CN103168681A - Breeding method of early-ripe and disease-resistant broccolini - Google Patents
Breeding method of early-ripe and disease-resistant broccolini Download PDFInfo
- Publication number
- CN103168681A CN103168681A CN2013101315517A CN201310131551A CN103168681A CN 103168681 A CN103168681 A CN 103168681A CN 2013101315517 A CN2013101315517 A CN 2013101315517A CN 201310131551 A CN201310131551 A CN 201310131551A CN 103168681 A CN103168681 A CN 103168681A
- Authority
- CN
- China
- Prior art keywords
- sedge
- breeding method
- blue
- male
- disease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000009395 breeding Methods 0.000 title claims abstract description 23
- 201000010099 disease Diseases 0.000 title claims description 16
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims description 16
- 230000001086 cytosolic effect Effects 0.000 claims abstract description 17
- 230000008774 maternal effect Effects 0.000 claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 claims abstract description 14
- 206010021929 Infertility male Diseases 0.000 claims abstract description 13
- 208000007466 Male Infertility Diseases 0.000 claims abstract description 13
- 230000009466 transformation Effects 0.000 claims abstract description 12
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims abstract description 10
- 241001290610 Abildgaardia Species 0.000 claims description 35
- 235000017647 Brassica oleracea var italica Nutrition 0.000 claims description 29
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 claims description 28
- 241000196324 Embryophyta Species 0.000 claims description 24
- 238000009331 sowing Methods 0.000 claims description 16
- 240000007124 Brassica oleracea Species 0.000 claims description 12
- 238000009396 hybridization Methods 0.000 claims description 10
- 230000010152 pollination Effects 0.000 claims description 8
- 241000256844 Apis mellifera Species 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 7
- 210000000805 cytoplasm Anatomy 0.000 claims description 6
- 240000003259 Brassica oleracea var. botrytis Species 0.000 claims 6
- 208000000509 infertility Diseases 0.000 abstract description 8
- 230000036512 infertility Effects 0.000 abstract description 8
- 208000021267 infertility disease Diseases 0.000 abstract description 8
- 208000035240 Disease Resistance Diseases 0.000 abstract description 7
- 239000000463 material Substances 0.000 abstract description 7
- 230000008901 benefit Effects 0.000 abstract description 5
- 238000003976 plant breeding Methods 0.000 abstract 1
- 244000308180 Brassica oleracea var. italica Species 0.000 description 23
- 230000001488 breeding effect Effects 0.000 description 5
- 235000009508 confectionery Nutrition 0.000 description 5
- 230000035558 fertility Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 238000010899 nucleation Methods 0.000 description 5
- 235000013311 vegetables Nutrition 0.000 description 5
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000002955 isolation Methods 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 230000001850 reproductive effect Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 235000005731 Bambusa membranacea Nutrition 0.000 description 2
- 241000722731 Carex Species 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- NNBFNNNWANBMTI-UHFFFAOYSA-M brilliant green Chemical compound OS([O-])(=O)=O.C1=CC(N(CC)CC)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](CC)CC)C=C1 NNBFNNNWANBMTI-UHFFFAOYSA-M 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 230000009931 harmful effect Effects 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- 235000013411 Alpinia speciosa Nutrition 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 244000207867 Pistia stratiotes Species 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a breeding method of crops, and particularly to a breeding method of broccolini, belonging to the technical field of plant breeding. The breeding method has the advantages that 1. the male sterility line belongs to cytoplasmic male sterility and maternal inheritance, and is stable in sterility, the sterile plant rate and the sterility are 100 percent, nectary is developed, more nectars grow, stigma is normal, flowering and fruiting characters are good, and a seedling does not yellow under the condition of low temperature and can not deteriorate after multi-generation backcross, transform is easy, growth is vigorous, and combining ability is strong; 2. a transformation early generation sterile line is stronger in growth vigor in comparison with a maintainer line and has the advantage of interspecies cross; 3. a later producing shoot of the transformation early generation sterile line is stronger than that of the maintainer line, and thus the transformation early generation sterile line material is early planted by 5 days in comparison with a backcross male parent; 4. F1 is prepared by using the sterile line, the seed yield is high, the seed producing cost is low and the labor is saved; and 5. a new variety prepared by using the sterile line is strong in disease resistance, and is remarkable in production increasing.
Description
Technical field
The present invention relates to the breeding method of a kind of crops, the breeding method of the blue a kind of sedge in especially a kind of west belongs to field of plant variety breeding technology.
Background technology
The blue a kind of sedge in west is a kind of novel vegetable that is formed by broccoli and cabbage mustard selection cross, claims again slight broccoli, blue and white bamboo shoot, asparagus broccoli, English Broccolini by name, another name Sweet Baby Brocclini.The blue a kind of sedge in west is mainly edible with the tender colored a kind of sedge of fertilizer, and flower a kind of sedge look green is emerald green beautiful, and meat is tender and crisp, and local flavor is fragrant and sweet, and is nutritious, is rich in vitamin A, B, C, E, protein, anthocyan, the multiple nutrients compositions such as mineral matter.It has gathered the advantage of broccoli and cabbage mustard, has removed again the shortcoming of broccoli and cabbage mustard simultaneously.It is sweet crisp better to eat than broccoli, and is more fragrant and more sweet than cabbage mustard, the puckery blue or green taste that does not but have cabbage mustard often to have; Beautiful, the local flavor of its outward appearance good far away on broccoli and cabbage mustard.In addition, western blue a kind of sedge also has stomach invigorating aid digestion and be rich in anthocyan and cancer-resisting substance, and cancer-resisting substance wherein exceeds several times than broccoli, is a kind of delicious food and high-quality, the high-grade functional form green vegetables of health care perfect adaptation.Be that " the blue and white bamboo shoot of gold " are present by laudatory title in Taiwan, the blue a kind of sedge in Vegetable Base positive establishing in large scale west is with the supply domestic and international market.The blue a kind of sedge in west has stronger hybrid vigour, and production first-filial generation mainly contains two kinds of approach at present, and namely utilizing self incompatible line and dominant genic male sterility is the preparing hybrid kind.But utilize the self incompatible line production of hybrid seeds will spend a large amount of manpowers and the time is carried out parent propagation (by artificial bud pollination), cost is very high, and popularizing area is restricted by production of hybrid seeds amount.More disadvantageously the parent is long-term degenerates from transaction generation vitality continuously, and some good proterties can be lost, and the hybrid rate of crossbreed is difficult to reach 100%.Utilizing dominant genic male sterility is that the production of hybrid seeds is cumbersome, and complexity is difficult for promoting, and utilizes the cytoplasmic male sterile line production of hybrid seeds can overcome a series of defectives of utilizing the production of hybrid seeds such as self incompatible line.although China to cytoplasmic male sterility research start late, in cytoplasmic male sterility research, the breeder of China has done further research on the basis of Introduced From Abroad achievement in research and material, make some progress, by the improvement broccoli cytoplasmic male sterility source of introducing, sterility is stable, be not subjected to environmental influence, development of floral organs is good, stamen is all degenerated, gynoecium is normal, nectary is large, nectar is many, sterile plant rate and sterile degree are 100%, overcome low temperature yellow in seedling stage, the development of floral organs anomaly, ripening rate is high, all cole vegetables kinds can be as its maintainer.The trophosome of the blue a kind of sedge results in west when utilizing cytoplasmic male sterile line preparing hybrid kind, does not need restorer, only needs male parent to be, existing cross combination which has strong superior is used on producing.
Summary of the invention
The objective of the invention is the defective for the prior art existence, propose the breeding method of a kind of precocity, the blue a kind of sedge in disease-resistant west, improve the quality of western blue a kind of sedge.
The present invention is the technical solution problem by the following technical programs: the breeding method of a kind of precocity, the blue a kind of sedge in disease-resistant west comprises the following steps:
Step 1, take the broccoli cytoplasm male sterility line as maternal, be male parent with the good and stable selfing of broccoli, obtain first-filial generation seed F1;
Step 2, with described first-filial generation seed F1 sowing, through field test, observe under plant low temperature whether yellow of seedling, whether the reproductive stage flower is opened normally, and whether stamen degenerates, whether gynoecium is normal, whether floral organ grows normally, the nectary size, and nectar is how much, and sterile plant rate and sterile degree whether 100%, select the good male sterile line individual plant of comprehensive agronomy proterties;
Step 3, the inbred line that described broccoli is good and stable are carried out 4-5 for backcross transformation as backcross male parent and described male sterile line individual plant, obtain cytoplasmic male sterile line, and the good and stable selfing of described broccoli is its maintainer;
Step 4, take described cytoplasmic male sterile line as maternal, be male parent with the good and stable selfing of cabbage mustard, hybridization obtains western blue a kind of sedge new varieties.
The described broccoli cytoplasm male sterility line code name CMS055 of said method, the inbred line code name 0315 that described broccoli is good and stable, described cytoplasmic male sterile line code name CM09, the inbred line code name 0532 that described cabbage mustard is good and stable.
In described step 1, during hybridization, male parent and female parent are manually shelled flower bud or florescence bagging hybridization, after exposing column cap, the pollen of male parent is coated on maternal column cap, column cap is normally expanded.
In described step 3, with the cultivation of 1:2 ratio, rain shelter cultivation is adopted in cultivation to male sterile line, keeps ground moistening with corresponding maintainer, and temperature ventilates when surpassing 30 ℃; Put honeybee pollination flowering stage in canopy, each booth 2 case, the florescence is pulled out maintainer later.
In described step 4, seed farm is taked quarantine measures, 〉=1500 meters of distances, and mid-September is selected in sowing.When described male parent is grown to 5-6 sheet true leaf with maternal seedling stage, in the field planting of 1:2 ratio, rain shelter cultivation keeps ground moistening, ventilates when temperature surpasses 30 ℃.During the production of hybrid seeds, the more maternal late sowing kind of male parent 5-6 days, take adjust described male parent with maternal florescence as consistent.Florescence puts honeybee pollination in canopy, pull out male parent after the florescence.
Described backcrossing manually shelled flower bud when referring to male sterile line and backcrossing the male parent flower synchronization or the florescence bagging backcrosses; As flowering asynchronism, can remove major branch, short its collateral development is regulated the florescence, manually shells flower bud or florescence bagging hybridization during flower synchronization.Transformation is generation early, the cytoplasmic male sterile line male parent sowing in 5 days in advance that backcrosses.
the present invention is normal by the good broccoli cytoplasmic male sterile line CMS055(pistil function of having collected, harmful effect without the allos kytoplasm, economical character is preferably arranged, and sterility is thoroughly stable, sterile plant rate and sterile degree all reach 100%, have higher output coordinate force) with the ripe property of the good and stable inbred line 0315(of broccoli early, plant type is compact, in bouquet, bouquet is more loose, in the flower bud grain, it is strong that lateral branching is sprouted ability, disease resistance is strong, strong stress resistance) first hybridization, after (pistil function is normal through the 5 generation good cytoplasmic male sterile line CM09 of Comprehensive Traits that form of backcross transformation, harmful effect without the allos kytoplasm, economical character is preferably arranged, sterility is thoroughly stable, sterile plant rate and sterile degree all reach 100%, has higher output coordinate force).The male parent that backcrosses 0315 is maintainer.In the good and stable ripe property of inbred line 0532(of cytoplasmic male sterile line CM09 and cabbage mustard, plant type is little, the leaf look green, in flower a kind of sedge, in main a kind of sedge rugosity, side a kind of sedge sprouts that ability is strong, lateral branching length 15～20cm, picking time reach 25 days, disease resistance, strong stress resistance, coordinate force is good, pollen amount is large) hybridization, through field test, screening, select precocity, disease-resistant good western blue a kind of sedge new varieties (No. 1, auspicious a kind of sedge).
Advantage of the present invention is as follows: (1) male sterile is cytoplasmic sterility, and with maternal inheritance, its sterility is stable, sterile plant rate and sterile degree are 100%, nectary is flourishing, and nectar is many, and column cap is normal, the proterties of blossoming and bearing fruit is good, not yellow under the seedling cryogenic conditions, many generations backcross does not degenerate, and transformation is easy, growth potential is prosperous, and combining ability is strong.(2) in transformation generation early,, male sterile line is stronger than maintainer plant strain growth gesture, has the interspecific cross advantage.(3) in transformation generation early,, the late bolting of male sterile line is stronger than maintainer, so transformation is early for sterile line material ratio male parent early sowing 5 days that backcross.(4) prepare F1 with male sterile line, seed production is high, and breeding cost is low, the saving of labor.(5) strong with male sterile line preparation new varieties disease resistance, volume increase obviously.Breeding method is easy, and is with low cost, is convenient to promote, and market prospects are wide.
Description of drawings
Fig. 1 is the breeding flow chart of one embodiment of the invention.
Embodiment
Embodiment
In the present embodiment for the first time as maternal good and stable inbred line standard code 0315, broccoli cytoplasm male sterility line standard code CM09, the good and stable inbred line standard code 0532 of broccoli of broccoli cytoplasm male sterility line standard code CMS055, broccoli, can buy on market as required and (for example buy to Ruifan Agricultural Gardening Co., Ltd., Zhenjiang City, No. 112, Ningbo-Hangzhou road, Jurong City, Jiangsu Province, address), the present invention is practical.
As shown in Figure 1, the breeding flow process of the present embodiment is as follows:
First Year is from Europe, many parts of broccoli male sterile materials are introduced on the ground such as Japan, early September in the same year is in the Vegetable Base plantation of the Zhenjiang institute of agricultural sciences, through field observation, identify, find 1 part of material (CMS055) not yellow of seedling at low temperatures, the reproductive stage flowering is normal, stamen is all degenerated, gynoecium is normal, nectary is large, nectar is many, the fine individual plant of selecting and remain after field test is heeled in booth and survives the winter, Second Year early April is with 7 parts of dissimilar (different maturation period, different leaf and leaf looks) the good and stable inbred line (material) of broccoli is hybridized with it, obtain 7 parts of dissimilar hybridization F1 offsprings, results F1 seed at the beginning of 6 months then, September, F1 was not for waiting owing to gathering in the crops seed amount with 7 parts of dissimilar F1 and the male parent that backcrosses sowing then, and several 50～70 strains of plant do not wait.repeatedly identify each plant growing way in process of growth, disease resistance, resistance and other comprehensive agronomy proterties, check the fertility of F1 plant at duration of flowering, screening sterile plant rate and flower sterile degree are 100% sterile strain, the wherein sterile strain not yellow of seedling at low temperatures of 1 part of material (male parent is 0315), the reproductive stage flowering is normal, stamen is all degenerated, gynoecium is normal, nectary is large, nectar is many, Comprehensive Traits is good, then the sowing for many years in batches of male parent 0315 seed backcrosses F1 and the male parent 0315(that backcrosses) manually shell flower bud during flower synchronization or the florescence bagging backcrosses, in early June, the 3rd obtains the BC1 seed, to the BC1 that obtains and the male parent 0315 that backcrosses in early September sowing then, plantation 71 strains.Choose 15～20 individual plant baggings, in early April, the 4th finds that in field examination BC1 fertility the stamen of BC1 is all degenerated, gynoecium is normal, and nectary is large, and nectar is many, sterile rate and sterile degree are still 100%, recycle the male parent 0315 that backcrosses and backcross, and in early June, the 4th obtains backcross progeny BC2 seed; Early September is to the BC2 that obtains and male parent 0315 sowing that backcrosses, plantation 50 strains then.Choose 10～15 individual plant baggings, in early April, the 5th field examination BC2 fertility, discovery BC2 stamen is all degenerated, gynoecium is normal, and nectary is large, and nectar is many, sterile rate and sterile degree are still 100%, recycle the male parent 0315 that backcrosses and backcross, and in early June, the 5th obtains backcross progeny BC3 seed; Early September is to the BC3 that obtains and male parent 0315 sowing that backcrosses then, in early April, 2009 field examination BC3 fertility, the discovery stamen is all degenerated, gynoecium is normal, nectary is large, and nectar is many, and sterile rate and sterile degree are still 100%, and backcross with the male parent 0315 that backcrosses, in early June, the 6th obtains backcross progeny BC4 seed; In early September, the 6th is to the BC4 that obtains and male parent 0315 sowing that backcrosses, in early April, the 7th field examination BC4 fertility, stamen is all degenerated, gynoecium is normal, nectary is large, and nectar is many, and sterile rate and sterile degree are still 100%, and backcross with the male parent 0315 that backcrosses, in early June, the 7th selects 1 male sterile line CM09 that the comprehensive agronomy proterties is good and sterility is stable; The male parent that backcrosses simultaneously (0315) is the maintainer 0315 of corresponding male sterile line CM09.In early April, the 8th, 45 of hybrid combinations are joined in male sterile line CM09 and the examination of cabbage mustard inbred line, participate in then the field varietal yield test August, carry out field test, screening, combining ability test, finally select the good precocity of proterties, No. 1, auspicious a kind of sedges of the blue a kind of sedge new varieties in disease-resistant west (CM09 * 0532), precocious 3 days of this kind greener elegant blue (CK), length picking time 5 days, output 1765.3Kg/667 ㎡, greener elegant blue (CK) 1602.8Kg/667 ㎡ volume increase 10.1% viral diseases, black rot is strong, mouthfeel is crisp sweet, the comprehensive agronomy the Characters is good, sees Table 1
Table 2.
No. 1 variety biology proterties of the auspicious a kind of sedge of table 1
No. 1 variety yield of the auspicious a kind of sedge of table 2 and disease resistance
No. 1 feature of two auspicious a kind of sedges
Precocity, after field planting, 40 days can begin to gather.Plant is taller and bigger, the about 48cm of plant height, and degree of development is 50cm approximately, and minute principal stresses is strong, a kind of sedge head and the tail are well-balanced, the thick approximately 1.5cm of a kind of sedge, and single a kind of sedge is 30g heavily approximately, and a kind of sedge look light green, and meat is crisp sweet, and the water rear color and luster of scalding is emerald green, and product are of fine quality, and disease resistance is strong, reaches picking time about 28 days.
Three breedings, seeding technique main points
1 parent propagation: adopt simple plastic greenhouse to collect seed, male sterile line is cultivated with 1:2 with corresponding maintainer.Put honeybee pollination flowering stage in canopy, each booth 2 case, the florescence is pulled out maintainer later.Collect seed from the male sterile line plant and obtain maternal seed.Male parent seed collecting canopy by honeybee pollination, improves the seed collecting quality in flowering stage.
2 first generation of hybrid seeding techniques:
(1) strict isolation: must take strict quarantine measures in production of hybrid seeds base, preferably natural cover isolation, isolation distance should be more than 1500 meters.
(2) date of seeding adjustment: take male sterile line CM09 of the present invention as maternal, good inbred line 0532, carry out hybrid seeding for male parent.Because the florescence of Parent is inconsistent, in order to make the Parent flower synchronization, improve seed production when the production of hybrid seeds, need at seeding time to adjust according to the production of hybrid seeds Climate condition sowing time of Parent.
(3) field planting ratio: generally, male parent is carried out field planting with maternal according to 1:2.
(4) field management: at Jiangsu Province, general sowing mid-September, 72 holes dishes are grown seedlings, the direct field planting simple plastic greenhouse of 5-6 leaf rain shelter cultivation, when temperature reaches more than 30 ℃, surrounding is ventilated, the timely discovery of the front attention hybrid strain of blooming and pulling out.Put honeybee pollination flowering stage in canopy, each booth 2 case, the florescence pulls out male parent later and is.Strengthen the management of field damage by disease and insect.
Except above-mentioned enforcement, the present invention can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of requirement of the present invention.
Claims (8)
1. the breeding method an of precocity, the blue a kind of sedge in disease-resistant west comprises the following steps:
Step 1, take the broccoli cytoplasm male sterility line as maternal, be male parent with the good and stable selfing of broccoli, obtain first-filial generation seed F1;
Step 2, with described first-filial generation seed F1 sowing, through field test, select the male sterile line individual plant;
Step 3, the inbred line that described broccoli is good and stable are carried out 4-5 for backcross transformation as backcross male parent and described male sterile line individual plant, obtain cytoplasmic male sterile line, and the good and stable selfing of described broccoli is its maintainer;
Step 4, take described cytoplasmic male sterile line as maternal, be male parent with the good and stable selfing of cabbage mustard, hybridization obtains western blue a kind of sedge new varieties.
2. the breeding method of precocious, blue a kind of sedge in disease-resistant west according to claim 1, it is characterized in that: described broccoli cytoplasm male sterility line code name CMS055, good and the stable inbred line code name 0315 of described broccoli, described cytoplasmic male sterile line code name CM09, the inbred line code name 0532 that described cabbage mustard is good and stable.
3. the breeding method of precocious, blue a kind of sedge in disease-resistant west according to claim 1, is characterized in that: in described step 1, during hybridization, male parent and female parent are manually shelled flower bud or florescence bagging hybridization, after exposing column cap, the pollen of male parent is coated on maternal column cap, column cap is normally expanded.
4. the breeding method of precocious, blue a kind of sedge in disease-resistant west according to claim 1, it is characterized in that: in described step 3, with the cultivation of 1:2 ratio, rain shelter cultivation is adopted in cultivation to male sterile line with corresponding maintainer, keep ground moistening, temperature ventilates when surpassing 30 ℃; Put honeybee pollination flowering stage in canopy, each booth 2 case, the florescence is pulled out maintainer later.
5. the breeding method of precocious, blue a kind of sedge in disease-resistant west according to claim 1, it is characterized in that: in described step 4, seed farm is taked quarantine measures, 〉=1500 meters of distances, mid-September is selected in sowing.
6. the breeding method of precocious, blue a kind of sedge in disease-resistant west according to claim 5, it is characterized in that: when described male parent is grown to 5-6 sheet true leaf with maternal seedling stage, in the field planting of 1:2 ratio, rain shelter cultivation keeps ground moistening, ventilates when temperature surpasses 30 ℃.
7. the breeding method of precocious, blue a kind of sedge in disease-resistant west according to claim 5 is characterized in that: during the production of hybrid seeds, and the more maternal late sowing kind of male parent 5-6 days.
8. the breeding method of precocious, blue a kind of sedge in disease-resistant west according to claim 6, it is characterized in that: the florescence puts honeybee pollination in canopy, pulls out male parent after the florescence.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310131551.7A CN103168681B (en) | 2013-04-16 | 2013-04-16 | Breeding method of early-ripe and disease-resistant broccolini |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310131551.7A CN103168681B (en) | 2013-04-16 | 2013-04-16 | Breeding method of early-ripe and disease-resistant broccolini |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103168681A true CN103168681A (en) | 2013-06-26 |
| CN103168681B CN103168681B (en) | 2014-08-20 |
Family
ID=48629232
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310131551.7A Active CN103168681B (en) | 2013-04-16 | 2013-04-16 | Breeding method of early-ripe and disease-resistant broccolini |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103168681B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222422A (en) * | 2013-05-02 | 2013-07-31 | 镇江瑞繁农艺有限公司 | Breeding and seed production method for high-efficiency seed selection of late-maturing cold-resistant broccoli |
| CN103975849A (en) * | 2014-05-28 | 2014-08-13 | 镇江瑞繁农艺有限公司 | Breeding and cultivating method for early-matured, disease-resistant and loose purple cauliflowers |
| CN103988775A (en) * | 2014-05-28 | 2014-08-20 | 镇江瑞繁农艺有限公司 | Method for breeding and cultivating early ripening disease-resistant stem broccoli |
| CN105145343A (en) * | 2015-09-21 | 2015-12-16 | 镇江瑞繁农艺有限公司 | Premature, disease-resistant and stress-resistant mini broccoli breeding method |
| US11019781B2 (en) | 2016-11-04 | 2021-06-01 | Sakata Seed America, Inc. | Purple baby broccoli |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5254802A (en) * | 1987-12-17 | 1993-10-19 | Sietske Hoekstra | Male sterile brassica plants |
| CN1565170A (en) * | 2003-07-02 | 2005-01-19 | 天津科润农业科技股份有限公司蔬菜研究所 | Hybrid cauliflower cultivation method utilizing male sterility line |
| WO2008084329A2 (en) * | 2006-09-13 | 2008-07-17 | Syngenta Participations Ag | Novel rucola plants with cytoplasmic male sterility (cms) |
| CN101803564A (en) * | 2010-05-07 | 2010-08-18 | 镇江瑞繁农艺有限公司 | Simplified breeding method for preparing hybrid cabbage by using cytoplasmic male sterile lines |
| CN101810136A (en) * | 2010-05-07 | 2010-08-25 | 福州市蔬菜科学研究所 | Cauliflower cytoplasmic male sterile line breeding method and application of male sterile line |
| CN102349439A (en) * | 2011-08-25 | 2012-02-15 | 温州科技职业学院 | Breeding and seed producing method for recessive gene male sterile line of cauliflower |
| CN102835306A (en) * | 2012-09-26 | 2012-12-26 | 江苏丘陵地区镇江农业科学研究所 | Breeding method for early-maturing hybrid broccoli in additive selection |
-
2013
- 2013-04-16 CN CN201310131551.7A patent/CN103168681B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5254802A (en) * | 1987-12-17 | 1993-10-19 | Sietske Hoekstra | Male sterile brassica plants |
| CN1565170A (en) * | 2003-07-02 | 2005-01-19 | 天津科润农业科技股份有限公司蔬菜研究所 | Hybrid cauliflower cultivation method utilizing male sterility line |
| WO2008084329A2 (en) * | 2006-09-13 | 2008-07-17 | Syngenta Participations Ag | Novel rucola plants with cytoplasmic male sterility (cms) |
| US20110030081A1 (en) * | 2006-09-13 | 2011-02-03 | Syngenta Partipations Ag | Novel Rucola plants with cyptoplasmic male sterility (CMS) |
| CN101803564A (en) * | 2010-05-07 | 2010-08-18 | 镇江瑞繁农艺有限公司 | Simplified breeding method for preparing hybrid cabbage by using cytoplasmic male sterile lines |
| CN101810136A (en) * | 2010-05-07 | 2010-08-25 | 福州市蔬菜科学研究所 | Cauliflower cytoplasmic male sterile line breeding method and application of male sterile line |
| CN102349439A (en) * | 2011-08-25 | 2012-02-15 | 温州科技职业学院 | Breeding and seed producing method for recessive gene male sterile line of cauliflower |
| CN102835306A (en) * | 2012-09-26 | 2012-12-26 | 江苏丘陵地区镇江农业科学研究所 | Breeding method for early-maturing hybrid broccoli in additive selection |
Non-Patent Citations (2)
| Title |
|---|
| 周禹等: "芥蓝与甘蓝其他变种分类关系的研究", 《园艺学报》, vol. 37, no. 7, 31 December 2010 (2010-12-31), pages 1161 - 1168 * |
| 张新梅等: "甘蓝显性雄性不育基因CDMs399-3紧密连锁的分子标记", 《中国农业科学》, vol. 42, no. 11, 31 December 2009 (2009-12-31), pages 3980 - 3986 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222422A (en) * | 2013-05-02 | 2013-07-31 | 镇江瑞繁农艺有限公司 | Breeding and seed production method for high-efficiency seed selection of late-maturing cold-resistant broccoli |
| CN103222422B (en) * | 2013-05-02 | 2016-05-11 | 镇江瑞繁农艺有限公司 | Breeding and the producing method for seed of the late-maturing cold-resistant broccoli of a kind of seed selection |
| CN103975849A (en) * | 2014-05-28 | 2014-08-13 | 镇江瑞繁农艺有限公司 | Breeding and cultivating method for early-matured, disease-resistant and loose purple cauliflowers |
| CN103988775A (en) * | 2014-05-28 | 2014-08-20 | 镇江瑞繁农艺有限公司 | Method for breeding and cultivating early ripening disease-resistant stem broccoli |
| CN103988775B (en) * | 2014-05-28 | 2016-05-11 | 镇江瑞繁农艺有限公司 | A kind of precocity, disease-resistant stem breeding and the breeding method of broccoli |
| CN105145343A (en) * | 2015-09-21 | 2015-12-16 | 镇江瑞繁农艺有限公司 | Premature, disease-resistant and stress-resistant mini broccoli breeding method |
| US11019781B2 (en) | 2016-11-04 | 2021-06-01 | Sakata Seed America, Inc. | Purple baby broccoli |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103168681B (en) | 2014-08-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103975849B (en) | A kind of precocity, the breeding of disease-resistant, the pale reddish brown Broccoli of loose type and breeding method | |
| CN101480164B (en) | Method for breeding novel germplasm of Chinese cabbage with purple core | |
| CN101803564B (en) | Simplified breeding method for preparing hybrid cabbage by using cytoplasmic male sterile lines | |
| CN103168681B (en) | Breeding method of early-ripe and disease-resistant broccolini | |
| CN107912295A (en) | A kind of high-quality, high yield pumpkin new varieties breeding method | |
| CN103975848B (en) | Breeding and cultivating method for early-matured and disease-resistant brassica oleracea | |
| CN103348910B (en) | Method for transforming sterile line of Chinese kale by utilizing vernalization and generation adding | |
| CN104303987A (en) | High-yield hybrid seed production method for sweet pepper | |
| CN103461101B (en) | Stemless cucurbita maxima breeding method | |
| CN111837934A (en) | Breeding method of broccoli ball and seed variety with high glucoraphanin content | |
| CN102835306B (en) | Breeding method for early-maturing hybrid broccoli in additive selection | |
| CN103988775B (en) | A kind of precocity, disease-resistant stem breeding and the breeding method of broccoli | |
| CN104303731A (en) | Zucchini breed hybrid seed production method | |
| CN100446661C (en) | Method for seed selection of orange colored cauliflower | |
| CN107278879A (en) | A kind of breeding method of eutrophy loose type cauliflower | |
| CN103190336A (en) | Method for transforming wild cabbage into male sterility line of kohlrabi and breeding hybrids | |
| CN104170725B (en) | The selection of a kind of high yield, disease-resistant collard | |
| CN105075854A (en) | Breeding method for early-maturing and disease-resistant broccoli | |
| CN105961186A (en) | Breeding method of excellent super sweet corn inbreeding line | |
| CN104957028A (en) | High-yield hybrid seed production method of cotton | |
| CN104885910A (en) | High-yield hybrid maize seed production method | |
| CN107683764B (en) | Breeding method of purple leaf color beam waist cabbage three-line hybrid variety | |
| CN104996293A (en) | Seed production method for sweet corn hybrid--Zhetian No. 11 | |
| CN101433179A (en) | Method for producing diploid seedless water melons | |
| CN104871961A (en) | High-output hybrid seed production method for capsicum annuum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20151102 Address after: 101 No. 212000 Zhenjiang city of Jiangsu Province, Runzhou District Nan Xu Road Patentee after: JIANGSU HUIZHI INTELLECTUAL PROPERTY SERVICES CO., LTD. Address before: 212400 Zhenjiang City, Jiangsu Province Hangzhou Ning Jurong City No. 112 Patentee before: Ruifan Agricultural Gardening Co., Ltd., Zhenjiang City |
|
| C56 | Change in the name or address of the patentee | ||
| CP02 | Change in the address of a patent holder |
Address after: 212114 Zhenjiang, Zhejiang Province, Dantu high capital street, Xiangshan Road, No. 1 Patentee after: JIANGSU HUIZHI INTELLECTUAL PROPERTY SERVICES CO., LTD. Address before: 101 No. 212000 Zhenjiang city of Jiangsu Province, Runzhou District Nan Xu Road Patentee before: JIANGSU HUIZHI INTELLECTUAL PROPERTY SERVICES CO., LTD. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190424 Address after: 233400 Chuangchuang Space, 1st Floor, Chuangchuang Space, 8th Floor, 2595 Donghai Avenue, Bengbu City, Anhui Province, A-43 Chuangchuang Space Patentee after: Bengbu Yongyuan Electronic Technology Co., Ltd. Address before: No. 1 Xiangshan Avenue, Gaozai Street, Dantu District, Zhenjiang City Patentee before: JIANGSU HUIZHI INTELLECTUAL PROPERTY SERVICES CO., LTD. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20191127 Address after: 233100 Linhuai Town, Fengyang County, Chuzhou City, Anhui Province Patentee after: Anhui Yushan Grease Co., Ltd., Fengyang County Address before: 233400 Chuangchuang Space, 1st Floor, Chuangchuang Space, 8th Floor, 2595 Donghai Avenue, Bengbu City, Anhui Province, A-43 Chuangchuang Space Patentee before: Bengbu Yongyuan Electronic Technology Co., Ltd. |