CN109089873A - Obtain the method that can be used for the Parents In Hybrid Rice combination of the same period live streaming production of hybrid seeds - Google Patents
Obtain the method that can be used for the Parents In Hybrid Rice combination of the same period live streaming production of hybrid seeds Download PDFInfo
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- CN109089873A CN109089873A CN201810878850.XA CN201810878850A CN109089873A CN 109089873 A CN109089873 A CN 109089873A CN 201810878850 A CN201810878850 A CN 201810878850A CN 109089873 A CN109089873 A CN 109089873A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention relates to a kind of methods that acquisition can be used for the Parents In Hybrid Rice combination of the same period live streaming production of hybrid seeds, comprising the following steps: and the sterile line male parent and sterile line that are used to cultivate sterile line is maternal, hybridized to obtain F1 generation；It is selfed to obtain the F2 generation of trait segregation with F1 generation；It is divided into multiple sterile line parental autocopulations group from the good strain of selection traits in one or many self progenies in F2 generation or F2 generation according to its duration from seeding to heading on production of hybrid seeds ground；According to the one or more corresponding restorer strains of the duration from seeding to heading of each sterile line parental autocopulation group selection, as restorer undetermined；The strain of sterile line parental autocopulation group carries out one or many selfings, and according to the character determination of corresponding restorer undetermined, and rejects separation strains, obtains the stable sterile line of character；By strain and the corresponding restorer test cross undetermined in the stable sterile line of character, the high combination of coordinate force is sterile line and the restorer combination for breeding of hybrid rice.
Description
Technical field
The present invention relates to breeding of hybridized rice and production of hybrid seeds field, more specifically it relates to which a kind of acquisition can be used for same period live streaming
The method of the Parents In Hybrid Rice combination of the production of hybrid seeds, and the Parents In Hybrid Rice combination obtained using this method carry out same period live streaming
The method of the production of hybrid seeds.
Background technique
Rice is the staple food crop of China, at present about 4.3~4.4 hundred million mu of rice annual planting area, wherein hybridizing water
Rice annual planting area is about 2.2 hundred million mu, accounts for 50% or more of total rice area, is significantly increased production and grain security for rice in China
It makes a great contribution.
The principle of hybrid rice is: selecting two genetically has different, while their merit again can be mutually
The rice varieties of benefit, are hybridized, and obtained first generation cenospecies has hybrid vigour.First generation cenospecies is used for agricultural kind
Plant can all obtain good yield in terms of resistance and yield.The seed that hybrid rice is selfed cannot maintain its hybrid excellent
Gesture, therefore the hybrid rice seeds for agricultural production that common peasant household obtains must all be obtained by the hybridization of two parents
, which is known as the production of hybrid seeds.
The production of hybrid seeds of hybrid rice is one of the link of agricultural production most critical.Male parent and maternal flower synchronization are to guarantee the production of hybrid seeds
The premise of good quality and high output, however, this is also the important limiting factor of the production of hybrid seeds.Since male parent and female parent belong to different strains,
Florescence is not often identical, it is however generally that, the florescence of male parent is short and concentrates, and the maternal florescence is often long and disperses, and Parent
Ear flowering time often poor 7-15d.Therefore, it in order to make flower synchronization, generally requires in planting process according to Parent group
The florescence feature of conjunction is adjusted, including determines sowing gap stage, prediction florescence, adjusts vegetative growth phase or reproduction by water and fertilizer management
Growth period etc..
However, these methods on the one hand greatly increase the workload of seeding farmer, on the other hand also it is under one's control be because
Element (such as broadcasting difference design is made mistakes, prediction of flowering period is inaccurate, Parent rice shoot is excessive or very few, field management is improper etc.) and impersonal force
The influence (such as weather, pest and disease damage, soil moisture content etc.) of controllable factor.Therefore, it is necessary to a kind of new Parent combined methods
And producing method for seed.
Summary of the invention
Inventor in the course of the research it was unexpectedly found that, use the son being selfed after the hybridization of two plants of photo-thermo-sensitive genic male sterile lines
Trait segregation, F2 generation and its one or many selfings can occur in three indexs in duration from seeding to heading, florescent and flower for generation
The range when duration from seeding to heading of offspring, florescent and flower has exceeded the character range of two parents.Due to the active energy of column cap
It is enough to maintain a more long than pollen, so, the florescence of restorer (being no more than 2 days) slightly more late than sterile line will not influence the production of hybrid rice
Amount.In addition, inventor also found, on the flowering time in one day, when sterile line first bloom expose column cap as far as possible after restore
System, which reblooms, to be conducive to improve pollination rate, to improve hybrid rice seeds yield.
Based on the above discovery, the present invention provides the Parents In Hybrid Rice combinations that a kind of acquisition can be used for the same period live streaming production of hybrid seeds
Method, comprising the following steps:
S1: the sterile line male parent and sterile line that are used to cultivate sterile line is maternal, hybridized to obtain F1 generation；
S2: it is selfed to obtain the F2 generation of trait segregation with F1 generation；
S3: from the good strain of selection traits in one or many self progenies in F2 generation or F2 generation, according to it in the production of hybrid seeds
The duration from seeding to heading on ground is divided into multiple sterile line parental autocopulations group；
S4: according to the one or more corresponding restorer strains of the duration from seeding to heading of each sterile line parental autocopulation group selection
System, as restorer undetermined；
S5: the strain of sterile line parental autocopulation group carries out one or many selfings, and according to corresponding undetermined extensive
The character determination for being again, and separation strains are rejected, obtain the stable sterile line of character；
S6: by strain and corresponding restorer test cross undetermined, the high group of coordinate force in the stable sterile line of the character
Closing is sterile line and the restorer combination for being used for breeding of hybrid rice.
In a preferred embodiment, the sterile line male parent and sterile line female parent are respectively to have different prepotencies
Photoperiod-temperature sensitive genie male-sterile line strain.
In a specific embodiment, the prepotency of the sterile line male parent includes having brown planthopper resistant gene, institute
The prepotency for stating sterile line female parent includes with blast resistant gene.
In a specific embodiment, the sterile line male parent is 043S, and the sterile line female parent is 8109S.
In a preferred embodiment, selected in S3 F2 generation in strain as sterile line parental autocopulation group at
The standard of member includes: that the bilateral exposing ratio of column cap is high, it is big to open clever angle, it is good to close grain husk, and has polymerize the sterile line male parent and not
Educating is maternal prepotency.
In a preferred embodiment, in S5, the self progeny of sterile line parental autocopulation group strain is carried out
The standard of selection includes: that duration from seeding to heading is in the production of hybrid seeds identical as corresponding restorer undetermined or early no more than 2 days, is being made when spending
Kind ground is 10-40 minutes more early than corresponding restorer undetermined, and florescent is 7-10 days.
It in a preferred embodiment, further include that the stable sterile line of the character is continued to purifying to obtain after S6
The step of being sheerly sterile line.
This method passes through using the F2 having been surprisingly found that for duration from seeding to heading segregation phenomenon, can obtain in a collection of breeding process more
A duration from seeding to heading is identical or different, can be used for substantially increasing with the sterile line that existing some restorers combine in the market
The success rate of success rate of breeding and Parent combination.Obtained hybrid rice Parent combination can carry out same during the production of hybrid seeds
Phase sowing, flower synchronization is good, and the more existing hybrid rice Parent combination production of hybrid seeds of seed production is substantially improved, and both saves
It is artificial, and improve yield.
The present invention also provides the hybrid rice seed breeding process of live streaming of same period parent a kind of comprising obtains the above method
Sterile line and the restorer group indentured period be seeded in the step in seed farm.
In one embodiment, the producing method for seed the following steps are included:
1) sterile line and restorer seed are sowed simultaneously；
2) it when blooming, is pollinated with the restorer to the sterile line；
3) the solid obtained seed of the sterile line is hybrid rice seeds.
Unless otherwise defined, otherwise all technologies used herein and scientific term all have with it is of the art
Those of ordinary skill usually understands identical meaning.
" duration from seeding to heading " means the number of days that since rice ear being seeded into.
" florescent " refers to that, from flower first time open, female, stamen is exposed from perianth, all withered to flower,
All stamens or the deactivated number of days of gynoecium.
" when flower " refers to duration of flowering, the time of daily flowering.
" restorer " means filial generation can be made to restore male-fertile feature after a certain strain hybridizes with sterile line.
" selfing " mean the female and male gametophyte from same individual combination or between identical genotype individuals mating or
Mating between individual from same clone.
" test cross ", on rice, test cross refers to sterile line as female parent, hybridizes from different restorers, measures the cooperation of parent
Power, the cross combination for selecting F1 generation advantage strong.
" production of hybrid seeds " refer to that the parent of plantation hybrid rice makes it be hybridized to obtain hybrid rice seeds location, or
With place similar in the place weather hydrology.
Detailed description of the invention
Fig. 1 is one and cultivates the flow chart that can be used for the Parents In Hybrid Rice combination that the production of hybrid seeds is broadcast live in the same period.
Specific embodiment
The principle and features of the present invention will be described below with reference to the accompanying drawings, and the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the invention.
1. the male parent of sterile line and maternal selection
Selection light (temperature) quick line with genic sterile 043S does male parent, and 8109S does female parent.043S flowering habit is good, short bar, broadcasts the beginning and goes through
Phase 95 days, florescent 10 days, 9:50a.m.-11:40a.m when spending carried brown planthopper resistant gene Bph14 and Bph15.8109S
Duration from seeding to heading 92 days, florescent 12 days, 10:00a.m.-12:00 when spending carried blast resistant gene Pi1, Pi2.
2. the breeding process of sterile line
Hybridized to obtain F1 generation with 043S with 8109S, plant F1 in Hainan, self-fertility obtains F2, collects whole F2 generation kinds
Son.
On May 10th, 2010 Hubei Wuhan plantation F2 generation 10000 plants, statistics duration from seeding to heading it was unexpectedly found that, F2
Huge difference is presented compared with F1 generation and 043S and 8109S in the duration from seeding to heading of each strain, and duration from seeding to heading was from strain in 70-100 days
System is all distributed.Hua Shizao is therefrom selected, concentration of blooming, the bilateral exposing ratio of column cap is high, closes good 1366 plants of sterile single plant of grain husk, presses
Duration from seeding to heading grouping.October, band rice stub obtained F3 to Hainan bagging selfing breeding.The beginning is broadcast according to the 1366 of selection plants of F2 strains
Issue evidence is gone through, selects some restorer strains as restorer undetermined.
On May 12nd, 2011 is sowed in Hubei Wuhan, plants F3 by strain, each F3 strain plants 100 plants, and tillering stage takes
Blade Markers for Detection Bph14, Bph15, Pi1, Pi2, late July start to ear, microscopy pollen fertility.The same period plantation to
Restorer is determined, when counting the duration from seeding to heading, florescent and flower of each restorer.Screening polymerization Bph14, Bph15, Pi1, Pi2
Gene, pollen sterility degree 100%, economical character good compared with neat and flowering habit (duration from seeding to heading and corresponding recovery undetermined
Be it is identical or it is early be no more than 2 days, florescent 7-10 days, when flower, was 10-40 minutes more early than corresponding restorer undetermined, and the same day opens
Flower the same day close grain husk) totally 1216 plants of single plant, October band rice stub to Hainan breeding, obtain F4.
On May 10th, 2012, the sowing of Hubei Wuhan plant F4 by strain, and each strain plants 80 plants.By F3 method again
Carry out one wheel screening purifying, screening polymerization Bph14, Bph15, Pi1, Pi2 gene, pollen sterility degree 100%, economical character compared with
Neat and flowering habit is good, and (duration from seeding to heading is identical as corresponding restorer undetermined or early no more than 2 days, florescent 7-10
It, when flower, is 10-40 minute more early than corresponding restorer undetermined, the same day bloom on the day of close grain husk) strain, removal separation strains.October
F5 is obtained to Hainan breeding by strain band rice stub, heredity is gradually stable at this time, can be used for carrying out test cross.
In March, 2013, Hainan Lingshui are largely measured with hot water emasculation method with corresponding restorer undetermined, and summer is in lake
Observe hybrid vigour in northern Wuhan.
Screening purifying is continued in positive season in 2013, Hubei Wuhan, screens critical temperature of sterility, selection infertility with low temperature cold-penetration pond
It is numerous for further purifying expansion to retain rice stub in 23.5 degrees Celsius of single plants below for origin temp.The same year and restorer system
Kind, observe heterosis, hybrid vigor.
Critical temperature of sterility is screened with cold-penetration pond in positive season in 2014, Hubei Wuhan, retains critical temperature of sterility and takes the photograph 23.5
Family name's degree single plant below is as original seed.
By above method, obtain that multiple duration from seeding to heading are close and the higher sterile line of coordinate force/restorer combination.
3. breeding of hybrid rice
Easy S/ China is chosen from multiple combinations that above method screens accounts for combination progress production of hybrid seeds test.Easy S be by with
The sterile line that upper method is cultivated, the strain be photo-thermo-sensitive genic male sterile line, duration from seeding to heading Hubei be 79 days, florescent 8 days,
9:00a.m.-11:30a.m, plant height 78cm or so when flower, loose moderate, leaf color is light green, sword-like leave, upright, and tillering ability is strong, Bao Jing
Degree is small, and fringe layer is neat, and flowering habit is good, Hua Shizao, concentration of blooming, and column cap is big, colourless and double exposing ratios are high, opens clever angle
Greatly, it is good to close grain husk, outcrossing seed-setting rate is high, polymerize brown planthopper resistant gene Bph14, Bph15 and blast resistant gene Pi1, Pi2.China accounts for
It is common restorer, duration from seeding to heading 81 days, florescent 8 days, 9:30a.m.-11:30a.m when flower.The duration from seeding to heading ratio of easy S
China accounts for 2 days short, and florescent is identical, it is contemplated that being capable of preferably flower synchronization.
It is accounted for easy S and China in the Luotian County of Hubei production of hybrid seeds within 2016,0.5 mu of area, two phase male parents, a phase male parent compares maternal early by 7
Its sowing is sowed, rice transplantation by hand for the second stage of male parent 10 days slower than a phase male parent, sowing on maternal May 22, maternal August neat fringe on the 12nd,
Maternal florescence and the second stage of male parent are met preferably, and 406 jin of hybrid seed per mu yield.
The Hubei province production of hybrid seeds in 2017, father, maternal in same period May 20 live streaming (male parent drilling), male parent only broadcasts a phase,
Father, female flower synchronization are preferable, maternal July 30 neat fringe, 432 jin of hybrid seed per mu yield.
4. hybrid rice plants example
The Ezhou of Hubei in 2017, easy S with China account for easy two excellent China hybridize account in May 9 sowing, July 25 beginning fringe,
109 centimetres of plant height, the effective fringe 17.55 ten thousand of mu, 25.4 centimetres of spike length, number of grain per ear 206, setting percentage 81.68%, mass of 1000 kernel
23.27 grams, 687 kilograms of yield.Concurrent control kind is rich two excellent No. 4, August beginning fringe on the 2nd, 143.2 centimetres of plant height, the effective fringe of mu
13.95 ten thousand, 27.7 centimetres of spike length, number of grain per ear 190, setting percentage 81.36%, 29.37 grams of mass of 1000 kernel, 631.2 kilograms of yield.
Easy two excellent Hua Zhanyi passes through ripe late group of regional testing of Xian in the 2017 Nian Guo Dao Keqi association middle and lower reach of Yangtze River.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (9)
1. a kind of acquisition can be used for the method for the Parents In Hybrid Rice combination of the same period live streaming production of hybrid seeds, which is characterized in that including following
Step:
S1: the sterile line male parent and sterile line that are used to cultivate sterile line is maternal, hybridized to obtain F1 generation；
S2: it is selfed to obtain the F2 generation of trait segregation with F1 generation；
S3: from the good strain of selection traits in one or many self progenies in F2 generation or F2 generation, according to it on production of hybrid seeds ground
Duration from seeding to heading is divided into multiple sterile line parental autocopulations group；
S4: according to the one or more corresponding restorer strains of the duration from seeding to heading of each sterile line parental autocopulation group selection, make
For restorer undetermined；
S5: the strain of sterile line parental autocopulation group carries out one or many selfings, and according to corresponding restorer undetermined
Character determination, and reject separation strains, obtain the stable sterile line of character；
S6: by strain and the corresponding restorer test cross undetermined in the stable sterile line of the character, the high combination of coordinate force is
For for breeding of hybrid rice sterile line and restorer combination.
2. the method according to claim 1, wherein the sterile line male parent and sterile line female parent are respectively to have
The photoperiod-temperature sensitive genie male-sterile line strain of different prepotencies.
3. according to the method described in claim 2, it is characterized in that, the prepotency of the sterile line male parent includes brown with resisting
Plant hopper gene, the prepotency of the sterile line female parent include having blast resistant gene.
4. according to the method described in claim 3, the sterile line is maternal it is characterized in that, the sterile line male parent is 043S
For 8109S.
5. according to the method described in claim 2, it is characterized in that, selecting the strain in F2 generation as the sterile line parent in S3
The standard of this inbreeding population member includes: that the bilateral exposing ratio of column cap is high, it is big to open clever angle, it is good to close grain husk, and polymerize it is described not
Educate be male parent and sterile line female parent prepotency.
6. the method according to claim 1, wherein in S5, to sterile line parental autocopulation group strain
The standard that self progeny carries out selection includes: that duration from seeding to heading is in the production of hybrid seeds identical as corresponding restorer undetermined or morning is no more than
2 days, in the production of hybrid seeds 10-40 minutes more early than corresponding restorer undetermined when spending, florescent was 7-10 days.
7. method according to claim 1 to 6, which is characterized in that further include that the character is steady after S6
Fixed sterile line continues the step of purifying obtains pure lines sterile line.
8. the hybrid rice seed breeding process of same period live streaming parent a kind of, which is characterized in that including by any one of claim 1-7
The sterile line and restorer group indentured period that the method obtains are planted in the step in seed farm.
9. hybrid rice seed breeding process according to claim 8, which comprises the following steps:
1) sterile line and restorer seed are sowed simultaneously；
2) it when blooming, is pollinated with the restorer to the sterile line；
3) the solid obtained seed of the sterile line is hybrid rice seeds.
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| CN1052018A (en) * | 1990-11-16 | 1991-06-12 | 四川省万县地区农业科学研究所 | The regeneration producing method for seed of hybrid rice |
| CN1561692A (en) * | 2004-04-20 | 2005-01-12 | 河北省农垦科学研究所 | Method for preparing bilinear hybrid rice seed using ecological male sterile |
| CN103493726A (en) * | 2013-10-21 | 2014-01-08 | 安徽省农业科学院水稻研究所 | Method for mechanized seed production of hybrid rice through integration of seedling slinging technology and direct seeding technology |
| CN105104168A (en) * | 2015-08-07 | 2015-12-02 | 安徽省农业科学院水稻研究所 | Hybrid rice breeding method using parents with close duration from seeding to heading |
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2018
- 2018-08-03 CN CN201810878850.XA patent/CN109089873B/en active Active
Patent Citations (4)
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|---|---|---|---|---|
| CN1052018A (en) * | 1990-11-16 | 1991-06-12 | 四川省万县地区农业科学研究所 | The regeneration producing method for seed of hybrid rice |
| CN1561692A (en) * | 2004-04-20 | 2005-01-12 | 河北省农垦科学研究所 | Method for preparing bilinear hybrid rice seed using ecological male sterile |
| CN103493726A (en) * | 2013-10-21 | 2014-01-08 | 安徽省农业科学院水稻研究所 | Method for mechanized seed production of hybrid rice through integration of seedling slinging technology and direct seeding technology |
| CN105104168A (en) * | 2015-08-07 | 2015-12-02 | 安徽省农业科学院水稻研究所 | Hybrid rice breeding method using parents with close duration from seeding to heading |
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| FENGFENG FAN等: "Development of Elite BPH-Resistant Wide-Spectrum Restorer Lines for Three and Two Line Hybrid Rice", 《FRONTIERS IN PLANT SCIENCE》 * |
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