6.5mm or more single plant, then reselection agronomy
The excellent single plant of character, obtaining long grain japonica rice two is sterile material；Two be that the long grain japonica rice material for obtaining primary election further returns
It hands over, polymerize other beneficial genes with long kernel Characters, obtaining long grain japonica rice two is sterile material；
6) light temperature culture identification (manually climate box identification length is carried out to the long grain japonica rice two-line sterile line material that step 5) obtains
Grain japonica rice two is the Fertility Transfer Temperature of sterile material), Xian round-grained rice assessment of indices (is measured with the molecular labeling of difference Xian round-grained rice difference
Be bred as the Indica or japonica of material, the selection of economical character meets Production of Large Fields requirement), round-grained rice sex index > 0.75, economical character choosing
It selects, to obtain long grain japonica rice two-line sterile line.
Application examples 1:
The cultivation of the long long round-grained rice 1S of grain Japonica Hybrid:
(1) it selects indica type two-line sterile line Peiai 64S to keep being that long round-grained rice 1B hybridizes with long grain japonica rice, is returned in the long round-grained rice 1B of its F1 generation
It hands over, the isolated F2-F4 of plantation hybridization from generation to generation, selects long two system's infertility single plant of kernel Characters, continuous to plant the stable length of 2-3 generation acquisition
Grain japonica rice two is sterile material.The identification of light temperature, the identification of Xian round-grained rice index, economical character selection are carried out by growth cabinet to it,
Cultivate the long round-grained rice 1S of long grain japonica rice two-line sterile line.
(2) long round-grained rice 1S stable fertility shows high temperature-induced sterility, and low temperature is fertile, and Fertility Transfer Temperature is 23.5 DEG C；Round-grained rice 1S is opened to open
Flower habit is good, stigma exposing ratio is high, outcrossing seed-setting rate is high；Through Xian round-grained rice assessment of indices, long round-grained rice 1S round-grained rice sex index 0.91, for typical round-grained rice
Rice；Long round-grained rice 1S long grain shape (rice grain length 7.2mm, length-width ratio 3.2), appearance and rice matter index are excellent, and breeding time is suitable, economical character
Excellent, field planting shows blast resisting.
(3) long round-grained rice 1S and self-fertile restorer combo, long grain Japonica Hybrid Combination nova (kind) cell of two systems of preparation are utilized
Experimental cultivation shows stable high yield, and rice grain length 7.1mm, blast resisting, economical character is excellent, prepares application and participates in provincial region
Test.
Application examples 2:
The cultivation of the long long round-grained rice 3S of grain Japonica Hybrid
(1) indica type two-line sterile line Y58S is selected to hybridize with the Derivative line D650 of long grain Restoring Line of Japonica Rice material L917, in its F1
Alternative D650 backcrossing, plantation hybridize isolated F2-F4 from generation to generation, select long two system's infertility single plant of kernel Characters, continuously plant 2-3 generation and obtain
Obtaining stable long grain japonica rice two is sterile material.The identification of light temperature, the identification of Xian round-grained rice index, agronomy are carried out by growth cabinet to it
Character determination cultivates the long round-grained rice 3S of long grain japonica rice two-line sterile line.
(2) long round-grained rice 3S stable fertility shows high temperature-induced sterility, and low temperature is fertile, and Fertility Transfer Temperature is 23.2 DEG C；Round-grained rice 1S is opened to open
Flower habit is good, stigma exposing ratio is high, outcrossing seed-setting rate is high；Through Xian round-grained rice assessment of indices, long round-grained rice 1S round-grained rice sex index 0.87, for typical round-grained rice
Rice；Long round-grained rice 3S long grain shape (rice grain length 7.5mm, length-width ratio 3.3), appearance and rice matter index are excellent, and breeding time is suitable, economical character
It is excellent, there is fragrance.
(3) long round-grained rice 3S and self-fertile restorer combo, long grain Japonica Hybrid Combination nova (kind) plot experiment of two systems of preparation
Planted stable high yield, economical character is excellent, and setting percentage is high, and exterior quality is all good with mouthfeel, shows preferable application prospect.
The long round-grained rice 1S grain shape of the long japonica rice two-line sterile line of table 1 and Main Agronomic characteristic
The long round-grained rice 3S grain shape of the long japonica rice two-line sterile line of table 2 and Main Agronomic characteristic
。
Claims (8)
1. a kind of long grain japonica rice two-line sterile line selection, it is characterised in that the following steps are included:
1) the long grain japonica rice that selection patent No. ZL 201210152095.X and patent No. ZL 201210152908.5 is related to restores
System and holding based material, as receptor or donor parents；
2) select the indica type two-line sterile line with excellent economical character as donor or receptor parent, the indica type two systems infertility
The sterile gene of system comes from Nongken 58S and An Nong S；
3) step 1) is hybridized with the rice material of step 2, is then returned using step 1) as male parent or female parent
It hands over, reestablish diplomatic relations 1-2 times, making long grain japonica rice gene and two is that sterile gene or other beneficial genes recombinate；
4) offspring is separated in several backcrossings that step 3) obtains, and selects two system's infertility single plant of rice grain length >=6.5mm or more
Harvest seed；
5) plantation step 4) the long grain japonica rice infertility single plant that obtains, continuous selfing or backcrossing 1-3 times obtain stable long grain japonica rice
Two-line sterile line material；
6) light temperature culture identification, Xian round-grained rice assessment of indices, round-grained rice are carried out to the long grain japonica rice two-line sterile line material that step 5) obtains
Index > 0.75, economical character selection, to obtain long grain japonica rice two-line sterile line.
2. a kind of long grain japonica rice two-line sterile line selection as described in claim 1, it is characterised in that the step 1)
Middle long grain Restoring Line of Japonica Rice is L917, L42 or their breeding Derivative line and intermediate materials, and keeping based material is long round-grained rice 1B, length
Round-grained rice 2B or their breeding Derivative line and intermediate materials.
3. a kind of long grain japonica rice two-line sterile line selection as described in claim 1, it is characterised in that the step 2)
In indica type two-line sterile line be Peiai 64S and Y58S.
4. a kind of long grain japonica rice two-line sterile line selection as described in claim 1, it is characterised in that the step 3)
Include the F2-F5 segregating generation once hybridized with 4) middle separation offspring, or once hybridizes the F2- for later and being reestablished diplomatic relations and being returned
F5 segregating generation.
5. a kind of long grain japonica rice two-line sterile line selection as described in claim 1, it is characterised in that the step 4)
With 5) in obtain rice grain length >=6.5mm selection be two kinds: one for once hybridization segregating population in select to have
The rice single plant of long kernel Characters, it is desirable that selected in rice grain length >=6.5mm or more single plant, then reselection economical character is excellent
Single plant, obtaining long grain japonica rice two is sterile material；Two be that the long grain japonica rice material for obtaining primary election is further returned, and has other
For niche because polymerizeing with long kernel Characters, obtaining long grain japonica rice two is sterile material.
6. a kind of long grain japonica rice two-line sterile line selection as described in claim 1, it is characterised in that the step 6)
Middle light temperature culture identification is that manually climate box identifies that long grain japonica rice two is the Fertility Transfer Temperature of sterile material, Xian round-grained rice index or
The measurement of round-grained rice sex index is to measure the Indica or japonica for being bred as material, the selection of economical character with the molecular labeling of difference Xian round-grained rice difference
Meet Production of Large Fields requirement.
7. a kind of long grain japonica rice two-line sterile line selection as described in claim 1, it is characterised in that the step 6)
It is sterile material that the long grain japonica rice two-line sterile line of middle completion, which is the novel long grain japonica rice two of one kind, and Fertility al-teration is stablized, round-grained rice
Index > 0.75 can apply for provincial technical appraisement, prepare the long grain Japonica Hybrid of two systems, develop for Hybrid breeding in commercial system.
8. a kind of long grain japonica rice two-line sterile line selection as described in claim 1, it is characterised in that the step 6)
Rice grain length >=6.5mm of the long grain japonica rice two-line sterile line of middle completion.
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| CN113661916B (en) * | 2021-06-29 | 2022-05-06 | 高邮周邶墩农业发展有限公司 | Breeding method of long-grain indica-japonica intermediate wide-affinity restorer line |
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