CN1054700A - Practical hybrid seed production technology for high-yield and high-quality radishes - Google Patents
Practical hybrid seed production technology for high-yield and high-quality radishes Download PDFInfo
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- CN1054700A CN1054700A CN90101760A CN90101760A CN1054700A CN 1054700 A CN1054700 A CN 1054700A CN 90101760 A CN90101760 A CN 90101760A CN 90101760 A CN90101760 A CN 90101760A CN 1054700 A CN1054700 A CN 1054700A
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- 235000006140 Raphanus sativus var sativus Nutrition 0.000 title claims abstract description 24
- 238000005516 engineering process Methods 0.000 title claims abstract description 9
- 241000220259 Raphanus Species 0.000 title claims abstract 8
- 238000004519 manufacturing process Methods 0.000 title claims description 14
- 241000196324 Embryophyta Species 0.000 claims abstract description 19
- 238000000034 method Methods 0.000 claims abstract description 12
- 238000009396 hybridization Methods 0.000 claims abstract description 7
- 238000012423 maintenance Methods 0.000 claims description 18
- 238000009395 breeding Methods 0.000 claims description 13
- 230000001488 breeding effect Effects 0.000 claims description 13
- 238000010899 nucleation Methods 0.000 claims description 9
- 238000012360 testing method Methods 0.000 claims description 9
- 244000025254 Cannabis sativa Species 0.000 claims description 2
- 239000005321 cobalt glass Substances 0.000 claims description 2
- 230000009466 transformation Effects 0.000 claims description 2
- 238000000844 transformation Methods 0.000 claims 1
- 206010021929 Infertility male Diseases 0.000 abstract description 3
- 208000007466 Male Infertility Diseases 0.000 abstract description 3
- 239000003086 colorant Substances 0.000 abstract 1
- 244000088415 Raphanus sativus Species 0.000 description 16
- 239000000463 material Substances 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 238000012216 screening Methods 0.000 description 4
- 230000035558 fertility Effects 0.000 description 3
- 208000000509 infertility Diseases 0.000 description 3
- 230000036512 infertility Effects 0.000 description 3
- 208000021267 infertility disease Diseases 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 230000008774 maternal effect Effects 0.000 description 3
- 238000013459 approach Methods 0.000 description 2
- 210000000805 cytoplasm Anatomy 0.000 description 2
- 230000001086 cytosolic effect Effects 0.000 description 2
- 230000010152 pollination Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 241001674939 Caulanthus Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 235000005733 Raphanus sativus var niger Nutrition 0.000 description 1
- 240000001970 Raphanus sativus var. sativus Species 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000002153 concerted effect Effects 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000009395 genetic defect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
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- 238000006467 substitution reaction Methods 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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Abstract
A practical hybrid technology for preparing high-yield and high-quality radish seeds features that the male sterility principle is used to search the male sterile plants from golden flower moss, green round crisp, Tangshanhong and Jinfeng, and the male sterile plants are used to selectively culture male sterile line and maintainer line for hybridization. The yield of the hybrid seeds prepared by the method is improved by 30 percent. The method breeds 15 new hybrid varieties of first generation with different maturity, different purposes and different skin colors.
Description
The present invention relates to a kind of method of radish cross-breeding technology, international Patent classificating number is A01H1/02.
Through consulting CAB Abstracts (code name 53 storehouses) 1972-1983, world patent (code name 350 storehouses) 1963-1980, agricultural documentation index (code name 10 storehouses) 1979-1988, agricultural documentation index (code name 110 storehouses) 1970-1978, only detect 2 pieces in 53 storehouses and 10 storehouses, one is: the research of cytoplasmic male sterility behind rape and the radish intermolecular hybrid (the European crucifer annual meeting in 1979 of originating); It two is: adopt (radish and oily seed collecting intermolecular hybrid) " Ogura " male sterile material and introduce the first generation product of scheme (source: journal article " vegetables production science and environmental science " second the 9th phase of volume of nineteen eighty-two) from the radish to oil, above result shows, does not see reporting for work of radish nuclear-cytoplasmic male sterilty and application in patent documentation.In addition, we have consulted non-patent literature both domestic and external in the last thirty years, and the result is as follows: the Okumaso(1951 of (1) Japan) and Nishi(1958) found the male sterile of nuclear gene radish once, by the heredity of recessive nuclear gene control sterility.(2) Ri Ben Ogura(1968) find, but because bad and some other genetic defect of the economic characters of these materials is not used for the seed production of F1 so far by cytoplasmic sterility gene and the common male sterile material of controlling of genic male sterile gene.(3) Fa Guo Bonne(1975,1977) the Ogura sterile material of Japan is introduced France, though bred the production of hybrid seeds that male sterile line is used for F1, there are outstanding problems such as seed production is low in transformation on the precocious radish in Europe, has limited to enlarge and uses.
Radish is one of domestic and international important vegetables, and its hybrid vigour is very remarkable.But because its floral organ is little, single pod is set seeds few, can not produce the F1 seed with artificial cross and be used for producing.Therefore, seek the key issue that feasible seeding technique approach becomes heterosis utilization.Utilizing the self incompatible line production of hybrid seeds is a feasible approach, and the problem that exists single pod that is radish set seeds far beyond adopt in vain, wild cabbage is few, self incompatible line relies on artificial bud pollination reproductive-cost too high.In order to reduce breeding cost, improve seed production, Japan and other countries adopts four-way cross preparation double cross hybrid, but a double cross hybrid needs 4 different self incompatible lines of genotype of seed selection, work Cheng Xu complexity, and the double cross hybrid regularity is also undesirable.
The objective of the invention is to utilize the male sterility of radish, breed adaptable radish male sterile, can be used for preparing the radish half-blood and the saving of work and time of high yield and high quality.
Content of the present invention: the present invention utilizes nuclear-kytoplasm to make the selection principle of male sterile line mutually, Chinese radish (R.sativus L.lar.Longipinnatus Baileg) landrace golden flower tongue, blue or green circle is crisp, Tangshan is red, found the male sterile source in a plurality of kinds such as Jin Feng, adopt extensive test cross, male parent strain selfing, continuous backcross, the method of selecting, carry out the screening of homotype and special-shaped maintenance line, bred 48A, 77-01A, 262A, 4-01A, 7416A, 211A, 64A, complete blue or green A, 01-11A, the male sterile line and the maintenance line of ten different ecological type such as 4-02A.
Whole seed selection process is as follows:
One, seeks sterile strain: from the golden flower tongue, blue or green circle is crisp, Tangshan is red, any one can both search out sterile strain four Chinese varieties such as Jin Feng, sterile strain by this four strains, the sterile line material that seed selection forms, sterile degree height (sterile plant rate reaches 100%) stabilization characteristics of genetics, economic characters are good.
Two, the process of seed selection male sterile line and maintenance line: the process of seed selection male sterile line is actually the process of screening maintenance line:
1, the screening of homotype maintenance line:
(1) be maternal with the male sterile plant, to be male parent with some normal fertile strains in the kind, match some test cross combinations, each male parent strain selfing keeps.
(2), respectively plant the self progeny of each test cross combination and each male parent, the florescence is observed the fertility separation of each test cross combination, therefrom select the high combination of sterile plant rate, and its corresponding male parent male sterile plant do not occur otherwise eliminated.Illustrate then that because of sterile strain appears in male parent its cytoplasm may be sterile cytoplasm, can not breed and have the maintenance line that to educate kytoplasm and sterile nucleus gene.
(3), be female parent with the good sterile strain of flowering traits, with among the choosing combination male parent self progeny a plurality of plant make male parent, backcross, each male parent continues selfing respectively and keeps.
Self progeny, the florescence of planting respectively backcross combination and each male parent (4), are respectively observed the fertility performance of the combination of respectively backcrossing, and therefrom select the sterile plant rate height, and sterile plant rate can reach 100% combination.
(5), be female parent with the sterile strain in the combination of backcrossing, with in the choosing more individual plant of backcrossing among the combination male parent self progeny make male parent, proceed to backcross, the male parent strain continues the selfing reservation.
(6) through continuous backcross 3-4 generation, the continuous selfing 4-5 of its corresponding male parent generation.The combination of therefrom selecting with 100% sterile plant rate is the male sterile line of breeding, and its corresponding male parent is then to be the maintenance line of male sterile line.Accompanying drawing 1 has been enumerated the seed selection of 48A, has illustrated that promptly this seed selection crosses.
2, the screening of special-shaped maintenance line:
Make female parent with the male sterile plant of discovery or the male sterile line plant of having bred, make male parent with the normal fertile strain of different kind, match some test crosses combinations, each male parent strain selfing keeps.
The seed selection program is the same.In continuous backcross 4-5 generation,, its male parent involves continuous selfing 5-6 generation in a criminal case, also can filter out the special-shaped maintenance line with 100% hold facility.Because the result of continuous the backcrossing of accounting-nucleus substitution, but the main economic characters of its corresponding male sterile line and maintenance line basically identical.
Three, the seed selection of the first generation of hybrid
With the male sterile of breeding is maternal, the dissimilar selfing good with economic characters is male parent, prepare some hybrid combinations, by to the evaluation of integral economic traits such as each hybrid combination output, quality, disease resistance, select, determine that coordinate force is good, economic characters meet the fine combination of breeding objective requirement, through variety comparative test, after the regional test, a good first generation of hybrid is promptly bred.The male sterile line that utilizes the present invention to breed, bred Lu Luobu No. three, red rich No. one, red rich No. two, a Feng Guang generation, rich 15 the trailing plants first generation of hybrid new varieties such as a generation, a Feng Lu generation, rich a new generation, red No. one of spring, red No. two of spring, (48A * shell) F1, (complete blue or green A * Dandong smalt) F1, (7416A * 66) F1, (77-01A * Weihe River county green grass or young crops) F1, (77-01A * Guangdong late radish) F1, (give birth to 48A * U.S. dense morning) F1 of sticking up.
Because trailing plants F1 is a product with nutrition organs-fleshy root, pass through combining ability test, select good hybrid combination, whether its male parent system recovers is unimportant, so, utilize male sterile line to do maternal preparation F1 generation, as long as have two to be (male sterile line, maintenance line) and male parent system that excellent fit makes a concerted effort is arranged, do not need recovery to be.
Four, hybrid seeding
(1), male sterile line and male parent system breeds
Seed selection has good isolation condition seed collecting field and sets up male sterile line propagation and educate the garden.Male sterile line and the strain of maintenance line kind are planted according to 3: 1 ratio.And take: become the strain strict impurity elimination of reserving seed for planting; Male sterile line and the strain of maintenance line kind perform mark, prevent entanglement; Initial bloom stage check male sterile line fertility, pull out individually and can educate strain; Behind the seed maturity, male sterile line and maintenance line are strict divides measure such as receipts, guarantee the purity of breeding of male sterile line.Male parent system can adopt into the strain method of reserving seed for planting, and in the good isolation district, breeds.
(2) hybrid seeding
Set up the hybrid seeding garden in the good isolation district, adopt partly to become the strain method of reserving seed for planting, the male parent system of male sterile line and crossbreed planted by 4: 1.For preventing that gathering in the crops mistiming is hybrid income hybrid the inside with male parent, behind the seed farm full-bloom stage, kind of the strain of male parent system can be pulled out, can guarantee that like this gained seed is 100% crossbreed.
The different ecological type that the present invention bred, the male sterile line that proterties is good, sterility is stable, sterile plant rate, sterile degree and production of hybrid seeds hybrid rate all can reach 100%, and have good coordinate force, for the radish heterosis utilization has been created a science and cost-effective figure footpath.Billy of the present invention has following advantage with the self incompatible line production of hybrid seeds: (1) reproductive-cost is low, and promotion rate is fast.Male sterile line can large tracts of land be bred in natural isolated area, does not need artificial pollination, and general mu seed yield is about 50 kilograms, and the production cost of its original seed only is about 1/10 of a self incompatible line original seed.And be easy to breeding, and not being subjected to the restriction of artificial and facility condition, can breed as required.Therefore, hybrid seeding is convenient to carry out, and the popularization in F1 generation is also fast.(2) hybrid rate height, seed quality is guaranteed.The male sterile line that the present invention breeds, the hybrid rate when sterile plant rate and hybrid seeding all can reach 100%, and utilizes the self incompatible line production of hybrid seeds, and reasons such as the interior brother and sister's friendship of system take place owing to meeting, and the hybrid rate can not reach 100%.(3) hybrid seeding output height (generally improving 30%), first generation of hybrid kind is stable.The breeding of male sterile line is and the pangamy of maintenance line large group, does not have artificial pressure selfing.Therefore, do not resemble continuous selfing the self incompatible line and cause the vitality decline.So, utilize the male sterile line production of hybrid seeds, the seed production height, the kind of the first generation of hybrid is also more stable.
Claims (5)
1, a kind of technology for breeding high-yield and quality radish by hybridisation, it is characterized in that using the production of hybrid seeds of male sterile method, specifically comprise following step: (1) seeks sterile strain, the seed selection of (2) male sterile line and maintenance line, (3) seed selection of the first generation of hybrid, (4) hybrid seeding.
2, a kind of technology for breeding high-yield and quality radish by hybridisation according to claim 1, it is characterized in that seeking sterile strain the golden flower tongue, blue or green circle is crisp, Tangshan is red, seed selection in the rich four strains in Shanxi.
3, a kind of technology for breeding high-yield and quality radish by hybridisation according to claim 1 is characterized in that seed selection male sterile line and maintenance line are to take extensive test cross, male parent strain selfing, the system of selection of continuous backcross.
4, a kind of technology for breeding high-yield and quality radish by hybridisation according to claim 1, the seed selection that it is characterized in that the first generation of hybrid need not recover.
5, a kind of technology for breeding high-yield and quality radish by hybridisation according to claim 1 is characterized in that hybrid seeding is to utilize the golden flower tongue, blue or green circle is crisp, Tangshan is red, male sterile line that the sterile strain of the rich four strains in Shanxi is bred and the male sterile line that becomes with their transformations: 48A thereof, 77-01A, 262A, 4-01A, 7416A, 211A, 64A, complete blue or green A, 01-11A, the male sterile line of ten different ecological type such as 4-02A and maintenance line thereof are cultivated Lu Luobu No. three, red rich No. one, red rich No. two, a Feng Guang generation, the rich generation of sticking up, a Feng Lu generation, rich a new generation, red No. one of spring, red No. two of spring, (48A * shell) F1, (complete blue or green A * Dandong smalt) F1, (7416A * 66) F1, (77-01A * Weihe River county green grass or young crops) F1, (77-01A * Guangdong late radish) F1,15 trailing plants first generation of hybrid new varieties such as (give birth to 48A * U.S. dense morning) F1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN90101760A CN1020390C (en) | 1990-03-07 | 1990-04-02 | Practical hybrid seed production technology for high-yield and high-quality radishes |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN90105048A CN1028590C (en) | 1990-03-07 | 1990-03-07 | Technology method of stereoscopic strawberry growing without soil |
| CN90101760A CN1020390C (en) | 1990-03-07 | 1990-04-02 | Practical hybrid seed production technology for high-yield and high-quality radishes |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 92110368 Division CN1075052A (en) | 1992-09-11 | 1992-09-11 | Hybridisation technique for high-yield and high quality turnip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1054700A true CN1054700A (en) | 1991-09-25 |
| CN1020390C CN1020390C (en) | 1993-05-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN90101760A Expired - Fee Related CN1020390C (en) | 1990-03-07 | 1990-04-02 | Practical hybrid seed production technology for high-yield and high-quality radishes |
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| CN (1) | CN1020390C (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072889C (en) * | 1996-11-28 | 2001-10-17 | 中国农业科学院蔬菜花卉研究所 | Tech. For breeding and prodn. of hybrid seeds of wild cabbage dominance male sterile line |
| CN1109494C (en) * | 1999-09-08 | 2003-05-28 | 民权县民权种业有限公司 | Breeding method for radish model Deri 2 |
| CN1332022C (en) * | 2002-10-10 | 2007-08-15 | 株式会社农友Bio | Radish-series callosum and method for culturing plant and hybrid by using same |
| CN103651102A (en) * | 2013-12-06 | 2014-03-26 | 重庆市涪陵区农业科学研究所 | Seed production technology for carmine No. 1 red-peel red-pulp radish |
| CN105532431A (en) * | 2015-12-18 | 2016-05-04 | 山东省潍坊市农业科学院 | Hybrid seed production method of organic turnip |
| CN110896849A (en) * | 2019-12-12 | 2020-03-24 | 重庆市渝东南农业科学院 | High-sterility-based carmine radish seed cultivation method |
| CN112450021A (en) * | 2020-12-07 | 2021-03-09 | 江苏沿海地区农业科学研究所 | Method for rapidly detecting purity of radish sterile line |
-
1990
- 1990-04-02 CN CN90101760A patent/CN1020390C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072889C (en) * | 1996-11-28 | 2001-10-17 | 中国农业科学院蔬菜花卉研究所 | Tech. For breeding and prodn. of hybrid seeds of wild cabbage dominance male sterile line |
| CN1109494C (en) * | 1999-09-08 | 2003-05-28 | 民权县民权种业有限公司 | Breeding method for radish model Deri 2 |
| CN1332022C (en) * | 2002-10-10 | 2007-08-15 | 株式会社农友Bio | Radish-series callosum and method for culturing plant and hybrid by using same |
| CN103651102A (en) * | 2013-12-06 | 2014-03-26 | 重庆市涪陵区农业科学研究所 | Seed production technology for carmine No. 1 red-peel red-pulp radish |
| CN105532431A (en) * | 2015-12-18 | 2016-05-04 | 山东省潍坊市农业科学院 | Hybrid seed production method of organic turnip |
| CN110896849A (en) * | 2019-12-12 | 2020-03-24 | 重庆市渝东南农业科学院 | High-sterility-based carmine radish seed cultivation method |
| CN110896849B (en) * | 2019-12-12 | 2021-08-06 | 重庆市渝东南农业科学院 | High-sterility-based carmine radish seed cultivation method |
| CN112450021A (en) * | 2020-12-07 | 2021-03-09 | 江苏沿海地区农业科学研究所 | Method for rapidly detecting purity of radish sterile line |
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| Publication number | Publication date |
|---|---|
| CN1020390C (en) | 1993-05-05 |
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