CN109757359B - Method for cultivating high-quality fragrant long-grain japonica rice - Google Patents
Method for cultivating high-quality fragrant long-grain japonica rice Download PDFInfo
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- CN109757359B CN109757359B CN201910213060.4A CN201910213060A CN109757359B CN 109757359 B CN109757359 B CN 109757359B CN 201910213060 A CN201910213060 A CN 201910213060A CN 109757359 B CN109757359 B CN 109757359B
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- 240000008467 Oryza sativa Japonica Group Species 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims description 15
- 235000013339 cereals Nutrition 0.000 claims abstract description 30
- 125000003118 aryl group Chemical group 0.000 claims abstract description 9
- 238000012364 cultivation method Methods 0.000 claims abstract description 7
- 239000000796 flavoring agent Substances 0.000 claims abstract description 3
- 241000196324 Embryophyta Species 0.000 claims description 33
- 235000007164 Oryza sativa Nutrition 0.000 claims description 24
- 235000009566 rice Nutrition 0.000 claims description 24
- 239000003205 fragrance Substances 0.000 claims description 18
- 238000009396 hybridization Methods 0.000 claims description 17
- 244000184734 Pyrus japonica Species 0.000 claims description 14
- 238000000926 separation method Methods 0.000 claims description 4
- 230000009286 beneficial effect Effects 0.000 claims description 3
- 210000005069 ears Anatomy 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 241000209094 Oryza Species 0.000 claims 5
- 238000012258 culturing Methods 0.000 abstract description 3
- 240000007594 Oryza sativa Species 0.000 description 19
- 230000002349 favourable effect Effects 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 240000002582 Oryza sativa Indica Group Species 0.000 description 2
- 241000574138 Ozothamnus diosmifolius Species 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
-
- G—PHYSICS
- G10—MUSICAL INSTRUMENTS; ACOUSTICS
- G10L—SPEECH ANALYSIS OR SYNTHESIS; SPEECH RECOGNITION; SPEECH OR VOICE PROCESSING; SPEECH OR AUDIO CODING OR DECODING
- G10L21/00—Processing of the speech or voice signal to produce another audible or non-audible signal, e.g. visual or tactile, in order to modify its quality or its intelligibility
- G10L21/02—Speech enhancement, e.g. noise reduction or echo cancellation
Abstract
The invention discloses a cultivation method of high-quality fragrant long-grain japonica rice, which comprises the following steps: s1: parent selection is performed. S2: firstly, selecting two-line sterile line culture dwarf 64S with better affinity to japonica rice as female parent, and using national standard first-grade rice-flavor japonica rice variety Hubei-evening 17 as male parent to hybridize, thereby obtaining 52 grains of hybrid seeds. S3: f1 generations were planted with the seeds in step S2. And then the aromatic indica restorer line Xianghui 66 is used as a female parent, and F1 is used as a male parent for backcross to obtain 800 grains of triple-crossed seeds. Then planting three-way cross-bred F1, and using the japonica rice restorer R187 with long grain shape as a male parent to hybridize to obtain 600 grains of four-way cross-bred seeds. S4: the seeds in step S3 were selected and approximately 400 lines of four cross F1 were planted. S5: about 1000 strains of the four-cross variety F2 obtained in the step S4 were planted. S6: and (5) continuously and directionally culturing. The invention can solve the problem of incompatibility of indica-japonica hybrid.
Description
Technical Field
The invention relates to the technical field of rice breeding, in particular to a cultivation method of high-quality fragrant long-grain japonica rice.
Background
The prior fragrant rice in China is sourced from the first, the imported Qin fragrant rice belongs to the indica type, has good quality, but is expensive and cannot be consumed by ordinary people; and the rice is high, easy to lodging, poor in resistance, low in yield, easy to process and broken, and is not suitable for planting in China. Secondly, the domestic fragrant rice is mainly the Wuchang rice flower fragrant rice, belongs to the japonica type, has good quality, but has short grain shape, has serious glutinous rice property, is not popular with southern people, is originally produced in Heilongjiang province with high latitude in China, is not suitable for planting in other places, has low yield per mu, and cannot meet the requirements of consumers in China on high-grade fragrant rice.
Disclosure of Invention
The invention provides a cultivation method of high-quality fragrant long-grain japonica rice, which aims to solve the problems in the background art.
The invention provides a cultivation method of high-quality fragrant long-grain japonica rice, which comprises the following steps:
s1: parent selection is carried out, wherein the parent sources are Xianghui 66, Bei 64S, Hui-Niang 17 and R187, backbone parents are reasonably selected from the parents, the Xianghui 66 and the Hui-Niang 17 serve as the backbone parents, the two varieties serve as the backbone parents for hybridization, the typical distant hybridization mode of indica rice and japonica rice is achieved, the two varieties are matched, the good characters, the high quality, the fragrance and other favorable characters of the indica rice and japonica rice are guaranteed to have sufficient sources, and the short 64S and the R187 serve as auxiliary parents;
s2: firstly, selecting two-line sterile line with better affinity to japonica rice to cultivate dwarf 64S as female parent, using national standard first-grade rice fragrance type japonica rice variety Hubei-evening 17 as male parent to make hybridization, adopting manual glume cutting and emasculation hybridization method to obtain 52 grains of hybrid seeds.
S3: planting by using the hybrid seeds in the step S2, obtaining F1 plants, then using the fragrant medium indica restorer line Xianghui 66 as a female parent, using F1 as a male parent for backcrossing to obtain 800 triple-bred seeds, then planting triple-bred seeds F1, planting 600 single plants, selecting 6 plants with partial japonica rice plant types, partial long grain shapes and normal scattered powder as female parents when ears are drawn in the season, and then using the round-grained rice restorer line R187 with partial long grain shapes as a male parent for hybridization to obtain 600 quadruple-bred seeds in total;
s4: the seeds in step S3 were selected and approximately 400 lines of four cross F1 were planted. Selecting 50 excellent single plants with japonica rice type, long grain shape and high seed setting rate in the same season, selecting 10 single plants with better fragrance quality through indoor seed test, performing mixed threshing, and eliminating the rest plants.
S5: and (4) planting about 1000 four-cross plants F2 obtained in the step S4, selecting 100 excellent single plants with japonica type, long grain shape and high seed setting rate in the season, and selecting 20 pure plants with high quality and fragrance through indoor seed test. And then planting four-cross hybrid F3, inserting 20 strains, wherein 50 strains are planted in each strain, when the season is used, 4 strains with japonica type, long grain shape, high seed setting rate and small separation degree are selected, and 4 excellent single strains are selected in each strain.
S6: then planting the four-cross-bred F4 obtained in the step S5, inserting 16 strains, inserting 50 strains in each strain, selecting 4 excellent strains in the season, selecting 3 excellent strains in each strain, namely selecting 12 strains in total, continuously and directionally culturing by the method to obtain the four-cross-bred F8, selecting and breeding a new high-quality fragrant long-grain japonica rice strain W118 with a partial japonica plant type and a long grain shape, and then selecting and naming the strain as Exiang No. 2.
Preferably, the following components: in step S1, xianghui 66 is a typical long-grain high-quality aromatic indica type rice variety, hubei 17 is a typical round-grain high-quality aromatic japonica type rice variety, and bei 64S has a wide affinity characteristic, and is long-grain and excellent in quality, and R187 is a high-quality japonica rice variety with a long grain and a wide affinity characteristic.
Preferably: in the segregating generation, enough colonies are planted, and a plurality of excellent single plants which are slender in grain shape, japonica rice in plant shape, fragrant and good in quality are selected to be ensured until stable strains which meet the target are cultivated.
Preferably: in the steps, every generation needs to identify and retain the fragrance, so that the fragrance is not lost.
The cultivation method of the high-quality fragrant long-grain japonica rice has the beneficial effects that: the method comprises the steps of firstly, adopting indica-japonica hybridization, combining favorable characters of indica-japonica rice with favorable characters of japonica rice through directional cultivation and selection, simultaneously removing unfavorable characters of indica-japonica rice, secondly, adopting a wide-compatible variety as a medium, solving the problem of incompatibility of indica-japonica hybridization, thirdly, selecting a high-quality variety as a parent, improving the frequency of producing super-parent high-grade rice strains in filial generations, fourthly, introducing a fragrance gene, cultivating high-grade fragrant rice varieties, and fifthly, adopting a correct hybridization and double crossing method, and effectively improving the polymerization frequency of favorable characters.
Detailed Description
The invention is further illustrated by the following examples.
The invention provides a cultivation method of high-quality fragrant long-grain japonica rice, which comprises the following steps:
s1: selecting parents, wherein the parents are sourced from Xianghui 66, Bakui 64S, Hubei 17 and R187, and reasonably selecting backbone parents from the parents, wherein the Xianghui 66 and the Hubei 17 are used as the backbone parents, the two varieties are used as the backbone parents for hybridization, and belong to a typical distant hybridization mode of indica and japonica, the two varieties are matched, so that the good characters, high quality, fragrance and other beneficial characters of the indica and japonica are sufficiently sourced, and the Bakui 64S and the R187 are used as auxiliary parents;
s2: firstly, selecting two-line sterile line culture short 64S with better affinity to japonica rice as female parent, using national standard first-grade rice-flavor japonica rice variety Hubei-evening 17 as male parent for hybridization, and adopting manual glume cutting and castration hybridization method to obtain 52 hybrid seeds.
S3: planting by using the hybrid seeds in the step S2, obtaining F1 plants, then using the fragrant medium indica restorer line Xianghui 66 as a female parent, using F1 as a male parent for backcrossing to obtain 800 triple-bred seeds, then planting triple-bred seeds F1, planting 600 single plants, selecting 6 plants with partial japonica rice plant types, partial long grain shapes and normal scattered powder as female parents when ears are drawn in the season, and then using the round-grained rice restorer line R187 with partial long grain shapes as a male parent for hybridization to obtain 600 quadruple-bred seeds in total;
s4: the seeds from step S3 were selected and approximately 400 lines of four cross seed F1 were planted. Selecting 50 excellent single plants with japonica shape, long grain shape and high seed setting rate in the same season, selecting 10 single plants with better fragrance quality through indoor seed test, mixing, threshing, and eliminating the rest plants, thereby obtaining four-cross seeds F2;
s5: planting about 1000 four-cross-bred F2 obtained in the step S4, selecting 100 excellent single plants with better plant types, longer grain shapes and high maturing rate in the season, selecting 20 pure plants with better quality and fragrance through indoor seed test, thus obtaining four-cross-bred F3, then planting four-cross-bred F3, inserting 20 strains, 50 strains in each strain, selecting 4 strains with better plant types, longer grain shapes, high maturing rate and lower separation degree in the season, and selecting 4 excellent single plants in each strain, namely four-cross-bred F4;
s6: then planting the four-cross-bred F4 obtained in the step S5, inserting 16 strains, inserting 50 strains in each strain, selecting 4 excellent strains in the season, selecting 3 excellent strains in each strain, namely selecting 12 strains in total, continuously and directionally culturing by the method to obtain the four-cross-bred F8, selecting and breeding a new high-quality fragrant long-grain japonica rice strain W118 with a partial japonica plant type and a long grain shape, and then selecting and naming the strain as Exiang No. 2.
In step S1, xianghui 66 is a typical long-grain high-quality aromatic indica type rice variety, hubei 17 is a typical round-grain high-quality aromatic japonica type rice variety, and bei 64S has a wide affinity characteristic, and is long-grain and excellent in quality, and R187 is a high-quality japonica rice variety with a long grain and a wide affinity characteristic.
In the separation generation of the above steps, enough groups are planted to ensure that a plurality of excellent single plants with slender grain shape and japonica rice plant shape, fragrance and good quality are selected until stable strains meeting the target are cultivated.
In the steps, every generation needs to identify and retain the fragrance, so that the fragrance is not lost.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered as the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.
Claims (3)
1. A cultivation method of high-quality fragrant long-grain japonica rice is characterized by comprising the following steps:
s1: selecting parents, wherein the parents are sourced from Xianghui 66, Bakui 64S, Hubei 17 and R187, and reasonably selecting backbone parents from the parents, wherein the Xianghui 66 and the Hubei 17 are used as the backbone parents, the two varieties are used as the backbone parents for hybridization, and belong to a typical distant hybridization mode of indica and japonica, the two varieties are matched, so that the good characters, high quality, fragrance and other beneficial characters of the indica and japonica are sufficiently sourced, and the Bakui 64S and the R187 are used as auxiliary parents;
s2: firstly, selecting a two-line sterile line with better affinity to japonica rice to cultivate dwarf 64S as a female parent, hybridizing by using a national standard first-grade rice-flavor japonica rice variety Hubei-evening 17 as a male parent, and obtaining 52 hybrid seeds by adopting a manual glume cutting and emasculation hybridization method;
s3: planting by using the hybrid seeds in the step S2, obtaining F1 plants, then using the fragrant medium indica restorer line Xianghui 66 as a female parent, using F1 as a male parent for backcrossing to obtain 800 triple-bred seeds, then planting triple-bred seeds F1, planting 600 single plants, selecting 6 plants with partial japonica rice plant types, partial long grain shapes and normal scattered powder as female parents when ears are drawn in the season, and then using the round-grained rice restorer line R187 with partial long grain shapes as a male parent for hybridization to obtain 600 quadruple-bred seeds in total;
s4: selecting seeds in the step S3, planting 400 strains of four-cross-bred F1, selecting 50 excellent single strains with japonica shape, long grain shape and high seed setting rate in the season, selecting 10 single strains with better fragrance quality through indoor seed test, performing mixed threshing, and eliminating the rest;
s5: planting about 1000 four-cross-bred F2 obtained in the step S4, selecting 100 excellent single plants with japonica-like type, long grain shape and high maturing rate in season, selecting 20 pure fragrant plants with better quality through indoor seed test, then planting 20 strains of the four-cross-bred F3, inserting 50 strains in each strain, selecting 4 strains with japonica-like type, long grain shape, high maturing rate and low separation degree in season, and selecting 4 excellent single plants in each strain;
s6: then planting the four-cross variety F4 obtained in the step S5, inserting 16 strains, wherein 50 strains are planted in each strain, 4 excellent strains are selected in the season, 3 excellent strains are selected in each strain, namely 12 strains are selected together, and the four-cross variety F8 is obtained by continuous directional cultivation by the method, so that a new high-quality fragrant long-grain japonica rice strain with a japonica-like plant type and a long grain shape is bred;
in step S1, xianghui 66 is a typical long-grain high-quality aromatic indica type rice variety, hubei 17 is a typical round-grain high-quality aromatic japonica type rice variety, and bei 64S has a wide affinity characteristic, and is long-grain and excellent in quality, and R187 is a high-quality japonica rice variety with a long grain and a wide affinity characteristic.
2. The method for cultivating high-quality aromatic long-grained nonglutinous rice according to claim 1, wherein the method comprises the following steps: in the segregating generation, enough groups are planted, and a plurality of excellent single plants with slender grain shapes, japonica rice plant shapes, fragrance and good quality are selected to be ensured until stable strains meeting the target are cultivated.
3. The method for cultivating the high-quality aromatic long-grained nonglutinous rice according to claim 1, wherein the method comprises the following steps: in the steps, the fragrance needs to be identified and kept every generation, so that the fragrance is not lost.
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| CN201910213060.4A CN109757359B (en) | 2019-03-20 | 2019-03-20 | Method for cultivating high-quality fragrant long-grain japonica rice |
| PCT/CN2019/111557 WO2020186740A1 (en) | 2019-03-20 | 2019-10-17 | Real-time voice noise reduction system applied to ground-to-air communication |
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| CN111567397A (en) * | 2020-05-20 | 2020-08-25 | 江苏（武进）水稻研究所 | High-quality long-grain fragrance type japonica rice Wujing 9075 and breeding method |
| CN111567400A (en) * | 2020-06-29 | 2020-08-25 | 湖北省农业科学院粮食作物研究所 | Method for efficiently breeding high-yield high-quality fragrant long-grain japonica rice |
| CN111727877B (en) * | 2020-07-29 | 2023-03-31 | 重庆市农业科学院 | Method for cultivating high-quality dual-purpose genic male sterile line of fragrant rice |
| CN111869562B (en) * | 2020-08-14 | 2022-11-22 | 江苏（武进）水稻研究所 | Breeding method of high-quality small-grain-flavor japonica rice Wuxiangjing 113 |
| CN113131965B (en) * | 2021-04-16 | 2023-11-07 | 成都天奥信息科技有限公司 | Civil aviation very high frequency ground-air communication radio station remote control device and voice discrimination method |
| CN113796306B (en) * | 2021-09-13 | 2022-09-16 | 江苏丘陵地区镇江农业科学研究所 | Breeding method for cultivating Huang-Huai-japonica rice variety by utilizing cold region rice germplasm resources |
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| JP5382745B2 (en) * | 2009-07-08 | 2014-01-08 | 株式会社日立製作所 | Noise removing apparatus and noise removing method |
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