CN107466842A - The selection of Indica round-grained rice osculant photo-thermo-sensitive genic male sterile line - Google Patents
The selection of Indica round-grained rice osculant photo-thermo-sensitive genic male sterile line Download PDFInfo
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- CN107466842A CN107466842A CN201710796640.1A CN201710796640A CN107466842A CN 107466842 A CN107466842 A CN 107466842A CN 201710796640 A CN201710796640 A CN 201710796640A CN 107466842 A CN107466842 A CN 107466842A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention belongs to rice breeding field, discloses a kind of selection of Indica round-grained rice osculant photo-thermo-sensitive genic male sterile line, is related to the utilization that rice two is subspecies indica and japonica hybrid advantage.The purpose of the present invention is to utilize photoperiod-temperature sensitive genie male-sterile line donor gene, passes through indica-japonica hybrid seed selection Xian round-grained rice osculant photo-thermo-sensitive genic male sterile lines.This two-line sterile line has a certain proportion of japonica rice genome composition, with indica restorer line combo can the ideotype breeding of the strong hybrid vigour of seed selection combine；Japonica rice genome component ratio is small, and photonasty is weak, reduces risk in hybrid seed production.The features such as not restricted with reference to two-line hybrid rice combo freedom and by Rescued virus, it is ideotype breeding combination using this sterile line breeding two, while the utilization of rice indica subspecies Heterosis is realized, the limitation of the subspecies indica and japonica hybrid advantage germplasm utilization of resources has been widened significantly.
Description
Technical field
The invention belongs to rice breeding field, more particularly to the utilization of double-linear hybrid rice subspecies indica and japonica hybrid advantage.
Background technology
The photo-thermo-sensitive genic male sterile line of the existing large-area applications in China is mainly Nongken 58S, peace agriculture S-1 and weighs agriculture S-1 etc. no
Educate the Derivative line for being, such as Peiai 64S and Y58S.Also include the sterile line such as Guangzhan 63S, strain 1S in other sources.From existing light
The affiliation of temperature-sensitive sterile line sees that most of two-line sterile line has japonica rice blood relationship, as Peiai 64S has japonica rice land reclamation and cultivation 58
Genetic constitution, and Y58S is derived by Peiai 64S.But in terms of its phenotype, the temperature sensitive core of light of existing large-area applications is not
It is to belong to indica type two-line sterile line to educate, the bottleneck so that yield level of existing two-line hybrid rice combination reach a certain height.
Ideotype breeding has the yield potential than indica type or japonica hybrid rice volume increase 15-20%.Intend further improving rice
Yield level, it is an effective way using indica-japonica heterosis, and typical indica-japonica hybrid often has breeding time long, solid
The low many unfavorable performances of rate make a miscellaneous generation be difficult to apply in production.Therefore, seed selection Xian round-grained rice genetic constitution matches suitable Xian
Round-grained rice osculant photo-thermo-sensitive genic male sterile line is the inclined Xian or inclined rice with japonica rice of Xian round-grained rice osculants to match two, is overcoming typical indica-japonica hybrid
While unfavorable factor, the hybrid vigour of indica-japonica hybrid can be played again, further improves rice yield.
The content of the invention
It is an object of the invention to provide a kind of selection of Xian round-grained rice osculant photo-thermo-sensitive genic male sterile line, further to carry
High hybrid rice yield potential, two development for being ideotype breeding are promoted to establish material foundation.
To achieve the above object, the technical solution adopted by the present invention is：A kind of Indica round-grained rice osculant photoperiod-temperature sensitive genie male-sterile line
The selection of system, step include：
(1), the initiative of Xian round-grained rice osculant material：Hybridized using the high-quality Indica Rice material of rice quality and japonica rice material
F1 is obtained, is returned once using F1 and Indica Rice material, obtains the BC1F1 of 3/4 long-grained nonglutinous rice genetic constitution of tool, plantation BC1F1 is obtained
BC1F2, plantation BC1F2 colonies, separated from it selected in offspring compact plant type, plant height 65-75cm, long fringe type, middle long grain type,
The material of the leaf color Xian round-grained rice osculant indica weak compared with deep, photonasty, through 4-5 generation selfing final election stable moldings；
(2) phto-temperature reaction, is imported：Utilize the above-mentioned Xian round-grained rice osculant indica breeding materials chosen and stablized
Material, with being hybridized using round-grained rice type photo-thermo-sensitive genic male sterile line as female parent, obtain the F1 for importing phto-temperature reaction；
(3), the seed selection of Xian round-grained rice osculant photo-thermo-sensitive genic male sterile line：The F2 population materials of above-mentioned (2) acquisition are planted, in positive season
Under long day hot conditions, seed selection plant height 65-75cm；Plant type is compact；Plant and leaf shape japonica-cline type, seedling leaf color are deeper；The inclined Xian of fringe grain type
Type, grain length-width ratio 3:1；Tillering ability is relatively strong, individual plant effective tillering 10-12；Breeding time is short 65-75 days；It is early during flower, same day collection
Middle flowering time is in the morning 10:30-11:30；Stigma appearing reaches 75%-85%；Thin thin, the I-KI dyeing microscopic examinations of flower pesticide are sterile
Sterile line character strain, 4-5 is for stable molding for selfing；Stable molding sterile line strain is carried out with long-grained nonglutinous rice or Xian round-grained rice osculants material
It is measured hybridization, the Xian round-grained rice osculant photo-thermo-sensitive genic male sterile lines of the strong advantage Hybrid Rice Combination of seed selection energy combo.
The method have the benefit that：The present invention is the important supplement and development to Chinese double-linear hybrid rice, is led to
The suitable Xian round-grained rice osculant photo-thermo-sensitive genic male sterile line of seed selection Xian round-grained rice genetic constitution ratios is crossed, it is solid to overcome typical Indica-japonica hybridization
The unfavorable factors such as rate is low, breeding time is long, it is ideotype breeding with long-grained nonglutinous rice or Xian round-grained rice intermediate materials combo two, from Xian round-grained rice
The utilization of subspecies indica and japonica hybrid advantage is realized in the proportioning of genome composition, is that the development of ideotype breeding carries for two
Technical support and material foundation are supplied.
Embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all
Belong to the scope of protection of the invention.
Embodiment 1
(1), the initiative of Xian round-grained rice osculant material：Hybridized using the high-quality Indica Rice material of rice quality and japonica rice material
F1 is obtained, is returned once using F1 and Indica Rice material, obtains the BC1F1 of 3/4 long-grained nonglutinous rice genetic constitution of tool, plantation BC1F1 is obtained
BC1F2 is obtained, plantation BC1F2 colonies, is separated from it and compact plant type, plant height 65cm, long fringe type, middle long grain type, leaf is selected in offspring
The material of the color Xian round-grained rice osculant indica weak compared with deep, photonasty, final election stable molding is selfed through 4 generations；
(2) phto-temperature reaction, is imported：Utilize the above-mentioned Xian round-grained rice osculant indica breeding materials chosen and stablized
Material, with being hybridized using round-grained rice type photo-thermo-sensitive genic male sterile line as female parent, obtain the F1 for importing phto-temperature reaction；
(3), the seed selection of Xian round-grained rice osculant photo-thermo-sensitive genic male sterile line：The F2 population materials of above-mentioned (2) acquisition are planted, in positive season
Under long day hot conditions, seed selection plant height 65cm；Plant type is compact；Plant and leaf shape japonica-cline type, seedling leaf color are deeper；Fringe grain type indica,
Grain length-width ratio 3:1；Tillering ability is relatively strong, individual plant effective tillering 10-12；Breeding time is short 65 days；Early during flower, same day concentration is bloomed
Time is in the morning 10:30-11:30；Stigma appearing is up to 75%；Thin thin, the I-KI dyeing microscopic examinations of flower pesticide are sterile sterile line character
Strain, it is selfed 4 generation stable moldings；Stable molding sterile line strain carries out being measured hybridization with long-grained nonglutinous rice or Xian round-grained rice osculant materials, selects
Educate the Xian round-grained rice osculant photo-thermo-sensitive genic male sterile lines of the energy strong advantage Hybrid Rice Combination of combo.
Embodiment 2
(1), the initiative of Xian round-grained rice osculant material：Hybridized using the high-quality Indica Rice material of rice quality and japonica rice material
F1 is obtained, is returned once using F1 and Indica Rice material, obtains the BC1F1 of 3/4 long-grained nonglutinous rice genetic constitution of tool, plantation BC1F1 is obtained
BC1F2 is obtained, plantation BC1F2 colonies, is separated from it and compact plant type, plant height 75cm, long fringe type, middle long grain type, leaf is selected in offspring
The material of the color Xian round-grained rice osculant indica weak compared with deep, photonasty, final election stable molding is selfed through 5 generations；
(2) phto-temperature reaction, is imported：Utilize the above-mentioned Xian round-grained rice osculant indica breeding materials chosen and stablized
Material, with being hybridized using round-grained rice type photo-thermo-sensitive genic male sterile line as female parent, obtain the F1 for importing phto-temperature reaction；
(3), the seed selection of Xian round-grained rice osculant photo-thermo-sensitive genic male sterile line：The F2 population materials of above-mentioned (2) acquisition are planted, in positive season
Under long day hot conditions, seed selection plant height 75cm；Plant type is compact；Plant and leaf shape japonica-cline type, seedling leaf color are deeper；Fringe grain type indica,
Grain length-width ratio 3:1；Tillering ability is relatively strong, individual plant effective tillering 10-12；Breeding time is short 75 days；Early during flower, same day concentration is bloomed
Time is in the morning 10:30-11:30；Stigma appearing is up to 85%；Thin thin, the I-KI dyeing microscopic examinations of flower pesticide are sterile sterile line character
Strain, it is selfed 5 generation stable moldings；Stable molding sterile line strain carries out being measured hybridization with long-grained nonglutinous rice or Xian round-grained rice osculant materials, selects
Educate the Xian round-grained rice osculant photo-thermo-sensitive genic male sterile lines of the energy strong advantage Hybrid Rice Combination of combo.
Embodiment 3
Xian round-grained rice osculant photo-thermo-sensitive genic male sterile lines Zhejiang section 82S seed selection：Utilize the high-quality long-grained nonglutinous rice material C P231 of rice quality
With photonasty is weak, ripe phase, the hybridization of japonica rice material morning round-grained rice 305 obtained F1 earlier, then be returned once, obtain using CP231 to be maternal
BC1F1, harvest and plant BC1F2 colonies, separated from it and select leaf morphology good in offspring, grain type is partially long, and leaf color is relatively deep and feels
The material of the weak Xian round-grained rice osculant indicas of photosensitiveness, the Xian round-grained rice osculant indicas of stable molding are obtained through 4-5 generation selfing final elections
Material GS2066.Using Japonica type PGMS line 7001S as female parent, hybridize with Xian round-grained rice osculant materials GS2066 and import Genetic Sterility
Gene simultaneously obtains F1, under positive Ji Chang hot conditions, in F2 colonies seed selection meet two-line sterile line character requirement and photosensitive
Property weak Xian round-grained rice osculant photo-thermo-sensitive genic male sterile line strains, through 4-5 generation selfing final election shapings.Stable molding sterile line strain and Xian
Rice or Xian round-grained rice osculant materials carry out being measured screening by hybridization, have selected Xian round-grained rice osculant photo-thermo-sensitive genic male sterile lines Zhejiang section 82S.
It should be noted last that：Above example is only to illustrative and not limiting technical scheme, although ginseng
The present invention is described in detail according to above-described embodiment, it will be apparent to an ordinarily skilled person in the art that：Still can be to this
Invention is modified or equivalent substitution, and any modification or partial replacement without departing from the spirit and scope of the present invention, its is equal
It should cover among scope of the presently claimed invention.
Claims (1)
1. the selection of Indica round-grained rice osculant photo-thermo-sensitive genic male sterile line, it is characterized in that, step includes：
(1), the initiative of Xian round-grained rice osculant material：Obtained using the high-quality Indica Rice material of rice quality and the hybridization of japonica rice material
F1, it is returned once using F1 and Indica Rice material, obtains the BC1F1 of 3/4 long-grained nonglutinous rice genetic constitution of tool, plantation BC1F1 is obtained
BC1F2, plantation BC1F2 colony, separate from it and compact plant type, plant height 65-75cm, long fringe type, middle long grain type, leaf are selected in offspring
The material of the color Xian round-grained rice osculant indica weak compared with deep, photonasty, through 4-5 generation selfing final election stable moldings；
(2) phto-temperature reaction, is imported：Chosen and stable Xian round-grained rice osculant indica breeding materials using above-mentioned, with
Hybridized using round-grained rice type photo-thermo-sensitive genic male sterile line as female parent, obtain the F1 for importing phto-temperature reaction；
(3), the seed selection of Xian round-grained rice osculant photo-thermo-sensitive genic male sterile line：The F2 population materials of above-mentioned (2) acquisition are planted, in positive Ji Chang
Under hot conditions, seed selection plant height 65-75cm；Plant type is compact；Plant and leaf shape japonica-cline type, seedling leaf color are deeper；Fringe grain type indica, paddy
Grain length-width ratio 3:1；Tillering ability is relatively strong, individual plant effective tillering 10-12；Breeding time is short 65-75 days；Early during flower, same day concentration is opened
Take time in the morning 10:30-11:30；Stigma appearing reaches 75%-85%；Thin thin, the I-KI dyeing microscopic examinations of flower pesticide are sterile infertility
It is character strain, 4-5 is for stable molding for selfing；Stable molding sterile line strain is measured with long-grained nonglutinous rice or Xian round-grained rice osculant materials
Hybridization, the Xian round-grained rice osculant photo-thermo-sensitive genic male sterile lines of the strong advantage Hybrid Rice Combination of seed selection energy combo.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107960319A (en) * | 2017-11-23 | 2018-04-27 | 安徽省绿福农业科技股份有限公司 | A kind of long grain round-grained rice rice variety selective and screening technique |
| CN112753564A (en) * | 2020-12-29 | 2021-05-07 | 湖南省春云农业科技股份有限公司 | Breeding method of high-quality two-line sterile line of rice |
| CN113728911A (en) * | 2021-03-12 | 2021-12-03 | 华南农业大学 | Novel rice high-temperature reversible sterile line and two-line hybrid rice seed production system |
| CN116058276A (en) * | 2022-07-19 | 2023-05-05 | 湖北大学 | Method for preparing strong-dominance indica-japonica intersubular hybrid rice by utilizing tetraploid rice restored diploid and application thereof |
-
2017
- 2017-09-06 CN CN201710796640.1A patent/CN107466842A/en active Pending
Non-Patent Citations (1)
| Title |
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| 余守武等: "籼粳中间型水稻光温敏核不育系浙科82S的选育", 《分子植物育种》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107960319A (en) * | 2017-11-23 | 2018-04-27 | 安徽省绿福农业科技股份有限公司 | A kind of long grain round-grained rice rice variety selective and screening technique |
| CN112753564A (en) * | 2020-12-29 | 2021-05-07 | 湖南省春云农业科技股份有限公司 | Breeding method of high-quality two-line sterile line of rice |
| CN113728911A (en) * | 2021-03-12 | 2021-12-03 | 华南农业大学 | Novel rice high-temperature reversible sterile line and two-line hybrid rice seed production system |
| CN113728911B (en) * | 2021-03-12 | 2022-05-31 | 华南农业大学 | Novel rice high-temperature reversible sterile line and two-line hybrid rice seed production system |
| CN116058276A (en) * | 2022-07-19 | 2023-05-05 | 湖北大学 | Method for preparing strong-dominance indica-japonica intersubular hybrid rice by utilizing tetraploid rice restored diploid and application thereof |
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