CN1042088C

CN1042088C - Method of selection and use of rice male sterility line

Info

Publication number: CN1042088C
Application number: CN92106933A
Authority: CN
Inventors: 陈绍光
Original assignee: Individual
Current assignee: Individual
Priority date: 1992-05-21
Filing date: 1992-05-21
Publication date: 1999-02-17
Anticipated expiration: 2007-05-21
Also published as: CN1066558A

Abstract

The present invention relates to a method for sieving and using a male sterility line of rice. With a method for sowing late rice (or semilate rice) in advance in different stages and batches, a proper sterile line and a sowing stage thereof can be sieved, and the late rice (or semilate rice) is outcrossed with early rice (or semilate rice) of a male parent, which blooms in the same stage, to obtain first generation hybrid seeds; when sowing is carried out according to the sowing season of normal late rice (or semilate rice), self cross fructification seed saving can be carried out. The present invention easily breeds hybridization seeds of a two-line method of the rice and uses the hybrid advantages of the hybridization seeds. Compared with the traditional three-line method, the two-line method has obvious economic benefits.

Description

Screen and utilize the method for male sterible series of rice

The present invention relates to a kind of method that obtains and utilize male sterible series of rice.

Hybrid rice three line method seeding technique and application thereof have been spread wide and have been obtained huge economic benefit and social benefit in China and each rice producing region, the world.Chinese Yuan Longping had early found the strain of abortion type male sterile in the Xian kind in the Dongting Lake in 1964, had opened the prelude of hybrid rice research.Japanese neofield length in 1986 has by Xian, round-grained rice hybridization have been bred BT type male sterile line and has realized three series mating in nineteen sixty-eight, but failed to drop into practical application.In the wild rice in Hainan Island, found the wild sterile strain of nature, and breed famous wild abortion type sterile series with the rice in China breeding men headed by the Yuan Longping in 1970, and then the three series mating of realizing long-grained nonglutinous rice in 1973, then japonica rice has also been realized three series mating.In 1973 later 10 years, China has successively bred numerous male sterile lines again, and has all realized three series mating.The hybrid rice three line method has been obtained remarkable achievement in the popularization and the application of China.Here said " three are " is meant male sterile line, male sterility maintainer line and male sterile restoring line, abbreviates male sterile line, maintenance line and recovery system respectively as.China is for finding male sterile line, screening maintenance line and recovering system, the strong excellent hybrid combination of seed selection, mobilized thousands of scientific worker and spent the time in 10 years, the program of the three line method production of hybrid seeds simultaneously complexity, first generation of hybrid seed production is low, seed costs is high, cost an arm and a leg.Thereby changing three line method is two line method, becomes the important subject of rice breeding men over nearly 10 years.

Photosensitive sterile material has been found in, the bright pine of Chinese Hubei stone in 1972 from land-reclaimable 58 kinds, from then on begun the very long exploration of two line method.Utilize photosensitive sterile line to realize that two is supporting in China at present, and small-scale test, exemplary plantation have been carried out in the field, still the practical application of large-scale production can not have high input, this is that its sterility is limited by temperature and can't stablizes because the discovery of the quick male sterile line of this light (temperature) depends on accidental opportunity.Recently, the Yuan Longping (sees " hybrid rice " 1992 (1): mention 1-4) that " in the research in previous stage; owing to be subjected to the limitation of material and condition; the degree of depth and the range of research are not enough; particularly cause the knowledge of regularity of fertility conversion not comprehensive to light, warm condition; thereby do not have correct guidance thought and technology path in the process of actual seed selection male sterile line, so that existing light, the temp-sensing sterile line overwhelming majority who breeds there is no practical value at " technical tactic of breeding rice photosensitive and temperature-sensitive sterile line " literary composition." therefore, screen fast and expeditiously and utilize stable Photosensitive male sterile line to become the focus of two line method tackling key problem.

Purpose of the present invention is exactly for a kind of method of the male sterile line of Screening of Rice fast and effectively is provided, such sterile lineage Photosensitive male sterile line, and fertility is easy to stablize; Another object of the present invention is to make the male sterile line that filters out be used for paddy rice two line method hybrid seeding, makes the two line method crossbreed can be applied to as early as possible produce.

This invention is based on following theory (can be referred to as male sterile ecological theory):

(1) stage of having grown of crop, logical party can be divided into vegetative growth phase and generative growth phase, desired light of each stage, warm primary condition difference.

(2) male sterile of crop maybe can be educated, both be limited by crop itself intrinsic hereditary capacity, be limited by again by the necessary light of generative growth phase, warm condition.

(3) male sterile of crop maybe can be educated, and is not absolute, but relative, can transform mutually under certain conditions.When crop can be met by the necessary light and temperature condition of generative growth phase, just can educate; Otherwise, will occur growing and stagnate or male sterile phenomenon.

(4) crop spermary official's growth, particularly pollen are easier to be subjected to the influence that ambient light, warm condition change and change and educate than the female organ of crop.

(5) the Photosensitive male sterile line is than the temp.-sensitive type male sterile line, and its sterile performance is more stable, thereby the value of utilization is more arranged.This is because day long variation is more stable than variation of temperature, thereby, strong from photosensitivity, to the responsive crop (or kind) of long reaction of day, more easily find the Photosensitive sterile material.

Based on above theory, we think that more easily finding can be for the photosensitive sterile section bar material that utilizes from late rice.Rice-cultivating is evolved from common wild-rice.The division of early rice, semilate rice, late rice is not the main morning and evening according to sowing season, but mainly according to its light and temperature characteristic.Light and temperature characteristic and the wild rice of late rice are more approaching, thereby late rice is basic model, and with regard to long-grained nonglutinous rice, japonica rice, the light and temperature characteristic of long-grained nonglutinous rice is more near wild rice, thereby long-grained nonglutinous rice is a basic model.The photosensitivity of late rice is strong, and is responsive to long reaction of day, is typical short day crop, thereby the easiest sterile section bar material of long day that finds, and early rice is to long reaction insensitiveness of day, and its male sterile is limited by temperature more, thereby value is low.Semilate rice is between late rice and early rice, and with regard to its light and temperature characteristic, slow ripe semilate rice levels off to the precocious semilate rice of late rice and then levels off to early rice.

The essence of this invention is exactly to utilize late rice or slow ripe semilate rice to the susceptibility of illumination, by doing sth. in advance the method for sowing, causes its male sterile.From local early rice sowing season, the left and right sides was a collection of in per 10 days, or broadcast in every month upper, middle and lower ten days a collection ofly, and sowing finds that for the late rice or the middle rice varieties of screening though the day in spring is long short, temperature is low excessively; Though and the temperature in summer is high, day is long, all can not satisfy its specific (special) requirements by the necessary short day high temperature of generative growth phase, weight person, the stagnation of growing, the maturation of can not in time earing; The lighter, the normally and only pollen abortion of growing, Here it is our needed male sterile line.After filtering out suitable late rice or middle rice varieties and suitable sowing time thereof, just can be by this sowing in sowing time, and can meet with the florescence, as the early rice or the relevant field planting of middle rice varieties of male parent, just can obtain first generation of hybrid seed by natural hybrization.Sow normal sowing season by local late rice or semilate rice as late rice or the semilate rice that will screen, but self-fertility and reserving seed for planting then, like this, same late rice or middle rice varieties, just can integrate male sterile line and maintenance line, change the purpose that three line method is a two line method thereby reached, make hybrid rice two line method seeding technique be implemented and bring benefit to the mankind.

The invention has the advantages that: (1) screening material wide material sources, the screening technique simple and feasible, thereby breakneck acceleration is fast; (2) can screen the Photosensitive male sterile line, be easy to stablize, thus effective; (3) make the two line method production of hybrid seeds be easier to realize.

Embodiment: in the area, Changsha, late March sowing late riceization is educated No. 4, and with No. 8 alternate field planting of early rice Hunan Xian morning of the sowing same period.To mid-June, change and to educate No. 4 and go out fringe and present male sterile, part fringe bagging, because of male sterile can not self-fertility: the fringe of bagging not because of accepting the Hunan pollen outcrossing of No. 8, Xian early, has obtained the first-filial generation seed.

Claims

1, the method for Screening of Rice male sterile line, it is characterized in that, to sow in advance as the rice varieties of late rice or middle rice culture in the locality, from local early rice sowing season, sowing by stages and in groups, screening male sterile and kind that female organ normally can be educated and cause this male sterile sowing time.

2, the method for Screening of Rice male sterile line according to claim 1 is characterized in that, sowing can be that sowing is a collection of every about 10 days by stages and in groups, and perhaps the upper, middle and lower ten days of every month is respectively broadcast a collection of.

3, the male sterible series of rice of said method screening utilize method, it is characterized in that it can be used for the production of hybrid seeds of paddy rice two line method, promptly by local late rice or the normal sowing in sowing time of semilate rice, but self-fertility reserve seed for planting, play the effect of maintenance line; By sowing in sowing time of screening, then be male sterile, can with obtain first-filial generation seed with the male parent early rice of planting or semilate rice cross-fertile therebetween.