CN1072889C - Tech. For breeding and prodn. of hybrid seeds of wild cabbage dominance male sterile line - Google Patents
Tech. For breeding and prodn. of hybrid seeds of wild cabbage dominance male sterile line Download PDFInfo
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Abstract
In the present invention, by the sensitivity of an utilizing cabbage dominant karyotype male sterility gene CDMs399-3 under certain genic environment conditions, the homozygous dominant sterile lines of the cabbage dominant karyotype male sterility gene CDMs399-3 are selected and bred by a self cross, a back cross and a test cross; the homozygous dominant sterile lines are reproduced by the tissue culture and the preservation. By the test cross, temporary maintainer lines are sieved, and the temporary maintainer lines hybridize with the homozygous dominant sterile lines so that a sterile line with the sterile strain rate of 100%, the sterile degree of 100% or not less than 95% is obtained. The sterile line hybridizes with self-cross lines with a good combining ability so that a first filial generation is prepared. The method had the advantages that the crossing rate of the seeds of the first filial generation can reach 100% when seeds are produced by hybridization, the parental original seeds do not need artificial pollination, thereby lowering the seed production cost, etc.
Description
The present invention relates to the seed selection and the seeding technique thereof of wild cabbage (Brassica oleracea L.var.capitata L.) dominance genetic male sterile line, belong to wild cabbage heterosis utilization field.
Heterosis utilization is a kind of main breeding approach of crop in cruciferae such as Chinese cabbage, wild cabbage.For a long time, all be the method preparation cabbage hybrid that utilizes self incompatible line both at home and abroad.States such as Japan and the United States just utilize self incompatible line preparation cabbage hybrid in the 50-60 age, and China just utilizes self incompatible line preparation cabbage hybrid since the seventies.The deficiency of this method is: the parent must breed the cost height with artificial bud pollination method; Parent's inbreeding of more generation easily produces vitality and degenerates; Because self-incompatibillity is relative, therefore, the hybrid rate of the crossbreed seed that makes is difficult to reach 100%, can overcome above-mentioned shortcoming and utilize male sterile line to produce half-blood.Therefore, research and the available wild cabbage male sterile line of seed selection are domestic and international wild cabbage genetic breeding worker's research focuses always.Up to the present, the wild cabbage male sterility of research mainly is divided three classes both at home and abroad: the first kind is a recessive cytoblast sterile, Dickson (1970); Enge (1974); Goshew priorities such as (1974) is reported in the male sterile of finding to be subjected to recessive nuclear gene control in the brassica crop, such sterile material both can't find 100% sterile maintenance line, also be difficult to find the marker gene of interlocking, cost is too high again with tissue culture method breeding sterile material, thereby is difficult to utilize.Second class is the black mustard kytoplasm nucleo_cytoplasmic interaction wild cabbage male sterile of Perrason (1972) report, and this class sterile material exists petal not exclusively open, and nectary is undeveloped, can not attract major defects such as insect pollination.The 3rd class is the radish cytoplasmic sterility that derives from ogura, and the greatest problem of this sterile material is low temperature yellow in seedling stage, defective such as also exist flower not open in addition and nectary is undeveloped.In recent years, abroad some scholars utilize the method elimination Ougra radish dysgenic research of the allo-plasm aspect of cytomixis to make important progress.Basically, solved at present the problem of low temperature yellow in seedling stage.But from this domestic sterile material of new introducing, still exist nectary little on the new radish kytoplasm wild cabbage male sterile line, can not attract Pollinating Insect, defectives such as spontaneous pollination fecundity difference.Based on above various reasons, the wild cabbage male sterility breeding method fails to apply in the actual production of hybrid seeds so far always.
The objective of the invention is from wild cabbage natural population, to select the dominance genetic male sterile line to adopt the selfing/test cross/method of backcrossing to make this male sterile line become the stable dominance male sterile line that isozygotys, make this male sterile line sterile plant rate reach 100%, sterile degree reaches 100%, with good inbred line preparing hybrid kind, its hybrid rate reaches more than 95%.
The present invention is in long-term wild cabbage genetic breeding practice, from the natural population of wild cabbage starting material 79-399, find male sterile line plant 79-399-3, by the major gene resistance that studies have shown that its sterility is a pair of dominance nuclear gene control, on this basis, utilize the sensitivity characteristic of this material under certain genetic background and environmental condition, by selfing, backcross, method such as test cross, select the dominance male sterile line that isozygotys.The dominance of will isozygotying male sterile line and good temporary maintainer line hybridization obtain sterility and the stable male sterile line of economic characters, utilize the good hybridization between selfed lines of male sterile line and coordinate force again, make cabbage hybrid.Thereby founded the production of hybrid seeds new method of utilizing male sterile line preparation cabbage hybrid.
A kind of seed selection and producing method for seed thereof of wild cabbage dominance genetic male sterile line, comprise dominance caryogram male sterile gene selection, isozygoty, preserve, breeding male sterile lines, the preparing hybrid kind, the present invention selects dominant genic male sterility (containing the CDMs399-3 gene) from the natural population in wild cabbage material 79-399 breeding field, with the maintenance line outbreeding male sterile line of dominant genic male sterility and screening, with the inbred line preparing hybrid kind of dominant genic male sterility and breeding material.
With selfing, backcross, the test cross method is isozygotied dominant genic male sterility.
Utilize method for tissue culture, preserve the dominant genic male sterility that isozygotys.
Essence of the present invention is to utilize good dominance male sterile line to prepare the cabbage hybrid of strong advantage, has the following advantages:
1. the wild cabbage dominance male sterile line of seed selection of the present invention is better than all kinds wild cabbage male sterile material of up to the present having reported both at home and abroad:
1. sterile plant rate reaches 100%, significantly is better than sterile plant rate and has only 50% recessive sterile material.
2. sterility is controlled by the dominance nuclear gene, the harmful effect that the sterile cytoplasm of having avoided existing cytoplasmic sterility to exist brings.
3. main economic characters are good, and no low temperature yellow in seedling stage significantly is better than existing the R of low temperature yellow in seedling stage
1The kytoplasm wild cabbage is sterile.
It is normal to blossom and bear fruit, the nectary prosperity, and it is not exclusively open significantly to be better than petal, and nectary is little, can not attract the black mustard kytoplasm wild cabbage male sterile line of insect pollination and the new in recent years radish kytoplasm wild cabbage male sterile line of introducing through transforming well.
2. the male-sterile seed that is used for the production of hybrid seeds is formulated by the sterile clone of the dominance of isozygotying and temporary maintainer line open pollination in isolated area, vitality is strong, seed production height during the preparing hybrid kind, and without artificial pollination, more much lower than artificial bud pollination breeding self incompatible line cost.
3. sterility is stable.The hybrid rate of crossbreed seed can reach 100%.
Fig. 1 is male sterile line and breeding hybridized family tree
Fig. 2 is male sterile line hereditary pattern figure
Narrate embodiments of the invention below:
Content of the present invention comprises the discovery of wild cabbage dominance nuclear gene CDMs399-3; The genetic mechanism of sterility; Screening, the propagation technique of the dominance of isozygotying male sterile line; Male-sterile line breeding, propagation technique; Prepare half-blooded cross-breeding technology with male sterile line.One. the discovery of wild cabbage dominance nuclear gene CDMs399-3 and the genetic mechanism of sterility thereof
Spring in 1979 was found male sterile plant 79-399-3 from the natural population of wild cabbage starting material 79-399 multiplication fields.For studying the genetic mechanism of its sterility, do maternal with this sterile strain, educated strain with the 79-399-3 material is carried out test cross as male parent, 1981, observed 79-399-3 spring in 1982,1984,1991,1992,1993,1994 and the offspring that derives in isolated sterile strain and normal fertile strain test cross, its offspring's fertility is separation in 1: 1, and the self progeny of normal fertile strain can educate fully.The partial sterility strain trace-pollen occurs under certain genetic background and environmental condition, this sterile strain self progeny fertility with trace-pollen is separation (table 1, table 2) in 3: 1.
The test cross offspring fertility performance of sterile strain of table 1. and normal fertile strain
Time observation plant sum male sterile plants is counted fertile plant and is counted P (1: 1) 1,981 734 0.71 1,982 20 9 11 0.65 1,984 14 68 0.59 1,991 20 9 11 0.65 1,992 84 44 40 0.66 1,993 1,902 987 915 0.10 1,994 1,532 789 743 0.24 totals 3,558 1,836 1,720 0.25
Table 2. trace-pollen sensitive sterile strain self progeny fertility performance time observation plant sum male sterile plants is counted fertile plant and is counted P (3: 1) 1,993 147 103 44 0.17 1,994 314 227 87 0.27 totals 461 330 131 0.10
Above result shows: the sterility of this sterile material is subjected to a pair of dominance master to imitate the control of nuclear gene, and also carries sterile gene in the trace-pollen of the sterile strain of trace-pollen.Now will examine the dominance nuclear gene names and is CDMs399-3.Its hereditary pattern is as follows:
This sterile material leaf look green is or dark green, and no dead bud or dead bud were few after not yellow under the low temperature, sterile strain were buddingged, and flower opens, and the nectary prosperity can normally attract honeybee pollination.These proterties all significantly are better than black mustard and radish cytoplasmic sterility material.(table 3)
Several parts of wild cabbage male sterile materials of table 3. bloom plant and morphology of tea flower organ thereof
| Sterile type | Fertility | Plant height (cm) | 1 fraction number | The wax powder | The flowering state | Bud is long | Bud is wide | Petal is wide | Ovary is long | Stamen is long | Nectary |
| Dominance sterile material 92-438 | Sterile strain | 121.3 | 7.7 | In | Normally | 0.73 | 0.35 | 0.79 | 0.98 | 0.46 | Greatly |
| Can educate strain | 122.3 | 7.7 | In | Normally | 1.00 | 0.55 | 0.90 | 1.04 | 1.14 | Greatly | |
| Black mustard kytoplasm wild cabbage sterile material | Sterile strain | 108.3 | 14.7 | Many | Semi-open | 0.61 | 0.27 | 0.33 | 1.00 | 0.34 | Little or do not have |
| Can educate strain | 101.3 | 15.7 | In | Normally | 0.87 | 0.37 | 0.58 | 1.96 | 0.93 | Greatly | |
| Radish cytoplasmic sterility material | Sterile strain | 109.0 | 7.7 | Few | Semi-open | 0.74 | 0.42 | 0.75 | 0.78 | 0.35 | Little or do not have |
Result of study shows, after the principal character of sterile material flower pesticide abortion appears at tetraspore and forms soon.Fertile material is after tetraspore forms, and its callose decomposes, and microspore dissociates out, and corresponding sterile material callose does not decompose, and tetraspore continues to be wrapped in the callose wall and can not discharge and abortion gradually.
Result of study shows that also the different test cross offspring fertility performances of this sterile material can be divided into stablizes and two types of environment sensitives.Stability types sterile plant rate and sterile degree under the varying environment condition all can keep 100%, and the environment sensitive type is under certain genetic background and environmental condition, then micro-vital pollen (table 4) can appear, this situation explanation, also there is the influence of modifier gene in this material sterility except that being subjected to the control of dominance major gene resistance.
The fertility performance of the several sterile materials of table 4. under the varying environment condition
| Male sterile line | Sterile (%) | ||
| Booth | Open country | Cold bed | |
| 346A | 100 | 100 | 100 |
| 318P5 | 100 | 100 | 100 |
| 330L13 | 100 | 100 | 100 |
| 330P6 | 100 | 100 | 100 |
| 330P2 | 73.0 | 77.0 | 41.0 |
Annotate: sterile degree=(number is always spent in sterile flower sum ÷ investigation) * 100
Temperature is the main environment factor of the responsive type Fertility Expression of influence.Temperature (mainly being mean temperature and minimum temperature) reaches utmost point significant correlation level to the influence of Fertility Expression.In the 1-3 week of responsive to temperature period before blooming, before blooming 16-21 days sensitive periods,, critical mean temperature is about 15-20 ℃.
Test has proved that this dominance sterile material exists environmental sensitivity, and promptly the sterile plant that is under the heterozygous state at some dominant gene under certain genetic background and the environmental condition can have blodynamic trace-pollen.Among the self progeny of this trace-pollen sterile plant, separablely go out the sterile strain of the dominance of isozygotying that sterile gene isozygotys.
Sterile strain of the dominance of isozygotying and the sterile strain of heterozygosis dominance are difficult to distinguish in the sterility performance sometimes, for identifying the sterile strain of the dominance of isozygotying, the sterile strain that occurs among the sterile strain self progeny of all trace-pollens and 2-3 can be able to be educated the strain test cross, if it is 100% sterile that several test cross offsprings all show as, can think that then the maternal sterile strain of this test cross combination is the sterile strain of the dominance of isozygotying.The sterile strain of the good dominance of isozygotying that in breeding, utilizes, need not only that dominant sterile gene isozygotys, sterility is stable, and require its economic characters good, main proterties is neat, stable, therefore, when identifying whether sterile gene isozygotys, should identify its main economic characters in the field.
For selecting the good dominance male sterile line that isozygotys, generally adopt following method:
1. continuous selfing method: in sterile material colony, select the sterile plant selfing of the good fertility sensitivity of economic characters, and in its self progeny, continue to select the good and fertility sensitivity of proterties sterilely involve continuous selfing in a criminal case, through among the self progeny of 3-5 for purifying, can select good and the good dominance male sterile line that isozygotys that sterile gene isozygotys of economic characters.
2. second cycle line method (crossing inbred method): in sterile material colony, select the sterile strain and a good hybridization between selfed lines of the better sterility sensitivity of economic characters, sterile the involving in a criminal case about 5 generations of continuous selfing of in its offspring, selecting economic characters excellent sterile sensitivity then, can therefrom select the good sterile strain of the dominance of isozygotying.
3. backcross method: select economic characters better, the sterile strain of sterility sensitivity is maternal, and the selfing that economic characters are good, coordinate force is good is a male parent, and continuous backcross selects sensitive sterile strain selfing about 5 generations then, can therefrom select the good sterile strain of the dominance of isozygotying.
By evaluation in recent years, several parts of good isozygoty (tables 6) such as dominance male sterile line, 318P5,330L13,323P2,323P6 have been filtered out.
Several parts of good dominance male sterile line sterility of isozygotying of table 5. show nineteen ninety-five in time male sterile line test site in 1996 the booth open country total booth open country cold bed total of isozygotying
The several 37 20 12 23 16 51 318-P5 sterile plant rates 100 100 100 100 100 100 100 of investigation strain
Sterile degree 100 100 100 100 100 100 100
The several 336434 11 330-L13 sterile plant rates 100 100 100 100 100 100 100 of investigation strain
Sterile degree 100 100 100 100 100 100 100
The several 33333 9323-P2 sterile plant rates 100 100 100 100 100 100 of investigation strain
Sterile degree 100 100 100 100 100 100
The several 33 16 23 9 48323-P6 sterile plant rates 100 100 100 100 100 100 of investigation strain
Sterile degree 100 100 100 100 100 100
The good purifying dominance of table 6 male sterile line 318p5 sterility showed for 1996 spring
Investigation date place strain numbers 4.11 4.14 4.16 4.19 4.23 4.25 4.28 5.1 5.4 5.7 5.11
1----------------------big by 2----------------------canopy 3-----------------------
4----------------------Yang 5------------------furrow 6-------------------
7------------------Lu 8----------------ground 9-----------------
10----------------annotate:---expression is thoroughly sterile, the propagation technique of WUHUAFEN three, good dominance male sterile line
The sterile strain sterility of the dominance of isozygotying is stable, does not occur pollen under various varying environments, and therefore, the sterile strain of the dominance of isozygotying can not self propagated, need the method with tissue culture preserve, expand numerous under laboratory's condition.Generally carry out tissue culture spray of getting the sterile strain of dominance of isozygotying 4-5 month or lateral bud in laboratory, the tissue cultivating seedling that 9-10 month will take root is transplanted in the land for growing field crops.Winter, bloomed 1 year spring with protecting in the vernalization of surviving the winter.
If directly with isozygotying dominance male sterile line preparing hybrid kind then cost is too high, with dominance male sterile line and inbred line (maintenance line) hybridization that can educate of isozygotying, obtain the stable dominance male sterile line of sterility, the male sterile line production of hybrid seeds with this dominant sterile gene heterozygosis then can reduce production costs greatly.But, because the sterility of this sterile material is except that being subjected to the control of dominance major gene resistance, also be subjected to the influence of little effect modifier gene, the performance of sterility and the relation of genetic background are very close, therefore, not all inbred line of educating all can obtain the stable male sterile line of sterility with the dominance male sterile line hybridization of isozygotying, and needs by extensive testcross and screening temporary maintainer line, and good temporary maintainer line should have following condition:
With its offspring's sterility of dominance male sterile line test cross of isozygotying stable (sterile plant rate reaches 100%, throughout the year sterile degree 100% or be not less than 95%).
2. good, the stable and consistent of Other Main Agronomic Characters, and similar to the dominance male sterile line that isozygotys.
3. a little less than self-compatible or the incompatibility, the proterties of blossoming and bearing fruit is good.When isozygotying the dominance male sterile line with continuous selfing method or the seed selection of second cycle line method, temporary maintainer line is mainly by screening in the sisters system that can educate.When isozygotying the dominance male sterile line with the backcross method seed selection, the male parent of backcrossing itself just may be good temporary maintainer line
Do maternal with the good dominance male sterile line that isozygotys, temporary maintainer line is made male parent, both were colonizated in open pollination in the strict especially isolated area by 3: 1, promptly were to be used to prepare half-blooded dominance male sterile line seed by the seed of receiving on the dominance male sterile line plant of isozygotying.By screening in recent years, obtain 95-414,95-415,95-438,95-422,95-423,94-346,96-291,96-296, several parts of dominance male sterile lines such as 96-304,96-305 now, they are under the varying environment condition, sterile plant rate all reaches 100%, and sterile degree also reaches 100% or near 100% (table 7, table 8).
Several parts of good dominance male sterile line sterility performances of table 7.
1996 nineteen ninety-fives of dominance time
Educate is that test site booth open country adds up to booth open country cold bed to add up to ÷
Investigation strain several 10 35 45 3 81 8 92
94-346 sterile plant rate 100 100 100 100 100 100 100
Sterile degree 100 100 100 100 100 100 100
Investigation strain several 6 14 20 9 11 18 38
95-414 sterile plant rate 100 100 100 100 100 100 100
Sterile degree 100 100 100 99.9 100 99.9 99.9
Investigation strain several 64 10 439 16
95-415 sterile plant rate 100 100 100 100 100 100 100
Sterile degree 100 100 100 100 100 100 100
Investigation strain several 5388 32 24 64
95-438 sterile plant rate 100 100 100 100 100 100 100
Sterile degree 100 100 100 100 100 100 100
Table 8 dominance male sterile line 95-415 sterility showed for 1996 spring
Investigation date place strain numbers 4.11 4.14 4.19 4.23 4.25 4.28 5.1 5.4 5.7 5.11
1--------------------big by 2------------------canopy 3--------------------
4 --- --- --- --- --- --- -- --- ---
5 --- --- --- --- --- --- ---
6 --- --- --- --- --- --- ---
7----------------Yang 8--------------furrow 9-----------------
10 --- --- --- --- --- --- ---
11 --- --- --- --- --- --- ---
12 -- --- --- --- --- --- --- ---
13--------------Lu 14------------ground 15-------------
16 -- -- --- --- --- --- ---
Annotate:---expression is thoroughly sterile, WUHUAFEN four, the apolegamy of good hybrid combination and the apolegamy of the good hybrid combination of seeding technique () thereof
The method of the good hybrid combination of apolegamy wild cabbage dominance male sterile line is identical with other conventional breeding approach basically, select for use good, main proterties of coordinate force and source and male sterile line to have the inbred line of certain difference to do male parent and male sterile line hybridization, carry out combining ability test, select optimum combination.Be used as the inbred line self compatible line that preferably open pollination is bred under the isolation condition of male parent for the cost that reduces the male parent breeding.
By effort in recent years, tentatively select several fine combination such as 193,96 autumns 219 of 172,96 spring, 129,96 autumns, 128,96 spring, 118,96 spring, 96 spring, its main proterties sees Table 9.
The good hybrid combination that the several dominance male sterile lines of table 9. are allotted
Survey item
× ％ ( cm ) ( cm ) ( cm ) ( Kg ) ( Kg ) 96118 43.2×43.8 12.2 14.6×15.4 8.3 0.50 0.86 3931.1 38.71 5296128 34.6×34.8 9.0 11.8×13.7 6.9 0.68 0.69 3154.0 11.69 5296129 41.6×41.6 11.0 12.7×13.7 6.7 0.61 0.70 3199.7 12.90 5211 41.0×41.0 16.6 12.5×13.5 8.6 0.56 0.62 2834.0 CK 5296172 54.6×57.0 14.0 18.9×14.9 6.8 0.52 1.44 4113.5 6.1 5996193 58.0×55.4 17.2 21.2×14.5 7.6 0.42 1.44 4113.5 6.1 5996219 53.2×53.0 16.7 19.8×15.2 6.3 0.47 1.48 4227.8 9.1 598 59.6×60.6 16.2 25.2×12.7 5.2 0.40 1.67 3876.1 CK 82 ( )
1. proper seeding: wild cabbage is a plant vernalization plant, and male sterile line and male parent inbred line can be difficult for bolting because of plant is too small and bloom if sow evening, if sow disease take place easily not only too early, and in kind of a strain deuterogenesis early ageing.In the sowing time that the North China is suitable, precocious material is late August, late-maturing material late July to early August.
2. protection production of hybrid seeds parent's safe overwintering and finish vernalization: the middle part, North China and to the north of the area, plant strain and should heel under protective conditions such as cold bed and survive the winter.The south, North China is planted strain and can be survived the winter at open country, for frost damage prevention can be in kind of a strain base portion ridging.
3. the strict distance isolation of noting seed farm: wild cabbage is hybridized with brassica crop easily, therefore, wild cabbage seed farm and other wild cabbage class seed collecting fields sky need between isolation more than 1000 meters.
4. guarantee the honeybee source: wild cabbage is an entomophilous flower, and the male sterile line WUHUAFEN can not selfing, the full pollen pollination that provides by male parent inbred line during hybridization, and every mu of seed farm will be put a hive bee at least, could satisfy the needs of pollinating basically.
5. the suitable proportion when controlling parents' production of hybrid seeds: use the male sterile line production of hybrid seeds, having only the seed of gathering in the crops from the male sterile line plant is half-blood, for improving the output of crossbreed, can suitably strengthen the ratio of male sterile line kind strain during the production of hybrid seeds, the ratio of general male sterile line was advisable with 3: 1.
6. strict guarantee parental purity: in each link of the production of hybrid seeds, note parent's roguing, flowering stage if accidental trace-pollen strain now in male sterile line also should try one's best and pull out as early as possible.
Claims (3)
1, a kind of seed selection and producing method for seed thereof of wild cabbage dominance genetic male sterile line, comprise dominance caryogram male sterile gene selection, isozygoty, preserve, breeding male sterile lines, the preparing hybrid kind, it is characterized in that: from the natural population in wild cabbage material 79-399 breeding field, select dominant genic male sterility (containing the CDMs399-3 gene), with the maintenance line outbreeding male sterile line of dominant genic male sterility and screening, with the inbred line preparing hybrid kind of dominant genic male sterility and breeding material.
2, method according to claim 1 is characterized in that: adopt selfing, backcross, the test cross method is isozygotied dominant genic male sterility.
3, method according to claim 1 is characterized in that: utilize method for tissue culture to preserve dominant genic male sterility.
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| CN102349439A (en) * | 2011-08-25 | 2012-02-15 | 温州科技职业学院 | Breeding and seed producing method for recessive gene male sterile line of cauliflower |
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| CN109554497A (en) * | 2018-12-20 | 2019-04-02 | 中国农业科学院蔬菜花卉研究所 | PCR primer, kit and its application for the screening of wild cabbage Recessive male sterile gene |
| CN109988862B (en) * | 2019-04-24 | 2021-07-02 | 中国农业科学院蔬菜花卉研究所 | PCR (polymerase chain reaction) marker related to male sterility of dominant nuclear gene of cabbage and application of PCR marker |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1044380A (en) * | 1989-01-27 | 1990-08-08 | 沈阳市农业科学研究所 | The breeding of Chinese Cabbage through genes of cell nucleus interacting and malesterile line technology |
| CN1047186A (en) * | 1990-07-11 | 1990-11-28 | 内蒙古赤峰市农业科学研究所 | The selectively breeding technique of male sterile system with mutual action of dorninant genes in nucleus of millet |
| CN1054700A (en) * | 1990-03-07 | 1991-09-25 | 山西省农业科学院蔬菜研究所 | Technology for breeding high-yield and quality radish by hybridisation |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1044380A (en) * | 1989-01-27 | 1990-08-08 | 沈阳市农业科学研究所 | The breeding of Chinese Cabbage through genes of cell nucleus interacting and malesterile line technology |
| CN1054700A (en) * | 1990-03-07 | 1991-09-25 | 山西省农业科学院蔬菜研究所 | Technology for breeding high-yield and quality radish by hybridisation |
| CN1047186A (en) * | 1990-07-11 | 1990-11-28 | 内蒙古赤峰市农业科学研究所 | The selectively breeding technique of male sterile system with mutual action of dorninant genes in nucleus of millet |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102349439A (en) * | 2011-08-25 | 2012-02-15 | 温州科技职业学院 | Breeding and seed producing method for recessive gene male sterile line of cauliflower |
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