CN1081887C - Selection of sterility lined with leaf marks for three way crossed rice - Google Patents
Selection of sterility lined with leaf marks for three way crossed rice Download PDFInfo
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- CN1081887C CN1081887C CN97109237A CN97109237A CN1081887C CN 1081887 C CN1081887 C CN 1081887C CN 97109237 A CN97109237 A CN 97109237A CN 97109237 A CN97109237 A CN 97109237A CN 1081887 C CN1081887 C CN 1081887C
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Abstract
The present invention discloses a selection method of sterility lined with leaf marks of three-line crossed paddy rice. In the method, the cross and the composite cross are carried out to the maintenance line of three-line cross paddy rice and marked rice, the test cross is respectively carried out to good single plants selected in the F2 and the sterility, the plants are continuously selected for the back cross until the circulation is carried out to the ninth generation, and then the sterility lined with leaf marks can be obtained; and the seeds have unique leaf marks, anti-counterfeiting functions and easy identification. The method can be used for greatly reducing the workloads for removing impurities and undesirables in fields in the process of seed production, and simultaneously can also obviously enhance the production level, the heterosis, the rice quality, the resistance, etc., and the method has great popularization value.
Description
Affiliated technical field
The present invention relates to a kind ofly utilize the rice leaf mark property to cultivate cytoplasmic male sterile rice and the new combination of hybrid rice makes its crossbreed have the new method of anti-counterfeit recognition function, belong to the Plant genetics technical field.
Background technology
At the beginning of 1970's, China successfully cultivates cytoplasmic male sterile rice on short bar breeding basis, and three series mating is lost in the very fast open country of having realized, indica hybrid rice combines plant forms improvement and the vigorous physiological function of the first generation of hybrid together, produce powerful hybrid vigour, the rapid popularization of hybrid rice makes the average per unit area yield of paddy rice improve 20%, for important contribution has been made in the grain-production of China.At present, China's hybrid rice sowing area accounts for paddy rice can plough the over half of area, and output accounts for 2/3 of paddy rice gross output.The success of indica hybrid rice is bred, and is described as the green revolution second time on the rice breeding history.
At the beginning of 1980's, the discovery of paddy rice light sensitive nuclear sterility and paddy rice wide compatibility gene is carried out inter-subspecific heterosis utilization research for China hybrid rice and is warded off new way in addition.Study in year surplus ten, inter-subspecific heterosis utilization between paddy rice photosensitive-sensitive sterile line and two line method kind has obtained important breakthrough, and two-line hybrid rice newly makes up the large-scale demonstration success, and the subspecies combination all shows stronger hybrid vigour between kind.
As everyone knows, at present, China produces the combination overwhelming majority who upward uses and makes up as wild abortion type, and cytoplasm is single, and composite type is few, and quality is bad partially, and resistance goes down, and advantage dies down, and light sensitive nuclear sterility line fertility shakiness is the continuation development of perplexing hybrid rice all the time.Because the purification and the discriminating of male-sterile seed have certain degree of difficulty, part seed operator and some individual illegal merchants are had an opportunity to take advantage of, mix the spurious with the genuine, with bad fill excellent, allow defective seed come into the market, cause enormous economic loss for grain farmer and country, also caused bad social influence.
The innovation and creation content
The object of the present invention is to provide a kind of selection with series of three-series hybrid rice male sterile line of leaf marking trait, adopt this method gained seed should have the anti-counterfeit recognition function, be easy to identification, identify and protect pure, simultaneously can also improve its output, resistance and Mi Zhi etc.
Be the technical measures that realize that above-mentioned purpose of the present invention is taked:
A kind of selection with series of three-series hybrid rice male sterile line of leaf marking trait, it comprises the steps:
1. utilize manual detasseling method, will have the good maintenance line hybridization combo of the mark seed rice and the ternary hybrid rice of leaf marking trait, obtain F
1In generation, show as neat and consistent, but leaf marking trait is not expressed plantation F
1Seed obtain F
2In generation, show as agronomy, leaf marking trait and be 3: 1 and separate.
2. from F
2Selecting to have the plant forms of leaf marking trait and the individual plant of comprehensive proterties deflection maintenance line in generation, and three is the transformation of male sterile line test cross, obtains the F behind the test cross
1In generation, show as neatly substantially, and leaf marking trait is not expressed.
3. observe to survey combo and close, eliminate not have and keep the survey combo of sterile ability to close, screening keep 100% allusion quotation lose sterile combination backcross B
1F
1, show as index blade and separate at 1: 1 with normal leaf; Simultaneously to possessing the hybridization three generations (F of hold facility
3) keep strain, reestablish diplomatic relations with former maintenance line, obtain the F that reestablishes diplomatic relations
4Generation.
4. continue to select strain pairing backcrossing male sterile line, select good maintenance line individual plant simultaneously, so be recycled to for the 9th generation (to obtain B respectively
6F
1And F
9), stable typing this moment, to the 9th generation gained seed be series of three-series hybrid rice seed with leaf marking trait condition.Its The Characters is for except that having new leaf marking trait, and other proterties and former maintenance material and sterile material are in full accord.
According to technical scheme of the present invention, described good maintenance is the good maintenance line of any ternary hybrid rice; Described mark seed rice is purple leaf rice, or is pale green leaf rice, or is the Ban Maye rice, and wherein preceding two kinds is natural kind, and the Ban Maye rice then is by the artificially breeding gained.
The present invention has proposed seed selection first and has had the method for the series of three-series hybrid rice male sterile line of leaf marking trait, the remarkable result with the following aspects:
1. because the gained seed has unique leaf marking, make seed have antiforge function, identification easily, can identify the true and false and the purity of seed at short notice by hot-house culture, save the evaluation fund, and the accuracy height of identifying, can avoid seed of poor quality to come into the market and the loss of causing for country and grain farmer.
2. utilize leaf marking trait, can alleviate workload and labour intensity that the field removal of impurities in the seed production process is rogued greatly, guarantee the purity of seed.
3. utilize the inventive method can improve the level of production, heterosis, hybrid vigor, adaptability and the rice matter and the resistance etc. of seed significantly, following table has been listed Zhenshan 97a, Shanyou 63 and by the average single rate (Kg/ mu) of the inventive method gained seed in different phase, as can be known from the table data, obviously be better than other two kinds by the inventive method gained seed, therefore have and apply potentiality greatly.
4. method maturation of the present invention, reliable and stable is easy to promote, and promotes the use of this method widely and will improve the Rice Production level, further promotes China's hybrid rice production health to develop steadily.
Description of drawings
Fig. 1 is seed selection flow process of the present invention and trait expression situation, and Fig. 2 is a Ban Maye rice artificially breeding flow process; Wherein/, X represent hybridization, F
1, F
2, F
3... F
9, F
10, F
11Be respectively two generations of a crossbreed generation, crossbreed, crossbreed three generations ... ten generations of nine generations of crossbreed, crossbreed, crossbreed ten generation, B
1F
1, B
2F
1... B
6F
1Be respectively first backcross generation, second backcross generation ... backcrossed for six generations.
Embodiment
Below in conjunction with specific embodiment technical scheme of the present invention is further described:
Embodiment:
Select No. 3, zebra leaf seed rice and Wu Jin, utilize manual detasseling method to hybridize combo, obtain F
1Generation, plantation F
1The seed in generation obtains F
2Generation.From F
2Select to have the fine individual plant of the plant forms and No. 3 maintenance lines of the military gold of comprehensive proterties deflection of zebra blade in generation, reestablish diplomatic relations with former maintenance line on the one hand,, obtain the F behind F3 and the test cross respectively on the other hand with the male sterile line test cross of No. 3, military gold
1At F
3In eliminate not have keep the survey combo of sterile ability to close, screening keeps 100% allusion quotation to lose sterile combination backcrossing and obtain B
1F
1, simultaneously to possessing the F of hold facility
3Keep plant to reestablish diplomatic relations and obtain the F that reestablishes diplomatic relations with No. 3 maintenance lines of former military gold
4Generation.Continue to select strain pairing backcrossing and Jia Dai and select maintenance line, be recycled to for the 9th generation continuously to obtain B respectively
6F
1Male sterile line and F
9Maintenance line, gained seed are No. 3 hybrid rice seeds of military gold with zebra leaf marking trait.
Result of the test shows: this material is discerned easily, is identified, stable fertility, and economical character is good, and the column cap prosperity is bloomed concentrated, and stronger heterosis, hybrid vigor and resistance are arranged, and rice is of fine quality, can be used for large tracts of land production.
Claims (2)
1. the selection with male sterile line in ternary hybrid rice of leaf marking trait is characterized in that this method comprises the steps:
A. utilize manual detasseling method, the good maintenance line hybridization with mark seed rice and ternary hybrid rice obtains first-filial generation F
1, plant F
1Seed obtain second filial generation F
2
B. from second filial generation F
2In select to have the plant forms of leaf marking trait and the individual plant of comprehensive proterties deflection maintenance line, and three be the first generation after sterile test cross transformation obtains test cross, reestablish diplomatic relations with former maintenance line simultaneously and obtain hybridizing three generations F
3, continue the improvement maintenance line;
C. from F
3Middle selection possesses the maintenance plant of hold facility, and reestablishing diplomatic relations with former maintenance line obtains hybridizing four generation F
4, simultaneously screening keeps 100% allusion quotation to lose sterile combination backcrossing and obtain first backcross generation B in the first generation behind test cross
1F
1
D. continue to select strain pairing backcrossing male sterile line, select fine individual plant simultaneously, be recycled to the male sterile line in ternary hybrid rice that is that the 9th generation obtained with leaf marking trait.
2. according to the described selection of claim 1, it is characterized in that described mark seed rice is purple leaf rice, or pale green leaf rice, or zebra leaf rice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97109237A CN1081887C (en) | 1997-09-10 | 1997-09-10 | Selection of sterility lined with leaf marks for three way crossed rice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97109237A CN1081887C (en) | 1997-09-10 | 1997-09-10 | Selection of sterility lined with leaf marks for three way crossed rice |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1185898A CN1185898A (en) | 1998-07-01 |
| CN1081887C true CN1081887C (en) | 2002-04-03 |
Family
ID=5171047
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN97109237A Expired - Fee Related CN1081887C (en) | 1997-09-10 | 1997-09-10 | Selection of sterility lined with leaf marks for three way crossed rice |
Country Status (1)
| Country | Link |
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| CN (1) | CN1081887C (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1085038C (en) * | 1998-02-18 | 2002-05-22 | 四川省农业科学院作物研究所 | Propagation method of rice leaf marker sterile line |
| CN1110248C (en) * | 1999-04-10 | 2003-06-04 | 安徽省农业科学院 | Application of molecular marking technique in quick determination of true or false and purity of hybrid rice seed |
| CN102405828A (en) * | 2011-08-19 | 2012-04-11 | 连云港市农业科学院 | Rapid breeding method of glutinous-rice sterile line of three lines |
| CN102668975A (en) * | 2012-06-11 | 2012-09-19 | 广西壮族自治区农业科学院水稻研究所 | Method for producing seed by using three-line hybrid rice sterile line with purple leaf marker |
| CN102668976B (en) * | 2012-06-11 | 2016-11-16 | 广西壮族自治区农业科学院水稻研究所 | A kind of high-yield breeding method of purple leaf hybrid rice CMS line |
| CN105706901B (en) * | 2016-01-29 | 2017-12-19 | 江西省农业科学院作物研究所 | With leaf margin totally cleave marking character rape sterile line and selection and application |
| CN110447530B (en) * | 2019-09-05 | 2021-01-08 | 中国水稻研究所 | Breeding method of light green leaf three-line sterile line, indica-japonica intermediate restorer line and indica-japonica subspecies hybrid rice |
| CN113475385A (en) * | 2021-07-20 | 2021-10-08 | 贺州市农业科学院 | Breeding method of sterile line of three-line hybrid rice with leaf marking property |
| CN113951131B (en) * | 2021-11-05 | 2022-08-09 | 广东省农业科学院水稻研究所 | Method for rapidly breeding three-line rice maintainer line and sterile line by using rice genome analysis technology |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1066558A (en) * | 1992-05-21 | 1992-12-02 | 陈绍光 | Screen and utilize the method for male sterible series of rice |
-
1997
- 1997-09-10 CN CN97109237A patent/CN1081887C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1066558A (en) * | 1992-05-21 | 1992-12-02 | 陈绍光 | Screen and utilize the method for male sterible series of rice |
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| Publication number | Publication date |
|---|---|
| CN1185898A (en) | 1998-07-01 |
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