CN109287478A - A kind of long particle shape two-line sterile line of rice selection - Google Patents
A kind of long particle shape two-line sterile line of rice selection Download PDFInfo
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- CN109287478A CN109287478A CN201811512192.9A CN201811512192A CN109287478A CN 109287478 A CN109287478 A CN 109287478A CN 201811512192 A CN201811512192 A CN 201811512192A CN 109287478 A CN109287478 A CN 109287478A
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- particle shape
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention discloses a kind of selection of long particle shape two-line sterile line, step are as follows: selects long particle shape rice as donor parents, hybridizes with two-line sterile line；In offspring, single plant of the initial option length-width ratio greater than 3.8 harvests seed；When by the obtained main fringe young spike development of long particle shape seed progeny to 5 leaf phase with 23.5 DEG C low-temperature treatment 7 days; eliminate solid normal strain; retain solid poor or not solid strain Ratooning reproduction; in the 5 leaf phase of young spike development with 22 DEG C after low-temperature treatment 7 days; it moves under normal condition and grows; obtain the seed of belt length grain gene and plantation; it is selected by continuous multi-generation; the two-line sterile line progress fertility feature and coordinate force observation for growing that normal, economical character is excellent and length-width ratio is greater than 3.8 are selected, the two-line sterile line for growing normal, long particle shape is finally obtained.Selection of the invention is suitable for carrying out particle shape improvement to existing two-line sterile line, significantly improves rice exterior quality.
Description
Technical field
The invention belongs to rice breeding technology fields, and in particular to a kind of long particle shape two-line sterile line of rice selection.
Background technique
The Grain shape traits of rice and yield and quality are closely related, mainly include grain length, grain is wide, grain is thick, length-width ratio and length
Thickness rate etc..Grain length not only determines the appearance and milled quality of rice, and also has an impact to the mass of 1000 kernel of rice, therefore explain
The heredity of bright rice grain shape and development mechanism, and it is applied to breeding practice, facilitate raising and the rice quality of rice yield
Improvement.
In following long grain shape Japonica two line hybrid rice, we can not only make full use of the gene of the control grain length such as GS3, GL7,
It can be combined with molecule supplementary means simultaneously, polymerize the lines progeny of multiple yield heterosis genes, cultivate more high yield and high quality
New rice variety.Breeding practice also sufficiently proves, after the length for improveing seed, while guaranteeing yield, quality can also be obtained
To promotion.Following Japonica two line hybrid rice needs improvement grain shape emphatically, makes full use of the grain length related gene cloned, and expansion molecule is auxiliary
Breeding is helped, so to fully consider the sterile line combo for selecting specific grain shape in parent's breeding, combine plant type, to protect
Demonstrate,prove its yielding ability.
In this context, the selection for inventing a kind of long particle shape two-line sterile line of rice, can significantly improve existing two
It is the quality of sterile line, starts the frontier of long grain double-linear hybrid rice breeding germplasm innovation.
Summary of the invention
The object of the present invention is to provide a kind of selections of long particle shape two-line sterile line, it can improve existing two system not
The quality trait for being, breeding high-yield two-line hybrid rice new varieties are educated, and are used for the production of hybrid seeds production of two-line hybrid rice.
In order to reach above-mentioned technical purpose, the present invention is realized especially by following technical scheme:
A kind of selection of long particle shape two-line sterile line, comprising the following steps:
1) donor parents of the long particle shape rice germplasm material as grain length gene are selected, are hybridized with traditional two-line sterile line；
2) in hybridization separation offspring, single plant harvest length-width ratio is selected to be greater than 3.8 seed；
3) by the obtained main fringe young spike development of long particle shape seed progeny to 5 leaf phases, low-temperature treatment eliminates solid normal strain,
Retain solid poor or not solid strain Ratooning reproduction, in 5 leaf phase of young spike development low-temperature treatment, move under normal condition and grow, obtains
The seed of belt length grain gene；
4) obtained long grain gene seed is selected by continuous multi-generation, select that growth is normal, economical character is excellent and
Germplasm of the length-width ratio greater than 3.8 carries out fertility feature and coordinate force observation, while verifying validity, it is final obtain growth it is normal,
The germ plasm resource of long particle shape.
Further, long particle shape rice germplasm material described in step (1) be selected from japonica rice, long-grained nonglutinous rice, Xian round-grained rice hand over material or
One of java rice kind or a variety of.Preferably, the length-width ratio of the long particle shape rice germplasm material is greater than 3.8.
Further, the length-width ratio of traditional two-line sterile line germplasm described in step (1) is less than 3.0.
Further, low-temperature treatment condition described in step (2) is 22~23.5 DEG C.
Further, the belt length grain gene seed of acquisition described in step (3) is F3-F6 generation.
Further, germ plasm resource described in step (4) is temperature sensitive or photosensitive sterile line.Preferably, the germplasm
Resource is F7-F9 generation.
In the present invention, the long granular material that is obtained through initial option can again with the donor for carrying long grain gene or with tradition two
It is that parent is returned 1-2 times, is separated in offspring at it, single plant of the length-width ratio greater than 3.8 is further selected to harvest seed；It can also be again
It is reestablished diplomatic relations or is returned with other materials (japonica rice, long-grained nonglutinous rice, Xian round-grained rice hand over material or java rice) with excellent character, there is other
Niche further selects single plant of the length-width ratio greater than 3.8 to harvest seed because polymerizeing with Grain shape traits, then from its separation offspring.
In the present invention, repeatable step (3) (4) further verify its sterility and economical character, and it is excellent to obtain economical character
Good long particle shape two-line sterile line.
The method have the benefit that: the present invention by select suitable breeding material as parent, using hybridize,
It a variety of breeding methods, the long particle shape two-line sterile line of breeding such as reestablishes diplomatic relations or is returned, then its coordinate force is measured by test cross and is assembled miscellaneous
Hand over combination.Selection of the invention is suitable for carrying out particle shape improvement to existing two-line sterile line, and changing length-width ratio less than 3.0 is length
Wide ratio is greater than 3.8, significantly improves the particle shape of two-line hybrid rice, and then improves its exterior quality.
Specific embodiment
Below in conjunction with specific embodiment of the present invention, technical solution of the present invention is clearly and completely described, is shown
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
Embodiment 1
A kind of selection of long particle shape two-line sterile line of rice of the invention, comprising the following steps:
(1) long particle shape rice pest insects material, the donor parents as particle shape and property character gene are selected；
(2) donor parents for carrying long particle shape gene are hybridized, be returned or reestablished diplomatic relations with traditional two-line sterile line, make particle shape
Gene and other beneficial genes recombinate；
(3) seed is harvested in single plant of the separation Offspring F2-F5 selection length-width ratio greater than 3.8 of several Crossing systems；
(4) with 23.5 DEG C when by the main fringe young spike development of the single-strain seed offspring of long particle shape obtained in step (3) to 5 leaf phase
Low-temperature treatment 7 days, solid normal strain is eliminated, retains solid poor or not solid strain Ratooning reproduction, in the 5 leaf phase of young spike development with 22 DEG C
It after low-temperature treatment 7 days, moves under normal condition and grows, obtain the seed of belt length grain gene；
(5) seed that plantation step (4) obtains, selects by continuous multi-generation, selects that growth is normal, economical character is excellent
And two-line sterile line of the length-width ratio greater than 3.8 carries out fertility feature and coordinate force observation, while verifying validity, is finally given birth to
The two-line sterile line of long normal, long particle shape.
(6) seed obtained in step (5) measures its coordinate force from different restorer test crosses, selects wherein coordinate force
Strain high, comprehensive agronomy character is excellent；
(7) step (6) are repeated, verifies its economical character and coordinate force, it is new to obtain excellent two system of long particle shape of economical character
Sterile line.
Long particle shape rice pest insects material in above-mentioned steps (1) is japonica rice, long-grained nonglutinous rice, Xian round-grained rice friendship material or the java rice of grain length
Material.
Traditional two-line sterile line material in above-mentioned steps (2) is traditional two-line sterile line of the length-width ratio less than 3.0.
Length-width ratio is obtained in above-mentioned steps (5) has at least two greater than 3.8 two-line sterile line method: first is that primary miscellaneous
Hand over single plant of the selection with long kernel Characters in segregating population, it is desirable that length-width ratio is selected in the single plant greater than 3.8, then reselection plant type
And the single plant progress treatment of different temperature that economical character is excellent, obtain the long particle shape two-line sterile line of artificial modified；Second is that by first
It selects the elongated material obtained and long grain gene donor to be returned, then uses treatment of different temperature again, make it easier to obtain long particle shape
Two-line sterile line offspring.Wherein, the material with excellent character is japonica rice, long-grained nonglutinous rice, Xian round-grained rice friendship material and java rice.
The two-line sterile line being previously mentioned in above-mentioned steps is photosensitive or temperature sensitive type two-line sterile line；
Above-mentioned steps (5), (6), (7) middle long particle shape two-line sterile line obtained, length-width ratio are greater than 3.8, can be used for preparing
Two-line hybrid rice new varieties can also carry out the production of hybrid seeds production of Hybrid Rice Combination.
Embodiment 2
The breeding of long particle shape two-line sterile line richness 7S: using the long grain round-grained rice of long particle shape rice material be donor, Peiai 64S be by
Body is hybridized, then with the F1 generation of Peiai 64S/ long grain round-grained rice to be maternal, and Guangzhan 63S is that male parent is reestablished diplomatic relations, in Offspring F2-F5
The Single-plant selection of warm (light) quick character and particle shape and character is carried out from generation to generation.F8 generation with extensive 8006 in indica restorer line, in it is extensive
8012, extensive 8015,93-11, Huang Hua such as account at the test crosses in, and by the identification and screening to test cross F1 generation, final choice is to 1 part two
It is sterile line richness 7S.This part of two-line sterile line show as long grain, the features such as comprehensive agronomy character is good, coordinate force is high, sterile line
Origin temp is suitable with parent's Guangzhan 63S.The two-line hybrid rice Combination nova richness 3S/R306 assembled, grain is long, quality is excellent and comprehensive
Agronomic is preferable, and cell cuts yield in fact and reaches 684.3kg, rich by two excellent No. four higher by 6.9% than compareing, and reaches the level of signifiance.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understand without departing from the principles and spirit of the present invention can to these examples carry out it is a variety of variation, modification, replacement and
Modification, the scope of the present invention is defined by the appended.
Claims (8)
1. a kind of selection of long particle shape two-line sterile line, which comprises the following steps:
1) donor parents of the long particle shape rice germplasm material as grain length gene are selected, are hybridized with traditional two-line sterile line；
2) in hybridization separation offspring, single plant harvest length-width ratio is selected to be greater than 3.8 seed；
3) by the obtained main fringe young spike development of long particle shape seed progeny to 5 leaf phases, low-temperature treatment eliminates solid normal strain, retains
Solid poor or not solid strain Ratooning reproduction is moved under normal condition and is grown in 5 leaf phase of young spike development low-temperature treatment, obtains belt length
The seed of grain gene；
4) obtained long grain gene seed is selected by continuous multi-generation, selects that growth is normal, economical character is excellent and length and width
Fertility feature and coordinate force observation are carried out than the germplasm greater than 3.8, while verifying validity, final obtain grows normal, long grain
The germ plasm resource of shape.
2. a kind of selection of long particle shape two-line sterile line according to claim 1, which is characterized in that in step (1)
The long particle shape rice germplasm material is selected from japonica rice, long-grained nonglutinous rice, Xian round-grained rice and hands over one of material or java rice kind or a variety of.
3. a kind of selection of long particle shape two-line sterile line according to claim 2, which is characterized in that the long grain
The length-width ratio of shape rice germplasm material is greater than 3.8.
4. a kind of selection of long particle shape two-line sterile line according to claim 1, which is characterized in that in step (1)
The length-width ratio of traditional two-line sterile line germplasm is less than 3.0.
5. a kind of selection of long particle shape two-line sterile line according to claim 1, which is characterized in that in step (2)
The low-temperature treatment condition is 22~23.5 DEG C.
6. a kind of selection of long particle shape two-line sterile line according to claim 1, which is characterized in that in step (3)
The belt length grain gene seed of the acquisition is F3-F6 generation.
7. a kind of selection of long particle shape two-line sterile line according to claim 1, which is characterized in that in step (4)
The germ plasm resource is temperature sensitive or photosensitive sterile line.
8. a kind of selection of long particle shape two-line sterile line according to claim 7, which is characterized in that the germplasm
Resource is F7-F9 generation.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811512192.9A CN109287478A (en) | 2018-12-11 | 2018-12-11 | A kind of long particle shape two-line sterile line of rice selection |
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| CN201811512192.9A CN109287478A (en) | 2018-12-11 | 2018-12-11 | A kind of long particle shape two-line sterile line of rice selection |
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| CN109287478A true CN109287478A (en) | 2019-02-01 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112514787A (en) * | 2020-11-06 | 2021-03-19 | 湖南农业大学 | Breeding method of rice long-small grain type two-line sterile line and simple seed production method of hybrid rice |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112514787A (en) * | 2020-11-06 | 2021-03-19 | 湖南农业大学 | Breeding method of rice long-small grain type two-line sterile line and simple seed production method of hybrid rice |
| CN112514787B (en) * | 2020-11-06 | 2022-06-07 | 湖南农业大学 | Breeding method of rice long-small grain type two-line sterile line and simple seed production method of hybrid rice |
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