CN113661916A - Breeding method of long-grain indica-japonica intermediate wide-affinity restorer line - Google Patents
Breeding method of long-grain indica-japonica intermediate wide-affinity restorer line Download PDFInfo
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- CN113661916A CN113661916A CN202110726780.8A CN202110726780A CN113661916A CN 113661916 A CN113661916 A CN 113661916A CN 202110726780 A CN202110726780 A CN 202110726780A CN 113661916 A CN113661916 A CN 113661916A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
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Abstract
The invention relates to a breeding method of a long-grain indica-japonica intermediate wide-affinity restorer line, which comprises the following steps: taking long-grain indica conventional rice, restorer line or maintainer line or other long-grain rice lines, and hybridizing with indica-japonica intermediate wide-affinity restorer line to obtain F1Seed generation and selfing for the first generation F2Seed generation and planting F2Isolating the population, observing2Separating the grain type of each individual plant of the population, selecting long-grain type individual plants with good plant leaf morphology from the individual plants, observing and recording various obtained grain types, comparing the field phenotype identification, picking out long-grain type plants with plant leaves different from japonica rice or indica rice, harvesting seeds, continuously selfing for multiple generations, selecting the grain type and plant leaf morphology of each individual plant of each plant line for each generation, and obtaining the long-grain type indica rice intermediate type wide-compatibility recovery with stable heredityAnd (4) repeating the steps. Simple and practical, can simplify the breeding steps, can be used for preparing partially indica type indica rice and japonica rice interspecies hybrid rice, and meets the requirements of different regions for planting rice with different tastes.
Description
Technical Field
The invention relates to a breeding method of a long-grain indica-japonica intermediate wide-affinity restorer line, belonging to the technical field of rice breeding.
Background
The utilization of heterosis greatly improves the yield per unit of rice in China, and the current planting area of hybrid rice accounts for about 50 percent of the total planting area of rice in China. The breeding of new hybrid rice varieties is the original power of the development of the hybrid rice industry, and the early hybrid rice varieties bred in China mainly comprise subspecies intraspecific hybrid rice and are mainly concentrated in subspecies of indica type; because the problems of affinity and the like of the interspecific hybrid rice are solved, and the great advantages in the aspects of yield, resistance and the like are also adopted, more and more new varieties of the indica-japonica interspecific hybrid rice are approved in recent years, the utilization level of the rice heterosis in China is further improved, the further development of the hybrid rice industry in China is promoted, a new breeding stage is also predicted to come, and the current stage is just in a transition stage, namely the stage of transition from the subspecies internal hybridization to the indica-japonica interspecific hybridization.
At present, a plurality of new indica-japonica hybrid rice varieties are independently or jointly bred in middle and lower reaches of Yangtze river, in particular in a plurality of breeding units and seed enterprises in Zhejiang province, wherein the new varieties are represented by Yongyou series varieties bred by Ningbo city agricultural academy and spring-you series varieties bred by Chinese rice institute, and are currently in the stage of popularization and application.
As the research of the indica-japonica subspecies hybrid rice is late, the prior indica-japonica subspecies hybrid rice is mainly matched in a japonica non-indica recovery mode, and most of the hybrid rice utilizes a three-line hybrid system, the varieties of the two-line hybrid indica-japonica subspecies hybrid rice are less, and the two-line sterile lines are indica sterile lines. Most of restorers of indica-japonica subspecies hybrid rice are indica-japonica intermediate type partially indica type restorers with wide affinity bred by indica-japonica introgression hybridization, most of grain types of the restorers are between a cluster grain type of typical japonica rice and a long grain type of typical indica rice, the aspect ratio is about 2.4, the long grain type restorers (the aspect ratio is more than 3) are few, and the production requirement of grain type diversification cannot be met.
Disclosure of Invention
The purpose of the invention is as follows: aiming at the existing problems and defects, the invention aims to provide a breeding method of a long-grain indica-japonica intermediate wide-affinity restorer line, more grain types are increased by the method, diversified grain type requirements are met,
the technical scheme is as follows: in order to realize the purpose, the invention adopts the following technical scheme:
a breeding method of a long-grain indica-japonica intermediate wide-affinity restorer line comprises the following steps:
step 1: hybridizing one of long-grain indica conventional rice, restorer line or maintainer line with indica-japonica intermediate wide-affinity restorer line to obtain F1；
Step 2: f obtained in step 11Selfing the first generation, observing and recording various different grain types;
and step 3: comparing the various different grain types obtained in the step 2 with field phenotype identification, and picking out single plants which are long-grain type and have plant leaves different from japonica rice or indica rice;
and 4, step 4: and (4) continuously selfing the single plants obtained in the step (3) for multiple generations, and selecting the single plants with long grain type and excellent plant leaf properties to obtain the long grain type indica-japonica intermediate wide-affinity restoring line.
Further, the long particle type is a particle type having an aspect ratio of more than 3.
Further, the long-grain indica restorer line in the step 1 is used as a male parent to be hybridized with the indica-japonica intermediate wide-affinity restorer line.
Further, the long-grain indica-japonica intermediate wide-affinity restorer line obtained in claim 1 is hybridized with one of a charpy type, a yunnan type or a patrinia type, and the setting rate is more than 80%.
Further, the long-grain indica-japonica intermediate wide-affinity restorer line obtained in the claim 1 is hybridized with the indica two-line sterile line, and the maturing rate is more than 80%.
Has the advantages that: compared with the prior art, the invention has the following advantages: the method is a novel breeding method of the long-grain indica-japonica intermediate wide-affinity restorer line, can simplify breeding steps, obtains long-grain japonica rice, and meets the requirements of different tastes in different regions.
Detailed Description
The present invention is further illustrated by the following examples, which are intended to be purely exemplary and are not intended to limit the scope of the invention, as various equivalent modifications of the invention will occur to those skilled in the art upon reading the present disclosure and fall within the scope of the appended claims.
Examples
Taking long-grain indica restorer line T301 as male parent to hybridize with indica-japonica intermediate wide-affinity restorer line CH15 to obtain F1And (5) seed generation. F obtained1And (4) selfing the first generation, and observing and recording various obtained different grain types. And (4) comparing the obtained various grain types and carrying out phenotype identification on the field, and picking out single plants with long grain types and plant leaves different from those of japonica rice or indica rice. And (3) continuously selfing the obtained single plant for multiple generations, and selecting the single plant with long grain type and excellent plant leaf properties to obtain the long grain type indica-japonica intermediate type wide-affinity restoring line. The long-particle type is a particle type having an aspect ratio of more than 3. The long-grain indica-japonica intermediate wide-affinity restorer is hybridized with one of the Baotai type, the Dian type or the Valeriana type, and the setting rate is more than 80 percent. The obtained long-grain indica-japonica intermediate wide-affinity restorer line and indica two-line sterile line hybridThe maturing rate is also more than 80%.
However, most of the existing cultivation methods can only obtain japonica rice meeting the requirements of grain type, and can not obtain japonica rice which simultaneously meets the requirements of indica type and intermediate type and is a wide-affinity restorer line, and if the existing cultivation methods simultaneously meet the requirements of adding various medicines or having requirements on various environments, the existing cultivation methods can not be suitable for various environments.
Therefore, the long-grain indica-japonica intermediate wide-affinity restorer line obtained by the method has high seed set rate, provides more seeds for subsequent cultivation or use, has excellent properties, meets the requirements of the long-grain indica-japonica intermediate wide-affinity restorer lines with various grains, can be applied to various environments, and is not affected by cultivation of the high-quality long-grain restorer line meeting the requirements. Compared with the existing simple cultivation method, the method can not meet various requirements, is competitive and is more convenient.
Claims (5)
1. A breeding method of a long-grain indica-japonica intermediate wide-affinity restorer line is characterized in that: the method comprises the following steps:
step 1: hybridizing one of long-grain indica conventional rice, restorer line or maintainer line with indica-japonica intermediate wide-affinity restorer line to obtain F1；
Step 2: f obtained in step 11Selfing the first generation, observing and recording various different grain types;
and step 3: comparing the various different grain types obtained in the step 2 with field phenotype identification, and picking out single plants which are long-grain type and have plant leaves different from japonica rice or indica rice;
and 4, step 4: and (4) continuously selfing the single plants obtained in the step (3) for multiple generations, and selecting the single plants with long grain type and excellent plant leaf properties to obtain the long grain type indica-japonica intermediate wide-affinity restoring line.
2. The breeding method of the long-grain indica-japonica intermediate wide-affinity restorer line according to claim 1, which is characterized in that: the long particle type is a particle type with an aspect ratio larger than 3.
3. The breeding method of the long-grain indica-japonica intermediate wide-affinity restorer line according to claim 1, which is characterized in that: and (3) hybridizing the long-grain indica restorer line in the step (1) serving as a male parent with the indica-japonica intermediate wide-affinity restorer line.
4. The breeding method of the long-grain indica-japonica intermediate wide-affinity restorer line according to claim 1, which is characterized in that: the long-grain indica-japonica intermediate wide-affinity restorer line obtained in claim 1 is hybridized with one of a charpy type, a yunnan type or a patrinia type, and the setting rate is more than 80%.
5. The breeding method of the long-grain indica-japonica intermediate wide-affinity restorer line according to claim 1, which is characterized in that: the long-grain indica-japonica intermediate wide-affinity restorer line obtained in the claim 1 is hybridized with an indica two-line sterile line, and the maturing rate is more than 80%.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577933A (en) * | 2012-03-20 | 2012-07-18 | 安徽绿亿种业有限公司 | Method for breeding indica sterile line-japonica restorer line intersubspecific hybrid rice and application of method |
| CN104737898A (en) * | 2015-04-02 | 2015-07-01 | 福建金山都发展有限公司 | Fast creating method for rice long-grain japonica type indica-compatible two-line sterile line germplasm |
| CN109287475A (en) * | 2018-12-11 | 2019-02-01 | 中国水稻研究所 | A kind of long grain Japonica Hybrid selection of two systems |
| CN109349102A (en) * | 2018-12-11 | 2019-02-19 | 中国水稻研究所 | A kind of long grain japonica rice two-line sterile line selection |
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2021
- 2021-06-29 CN CN202110726780.8A patent/CN113661916B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577933A (en) * | 2012-03-20 | 2012-07-18 | 安徽绿亿种业有限公司 | Method for breeding indica sterile line-japonica restorer line intersubspecific hybrid rice and application of method |
| CN104737898A (en) * | 2015-04-02 | 2015-07-01 | 福建金山都发展有限公司 | Fast creating method for rice long-grain japonica type indica-compatible two-line sterile line germplasm |
| CN109287475A (en) * | 2018-12-11 | 2019-02-01 | 中国水稻研究所 | A kind of long grain Japonica Hybrid selection of two systems |
| CN109349102A (en) * | 2018-12-11 | 2019-02-19 | 中国水稻研究所 | A kind of long grain japonica rice two-line sterile line selection |
Non-Patent Citations (3)
| Title |
|---|
| 华宇峰等: "滇型晚粳稻不育系春江29A的选育及应用", 《杂交水稻》 * |
| 王建军等: "水稻广亲和恢复系92-7的选育和利用研究", 《浙江农业学报》 * |
| 董文其等: "水稻T301的广亲和性鉴定及其利用", 《浙江农业科学》 * |
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