CN102124941A - Method for improving seed production efficiency of hybrid rice through large-scale improvement of restorer seeds - Google Patents
Method for improving seed production efficiency of hybrid rice through large-scale improvement of restorer seeds Download PDFInfo
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Abstract
The invention belongs to the field of plant life sciences, and discloses a method for improving the seed production efficiency of hybrid rice through large-scale improvement of restorer seeds. The method comprises the following steps of: (1) cultivating large-grain restorer hybrid rice with characteristics of rice (oryza sativa) cgmcc no.3461; (2) performing grouped seed production of the large-grain restorer hybrid rice and small-grain normal grain type sterile line hybrid rice; (3) performing mixed harvesting; and (4) cleaning and separating seeds. According to the method, a large amount of seed production rice fields can be saved, the labor input in the seed production process can be reduced, the operating procedure can be simplified, and the opportunity of machine congestion can be reduced, so that the seed production cost is reduced, the seed purchase cost of rice farmers is reduced, and the income of the rice farmers is increased.
Description
Technical field
The invention belongs to the plant life scientific domain, be specifically related to the rice breeding technology field, relating to by recovering in particular is the method that seed maximization improvement improves seed production efficiency of hybrid rice.
Background technology
Hybrid rice from 1974 since China successfully realizes heterosis utilization, utilize always interval sowing father (recover system) female (male sterile line) this and gather in the crops respectively father and mother this method and use till today.Interval sowing and results father and mother are originally, not only operating technology loaded down with trivial details, increased the cost that the labour drops into, and in production of hybrid seeds process for father's height, female short good cross pollination attitude, male parent is easier utilizing " gibberellin (GA3) " to handle that the back plant is higher than female parent to cause lodging, make be difficult for when gathering in male parent in advance will lodging plant or the tillering tassel of minority lodging remove clean, thereby cause purity of hybrid to reduce (sneak into part and recover system).
The utilization of crop hybrid superiority must be can the cheap F1 of acquisition crossbreed being prerequisite, and the plantation hybrid rice can obtain output and income after the user bought crossbreed.The so-called parent that crossbreed is 2 inheritance stabilities, genotype is different, the seed that sexual hybridization produced (crossbreed F1, the complete heterozygosis of its genotype and expression power is in full accord).
The production of the present crossbreed F1 of China all is according to male sterile line (female parent) and recovers the phase of the going through fate that the sowing between system's (male parent) is extremely eared, carry out interval sowing, by stages or transplant the same period, treat to cut off male parent immediately after flowering finishes, or cutting off male parent (sneak into male parent even after flowering, just male parent is cut off) after the flowering about 25 days for preventing crossbreed in advance, gather in the crops again when the crossbreed for the treatment of on the female parent at last to be tied is ripe.The hybrid rice seed production of this mode was continued to use since 1974 35 years, only interval sowing, by stages or respectively transplant father and mother this, by stages or respectively gather in father and mother this, just will drop into 3～4/mu of labours than general Rice Production at seed farm more.
For improving seed production efficiency of hybrid rice, the scientific research personnel has done a lot of researchs, disclose a kind of method of utilizing double-particle mutant to improve propagating kind, seed production efficiency such as CN200710070955, it is by realizing improving seed production efficiency with double-particle mutant improvement male sterile line; CN99101907 discloses a kind of hybrid rice seed breeding process, and it is to improve seed production purity by the method that chemical emasculation and weeding gene import.But these methods and the application are fundamental differences.
The big grain resource " Jia Huasi " of paddy rice is passed through complex hybridization, through selective polymerization control gene obtained the big grain strain of large-scale seed, he is controlled by multiple gene, and strain self is not to producing the plant height of paddy rice, tillering ability, photosynthetic capacity, small ear fertility, breeding time etc. any bad related influence.Because big grain characteristic is subjected to controlled by multiple genes; being modified to big grain with " Jia Huasi " big grain material, to recover the F1 crossbreed of producing after the system and male sterile line be identical (is that the genotype of male sterile line is controlled because crossbreed is subjected to parent), and may be because of the recovery of a big type and contribute to some extent output with the paddy (F2 seed) that the F1 crossbreed is produced.At present both at home and abroad, still do not have that to utilize a big grain material to be used to recover be any report of improvement, remove two patent application (application number CN 200910096400.6 of inventor, " improving the seed production efficiency of hybrid rice method " and the application number CN 200910300956.2 of application on 02 26th, 2009 by kernel miniaturization improvement of male sterile line, " a kind of method of simplifying hybrid rice production of hybrid seeds program raising production of hybrid seeds production efficiency " of application on 03 19th, 2009), do not utilize the difference of seed size to mix harvesting yet, and the size of utilizing the seed of seed own is carried out the report and the invention of the letter sorting of crossbreed and male parent with the form of mesh screen.
Summary of the invention
The objective of the invention is by sexual hybridization and reasonably selection thereof, the big grain characteristic of thousand kernel weight more than 35g transduceed in any type of recovery of the paddy rice system, the seed that makes common male sterile line or granule male sterile line self obviously draws back little and big distance with the seed that recovers system, and does not change the seed size of the paddy that hybrid rice produces.So, can carry out mixed seeding male parent (recovering system), maternal (male sterile line) to simplify hybrid rice production of hybrid seeds program, at least can after finishing cross pollination, paddy rice not carry out cutting off separately of male parent, the labour who reduces in the production of hybrid seeds process drops into 1-2, thereby the reduction breeding cost reduces the rice farming and purchases the income that the cost of planting increases the rice farming.What is more important just might be carried out the mechanization production of the hybrid rice production of hybrid seeds after having realized mixing harvesting.
The present invention is achieved through the following technical solutions:
Summary of the invention
A kind of method of simplifying hybrid rice production of hybrid seeds program raising production of hybrid seeds production efficiency, it is operated according to the following steps:
(1) to recover be breeding of hybrid rice to the big grain with the good China silk CGMCC of paddy rice (Oryza sativa) No.3461 characteristic;
(2) big grain recovery is the combo production of hybrid seeds of hybrid rice and granule or normal grain type male sterile line hybrid rice;
(3) mix harvesting, when treating that promptly the crossbreed F1 that tie of institute is ripe on granule or the normal grain type male sterile line hybrid rice, mixing harvesting crossbreed F1 is seed with a big recovery;
(4) seed cleaning with separate, being that seed size is different according to crossbreed F1 with recovery promptly separates.
As preferably, according to method of the present invention, wherein, it is that breeding of hybrid rice adopted following method that described big grain recovers:
With the hybrid rice that contains big grain gene is donor parents; with common recovery be receptor parent hybridization; obtain hybrid F1 '; plantation hybrid F1 '; obtain F2 ' from generation to generation; carry big grain gene and show as big individuality at F2 ' generation cutting out partial; the individuality that shows as big grain is planted into F3 ' from generation to generation; most plant phenotypes are big grain; can stablize through the selection of 5～7 generations again and obtain the big recovery system of seed; the wherein said hybrid rice that contains big grain gene was paddy rice (Oryzasativa) Jia Huasi, was preserved in CGMCC (China Committee for Culture Collection of Microorganisms common micro-organisms center), deposit number No.3461 on November 18th, 2009.
Among the present invention, the selection of receptor parent can be a general recovery system, the seed of the paddy of tying on the first-filial generation plant that plant Jia Huasi and general recovery system hybridization back, or similar to receptor parent or greater than receptor parent.In F2 ' generation, can be isolated a certain proportion of seed shape individuality similar to donor parents, if the genetic constitution of wishing receptor parent is greater than donor parents, can in the segregation population in F1 ' generation, F2 ' generation, backcross 1～2 time by large-scale individual plant and the receptor parent of selection seed.The test cross process of remaining offspring's stability selection and recovery capability is identical with general recovery system improvement.
As preferred version, according to method of the present invention, wherein, described common recovery system is selected from bright extensive 63, Milyang 46, another name for Sichuan Province extensive 527 or continuous extensive 725.
As preferred version, according to method of the present invention, wherein, it is that its thousand kernel weight is 35g-40g that described big grain recovers, or is that the thousand kernel weight of hybrid rice manys 45-60% than common recovery.
As preferred version, according to method of the present invention, wherein, it is that male sterile line, two is male sterile line or other types male sterile line hybrid rice that described granule or normal grain type male sterile line hybrid rice are selected from three.
As more preferably scheme; according to method of the present invention; wherein; described three is that male sterile line, two is that male sterile line or other types male sterile line hybrid rice comprise 9A in the male sterile line, II-32A, extensively account for 63S, interior fragrant 2A, day rich A and conventional variety/be P13, NPB, carries the C921 granule male sterile line of granule gene sg.The described C921 that carries granule gene sg (thousand kernel weight 20g is by utilizing paddy rice (Oryza sativa) ZH-sg (in spend 11 little particle mutants) CGMCC No.2741, the granule male sterile line that obtains by this area current techique).
As preferred version; according to method of the present invention; wherein; it is when breeding time of hybrid rice differing less than 10 days that granule in the described step (2) or normal grain type male sterile line and big grain recover, the two adopt sowing successively or the same period mixed seeding, transplant and recover to be the live after a while male sterile line hybrid rice of hybrid rice or to mix and transplant in advance.
As more preferably scheme, according to method of the present invention, wherein, it is that the two adopts mixed seeding same period, mixes and transplant when breeding time of hybrid rice differing less than 10 days that granule in the described step (2) or normal grain type male sterile line and big grain recover.
As preferred version, according to method of the present invention, wherein, described is that seed size adopts the mesh screen that is fit to get final product different the separation according to crossbreed F1 with recovering.Be the crossbreed F1 of male sterile line and to recover be after the seed of selfing mixes harvesting, to be that seed size is different according to crossbreed F1 with recovery, male sterile line is to adopt suitable mesh screen to get final product separating of seed with recovering.
Detailed Description Of The Invention
The good China of paddy rice provided by the invention (sativa Oryza) silk CGMCC No.3461 is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (being called for short CGMCC) on November 18th, 2009.The big grain resource " Jia Huasi " of paddy rice is passed through complex hybridization, through selective polymerization control gene obtained the big grain strain of large-scale seed, it is controlled by multiple gene, and strain self is not to producing plant height, tillering ability, photosynthetic function, small ear fertility, breeding time etc. any bad related influence.Because big grain characteristic is subjected to controlled by multiple genes, be modified to " Jia Huasi " material and recover the F1 crossbreed of producing after the system identical with male sterile line (is that the genotype of male sterile line is controlled because crossbreed is subjected to parent), and may be because of the recovery of big grain type and contribute to some extent output with the paddy (F2 seed) that the F1 crossbreed is produced.If male parent is that thousand kernel weight is in 35 grams even the heavy recovery system of bigger grain; then can mixed seeding even live in production (production of hybrid seeds) process of crossbreed; at least can mix harvesting (father; maternal); especially mechanization mixes harvesting; harvested seed mixture can sort by suitable mesh screen when commercial seed is cleaned; distinguish crossbreed and male parent; its principle is that crossbreed is when crossing mesh screen and cleaning; because of the seed (crossbreed of knot female parent on) of the male sterile line (thousand kernel weight less than 20 grams) of common male sterile line (thousand kernel weight 25 gram) or granule by mesh screen, and thousand kernel weight to reach the above big grain recovery of 35 grams be to pass through mesh screen.
Big grain resource provided by the invention " Jia Huasi " can be by sexual hybridization and is reasonably selected, and big characteristic is imported in any different material.If big grain characteristic is imported recovery system by hybridizing and backcrossing, thereby the operating process of the hybrid rice production of hybrid seeds can be simplified the seed production efficiency of raising hybrid rice.Jia Huasi of the present invention is mainly used in the improvement of rice restorer (R), is mainly used in the sport technique segment of cross breeding seed, and can cause any harmful effect to hybrid rice production.
At present the hybrid rice of China is mainly produced crossbreed by two kinds of technological approaches, promptly utilize nuclear-matter mutually work cause " three line method " of pollen abortion or utilize " two is method " of the photoperiod-temperature sensitive genie male-sterile line that envirment factor induces.Utilize three to be or two male sterile lines that are and recovery system's hybridization (being the production of hybrid seeds), the seed of producing is exactly the F1 seed, the rice farming is exactly to buy the F1 seed to plant, and the paddy of producing (F2 seed) is processed by rice machining sector again, for the consumer provides final commodity rice.The seed that the production of hybrid seeds is produced (F1), its seed size depend on female parent (male sterile line), because the size of F1 seed is by the decision of the genotype of female parent, will bear the paddy that comes behind the F1 planting seed again, by the heterozygous genes type decision of F1.Because keystone resources of the present invention " Jia Huasi " is controlled by multiple genes, the paddy seed of bearing on the F1 plant is subjected to its male parent (recovering system) influence, and the size that its F1 sets seeds is just normal or bigger than normal slightly.
Seed cleaning is that the present crossbreed of China produces a necessary procedure later, and purpose is to remove stalk, soil block, weed seed etc.The producing and selling of relevant crop seeds, China has worked out strict rules, will be after the general cross rice production of hybrid seeds results through cleaning, promptly take out impurity such as stalk, soil block, weed seed etc. with the form (principal mode) of mesh screen.The present invention can finish separating of crossbreed F1 (granule or normal grain type) and male parent (big grain) as long as add one suitable mesh screen on separator.If with present universal hybrid rice seed separator, the different seed of seed size is separated with regard to realizing mixing results father, female parent, will greatly simplify hybrid rice production of hybrid seeds program.
The present invention has following beneficial effect:
The present invention is mainly used in the cross breeding seed sport technique segment of hybrid rice, can not have any impact to hybrid rice production, can realize:
(1) mixed seeding: utilization is subjected to the male sterile line of single recessive gene sg control or the male sterile line of common seed size to carry out the production of hybrid seeds, with the thousand kernel weight of paddy seed be combo at 35 grams even recovery bigger grain, that breeding time is suitable, male parent with maternal successively or the same period mixed seeding, or transplant male parent, live female parent after a while in advance.The labour is simplified, saved to comparable in a word present seed production way.(2) mix harvesting: need not to gather in the crops separately later male parent in this flowering of father and mother, but with grain header mixing harvesting.(3) mesh screen separates: male parent that mix the results back and crossbreed (F1) cleaning in the operation before the crossbreed packing, utilizing mesh screen to separate the thousand kernel weight difference is the different principle of seed size, the granule type tied on the male sterile line or the common grain type crossbreed recovery system with big grain can be separated.
In addition, if the big grain of rice restorer utilization material Jia Huasi carries out the production of hybrid seeds, on national aspect, can save a large amount of production of hybrid seeds rice fields; On kind of industry company aspect, can reduce the chance that labour in the production of hybrid seeds process dropped into, simplified procedures, reduces mechanical admixture, the quantity and the cost that also can reduce storage simultaneously, transport; From producing on user's aspect, it is agricultural in the cost of buying hybrid rice seed to reduce rice.
Description of drawings
Fig. 1 is that the big grain recovery of the present invention is Jia Huasi (thousand kernel weight 40g, the left side is the 1st group among the figure); And grain of rice type is hybridized in comparatively general recovery system on producing at present, bright extensive 63 (thousand kernel weight 26g a, left side is played the 2nd group among the figure), Milyang 46 (thousand kernel weight 25g a, left side is played the 3rd group among the figure); And the granule male sterile line C921 seed (thousand kernel weight 20g, rightmost among the figure) that carries granule gene sg.
Fig. 2-1 and Fig. 2 the-the 2nd, and present rice paddy seed carries out the mesh screen schematic picture of seed cleaning after breeding results, dehydration, and Fig. 2-1 is that circle hole shape mesh screen, Fig. 2-2 are the strip mesh screen.
Fig. 3 is the embodiment of the invention 1 schematic flow sheet.
Embodiment
Below in conjunction with embodiment, be described more specifically content of the present invention.Should be appreciated that enforcement of the present invention is not limited to the following examples, all will fall into protection domain of the present invention any pro forma accommodation and/or the change that the present invention made.
In the present invention, if not refer in particular to, all equipment and raw material etc. all can be buied from market or the industry is commonly used.
With Jia Huasi is donor parents (deposit number CGMCC No.3461; November 18 2009 preservation day); can be that bright extensive 63 (thousand kernel weight 26 grams), extensive 527 (thousand kernel weight 27 grams) in another name for Sichuan Province, continuous extensive 725 (thousand kernel weight 28 grams), Milyang 46 hybridization such as (thousand kernel weight 25g) recover with common recovery in China present hybrid rice production of hybrid seeds be the improvement of maximization; thousand kernel weight is increased to more than the 35g, obtain big grain and recover system.
For example, utilize bright extensive 63 with Jia Huasi hybridization, the F2 ' generation select thousand kernel weight near in addition surpass the individuality of Jia Huasi, big individuality is selected in F3 ' and continuation from generation to generation later on, the big grain that obtains at last (thousand kernel weight is 40 grams even bigger) recovers system, can (three be male sterile line with 9A in the main male sterile line in China present hybrid rice production of hybrid seeds, thousand kernel weight 26 grams), (three is male sterile line to II-32A, thousand kernel weight 28 grams), (three is male sterile line to interior fragrant 2A, thousand kernel weight 30 grams), (two is male sterile line extensively to account for 63S, or contain granule male sterile line C921 (thousand kernel weight 20g) hybridization the carrying out production of hybrid seeds of granule recessive gene sg thousand kernel weight 28 gram) etc..For example, with thousand kernel weight " the middle 9A " of 26 grams and the new restorer line of thousand kernel weight more than 40 grams by " bright extensive 63 " and Jia Huasi selection cross carries out combo, the 26 gram thousand kernel weight that its production of hybrid seeds process can be utilized male sterile line are seed size difference between 40 thousand kernel weight that restrain with recovering, and utilize mesh screen to separate to mixing harvested seed.
In the above-mentioned material, the good China of paddy rice (sativa Oryza) silk CGMCC No.3461 takes from plant, and in October, 2009, the inventor was collected in Fuyang City, Zhejiang Province China Paddy Rice Inst base, and was preserved in CGMCC on November 18th, 2009.Paddy rice is bright extensive 63, takes from plant, provides by thanking to Huaan (contact address: academy of agricultural sciences, No. 247 Fujian Province, 54 North Road, Fuzhou City, Fujian Province, postcode 350003), is formed with Gui 630 (male parent) selection cross with IR30 (female parent) by Fujian Province's Sanming City institute of agricultural sciences.9A in the paddy rice, take from plant, cultivate (excellent I A/ // excellent I B/ phenanthrene changes B//L301) and provide by Zhang Huilian (contact address: No. 359, Tiyuchang Road, Hangzhou City, Zhejiang Province, postcode 310006), be collected in Fuyang City, Zhejiang Province China Paddy Rice Inst base in September, 1997.
Embodiment 1
(1) big grain recovery is breeding of hybrid rice
Be that receptor parent, Jia Huasi are that donor parents is an example with bright extensive 63 below, embodiments of the present invention are described.
1, be that donor and common recovery are bright extensive 63 hybridization with Jia Huasi, obtain hybrid F1 ', the size of its type is between Jia Huasi and bright extensive 63, plantation hybrid F1 ', obtain F2 ', the thousand grain weigth that bear on the F2 ' generation plant present age is acted normally, and isolates and partly carries big grain material Jia Huasi gene in the F2 ' generation seed and show big individuality.Continue to select to form F3 ' strain system (or selecting the individual plant and bright extensive 63 of big grain to backcross once).In F3 ' strain system, select large-scale individual plant and the male sterile line test cross of seed, at F3 ' individual plant test cross is on the basis that male sterile line is recovered, from being, corresponding F4 ' strain selects the large-scale individual plant of seed test cross once more once more, be on the basis that male sterile line is recovered at F4 ' individual plant test cross then, from corresponding F5 ' strain is, select the large-scale individual plant of seed test cross more once more.Repeat the work of F4 ' and F5 '.Through 5-7 selection from generation to generation can obtain seed large-scale, can recover the new restorer line that it gives birth to performance to male sterile line.
Being characterized as of new restorer line: thousand kernel weight is 40 grams, and form is shown among Fig. 1 left the 1st group.
(2) production of hybrid seeds of big grain recovery system and male sterile line hybrid rice
Recovering when granule or normal grain type male sterile line hybrid rice and big grain is that hybrid rice is when carrying out the production production of hybrid seeds of crossbreed; if male sterile line (breeding time is shorter) differed 10 days and above with the breeding time of recovering system's (breeding time is longer); as select 9A in the male sterile line; still undertaken by the mode of production (successively transplanting recovery respectively is male parent and male sterile line female parent) of the present hybrid rice production of hybrid seeds; needn't carry out male parent after male parent (recovering system), maternal (male sterile line) flowering finish and cut off separately, and can mix harvesting with grain header.
(3) hybrid rice seed cleans and separates
Seed after the results is still undertaken by the dewatering type of present hybrid rice, when treating that seed moisture content reaches regulation, selection has the mesh screen of accompanying drawing 2-2 form, select suitable mesh and it is installed on the seed cleaner according to the seed width of male sterile line, the seed mixture up to standard that dewaters (the hybrid F1 that is tied on the male sterile line is the seed that selfing is tied with recovery) is dropped into seed cleaner, to separate crossbreed F1 and male parent (recovering system), its principle is that the little male sterile line of seed (F1) can pass through mesh, and the big recovery system of seed can't be separated by mesh.
Embodiment 2
(1) big grain recovery is breeding of hybrid rice
Be that receptor parent, Jia Huasi are that donor parents is an example with bright extensive 63 below, embodiments of the present invention are described.
With embodiment 1.
(2) production of hybrid seeds of big grain recovery system and male sterile line hybrid rice
When the recovery of granule male sterile line hybrid rice and big grain or normal grain type is that hybrid rice is when carrying out the production production of hybrid seeds of crossbreed; if male sterile line (breeding time is shorter) differed at 10 days with interior with the breeding time of recovering system's (breeding time is longer); as 9A in the male sterile line; can take not vernalization of male sterile line, recover is that mixed seeding is carried out in vernalization, or directly mixed seeding or mixing transplanting.Needn't carry out male parent after male parent (recovering system), maternal (male sterile line) flowering finish and cut off separately, and can mix harvesting with grain header.
(3) hybrid rice seed cleans and separates
Seed after the results is still undertaken by the dewatering type of present hybrid rice, when treating that seed moisture content reaches regulation, selection has the mesh screen of accompanying drawing 2-2 form, select suitable mesh and it is installed on the seed cleaner according to the seed width of male sterile line, the seed mixture up to standard that dewaters (the hybrid F1 that is tied on the male sterile line is the seed that selfing is tied with recovery) is dropped into seed cleaner, to separate crossbreed F1 and male parent (recovering system), its principle is that the little male sterile line of seed (F1) can pass through mesh, and the big recovery system of seed can't be separated by mesh.
Claims (9)
1. one kind is the method that seed maximization improvement improves seed production efficiency of hybrid rice by recovering, and it is characterized in that described method is operated according to the following steps:
(1) to recover be breeding of hybrid rice to the big grain with the good China silk CGMCC of paddy rice (Oryza sativa) No.3461 characteristic;
(2) big grain recovery is the combo production of hybrid seeds of hybrid rice and granule or normal grain type male sterile line hybrid rice;
(3) mix harvesting, when treating that promptly the crossbreed F1 that tie of institute is ripe on granule or the normal grain type male sterile line hybrid rice, mixing harvesting crossbreed F1 is seed with a big recovery;
(4) seed cleaning with separate, being that seed size is different according to crossbreed F1 with recovery promptly separates.
2. the method for claim 1 is characterized in that, it is that breeding of hybrid rice adopted following method that described big grain recovers:
With the hybrid rice that contains big grain gene is donor parents; with common recovery be receptor parent hybridization; obtain hybrid F1 '; plantation hybrid F1 '; obtain F2 ' from generation to generation; carry big grain gene and show as big individuality at F2 ' generation cutting out partial; the individuality that shows as big grain is planted into F3 ' from generation to generation; most plant phenotypes are big grain; can stablize through the selection of 5～7 generations again and obtain the big recovery system of seed; the wherein said hybrid rice that contains big grain gene was paddy rice (Oryzasativa) Jia Huasi, was preserved in CGMCC, deposit number No.3461 on November 18th, 2009.
3. method as claimed in claim 2 is characterized in that, described common recovery system is selected from bright extensive 63, Milyang 46, another name for Sichuan Province extensive 527 or continuous extensive 725.
4. method as claimed in claim 1 or 2 is characterized in that, it is that its thousand kernel weight is 35g-40g that described big grain recovers, or is that the thousand kernel weight of hybrid rice manys 45-60% than common recovery.
5. the method for claim 1 is characterized in that, it is that male sterile line, two is male sterile line or other types male sterile line hybrid rice that described granule or normal grain type male sterile line hybrid rice are selected from three.
6. method as claimed in claim 5; it is characterized in that; described three is that male sterile line, two is that male sterile line or other types male sterile line hybrid rice comprise 9A in the male sterile line, II-32A, extensively account for 63S, interior fragrant 2A, day rich A and conventional variety/be P13, NPB, carries the C921 of granule gene sg.
7. the method for claim 1; it is characterized in that; it is when breeding time of hybrid rice differing less than 10 days that granule in the described step (2) or normal grain type male sterile line and big grain recover, the two adopt sowing successively or the same period mixed seeding, transplant and recover to be the live after a while male sterile line hybrid rice of hybrid rice or to mix and transplant in advance.
8. as claim 1 or 7 described methods, it is characterized in that it is that the two adopts mixed seeding same period, mixes and transplant when breeding time of hybrid rice differing less than 10 days that granule in the described step (2) or normal grain type male sterile line and big grain recover.
9. the method for claim 1 is characterized in that, described is that seed size adopts the mesh screen that is fit to get final product different the separation according to crossbreed F1 with recovering.
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| CA2285579A1 (en) * | 1997-04-28 | 1998-11-05 | Wengui Yan | Crop heterosis and herbicide |
| CN1263701A (en) * | 1999-01-27 | 2000-08-23 | 黄大年 | Breeding method for hybridized rice |
| CN1115950C (en) * | 1999-04-06 | 2003-07-30 | 四川省农业科学院作物研究所 | Method for producing hybridized rice seed by screening |
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| CN112514788A (en) * | 2020-11-06 | 2021-03-19 | 湖南农业大学 | Breeding method of large-grain rice restorer line and simple seed production method of hybrid rice |
| CN112514788B (en) * | 2020-11-06 | 2023-01-20 | 湖南农业大学 | Breeding method of large-grain rice restorer line and simple seed production method of hybrid rice |
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