CN102349439A - Breeding and seed producing method for recessive gene male sterile line of cauliflower - Google Patents
Breeding and seed producing method for recessive gene male sterile line of cauliflower Download PDFInfo
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- CN102349439A CN102349439A CN2011102463818A CN201110246381A CN102349439A CN 102349439 A CN102349439 A CN 102349439A CN 2011102463818 A CN2011102463818 A CN 2011102463818A CN 201110246381 A CN201110246381 A CN 201110246381A CN 102349439 A CN102349439 A CN 102349439A
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- 240000003259 Brassica oleracea var. botrytis Species 0.000 title claims abstract description 36
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- 238000009395 breeding Methods 0.000 title abstract description 9
- 230000001488 breeding effect Effects 0.000 title abstract description 9
- 108700005079 Recessive Genes Proteins 0.000 title abstract description 7
- 102000052708 Recessive Genes Human genes 0.000 title abstract description 7
- 230000010152 pollination Effects 0.000 claims abstract description 8
- 230000036512 infertility Effects 0.000 claims abstract description 7
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- 238000002955 isolation Methods 0.000 claims abstract description 3
- 241000196324 Embryophyta Species 0.000 claims description 7
- 230000035558 fertility Effects 0.000 claims description 7
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- 208000007466 Male Infertility Diseases 0.000 description 3
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a breeding and seed producing method for a recessive gene male sterile line of cauliflower. The invention adopts the technical scheme that a recessive gene male sterile mutant strain of the cauliflower is subjected to sister hybrid and progeny selfing separation to breed a sterile strain with the sterile rate of 100 percent and the sterile degree reaching or approximating to 100 percent; the sterile line used as a female parent is hybridized and combined with an excellent selfing line; and a combined hybrid seed is produced by applying a honeybee pollination method. The invention has the advantages that: firstly, a new sterile source for heterosis advantage utilization of the cauliflower is provided and the defects of cytoplasmic sterility are overcome; secondly, the recessive gene male sterile line is maintained and reproduced by using a tissue culture technology and various works for filed isolation and preproduction of the sterile line and a maintenance line are omitted, so that the influence on preproduction of the sterile line caused by weather, season, land and isolating conditions is avoided; and thirdly, a hybrid strain is produced by using the recessive gene male sterile line and honey can be used for pollination, and thus the seed production cost is reduced and the seed production efficiency is improved.
Description
Technical field
The present invention relates to the method that a kind of vegetable heterosis is utilized, be meant the seed selection of cauliflower recessive karyon male sterile line and the method for the production of hybrid seeds especially.
Background technology
Cauliflower is the Cruciferae Brassica plants, is the important vegetable crop of China, has tangible hybrid vigour, and the half-blood advantage is extensive use on producing.Produce cauliflower half-blood seed and mainly contain three kinds of approach.The one, adopt the hybridization of artificial stripping flower bud, the 2nd, utilize self-incompatibillity, the 3rd, utilize cytoplasmic male sterility.Preceding two kinds of methods are extensive use on producing, but first method is time-consuming, and breeding cost is high, and hybrid rate does not reach 100%, the seed quality instability; Self incompatible line parent propagation difficulty is big, and parent's inbreeding of more generation is prone to degenerate.Utilize male sterile line preparation half-blood, can overcome the shortcoming of above two kinds of approach, breeding cost is low, and seed purity is high, has become a main trend of cauliflower breeding.But China's present cauliflower male sterile research is more is that the Ogura radish cytoplasmic sterility source transformation that will abroad introduce is in cauliflower; Produce cytoplasmic sterile line; But this sterile source exist nectary little, can not the normal suction insect pollination and shortcoming such as abnormal flower, limited its large-area applications on producing.The present available cauliflower male sterile line of China is also fewer, and main source Ogura sterile source, and kytoplasm is single, and blood relationship is nearer, and the crossbreed hereditary basis that possibly cause disposing is narrow, causes the resistance to environment to descend.
Summary of the invention
Summary of the invention
The objective of the invention is to overcome the deficiency that prior art exists; A kind of selection of recessive karyon male sterile line is provided; Breed a kind of recessive karyon male sterile line; For the cauliflower heterosis utilization provides new sterile source; Enrich the type in cauliflower male sterile source, can avoid the too single adverse effect that causes of sterile type; Utilize the recessive karyon male sterile line to prepare good hybrid combination, set up and utilize the recessive karyon male sterile line to carry out the new method of hybrid seeding, reduce breeding cost, improve seed production efficiency.
For realizing above-mentioned purpose; The technical scheme of selection of the present invention is: a kind of selection of cauliflower recessive karyon male sterile line; It is characterized in that: the male sterility mutant strain of cauliflower is used the pollen of its sisters' strain be its pollination; Obtain sisters and hand over seed; Hand over the cauliflower of cultivating seeds to carry out selfing with sisters then; Fertility takes place and separates in the offspring; The sterile strain of trace-pollen appears in the generation flower; Selfing is carried out in the sterile strain that trace-pollen is appearred in flower more again; Isolate complete sterile strain; Get complete sterile strain and carry out tissue culture propagation; Keep its sterility; Through observation and screening, therefrom select a sterile plant rate and reach 100%, sterile degree reaches or near 100% sterile line.
The technical scheme of producing method for seed of the present invention is: sterile strain rhachis and the holder of getting cauliflower recessive karyon male sterile line the annual 3-4 month carry out tissue culture and expand numerous male sterile line; 8-9 month rooting and transplant is promptly maternal; Early October is that male parent is colonizated in the same isolation canopy with corresponding cauliflower inbred line, and this ratio of father and mother is 1:2 or 1:3; Early flowering season begin to utilize honeybee pollinate the preparation crossbreed.
The cauliflower recessive karyon male sterile line plant type of breeding through such scheme is compact, and the leaf look grayish green, and the wax powder is medium, and to gathering about 50 days, the bouquet consolidation is pure white from field planting, internal lobe button bag bouquet.Flowering is normal, and corolla is faint yellow, and contabescence is at base portion, and length is less than 1/2 of gynoecium, flower pesticide halberd shape; Column cap is big, and column cap obviously exposes when blooming, and mastoid process is obvious, grows normally, and nectary is flourishing, the honeybee pollination of ability normal suction, and the solid ability of honeybee pollination is strong.We are numbered 9901A with this male sterile line.
The fertility performance of cauliflower recessive karyon male sterile line 9901A:
Cauliflower recessive karyon male sterile line 9901A all can educate with the F1 that different cauliflower hybridizations between selfed lines obtain, and can infer that this sterile material sterility controlled by recessive gene, can educate to sterile be dominance.F2 separates for fertility, shows as to educate: sterile segregation ratio infers that near 3 : 1 its sterility might be to be controlled by single recessive gene.
In order further to confirm the fertility of cauliflower recessive karyon male sterile line, we observe cauliflower recessive karyon male sterile line sterile plant rate and sterile degree in different year, have also carried out the bagging selfing simultaneously, and the result sees table 1, and 2.The result shows that this male sterile line sterile plant rate reaches 100%, and sterile degree reaches or near 100%.
The fertility performance of table 1 9901A
Be further described in the face of the present invention down.
Embodiment
Using the pollen of its sisters' strain with the male sterility mutant strain of cauliflower is its pollination; Obtain sisters and hand over seed; Hand over the cauliflower of cultivating seeds to carry out selfing with sisters then; Fertility takes place and separates in the offspring; The sterile strain of trace-pollen appears in the generation flower; Selfing is carried out in the sterile strain that trace-pollen is appearred in flower more again; Isolate complete sterile strain; Get complete sterile strain and carry out tissue culture propagation; Keep its sterility; Through observing for many years and screening, therefrom select a sterile plant rate and reach 100%, sterile degree reaches or near 100% sterile line (we are the cauliflower recessive karyon male sterile line called after 9901A of institute's seed selection).With 9901A is maternal; (male parent 9908 is inbred lines that Wenzhou landrace roundleaf was bred through continuous 5 generations selfing purifying in 70 days to cauliflower inbred line 9908 for male parent; About 70 days breeding times; Resistance, the bouquet consolidation is pure white, the flower bud grain is fine and closely woven, internal lobe button bag bouquet); The hybrid combination bowl snow that makes 60 days shows good.
Produce the method for cauliflower crossbreed with the recessive karyon male sterile line: 1, the breeding of recessive karyon male sterile line: utilize plant tissue culture technique propagated cell genic male sterile line.Get in general annual 3-4 month sterile strain rhachis or holder carry out tissue culture expand numerous, 8-9 month rooting and transplant.Explant is in inducing culture MS+BA 2 mg/l+2; 4-D 0.01 mg/l inducing culture 20 days; Enrichment culture is 25 days on proliferated culture medium MS+BA 0.1-0.2 mg/l+Kt 0.1-0.2 mg/l+NAA 0.001-0.003mg/l; Growth coefficient can reach 5-6 doubly; Propagation expands numerous continuously; Forward root media 1/2MS+NAA 0.1-0.3 mg/l culture of rootage August to about l5 days, rooting rate can reach more than 98%.Test-tube plantlet uncork after taking root refining seedling 2-3 days is transplanted in pearlite interstitial substance or the clean river sand, transition is transplanted to big field after cultivating for one to two week in the full exposure auto spraying facility.2, the production of hybrid seeds (1) field planting is in good time begun sowing in good time and field planting according to father and mother flowering stage originally, makes father and mother's flower synchronization originally as far as possible, is to prolong the male parent florescence, and male parent can be divided the field planting of two phases, and two period interval times were 1-2 week.Maternal tissue cultivating seedling can be transplanted to the field at the beginning of 9 months by the end of August, 10 field planting just, and male parent 9908 can be sowed at the beginning of 9 months by the end of August, divided the field planting of two phases at the beginning of 10 months by the end of September, and this ratio of father and mother is 1:2 or 1:3.(2) rational close planting: the seed farm planting density is advisable about with every mu 3500 strain.(3) strict roguing, in each link of the production of hybrid seeds, assorted strain and the sick and weak strain that does not meet parent typical case proterties pulled out in strictness, guarantees seed purity and seed production quality.(4) cut in good time ball, dredge branch and draw tongue, remove Lao Ye.Bouquet selects fine day to cut bouquet near the commodity bouquet results stage, cuts before the ball and does not water in 4-5 days, is coated with carbendazim powder and topsin powder in incision immediately after cutting; Remove base portion Lao Ye when cutting ball simultaneously, reservation function leaf 10-12 opens, and is beneficial to ventilation and penetrating light, prevents that the humidity height causes rotten in the canopy.Add forced ventilation after cutting ball, reduce humidity in the canopy, promote wound healing.Spray is long during to the 20cm left and right sides, suitably beats tongue according to parents' florescence, dredge the sick and weak thin weak point of a part, bad, the cauline leaf midaxilla generation side shoot of flower bud development, and 5-6 healthy and strong spray stayed in general every strain, and built is fixed.Tangent plane will tilt when cutting spray, in time smears powder of carbendazim after cutting in case wound ponding rots.(5) pollinate and gather.Early flowering season begins each booth and puts a little case honeybee and pollinate.After pollination finishes, in time extract whole buds in maternal later stage, and the strain of male parent kind is pulled out totally, guarantee seed purity, guarantee that seed is fully ripe.When the flavescence of maternal strain kind pod, during seed maturity, gather the threshing in time and the storage of basking seeds in batches.
Claims (2)
1. the selection of a cauliflower recessive karyon male sterile line; It is characterized in that: the male sterile mutant strain of cauliflower is used the pollen of its sisters' strain be its pollination; Obtain sisters and hand over seed; Hand over the cauliflower of cultivating seeds to carry out selfing with sisters then; Fertility takes place and separates in the offspring; The sterile strain of trace-pollen appears in the generation flower; Selfing is carried out in the sterile strain that trace-pollen is appearred in flower more again; Isolate complete sterile strain; Get complete sterile strain and carry out tissue culture propagation, keep its sterility, through observing and screening; Therefrom select a sterile plant rate and reach 100%, sterile degree reaches or near 100% male sterile line.
2. according to the producing method for seed of the cauliflower recessive karyon male sterile line of the described selection gained of claim 1; It is characterized in that: sterile strain rhachis and the holder of getting cauliflower recessive karyon male sterile line the annual 3-4 month carry out tissue culture and expand numerous male sterile line; 8-9 month rooting and transplant is promptly maternal; Early October is that male parent is colonizated in the same isolation canopy with corresponding cauliflower inbred line, and this ratio of father and mother is 1:2 or 1:3; Early flowering season begin to utilize honeybee pollinate the preparation crossbreed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011102463818A CN102349439A (en) | 2011-08-25 | 2011-08-25 | Breeding and seed producing method for recessive gene male sterile line of cauliflower |
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| CN2011102463818A CN102349439A (en) | 2011-08-25 | 2011-08-25 | Breeding and seed producing method for recessive gene male sterile line of cauliflower |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168681A (en) * | 2013-04-16 | 2013-06-26 | 镇江瑞繁农艺有限公司 | Breeding method of early-ripe and disease-resistant broccolini |
| CN103262794A (en) * | 2013-05-08 | 2013-08-28 | 镇江瑞繁农艺有限公司 | Method for rapid propagation of cauliflower cytoplasm male sterility line |
| CN103270944A (en) * | 2013-06-20 | 2013-09-04 | 四川省农业科学院水稻高粱研究所 | Rapid propagation method of radish breeding material |
| CN108901833A (en) * | 2018-09-07 | 2018-11-30 | 重庆市农业科学院 | A method of branched out using axillary bud and improves early maturing cabbage first-filial generation hybrid seed yield |
| CN110301349A (en) * | 2019-08-12 | 2019-10-08 | 浙江省农业科学院 | A kind of selection of green pagoda cauliflower cenospecies |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072889C (en) * | 1996-11-28 | 2001-10-17 | 中国农业科学院蔬菜花卉研究所 | Tech. For breeding and prodn. of hybrid seeds of wild cabbage dominance male sterile line |
| CN101263784A (en) * | 2008-04-30 | 2008-09-17 | 贵州省生物技术研究所 | Rapeseed cultivar stem segment tissue culture fast breeding male sterile lines seed making method |
-
2011
- 2011-08-25 CN CN2011102463818A patent/CN102349439A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072889C (en) * | 1996-11-28 | 2001-10-17 | 中国农业科学院蔬菜花卉研究所 | Tech. For breeding and prodn. of hybrid seeds of wild cabbage dominance male sterile line |
| CN101263784A (en) * | 2008-04-30 | 2008-09-17 | 贵州省生物技术研究所 | Rapeseed cultivar stem segment tissue culture fast breeding male sterile lines seed making method |
Non-Patent Citations (2)
| Title |
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| 《上海蔬菜》 20051231 邵泰良等 熊蜂在花椰菜杂交制种中的应用效果 , 第2期 * |
| 《青海农林科技》 19901231 高京草等 花椰菜组织培养研究 , 第3期 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168681A (en) * | 2013-04-16 | 2013-06-26 | 镇江瑞繁农艺有限公司 | Breeding method of early-ripe and disease-resistant broccolini |
| CN103262794A (en) * | 2013-05-08 | 2013-08-28 | 镇江瑞繁农艺有限公司 | Method for rapid propagation of cauliflower cytoplasm male sterility line |
| CN103270944A (en) * | 2013-06-20 | 2013-09-04 | 四川省农业科学院水稻高粱研究所 | Rapid propagation method of radish breeding material |
| CN103270944B (en) * | 2013-06-20 | 2015-09-02 | 四川省农业科学院水稻高粱研究所 | A kind of quick-breeding method of radish breeding material |
| CN108901833A (en) * | 2018-09-07 | 2018-11-30 | 重庆市农业科学院 | A method of branched out using axillary bud and improves early maturing cabbage first-filial generation hybrid seed yield |
| CN110301349A (en) * | 2019-08-12 | 2019-10-08 | 浙江省农业科学院 | A kind of selection of green pagoda cauliflower cenospecies |
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Application publication date: 20120215 |