CN1442038A - Mechanization seed making method of hybridized rice using three line method - Google Patents
Mechanization seed making method of hybridized rice using three line method Download PDFInfo
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- CN1442038A CN1442038A CN 02104889 CN02104889A CN1442038A CN 1442038 A CN1442038 A CN 1442038A CN 02104889 CN02104889 CN 02104889 CN 02104889 A CN02104889 A CN 02104889A CN 1442038 A CN1442038 A CN 1442038A
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Abstract
A mechanized method for preparing the seeds of three-line hybridized paddy rice includes such steps as hybridizing the rice whose husk color is different from rormal color with the maintenance line whose husk color is normal to obtain the maintenance-line seeds whose husk color is different from normal color; transfer culturing of the sterile-line seeds whose husk color is different from normal color, mixing the maintenance-line seeds with the sterile-line seeds, mechanical sowing, harvesting; and further using color selecting machine for color separation to obtain the hybridized seeds and recovery-line seeds.
Description
Technical field
The invention belongs to rice breeding, relate to breeding technology for hybrid rice, particularly the method for series of three-series hybrid rice seed is produced in mechanization.
Background technology
Paddy rice (Oryza sativa L.) heterosis utilization is divided into three line method and two kinds of methods of two line method, and heterosis utilization is mainly based on three line method at present.The main path of three line method rice heterosis utilization is by cultivation male sterile series (A), male sterility maintainer line (B), and male sterile restoring line (R) (being collectively referred to as three is) reaches the purpose that the strong advantage of apolegamy makes up.As far back as 1958, it is clear that northeastern Japan university wins tail, carried out the research of male sterile line for utilizing rice heterosis, finds that the wild rice of red awns of China can cause the rattan slope to produce male sterile No. 5.It is 65 hybridization in maternal and the platform that the long friend of Japanese Ryukyu university new city in 1966 covers II with the Ursula one of admiring, breed 65 male sterile lines in the BT type platform, can educate strain to the part in 65 male sterile lines in the platform simultaneously and recover system through the stable homogeneity of having bred BT type male sterile line of selfing, nineteen sixty-eight is realized three series mating.Subsequently, thus No. 5 hybridization of special rice and rattan slope in the Japanese agriculture technical research Burma rice variety, through continuous backcross bred have in the combination of special type male sterile line preparation, but all do not fail by force to be applied to produce because of advantage.The research of Chinese hybrid rice starts from 1964, under Yuan Longping's leader, realized the indica hybrid rice three series mating in 1973, realized the japonica hybrid rice three series mating in 1975 in the north, from then on series of three-series hybrid rice in state-owned development rapidly, in the combination seed selection, series of three-series hybrid rice all is enhanced on output, rice matter and resistance through the development of decades.
Yet the development of hybrid rice be unable to do without breeding of hybrid rice.
Breeding of hybrid rice need be set up the breeding field of male sterile line and the seed farm of crossbreed.
In sterile breeding field, with sterile be maternal, to keep be male parent, sowing respectively than alternate plantation respectively, is regulated flowering stage by certain row; Powder is manually caught up with in employing in flowering stage, makes male sterile line accept the pollen of maintenance line, and it is solid to be fertilized, and maintenance line is self-fertility then, then harvesting respectively.On the male sterile line plant, receive seed still for male sterile line, stay wherein a part for continue breeding male sterile lines with outside, all the other major parts are used for seed farm preparing hybrid kind; Having planted that the maintenance line selfing is tied still is maintenance line.
In seed farm with sterile be maternal, to recover be male parent, sowing respectively, than alternate plantation respectively, regulate flowering stage by certain row, adopt in flowering stage and manually catch up with powder, make male sterile line accept to recover the pollen of system, it is solid to be fertilized, and recovery is a self-fertility, then harvesting respectively, producing hybrid seed (seed of bearing on the male sterile line) and recovery is seed (recovery is a seed that selfing is tied, still for recovering system).
Since Chinese series of three-series hybrid rice was supporting, various places were groped, are created and accumulated extremely rich experience in the practice of the production of hybrid seeds, and hybrid seed yield is had increased significantly.But breeding technology for hybrid rice and the development preliminary phase variation more basic for a long time than not having, still adopt the sowing of above-described this difference of father and mother phase, powder, technical measures such as harvesting are respectively planted, manually caught up with in the branch railway carriage or compartment, and seed production efficiency is not high, labour intensity is big, and seed quality also is difficult to control simultaneously.How to improve this seed production technique system, become hybrid rice further develop and improve China's seed international competitiveness and hybrid rice technology developed countries such as the U.S. further promote one of the important topic that must solve.Enlarging production of hybrid seeds scale is to reduce production costs, and improves the key of production of hybrid seeds benefit and assurance seed quality, realizes that the mechanization of hybrid rice seeds production then is the only way of the scale production of hybrid seeds.
Summary of the invention
Technical problem to be solved by this invention is the mechanization that hybrid rice seeds is produced, overcome above-mentioned plant respectively, manually catch up with powder, harvesting respectively, seed production efficiency is low, labour intensity is big, and the not high drawback of seed production quality provides mechanization seed making method of hybridized rice using three line method.
The technical solution used in the present invention, its special character is:
1. select the rice varieties of husk colouring discrimination for use in normal husk look, with the normal paddy rice maintenance line inter breed crossing of husk color, obtain the husk colouring discrimination in the paddy rice maintenance line seed of normal husk look, and then transformation goes out the rice sterile line seed of husk colouring discrimination in normal husk look;
2. use of the rice sterile line seed and the mixed seeding of the normal rice restorer seed machinery of husk color of described husk colouring discrimination in normal husk look, catch up with powder in flowering stage, it is seed in the crossbreed seed of normal husk look and normal recovery of husk color of selfing that receipts husk colouring discrimination is mixed by machinery in solid back, use the color selector sorting again, obtaining described crossbreed seed and recovery respectively is seed.
Producing method for seed of the present invention, consider to comprise the propagation method of husk colouring discrimination from optimizing: promptly in the paddy rice maintenance line of normal husk look, adopt transgenic technology to import single recessive weed killer herbicide lethal gene at described husk colouring discrimination in the male sterile line of normal husk look, import the male-sterile seed machinery mixed seeding of this maintenance line seed of this gene and described husk colouring discrimination in normal husk look, catch up with powder in flowering stage, kill maintenance line by spray herbicide then, the solid back of male sterile line mechanical harvesting obtains the husk colouring discrimination in the male-sterile seed of normal husk look.
Producing method for seed of the present invention is considered also to comprise the propagation method of husk colouring discrimination in the maintenance line of normal husk look from optimizing: promptly get described husk colouring discrimination and adopt mechanical planting, harvesting to realize in the maintenance line seed of normal valley look.
Producing method for seed of the present invention, from more optimize considering, can comprise described husk colouring discrimination in the propagation method of the male sterile line of normal husk look and husk colouring discrimination in the propagation method of the maintenance line of normal husk look.
Of the present inventionly catch up with powder to be meant that the air-flow that produces with machinery or wind-force catches up with powder in flowering stage.
The present invention is when hybridizing with the maintenance line of husk color normal (being called for short " normal look "), what select for use is that the husk colouring discrimination is in normal husk look one rice varieties of (being called for short " heterochromatic "), this rice varieties is a kind of recessive monogenic kind of being with, with it as husk color mark kind.Through hybridization and backcross that (described transformation is meant and backcrosses, be filial generation and parent hybridization), can obtain the husk colouring discrimination in maintenance line (heterochromatic maintenance line) seed of normal husk look and husk colouring discrimination in male sterile line (heterochromatic male sterile line) seed of normal husk look.
" normal look " plants with the hybridization of " heterochromatic " kind can obtain " heterochromatic " offspring.
Male sterile line, maintenance line, recovery system are used for producing hybrid rice; Utilize hybrid vigour, can produce high yield, high-quality paddy rice.
When utilizing hybrid vigour to carry out paddy rice cross breeding production crossbreed, with male sterile line and recovery line hybrid breeding.The present invention adopts heterochromatic male sterile line and normal look to recover system's hybridization, use mechanical mixed seeding, machinery or catch up with the solid back machinery of powder to mix by wind-force and receive when blooming, in the gained seed, its husk colouring discrimination of hybrid seed is in normal husk look, and it is that its husk color of seed that selfing is tied is normal husk look that normal look recovers, thus, can sub-elect the crossbreed seed and recovery is a seed with color selector, realize broadcasting slotting, catch up with powder, harvesting, seed separation mechanization.
Described normal husk look is meant at present the generally husk color that paddy presented of production, and described husk colouring discrimination is meant that in normal husk look husk color and normal husk look have big aberration or remarkable aberration.
The present invention at first makes heterochromatic maintenance line and heterochromatic male sterile line, adopts heterochromatic male sterile line when producing hybrid seed.
Be each hybrid seeding of energy, heterochromatic male sterile line needs breeding.Heterochromatic male sterile line and the hybridization of heterochromatic maintenance line are adopted in the breeding of heterochromatic male sterile line.For realizing mechanical mixed seeding, mixing and receive, adopt transgenic technology that single recessive weed killer herbicide lethal gene is imported in the heterochromatic maintenance line, with the mixed seeding of heterochromatic male sterile line machinery, machinery was caught up with powder or was utilized wind-force to catch up with powder flowering stage, herbicide spraying kills maintenance line again, use the mechanical harvesting male sterile line then, obtain male-sterile seed.
Mechanical planting is adopted in the breeding of heterochromatic maintenance line, gathers in the crops behind the self-fertility.
The present invention is owing to adopt the heterochromatic male sterile line of paddy rice, heterochromatic maintenance line, when recovering system's hybridization with heterochromatic male sterile line and Chang Se, catch up with powder with mechanical mixed seeding, machinery, solid back machinery mixes to be received, mix the rice paddy seed of receiving with the color selector sorting again, obtaining hybrid rice seed (can be used for the land for growing field crops produces) and recovery is seed, plant, manually catch up with operations such as powder, people's work point receipts so when the paddy rice cross breeding production of hybrid seeds, abolished people's work point, improved the paddy rice cross breeding seed production efficiency greatly, save man-hour, save the labour, and improved seed production quality.Adopt transgenic technology the present invention with weed killer herbicide gene importing maintenance line, then available mechanical system breeding male sterile lines, thus the entire mechanization that can realize the hybrid rice three line for hybrid seed production is operated.
Embodiment
Mechanization seed making method of hybridized rice using three line method, adopting the husk color is that brown (has big aberration with normal husk look, as brown husk mark) the blue or green B early of indica type wide affine kind Dular and the normal maintenance line of husk color association hybridization, the maintenance line of a husk color of seed selection brown therefrom, and and then transformation become the male sterile line (being referred to as " look selects A ") of husk color brown; When selecting A and husk color to recover line hybrid breeding normally with look, it is live to adopt aircraft to mix, catch up with powder in flowering stage with the air-flow that helicopter produces, solid back mixes receipts crossbreed seed with machinery and recovery is a seed, and crossbreed seed and normal recovery of husk color of utilizing aberration to sub-elect husk color brown with color selector is seed again; In the maintenance line of husk color brown, import single recessive Bentazon lethal gene, when breeding male sterile lines, adopt aircraft to mix the male-sterile seed of live husk color brown and the maintenance line seed of husk color brown, catch up with powder in flowering stage with the air-flow that helicopter produces, kill maintenance line by spraying Bentazon then, mechanical harvesting is used in the solid back of male sterile line, obtains the male-sterile seed of husk color brown; Mechanical planting, transplanting, results are also adopted in breeding to the maintenance line of husk color brown, realize the entire mechanization production of hybrid seeds that the series of three-series hybrid rice seed is produced.
Claims (4)
1, mechanization seed making method of hybridized rice using three line method is characterized in that:
1. select the rice varieties of husk colouring discrimination for use in normal husk look, with the normal paddy rice maintenance line inter breed crossing of husk color, obtain the husk colouring discrimination in the paddy rice maintenance line seed of normal husk look, and then transformation goes out the rice sterile line seed of husk colouring discrimination in normal husk look;
2. use of the rice sterile line seed and the mixed seeding of the normal rice restorer seed machinery of husk color of described husk colouring discrimination in normal husk look, catch up with powder in flowering stage, it is seed in the crossbreed seed of normal husk look and normal recovery of husk color of selfing that receipts husk colouring discrimination is mixed by machinery in solid back, use the color selector sorting again, obtaining described crossbreed seed and recovery respectively is seed.
2, producing method for seed as claimed in claim 1, it is characterized in that comprising the propagation method of husk colouring discrimination: in the paddy rice maintenance line of normal husk look, adopt transgenic technology to import single recessive weed killer herbicide lethal gene at described husk colouring discrimination in the male sterile line of normal husk look, import the male-sterile seed machinery mixed seeding of this maintenance line seed of this gene and described husk colouring discrimination in normal husk look, catch up with powder in flowering stage, kill maintenance line by spray herbicide then, the solid back of male sterile line mechanical harvesting obtains the husk colouring discrimination in the male-sterile seed of normal husk look.
3, producing method for seed as claimed in claim 1 or 2 is characterized in that comprising the propagation method of husk colouring discrimination in the maintenance line of normal husk look: get described husk colouring discrimination and adopt mechanical planting, harvesting to realize in the maintenance line seed of normal valley look.
4, producing method for seed as claimed in claim 1 or 2 is characterized in that describedly catching up with powder to be meant that the air-flow that produces with machinery or wind-force catches up with powder in flowering stage.
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| CN 02104889 CN1442038A (en) | 2002-03-04 | 2002-03-04 | Mechanization seed making method of hybridized rice using three line method |
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| CN 02104889 CN1442038A (en) | 2002-03-04 | 2002-03-04 | Mechanization seed making method of hybridized rice using three line method |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100544590C (en) * | 2007-08-22 | 2009-09-30 | 浙江省农业科学院 | Utilize double-particle mutant to improve the method for propagating kind, seed production efficiency |
| CN102342243A (en) * | 2010-07-28 | 2012-02-08 | 安徽省农业科学院水稻研究所 | Production method for mechanized production of rice seed |
| CN102124941B (en) * | 2010-01-14 | 2012-07-25 | 中国水稻研究所 | Method for improving seed production efficiency of hybrid rice through large-scale improvement of restorer seeds |
| CN103493726A (en) * | 2013-10-21 | 2014-01-08 | 安徽省农业科学院水稻研究所 | Method for mechanized seed production of hybrid rice through integration of seedling slinging technology and direct seeding technology |
| CN103518619A (en) * | 2013-10-21 | 2014-01-22 | 安徽省农业科学院水稻研究所 | Hybrid rice variety breeding and producing method suitable for safe, high-efficiency and mechanized seed production |
| EP2781151A1 (en) * | 2013-03-18 | 2014-09-24 | Bayer CropScience AG | Methods of separating hybrid seed from a mixture of seeds |
| CN106455510A (en) * | 2014-03-10 | 2017-02-22 | 利马格兰集团 | Hybrid cereal seed production |
| CN107889743A (en) * | 2017-11-26 | 2018-04-10 | 安徽省农业科学院水稻研究所 | A kind of seed selection of two-line sterile line of suitable mechanization Mixed plant and Mixed plant method |
| CN112740967A (en) * | 2021-01-29 | 2021-05-04 | 新疆农垦科学院 | Seed production method for three-line hybrid wheat precision hole sowing |
-
2002
- 2002-03-04 CN CN 02104889 patent/CN1442038A/en active Pending
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100544590C (en) * | 2007-08-22 | 2009-09-30 | 浙江省农业科学院 | Utilize double-particle mutant to improve the method for propagating kind, seed production efficiency |
| CN102124941B (en) * | 2010-01-14 | 2012-07-25 | 中国水稻研究所 | Method for improving seed production efficiency of hybrid rice through large-scale improvement of restorer seeds |
| CN102342243A (en) * | 2010-07-28 | 2012-02-08 | 安徽省农业科学院水稻研究所 | Production method for mechanized production of rice seed |
| US10028460B2 (en) | 2013-03-18 | 2018-07-24 | Bayer Cropscience Ag | Methods of separating hybrid seed from a mixture of seeds |
| US10743486B2 (en) | 2013-03-18 | 2020-08-18 | Basf Se | Methods of separating hybrid seed from a mixture of seeds |
| EP2781151A1 (en) * | 2013-03-18 | 2014-09-24 | Bayer CropScience AG | Methods of separating hybrid seed from a mixture of seeds |
| WO2014147005A1 (en) * | 2013-03-18 | 2014-09-25 | Bayer Cropscience Ag | Methods of separating hybrid seed from a mixture of seeds |
| CN103493726A (en) * | 2013-10-21 | 2014-01-08 | 安徽省农业科学院水稻研究所 | Method for mechanized seed production of hybrid rice through integration of seedling slinging technology and direct seeding technology |
| CN103518619B (en) * | 2013-10-21 | 2015-09-09 | 安徽省农业科学院水稻研究所 | A kind of hybrid rice variety seed selection and production method being suitable for the mechanization production of hybrid seeds |
| CN103518619A (en) * | 2013-10-21 | 2014-01-22 | 安徽省农业科学院水稻研究所 | Hybrid rice variety breeding and producing method suitable for safe, high-efficiency and mechanized seed production |
| CN106455510A (en) * | 2014-03-10 | 2017-02-22 | 利马格兰集团 | Hybrid cereal seed production |
| AU2015228940B2 (en) * | 2014-03-10 | 2021-07-22 | Limagrain Europe | Hybrid cereal seed production |
| US11737404B2 (en) | 2014-03-10 | 2023-08-29 | Limagrain Europe | Hybrid cereal seed production |
| CN107889743A (en) * | 2017-11-26 | 2018-04-10 | 安徽省农业科学院水稻研究所 | A kind of seed selection of two-line sterile line of suitable mechanization Mixed plant and Mixed plant method |
| CN112740967A (en) * | 2021-01-29 | 2021-05-04 | 新疆农垦科学院 | Seed production method for three-line hybrid wheat precision hole sowing |
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