Summary of the invention
The object of the present invention is to provide the selection of a kind of erect head long stigma long fringe neck CMS line, thus improve outcrossing seed-setting rate and the hybrid seed yield of CMS line.The present invention selects erect head rice varieties Shennong-265, B(Chen Yu is new in long stigma rice varieties sea. the election effects of hybrid rice long stigma male sterile line-Hai A. and the Journal of Hainan University's natural science edition, 1991, 9 (2): 54-58), long fringe neck rice varieties IR68902eB1 provides parent for objective trait, be recurrent parent to produce the larger excellence of upper popularizing area three be maintenance, by hybridization, reestablish diplomatic relations and backcross transformation, be bred as economical character, yield traits is close with former male sterile line, there is erect head simultaneously, the CMS line of long stigma and long fringe neck proterties, this male sterile line not Bao Sui, stigma exposing ratio is high, outcrossing seed-setting rate is high, the hybrid seed yield of hybrid rice can be improved with its preparation ternary hybrid rice, reduce production cost and reduce environmental pollution.
Technical scheme of the present invention is as follows:
1) select erect head rice varieties Shennong-265 (A), long stigma rice varieties sea B(B), long fringe neck rice varieties IR68902eB1(C) provide parent for objective trait, it be maintenance is recurrent parent (R) that China produces the larger excellence of popularizing area three at present, make hybridization A/B, must F be hybridized
1in generation, with F
1-ABrepresent;
2) plantation hybridization F
1in generation, make hybridization F in flowering stage
1-AB/ C, must reestablish diplomatic relations F
1, with F
1-ABCrepresent;
3) F is planted
1-ABC, selfing sowing obtains F
2-ABC, plantation F
2-ABC, colony 1800-2000 strain, full heading time screening has the plant listing mark of erect head, long stigma and long fringe neck proterties simultaneously, and the maturing stage is individual plant sowing (F respectively
3-ABC);
4) strain plantation 3) there is while gained the F of erect head, long stigma and long fringe neck proterties
3-ABC, each strain plants 30 strains, full heading time screening have simultaneously erect head, long stigma with long fringe neck proterties and proterties without the strain 2-3 is separated, therefrom select individual plant 3 strain of economical character excellence, be maternal with recurrent parent (R), work hybridizes R/F
3-ABC, must F be hybridized
1in generation, with F
1-ABCRrepresent;
5) 4 are planted) gained F
1-ABCR, maturing stage mixing sowing obtains F
2-ABCR, plantation F
2-ABCR, colony 1400-1600 strain, full heading time screening has the individual plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom select individual plant 2 strain of economical character excellence, backcross R/F
2-ABCR, must backcross F
1in generation, with BC
1f
1represent;
6) BC that backcrosses is planted
1f
1, selfing obtains BC
1f
2, plantation BC
1f
2, full heading time screening has the plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom selects economical character individual plant 2 strain the most similar to recurrent parent R and recurrent parent R(to make female parent) continue to backcross, must backcross BC
2f
1generation;
7) BC that backcrosses is planted
2f
1in generation, selfing obtains BC
2f
2, plantation BC
2f
2, full heading time screening has the plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom selects economical character individual plant 2 strain the most similar to recurrent parent R and recurrent parent R(to make female parent) continue to backcross, must backcross BC
3f
1generation;
8) step 7) is repeated until BC
4f
1generation, plantation BC
4f
1, selfing obtains BC
4f
2, plantation BC
4f
2, full heading time screening has the plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom select the economical character individual plant 1-2 strain the most similar to recurrent parent R to shape namely to be bred as the long fringe neck three of erect head long stigma be maintainer, abbreviation ZCeB system;
9) ZCeB system and three is that the corresponding CMS line (making female parent) of maintainer recurrent parent R is hybridized and 3 generations that backcrossed, and is namely bred as erect head long stigma long fringe neck CMS line, is called for short ZCeA system;
The selection of erect head long stigma long fringe neck CMS line, its recurrent parent three is maintenance is Long Tepu B, flower 2B or Fujian mark 1B, and its corresponding CMS line is Long Tepu A, flower 2A or Fujian mark 1A.
beneficial effect of the present invention:
Effect of the present invention is by hybridization and back cross breeding method controlling the channel genes of erect head, long stigma and long fringe neck proterties in the CMS line of hybrid rice, be bred as the CMS line with erect head, long stigma and long fringe neck proterties, this male sterile line not bag neck, stigma exposing ratio is high, outcrossing seed-setting rate is high, with its preparation ternary hybrid rice can improve hybrid rice hybrid seed yield, reduce production cost and reduce environmental pollution.Seed farm every mu can increase yield 8-12%, reduces and sprays gibberellin cost 60-80 unit, increases economic benefit 150-220 unit.
embodiment:
Embodiment 1:
1) select erect head rice varieties Shennong-265 (A), long stigma rice varieties sea B(B), long fringe neck rice varieties IR68902eB1(C) provide parent, Long Tepu B recurrent parent (R) for objective trait, make hybridization A/B, must F be hybridized
1in generation, with F
1-ABrepresent;
2) plantation hybridization F
1in generation, make hybridization F in flowering stage
1-AB/ C, must reestablish diplomatic relations F
1, with F
1-ABCrepresent;
3) F is planted
1-ABC, selfing sowing obtains F
2-ABC, plantation F
2-ABC, colony 1800 strain, full heading time screening has the plant listing mark of erect head, long stigma and long fringe neck proterties simultaneously, and the maturing stage is individual plant sowing (F respectively
3-ABC);
4) strain plantation 3) there is while gained the F of erect head, long stigma and long fringe neck proterties
3-ABC, each strain plants 30 strains, full heading time screening have simultaneously erect head, long stigma with long fringe neck proterties and proterties without the strain is separated 2, therefrom select individual plant 3 strain of economical character excellence, be maternal with recurrent parent (R), work hybridizes R/F
3-ABC, must F be hybridized
1in generation, with F
1-ABCRrepresent;
5) 4 are planted) gained F
1-ABCR, maturing stage mixing sowing obtains F
2-ABCR, plantation F
2-ABCR, colony 1400 strain, full heading time screening has the individual plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom select individual plant 2 strain of economical character excellence, backcross R/F
2-ABCR, must backcross F
1in generation, with BC
1f
1represent;
6) BC that backcrosses is planted
1f
1, selfing obtains BC
1f
2, plantation BC
1f
2, full heading time screening has the plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom selects economical character individual plant 2 strain the most similar to recurrent parent R and recurrent parent R(to make female parent) continue to backcross, must backcross BC
2f
1generation;
7) BC that backcrosses is planted
2f
1in generation, selfing obtains BC
2f
2, plantation BC
2f
2, full heading time screening has the plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom selects economical character individual plant 2 strain the most similar to recurrent parent R and recurrent parent R(to make female parent) continue to backcross, must backcross BC
3f
1generation;
8) step 7) is repeated until BC
4f
1generation, plantation BC
4f
1, selfing obtains BC
4f
2, plantation BC
4f
2full heading time screening has the plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom select the economical character individual plant 1-2 strain the most similar to recurrent parent R to shape namely to be bred as the long fringe neck three of erect head long stigma be maintainer (being called for short ZC Long Te Pu eB);
9) " ZC Long Te Pu eB " and Long Tepu A(make female parent) hybridize and 3 generations that backcrossed, be namely bred as erect head long stigma long fringe neck CMS line (being called for short ZC Long Te Pu eA);
" ZC Long Te Pu eA " has erect head, long stigma and long fringe neck proterties, " ZC Long Te Pu eA " not bag neck, stigma exposing ratio is high, outcrossing seed-setting rate is high, can increase yield 8% with its preparation ternary hybrid rice seed farm every mu, reduce and spray gibberellin cost 60 yuan, increase economic benefit 150 yuan.
Embodiment 2:
1) select erect head rice varieties Shennong-265 (A), long stigma rice varieties sea B(B), long fringe neck rice varieties IR68902eB1(C) provide parent for objective trait, flower 2B is recurrent parent (R), makes hybridization A/B, must hybridize F
1in generation, with F
1-ABrepresent;
2) plantation hybridization F
1in generation, make hybridization F in flowering stage
1-AB/ C, must reestablish diplomatic relations F
1, with F
1-ABCrepresent;
3) F is planted
1-ABC, selfing sowing obtains F
2-ABC, plantation F
2-ABC, colony 1900 strain, full heading time screening has the plant listing mark of erect head, long stigma and long fringe neck proterties simultaneously, and the maturing stage is individual plant sowing (F respectively
3-ABC);
4) strain plantation 3) there is while gained the F of erect head, long stigma and long fringe neck proterties
3-ABC, each strain plants 30 strains, full heading time screening have simultaneously erect head, long stigma with long fringe neck proterties and proterties without the strain is separated 3, therefrom select individual plant 3 strain of economical character excellence, be maternal with recurrent parent (R), work hybridizes R/F
3-ABC, must F be hybridized
1in generation, with F
1-ABCRrepresent;
5) 4 are planted) gained F
1-ABCR, maturing stage mixing sowing obtains F
2-ABCR, plantation F
2-ABCR, colony 1500 strain, full heading time screening has the individual plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom select individual plant 2 strain of economical character excellence, backcross R/F
2-ABCR, must backcross F
1in generation, with BC
1f
1represent;
6) BC that backcrosses is planted
1f
1, selfing obtains BC
1f
2, plantation BC
1f
2, full heading time screening has the plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom selects economical character individual plant 2 strain the most similar to recurrent parent R and recurrent parent R(to make female parent) continue to backcross, must backcross BC
2f
1generation;
7) BC that backcrosses is planted
2f
1in generation, selfing obtains BC
2f
2, plantation BC
2f
2, full heading time screening has the plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom selects economical character individual plant 2 strain the most similar to recurrent parent R and recurrent parent R(to make female parent) continue to backcross, must backcross BC
3f
1generation;
8) step 7) is repeated until BC
4f
1generation, plantation BC
4f
1, selfing obtains BC
4f
2, plantation BC
4f
2, full heading time screening has the plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom select the economical character individual plant 1-2 strain the most similar to recurrent parent R to shape namely to be bred as the long fringe neck three of erect head long stigma be maintainer (being called for short the colored 2eB system of ZC);
9) " ZC flower 2eB " makes female parent with flower 2A() hybridize and 3 generations that backcrossed, be namely bred as erect head long stigma long fringe neck CMS line (being called for short ZC flower 2eA);
" ZC flower 2eA " has erect head, long stigma and long fringe neck proterties, " ZC flower 2eA " not bag neck, stigma exposing ratio is high, outcrossing seed-setting rate is high, can increase yield 10% with its preparation ternary hybrid rice seed farm every mu, reduce and spray gibberellin cost 70 yuan, increase economic benefit 190 yuan.
Embodiment 3:
1) select erect head rice varieties Shennong-265 (A), long stigma rice varieties sea B(B), long fringe neck rice varieties IR68902eB1(C) provide parent for objective trait, Fujian mark 1B is recurrent parent (R), makes hybridization A/B, must hybridize F
1in generation, with F
1-ABrepresent;
2) plantation hybridization F
1in generation, make hybridization F in flowering stage
1-AB/ C, must reestablish diplomatic relations F
1, with F
1-ABCrepresent;
3) F is planted
1-ABC, selfing sowing obtains F
2-ABC, plantation F
2-ABC, colony 2000 strain, full heading time screening has the plant listing mark of erect head, long stigma and long fringe neck proterties simultaneously, and the maturing stage is individual plant sowing (F respectively
3-ABC);
4) strain plantation 3) there is while gained the F of erect head, long stigma and long fringe neck proterties
3-ABC, each strain plants 30 strains, full heading time screening have simultaneously erect head, long stigma with long fringe neck proterties and proterties without the strain is separated 3, therefrom select individual plant 3 strain of economical character excellence, be maternal with recurrent parent (R), work hybridizes R/F
3-ABC, must F be hybridized
1in generation, with F
1-ABCRrepresent;
5) 4 are planted) gained F
1-ABCR, maturing stage mixing sowing obtains F
2-ABCR, plantation F
2-ABCR, colony 1600 strain, full heading time screening has the individual plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom select individual plant 2 strain of economical character excellence, backcross R/F
2-ABCR, must backcross F
1in generation, with BC
1f
1represent;
6) BC that backcrosses is planted
1f
1, selfing obtains BC
1f
2, plantation BC
1f
2, full heading time screening has the plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom selects economical character individual plant 2 strain the most similar to recurrent parent R and recurrent parent R(to make female parent) continue to backcross, must backcross BC
2f
1generation;
7) BC that backcrosses is planted
2f
1in generation, selfing obtains BC
2f
2, plantation BC
2f
2, full heading time screening has the plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom selects economical character individual plant 2 strain the most similar to recurrent parent R and recurrent parent R(to make female parent) continue to backcross, must backcross BC
3f
1generation;
8) step 7) is repeated until BC
4f
1generation, plantation BC
4f
1, selfing obtains BC
4f
2, plantation BC
4f
2full heading time screening has the plant of erect head, long stigma and long fringe neck proterties simultaneously, and therefrom select the economical character individual plant 1-2 strain the most similar to recurrent parent R to shape namely to be bred as the long fringe neck three of erect head long stigma be maintainer (be called for short ZC Fujian and mark 1eB system);
9) " ZC Fujian mark 1eB " marks 1A(with Fujian and makes female parent) hybridize and 3 generations that backcrossed, be namely bred as erect head long stigma long fringe neck CMS line (being called for short ZC Fujian mark 1eA);
" ZC Fujian mark 1eA " has erect head, long stigma and long fringe neck proterties, " ZC Fujian mark 1eA " not bag neck, stigma exposing ratio is high, outcrossing seed-setting rate is high, can increase yield 12% with its preparation ternary hybrid rice seed farm every mu, reduce and spray gibberellin cost 80 yuan, increase economic benefit 220 yuan.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.